CmoCh12G013370 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G013370
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCytokinin dehydrogenase
LocationCmo_Chr12: 11609815 .. 11613714 (-)
RNA-Seq ExpressionCmoCh12G013370
SyntenyCmoCh12G013370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACCCTTTATTCTTCTTTCCTTTGAAAAACCACCATGACGGAAAATCTCCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCCTACACCCGCAACCAAACAACCTTGTACACGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCCAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATTAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGGTATGGAAAACGAAACGACGTGTAATTTTGTATTTAAATATAAATATAAATAAAAAATAATTTATTTGTTCATACGATATTAAAAGGACAAGTTTGGTTGTTACATAATGTGTATTAAGTACAGACATTTTTCTACATATCATTAAATGATATGACAAAGCTGTTTATTTTGTGGTCCCTGAGTTTGAACTCTAAAAAAAAATGTATTTTTCAAACTAGGTGTTTCCAGTCTATTTTGTACAAATAGACAGCTTGTCTTTTAAAAATAAATTATTATTGCATGCATGCAATTAATGATAACATGACTATCGGTATGATATTGTCCGACTTCAAGCATAAGCTCTTATGGCTTTGCTTAGGACATACTAAAAAAGGATTCATCATACCCATGAATATAGTATTCTTTGATTATAAACTCATGATCATTTTTTAAATTTGCAGGTAAGGGGAATATGGTGACTTGCTCTGCTAAAAAGAATTCTGAGCTATTCCATGCGGTCCTTGGAGGTTTAGGTCAATTTGGGATTATTGCTCGGGCAAGAATAGCTTTGGAACCAGCTCCAAAGAGGGTAATTTTTATTTATTTTCTTTTTTTTATATATATTTATTTTATGGTTTTTTTTTAATATTTAAAAGGCTTATTCTTATAATTATGGTTTATATTTCTTTTATATATATATATATATAGTAAGGTTTTATATAGTTTGTAAGGTTGATTAAAAGTAAGGAAGAATCTATGGATGCTTCTAAACCACCCAACATATCATCGTCTTTGTTTGGGAGTGTTTCTGTCATTTCCAACCATCAAAACGGTGTCGTATCTAATAATACGAACATATTCCAAATTAATTCTCATGCATATGTTTTACTATTTCTTAATCTCATGCTTTGTCTGCTAATTATGATTATTATTAGTACTATTATGTACGCACGCATGTGAAATTCGAAGATACTGATGATGATGTGGAATTCGGTCGCAGGTTAAATGGGTACGAATGTTGTACACTAATTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGAAAACAAGCGAATGCGCTGGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAGTAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCATCATACTCAGTCTACTATAGACAAGGTAAAAATTTAATACTTTTCTCTCTCCCGAGGTCTAAAAAAAGATAATTCTTATATGATTGTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTTTTTTTTTTTTTTTTTTTTTTTGTAGGATCTTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGGGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGCAGGTGTCTTCAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTAAGCTTGTAGAATAACGTTTTCTATACCTAACGTATATCGATTGATCGTAATTAATATTATGAATTGGTACGATAAAAATGCAGGTGGGACAGTAGAAATTCGACGGTTATACCCAACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATCACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGATGTTATGTTTTTCCCCTTGCAGTGTAAGAAAACAAATACTATATATATAGGTAGGAGGCAGTAATTAGCTTTTTAACTAATATTTTTT

mRNA sequence

AAAACCCTTTATTCTTCTTTCCTTTGAAAAACCACCATGACGGAAAATCTCCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCCTACACCCGCAACCAAACAACCTTGTACACGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCCAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATTAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGGTAAGGGGAATATGGTGACTTGCTCTGCTAAAAAGAATTCTGAGCTATTCCATGCGGTCCTTGGAGGTTTAGGTCAATTTGGGATTATTGCTCGGGCAAGAATAGCTTTGGAACCAGCTCCAAAGAGGGTTAAATGGGTACGAATGTTGTACACTAATTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGAAAACAAGCGAATGCGCTGGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAGTAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCATCATACTCAGTCTACTATAGACAAGGATCTTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGGGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGCAGGTGTCTTCAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTGGGACAGTAGAAATTCGACGGTTATACCCAACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATCACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGATGTTATGTTTTTCCCCTTGCAGTGTAAGAAAACAAATACTATATATATAGGTAGGAGGCAGTAATTAGCTTTTTAACTAATATTTTTT

Coding sequence (CDS)

ATGACGGAAAATCTCCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCCTACACCCGCAACCAAACAACCTTGTACACGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCCAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATTAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGGTAAGGGGAATATGGTGACTTGCTCTGCTAAAAAGAATTCTGAGCTATTCCATGCGGTCCTTGGAGGTTTAGGTCAATTTGGGATTATTGCTCGGGCAAGAATAGCTTTGGAACCAGCTCCAAAGAGGGTTAAATGGGTACGAATGTTGTACACTAATTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGAAAACAAGCGAATGCGCTGGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAGTAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCATCATACTCAGTCTACTATAGACAAGGATCTTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGGGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGCAGGTGTCTTCAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTGGGACAGTAGAAATTCGACGGTTATACCCAACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATCACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGA

Protein sequence

MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV
Homology
BLAST of CmoCh12G013370 vs. ExPASy Swiss-Prot
Match: Q9LTS3 (Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.9e-167
Identity = 278/496 (56.05%), Postives = 369/496 (74.40%), Query Frame = 0

Query: 26  KPW-ITLHPQ-PNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNP 85
           +PW I  H +    L     S+  A++D+G++ K  P+AVL P+S+ D+  LI  S+++ 
Sbjct: 34  QPWNILSHNEFAGKLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQ 93

Query: 86  IPFPVAARGQGHSVRGQAMAKNGVVIDM-SYLRRNRETPGIIISRLRSTGEIYADVGGEQ 145
           + FP+AARG GHS RGQA AK+GVV++M S + R+R   GI +SR      +Y DV    
Sbjct: 94  LSFPLAARGHGHSHRGQASAKDGVVVNMRSMVNRDR---GIKVSRTC----LYVDVDAAW 153

Query: 146 LWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKG 205
           LWI+VLN  L  G+TPVSWTDYLY+TVGGTLSNGGISGQ+FRYGPQ++NV+EMDV+TGKG
Sbjct: 154 LWIEVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKG 213

Query: 206 NMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQER 265
            + TCS   NS+LF AVLGGLGQFGII RARI LE APKR KW+R LY +FS F +DQER
Sbjct: 214 EIATCSKDMNSDLFFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQER 273

Query: 266 LISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAK 325
           +IS     + + +D+LEG +++ H  PD WRS+++PPSDH R+ S+  ++ +IYCLEV K
Sbjct: 274 VIS-----KTDGVDFLEGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVK 333

Query: 326 YYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHP 385
           YYD  +Q T+++++E L + L++  GF +EKDVTY+ FLNRVR+GEL L+S+G W+VPHP
Sbjct: 334 YYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHP 393

Query: 386 WLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTI 445
           WLNLFVPK++I  FD GVFK I+LR NIT GPVL+YPMN +KW+ R S  IP E+VFY +
Sbjct: 394 WLNLFVPKTQISKFDDGVFKGIILRNNITSGPVLVYPMNRNKWNDRMSAAIPEEDVFYAV 453

Query: 446 GFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMK 505
           GFL S+GFD+W++FD++N EIL+FCE + + + QYLP H +Q  W+ HFG +W  F + K
Sbjct: 454 GFLRSAGFDNWEAFDQENMEILKFCEDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERK 513

Query: 506 MKFDPKYILSPGQRIF 519
            K+DPK ILSPGQ IF
Sbjct: 514 YKYDPKMILSPGQNIF 517

BLAST of CmoCh12G013370 vs. ExPASy Swiss-Prot
Match: Q9FUJ2 (Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2)

HSP 1 Score: 512.3 bits (1318), Expect = 6.4e-144
Identity = 264/523 (50.48%), Postives = 346/523 (66.16%), Query Frame = 0

Query: 10  IVTFFINRLKSSINKSKPWITLHPQPNNL--VHDPESLTLASSDYGNMVKEIPAAVLQPT 69
           ++T FI+   + I   +      P   NL  + DP S++ AS D+GN+  E P AVL P+
Sbjct: 12  LITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPS 71

Query: 70  SINDVVHLI-----NYSYNN-----PIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 129
           S  +V  L+      +SYN         F VAARGQGHS+RGQA A  GVV++M+ L   
Sbjct: 72  STTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVNMTCLAMA 131

Query: 130 RETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGG 189
            +   ++I    S    YADV    +W+DVL AA+  GV+PV+WTDYLY++VGGTLSN G
Sbjct: 132 AKPAAVVI----SADGTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAG 191

Query: 190 ISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALE 249
           I GQ+FR+GPQ+SNV E+DV+TGKG M+TCS K N ELF+ VLGGLGQFGII RARIAL+
Sbjct: 192 IGGQTFRHGPQISNVHELDVITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALD 251

Query: 250 PAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFF 309
            AP RVKW R+LY++FSAF +DQERLIS         +D+LEG +++ +   DT   SFF
Sbjct: 252 HAPTRVKWSRILYSDFSAFKRDQERLISMTNDL---GVDFLEGQLMMSNGFVDT---SFF 311

Query: 310 PPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTY 369
           P SD  RV SL N + +IY LEVAKYYD  T   ID+ ++ L   L +  GF F +DV Y
Sbjct: 312 PLSDQTRVASLVNDHRIIYVLEVAKYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPY 371

Query: 370 VKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLI 429
             FLNRVR+ E KL+S GLW VPHPWLN+FVP SRI DF  GV   ++L +  T G  L 
Sbjct: 372 FDFLNRVRNEEDKLRSLGLWEVPHPWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLF 431

Query: 430 YPMNHSKWDSRNSTVIPNEEVFYTIGFLHSS-GFDDWKSFDRQNDEILEFCEKSGIEIKQ 489
           YP N +KW++R ST+ P+E+VFY IG L S+ G  +W+  +  ND++++FCE SGI+IK+
Sbjct: 432 YPTNRNKWNNRMSTMTPDEDVFYVIGLLQSAGGSQNWQELENLNDKVIQFCENSGIKIKE 491

Query: 490 YLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 520
           YL ++  +  W+ HFG KW +F   K+ FDPK +LSPGQ IFN
Sbjct: 492 YLMHYTRKEDWVKHFGPKWDDFLRKKIMFDPKRLLSPGQDIFN 524

BLAST of CmoCh12G013370 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.4e-144
Identity = 248/484 (51.24%), Postives = 332/484 (68.60%), Query Frame = 0

Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 48  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 107

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 108 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 167

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAV 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVTGKG ++ CS ++N+ LFH V
Sbjct: 168 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGV 227

Query: 222 LGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLE 281
           LGGLGQFGII RARI+LEPAP+RV+W+R+LY++F  F +DQE LIS + + +    DY+E
Sbjct: 228 LGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYVE 287

Query: 282 GLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVL 341
           G V++     + WRSSFF P +  ++ S+++  S++YCLE+ K Y       +D+++E+L
Sbjct: 288 GFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEIL 347

Query: 342 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAG 401
            + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD G
Sbjct: 348 MKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKG 407

Query: 402 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKS---- 461
           VFK I+   N T GP+LIYPMN  KWD R+S V P+EEVFY +  L S+  D  ++    
Sbjct: 408 VFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLE 467

Query: 462 -FDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPG 519
               QN  ILEFCE++ I +KQYLP+H TQ +W+ HFG KW  FR +K +FDP++IL+ G
Sbjct: 468 YLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATG 520

BLAST of CmoCh12G013370 vs. ExPASy Swiss-Prot
Match: Q5ZAY9 (Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.5e-140
Identity = 249/486 (51.23%), Postives = 319/486 (65.64%), Query Frame = 0

Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           +P  +  AS D+G +    P AV  P    DV  L+  +Y +     V+ARG GHS+ GQ
Sbjct: 44  EPSDVMEASLDFGRLTSAEPLAVFHPRGAGDVAALVKAAYGSASGIRVSARGHGHSISGQ 103

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAY-GVTPV 160
           A A  GVV+DMS+  R       +     + G  Y DV G +LWIDVLN  LA+ G+ P 
Sbjct: 104 AQAAGGVVVDMSHGWRAEAAERTLPVYSPALGGHYIDVWGGELWIDVLNWTLAHGGLAPR 163

Query: 161 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAV 220
           SWTDYLY++VGGTLSN GISGQ+F +GPQ+SNV E+DVVTGKG +VTCS   N +LF   
Sbjct: 164 SWTDYLYLSVGGTLSNAGISGQAFHHGPQISNVYELDVVTGKGEVVTCSESNNPDLFFGA 223

Query: 221 LGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLE 280
           LGGLGQ GII RARIALEPAP RV+W+R LY+NF+ F  DQERLIS   +      DY+E
Sbjct: 224 LGGLGQLGIITRARIALEPAPHRVRWIRALYSNFTEFTADQERLISL--QHGGRRFDYVE 283

Query: 281 GLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVL 340
           G V+      + WRSSFF P +  ++ SL + + ++YCLEV K YD  T  T+D+D+E L
Sbjct: 284 GFVVAAEGLINNWRSSFFSPQNPVKLSSLKHHSGVLYCLEVTKNYDDSTAVTVDQDVEAL 343

Query: 341 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAG 400
              L++  G  F  D+ YV FL+RV   ELKL+ +G+W VPHPWLNLFVP SRI DFD G
Sbjct: 344 LGELNFIPGTVFTTDLPYVDFLDRVHKAELKLRGKGMWEVPHPWLNLFVPASRIADFDRG 403

Query: 401 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSS--GFDD----W 460
           VF+  VL      GP+LIYPMN  KWD R+S V P E+VFY + FL S+  G  D     
Sbjct: 404 VFRG-VLGSRTAGGPILIYPMNRHKWDPRSSVVTPEEDVFYLVAFLRSAVPGSTDPAQSL 463

Query: 461 KSFDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSP 520
           ++ +RQN EILEFC+++GI  KQYLPNHK QR+W  HFG++W  F  +K +FDP+ +L+ 
Sbjct: 464 EALERQNREILEFCDEAGIGAKQYLPNHKAQREWEAHFGARWARFARLKAEFDPRAMLAT 523

BLAST of CmoCh12G013370 vs. ExPASy Swiss-Prot
Match: Q9FUJ3 (Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2)

HSP 1 Score: 492.3 bits (1266), Expect = 6.9e-138
Identity = 251/482 (52.07%), Postives = 319/482 (66.18%), Query Frame = 0

Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           DP  ++ AS D+GN+    P  V+ P+S  D+  L+ Y+ N    F VAARGQGHS+ GQ
Sbjct: 38  DPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQVAARGQGHSLNGQ 97

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVS 160
           A    GV+++M+ +        +++S+ +     YADV    LW+DVL      GV+PVS
Sbjct: 98  ASVSGGVIVNMTCI------TDVVVSKDKK----YADVAAGTLWVDVLKKTAEKGVSPVS 157

Query: 161 WTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVL 220
           WTDYL+ITVGGTLSNGGI GQ FR GP VSNV+E+DV+TGKG M+TCS + N ELF+ VL
Sbjct: 158 WTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLTCSRQLNPELFYGVL 217

Query: 221 GGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQAN--ALDYL 280
           GGLGQFGII RARI L+ APKR KW RMLY++F+ F KDQERLIS      AN   +DYL
Sbjct: 218 GGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLIS-----MANDIGVDYL 277

Query: 281 EGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEV 340
           EG + L +   DT   SFFPPSD  +V  L  Q+ +IY LEVAKYYD      I K ++ 
Sbjct: 278 EGQIFLSNGVVDT---SFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPIISKVIDT 337

Query: 341 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDA 400
           L + L Y  GF    DV Y  FLNRV   E KL+S GLW +PHPWLNL+VPKSRILDF  
Sbjct: 338 LTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKSRILDFHN 397

Query: 401 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIP--NEEVFYTIGFLHSSGFDDWKSF 460
           GV KDI+L++    G  L+YP N +KWD+R S +IP  +E+V Y IG L S+   D    
Sbjct: 398 GVVKDILLKQKSASGLALLYPTNRNKWDNRMSAMIPEIDEDVIYIIGLLQSATPKDLPEV 457

Query: 461 DRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQR 519
           +  N++I+ FC+ SGI+IKQYL ++ ++  WI+HFGSKW +F   K  FDPK +LSPGQ 
Sbjct: 458 ESVNEKIIRFCKDSGIKIKQYLMHYTSKEDWIEHFGSKWDDFSKRKDLFDPKKLLSPGQD 501

BLAST of CmoCh12G013370 vs. ExPASy TrEMBL
Match: A0A6J1FD08 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111444271 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP 240
           QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP
Sbjct: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP 240

Query: 241 KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
           KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN
Sbjct: 421 NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480

Query: 481 HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 521
           HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV
Sbjct: 481 HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 520

BLAST of CmoCh12G013370 vs. ExPASy TrEMBL
Match: A0A6J1HKZ4 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111465505 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.3e-301
Identity = 500/520 (96.15%), Postives = 512/520 (98.46%), Query Frame = 0

Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSI+KSKPWITLHPQPNNLV DPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSISKSKPWITLHPQPNNLVDDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGI ISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGITISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP 240
           Q+FRYGPQVSNVVEMDVVTG+GNM+TCSAKKNSELFHAVLGGLGQFGII+RARIALEPAP
Sbjct: 181 QTFRYGPQVSNVVEMDVVTGQGNMLTCSAKKNSELFHAVLGGLGQFGIISRARIALEPAP 240

Query: 241 KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
            RVKW+RMLYTNF AFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 NRVKWIRMLYTNFFAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPR+ISLANQNS+IYCLEVAKYYD HTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRIISLANQNSVIYCLEVAKYYDRHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQ LWNVPHPWLNLFVPKSRI DFDAGVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQALWNVPHPWLNLFVPKSRIFDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           N+SKWDSRNSTVIP+EEVFYTIGFLHSSGFDDWKSFDRQN+EILEFCEKSGIEIKQYLPN
Sbjct: 421 NYSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNEEILEFCEKSGIEIKQYLPN 480

Query: 481 HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 521
           +KTQRQWIDHFGSKWTNF DMKMKFDPKYILSPGQRIFNV
Sbjct: 481 YKTQRQWIDHFGSKWTNFGDMKMKFDPKYILSPGQRIFNV 520

BLAST of CmoCh12G013370 vs. ExPASy TrEMBL
Match: A0A1S3C9Z6 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498521 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.6e-262
Identity = 436/523 (83.37%), Postives = 479/523 (91.59%), Query Frame = 0

Query: 1   MTENLPV--HFIVTFFINRLKSSINKSKPW-ITLHPQPNNLVHDPESLTLASSDYGNMVK 60
           M ENLPV  +FI+TFFI+RLKSSINKSK W  TL+ +PNNL++DPE+LTLASSDYGNMVK
Sbjct: 1   MNENLPVPAYFIITFFISRLKSSINKSKAWTTTLNQRPNNLLNDPETLTLASSDYGNMVK 60

Query: 61  EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 120
           E PAAVLQP+SIND+V LI+Y+YNNPIPF +AARGQGHSVRGQAMAKNGVVIDMS LRRN
Sbjct: 61  ETPAAVLQPSSINDIVQLISYAYNNPIPFQIAARGQGHSVRGQAMAKNGVVIDMSALRRN 120

Query: 121 RETPGIIIS-RLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNG 180
           R+TPGI++S R  +TGE Y DVGGEQLWIDVLN  L YG+TPVSWTDYLYITVGGTLSN 
Sbjct: 121 RKTPGIVVSCRRWTTGEFYVDVGGEQLWIDVLNETLGYGMTPVSWTDYLYITVGGTLSNA 180

Query: 181 GISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIAL 240
           GISGQSFRYGPQVSNVVEMDVVTGKGNM+TCS KKN ELFHAVLGGLGQFGIIARARIAL
Sbjct: 181 GISGQSFRYGPQVSNVVEMDVVTGKGNMMTCSPKKNCELFHAVLGGLGQFGIIARARIAL 240

Query: 241 EPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSF 300
           EPAP RVKWVRMLYTNF+AF KDQE LIS N RKQ NALDYLEGLVLLHH+ PD WRSSF
Sbjct: 241 EPAPIRVKWVRMLYTNFAAFTKDQEHLISLNGRKQMNALDYLEGLVLLHHNKPDNWRSSF 300

Query: 301 FPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVT 360
           FPPSDH R+ISLANQNS+IYCLEV KYYDHHTQST+DKDLEVL +GLDYESGFKFEKDVT
Sbjct: 301 FPPSDHSRIISLANQNSIIYCLEVVKYYDHHTQSTVDKDLEVLLQGLDYESGFKFEKDVT 360

Query: 361 YVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVL 420
           YV+FLNRVR+GELKLQS+GLW+VPHPWLNLFVP+SRI+DF++GVFKDIVLRRNITKGP+L
Sbjct: 361 YVEFLNRVRTGELKLQSKGLWDVPHPWLNLFVPRSRIMDFNSGVFKDIVLRRNITKGPIL 420

Query: 421 IYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQ 480
           IYPMN SKWD RNSTVIP+EEVFYTIGFL+SSGFDDWK F+ QN+EILE+C KSGIEIKQ
Sbjct: 421 IYPMNRSKWDERNSTVIPDEEVFYTIGFLNSSGFDDWKKFEEQNEEILEYCGKSGIEIKQ 480

Query: 481 YLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 520
           YLP++KTQ QWI HFGSKWTNF+  K KFDPK ILSPGQ+IFN
Sbjct: 481 YLPHYKTQTQWIQHFGSKWTNFQHNKFKFDPKNILSPGQKIFN 523

BLAST of CmoCh12G013370 vs. ExPASy TrEMBL
Match: A0A6J1G4H4 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111450699 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 7.9e-262
Identity = 435/522 (83.33%), Postives = 473/522 (90.61%), Query Frame = 0

Query: 1   MTEN--LPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKE 60
           MTEN  LP +FI+TFF++ LKS INKSKPW  LH QPN L HDPE+L LA+SDYGNMVKE
Sbjct: 1   MTENHPLPAYFILTFFLSCLKSGINKSKPWTGLHQQPNKLRHDPEALNLAASDYGNMVKE 60

Query: 61  IPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNR 120
            PAAVLQP+SINDVVHLI+Y+YNNPIPFP+AARGQGHSVRGQAMAKNGVVIDMS LRRNR
Sbjct: 61  APAAVLQPSSINDVVHLISYAYNNPIPFPIAARGQGHSVRGQAMAKNGVVIDMSALRRNR 120

Query: 121 ETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGI 180
           +T GI +   R TGE+YADVGGEQLWIDVLNA LAYGVTPVSWTDYLYITVGGTLSNGGI
Sbjct: 121 KTAGIAVFARRWTGELYADVGGEQLWIDVLNATLAYGVTPVSWTDYLYITVGGTLSNGGI 180

Query: 181 SGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEP 240
           SGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAK+NSELFHAVLGGLGQFGIIA+ARIALEP
Sbjct: 181 SGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKRNSELFHAVLGGLGQFGIIAQARIALEP 240

Query: 241 APKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFP 300
           AP RVKWVRMLY+NFSAF KDQERLIS N RKQA+ALDYLEGLVLLHH SPD+WRSSFFP
Sbjct: 241 APNRVKWVRMLYSNFSAFTKDQERLISLNGRKQASALDYLEGLVLLHHSSPDSWRSSFFP 300

Query: 301 PSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYV 360
           PSDHPR+IS  NQ S++YCLEVAKYYDHHT ST+DK+LEVL   LDYESGFKFEKDVTYV
Sbjct: 301 PSDHPRIISFVNQYSVVYCLEVAKYYDHHTLSTVDKELEVLLAELDYESGFKFEKDVTYV 360

Query: 361 KFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIY 420
           +FLNRVR+GELKLQS+GLW+V HPWLNLFVPKSRILDFD+GVFKDIVLRRNITKGPVLIY
Sbjct: 361 EFLNRVRTGELKLQSKGLWDVSHPWLNLFVPKSRILDFDSGVFKDIVLRRNITKGPVLIY 420

Query: 421 PMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYL 480
           PMN SKWD RNSTVIP EEVFYTIGFLHSSGFD+ K FD QN+EI+EFC KSGI+IKQYL
Sbjct: 421 PMNRSKWDDRNSTVIPEEEVFYTIGFLHSSGFDERKLFDEQNEEIIEFCTKSGIQIKQYL 480

Query: 481 PNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 521
           P++ TQ QWI HFGSKW  FR  K+K+DPK ILSPG +IFN+
Sbjct: 481 PHYXTQTQWIHHFGSKWPTFRQRKIKYDPKLILSPGHKIFNL 522

BLAST of CmoCh12G013370 vs. ExPASy TrEMBL
Match: A0A6J1DPL8 (Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021923 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.8e-253
Identity = 426/523 (81.45%), Postives = 465/523 (88.91%), Query Frame = 0

Query: 1   MTENLPV--HFIVTFFINRLKSSINKSKPWITLHPQPN--NLVHDPESLTLASSDYGNMV 60
           MTENLPV  +FIVTFFI+RLKSS+ KSKPW + H QPN   L  DPE+L  AS+DYGNMV
Sbjct: 1   MTENLPVPAYFIVTFFISRLKSSMGKSKPWTSFHLQPNKLRLRDDPEALNSASTDYGNMV 60

Query: 61  KEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRR 120
           KE PAAVL P+SINDVV LI+Y+YNNPIPF +AARGQGHSVRGQAMA++GVVIDMS LRR
Sbjct: 61  KETPAAVLNPSSINDVVQLISYAYNNPIPFQIAARGQGHSVRGQAMARDGVVIDMSSLRR 120

Query: 121 NRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNG 180
           NR TPGI +    STGE YADVGGEQ WIDVLNA L YG+TPVSWTDYLYITVGGTLSN 
Sbjct: 121 NRATPGITV----STGEFYADVGGEQRWIDVLNATLEYGLTPVSWTDYLYITVGGTLSNA 180

Query: 181 GISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIAL 240
           GISGQ+FRYGPQVSNVVEMDVVTGKGNMVTCSAK++SELFHAVLGGLGQFGIIARARI L
Sbjct: 181 GISGQTFRYGPQVSNVVEMDVVTGKGNMVTCSAKRDSELFHAVLGGLGQFGIIARARIVL 240

Query: 241 EPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSF 300
           E AP RVKWVRMLY+NFS FAKDQE LIS + RKQ NALDYLEGLVLLHH SPD WRSSF
Sbjct: 241 ERAPTRVKWVRMLYSNFSEFAKDQENLISLHGRKQENALDYLEGLVLLHHGSPDNWRSSF 300

Query: 301 FPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVT 360
           FPPSDHPR+I+LANQNSLIYCLEV KYYD  TQS +DK+++ L EGL YE GFKFEKDV+
Sbjct: 301 FPPSDHPRIIALANQNSLIYCLEVVKYYDDSTQSIVDKEVDALLEGLGYERGFKFEKDVS 360

Query: 361 YVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVL 420
           YV+FLNRVRSGELKLQSQGLW+VPHPWLNLF+PKSRI DFD+GVFKDIVLR NITKGPVL
Sbjct: 361 YVEFLNRVRSGELKLQSQGLWDVPHPWLNLFIPKSRISDFDSGVFKDIVLRHNITKGPVL 420

Query: 421 IYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQ 480
           IYPMN SKWDSRNSTVIP+EEVFYTIGFLHSSGF DWKSFD QN+EILEFC KSGIEIKQ
Sbjct: 421 IYPMNRSKWDSRNSTVIPDEEVFYTIGFLHSSGFGDWKSFDEQNEEILEFCAKSGIEIKQ 480

Query: 481 YLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 520
           YLP+H T++QWI HFGSKWT F++ KMKFDPK+ILSPG +IFN
Sbjct: 481 YLPHHNTKQQWIHHFGSKWTTFQERKMKFDPKHILSPGHKIFN 519

BLAST of CmoCh12G013370 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-168
Identity = 278/496 (56.05%), Postives = 369/496 (74.40%), Query Frame = 0

Query: 26  KPW-ITLHPQ-PNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNP 85
           +PW I  H +    L     S+  A++D+G++ K  P+AVL P+S+ D+  LI  S+++ 
Sbjct: 34  QPWNILSHNEFAGKLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQ 93

Query: 86  IPFPVAARGQGHSVRGQAMAKNGVVIDM-SYLRRNRETPGIIISRLRSTGEIYADVGGEQ 145
           + FP+AARG GHS RGQA AK+GVV++M S + R+R   GI +SR      +Y DV    
Sbjct: 94  LSFPLAARGHGHSHRGQASAKDGVVVNMRSMVNRDR---GIKVSRTC----LYVDVDAAW 153

Query: 146 LWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKG 205
           LWI+VLN  L  G+TPVSWTDYLY+TVGGTLSNGGISGQ+FRYGPQ++NV+EMDV+TGKG
Sbjct: 154 LWIEVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKG 213

Query: 206 NMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQER 265
            + TCS   NS+LF AVLGGLGQFGII RARI LE APKR KW+R LY +FS F +DQER
Sbjct: 214 EIATCSKDMNSDLFFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQER 273

Query: 266 LISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAK 325
           +IS     + + +D+LEG +++ H  PD WRS+++PPSDH R+ S+  ++ +IYCLEV K
Sbjct: 274 VIS-----KTDGVDFLEGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVK 333

Query: 326 YYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHP 385
           YYD  +Q T+++++E L + L++  GF +EKDVTY+ FLNRVR+GEL L+S+G W+VPHP
Sbjct: 334 YYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHP 393

Query: 386 WLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTI 445
           WLNLFVPK++I  FD GVFK I+LR NIT GPVL+YPMN +KW+ R S  IP E+VFY +
Sbjct: 394 WLNLFVPKTQISKFDDGVFKGIILRNNITSGPVLVYPMNRNKWNDRMSAAIPEEDVFYAV 453

Query: 446 GFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMK 505
           GFL S+GFD+W++FD++N EIL+FCE + + + QYLP H +Q  W+ HFG +W  F + K
Sbjct: 454 GFLRSAGFDNWEAFDQENMEILKFCEDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERK 513

Query: 506 MKFDPKYILSPGQRIF 519
            K+DPK ILSPGQ IF
Sbjct: 514 YKYDPKMILSPGQNIF 517

BLAST of CmoCh12G013370 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 512.3 bits (1318), Expect = 4.6e-145
Identity = 248/484 (51.24%), Postives = 332/484 (68.60%), Query Frame = 0

Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 48  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 107

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 108 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 167

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAV 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVTGKG ++ CS ++N+ LFH V
Sbjct: 168 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGV 227

Query: 222 LGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLE 281
           LGGLGQFGII RARI+LEPAP+RV+W+R+LY++F  F +DQE LIS + + +    DY+E
Sbjct: 228 LGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYVE 287

Query: 282 GLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVL 341
           G V++     + WRSSFF P +  ++ S+++  S++YCLE+ K Y       +D+++E+L
Sbjct: 288 GFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEIL 347

Query: 342 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAG 401
            + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD G
Sbjct: 348 MKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKG 407

Query: 402 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKS---- 461
           VFK I+   N T GP+LIYPMN  KWD R+S V P+EEVFY +  L S+  D  ++    
Sbjct: 408 VFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLE 467

Query: 462 -FDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPG 519
               QN  ILEFCE++ I +KQYLP+H TQ +W+ HFG KW  FR +K +FDP++IL+ G
Sbjct: 468 YLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATG 520

BLAST of CmoCh12G013370 vs. TAIR 10
Match: AT4G29740.2 (cytokinin oxidase 4 )

HSP 1 Score: 512.3 bits (1318), Expect = 4.6e-145
Identity = 264/523 (50.48%), Postives = 346/523 (66.16%), Query Frame = 0

Query: 10  IVTFFINRLKSSINKSKPWITLHPQPNNL--VHDPESLTLASSDYGNMVKEIPAAVLQPT 69
           ++T FI+   + I   +      P   NL  + DP S++ AS D+GN+  E P AVL P+
Sbjct: 12  LITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPS 71

Query: 70  SINDVVHLI-----NYSYNN-----PIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 129
           S  +V  L+      +SYN         F VAARGQGHS+RGQA A  GVV++M+ L   
Sbjct: 72  STTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVNMTCLAMA 131

Query: 130 RETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGG 189
            +   ++I    S    YADV    +W+DVL AA+  GV+PV+WTDYLY++VGGTLSN G
Sbjct: 132 AKPAAVVI----SADGTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAG 191

Query: 190 ISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALE 249
           I GQ+FR+GPQ+SNV E+DV+TGKG M+TCS K N ELF+ VLGGLGQFGII RARIAL+
Sbjct: 192 IGGQTFRHGPQISNVHELDVITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALD 251

Query: 250 PAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFF 309
            AP RVKW R+LY++FSAF +DQERLIS         +D+LEG +++ +   DT   SFF
Sbjct: 252 HAPTRVKWSRILYSDFSAFKRDQERLISMTNDL---GVDFLEGQLMMSNGFVDT---SFF 311

Query: 310 PPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTY 369
           P SD  RV SL N + +IY LEVAKYYD  T   ID+ ++ L   L +  GF F +DV Y
Sbjct: 312 PLSDQTRVASLVNDHRIIYVLEVAKYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPY 371

Query: 370 VKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLI 429
             FLNRVR+ E KL+S GLW VPHPWLN+FVP SRI DF  GV   ++L +  T G  L 
Sbjct: 372 FDFLNRVRNEEDKLRSLGLWEVPHPWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLF 431

Query: 430 YPMNHSKWDSRNSTVIPNEEVFYTIGFLHSS-GFDDWKSFDRQNDEILEFCEKSGIEIKQ 489
           YP N +KW++R ST+ P+E+VFY IG L S+ G  +W+  +  ND++++FCE SGI+IK+
Sbjct: 432 YPTNRNKWNNRMSTMTPDEDVFYVIGLLQSAGGSQNWQELENLNDKVIQFCENSGIKIKE 491

Query: 490 YLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 520
           YL ++  +  W+ HFG KW +F   K+ FDPK +LSPGQ IFN
Sbjct: 492 YLMHYTRKEDWVKHFGPKWDDFLRKKIMFDPKRLLSPGQDIFN 524

BLAST of CmoCh12G013370 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 507.7 bits (1306), Expect = 1.1e-143
Identity = 248/485 (51.13%), Postives = 332/485 (68.45%), Query Frame = 0

Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 44  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 103

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 104 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 163

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT-GKGNMVTCSAKKNSELFHA 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVT GKG ++ CS ++N+ LFH 
Sbjct: 164 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEENTRLFHG 223

Query: 222 VLGGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYL 281
           VLGGLGQFGII RARI+LEPAP+RV+W+R+LY++F  F +DQE LIS + + +    DY+
Sbjct: 224 VLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYV 283

Query: 282 EGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEV 341
           EG V++     + WRSSFF P +  ++ S+++  S++YCLE+ K Y       +D+++E+
Sbjct: 284 EGFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEI 343

Query: 342 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDA 401
           L + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD 
Sbjct: 344 LMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDK 403

Query: 402 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKS--- 461
           GVFK I+   N T GP+LIYPMN  KWD R+S V P+EEVFY +  L S+  D  ++   
Sbjct: 404 GVFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKL 463

Query: 462 --FDRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSP 519
                QN  ILEFCE++ I +KQYLP+H TQ +W+ HFG KW  FR +K +FDP++IL+ 
Sbjct: 464 EYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILAT 517

BLAST of CmoCh12G013370 vs. TAIR 10
Match: AT2G19500.1 (cytokinin oxidase 2 )

HSP 1 Score: 492.3 bits (1266), Expect = 4.9e-139
Identity = 251/482 (52.07%), Postives = 319/482 (66.18%), Query Frame = 0

Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           DP  ++ AS D+GN+    P  V+ P+S  D+  L+ Y+ N    F VAARGQGHS+ GQ
Sbjct: 38  DPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQVAARGQGHSLNGQ 97

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVS 160
           A    GV+++M+ +        +++S+ +     YADV    LW+DVL      GV+PVS
Sbjct: 98  ASVSGGVIVNMTCI------TDVVVSKDKK----YADVAAGTLWVDVLKKTAEKGVSPVS 157

Query: 161 WTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVL 220
           WTDYL+ITVGGTLSNGGI GQ FR GP VSNV+E+DV+TGKG M+TCS + N ELF+ VL
Sbjct: 158 WTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLTCSRQLNPELFYGVL 217

Query: 221 GGLGQFGIIARARIALEPAPKRVKWVRMLYTNFSAFAKDQERLISFNERKQAN--ALDYL 280
           GGLGQFGII RARI L+ APKR KW RMLY++F+ F KDQERLIS      AN   +DYL
Sbjct: 218 GGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLIS-----MANDIGVDYL 277

Query: 281 EGLVLLHHDSPDTWRSSFFPPSDHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEV 340
           EG + L +   DT   SFFPPSD  +V  L  Q+ +IY LEVAKYYD      I K ++ 
Sbjct: 278 EGQIFLSNGVVDT---SFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPIISKVIDT 337

Query: 341 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDA 400
           L + L Y  GF    DV Y  FLNRV   E KL+S GLW +PHPWLNL+VPKSRILDF  
Sbjct: 338 LTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKSRILDFHN 397

Query: 401 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIP--NEEVFYTIGFLHSSGFDDWKSF 460
           GV KDI+L++    G  L+YP N +KWD+R S +IP  +E+V Y IG L S+   D    
Sbjct: 398 GVVKDILLKQKSASGLALLYPTNRNKWDNRMSAMIPEIDEDVIYIIGLLQSATPKDLPEV 457

Query: 461 DRQNDEILEFCEKSGIEIKQYLPNHKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQR 519
           +  N++I+ FC+ SGI+IKQYL ++ ++  WI+HFGSKW +F   K  FDPK +LSPGQ 
Sbjct: 458 ESVNEKIIRFCKDSGIKIKQYLMHYTSKEDWIEHFGSKWDDFSKRKDLFDPKKLLSPGQD 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTS31.9e-16756.05Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1[more]
Q9FUJ26.4e-14450.48Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2[more]
Q67YU06.4e-14451.24Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Q5ZAY92.5e-14051.23Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 ... [more]
Q9FUJ36.9e-13852.07Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FD080.0e+00100.00Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111444271 PE=3 SV=1[more]
A0A6J1HKZ42.3e-30196.15Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111465505 PE=3 SV=1[more]
A0A1S3C9Z61.6e-26283.37Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498521 PE=3 SV=1[more]
A0A6J1G4H47.9e-26283.33Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111450699 PE=3 SV=1[more]
A0A6J1DPL81.8e-25381.45Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021923 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56970.11.3e-16856.05cytokinin oxidase 3 [more]
AT1G75450.14.6e-14551.24cytokinin oxidase 5 [more]
AT4G29740.24.6e-14550.48cytokinin oxidase 4 [more]
AT1G75450.21.1e-14351.13cytokinin oxidase 5 [more]
AT2G19500.14.9e-13952.07cytokinin oxidase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 111..241
e-value: 1.2E-199
score: 665.8
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 242..477
e-value: 1.2E-199
score: 665.8
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 41..518
e-value: 1.2E-199
score: 665.8
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 60..208
e-value: 1.9E-21
score: 76.3
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 240..518
e-value: 7.6E-115
score: 383.1
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 9..519
NoneNo IPR availablePANTHERPTHR13878:SF115CYTOKININ DEHYDROGENASE 1, FAD AND CYTOKININ-BINDING PROTEINcoord: 9..519
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 60..95
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 56..239
score: 15.235473
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 35..238
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 240..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G013370.1CmoCh12G013370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity