CmoCh13G002110 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G002110
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMajor facilitator superfamily protein
LocationCmo_Chr13: 1631725 .. 1634272 (-)
RNA-Seq ExpressionCmoCh13G002110
SyntenyCmoCh13G002110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAGCGCCGGTGCGTTTTATTTGTATTCTCATTTCTCCAAAGACATTAAGGAAACGCTCCAATGTGACCAAACAACACTCAACAAGATTGGGTTTTACAAAGATATTGGATCAAACATCGACATTTTCTCTGTTCTACTTGTTGAAATTGCGCCATCTTGGGTCCTTCTCGTCATTTGCTCCGCGGTTAACTTCATTGGCTACTTCAAGATATGGCAAGGCGTCGTTGGAAAAATTGTGCAACCATCTGTTGAGTACTTTTGCTTTTACATAGCCATGGGGGGAAACTCTCAAATTTTAGCCAATTCAGGTAATGCAGGATGGTTGGGAGGGAGTCTCACTTTCGCTAATTTAGGAAATGATCATGAGTTTATGACGGTAGGAGACTTTTTGGGGAAGCCCAAAACAAGGCCATGAGAGGTTACGCTCAAAGTGGACAATATCATACCAGAAAGTCATGGTTCCTAACATGGTATTAGAGCCAGACATGGAGCGGTGTGCCAACGAGTAAGCTGGGCCCCTACAGCGAGTAAGCTGGGCCCCTAAAGGTGGTGGATTGTGAGATCTGACATTGATTGGAGAGAGGAACAAAACATTCTTAATGATGTGGAAACCTCTCCCTAGTGGACGCATTTTGGAAGCTCGGAAGGGAAAGCCCAAAGACCAAAGAGAACAATATTTGTTACTGGTTGGTTTGGGCGTCCTACATTGGCTAATTTTTTTAACGCCTTTTCGGGAAGCCCAAAACAAAACCAAACCCATGAGAGCTCAGGCTAAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTTCTAACATGGTATCAAAGCTAGACACTGAGCGTTGTGCCAGCGAGGACATTGGCCCCTAAAGGTGGTGGATTGTGAGATCCCACATTTGTTGGAGAGGGGAACAAAACATTCTTAAGGATGTGGAAACCTCTCCCTAGTAGACACGTTTTAAAACCTTAAGAGGAAGCCTGGAAGAGAAAGCCCAAAGACCAAAGAGAACAATATTTGTTAGTTGGTTTGGGCATCCCACATGGGCTAATTTTTCTTGAAGCCTTTTAGGAAAGTCCAAAAAAAACCAACTCATGAGAGCTTATGCTCAAAGTGGAGAATACCATATCATATGGATATATCATACCATTCCGAGTCGTACGATTAAAATAATGTCTCCTAATTAGCTTATAATTCTTTTTTGCTTTCAACAGTGGTGCTGGTGACATGTGTGAGGAACTTCCCGGAAAGAAGAGGGGTGATATTGGGGCTTTTGAAGGGCTTCTTAGGCCTAGGAGCGGCTGTACTGTCCCAAATTTACCTAGCCATTTATGGTCATGATACCAAATCTCTGATCCTGTTCATTGCGTGGTTTCCATCCTTGATTACTCTGATTTTTGCCTTTTCAATCAAGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGTTTCTATGCATCACTCTCATTTTAGCTATGTTTATCATAGTGTTAACCATTATTCAAAGAATAGTTCAATTTGATCAATCAGCACACATTATAGTGATGATTGCAATCATGGGTTTGCTAATTCTTCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCGTTGGAATCTTAACAAAATGACCAAATTTGAAAAATGCCAAACATCAGCCTCTATAATTTCATATTCCTCTTCCACATTACAACATTCAAGAAATTCAGATCTTTCAAATATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGATTTTTCAGCATTGACATGCTCCTCATATGCCTTACAATGTCAATTGGTATCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCAGCTGAATCTATCAATGCAATAGTCACCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTTTCCGGGTTCGTTTCAGAAATCTTGCTCGAACAATACAAATTTCCCCGACCCTTGATGCTGACACTAACCCTTTTGGTGTCGTGCATTGGTTACTTATTGGTGGCCTTCCCCTTTAATAATTCACTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCGTAATTTCAGAGATTTTTGGTCTTAAACATTACGCTATATTTTATAACATTGCGCAACTCTCTTGCCCAATTGGGACTTATGTTCTAAACGTGTTGGTGGCTGGGAAACTCTACGACAAGGAAGTGAAGACATGGATTGATTCAAGTGAATATAAATGCCATGGGGAACAATGTTACAAAGATTCTTTCACGATTTTGACAGGGATGTCGTTGTTGGGAGCTGCAATATCGTTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGAGGGGATATATACAGGAAGTTTAGGGAAGATATGGATTCTTTGAGGGAGGAAGTGGAACTTTACTCTCTTGATGTGAGGCAGAATGAAGATGAAAGCGTACATGTCCATAAAGAAGTTACCATTTTCAAGAAATGA

mRNA sequence

ATGACAAGCGCCGGTGCGTTTTATTTGTATTCTCATTTCTCCAAAGACATTAAGGAAACGCTCCAATGTGACCAAACAACACTCAACAAGATTGGGTTTTACAAAGATATTGGATCAAACATCGACATTTTCTCTGTTCTACTTGTTGAAATTGCGCCATCTTGGGTCCTTCTCGTCATTTGCTCCGCGGTTAACTTCATTGGCTACTTCAAGATATGGCAAGGCGTCGTTGGAAAAATTGTGCAACCATCTGTTGAGTACTTTTGCTTTTACATAGCCATGGGGGGAAACTCTCAAATTTTAGCCAATTCAGTGGTGCTGGTGACATGTGTGAGGAACTTCCCGGAAAGAAGAGGGGTGATATTGGGGCTTTTGAAGGGCTTCTTAGGCCTAGGAGCGGCTGTACTGTCCCAAATTTACCTAGCCATTTATGGTCATGATACCAAATCTCTGATCCTGTTCATTGCGTGGTTTCCATCCTTGATTACTCTGATTTTTGCCTTTTCAATCAAGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGTTTCTATGCATCACTCTCATTTTAGCTATGTTTATCATAGTGTTAACCATTATTCAAAGAATAGTTCAATTTGATCAATCAGCACACATTATAGTGATGATTGCAATCATGGGTTTGCTAATTCTTCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCGTTGGAATCTTAACAAAATGACCAAATTTGAAAAATGCCAAACATCAGCCTCTATAATTTCATATTCCTCTTCCACATTACAACATTCAAGAAATTCAGATCTTTCAAATATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGATTTTTCAGCATTGACATGCTCCTCATATGCCTTACAATGTCAATTGGTATCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCAGCTGAATCTATCAATGCAATAGTCACCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTTTCCGGGTTCGTTTCAGAAATCTTGCTCGAACAATACAAATTTCCCCGACCCTTGATGCTGACACTAACCCTTTTGGTGTCGTGCATTGGTTACTTATTGGTGGCCTTCCCCTTTAATAATTCACTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCGTAATTTCAGAGATTTTTGGTCTTAAACATTACGCTATATTTTATAACATTGCGCAACTCTCTTGCCCAATTGGGACTTATGTTCTAAACGTGTTGGTGGCTGGGAAACTCTACGACAAGGAAGTGAAGACATGGATTGATTCAAGTGAATATAAATGCCATGGGGAACAATGTTACAAAGATTCTTTCACGATTTTGACAGGGATGTCGTTGTTGGGAGCTGCAATATCGTTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGAGGGGATATATACAGGAAGTTTAGGGAAGATATGGATTCTTTGAGGGAGGAAGTGGAACTTTACTCTCTTGATGTGAGGCAGAATGAAGATGAAAGCGTACATGTCCATAAAGAAGTTACCATTTTCAAGAAATGA

Coding sequence (CDS)

ATGACAAGCGCCGGTGCGTTTTATTTGTATTCTCATTTCTCCAAAGACATTAAGGAAACGCTCCAATGTGACCAAACAACACTCAACAAGATTGGGTTTTACAAAGATATTGGATCAAACATCGACATTTTCTCTGTTCTACTTGTTGAAATTGCGCCATCTTGGGTCCTTCTCGTCATTTGCTCCGCGGTTAACTTCATTGGCTACTTCAAGATATGGCAAGGCGTCGTTGGAAAAATTGTGCAACCATCTGTTGAGTACTTTTGCTTTTACATAGCCATGGGGGGAAACTCTCAAATTTTAGCCAATTCAGTGGTGCTGGTGACATGTGTGAGGAACTTCCCGGAAAGAAGAGGGGTGATATTGGGGCTTTTGAAGGGCTTCTTAGGCCTAGGAGCGGCTGTACTGTCCCAAATTTACCTAGCCATTTATGGTCATGATACCAAATCTCTGATCCTGTTCATTGCGTGGTTTCCATCCTTGATTACTCTGATTTTTGCCTTTTCAATCAAGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGTTTCTATGCATCACTCTCATTTTAGCTATGTTTATCATAGTGTTAACCATTATTCAAAGAATAGTTCAATTTGATCAATCAGCACACATTATAGTGATGATTGCAATCATGGGTTTGCTAATTCTTCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCGTTGGAATCTTAACAAAATGACCAAATTTGAAAAATGCCAAACATCAGCCTCTATAATTTCATATTCCTCTTCCACATTACAACATTCAAGAAATTCAGATCTTTCAAATATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGATTTTTCAGCATTGACATGCTCCTCATATGCCTTACAATGTCAATTGGTATCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCAGCTGAATCTATCAATGCAATAGTCACCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTTTCCGGGTTCGTTTCAGAAATCTTGCTCGAACAATACAAATTTCCCCGACCCTTGATGCTGACACTAACCCTTTTGGTGTCGTGCATTGGTTACTTATTGGTGGCCTTCCCCTTTAATAATTCACTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCGTAATTTCAGAGATTTTTGGTCTTAAACATTACGCTATATTTTATAACATTGCGCAACTCTCTTGCCCAATTGGGACTTATGTTCTAAACGTGTTGGTGGCTGGGAAACTCTACGACAAGGAAGTGAAGACATGGATTGATTCAAGTGAATATAAATGCCATGGGGAACAATGTTACAAAGATTCTTTCACGATTTTGACAGGGATGTCGTTGTTGGGAGCTGCAATATCGTTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGAGGGGATATATACAGGAAGTTTAGGGAAGATATGGATTCTTTGAGGGAGGAAGTGGAACTTTACTCTCTTGATGTGAGGCAGAATGAAGATGAAAGCGTACATGTCCATAAAGAAGTTACCATTTTCAAGAAATGA

Protein sequence

MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVICSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKEVTIFKK
Homology
BLAST of CmoCh13G002110 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.7e-20
Identity = 127/540 (23.52%), Postives = 232/540 (42.96%), Query Frame = 0

Query: 3   SAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVICS 62
           S G  + +S +S  +K  L   Q  LN +    D+G      S + +   P  V+L   +
Sbjct: 56  STGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAA 115

Query: 63  AVNFIGYFKIWQGVVGKIVQP-SVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGVI 122
           A+ F+GY   W  +   I  P S+ + C  +A  G S    N+   + C+R+FP  R + 
Sbjct: 116 AMGFVGYGVQWLVITNIITLPYSLVFLCCLLA--GLSICWFNTACFILCIRHFPNNRALA 175

Query: 123 LGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVK---- 182
           L L   F G+ AA+ S  + AI    +   +L      SL+ L+ +F+     + K    
Sbjct: 176 LSLTVSFNGISAALYSLAFNAINPSSSNLYLL----LNSLVPLVVSFAALYPVLTKPSLD 235

Query: 183 -------HPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILP 242
                    H+  VF     + +I +  +++ +      + +    +++++     L  P
Sbjct: 236 TTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLVF---PLCAP 295

Query: 243 LFIAIRE---EMVRWNLNK------MTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNK 302
           L +  R+    ++   LN       M   ++ +   + +S S +  +H   +   N    
Sbjct: 296 LLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVS-SKTGYEHMGTAKEGNTV-- 355

Query: 303 PERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTII 362
              GD+++       ++  L  +    G    L   +NLGQI +S G   ++   +VTI 
Sbjct: 356 -RLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVTIY 415

Query: 363 SIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFN--NSLYIASIL 422
           S F+F GR+ S    + + ++++  R     + LL + I + L+A   +   +L  A+ L
Sbjct: 416 SSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATAL 475

Query: 423 IGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVK--- 482
           IG + G      +++ S++FG     + +NI   + PIG+ +L   +A  +Y+       
Sbjct: 476 IGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIYEANASPDI 535

Query: 483 TWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSL 517
           T I S    C G  CY  +F     +S+LG   SL L  RT+      +Y +  +D  SL
Sbjct: 536 TPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK-----PVYHRLEQDKVSL 572

BLAST of CmoCh13G002110 vs. ExPASy TrEMBL
Match: A0A6J1HLV5 (uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC111464764 PE=4 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.2e-300
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI
Sbjct: 40  MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 99

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV
Sbjct: 100 CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 159

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH
Sbjct: 160 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 219

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV
Sbjct: 220 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 279

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML
Sbjct: 280 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 339

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL
Sbjct: 340 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 399

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG
Sbjct: 400 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 459

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT
Sbjct: 460 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 519

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE
Sbjct: 520 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 579

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 580 VTIFKK 585

BLAST of CmoCh13G002110 vs. ExPASy TrEMBL
Match: A0A6J1I3Q6 (uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 7.5e-247
Identity = 451/546 (82.60%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFY YS+FS+ IKETLQCDQTTLNKI FYKDIGSNIDI S LLV IAPSWVLL+I
Sbjct: 28  MTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALLVCIAPSWVLLLI 87

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSA+NF+GYFKIWQ VVG++V P+VEYF  YIAM GNSQILAN+VVLV CVRNFPERRGV
Sbjct: 88  CSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQILANTVVLVICVRNFPERRGV 147

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVL+Q+YLAIYGHDTKSLILFIAW PSLITL+FAFSI+EVKIVKHPH
Sbjct: 148 ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLLFAFSIREVKIVKHPH 207

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQ LCITL+LA+F+ VLTI +++VQFDQ A+  V++AI+GLLILPLFIAIREEMV
Sbjct: 208 EFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMV 267

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           + NLN MTKFEK ++   I  ++ S  Q+  NS  SNIFNKPERGDDYTILQG FSIDML
Sbjct: 268 QLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNSYYSNIFNKPERGDDYTILQGIFSIDML 327

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICL MSIG GSSLTAMDNLGQIGESQGYS ES+NA+V+IISIFNFLGRIFSGFVSEILL
Sbjct: 328 LICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILL 387

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           E+YKFPRPLMLTLTLLVSCIG+LLVAFPF+NSLYIASILIGFAFGSQVLLDIT+ISE FG
Sbjct: 388 EKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFG 447

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           L+HYA+FYN+AQL+CPIGTYVL VLVAGKLYD+EVKTW DS EY CHGEQCY++SFT LT
Sbjct: 448 LEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLT 507

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
            MSLLGAAISLILVKRTREFYR DIY+KFREDMDSL+EEVELY  DVRQ E ESVHVHKE
Sbjct: 508 EMSLLGAAISLILVKRTREFYRQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKE 567

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 568 VTIFKK 573

BLAST of CmoCh13G002110 vs. ExPASy TrEMBL
Match: A0A6J1EYR7 (uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC111440576 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.1e-242
Identity = 442/546 (80.95%), Postives = 492/546 (90.11%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLY++F + IKETLQCDQTTLNKI FYKDIGSNID+ S LLV IAPSW+LL+I
Sbjct: 28  MTSAGAFYLYAYFPEHIKETLQCDQTTLNKISFYKDIGSNIDVVSALLVYIAPSWILLLI 87

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSA+NFIGYFKIWQ VVG+I  P+VEYF  YIA+ GNSQILAN+VVLV CVRNFPERRG+
Sbjct: 88  CSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIAIAGNSQILANTVVLVICVRNFPERRGM 147

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVL+Q+YLAIYGHDTKSLILFIAW PSLITL FAFSI+EVKIVKHPH
Sbjct: 148 ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLFFAFSIREVKIVKHPH 207

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQ LCITL+LA+F+ VLTI +++VQFDQ A+  V++AI+GLLILPLFIAIREEMV
Sbjct: 208 EFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMV 267

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           + NLN M KFEK Q+      ++ S  Q+  NS  SNIFNKPERGDDYTILQGFFSIDML
Sbjct: 268 QLNLNNMIKFEKSQSKPQRPIHAISHNQNHGNSHYSNIFNKPERGDDYTILQGFFSIDML 327

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLT+SIG GSSLTAMDNLGQIGESQGYS ES+NA+V+IISIFNF+GRIFSGFVSEILL
Sbjct: 328 LICLTISIGFGSSLTAMDNLGQIGESQGYSTESVNAMVSIISIFNFVGRIFSGFVSEILL 387

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           E+YKFPRPLMLTLTLLVSCIG+LLVAFPF+NSLYIASILIGF+FGSQVLLDIT+ISEIFG
Sbjct: 388 EKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASILIGFSFGSQVLLDITMISEIFG 447

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           L+HYA+FYNIAQLSCPIGTYVL VLVAGKLYD+ VKTW D +EY CHGEQCY+ SFT LT
Sbjct: 448 LEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYDEAVKTWSDPTEYICHGEQCYRSSFTFLT 507

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           G+SLLGAAISLILVKRTREFYR DIY+KFREDMDSL+EEVELY  DVRQ E ES+HVH+E
Sbjct: 508 GISLLGAAISLILVKRTREFYREDIYKKFREDMDSLKEEVELYPHDVRQPEVESIHVHQE 567

Query: 541 VTIFKK 547
            TI KK
Sbjct: 568 GTIIKK 573

BLAST of CmoCh13G002110 vs. ExPASy TrEMBL
Match: A0A6J1I0L4 (uncharacterized protein LOC111469419 OS=Cucurbita maxima OX=3661 GN=LOC111469419 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 2.1e-201
Identity = 376/453 (83.00%), Postives = 413/453 (91.17%), Query Frame = 0

Query: 94  MGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLIL 153
           M GNSQILAN+VVLV CVRNFPERRGVILGLLKGFLGLGAAVL+Q+YLAIYGHDTKSLIL
Sbjct: 1   MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 60

Query: 154 FIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQ 213
           FIAW PSLITL+FAFSI+EVKIVKHPHEFRVFIQ LCITL+LA+F+ VLTI +++VQFDQ
Sbjct: 61  FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 120

Query: 214 SAHIIVMIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSASIISYSSSTLQHSRNS 273
            A+  V++AI+GLLILPLFIAIREEMV+ NLN MTKFEK ++   I  ++ S  Q+  NS
Sbjct: 121 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 180

Query: 274 DLSNIFNKPERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAES 333
             SNIFNKPERGDDYTILQG FSIDMLLICL MSIG GSSLTAMDNLGQIGESQGYS ES
Sbjct: 181 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 240

Query: 334 INAIVTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSL 393
           +NA+V+IISIFNFLGRIFSGFVSEILLE+YKFPRPLMLTLTLLVSCIG+LLVAFPF+NSL
Sbjct: 241 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 300

Query: 394 YIASILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDK 453
           YIASILIGFAFGSQVLLDIT+ISE FGL+HYA+FYN+AQL+CPIGTYVL VLVAGKLYD+
Sbjct: 301 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 360

Query: 454 EVKTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDM 513
           EVKTW DS EY CHGEQCY++SFT LT MSLLGAAISLILVKRTREFYR DIY+KFREDM
Sbjct: 361 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 420

Query: 514 DSLREEVELYSLDVRQNEDESVHVHKEVTIFKK 547
           DSL+EEVELY  DVRQ E ESVHVHKEVTIFKK
Sbjct: 421 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 453

BLAST of CmoCh13G002110 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.7e-188
Identity = 340/557 (61.04%), Postives = 439/557 (78.82%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MT AG  YL++++S+DIK TLQCDQTTLN+IGFYKD+GSN+ + + LL E+AP+W++L+I
Sbjct: 65  MTGAGGVYLFAYYSRDIKTTLQCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLI 124

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
            +A NFIGYFKIWQ V GKIV+P+V +FCFYI +G NSQ  AN+ VLVTCV+NFPERRGV
Sbjct: 125 GAAFNFIGYFKIWQAVTGKIVRPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGV 184

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           +LGLLKGF+GL  A+++Q+Y+AIYGHDTKSLIL IAWFPS+++LIF ++I+E+K VKHP+
Sbjct: 185 MLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPN 244

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVF+QFLC+T++LA+ +  L  +Q+ V FDQSAHI ++ AI+ LL +PL IAIREE+V
Sbjct: 245 EFRVFVQFLCVTVLLALLLTALIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVV 304

Query: 241 RWNLNKMTKFEKCQTSASIIS-------YSSSTLQH----SRNSDLSNIFNKPERGDDYT 300
            WNLNK T          I S       +S ST QH       S  SNIFNKPERG+DYT
Sbjct: 305 IWNLNKRTPISNPFIRIGIESSQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYT 364

Query: 301 ILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGR 360
           +LQ  FSIDML+IC TM IG+G+SLTA+DNLGQIGE+QGY +E+IN  V++ISI NF GR
Sbjct: 365 VLQAIFSIDMLIICSTMLIGVGASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGR 424

Query: 361 IFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVL 420
           IFSGFVSEILLE+++FPRPLMLTL LL+S +G+LLVAFPF++SLY+ASI+IGF+ GSQV 
Sbjct: 425 IFSGFVSEILLEKFQFPRPLMLTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVP 484

Query: 421 LDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGE 480
           L   +ISEIFGLKHY+  +N  QLSCPIG+Y+LNV+V GKLYD+  +   + ++  C G 
Sbjct: 485 LHFAMISEIFGLKHYSTLFNFGQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGT 544

Query: 481 QCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQ 540
            CY+ SF IL G++ + A ISLILV+RTREFYRGDIY+KF+EDM++L+ EVE Y +D ++
Sbjct: 545 HCYEQSFLILAGLTFIVAMISLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKR 604

Query: 541 NEDESVHVHKEVTIFKK 547
               ++ V K    FKK
Sbjct: 605 TRIGNLLVDKHSINFKK 621

BLAST of CmoCh13G002110 vs. NCBI nr
Match: XP_022964763.1 (uncharacterized protein LOC111464764 [Cucurbita moschata])

HSP 1 Score: 1041.6 bits (2692), Expect = 2.4e-300
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI
Sbjct: 40  MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 99

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV
Sbjct: 100 CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 159

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH
Sbjct: 160 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 219

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV
Sbjct: 220 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 279

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML
Sbjct: 280 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 339

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL
Sbjct: 340 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 399

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG
Sbjct: 400 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 459

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT
Sbjct: 460 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 519

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE
Sbjct: 520 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 579

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 580 VTIFKK 585

BLAST of CmoCh13G002110 vs. NCBI nr
Match: KAG6583520.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034.6 bits (2674), Expect = 3.0e-298
Identity = 542/546 (99.27%), Postives = 544/546 (99.63%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEI+PSWVLLVI
Sbjct: 40  MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEISPSWVLLVI 99

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSAVNFIGYFKIWQGVVG+IVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV
Sbjct: 100 CSAVNFIGYFKIWQGVVGQIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 159

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH
Sbjct: 160 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 219

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV
Sbjct: 220 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 279

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
            WNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML
Sbjct: 280 HWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 339

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL
Sbjct: 340 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 399

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG
Sbjct: 400 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 459

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT
Sbjct: 460 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 519

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQ EDESVHVHKE
Sbjct: 520 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQKEDESVHVHKE 579

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 580 VTIFKK 585

BLAST of CmoCh13G002110 vs. NCBI nr
Match: XP_023521066.1 (uncharacterized protein LOC111784665 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007.7 bits (2604), Expect = 3.9e-290
Identity = 524/546 (95.97%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLYSHFSKDIKETL+CDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSW+LLVI
Sbjct: 40  MTSAGAFYLYSHFSKDIKETLKCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWILLVI 99

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFY+AMGGNSQILANSVVLVTCVRNFPERRGV
Sbjct: 100 CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYMAMGGNSQILANSVVLVTCVRNFPERRGV 159

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH
Sbjct: 160 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 219

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVF+QFLCITLILA+F+IVLTIIQRIV+FDQSAHI+VMIAIMGLLILPLFIAIREEMV
Sbjct: 220 EFRVFVQFLCITLILAIFLIVLTIIQRIVEFDQSAHILVMIAIMGLLILPLFIAIREEMV 279

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
            WNLNKMTKFEKCQTSASIISYSSSTLQHSRNS LSNIFNKPERGDDYTILQG FSIDML
Sbjct: 280 HWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSYLSNIFNKPERGDDYTILQGIFSIDML 339

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLTMSIGIGSSLTAMDNLGQIGESQGYSA+SINAIV+IISIFNF+GRIFSGFVSEILL
Sbjct: 340 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAQSINAIVSIISIFNFIGRIFSGFVSEILL 399

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           EQYKFPRPLMLTLTLLVSCIGYLLVAFPF NSLYIASILIGFAFGSQVLLDIT+ISEIFG
Sbjct: 400 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFYNSLYIASILIGFAFGSQVLLDITIISEIFG 459

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWI SSEYKCHGEQCY+DSFTILT
Sbjct: 460 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIASSEYKCHGEQCYRDSFTILT 519

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           GMSLLGAAISLILVKRTREFYRGDIY+KFREDMDSLREEVELYSLD RQNE ES+HVHKE
Sbjct: 520 GMSLLGAAISLILVKRTREFYRGDIYQKFREDMDSLREEVELYSLDERQNEAESIHVHKE 579

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 580 VTIFKK 585

BLAST of CmoCh13G002110 vs. NCBI nr
Match: XP_022970393.1 (uncharacterized protein LOC111469393 [Cucurbita maxima])

HSP 1 Score: 862.8 bits (2228), Expect = 1.5e-246
Identity = 451/546 (82.60%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFY YS+FS+ IKETLQCDQTTLNKI FYKDIGSNIDI S LLV IAPSWVLL+I
Sbjct: 28  MTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALLVCIAPSWVLLLI 87

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSA+NF+GYFKIWQ VVG++V P+VEYF  YIAM GNSQILAN+VVLV CVRNFPERRGV
Sbjct: 88  CSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQILANTVVLVICVRNFPERRGV 147

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVL+Q+YLAIYGHDTKSLILFIAW PSLITL+FAFSI+EVKIVKHPH
Sbjct: 148 ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLLFAFSIREVKIVKHPH 207

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQ LCITL+LA+F+ VLTI +++VQFDQ A+  V++AI+GLLILPLFIAIREEMV
Sbjct: 208 EFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMV 267

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           + NLN MTKFEK ++   I  ++ S  Q+  NS  SNIFNKPERGDDYTILQG FSIDML
Sbjct: 268 QLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNSYYSNIFNKPERGDDYTILQGIFSIDML 327

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICL MSIG GSSLTAMDNLGQIGESQGYS ES+NA+V+IISIFNFLGRIFSGFVSEILL
Sbjct: 328 LICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILL 387

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           E+YKFPRPLMLTLTLLVSCIG+LLVAFPF+NSLYIASILIGFAFGSQVLLDIT+ISE FG
Sbjct: 388 EKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFG 447

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           L+HYA+FYN+AQL+CPIGTYVL VLVAGKLYD+EVKTW DS EY CHGEQCY++SFT LT
Sbjct: 448 LEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLT 507

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
            MSLLGAAISLILVKRTREFYR DIY+KFREDMDSL+EEVELY  DVRQ E ESVHVHKE
Sbjct: 508 EMSLLGAAISLILVKRTREFYRQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKE 567

Query: 541 VTIFKK 547
           VTIFKK
Sbjct: 568 VTIFKK 573

BLAST of CmoCh13G002110 vs. NCBI nr
Match: KAG6583517.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850.9 bits (2197), Expect = 6.1e-243
Identity = 443/546 (81.14%), Postives = 492/546 (90.11%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           MTSAGAFYLYS+F + IKETLQCDQTTLNKI FYKDIGSNID+ S LLV IAPSW+LL+I
Sbjct: 41  MTSAGAFYLYSYFPEHIKETLQCDQTTLNKISFYKDIGSNIDVVSALLVYIAPSWILLLI 100

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
           CSA+NFIGYFKIWQ VVG+I  P+VEYF  YIA+ GNSQILAN+VVLV CVRNFPERRGV
Sbjct: 101 CSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIAIAGNSQILANTVVLVICVRNFPERRGV 160

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           ILGLLKGFLGLGAAVL+Q+YLAIYGHDTKSLILFIAW PSLITL FAFSI+EVKIVKHPH
Sbjct: 161 ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLFFAFSIREVKIVKHPH 220

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           EFRVFIQ LCITL+LA+F+ VLTI +++VQFDQ A+  V++AI+GLLILPLFIAIREEMV
Sbjct: 221 EFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMV 280

Query: 241 RWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDML 300
           + NLN M KFEK Q+      ++ S  Q+  NS  SNIFNKPERGDDYTILQGFFSIDML
Sbjct: 281 QLNLNNMIKFEKSQSKPQRPIHAISHNQNHGNSHYSNIFNKPERGDDYTILQGFFSIDML 340

Query: 301 LICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILL 360
           LICLT+SIG GSSLTAMDNLGQIGESQGYS ES+NA+V++ISIFNF+GRIFSGFVSEILL
Sbjct: 341 LICLTISIGFGSSLTAMDNLGQIGESQGYSTESVNAMVSVISIFNFVGRIFSGFVSEILL 400

Query: 361 EQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFG 420
           E+YKFPRPLMLTLTLLVSCIG+LLVAFPF+NSLYIASILIGF+FGSQVLLDIT+ISEIFG
Sbjct: 401 EKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASILIGFSFGSQVLLDITMISEIFG 460

Query: 421 LKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILT 480
           L+HYA+FYNIAQLSCPIGTYVL VLVAGKLYD+ VKTW D +EY CHGEQCY+ SFT LT
Sbjct: 461 LEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYDEAVKTWSDPTEYICHGEQCYRSSFTFLT 520

Query: 481 GMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKE 540
           G+SLLGAAISLILVKRTREFYR DIY+KFREDMDSL+EEVELY  DVRQ E ES+HVH+E
Sbjct: 521 GISLLGAAISLILVKRTREFYREDIYKKFREDMDSLKEEVELYPHDVRQPEVESIHVHQE 580

Query: 541 VTIFKK 547
            TI KK
Sbjct: 581 GTIIKK 586

BLAST of CmoCh13G002110 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 498.4 bits (1282), Expect = 7.2e-141
Identity = 261/538 (48.51%), Postives = 367/538 (68.22%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           M  AGA YL+  +SKDIK TL  DQTTLN +GF+KD+G+N+ + S L+ E+ P+W +L I
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
            SA+NF+GYF IW  V GK+ +P V   C YI +G NSQ  AN+  LVTCV+NFPE RGV
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           +LGLLKG++GL  A+ +Q+Y AIYGHD+KSLIL IAW P+ ++L+F + I+E K+V+  +
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVYLIREKKVVRQRN 207

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           E  VF QFL I++ LA+F++ + I ++ V F ++A+         LL +PL +++++E+ 
Sbjct: 208 ELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQELE 267

Query: 241 RWNLNKM-------TKFEKCQTSASIISYSSSTL---QHSRNSDLSNIFNKPERGDDYTI 300
            WN+ K+        K EK +    +    ++ +   +    S  S +F+ P RG+DYTI
Sbjct: 268 VWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPPPRGEDYTI 327

Query: 301 LQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNFLGRI 360
           LQ   S DM+++ +    G+GSSLTA+DNLGQIGES GY   ++++ V+++SI+N+ GR+
Sbjct: 328 LQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFGRV 387

Query: 361 FSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLL 420
           FSGFVSE LL +YK PRPLM+TL LL+SC G+LL+AFP   S+YIASIL+GF+FG+Q+ L
Sbjct: 388 FSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLPL 447

Query: 421 DITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEV--------KTWIDSS 480
              +ISE+FGLK+Y+  +N  QL+ P+G+Y+LNV V G LYDKE          T  D  
Sbjct: 448 LFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTARGLTRKDVK 507

Query: 481 EYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDMDSLREEV 521
           +  C G QCYK  F IL  ++  GA +SL L  RTREFY+GDIY+KFRE  +S  E V
Sbjct: 508 DLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRESPESESELV 565

BLAST of CmoCh13G002110 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 5.3e-128
Identity = 248/549 (45.17%), Postives = 357/549 (65.03%), Query Frame = 0

Query: 1   MTSAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVI 60
           M++AGA Y++  +S DIKETL  DQTTLN + F+KD+G+N+ + + LL E+ P W +L+I
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  CSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGV 120
            + +NF GYF IW  V  +I +P V + C YI +G NSQ  AN+  LVTCV+NFPE RGV
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 ILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPH 180
           +LG+LKG++GL  A+++Q+Y A YG DTK LIL I W P++++  F  +I+ +K+ +  +
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTN 211

Query: 181 EFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMV 240
           E +VF  FL I+L LA F++V+ II ++  F QS        ++ LL+LP+ + I EE  
Sbjct: 212 ELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEKK 271

Query: 241 RWN-----------LNKMTKFEKCQTSASIISYSSSTLQHSRN--------SDLSNIFNK 300
            W            +N +T+  K  +S     +     + S+         S  + +FN 
Sbjct: 272 LWKEKQVALNDPAPINVVTEKPKLDSS----EFKDDDGEESKEVVEKVKTPSCWTTVFNP 331

Query: 301 PERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTII 360
           PERGDDYTILQ  FS+DML++ L    G+G +LTA+DNLGQIG S GY   S++  V+++
Sbjct: 332 PERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLV 391

Query: 361 SIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIG 420
           SI+N+ GR+ SG VSEI L +YKFPRPLMLT+ LL+SC G+LL+AF     LY+AS++IG
Sbjct: 392 SIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIG 451

Query: 421 FAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEV------ 480
           F FG+Q  L   +ISEIFGLK+Y+  YN   ++ PIG+Y+LNV VAG LYD E       
Sbjct: 452 FCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKA 511

Query: 481 --KTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRKFREDM 523
             KT ++  +  C G  C+K SF I+  ++L G  +S++LV RT++FY+ DIY+KFRE  
Sbjct: 512 LGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFRE-- 571

BLAST of CmoCh13G002110 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 4.3e-69
Identity = 167/525 (31.81%), Postives = 287/525 (54.67%), Query Frame = 0

Query: 3   SAGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPS-------- 62
           ++GA Y +  +S  +K T   DQ+TL+ +  +KDIG+N  +FS LL   A S        
Sbjct: 20  ASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLYTYATSNRLRGRGG 79

Query: 63  --------WVLLVICSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVV 122
                   WV+L + +   F GYF IW  V G I +P V   C ++ +   SQ   N+  
Sbjct: 80  GIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTAN 139

Query: 123 LVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITL-- 182
           +V+ V NF +  G  +G++KGFLGL  A+L Q+Y  +   D  S IL +A  P++++L  
Sbjct: 140 VVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFILLLAVTPTVLSLLV 199

Query: 183 -----IFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIV 242
                I+  S+ + K  KH +          ++LI+A +++++ I++        A+I+ 
Sbjct: 200 MPLVRIYETSVADDK--KHLNGLS------AVSLIIAAYLMIIIILKNTFGLSSWANIVT 259

Query: 243 MIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIF 302
           ++ ++ +L LPL IA R +  R  + K    +     + +IS   +T   +++S+     
Sbjct: 260 LVCLLVMLALPLLIARRAQ--RDGMEKTVPHD----YSPLISSPKATTSGNQSSEGD--- 319

Query: 303 NKPERG--DDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAI 362
           +K E G  ++  +LQ    +   L+ L M  G+GS L+ ++N+ QIGES  YS+  IN++
Sbjct: 320 SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSL 379

Query: 363 VTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIAS 422
           V++ SI+NFLGR  +G+ S+ LL +  +PRPL++  TL    IG+L++A  F  +LY+ S
Sbjct: 380 VSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGS 439

Query: 423 ILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKT 482
           +++G  +GSQ  L  T+ SE+FG++H    +N   ++ PIG+Y+ +V + G +YDK    
Sbjct: 440 VIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTA-- 499

Query: 483 WIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYR 503
                   C+G  C++ SF I+  ++  G  ++++L  RT+  YR
Sbjct: 500 --SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYR 523

BLAST of CmoCh13G002110 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 3.1e-67
Identity = 171/522 (32.76%), Postives = 280/522 (53.64%), Query Frame = 0

Query: 4   AGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPS--------W 63
           +GA Y ++ +S  +K +   DQ+TL+ +  +KDIG    I S  L     S        W
Sbjct: 21  SGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLYTAMTSKSRGFGGPW 80

Query: 64  VLLVICSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFP 123
           V++ +     F+G+F IW  VVG I  P V   C ++ + G+S    N+  +VT  RNF 
Sbjct: 81  VVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPFFNTANVVTAARNFS 140

Query: 124 ERRGVILGLLKGFLGLGAAVLSQIYLAIYGHD--TKSLILFIAWFPSLITLI---FAFSI 183
           +  G  +G+++GFLGL  A+L Q+Y A+ G +    + IL +A  P+L+  +   F    
Sbjct: 141 QYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVPTLVMFLAMPFVRVY 200

Query: 184 KEVKIVKHPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGLLILP 243
           + V I    H          I++I+A +++V+  ++ ++   +S  I   I ++ LL  P
Sbjct: 201 ETVTISDKKH----LDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFSFILVLLLLASP 260

Query: 244 LFIAIREEMVRWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSD--LSNIFNKPER--GD 303
           L +A+R              EK QT +S+       L  S   D   SNIF   +    +
Sbjct: 261 LLVAVR-----------ALREKRQTLSSL---DGPVLDTSALLDPPSSNIFPDGDHLVAE 320

Query: 304 DYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNF 363
           D  IL+   +++  L+ L M  G+GS    ++N+ QIGES  YS+  +N++V++ SI+NF
Sbjct: 321 DSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNF 380

Query: 364 LGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGS 423
           LGR  +G+VS+  L ++ +PRP+ + +TL V  IG+++VA     SLY  S+LIG A+GS
Sbjct: 381 LGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGS 440

Query: 424 QVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKC 483
           Q  L  T+ SEIFG++H    Y    ++ PIG+Y+L+V V G  YDK         +  C
Sbjct: 441 QWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVA----SEDDNSC 500

Query: 484 HGEQCYKDSFTILTGMSLLGAAISLILVKRTREFYRGDIYRK 509
            G QC++ SF I+  ++L G+ ++ +L  RT +FY+  + ++
Sbjct: 501 FGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of CmoCh13G002110 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 8.9e-67
Identity = 160/515 (31.07%), Postives = 277/515 (53.79%), Query Frame = 0

Query: 4   AGAFYLYSHFSKDIKETLQCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPS--------- 63
           +GA Y +  +S  +K +   DQ+TL+ +  YKDIG+N+ I S L      S         
Sbjct: 21  SGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGLFYTAVASRKSGNGGFF 80

Query: 64  ---WVLLVICSAVNFIGYFKIWQGVVGKIVQPSVEYFCFYIAMGGNSQILANSVVLVTCV 123
              W+++ +     F+GY  IW    G I +P V   C ++   G+ Q   N+ ++VT V
Sbjct: 81  SGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFAGHCQPFFNTAIVVTAV 140

Query: 124 RNFPERRGVILGLLKGFLGLGAAVLSQIYLAIYGHDTKSLILFIAWFPSLITLIFAFSIK 183
           RNF +  G  +G++KG+LGL  A+L Q+Y    G D ++ IL +A  PSL+ L     ++
Sbjct: 141 RNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLLAVVPSLLILTLMPFVR 200

Query: 184 EVKIV-----KHPHEFRVFIQFLCITLILAMFIIVLTIIQRIVQFDQSAHIIVMIAIMGL 243
               V     KH +          I+LI+  +++V+ +++ I+       I     ++ L
Sbjct: 201 TYDTVIAGDKKHLNGLS------AISLIIVTYLMVVILVENIIGMSMPMKICSFTFLLLL 260

Query: 244 LILPLFIAIREEMVRWNLNKMTKFEKCQTSASIISYSSSTLQHSRNSDLSNIFNKPERGD 303
           L  PL +A+R +  R   ++    +   T  + +  S      + +SD+ ++       +
Sbjct: 261 LASPLLVAVRAQ--REEEHRFLSLDFPVTERTTLLDSPKL---NSSSDVKDVMT-----N 320

Query: 304 DYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLGQIGESQGYSAESINAIVTIISIFNF 363
           D  +L+   + +  L+ + M  G+GS L  ++N+ Q+GES  YS   +N++V++ SI+NF
Sbjct: 321 DMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSIWNF 380

Query: 364 LGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIGYLLVAFPFNNSLYIASILIGFAFGS 423
           LGR  SG++S+  L  + +PRP+ + +TL +  IG++++A     SLYI S+L+G A+GS
Sbjct: 381 LGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLAYGS 440

Query: 424 QVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYVLNVLVAGKLYDKEVKTWIDSSEYKC 483
           Q  L  T+ SEIFG+ H    +    ++ P+G+Y  +V V G LYDK         ++ C
Sbjct: 441 QWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVA----SEDDHSC 500

Query: 484 HGEQCYKDSFTILTGMSLLGAAISLILVKRTREFY 502
           +G  C++ SF I+  M+LLG+ ++L+L+ RT++FY
Sbjct: 501 YGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFY 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E12.7e-2023.52Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HLV51.2e-300100.00uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I3Q67.5e-24782.60uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393... [more]
A0A6J1EYR71.1e-24280.95uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC1114405... [more]
A0A6J1I0L42.1e-20183.00uncharacterized protein LOC111469419 OS=Cucurbita maxima OX=3661 GN=LOC111469419... [more]
A0A5D3BP482.7e-18861.04Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022964763.12.4e-300100.00uncharacterized protein LOC111464764 [Cucurbita moschata][more]
KAG6583520.13.0e-29899.27Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023521066.13.9e-29095.97uncharacterized protein LOC111784665 [Cucurbita pepo subsp. pepo][more]
XP_022970393.11.5e-24682.60uncharacterized protein LOC111469393 [Cucurbita maxima][more]
KAG6583517.16.1e-24381.14Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT2G28120.17.2e-14148.51Major facilitator superfamily protein [more]
AT2G39210.15.3e-12845.17Major facilitator superfamily protein [more]
AT1G74780.14.3e-6931.81Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.13.1e-6732.76Major facilitator superfamily protein [more]
AT2G34350.18.9e-6731.07Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 506..533
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 1..516
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 1..516
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 295..502
e-value: 9.6E-14
score: 53.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 55..231
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 201..499
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 1..235
e-value: 6.0E-62
score: 209.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G002110.1CmoCh13G002110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity