CmoCh13G001240 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G001240
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description(1->3)-beta-glucan endohydrolase
LocationCmo_Chr13: 719477 .. 721600 (+)
RNA-Seq ExpressionCmoCh13G001240
SyntenyCmoCh13G001240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCTCCATTTTTCCCTCTCCTCCTCAGCCTCTTCTCTCTCTTCTTCCGACTTACCTCCGCCGCCTACTCCATCGGCGTCAACTATGGCACGGTAGCCGACAACCTCCCTCCGCCGTTCCAGGTCGCTGCCTTCCTCAAATCCCAAACCTCCATCGATCGCGTCAAGATCTTTGACGCCAACCCCGATATCCTTCGCGCCTTCGCCGGCACCGGCATCGCCCTCACTATTTCCGTCGTTAACGGTGACATTCCAACCCTCGCCAAACTCCCCGCCGCTCAGTCTTGGGTCGCCATTAACGTTCTTCCGTTTTACCCCAACACTCTGATCAACTACATCGCCGTCGGGAATGAGATTCTCGCCACTTCCGATAAGAATCTCATAGCGCATATGCTCCCGGCGATGAAGGCTTTACATTCCGCTCTGAGGCTCGCGAATATCTCGGACATCAAGGTTTCAACCCCTCATTCGTTGGGCATTTTGTCGGCGTCGGAGCCGCCGAGTAACGGGCGGTTCCGGCGGGGGTACGACCGTGTCATATTTGCTCCGATCCTCGAGTTTCACCGTCGGACCAAGACGCCGTTCATGGTGAATCCGTACCCCTTTTTTAGTTTCAGACCGGCGACGCTGGACTTCGCGCTGTTTAAGCCCAACGCCGGGGTTTTCGACAACGTCACTGGAATGCACTACACGAACATGTTCGACGCGCAGCTGGATGCGGTTTACTCGGCGATGAAGAAGCTCGGTTACGGCGACGTGGACATCGTGGTGGCGGAGACTGGTTGGCCCTCCGCCGGCGACCCAAATCAACCCGGCGTGAGCATGGAGAACGCCATTTCGTACAACCGGAATCTCGTTAAGCTTGTCAACTCCGGTATCGGAACGCCATTGATGCCGAACAGAACCTTCGAAACTTACGTCTTCGCTCTTTTCAACGAGAATCTCAAACCGTCGGCTTCTGAGAGGAACTACGGCCTTTTCAAACCCGATCTAACTCCGGTCTACGACGTCGGCATCATCCTGAACCAACCGGTAAACCGCTTCTCCTTGTAGAAAAAACCCATACCGCCTTAGCCGGACTGAGGTCCGAATACGGCGGGGGCATTCTAGTAAATGTACATCAAGCACACGCGTAGTAAACAGAACGTCATTGGTCATGTACACGTGTTGATTCCTAATTGATTTATGGCTCTACCTGTAAGCTTGTTGTTTTGCAACCAATAAAAAATCGACGCGTAGGAGTCGCACCAAGCCAGCTGTCCAAGAAAGCCAAAGCCACGTGTTCTTTTTCTATTTGGACGGAATTACCCTACATTTTATTCTTTTTATCATGGCAATATAGTCAATTTGTTTTTCTTTTCTATTTTTGTTTATTTTTTAATGAATTGCAGACGAACTTAACCGTTGATTTTAATGTAACAGTCAATGGGGCCCACGTCTCCAAACGCACACGCACCAGAACCGTTATATGTGGGGAGGAAGTGGTGCGTGCCGAAAACGGACGTGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGCGGAGTGGAGTGTGGGCCCATAGAAGCCGGCGGGGCCTGCTACAATCCCAATACAGTCCGGTCACATGCTTCGTACGCCATGAACGCTTACTTTCAGTCCGCCGGCCGCCATGATTTCAACTGCGACTTTAACAACACCGCCATCCTCACCGCCGCCGACCCCAGTAAGATTTTCTTCTTCTTCTTCTCCCTTTAAATTCCGGTTAAAATTTCAATGGAATTTACAGGTTATGAGACCTGCAGCTACCCGTTCGATGAAGAAAAGTTAACCGAAAAATCGGCGGGGATGACCAGTAGGATGATCGGGGCGGTGGCGCTCGCATGTATTGCGGTGGTTGGTGTGGCGGTGTTTTACTAATTTGAAACAAAAGTGAGATTTGGTGTTTTTTTTTTTATATAATTTACATACAAAACTCAATTAATGTAAAGCTTGAAGGAGTTAAATAACAATGAAAAATATTCCCTCTACGCTTAATAATCACTCCATGCATATCGCGCAGCTCCGATCGTCACTGCGGCGGCCGGGGAATCTTCTTCTGTCTGCCGCCGTGCTCCTCGCGAGTTATAA

mRNA sequence

ATGGCTTCCTCTCCATTTTTCCCTCTCCTCCTCAGCCTCTTCTCTCTCTTCTTCCGACTTACCTCCGCCGCCTACTCCATCGGCGTCAACTATGGCACGGTAGCCGACAACCTCCCTCCGCCGTTCCAGGTCGCTGCCTTCCTCAAATCCCAAACCTCCATCGATCGCGTCAAGATCTTTGACGCCAACCCCGATATCCTTCGCGCCTTCGCCGGCACCGGCATCGCCCTCACTATTTCCGTCGTTAACGGTGACATTCCAACCCTCGCCAAACTCCCCGCCGCTCAGTCTTGGGTCGCCATTAACGTTCTTCCGTTTTACCCCAACACTCTGATCAACTACATCGCCGTCGGGAATGAGATTCTCGCCACTTCCGATAAGAATCTCATAGCGCATATGCTCCCGGCGATGAAGGCTTTACATTCCGCTCTGAGGCTCGCGAATATCTCGGACATCAAGGTTTCAACCCCTCATTCGTTGGGCATTTTGTCGGCGTCGGAGCCGCCGAGTAACGGGCGGTTCCGGCGGGGGTACGACCGTGTCATATTTGCTCCGATCCTCGAGTTTCACCGTCGGACCAAGACGCCGTTCATGGTGAATCCGTACCCCTTTTTTAGTTTCAGACCGGCGACGCTGGACTTCGCGCTGTTTAAGCCCAACGCCGGGGTTTTCGACAACGTCACTGGAATGCACTACACGAACATGTTCGACGCGCAGCTGGATGCGGTTTACTCGGCGATGAAGAAGCTCGGTTACGGCGACGTGGACATCGTGGTGGCGGAGACTGGTTGGCCCTCCGCCGGCGACCCAAATCAACCCGGCGTGAGCATGGAGAACGCCATTTCGTACAACCGGAATCTCGTTAAGCTTGTCAACTCCGGTATCGGAACGCCATTGATGCCGAACAGAACCTTCGAAACTTACGTCTTCGCTCTTTTCAACGAGAATCTCAAACCGTCGGCTTCTGAGAGGAACTACGGCCTTTTCAAACCCGATCTAACTCCGGTCTACGACGTCGGCATCATCCTGAACCAACCGTCAATGGGGCCCACGTCTCCAAACGCACACGCACCAGAACCGTTATATGTGGGGAGGAAGTGGTGCGTGCCGAAAACGGACGTGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGCGGAGTGGAGTGTGGGCCCATAGAAGCCGGCGGGGCCTGCTACAATCCCAATACAGTCCGGTCACATGCTTCGTACGCCATGAACGCTTACTTTCAGTCCGCCGGCCGCCATGATTTCAACTGCGACTTTAACAACACCGCCATCCTCACCGCCGCCGACCCCAGTTATGAGACCTGCAGCTACCCGTTCGATGAAGAAAAGTTAACCGAAAAATCGGCGGGGATGACCAGTAGGATGATCGGGGCGGTGGCGCTCGCATCTCCGATCGTCACTGCGGCGGCCGGGGAATCTTCTTCTGTCTGCCGCCGTGCTCCTCGCGAGTTATAA

Coding sequence (CDS)

ATGGCTTCCTCTCCATTTTTCCCTCTCCTCCTCAGCCTCTTCTCTCTCTTCTTCCGACTTACCTCCGCCGCCTACTCCATCGGCGTCAACTATGGCACGGTAGCCGACAACCTCCCTCCGCCGTTCCAGGTCGCTGCCTTCCTCAAATCCCAAACCTCCATCGATCGCGTCAAGATCTTTGACGCCAACCCCGATATCCTTCGCGCCTTCGCCGGCACCGGCATCGCCCTCACTATTTCCGTCGTTAACGGTGACATTCCAACCCTCGCCAAACTCCCCGCCGCTCAGTCTTGGGTCGCCATTAACGTTCTTCCGTTTTACCCCAACACTCTGATCAACTACATCGCCGTCGGGAATGAGATTCTCGCCACTTCCGATAAGAATCTCATAGCGCATATGCTCCCGGCGATGAAGGCTTTACATTCCGCTCTGAGGCTCGCGAATATCTCGGACATCAAGGTTTCAACCCCTCATTCGTTGGGCATTTTGTCGGCGTCGGAGCCGCCGAGTAACGGGCGGTTCCGGCGGGGGTACGACCGTGTCATATTTGCTCCGATCCTCGAGTTTCACCGTCGGACCAAGACGCCGTTCATGGTGAATCCGTACCCCTTTTTTAGTTTCAGACCGGCGACGCTGGACTTCGCGCTGTTTAAGCCCAACGCCGGGGTTTTCGACAACGTCACTGGAATGCACTACACGAACATGTTCGACGCGCAGCTGGATGCGGTTTACTCGGCGATGAAGAAGCTCGGTTACGGCGACGTGGACATCGTGGTGGCGGAGACTGGTTGGCCCTCCGCCGGCGACCCAAATCAACCCGGCGTGAGCATGGAGAACGCCATTTCGTACAACCGGAATCTCGTTAAGCTTGTCAACTCCGGTATCGGAACGCCATTGATGCCGAACAGAACCTTCGAAACTTACGTCTTCGCTCTTTTCAACGAGAATCTCAAACCGTCGGCTTCTGAGAGGAACTACGGCCTTTTCAAACCCGATCTAACTCCGGTCTACGACGTCGGCATCATCCTGAACCAACCGTCAATGGGGCCCACGTCTCCAAACGCACACGCACCAGAACCGTTATATGTGGGGAGGAAGTGGTGCGTGCCGAAAACGGACGTGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGCGGAGTGGAGTGTGGGCCCATAGAAGCCGGCGGGGCCTGCTACAATCCCAATACAGTCCGGTCACATGCTTCGTACGCCATGAACGCTTACTTTCAGTCCGCCGGCCGCCATGATTTCAACTGCGACTTTAACAACACCGCCATCCTCACCGCCGCCGACCCCAGTTATGAGACCTGCAGCTACCCGTTCGATGAAGAAAAGTTAACCGAAAAATCGGCGGGGATGACCAGTAGGATGATCGGGGCGGTGGCGCTCGCATCTCCGATCGTCACTGCGGCGGCCGGGGAATCTTCTTCTGTCTGCCGCCGTGCTCCTCGCGAGTTATAA

Protein sequence

MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNEILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQLDAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEPLYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYFQSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVTAAAGESSSVCRRAPREL
Homology
BLAST of CmoCh13G001240 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.0e-131
Identity = 232/459 (50.54%), Postives = 317/459 (69.06%), Query Frame = 0

Query: 8   PLLLSLFSLFFRLTSA-----AYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDA 67
           PLL+ L  L      A     +  IGVNYG  ADNLP P  VA FL ++T+IDRVK+FDA
Sbjct: 2   PLLILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDA 61

Query: 68  NPDILRAFAGTGIALTISVVNGDIPTLAK----LPAAQSWVAINVLPFYPNTLINYIAVG 127
           NP  + AFAGT I+L +S+ N  +P LA     L AA+SW+  N+ P+ P T +  +  G
Sbjct: 62  NPTFISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAG 121

Query: 128 NEILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEP-PSNGRFRRG 187
           NEIL ++D NLI  +LPAM+ L  AL+   ++ ++V+TPH LGIL+ S+  PSN  FR G
Sbjct: 122 NEILLSTDTNLILSLLPAMRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAG 181

Query: 188 YDRVIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFD 247
           Y+  +F  +L+FHR T +PFMVNPYP+FS+RP TL++ALF+PN+G++D  T ++YT+M D
Sbjct: 182 YNTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLD 241

Query: 248 AQLDAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGT 307
           AQ+DA+Y+AMKKLGYGDVDI V E GWP+  +P Q GV ++ A  +N  ++++ +SG GT
Sbjct: 242 AQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGT 301

Query: 308 PLMPNRTFETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPT----- 367
           PLMPNRTFETY+F+LF+EN KP   +ER++GLF PD TPVYD+G++ +  S+ PT     
Sbjct: 302 PLMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNP 361

Query: 368 SPNAHAPEPLYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSH 427
           SPN         G KWCV K   +   LQ NI+Y C   V+C PI++GGAC++PN++++H
Sbjct: 362 SPNPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAH 421

Query: 428 ASYAMNAYFQSAGRHDFNCDFNNTAILTAADPSYETCSY 451
           ASY MNAY+Q+ G  D  CDF  T I+T++DPSY  C Y
Sbjct: 422 ASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of CmoCh13G001240 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 8.1e-120
Identity = 220/448 (49.11%), Postives = 302/448 (67.41%), Query Frame = 0

Query: 11  LSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILRAF 70
           L +F   F  + A   IGVNYG VADNLPPP +    L+S TSI +V+++ A+P I++A 
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKAL 69

Query: 71  AGTGIALTISVVNGDIPTLAKLP-AAQSWVAINVLPFYPNTLINYIAVGNEILATSDKNL 130
           AGTG+ + I   NGD+P+LA  P AA  W+  NVLPFYP + I  I VGNEIL ++D NL
Sbjct: 70  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNL 129

Query: 131 IAHMLPAMKALHSALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPILE 190
           +  +LPAM+ +  AL   ++   IKVST +S+ +L +S+PPS+G F  GY   +   IL+
Sbjct: 130 VNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQ 189

Query: 191 FHRRTKTPFMVNPYPFFSF----RPATLDFALFKPNAGVFDNVTGMHYTNMFDAQLDAVY 250
           F   T +PF +NPYPFF++    RP TL F LF+PNAG  D+ TG+ YTNMFDAQ+DAV+
Sbjct: 190 FLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVH 249

Query: 251 SAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRT 310
           SA+K +G+  V+IVVAETGW S GD N+ G S++NA +YN NL+  + S +GTPLMP + 
Sbjct: 250 SALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKP 309

Query: 311 FETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEPLYV 370
            +TY+FAL++ENLKP  +SER +GLFK DL+ VYDVG       +  +S ++  P     
Sbjct: 310 VDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVG-------LAKSSSSSQTPSGKVT 369

Query: 371 GRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYFQSA 430
              WCVPK   ++E LQ ++D+ C  G++CG I+ GGAC+ PN V SHA+YAMN YFQ +
Sbjct: 370 SSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKS 429

Query: 431 GRHDFNCDFNNTAILTAADPSYETCSYP 452
            +   +CDF+ TA +T+ +PSY  C YP
Sbjct: 430 PKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of CmoCh13G001240 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.9e-117
Identity = 213/450 (47.33%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 9   LLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILR 68
           L+  L   F+   S ++ +GVNYG ++DNL P  Q    L   T+I +V++F A P +++
Sbjct: 13  LVFLLLQNFYSANSQSF-LGVNYGQLSDNL-PSLQATVNLLKSTTIQKVRLFGAEPAVIK 72

Query: 69  AFAGTGIALTISVVNGDIPTLAKLP-AAQSWVAINVLPFYPNTLINYIAVGNEILATSDK 128
           AFA TG+ + I   NGDIPTLA  P  A  +V  NV+ FYP + I  I VGNE+L + D+
Sbjct: 73  AFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQ 132

Query: 129 NLIAHMLPAMKALHSALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPI 188
            LI+ +LPAM+ + +AL  A++   +KVST H++ +LS S PPS+G F  G    + A +
Sbjct: 133 KLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKA-L 192

Query: 189 LEFHRRTKTPFMVNPYPFFSFR----PATLDFALFKPNAGVFDNVTGMHYTNMFDAQLDA 248
           L+F      PFM++PYP+F+++    P TL F LF+PNAG  D+  G  YTNMFDAQ+DA
Sbjct: 193 LQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDA 252

Query: 249 VYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPN 308
           V+SA+  +G+ D++IVVAETGWP  GD N+ G S++NA +Y  NL+  + S +GTPLMP 
Sbjct: 253 VHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPG 312

Query: 309 RTFETYVFALFNENLKPSA-SERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEPL 368
           ++ +TY+F+L++E+ K  A SE+ +GLFKPD +  YDVG++ N  +  PT+P    P P 
Sbjct: 313 KSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKN--TQNPTTPATPTPTPK 372

Query: 369 YVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYFQ 428
             G  WCVPK  VSD+ L  NI+Y C  G++CGPI+ GGAC+ PNTV++HA+Y MN Y+Q
Sbjct: 373 AAG-SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQ 432

Query: 429 SAGRHDFNCDFNNTAILTAADPSYETCSYP 452
           SAGR+ +NCDF+ TA LT  +PSY  C++P
Sbjct: 433 SAGRNSWNCDFSQTATLTNTNPSYGACNFP 456

BLAST of CmoCh13G001240 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 9.0e-87
Identity = 177/455 (38.90%), Postives = 266/455 (58.46%), Query Frame = 0

Query: 24  AYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILRAFAGTGIALTISVVN 83
           A  IG+ YG  ADNLP P +V+  ++   +I  V+I+DAN D+L+AFA TGI L I V N
Sbjct: 24  ASKIGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVLKAFANTGIELMIGVPN 83

Query: 84  GDIPTLAKLPA-AQSWVAINVLPFYPNTLINYIAVGNEILATSDKNLIAHMLPAMKALHS 143
            D+   A+  +   +W++ N+LP+YP+T I  I+VG E+    D N    +LPAM+ +H+
Sbjct: 84  ADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPD-NATGLVLPAMRNIHT 143

Query: 144 ALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPILEFHRRTKTPFMVNP 203
           AL+ + +   IK+S+ HSL ILS S PPS+  F + +      P+LEF    ++PFM++ 
Sbjct: 144 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENESPFMIDL 203

Query: 204 YPFFSFRPAT----LDFALFKPNAGVFDNVTGMHYTNMFDAQLDAVYSAMKKLGYGDVDI 263
           YP++++R +T    L++ALF+ ++ V D  TG+ Y+NMFDAQLDA+Y A+  + +  V +
Sbjct: 204 YPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKV 263

Query: 264 VVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRTFETYVFALFNENL 323
           +V E+GWPS G P +   + ENA++YN NL++ V    GTP  P    + Y+F+LFNEN 
Sbjct: 264 MVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENR 323

Query: 324 KPS-ASERNYGLFKPDLTPVYDVGII-LNQPSMGPT------------SPNAHAPEPLYV 383
           KP   SERN+G+F  + T VY +     N   + PT            SP  +    + +
Sbjct: 324 KPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTI 383

Query: 384 G-------RKWCVPKTDVSDEALQKNIDYVCSSG-VECGPIEAGGACYNPNTVRSHASYA 443
           G       +KWC+  +  S   LQ  +D+ C  G V+C  ++    C+ P+TV SHASYA
Sbjct: 384 GGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYA 443

Query: 444 MNAYFQSAGRHDFNCDFNNTAILTAADPSYETCSY 451
            N Y+Q +G    +C FN  ++    DPSY  C Y
Sbjct: 444 FNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of CmoCh13G001240 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.0e-82
Identity = 171/349 (49.00%), Postives = 219/349 (62.75%), Query Frame = 0

Query: 10  LLSLFSL-FFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILR 69
           LL L SL    L +   SIGVNYG + DNLP P  V   +KS     +VK++DANP IL+
Sbjct: 11  LLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKS-IGATKVKLYDANPQILK 70

Query: 70  AFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNEILATSDKN 129
           AF+ TGI   I + N  +  +     A +W+  NV PF P T I  I +GNEILA +D +
Sbjct: 71  AFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSS 130

Query: 130 LIAHMLPAMKALHSALRLANISD-IKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPIL 189
           L  ++LPAM+ +HSAL  A +SD I V+T HSL IL +S PPS G F+      +  PIL
Sbjct: 131 LTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSL-TPIL 190

Query: 190 EFHRRTKTPFMVNPYPFFSF----RPATLDFALFKPNAGVFDNVTGMHYTNMFDAQLDAV 249
           EFHR+T +PF++N YPFF++    +   LDF LF+PN G+ D  TG HY NM  AQ+DAV
Sbjct: 191 EFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAV 250

Query: 250 YSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSG--IGTPLMP 309
           YSA+   G+  + + ++ETGWPS GD ++ G + ENA  YN NL+K++ SG    TPL P
Sbjct: 251 YSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKP 310

Query: 310 NRTFETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMG 350
           N     YVFALFNENLKP   SERNYGLFKPD T  Y +G  LN    G
Sbjct: 311 NNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRG 357

BLAST of CmoCh13G001240 vs. ExPASy TrEMBL
Match: A0A6J1HLU3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111464794 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.4e-276
Identity = 477/483 (98.76%), Postives = 477/483 (98.76%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE
Sbjct: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
           LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF
Sbjct: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVT 480
           QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALA   V 
Sbjct: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALACIAVV 480

Query: 481 AAA 484
             A
Sbjct: 481 GVA 483

BLAST of CmoCh13G001240 vs. ExPASy TrEMBL
Match: A0A6J1HZC8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111469479 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.5e-262
Identity = 455/483 (94.20%), Postives = 462/483 (95.65%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLF RLTSAAYSIGVNYGTVADNLPPP QVAAFLKSQTSI+RVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFVRLTSAAYSIGVNYGTVADNLPPPSQVAAFLKSQTSINRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DAN DILRAFAGTGIALTISV NGDIPTL+KLPAAQSWVAIN+LPFYPNTLINYIAVGNE
Sbjct: 61  DANQDILRAFAGTGIALTISVANGDIPTLSKLPAAQSWVAINILPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDK LIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKILIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFF FRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFGFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGY DVDIVVAETGWPSAGDPNQPGVS+ENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYEDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGI+LNQPSMGPTSPNA A EP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGILLNQPSMGPTSPNAQASEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
             VGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIE+GGACYNPNTVRSHASYAMNAYF
Sbjct: 361 SDVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIESGGACYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVT 480
           QSAGRHDFNCDFNNTA+LTA DPSYETCSYPFDEEKLTEKSA MT RMIGAVAL    V 
Sbjct: 421 QSAGRHDFNCDFNNTALLTATDPSYETCSYPFDEEKLTEKSAAMTCRMIGAVALTCFAVV 480

Query: 481 AAA 484
             A
Sbjct: 481 GVA 483

BLAST of CmoCh13G001240 vs. ExPASy TrEMBL
Match: A0A5D3BDL6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002220 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.2e-230
Identity = 399/485 (82.27%), Postives = 434/485 (89.48%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MA S F  LLL LF  F  L+SAAYSIGVNYGTVA+NLPPP QVAAFLKS+TSIDRVKIF
Sbjct: 37  MAYSLFLSLLL-LFFFFIPLSSAAYSIGVNYGTVANNLPPPTQVAAFLKSRTSIDRVKIF 96

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALT+S+ NGDIP LAKLPAAQSWVA N+LPF+P TLIN+IAVGNE
Sbjct: 97  DANPDILRAFAGTGIALTVSIANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNE 156

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAH+LPAMKA+HSAL+LANISD+KVSTPHSLGILSASEPPS GRFRRGYDR
Sbjct: 157 ILATSDKNLIAHLLPAMKAIHSALKLANISDVKVSTPHSLGILSASEPPSTGRFRRGYDR 216

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
            IFAPIL+FH +TKTPFMVNPYPFF FRPATLD+ALFKPNAGVFDN TG HYTNMFDAQL
Sbjct: 217 AIFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYALFKPNAGVFDNATGKHYTNMFDAQL 276

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAV+SAMKK+G+GDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVK VNSG GTPLM
Sbjct: 277 DAVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKHVNSGKGTPLM 336

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETY+F+LFNENLKP+ SERNYGLF+PD TPVYDVGI+ N P+MGPTSP    P+P
Sbjct: 337 PNRTFETYIFSLFNENLKPTTSERNYGLFQPDFTPVYDVGILRNPPTMGPTSP-VGGPKP 396

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
              GR+WCVPKTD SDEALQKNIDYVCSSGV+CGPI+ GGACY+PNTVRSHASYAMNAYF
Sbjct: 397 SDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGACYDPNTVRSHASYAMNAYF 456

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKS----AGMTSRMIGAVALAS 480
           Q+AGRHDFNCDFN+TAILT  DPSYE CSYPFDEEKL EKS    A +   M+GAVA+A 
Sbjct: 457 QTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEEKLAEKSVASAATIRCSMVGAVAVAV 516

Query: 481 PIVTA 482
            +  A
Sbjct: 517 AVARA 519

BLAST of CmoCh13G001240 vs. ExPASy TrEMBL
Match: A0A1S3C6X2 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497159 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.2e-230
Identity = 399/485 (82.27%), Postives = 434/485 (89.48%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MA S F  LLL LF  F  L+SAAYSIGVNYGTVA+NLPPP QVAAFLKS+TSIDRVKIF
Sbjct: 1   MAYSLFLSLLL-LFFFFIPLSSAAYSIGVNYGTVANNLPPPTQVAAFLKSRTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALT+S+ NGDIP LAKLPAAQSWVA N+LPF+P TLIN+IAVGNE
Sbjct: 61  DANPDILRAFAGTGIALTVSIANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAH+LPAMKA+HSAL+LANISD+KVSTPHSLGILSASEPPS GRFRRGYDR
Sbjct: 121 ILATSDKNLIAHLLPAMKAIHSALKLANISDVKVSTPHSLGILSASEPPSTGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
            IFAPIL+FH +TKTPFMVNPYPFF FRPATLD+ALFKPNAGVFDN TG HYTNMFDAQL
Sbjct: 181 AIFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYALFKPNAGVFDNATGKHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAV+SAMKK+G+GDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVK VNSG GTPLM
Sbjct: 241 DAVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKHVNSGKGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETY+F+LFNENLKP+ SERNYGLF+PD TPVYDVGI+ N P+MGPTSP    P+P
Sbjct: 301 PNRTFETYIFSLFNENLKPTTSERNYGLFQPDFTPVYDVGILRNPPTMGPTSP-VGGPKP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
              GR+WCVPKTD SDEALQKNIDYVCSSGV+CGPI+ GGACY+PNTVRSHASYAMNAYF
Sbjct: 361 SDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGACYDPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKS----AGMTSRMIGAVALAS 480
           Q+AGRHDFNCDFN+TAILT  DPSYE CSYPFDEEKL EKS    A +   M+GAVA+A 
Sbjct: 421 QTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEEKLAEKSVASAATIRCSMVGAVAVAV 480

Query: 481 PIVTA 482
            +  A
Sbjct: 481 AVARA 483

BLAST of CmoCh13G001240 vs. ExPASy TrEMBL
Match: A0A0A0LVC1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.4e-230
Identity = 400/488 (81.97%), Postives = 433/488 (88.73%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MA S FF LLL     F  L+SAAYSIGVNYGTVA+NLPPP QVAAFLKS TSIDRVKIF
Sbjct: 1   MAYSLFFSLLL---FFFIPLSSAAYSIGVNYGTVANNLPPPSQVAAFLKSHTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALT+SV NGDIP LAKLPAAQSWVA N+LPF+P TLIN+IAVGNE
Sbjct: 61  DANPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAH+LPAMKA+HSAL+LA+ISD+KVSTPHSLGILSASEPPS GRFRRGYDR
Sbjct: 121 ILATSDKNLIAHLLPAMKAIHSALKLAHISDVKVSTPHSLGILSASEPPSTGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
            IFAPIL+FH +TKTPFMVNPYP+F FRPATLD+ALFKPN GVFDN TG HYTNMFDAQL
Sbjct: 181 AIFAPILDFHNQTKTPFMVNPYPYFGFRPATLDYALFKPNPGVFDNATGKHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKK+GYGDVDIVVAETGWPSAGDPNQ GVSMENAISYNRNLVK VNSG GTPLM
Sbjct: 241 DAVYSAMKKVGYGDVDIVVAETGWPSAGDPNQAGVSMENAISYNRNLVKHVNSGKGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETY+F+LFNENLKPS SERNYGLF+PD TPVYDVGI+ N P+MGPTSP    P+P
Sbjct: 301 PNRTFETYIFSLFNENLKPSTSERNYGLFQPDFTPVYDVGILQNPPAMGPTSP-VGGPKP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
               R+WCVPKTD SDEALQKNIDYVCSSGV+CGPI+ GGACY+PNTVRSHASYAMNAYF
Sbjct: 361 SDEEREWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGACYDPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKS----AGMTSRMIGAVALAS 480
           Q+AGRH+FNCDFN+TAILT+ DPSYE CSYPFDEEKLTEKS    A +  RM+GAVALA 
Sbjct: 421 QTAGRHEFNCDFNHTAILTSTDPSYEACSYPFDEEKLTEKSVASGATIRCRMVGAVALAR 480

Query: 481 PIVTAAAG 485
             +   AG
Sbjct: 481 ACIAVVAG 484

BLAST of CmoCh13G001240 vs. NCBI nr
Match: XP_022964795.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 960.7 bits (2482), Expect = 5.0e-276
Identity = 477/483 (98.76%), Postives = 477/483 (98.76%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE
Sbjct: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
           LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF
Sbjct: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVT 480
           QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALA   V 
Sbjct: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALACIAVV 480

Query: 481 AAA 484
             A
Sbjct: 481 GVA 483

BLAST of CmoCh13G001240 vs. NCBI nr
Match: KAG7019191.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 931.0 bits (2405), Expect = 4.2e-267
Identity = 461/475 (97.05%), Postives = 465/475 (97.89%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLFFRLTSAA SIGVNYGTVADNLPPP QVAAFLKSQTSIDRVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFFRLTSAANSIGVNYGTVADNLPPPSQVAAFLKSQTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANP+ILRAFAGTGIALTIS+VNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE
Sbjct: 61  DANPEILRAFAGTGIALTISIVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAHMLPAMKALHSALRLANIS IKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKNLIAHMLPAMKALHSALRLANISHIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGI+LNQPSMGPTSP A  PEP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGILLNQPSMGPTSPKAQPPEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
              GRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF
Sbjct: 361 SDAGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALA 476
           QSAGRHDFNCDFNNTA+LTAADPSYETCSYPFDEEKLTEKSA MTSRMIGAVALA
Sbjct: 421 QSAGRHDFNCDFNNTALLTAADPSYETCSYPFDEEKLTEKSAAMTSRMIGAVALA 475

BLAST of CmoCh13G001240 vs. NCBI nr
Match: XP_023519567.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 927.5 bits (2396), Expect = 4.7e-266
Identity = 466/499 (93.39%), Postives = 470/499 (94.19%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLFFRL SAAYSIGVNYGTVADNLPPP QVAAFLKSQTSIDRVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFFRLNSAAYSIGVNYGTVADNLPPPSQVAAFLKSQTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE
Sbjct: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGI+LNQPSMGPTSPNA APEP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGILLNQPSMGPTSPNAQAPEP 360

Query: 361 LYVGRKWCVPKTDVS----------------DEALQKNIDYVCSSGVECGPIEAGGACYN 420
             VGRKWCVPKTDVS                DEALQKNIDYVCSSGVECGPIEAGG+CYN
Sbjct: 361 SDVGRKWCVPKTDVSDEALQKNIDYVCSSGXDEALQKNIDYVCSSGVECGPIEAGGSCYN 420

Query: 421 PNTVRSHASYAMNAYFQSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGM 480
           PNTVRSHASYAMNAYFQSAGRHDFNCDFNNTA+L AADPSYETCSYPFDEEKLTEKSA +
Sbjct: 421 PNTVRSHASYAMNAYFQSAGRHDFNCDFNNTALLAAADPSYETCSYPFDEEKLTEKSAAI 480

Query: 481 TSRMIGAVALASPIVTAAA 484
           TSRMIGAVALA   V   A
Sbjct: 481 TSRMIGAVALACIAVVGVA 499

BLAST of CmoCh13G001240 vs. NCBI nr
Match: KAG6583428.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 918.3 bits (2372), Expect = 2.8e-263
Identity = 456/483 (94.41%), Postives = 464/483 (96.07%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLFFRL+SAA SIGVNYGTV+DNLPPP QVAAFLKSQTSIDRVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFFRLSSAANSIGVNYGTVSDNLPPPSQVAAFLKSQTSIDRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           +ANP+ILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWV INVLPFYPNTLINYIAVGNE
Sbjct: 61  EANPEILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVDINVLPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDKNLIAHMLPAMK LHSALRLANIS IKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKNLIAHMLPAMKELHSALRLANISHIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAP LEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPSLEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAIS+NRN+VKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISFNRNIVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGI+LNQPSMGPTSPNA  PEP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGILLNQPSMGPTSPNAQPPEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
              GRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGG CYNPNTVRSHASYAMNAYF
Sbjct: 361 SDAGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGECYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVT 480
           QSAGRHDFNCDFNNTA+LTAADPSYETCSYPFDEEKLTEKSA MTSRMIGAVALA   V 
Sbjct: 421 QSAGRHDFNCDFNNTALLTAADPSYETCSYPFDEEKLTEKSAAMTSRMIGAVALACIAVV 480

Query: 481 AAA 484
             A
Sbjct: 481 GVA 483

BLAST of CmoCh13G001240 vs. NCBI nr
Match: XP_022970527.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 914.8 bits (2363), Expect = 3.1e-262
Identity = 455/483 (94.20%), Postives = 462/483 (95.65%), Query Frame = 0

Query: 1   MASSPFFPLLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 60
           MASSPFFPLLLSLFSLF RLTSAAYSIGVNYGTVADNLPPP QVAAFLKSQTSI+RVKIF
Sbjct: 1   MASSPFFPLLLSLFSLFVRLTSAAYSIGVNYGTVADNLPPPSQVAAFLKSQTSINRVKIF 60

Query: 61  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 120
           DAN DILRAFAGTGIALTISV NGDIPTL+KLPAAQSWVAIN+LPFYPNTLINYIAVGNE
Sbjct: 61  DANQDILRAFAGTGIALTISVANGDIPTLSKLPAAQSWVAINILPFYPNTLINYIAVGNE 120

Query: 121 ILATSDKNLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180
           ILATSDK LIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR
Sbjct: 121 ILATSDKILIAHMLPAMKALHSALRLANISDIKVSTPHSLGILSASEPPSNGRFRRGYDR 180

Query: 181 VIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240
           VIFAPILEFHRRTKTPFMVNPYPFF FRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL
Sbjct: 181 VIFAPILEFHRRTKTPFMVNPYPFFGFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQL 240

Query: 241 DAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLM 300
           DAVYSAMKKLGY DVDIVVAETGWPSAGDPNQPGVS+ENAISYNRNLVKLVNSGIGTPLM
Sbjct: 241 DAVYSAMKKLGYEDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKLVNSGIGTPLM 300

Query: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEP 360
           PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGI+LNQPSMGPTSPNA A EP
Sbjct: 301 PNRTFETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGILLNQPSMGPTSPNAQASEP 360

Query: 361 LYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYF 420
             VGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIE+GGACYNPNTVRSHASYAMNAYF
Sbjct: 361 SDVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIESGGACYNPNTVRSHASYAMNAYF 420

Query: 421 QSAGRHDFNCDFNNTAILTAADPSYETCSYPFDEEKLTEKSAGMTSRMIGAVALASPIVT 480
           QSAGRHDFNCDFNNTA+LTA DPSYETCSYPFDEEKLTEKSA MT RMIGAVAL    V 
Sbjct: 421 QSAGRHDFNCDFNNTALLTATDPSYETCSYPFDEEKLTEKSAAMTCRMIGAVALTCFAVV 480

Query: 481 AAA 484
             A
Sbjct: 481 GVA 483

BLAST of CmoCh13G001240 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.1e-139
Identity = 242/454 (53.30%), Postives = 325/454 (71.59%), Query Frame = 0

Query: 5   PFFPLLLSLFS----LFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 64
           PFF   LS+F+    L    T +  SIGVNYGT+A+NLPPP QVA FL   T I+R+++F
Sbjct: 6   PFFS--LSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLF 65

Query: 65  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 124
           D +P IL+AFA TGIA+T++V N  IP L  L +A+ W++ ++ P +P+T I  I VGNE
Sbjct: 66  DTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNE 125

Query: 125 ILATSDKNLIAHMLPAMKALHSALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYD 184
           +++T+D  LI  ++PAM++LH+AL  A++   I++STPHSLG L+ S PPS+ +FRRGYD
Sbjct: 126 VISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYD 185

Query: 185 RVIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQ 244
             +  P+L F R T +PF+VNPYPFF +   TLDFALF+PN G+FD  T + YTNM DAQ
Sbjct: 186 AQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQ 245

Query: 245 LDAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPL 304
           LD+VYSAM KLG+ DV+IV+ E GWPS GD +Q GV ++ A  +N+NL+  V+SG GTPL
Sbjct: 246 LDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPL 305

Query: 305 MPNRTFETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPTSP--NAH 364
           MPNRTFETY+FALFNENLK    SERN+G+F+ DLTP+YD+GI+  +P+   + P  N  
Sbjct: 306 MPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGIL--RPTFRSSDPVYNPR 365

Query: 365 APEPLYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAM 424
           +P      ++WCV K      ALQ+NIDYVC  G++C PI  GG CY PNTV++H+ YAM
Sbjct: 366 SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAM 425

Query: 425 NAYFQSAGRHDFNCDFNNTAILTAADPSYETCSY 451
           N Y+Q+  +H+F+CDF+NT  +T  DPSY  C Y
Sbjct: 426 NLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of CmoCh13G001240 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 485.3 bits (1248), Expect = 5.7e-137
Identity = 239/448 (53.35%), Postives = 322/448 (71.88%), Query Frame = 0

Query: 5   PFFPLLLSLFS----LFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIF 64
           PFF   LS+F+    L    T +  SIGVNYGT+A+NLPPP QVA FL   T I+R+++F
Sbjct: 6   PFFS--LSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLF 65

Query: 65  DANPDILRAFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNE 124
           D +P IL+AFA TGIA+T++V N  IP L  L +A+ W++ ++ P +P+T I  I VGNE
Sbjct: 66  DTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNE 125

Query: 125 ILATSDKNLIAHMLPAMKALHSALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYD 184
           +++T+D  LI  ++PAM++LH+AL  A++   I++STPHSLG L+ S PPS+ +FRRGYD
Sbjct: 126 VISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYD 185

Query: 185 RVIFAPILEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVFDNVTGMHYTNMFDAQ 244
             +  P+L F R T +PF+VNPYPFF +   TLDFALF+PN G+FD  T + YTNM DAQ
Sbjct: 186 AQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQ 245

Query: 245 LDAVYSAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPL 304
           LD+VYSAM KLG+ DV+IV+ E GWPS GD +Q GV ++ A  +N+NL+  V+SG GTPL
Sbjct: 246 LDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPL 305

Query: 305 MPNRTFETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPTSP--NAH 364
           MPNRTFETY+FALFNENLK    SERN+G+F+ DLTP+YD+GI+  +P+   + P  N  
Sbjct: 306 MPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGIL--RPTFRSSDPVYNPR 365

Query: 365 APEPLYVGRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAM 424
           +P      ++WCV K      ALQ+NIDYVC  G++C PI  GG CY PNTV++H+ YAM
Sbjct: 366 SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAM 425

Query: 425 NAYFQSAGRHDFNCDFNNTAILTAADPS 445
           N Y+Q+  +H+F+CDF+NT  +T  DPS
Sbjct: 426 NLYYQTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of CmoCh13G001240 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.3e-133
Identity = 242/446 (54.26%), Postives = 316/446 (70.85%), Query Frame = 0

Query: 9   LLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILR 68
           LLL   ++F  + +   +IGVNYGT+  NLPPP QVA F+K+QTSID VKIFD NPDILR
Sbjct: 11  LLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNPDILR 70

Query: 69  AFAGTGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNEILATSDKN 128
           AFAGTGI++ ++V NGDIP LA    A+ WV++N+LPF+P T I YI+VGNEIL T D N
Sbjct: 71  AFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGDNN 130

Query: 129 LIAHMLPAMKALHSALRLANISDIKVSTPHSLGILS--ASEPPSNGRFRRGYDRVIFAPI 188
           +I ++LPAM+ L++AL  A + D+KV+T HSL I++   +  PS+GRFR G+D+ I API
Sbjct: 131 MINNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGILAPI 190

Query: 189 LEFHRRTKTPFMVNPYPFFSFRPATLDFALFK-PNAGVFDNVTGMHYTNMFDAQLDAVYS 248
           L +HRRTK+PFMVNPYP+F F P  ++FA+F+ P   V D  T   YTNMFDA +D+ YS
Sbjct: 191 LAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDSTYS 250

Query: 249 AMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRTF 308
           AMK LGYGDV+IVV ETGWPSA D   P  S  NA  +N N++K    G GTPLMPNR F
Sbjct: 251 AMKALGYGDVNIVVGETGWPSACD--APWCSPANAAWFNLNIIKRA-QGQGTPLMPNRRF 310

Query: 309 ETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEPLYVG 368
           ETY+F LFNE  KP   +ERN+GLF+ D +PVYDVG++ N    G        P P   G
Sbjct: 311 ETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLRNGQGGG---GRPALPAPSTAG 370

Query: 369 RKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYFQSAG 428
            KWCV ++  ++  LQ +I++VC  GV+C PI+AGG+C+NP+++R+HAS+ MNAYFQS G
Sbjct: 371 GKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVMNAYFQSHG 430

Query: 429 RHDFNCDFNNTAILTAADPSYETCSY 451
           R D  C+F+ T ++   +PS   C Y
Sbjct: 431 RTDGACNFSGTGMIVGNNPSNGACKY 449

BLAST of CmoCh13G001240 vs. TAIR 10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 4.1e-127
Identity = 229/337 (67.95%), Postives = 276/337 (81.90%), Query Frame = 0

Query: 9   LLLSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILR 68
           L+L+L   F    SAAY +GVNYGTVA+NLPPP QV  F+K++T I+ VKIFD N DIL 
Sbjct: 13  LILTLILPF----SAAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILA 72

Query: 69  AFAG-TGIALTISVVNGDIPTLAKLPAAQSWVAINVLPFYPNTLINYIAVGNEILATSDK 128
           AF+G TGI+LT++V N DI +L+KL  A+SW++ N+LPF   T I YIAVGNE++ATSDK
Sbjct: 73  AFSGITGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDK 132

Query: 129 NLIAHMLPAMKALHSALRLANISDIKVSTPHSLGILS-ASEPPSNGRFRRGYDRVIFAPI 188
            LI H+LPAM+ L  AL LAN+S I VSTPHSLGILS +SEPPS+G+FR+GYD+ IF+PI
Sbjct: 133 TLITHLLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPI 192

Query: 189 LEFHRRTKTPFMVNPYPFFSFRPATLDFALFKPNAGVF-DNVTGMHYTNMFDAQLDAVYS 248
           L+FH RTK+PFMVNPYP+F F P TL++ALF  N  V+ D VT ++YTNMFDAQLDAVYS
Sbjct: 193 LDFHNRTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYS 252

Query: 249 AMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRTF 308
           AMK+ GYGDVDIVVAETGWPSAG+PNQ GV ++ A +YN NL+K VNSG GTPLMPNR F
Sbjct: 253 AMKRFGYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVF 312

Query: 309 ETYVFALFNENLKPSASERNYGLFKPDLTPVYDVGII 343
           ETYVF+LFNENLK S SE+N+GLFKPD TPVYDVGI+
Sbjct: 313 ETYVFSLFNENLKSSVSEQNFGLFKPDFTPVYDVGIM 345

BLAST of CmoCh13G001240 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 432.2 bits (1110), Expect = 5.7e-121
Identity = 220/448 (49.11%), Postives = 302/448 (67.41%), Query Frame = 0

Query: 11  LSLFSLFFRLTSAAYSIGVNYGTVADNLPPPFQVAAFLKSQTSIDRVKIFDANPDILRAF 70
           L +F   F  + A   IGVNYG VADNLPPP +    L+S TSI +V+++ A+P I++A 
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKAL 69

Query: 71  AGTGIALTISVVNGDIPTLAKLP-AAQSWVAINVLPFYPNTLINYIAVGNEILATSDKNL 130
           AGTG+ + I   NGD+P+LA  P AA  W+  NVLPFYP + I  I VGNEIL ++D NL
Sbjct: 70  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNL 129

Query: 131 IAHMLPAMKALHSALRLANI-SDIKVSTPHSLGILSASEPPSNGRFRRGYDRVIFAPILE 190
           +  +LPAM+ +  AL   ++   IKVST +S+ +L +S+PPS+G F  GY   +   IL+
Sbjct: 130 VNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQ 189

Query: 191 FHRRTKTPFMVNPYPFFSF----RPATLDFALFKPNAGVFDNVTGMHYTNMFDAQLDAVY 250
           F   T +PF +NPYPFF++    RP TL F LF+PNAG  D+ TG+ YTNMFDAQ+DAV+
Sbjct: 190 FLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVH 249

Query: 251 SAMKKLGYGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKLVNSGIGTPLMPNRT 310
           SA+K +G+  V+IVVAETGW S GD N+ G S++NA +YN NL+  + S +GTPLMP + 
Sbjct: 250 SALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKP 309

Query: 311 FETYVFALFNENLKPS-ASERNYGLFKPDLTPVYDVGIILNQPSMGPTSPNAHAPEPLYV 370
            +TY+FAL++ENLKP  +SER +GLFK DL+ VYDVG       +  +S ++  P     
Sbjct: 310 VDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVG-------LAKSSSSSQTPSGKVT 369

Query: 371 GRKWCVPKTDVSDEALQKNIDYVCSSGVECGPIEAGGACYNPNTVRSHASYAMNAYFQSA 430
              WCVPK   ++E LQ ++D+ C  G++CG I+ GGAC+ PN V SHA+YAMN YFQ +
Sbjct: 370 SSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKS 429

Query: 431 GRHDFNCDFNNTAILTAADPSYETCSYP 452
            +   +CDF+ TA +T+ +PSY  C YP
Sbjct: 430 PKQPTDCDFSKTATVTSQNPSYNNCVYP 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524092.0e-13150.54Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0698.1e-12049.11Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G864.9e-11747.33Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE59.0e-8738.90Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q8L8681.0e-8249.00Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Match NameE-valueIdentityDescription
A0A6J1HLU32.4e-27698.76(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111464794 P... [more]
A0A6J1HZC81.5e-26294.20(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111469479 PE=... [more]
A0A5D3BDL61.2e-23082.27(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C6X21.2e-23082.27(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497159 PE=3 SV... [more]
A0A0A0LVC14.4e-23081.97(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3... [more]
Match NameE-valueIdentityDescription
XP_022964795.15.0e-27698.76glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata][more]
KAG7019191.14.2e-26797.05Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_023519567.14.7e-26693.39glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
KAG6583428.12.8e-26394.41Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022970527.13.1e-26294.20glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G24318.12.1e-13953.30O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.25.7e-13753.35O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.11.3e-13354.26O-Glycosyl hydrolases family 17 protein [more]
AT3G46570.14.1e-12767.95Glycosyl hydrolase superfamily protein [more]
AT4G34480.15.7e-12149.11O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 366..450
e-value: 5.0E-46
score: 168.9
IPR012946X8 domainPFAMPF07983X8coord: 366..436
e-value: 6.7E-22
score: 78.0
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 27..339
e-value: 8.3E-84
score: 281.8
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 255..268
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..345
e-value: 1.4E-116
score: 391.0
NoneNo IPR availableGENE3D1.20.58.1040coord: 363..463
e-value: 3.0E-12
score: 48.8
NoneNo IPR availablePANTHERPTHR32227:SF359GLUCOSIDASE-LIKE PROTEINcoord: 13..456
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 13..456
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 27..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G001240.1CmoCh13G001240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds