CmoCh12G013260 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G013260
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAmino_oxidase domain-containing protein
LocationCmo_Chr12: 11561849 .. 11570538 (+)
RNA-Seq ExpressionCmoCh12G013260
SyntenyCmoCh12G013260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGAGAGGAAGGCGAAAATTGTGATTATTGGCGCCGGAATGGCGGGACTCACGGCGGCGAATAGGCTCTACACGGCGCCCGGTTCGGCGGATTTGTTCGATATCTCCGTCGTGGAGTTCATGGGCCAGAAAATAGAAATGGGTGCCACGTGGATCCACGGTATCCGCGGCAGCCCAATTCATGAAATCGCCGATCAAATCAACGCCCTTCATTCTGACCAGCCATGGGAGTTCATGAACGCCTGGTCCGCCCACTCTACTACCGTCGCAGAAGGTGGCGTCGAGATCTCGCCGGCCATCATCGACCCCGTTTCCTCTCTCTTCACGAACCTCATTGATTTCTCTCAGGGAAAAATCGCCGGCGACTCCGACTTTCTCCGGCAAGCAAAGTCCGACAAGCTCAGCATCGGAGCTTATCTCCGGCGAGGCCTTGATTCGTACTTGATTTCTAAAAATGGCGAAATCGAGTTCAACGAGTGTAAAGGATGGAACCAAAAATCCCTCGAGGAAGCTATTTTCGCCATGTATGAAAACATCCAAAGAACTTACACCTCCGCCGGAGAGCTCTCGACGGTGGATCTCGTTGCGGAAAGTGAGTACCAAGTGTTCCCCGGCGATGAAATCACCATCCCCAAAGGCTATTCGACCGTAATCCAATCAATCGCCTCTATTCTTCCCCCTGGTTTAGTCCAATTGAACAAAAATGTCACCAAAATCGAATGGCATCCCGAACCAGACGCCCGGAATGTTCCAACGCCGGTGAGAATCCATTTTTCCGACGGGTCCGACATATCCGCCGATCACGTAATCGTCACCGTTTCCCTCGTAGTCCTCAAGGCTGGAACGCAACAAGAATCCGGTATGTTTAATCCGCCGTTGCCGTCGTTCAAAACAGAGGCGATTTCCCGGCTCGGATGTTGACTCTGGCAAATCGGATGTCGACGAAAAAATTGGCCGAAGGTTTCTTCGAAGGAAAAATTAGCGGAAGTAAAAGGAGAGAGAGAGAGATTCGCTGGTAATGCTGAAGACTCGATATATTTATTTTACATCCGTTGAATGGTTTAAATAATAAATCTAATTACAAAAATAGAAAAACCAAATATACAAAAATAGAATAAGTGGACGAAGACTCCGCCACTCTGCCAAAACAATGGCCCCTAGAATCACGCTCCTCCATTCGCGTGAACTCATATGTTTTTACAAACCTAACTAAATAAGGCAGAACTTTTATTTAACAATCTCCTCCTAAAAGCTGTACCTTATTATTTGAACTCCGATCTTTGAGCGTAGTTCTTCAAATTTCACCCGCGCCAGGGACTTGGTGAAAATGTCTCCAAGTTGTTCCTCTGTTCGGATGAAATCAATTTTGATGAGCCCTCGATCTGCACATTCTCGAATGTAGTGGAATCTGGTTTCTATGTGCTTGCTCCGGTCGTGCAAAATTGGATTTTTGATCAGGGAGATCGTAGCTTTGTTGTCTACGTATAGCATTGGTGGATCGCTTTCTCTCCCGATGAGTTCTTCCATTAGTCGTGCAAGCCAGATCCCTTGAGTTGCTGCCATCGAAGCGGCGATGTATTCTGCTTCGCAGGAAGACAAAGCAACTACCTTCTGTTTTGTTGATTGCCAGCAGATCGCGCCGCCTGAGAGAAAGTAGATCATTCCGCTGGTGCTCTTACGATCATCAACGTCACCGGCCATGTCACTATCGCTGTAGTCGACCAGTTTGAGTTTCTCCTTTTCGCTCCCAGCGCAGTATCTTACGCCCCAGCCTCTGGTTCCGGCTACATAGCGCAGGATGCGCTTGACAGCCACTAGATGCTCCTCCCTAGGTGCTTCCATAAACCTGCTCACATATCCAACAGAATAAGCAAGATCAGGACGAAAGTTTACCAGATAGCGCAGACTCCCGACAATGCTGCGGTAATTGGTGGAGTCGACTGTCGTCGTAGTGCCGGCCTTCCTTAGTTGAAGTCGGGCCTCCATTGGCGTCCTGGTAGGATTACTGTCCACAAGCCCAGTTGTGTCCAGCAGCTTCCTCGCGTATGCACTTTGGCAGATGGAGATGCCAGAAGAGTTCTGTTGCACTTCGATGCCGAGGTAGTAGCTGAGCACACCGAGATCGCTCATCTCGAAGTTTTTCGACATCTCCTTCTTGAACCTTCCGAGGACTCCCACGTCGCCTCCAGTGATTATGAGGTCGTCGACGTACACTCCCACGATCAGTCGCTTTTTGCCGTGGCCGTACGTGTACATGCCATGCTCAGAGGCACAACGTTTGAAATTCAGTGACAGTAGGGTACTGTCGAGCTTCGCGTTCCAGGCTCGTGGGGCTTGTCGAAGCCCGTAGAGTGCTTTGTGCAGGCGCAGTACCTTATTAGGGTTGTCGTTATCCAGGAAGCCAGGTGGTTGTCGAACATAGACGGTCTCCTTCAACTCTCCGTTAAGGAAAGCAGACTTTACGTCCATATGGTGAACCTCCCAAGAGTGATGTGCTGCAATTGCCAGCAGGAAACGAACGGATTCTAACCTTGCTACTGGCGCAAATACCTCTTCGAAATCCACTCCTTGCTTCTGGACGTAGCCCTTCGCCACCAGACGAGCCTTGTGCTTCACAACTTCTCCTTTTTCGTTGCGCTTCAGTTTGAAGACCCATTTGAGCCCTATGGCTCGGTGTCCCGGTGGCATATCCTCCAGACTCCACGTCTGGTTCTCAGTGATGGATGTCATCTCCTCCTGCATTGCCTTCCGCCAGCACGGGTTCTTTTCTGCTTCGGCGAAGGTGTTTGGTTCATCTGCACTGACGGCATGTAGTTCGGCCACTTCCTCGAGCTCGCGCGCTGCTAGTCCAGGTGGTTCACCTCCTCCCACTAGGTCATCCATCCTCCGGTACCTAGCCTCCAGATCAGTATCGTGATCGGCATCCAGCGTCGAATCCGCAGTCCGTGGTGTTGCGAATTCCACTGGTTCAGGTGGTGCACCTGCTGGCGGCGGTGACGTCTCCTGATGCTGGGCTCCTCCTTCTTCAGGCTCGGTGACGAGGTACTCCACCGTGAATTGATTTGGATTACGGTCTGTCTCGATCACGTCATTCCACTGCCAGAAGGTGCTTTCGTCGAAGACGACGTCGCGAGACACGTGAGCTCGCCCCCCCACAGGATCATAGAGTCTGTACGCCTTGCTCCCGGGTTCGTAGCCGATGAAGACGACCTTCAGCCCCCTGGGATCGAGCTTGGCGAGATGGGGACGTGTTACCTTCATGTATGCGACGCAGCCGAACACGCGAAAATGATGTACTGTTGGTTTTTTGTTGTACCAGGCTTCATATGGCGTCTTCCCGTCGAGGCTTCGGGTTGGTGACCGATTGAGGAGATAATCTCCCCAGAATCTCCCAGGCATCCCGGCCGTCACCAACAATGACCTCGCTGTCCCGACAATGGTCTGATTTCGGCGCTCTACCACTCCGTTCTGTTGGGGGGAGTAGGGCGCCGTTAGGTGCCGTTGTATGCCGATCTCGTCGCAGTACTTATTGAAACTTGCCGAGGTGAATTCTCCGCCTCGGTCTGTGCGCAGCACTCGCATCTTCTTCTCACATTCGGCCTCCGCTCGCGCTTTAATGCGCTTAACCGCCTCTGCCGCCTCACTTTTCGCTTGCAGCAGAGTCAGCCACATGAAGCGGCTTTTGTCATCGACTAACAGGAGGAAGAGACTCTTACCGCCTGGGGTCGCCGGCTTGATGGGCCCGCAAATATCGCCGTGTACAAGCTCCAATGGCTCATAGGCTCGGTAGGCTGTTCGAGACGGAAAGGGTGTGCGCCTCTGTTTGCCGATGAGGCACCCGTCGCACAGCTTGTTCACGCCTTTGATTTCTGGCAAGCCGTGCACCAACTCCTTCTTCTGTAGCTTTTCTAGGGCAGGAAAGTTTAAGTGTCCGTACCTTGCGTGCCACCTCCAAGATACCTCTTCGGTCTTGGCCGAGAGGCTAACGGGTTGGTCTATCTCTAACTCCAGGATGTAAAGACGGTTTGTGGTGCGCCTTGCCTGCGTGAGCAGCCGTCGTTGATTATCGCAGATTTTCAGTAGTCCGCGCTCGATGGAAATGAAGCAGCCTGTCTCATCGAGCTGACCCAGGCTCACAAGGTTAGCCTTGAGCCTCGGGATGAAGTAGACGTCGGTCAGCTTGCGATGCTCGCCTCCCTTACTGACGAACAGGATGGTGCCGCGCCCTTCGATCTCGACGACGGAGCCGTCGCCGAATTTCACCGTCCCACGGATCCCCGAGTCGAGCTCGGAGAACGCAGATCTAGCCCCGGTCATATGGTTTGTTGCCCCTGTGTCAAGGATCCATTGCTGGTGCTCGTGTTGCTCGTCCCTTTCGCCGATCTGAGCGCCCACTCGCTCCTCCTCCAGTTGAATTGGCTCCCCAGTTGGTGCCAGCTTTGCTACGCCCAACTGGAGCTCTTTCTTGGGTTCAACATCGTCGTCGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGGTGGAGAGGAAGGCGAAAATTGTGATTATTGGCGCCGGAATGGCGGGACTCACGGCGGCGAATAGGCTCTACACGGCGCCCGGTTCGGCGGATTTGTTCGATATCTCCGTCGTGGAGTTCATGGGCCAGAAAATAGAAATGGGTGCCACGTGGATCCACGGTATCCGCGGCAGCCCAATTCATGAAATCGCCGATCAAATCAACGCCCTTCATTCTGACCAGCCATGGGAGTTCATGAACGCCTGGTCCGCCCACTCTACTACCGTCGCAGAAGGTGGCGTCGAGATCTCGCCGGCCATCATCGACCCCGTTTCCTCTCTCTTCACGAACCTCATTGATTTCTCTCAGGGAAAAATCGCCGGCGACTCCGACTTTCTCCGGCAAGCAAAGTCCGACAAGCTCAGCATCGGAGCTTATCTCCGGCGAGGCCTTGATTCGTACTTGATTTCTAAAAATGGCGAAATCGAGTTCAACGAGTGTAAAGGATGGAACCAAAAATCCCTCGAGGAAGCTATTTTCGCCATGTATGAAAACATCCAAAGAACTTACACCTCCGCCGGAGAGCTCTCGACGGTGGATCTCGTTGCGGAAAGTGAGTACCAAGTGTTCCCCGGCGATGAAATCACCATCCCCAAAGGCTATTCGACCGTAATCCAATCAATCGCCTCTATTCTTCCCCCTGGTTTAGTCCAATTGAACAAAAATGTCACCAAAATCGAATGGCATCCCGAACCAGACGCCCGGAATGTTCCAACGCCGGTGAGAATCCATTTTTCCGACGGGTCCGACATATCCGCCGATCACGTAATCGTCACCGTTTCCCTCGTAGTCCTCAAGGCTGGAACGCAACAAGAATCCGAATTGAAGAGTATCAACAAATCTCCTTGCCTGAATTTCGTTTTCCACCGGCCGGATTCGGAATTTCGACGGAAAAAAATCCCATGGTGGATAAGAAAAACCAGTTTCCTCCGACCAATCTACCAAAATTCGTCAATATTATTGTCCTGGTTCGCCGGAGAAGAAGCTCTTCACCTGGGAAAACTCAACGAGGAAGAAGTCATCAATGGAGTTTCAACAACCATCAGTAACTTCCAAATTCCGAATTCAGAATCAGATTGCAATGACTGCGAAGCAAATTCGAAGGGGAGCGTTAAATTCAGTAAAGTATTGAAGAGCCAATGGGGCAGCGATCCTCTGTTTCAGGGTTCATACTCATACATTGCAGTGGGATCAAGCGGGGAAGATTTGGACGCCATGGCAGAGCCATTACCAAGAACAGAGGAATCTCCAAAAGCTCCATTACTTCAGATTCTGTTTGCAGGTGAAGCAACTCACAGAACCCATTATTCTACAACACATGGAGCTTATTTCAGTGGCCTTAGAGAGGCCATTAGGCTGCTTCACCATTACAATTGCATTTGA

Coding sequence (CDS)

ATGGTGGAGAGGAAGGCGAAAATTGTGATTATTGGCGCCGGAATGGCGGGACTCACGGCGGCGAATAGGCTCTACACGGCGCCCGGTTCGGCGGATTTGTTCGATATCTCCGTCGTGGAGTTCATGGGCCAGAAAATAGAAATGGGTGCCACGTGGATCCACGGTATCCGCGGCAGCCCAATTCATGAAATCGCCGATCAAATCAACGCCCTTCATTCTGACCAGCCATGGGAGTTCATGAACGCCTGGTCCGCCCACTCTACTACCGTCGCAGAAGGTGGCGTCGAGATCTCGCCGGCCATCATCGACCCCGTTTCCTCTCTCTTCACGAACCTCATTGATTTCTCTCAGGGAAAAATCGCCGGCGACTCCGACTTTCTCCGGCAAGCAAAGTCCGACAAGCTCAGCATCGGAGCTTATCTCCGGCGAGGCCTTGATTCGTACTTGATTTCTAAAAATGGCGAAATCGAGTTCAACGAGTGTAAAGGATGGAACCAAAAATCCCTCGAGGAAGCTATTTTCGCCATGTATGAAAACATCCAAAGAACTTACACCTCCGCCGGAGAGCTCTCGACGGTGGATCTCGTTGCGGAAAGTGAGTACCAAGTGTTCCCCGGCGATGAAATCACCATCCCCAAAGGCTATTCGACCGTAATCCAATCAATCGCCTCTATTCTTCCCCCTGGTTTAGTCCAATTGAACAAAAATGTCACCAAAATCGAATGGCATCCCGAACCAGACGCCCGGAATGTTCCAACGCCGGTGAGAATCCATTTTTCCGACGGGTCCGACATATCCGCCGATCACGTAATCGTCACCGTTTCCCTCGTAGTCCTCAAGGCTGGAACGCAACAAGAATCCGAATTGAAGAGTATCAACAAATCTCCTTGCCTGAATTTCGTTTTCCACCGGCCGGATTCGGAATTTCGACGGAAAAAAATCCCATGGTGGATAAGAAAAACCAGTTTCCTCCGACCAATCTACCAAAATTCGTCAATATTATTGTCCTGGTTCGCCGGAGAAGAAGCTCTTCACCTGGGAAAACTCAACGAGGAAGAAGTCATCAATGGAGTTTCAACAACCATCAGTAACTTCCAAATTCCGAATTCAGAATCAGATTGCAATGACTGCGAAGCAAATTCGAAGGGGAGCGTTAAATTCAGTAAAGTATTGAAGAGCCAATGGGGCAGCGATCCTCTGTTTCAGGGTTCATACTCATACATTGCAGTGGGATCAAGCGGGGAAGATTTGGACGCCATGGCAGAGCCATTACCAAGAACAGAGGAATCTCCAAAAGCTCCATTACTTCAGATTCTGTTTGCAGGTGAAGCAACTCACAGAACCCATTATTCTACAACACATGGAGCTTATTTCAGTGGCCTTAGAGAGGCCATTAGGCTGCTTCACCATTACAATTGCATTTGA

Protein sequence

MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVVEFMGQKIEMGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQKSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILPPGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQESELKSINKSPCLNFVFHRPDSEFRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIPNSESDCNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREAIRLLHHYNCI
Homology
BLAST of CmoCh12G013260 vs. ExPASy Swiss-Prot
Match: Q9SU79 (Probable polyamine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PAO5 PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 4.5e-136
Identity = 274/539 (50.83%), Postives = 346/539 (64.19%), Query Frame = 0

Query: 4   RKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIEMGA 63
           +KA+IVIIGAGMAGLTAAN+LYT+  S + F++SVV             EF  +KIEMGA
Sbjct: 3   KKARIVIIGAGMAGLTAANKLYTS--SNNTFELSVVEGGSRIGGRINTSEFSSEKIEMGA 62

Query: 64  TWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLFT 123
           TWIHGI GSP++ IA +  +L SD+PWE M++    + T AEGG EI P+I++ +S LFT
Sbjct: 63  TWIHGIGGSPVYRIAKETGSLVSDEPWECMDSTIDKAKTFAEGGFEIEPSIVESISGLFT 122

Query: 124 NLIDFSQGK--IAGDSDFLRQA-----------KSDKLSIGAYLRRGLDSY--LISKNGE 183
            L++ +QGK     D+D  R A           K    S+G++L+ G D+Y   IS  GE
Sbjct: 123 ALMELAQGKEISQSDADLSRLAHIYETATRVCSKGSSTSVGSFLKSGFDAYWDSISNGGE 182

Query: 184 IEFNECKGWNQKSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGY 243
                   W++KSLEEAIF M+ N QRTYTSA ELST+D  AESEYQ+FPG+EITI KGY
Sbjct: 183 EGVKGYGKWSRKSLEEAIFTMFSNTQRTYTSADELSTLDFAAESEYQMFPGEEITIAKGY 242

Query: 244 STVIQSIASILPPGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVS 303
            +VI  +AS+LP G++QLN+ VTKIEW            V++HFSDGS + ADHVIVTVS
Sbjct: 243 LSVIHHLASVLPQGVIQLNRKVTKIEWQ--------SNEVKLHFSDGSVVFADHVIVTVS 302

Query: 304 LVVLKAGTQQESEL---------------------------KSINKSPCLNFVFHRPDSE 363
           L VLKAG + ++EL                            S  K P L  VF R DSE
Sbjct: 303 LGVLKAGIETDAELFSPPLPDFKSDAIRRLGYGVVNKLFVEMSQRKFPSLQLVFDREDSE 362

Query: 364 FRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIP 423
           FR  KIPWW+R+T+ + PI+ NS +LLSWFAG+EAL L KL +EE+ + V TTIS     
Sbjct: 363 FRFVKIPWWMRRTATITPIHSNSKVLLSWFAGKEALELEKLTDEEIKDAVMTTISCLTGK 422

Query: 424 NSESD-----CNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMAEP 474
             ++D      N    +   ++K +KVLKS+WGSDPLF+GSYSY+AVGSSG+DLDAMAEP
Sbjct: 423 EVKNDTAKPLTNGSLNDDDEAMKITKVLKSKWGSDPLFRGSYSYVAVGSSGDDLDAMAEP 482

BLAST of CmoCh12G013260 vs. ExPASy Swiss-Prot
Match: Q5NAI7 (Polyamine oxidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAO1 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.5e-86
Identity = 201/541 (37.15%), Postives = 294/541 (54.34%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MV +K ++V++GAG++GL AA+RL  A G  D F+++VV             EF G ++E
Sbjct: 1   MVAKKPRVVVVGAGISGLAAAHRLCGAGG--DRFEVAVVEAGDRVGGRILTSEFAGHRVE 60

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQ----PWEFMNAWSAHSTTVAEGG-VEISPAII 120
           MGATW+ G+ GSP++ +A    AL  ++    P+E M+ +     TVAEGG V  +  + 
Sbjct: 61  MGATWVQGVVGSPVYALARDAGALGEEEGRGLPYERMDGFPDRVLTVAEGGEVVDADTVA 120

Query: 121 DPVSSLFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECK 180
            P+  L+  +++ ++   AG              +  YLRRGL +Y  +++         
Sbjct: 121 GPIEELYRGMMEAARAGEAGGGG----------GVEEYLRRGLRAYQAARSA--GGGGGG 180

Query: 181 GWNQKSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSI 240
           G   + ++EA+ AM+ N +RT TSA +L  +DL AE EY+ FPG+ +TIP GYS V++ +
Sbjct: 181 GKELEEVDEALLAMHINRERTDTSADDLGDLDLTAEGEYRDFPGEHVTIPGGYSRVVERL 240

Query: 241 ASILPPGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGS-DISADHVIVTVSLVVLKA 300
           A+ LPPG V+L   + +++W          TPVR+HF+DG+  ++ADHVI+TVSL VLKA
Sbjct: 241 AAALPPGTVRLGLRLRRLKWG--------GTPVRLHFADGAPPLTADHVILTVSLGVLKA 300

Query: 301 GTQQES------------------------------------ELKSINKS---------- 360
               +                                     E++++  S          
Sbjct: 301 SLGNKDTAGVGAAAIAFDPPLPPFKREAVARLGFGVVNKLFMEVEAVAPSEPEDVAGVQP 360

Query: 361 PCLNFVFHRPDSEFRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVI 420
               F F          KIPWW+R T  + P++  S++ L+WFAG EA HL  L +++VI
Sbjct: 361 AAAGFPFLHMAFRGHVSKIPWWMRGTESICPVHAGSTVALAWFAGREAAHLESLPDDDVI 420

Query: 421 NGVSTTISNFQIPNSESDCNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGE 472
            G   T+ +F             A  +  V+  ++ +S W +DPLF GSYSY+AVGSSG+
Sbjct: 421 RGAHATLDSF-----------LPAAPRWRVR--RIKRSGWATDPLFLGSYSYVAVGSSGD 480

BLAST of CmoCh12G013260 vs. ExPASy Swiss-Prot
Match: Q99K82 (Spermine oxidase OS=Mus musculus OX=10090 GN=Smox PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 6.0e-48
Identity = 161/558 (28.85%), Postives = 246/558 (44.09%), Query Frame = 0

Query: 7   KIVIIGAGMAGLTAANRLYTAPGSADLFDISVVEFMGQKI----------EMGATWIHGI 66
           ++V+IGAG+AGL AA R     G  D+  +     +G ++          E+GATWIHG 
Sbjct: 26  RVVVIGAGLAGLAAA-RALLEQGFTDVTVLEASSHIGGRVQSVRLGDTTFELGATWIHGS 85

Query: 67  RGSPIHEIADQINALHSDQPWE----FMNAWSAHSTT--VAEGGVEISPAIIDPVSSLFT 126
            G+PI+++A+    L      E     ++ +S +     +   G  I   +++  S L+ 
Sbjct: 86  HGNPIYQLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNRGCRIPKDVVEEFSDLYN 145

Query: 127 NLIDFSQGKIAGDSDFLRQAK----SDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQ 186
            + + +Q       +F R  K      + S+G + R  + + +     + E         
Sbjct: 146 EVYNMTQ-------EFFRHGKPVNAESQNSVGVFTREKVRNRIRDDPDDTEAT------- 205

Query: 187 KSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASIL 246
           K L+ A+   Y  ++   +S+  +  V L A  E+   PG    IP G+  V++ +A  +
Sbjct: 206 KRLKLAMIQQYLKVESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGI 265

Query: 247 PPGLVQLNKNVTKIEW-----HP-----EPDA------------------------RNVP 306
           PP ++QL K V  I W     HP     EP                           + P
Sbjct: 266 PPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEP 325

Query: 307 TPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQESELKSINKSPCLN----FVFHR---- 366
            PV +   D   I ADHVIVTVSL VLK   Q  S  +     PCL        HR    
Sbjct: 326 WPVVVECEDCEVIPADHVIVTVSLGVLK--RQYTSFFR-----PCLPTEKVAAIHRLGIG 385

Query: 367 -PDSEFRRKKIPWWIRKTSFLRPIYQNSS------------------------------I 426
             D  F   + P+W  + + L+ ++++ +                              +
Sbjct: 386 TTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERYGHV 445

Query: 427 LLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIPNSESDCNDCEANSKGSVKFSKVLKS 472
           L  W  GEEAL + + ++E V    +  +  F               +    K  ++L+S
Sbjct: 446 LSGWICGEEALVMERCDDEAVAEICTEMLRQF-------------TGNPNIPKPRRILRS 505

BLAST of CmoCh12G013260 vs. ExPASy Swiss-Prot
Match: Q865R1 (Peroxisomal N(1)-acetyl-spermine/spermidine oxidase OS=Bos taurus OX=9913 GN=PAOX PE=1 SV=3)

HSP 1 Score: 193.0 bits (489), Expect = 7.9e-48
Identity = 158/527 (29.98%), Postives = 242/527 (45.92%), Query Frame = 0

Query: 4   RKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVVEFMGQKI----------EMGATWI 63
           R  +++++G G+AGL AA RL   P  + L  +      G +I          E+GA WI
Sbjct: 13  RGPRVLVVGGGIAGLGAAQRLCRHPAFSHLRVLEATARAGGRIRSEHSFGGVVEVGAHWI 72

Query: 64  HG-IRGSPIHEIADQINAL------HSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVS 123
           HG  +G+P+ ++A +   L        +Q  E        S + A  GV +S  ++  ++
Sbjct: 73  HGPSQGNPVFQLAAKYGLLGEKALSEENQLIETGGHVGLPSVSYASSGVSVSLELVAEMA 132

Query: 124 SLFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQ 183
           SLF +LID ++       +FL+ A++   S+G YL+  +  ++             GW +
Sbjct: 133 SLFYSLIDQTR-------EFLQAAETTPPSVGEYLKEKIRQHM------------AGWTE 192

Query: 184 ----KSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSI 243
               K L+ AI     N++   +    +  V L    EY V PG + T P+GY  +   I
Sbjct: 193 DEETKKLKLAILKNLFNVECCVSGTHSMDLVALAPFGEYTVLPGLDCTFPEGYQGLTDCI 252

Query: 244 ASILPPGLVQLNKNVTKIEWH---PEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVL 303
            + LP  ++  +K V  I W+    E  A     PV +   DG    A HV+VTV L   
Sbjct: 253 MASLPKDVMVFDKPVKTIHWNGSFREASAPGETFPVLVECEDGDCFPAHHVVVTVPLGFF 312

Query: 304 K----------AGTQQESELKSI----NKSPCLNF--VFHRPDSEFRRKKIPW------- 363
           K            T++   ++ I    N    L F   F  PD +    ++ W       
Sbjct: 313 KKHLDTFFEPPLPTEKVEAIRKIGFGTNNKIFLEFEEPFWEPDCQ--HIQVVWEDMSPLE 372

Query: 364 ---------WIRKT--SFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTI--- 423
                    W +K    ++ P +Q S +L  + AG E+  +  L++E+V+  ++  +   
Sbjct: 373 DTAPELQDAWFKKLIGFWVLPPFQASHVLCGFIAGLESEFMETLSDEDVLRSLTQVLRRV 432

Query: 424 -SNFQIPNSESDCNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMA 469
             N Q+P   S                 +L+S W S P  +GSYSY+AVGSSG+D+D +A
Sbjct: 433 TGNPQLPAPRS-----------------MLRSCWHSAPYTRGSYSYVAVGSSGDDMDRLA 492

BLAST of CmoCh12G013260 vs. ExPASy Swiss-Prot
Match: Q9NWM0 (Spermine oxidase OS=Homo sapiens OX=9606 GN=SMOX PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 8.1e-45
Identity = 155/547 (28.34%), Postives = 239/547 (43.69%), Query Frame = 0

Query: 7   KIVIIGAGMAGLTAANRLYTAPGSADLFDISVVEFMGQKI----------EMGATWIHGI 66
           ++V+IGAG+AGL AA  L    G  D+  +     +G ++          E+GATWIHG 
Sbjct: 26  RVVVIGAGLAGLAAAKAL-LEQGFTDVTVLEASSHIGGRVQSVKLGHATFELGATWIHGS 85

Query: 67  RGSPIHEIADQINALHSDQPWE----FMNAWSAHSTT--VAEGGVEISPAIIDPVSSLFT 126
            G+PI+ +A+    L      E     ++ +S +     +   G  I   +++  S L+ 
Sbjct: 86  HGNPIYHLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNHGRRIPKDVVEEFSDLYN 145

Query: 127 NLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQKSLE 186
            + + +Q     D     ++++   S+G + R  + + + +   + E         K L+
Sbjct: 146 EVYNLTQEFFRHDKPVNAESQN---SVGVFTREEVRNRIRNDPDDPEAT-------KRLK 205

Query: 187 EAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILPPGL 246
            A+   Y  ++   +S+  +  V L A  E+   PG    IP G+  V++ +A  +P  +
Sbjct: 206 LAMIQQYLKVESCESSSHSMDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPAHV 265

Query: 247 VQLNKNVTKIEWH--------PEPDAR--------------------------NVPTPVR 306
           +QL K V  I W         PE + R                          +    V 
Sbjct: 266 IQLGKPVRCIHWDQASARPRGPEIEPRGEGDHNHDTGEGGQGGEEPRGGRWDEDEQWSVV 325

Query: 307 IHFSDGSDISADHVIVTVSLVVLK----------AGTQQESELK--SINKSPCLNFVFHR 366
           +   D   I ADHVIVTVSL VLK            T++ + +    I  +  +   F  
Sbjct: 326 VECEDCELIPADHVIVTVSLGVLKRQYTSFFRPGLPTEKVAAIHRLGIGTTDKIFLEFEE 385

Query: 367 P--DSEFRRKKIPW---------------WIRKT---SFLRPIYQNSSILLSWFAGEEAL 426
           P    E    +  W               W RK      L P  +   +L  W  GEEAL
Sbjct: 386 PFWGPECNSLQFVWEDEAESHTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEAL 445

Query: 427 HLGKLNEEEVINGVSTTISNFQIPNSESDCNDCEANSKGSVKFSKVLKSQWGSDPLFQGS 472
            + K ++E V    +  +  F               +    K  ++L+S WGS+P F+GS
Sbjct: 446 VMEKCDDEAVAEICTEMLRQF-------------TGNPNIPKPRRILRSAWGSNPYFRGS 505

BLAST of CmoCh12G013260 vs. ExPASy TrEMBL
Match: A0A6J1G2I5 (probable polyamine oxidase 5 OS=Cucurbita moschata OX=3662 GN=LOC111450217 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.6e-205
Identity = 375/535 (70.09%), Postives = 428/535 (80.00%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MVE+KAKIVIIGAGMAGL AAN+LYTAPGS  LFDISVV             EFMG++IE
Sbjct: 1   MVEKKAKIVIIGAGMAGLMAANKLYTAPGSQHLFDISVVEGGGRIGGRINTAEFMGERIE 60

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSS 120
           MGATWIHGI GSPI+EIAD+I AL+SDQPWE M+ +S  STTVAEGGVE+SPA +DP+S+
Sbjct: 61  MGATWIHGIGGSPIYEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPATVDPIST 120

Query: 121 LFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQK 180
           LF NL+DF+QGKIAG+S+FLR+   DKLS+GA+LRRGL+SY +SKNG  E N C  W+QK
Sbjct: 121 LFKNLMDFAQGKIAGESNFLRRGNYDKLSVGAFLRRGLESYWLSKNGVTEVNGCGEWSQK 180

Query: 181 SLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILP 240
           +LEEAIFAMYEN QRTYTSAGELST+D VAESEYQ+FPG+EITI KGY +VI+SIAS+LP
Sbjct: 181 ALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIESIASVLP 240

Query: 241 PGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQES 300
           PGLVQL K VTKIEW+PE ++RN+PTPV +HF+DGS  SADHVIVTVSL VLKAGT+++S
Sbjct: 241 PGLVQLGKKVTKIEWNPESESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKAGTREDS 300

Query: 301 EL--------------------------------------KSINKSPCLNFVFHRPDSEF 360
            L                                      KSINK PCLNFVFH+PDSEF
Sbjct: 301 ALFNPPLPSFKTEAISRLGFGVVNKLFLRLSPPTNDGDNPKSINKFPCLNFVFHQPDSEF 360

Query: 361 RRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIP- 420
           RRK+IPWW+RKT+ L+PIYQNSSILLSWFAGEEAL L KL ++E+INGVSTTISNF IP 
Sbjct: 361 RRKRIPWWMRKTTTLQPIYQNSSILLSWFAGEEALQLEKLKDDEIINGVSTTISNFLIPR 420

Query: 421 ----NSESDCN---DCEANSKGSVK--FSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDA 475
               NSESD N   + + N   +V+  FS+VLKSQWGSDPLFQGSYSY+AVGSSGEDLDA
Sbjct: 421 SQNENSESDSNGWSNGQRNQNDTVEFHFSQVLKSQWGSDPLFQGSYSYVAVGSSGEDLDA 480

BLAST of CmoCh12G013260 vs. ExPASy TrEMBL
Match: A0A6J1KFF8 (probable polyamine oxidase 5 OS=Cucurbita maxima OX=3661 GN=LOC111493815 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 373/535 (69.72%), Postives = 425/535 (79.44%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MVE+KAKIVIIGAGMAGL AAN+LYTAPGS DLFDI+VV             EFMG++IE
Sbjct: 32  MVEKKAKIVIIGAGMAGLMAANKLYTAPGSKDLFDIAVVEGGGRIGGRINTAEFMGERIE 91

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSS 120
           MGATWIHGI GSPIHEIAD+I AL+SDQPWE M+ +S  STTVAEGGVE+SPA +DP+S+
Sbjct: 92  MGATWIHGIGGSPIHEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPATVDPIST 151

Query: 121 LFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQK 180
           LF NL+DF+QGKIAG+S+ L Q   DKLS+GA+LR+GL+SY +S+NG  E N C  W+QK
Sbjct: 152 LFKNLMDFAQGKIAGESNILHQGNYDKLSVGAFLRQGLESYWLSRNGVTEVNGCGEWSQK 211

Query: 181 SLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILP 240
           +LEEAIFAMYEN QRTYTSAGELST+D VAESEYQ+FPG+EITI KGY +VI+SIAS+LP
Sbjct: 212 ALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIESIASVLP 271

Query: 241 PGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQES 300
           PGLVQL K VTKIEW+PEP++RN+PTPV +HF+DGS  SADHVIVTVSL VLKAGT+++S
Sbjct: 272 PGLVQLGKKVTKIEWNPEPESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKAGTREDS 331

Query: 301 EL--------------------------------------KSINKSPCLNFVFHRPDSEF 360
            L                                      KS NK PCLNFVFH+PDSEF
Sbjct: 332 ALFNPPLPSFKTEAISRLGFGVVNKLFLRLSQPANDGDNPKSNNKFPCLNFVFHQPDSEF 391

Query: 361 RRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIP- 420
           RRKKIPWW+RK + L+PIYQNSSILLSWFAGEEAL L KL +EE+INGVSTTISNF IP 
Sbjct: 392 RRKKIPWWMRKITTLQPIYQNSSILLSWFAGEEALQLEKLKDEEIINGVSTTISNFLIPR 451

Query: 421 ----NSESDCN---DCEANSKGSVKF--SKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDA 475
               NSESD N   +   N   +V+F  ++VLKSQWGSDPLFQGSYSY+AVGSSGEDLDA
Sbjct: 452 SQNENSESDSNGWSNGHRNRNDTVEFQLNQVLKSQWGSDPLFQGSYSYVAVGSSGEDLDA 511

BLAST of CmoCh12G013260 vs. ExPASy TrEMBL
Match: A0A5A7UZX3 (Putative polyamine oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00930 PE=4 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.2e-200
Identity = 365/533 (68.48%), Postives = 412/533 (77.30%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MV +KAKIVIIGAGMAGLTAAN+LYTA GS DLFDISVV             EFMG++IE
Sbjct: 1   MVVKKAKIVIIGAGMAGLTAANKLYTAEGSKDLFDISVVEGGGRIGGRINTAEFMGERIE 60

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSS 120
           MGATWIHGI GSPI++IADQI AL SDQPWE M+ +S  STTVAEGGVE+SPA + P+S+
Sbjct: 61  MGATWIHGIGGSPIYKIADQIGALQSDQPWECMDGYSGQSTTVAEGGVELSPATVKPIST 120

Query: 121 LFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQK 180
           LF  L+DF+QGKI GDS+ L+QA  DK SIG +LR+GLDSY +SKNGE E N CK W+QK
Sbjct: 121 LFQKLMDFAQGKITGDSNILQQANYDKRSIGDFLRQGLDSYWVSKNGETELNGCKEWSQK 180

Query: 181 SLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILP 240
           SLEEA+FAMYEN QRTYTSAG+LST+D ++ESEYQ+FPG+EITI KGY +VI+SIAS+LP
Sbjct: 181 SLEEAVFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIESIASVLP 240

Query: 241 PGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQES 300
           PGLVQL K VTKIEWHPE D RN+PTPV +HF+DGS ISADHVIVTVSL VLKAGTQ  S
Sbjct: 241 PGLVQLGKKVTKIEWHPELDPRNIPTPVTLHFADGSHISADHVIVTVSLGVLKAGTQPVS 300

Query: 301 --------------------------------------ELKSINKSPCLNFVFHRPDSEF 360
                                                 +LK  ++ PCL FVFH+PDS+F
Sbjct: 301 PLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTDNGHDLKRTHQFPCLKFVFHQPDSKF 360

Query: 361 RRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIPN 420
           RRKKIPWW+RKT+ LRPIYQNSSILLSW AGEEALHL KL +EE+INGVSTTISNF I  
Sbjct: 361 RRKKIPWWMRKTTTLRPIYQNSSILLSWLAGEEALHLEKLKDEEIINGVSTTISNFLIQK 420

Query: 421 SESD--------CNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMA 475
           SES+        CN    +      FS+VLKSQWGSDPLF GSYSY+AVGSSGEDLDAMA
Sbjct: 421 SESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMA 480

BLAST of CmoCh12G013260 vs. ExPASy TrEMBL
Match: A0A1S3CB61 (probable polyamine oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103498503 PE=4 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.2e-200
Identity = 365/533 (68.48%), Postives = 412/533 (77.30%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MV +KAKIVIIGAGMAGLTAAN+LYTA GS DLFDISVV             EFMG++IE
Sbjct: 1   MVVKKAKIVIIGAGMAGLTAANKLYTAEGSKDLFDISVVEGGGRIGGRINTAEFMGERIE 60

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSS 120
           MGATWIHGI GSPI++IADQI AL SDQPWE M+ +S  STTVAEGGVE+SPA + P+S+
Sbjct: 61  MGATWIHGIGGSPIYKIADQIGALQSDQPWECMDGYSGQSTTVAEGGVELSPATVKPIST 120

Query: 121 LFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQK 180
           LF  L+DF+QGKI GDS+ L+QA  DK SIG +LR+GLDSY +SKNGE E N CK W+QK
Sbjct: 121 LFQKLMDFAQGKITGDSNILQQANYDKRSIGDFLRQGLDSYWVSKNGETELNGCKEWSQK 180

Query: 181 SLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILP 240
           SLEEA+FAMYEN QRTYTSAG+LST+D ++ESEYQ+FPG+EITI KGY +VI+SIAS+LP
Sbjct: 181 SLEEAVFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIESIASVLP 240

Query: 241 PGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQES 300
           PGLVQL K VTKIEWHPE D RN+PTPV +HF+DGS ISADHVIVTVSL VLKAGTQ  S
Sbjct: 241 PGLVQLGKKVTKIEWHPELDPRNIPTPVTLHFADGSHISADHVIVTVSLGVLKAGTQPVS 300

Query: 301 --------------------------------------ELKSINKSPCLNFVFHRPDSEF 360
                                                 +LK  ++ PCL FVFH+PDS+F
Sbjct: 301 PLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTDNGHDLKRTHQFPCLKFVFHQPDSKF 360

Query: 361 RRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIPN 420
           RRKKIPWW+RKT+ LRPIYQNSSILLSW AGEEALHL KL +EE+INGVSTTISNF I  
Sbjct: 361 RRKKIPWWMRKTTTLRPIYQNSSILLSWLAGEEALHLEKLKDEEIINGVSTTISNFLIQK 420

Query: 421 SESD--------CNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMA 475
           SES+        CN    +      FS+VLKSQWGSDPLF GSYSY+AVGSSGEDLDAMA
Sbjct: 421 SESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMA 480

BLAST of CmoCh12G013260 vs. ExPASy TrEMBL
Match: A0A0A0LFR4 (Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000460 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 1.0e-199
Identity = 361/534 (67.60%), Postives = 412/534 (77.15%), Query Frame = 0

Query: 1   MVERKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIE 60
           MV +KAKIVIIGAGMAGLTAAN+LYTA GS DLFDISVV             EFMG++IE
Sbjct: 1   MVVKKAKIVIIGAGMAGLTAANKLYTAEGSKDLFDISVVEGGGRIGGRINTAEFMGERIE 60

Query: 61  MGATWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSS 120
           MGATWIHGI GSPI++IA+QI ALHSDQ WE M+ +S  STTVAEGG+E+SPA +DP+S+
Sbjct: 61  MGATWIHGIGGSPIYKIAEQIGALHSDQSWECMDGYSGQSTTVAEGGIELSPATVDPIST 120

Query: 121 LFTNLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQK 180
           LF  L+DF+QGKI GDSD L+QA  DK SIG +L++G+DSY +SKNGE E N CK W+QK
Sbjct: 121 LFQKLMDFAQGKITGDSDILQQANYDKRSIGEFLQQGIDSYWVSKNGETEVNGCKEWSQK 180

Query: 181 SLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILP 240
           SLEEAIFAMYEN QRTYTSAG+LST+D ++ESEYQ+FPG+EITI KGY +VI+SIAS+LP
Sbjct: 181 SLEEAIFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIESIASVLP 240

Query: 241 PGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVSLVVLKAGTQQES 300
           PGLVQL K VTKIEWHPE D  N+PTPV +HF+DGS ISADHVIVTVSL VLKAGTQ +S
Sbjct: 241 PGLVQLGKKVTKIEWHPELDPPNIPTPVTLHFADGSHISADHVIVTVSLGVLKAGTQPDS 300

Query: 301 ----------------------------------------ELKSINKSPCLNFVFHRPDS 360
                                                    LK  ++ PCLNFVFH+PDS
Sbjct: 301 PSPLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTENGLNLKRTHQFPCLNFVFHQPDS 360

Query: 361 EFRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQI 420
           +FRRKKIPWW+RKT+ LRPIYQNSS+LLSW AGEEALHL KL ++E+INGVSTTISNF I
Sbjct: 361 KFRRKKIPWWMRKTTSLRPIYQNSSLLLSWLAGEEALHLEKLKDDEIINGVSTTISNFLI 420

Query: 421 PNSESD--------CNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDA 474
             SES+        CN    +      FS+VLKSQWGSDPLF GSYSY+AVGSSGEDLDA
Sbjct: 421 QKSESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGEDLDA 480

BLAST of CmoCh12G013260 vs. TAIR 10
Match: AT4G29720.1 (polyamine oxidase 5 )

HSP 1 Score: 486.1 bits (1250), Expect = 3.2e-137
Identity = 274/539 (50.83%), Postives = 346/539 (64.19%), Query Frame = 0

Query: 4   RKAKIVIIGAGMAGLTAANRLYTAPGSADLFDISVV-------------EFMGQKIEMGA 63
           +KA+IVIIGAGMAGLTAAN+LYT+  S + F++SVV             EF  +KIEMGA
Sbjct: 3   KKARIVIIGAGMAGLTAANKLYTS--SNNTFELSVVEGGSRIGGRINTSEFSSEKIEMGA 62

Query: 64  TWIHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLFT 123
           TWIHGI GSP++ IA +  +L SD+PWE M++    + T AEGG EI P+I++ +S LFT
Sbjct: 63  TWIHGIGGSPVYRIAKETGSLVSDEPWECMDSTIDKAKTFAEGGFEIEPSIVESISGLFT 122

Query: 124 NLIDFSQGK--IAGDSDFLRQA-----------KSDKLSIGAYLRRGLDSY--LISKNGE 183
            L++ +QGK     D+D  R A           K    S+G++L+ G D+Y   IS  GE
Sbjct: 123 ALMELAQGKEISQSDADLSRLAHIYETATRVCSKGSSTSVGSFLKSGFDAYWDSISNGGE 182

Query: 184 IEFNECKGWNQKSLEEAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGY 243
                   W++KSLEEAIF M+ N QRTYTSA ELST+D  AESEYQ+FPG+EITI KGY
Sbjct: 183 EGVKGYGKWSRKSLEEAIFTMFSNTQRTYTSADELSTLDFAAESEYQMFPGEEITIAKGY 242

Query: 244 STVIQSIASILPPGLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDISADHVIVTVS 303
            +VI  +AS+LP G++QLN+ VTKIEW            V++HFSDGS + ADHVIVTVS
Sbjct: 243 LSVIHHLASVLPQGVIQLNRKVTKIEWQ--------SNEVKLHFSDGSVVFADHVIVTVS 302

Query: 304 LVVLKAGTQQESEL---------------------------KSINKSPCLNFVFHRPDSE 363
           L VLKAG + ++EL                            S  K P L  VF R DSE
Sbjct: 303 LGVLKAGIETDAELFSPPLPDFKSDAIRRLGYGVVNKLFVEMSQRKFPSLQLVFDREDSE 362

Query: 364 FRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIP 423
           FR  KIPWW+R+T+ + PI+ NS +LLSWFAG+EAL L KL +EE+ + V TTIS     
Sbjct: 363 FRFVKIPWWMRRTATITPIHSNSKVLLSWFAGKEALELEKLTDEEIKDAVMTTISCLTGK 422

Query: 424 NSESD-----CNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMAEP 474
             ++D      N    +   ++K +KVLKS+WGSDPLF+GSYSY+AVGSSG+DLDAMAEP
Sbjct: 423 EVKNDTAKPLTNGSLNDDDEAMKITKVLKSKWGSDPLFRGSYSYVAVGSSGDDLDAMAEP 482

BLAST of CmoCh12G013260 vs. TAIR 10
Match: AT4G16310.1 (LSD1-like 3 )

HSP 1 Score: 96.7 bits (239), Expect = 5.4e-20
Identity = 130/532 (24.44%), Postives = 210/532 (39.47%), Query Frame = 0

Query: 7    KIVIIGAGMAGLTAANRLYTAPGSADLFDISVVE--------------FMGQKIEMGATW 66
            K+++IGAG AGLTAA  L         F ++V+E               +   +++GA+ 
Sbjct: 619  KVIVIGAGPAGLTAARHL-----QRQGFSVTVLEARSRVGGRVFTDRSSLSVPVDLGASI 678

Query: 67   IHGIRGSPIHEIADQINALHSDQ---PWEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLF 126
            I GI      E     + L  +Q       ++ +     TV   G ++   + D + + F
Sbjct: 679  ITGIEADVPSERMPDPSVLVCNQLGLELSVLHGFCPLYDTVT--GKKVPAELDDALQAEF 738

Query: 127  TNLIDFSQGKIAGDSDFL----RQAKSDKLSIGAYLRRGL-------DSYLISKNGEIE- 186
             +LID        D D L     + +++K+S+   L  GL       D   I K G +  
Sbjct: 739  NSLID--------DVDLLVEEIGKERANKMSLEDGLEYGLQRLRMPHDKVNIDKFGLLNS 798

Query: 187  ---------FNECKGWNQKSLE--EAIFAMYENIQRTYTSAGELSTVDLV---AESEYQV 246
                     F + + W    L   E     +      Y  A  L  V L     +  Y  
Sbjct: 799  SSKTGIRGPFMQDESWKDDFLNPLERRVMNWHFAHTEYGCAAVLKEVSLPHWNQDEFYGG 858

Query: 247  FPGDEITIPKGYSTVIQSIASILPPGLVQLNKNVTKIEWHPEPDAR-NVPTPVRIHFSDG 306
            F G    I  GYS V++S+A  L    + LNK V+ + +  +  A  N    VR+  S+G
Sbjct: 859  FGGPHAMIKGGYSRVVESLAEGLD---IHLNKIVSDVSYVSDVSAMDNSKHKVRVSTSNG 918

Query: 307  SDISADHVIVTVSLVVLKAGTQQES---------ELKSINKSPCLNFVFHRP-------- 366
             +   D V+VTV L  LKA T + S          +K +        V   P        
Sbjct: 919  CEYLGDAVLVTVPLGCLKAETIKFSPPLPDWKYASIKQLGFGVLNKVVLEFPTVFWDDSV 978

Query: 367  --------DSEFRRKKIPWW-IRKTSFLRPIYQNSSILLSWFAGEEALHLGKLNEEEVIN 426
                    +++ R +   +W ++KT         + +L++   G+ A      ++ E +N
Sbjct: 979  DYFGATAEETDLRGECFMFWNVKKT-------VGAPVLIALVVGKAAFEYTNKSKSEHVN 1038

Query: 427  GVSTTISNFQIPNSESDCNDCEANSKGSVKFSKVLKSQWGSDPLFQGSYSYIAVGSSGED 469
                 +             D   +   SV       + WG+DP   G+YSY+A+G+SGED
Sbjct: 1039 HAMMVLRKL-------FGGDLVPDPVASV------VTDWGTDPYSYGAYSYVAIGASGED 1098

BLAST of CmoCh12G013260 vs. TAIR 10
Match: AT3G13682.1 (LSD1-like2 )

HSP 1 Score: 92.4 bits (228), Expect = 1.0e-18
Identity = 134/515 (26.02%), Postives = 211/515 (40.97%), Query Frame = 0

Query: 5   KAKIVIIGAGMAGLTAANRLYT-------APGSADLFDISVVEFMGQK-----IEMGATW 64
           +  ++++GAG+AGL AA +L +         G +        + MG K     +E+G + 
Sbjct: 159 EGSVIVVGAGLAGLAAARQLLSFGFKVLVLEGRSRPGGRVYTQKMGGKDRFAAVELGGSV 218

Query: 65  IHGIRGSPIHEIADQIN-ALHSDQP-WEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLFT 124
           I G+  +P+  +A Q++  LH  +      N+       VA+  VE            F 
Sbjct: 219 ITGLHANPLGVLARQLSIPLHKVRDNCPLYNSEGVLVDKVADSNVEFG----------FN 278

Query: 125 NLIDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECKGWNQKSLE 184
            L+D    K+    + +  A + K+S+G  L      Y ++K+   E  +   W+  +LE
Sbjct: 279 KLLD----KVTEVREMMEGA-AKKISLGEVLETLRVLYGVAKDS--EERKLFDWHLANLE 338

Query: 185 EAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFPGDEITIPKGYSTVIQSIASILPPGL 244
            A      N+   Y             +  Y++  GD   +  G   +I ++A  LP   
Sbjct: 339 YANAGCLSNLSAAYWD----------QDDPYEM-GGDHCFLAGGNWRLINALAEGLP--- 398

Query: 245 VQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDI-SADHVIVTVSLVVLKAGTQQESEL 304
           +   K+V  I++              +    GS I  AD ++ TV L VLK         
Sbjct: 399 IIYGKSVDTIKYGDG----------GVEVISGSQIFQADMILCTVPLGVLKK-------- 458

Query: 305 KSINKSPCLNFVFHRPDSEFRRKKIPWWIRKTSFLRPIYQNSSILLSWFAGEEALHLGKL 364
           +SI   P L           RRK+    I +  F   + + + +  S F G+E    G L
Sbjct: 459 RSIKFEPELP----------RRKQAA--IDRLGF-GLLNKVAMLFPSVFWGDELDTFGCL 518

Query: 365 NEEEVING------VSTTISN-----FQIPNSESDCNDCEANSKGSVKFSKVLK------ 424
           NE  +  G         T+S        +    +   +C   ++ SV   +VLK      
Sbjct: 519 NESSINRGEFFLFYAYHTVSGGPALVALVAGEAAQRFEC---TEPSVLLHRVLKKLRGIY 578

Query: 425 ---------------SQWGSDPLFQGSYSYIAVGSSGEDLDAMAEPLPRTEESPKAPLLQ 473
                          ++WGSDPL  GSYS++ VGSSG D D +AE +            +
Sbjct: 579 GPKGVVVPDPIQTVCTRWGSDPLSYGSYSHVRVGSSGVDYDILAESVSN----------R 598

BLAST of CmoCh12G013260 vs. TAIR 10
Match: AT3G10390.1 (Flavin containing amine oxidoreductase family protein )

HSP 1 Score: 80.9 bits (198), Expect = 3.1e-15
Identity = 112/499 (22.44%), Postives = 194/499 (38.88%), Query Frame = 0

Query: 5   KAKIVIIGAGMAGLTAANRLY------------TAPGSADLFDISVVEFMGQKIEMGATW 64
           K+ ++I+GAG++GL AA +L               PG            +G   ++G + 
Sbjct: 184 KSSVIIVGAGLSGLAAARQLMRFGFKVTVLEGRKRPGGRVYTKKMEANRVGAAADLGGSV 243

Query: 65  IHGIRGSPIHEIADQINALHSDQPWEFMNAWSAHSTTVAEGGVEISPAIIDPVSSLFTNL 124
           + G  G+P+  IA Q+ +      ++  +    +       G  + P +   V   F  L
Sbjct: 244 LTGTLGNPLGIIARQLGS----SLYKVRDKCPLYRV----DGKPVDPDVDIKVEVAFNQL 303

Query: 125 IDFSQGKIAGDSDFLRQAKSDKLSIGAYLRRGLDSYLISKNGEIEFNECK--GWNQKSLE 184
           +D         +  LRQ   D +S+   L   L+++      ++   E     W+  +LE
Sbjct: 304 LD--------KASKLRQLMGD-VSMDVSLGAALETFRQVSGNDVATEEMGLFNWHLANLE 363

Query: 185 EAIFAMYENIQRTYTSAGELSTVDLVAESEYQVFP--GDEITIPKGYSTVIQSIASILPP 244
                        Y +AG +S + L    +   +   GD   +P G   ++Q++A  +P 
Sbjct: 364 -------------YANAGLVSKLSLAFWDQDDPYDMGGDHCFLPGGNGRLVQALAENVP- 423

Query: 245 GLVQLNKNVTKIEWHPEPDARNVPTPVRIHFSDGSDI-SADHVIVTVSLVVLKAGTQQES 304
             +   K V  I +              +  + G+ +   D V+ TV L VLK G+ +  
Sbjct: 424 --ILYEKTVQTIRYGSN----------GVKVTAGNQVYEGDMVLCTVPLGVLKNGSIKFV 483

Query: 305 ELKSINKSPCLN---------------FVFHRPDSE-FRRKKIPWWIRKTSFLRPIY--- 364
                 K  C+                +VF   D + F         R   FL   Y   
Sbjct: 484 PELPQRKLDCIKRLGFGLLNKVAMLFPYVFWSTDLDTFGHLTEDPNYRGEFFLFYSYAPV 543

Query: 365 QNSSILLSWFAGEEALHLGKLNEEEVINGVSTTISNFQIPNSESDCNDCEANSKGSVKFS 424
              ++L++  AGE A     +   + +  V   +     P   +  +  +          
Sbjct: 544 AGGALLIALVAGEAAHKFETMPPTDAVTRVLHILRGIYEPQGINVPDPLQ---------- 603

Query: 425 KVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRT 468
             + ++WG DP   GSYS +AVG+SG+D D +AE +            ++ FAGEAT R 
Sbjct: 604 -TVCTRWGGDPFSLGSYSNVAVGASGDDYDILAESVGDG---------RLFFAGEATTRR 619

BLAST of CmoCh12G013260 vs. TAIR 10
Match: AT1G62830.1 (LSD1-like 1 )

HSP 1 Score: 72.8 bits (177), Expect = 8.4e-13
Identity = 37/80 (46.25%), Postives = 51/80 (63.75%), Query Frame = 0

Query: 389 KVLKSQWGSDPLFQGSYSYIAVGSSGEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRT 448
           + L S+WG D    GSYSY+AVGSSG+D D +AE +            ++ FAGEAT+R 
Sbjct: 634 QALCSRWGQDKFSYGSYSYVAVGSSGDDYDILAESVGDG---------RVFFAGEATNRQ 693

Query: 449 HYSTTHGAYFSGLREAIRLL 469
           + +T HGA+ SG+REA  +L
Sbjct: 694 YPATMHGAFLSGMREAANIL 704

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SU794.5e-13650.83Probable polyamine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PAO5 PE=1 SV=1[more]
Q5NAI72.5e-8637.15Polyamine oxidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAO1 PE=1 SV=1[more]
Q99K826.0e-4828.85Spermine oxidase OS=Mus musculus OX=10090 GN=Smox PE=1 SV=1[more]
Q865R17.9e-4829.98Peroxisomal N(1)-acetyl-spermine/spermidine oxidase OS=Bos taurus OX=9913 GN=PAO... [more]
Q9NWM08.1e-4528.34Spermine oxidase OS=Homo sapiens OX=9606 GN=SMOX PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G2I51.6e-20570.09probable polyamine oxidase 5 OS=Cucurbita moschata OX=3662 GN=LOC111450217 PE=4 ... [more]
A0A6J1KFF81.8e-20469.72probable polyamine oxidase 5 OS=Cucurbita maxima OX=3661 GN=LOC111493815 PE=4 SV... [more]
A0A5A7UZX31.2e-20068.48Putative polyamine oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CB611.2e-20068.48probable polyamine oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103498503 PE=4 SV=1[more]
A0A0A0LFR41.0e-19967.60Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G0004... [more]
Match NameE-valueIdentityDescription
AT4G29720.13.2e-13750.83polyamine oxidase 5 [more]
AT4G16310.15.4e-2024.44LSD1-like 3 [more]
AT3G13682.11.0e-1826.02LSD1-like2 [more]
AT3G10390.13.1e-1522.44Flavin containing amine oxidoreductase family protein [more]
AT1G62830.18.4e-1346.25LSD1-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002937Amine oxidasePFAMPF01593Amino_oxidasecoord: 15..467
e-value: 2.9E-52
score: 178.5
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 1..100
e-value: 5.8E-11
score: 44.6
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 417..473
e-value: 2.9E-5
score: 26.1
coord: 164..288
e-value: 2.7E-9
score: 39.3
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 1..471
NoneNo IPR availableGENE3D3.90.660.10coord: 289..412
e-value: 5.5E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR10742FLAVIN MONOAMINE OXIDASEcoord: 1..286
NoneNo IPR availablePANTHERPTHR10742FLAVIN MONOAMINE OXIDASEcoord: 293..474
NoneNo IPR availablePANTHERPTHR10742:SF374POLYAMINE OXIDASE 5-RELATEDcoord: 293..474
NoneNo IPR availablePANTHERPTHR10742:SF374POLYAMINE OXIDASE 5-RELATEDcoord: 1..286
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 316..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G013260.1CmoCh12G013260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006598 polyamine catabolic process
molecular_function GO:0046592 polyamine oxidase activity
molecular_function GO:0016491 oxidoreductase activity