CmoCh12G013160 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G013160
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmitochondrial-processing peptidase subunit alpha-like
LocationCmo_Chr12: 11514055 .. 11517050 (-)
RNA-Seq ExpressionCmoCh12G013160
SyntenyCmoCh12G013160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAAGATAACAGCGTCGAAGTCTTTCGGCATATTGAGCTGCTAGGTTATTGATTTAATTGTAGTGACTTTTTATATCTCGAGTGTTAATAATATAGCGGCTATTTGTAACTATTCAATTCTTGTTGGTTTATTCTGTACGGCGATTCGGTTAATATTTGTATAAATGCTCGTCTTTGTCACTGTTTAAATCAGTGGAAACATGAACAATAGTTATAATTCTTCGAGTACTGTGCATGGTATAAGATCTTTGCAGAGACGTGCTTGTGTATTGAGCTTTTGAGTCTAACATGGCTTGAAATATGATTTTGACATCTACATAAAGACCTCGGGAATTAAATGCTTCGATGGATAATGCTGATAATTTTGCTTTCGAACATGAAACAATGGGCTGTATGGATTTGCCAGCTCTAGTGCTGTTGCCTCAAAAGTACAAATCTTCTGGGGGTGTCTTTGGCTGGTTGCTTCGAGACCGTTCTATATCATCTCCACTAGACTTTCCCTGTCGGATGTGAATCTTCCGTCTCCATTGCCAGATTATGTTGAACCTAGTGAGACGAAAATCTCAACTCTGCCAAATGGTGTAAGAGTAGCATCAGAAACATCACCGGTGCACCCATGTGCACCCATAGATTTGGTTTTTTCAAAATCTGTCCATGTGCACCCATATAATTAATGATTAATTACGTTTATTTATTAACATGCTGTTGGTTATTTTGGTGCCTTGGCAAATTCTCTTTTAGCCCCCGAGTCTGCTATAAATAGTCTGAGTGGTACAGTTTTGGAGAAATTTGTTGCTGTAAGTTGAGTAGGAGATCATAATATCATATTTATTTTTAGAATTAATTATTTGATGATGTTACTTCTTTATTGGTCATGCTTCTGCCTTCAGGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTTATCAAGGTGATTCTGGGGTGTGTATCATCTATATTGTATACTCTAGCATCCAGCCTTCATTTCCAGGCATCATTCGTTAACTGTTCTCTTTCAGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCAGGCGGCTGGCATAAGGAGAAGGATGCTATGGCCTCAACTGTTCTTCAGGTCTTAACAATGGATATCTATTGTGCTTAAGTGATTTTTATGACATGGGGGTTTAGATCATACACCATTAGTTGTTTAGTCCATTTTGCAATTTTTCACTGTGAAAAGACAAAGGCAATAATGTATCTCATCTCTTAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGGGGATGAGCTCTCGGCTGTGTAAGTCCTAAATTTCTTTGACTTTGACGGTCATGCATCTGAGATATTTTTCTTTTCTTGGGGTGGGGTTTGAAGGGAGGTGGAGGGTCTTTTTCAGCTGGTGGACCTGGAAAGGGGATGAGCTCTCGGCCGTGTAAGTCCTAAATTTCTTTGACTTTGACGGCCATACATCTGAGATATTTTTCTTTTCTTGGGTGGTGGGGGTGGGGTTTGAAGGGAGGAATTTGTATTTCCTATACTTGCATGTCAAAATATTGTCCTTCACGCCTGCCAAGTCAGGTCACATAGATAGTTTATTATGCAATTATTTCTAGACAGACATAACCTTCTTCTGCATGTATATTTCTTAATTTACGCATATTGTATGTTTTCTAGTGTTAACTTCTTTGTAATTATCGATGTGGTAAGCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTGTAATGATTCTCTTTGGATTGATATTTAACATGATATAATGCAGAATCACTATGTGGATCTTGATGGTAGAATTGTTAGGTTGATGATATACTTTTTTGAGGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGTCATTTGTGATTCTCTCTTCTCTAGCATTTCCACAAGAACCATTTCATCTAGCTCACTGATAACATTTCCCTGTTTACAGTTGAACAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTATGTATCATTGTACTTTCTTCTCGTTGGCCTTACCATGCCTATATCTTAGGTTGATGAAATAAAAATAATACAGTTTTGAAAATGTTAATCTTTGAGAATGTGTCCACTGATTGTGGTTGTGGAATTGTATCTTGTAGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGTACGGTCTGAATCATTTATTGTAGTCTTAATCTGATGCACCATGGCCCATTCATTTCTAATTCATTTCTCTTGTACTAGGAGGGTAAATCTATTTCAAAGATCTGAGATTTAGTTATGCTGTTTTCCCTCATTGAACTGCATTCTGTTACATGATCATTTGTTCACATTTTCTAAATGATATATATCATGCCTCCTGGTTACGCACTTACACTCTAGGGATTCTGGCCTTAGTTAAGAGTGTTAATTTCTTAAGATCTGTTAGAAGTTGCAAATATAATGTGAAGTGATTATCTTTGAATTTTGAGGTGAGCTTTGTAGTTACACCAGCGATCTGTCTTCAAGCCACGCATGCTCATTTTTAATGATCATTTGTTATGTCTTAAATATATAATGTATCCCATTATCCAGGAAACCCGTGGAACATGTCTTGAAAGCTGCGGACGCTGTAACTCTAGAGGATATAGCTTCAATTGCACAAAAACTTCTCTCTTCTCCATTGACAATCGCCTACTTATCATGCTCAATTAGAACCTTATGA

mRNA sequence

ATGGTGAAAGATAACAGCGTCGAAGTCTTTCGGCATATTGAGCTGCTAGTTTTGGAGAAATTTGTTGCTGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTTATCAAGGTGATTCTGGGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCAGGCGGCTGGCATAAGGAGAAGGATGCTATGGCCTCAACTGTTCTTCAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGGGGATGAGCTCTCGGCTGTCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGAAACCCGTGGAACATGTCTTGAAAGCTGCGGACGCTGTAACTCTAGAGGATATAGCTTCAATTGCACAAAAACTTCTCTCTTCTCCATTGACAATCGCCTACTTATCATGCTCAATTAGAACCTTATGA

Coding sequence (CDS)

ATGGTGAAAGATAACAGCGTCGAAGTCTTTCGGCATATTGAGCTGCTAGTTTTGGAGAAATTTGTTGCTGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTTATCAAGGTGATTCTGGGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCAGGCGGCTGGCATAAGGAGAAGGATGCTATGGCCTCAACTGTTCTTCAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGGGGATGAGCTCTCGGCTGTCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGAAACCCGTGGAACATGTCTTGAAAGCTGCGGACGCTGTAACTCTAGAGGATATAGCTTCAATTGCACAAAAACTTCTCTCTTCTCCATTGACAATCGCCTACTTATCATGCTCAATTAGAACCTTATGA

Protein sequence

MVKDNSVEVFRHIELLVLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGDSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDIASIAQKLLSSPLTIAYLSCSIRTL
Homology
BLAST of CmoCh12G013160 vs. ExPASy Swiss-Prot
Match: P29677 (Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN=MPP PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.3e-71
Identity = 153/256 (59.77%), Postives = 185/256 (72.27%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPR-EVPKSVYNGGDYRYQG 76
           VLE+FVAENYTA R++LAASGVEHEE L +AEPLLSDL  V   E PK VY GGDYR Q 
Sbjct: 241 VLEEFVAENYTAPRMVLAASGVEHEEFLKVAEPLLSDLPKVATIEEPKPVYVGGDYRCQA 300

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
           D+    THFALAFE+PGGW  EK++M  TVLQML+GGGGSFSAGGPGKGM SRL YL+VL
Sbjct: 301 DA--EMTHFALAFEVPGGWMSEKESMTLTVLQMLMGGGGSFSAGGPGKGMYSRL-YLRVL 360

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           N+YPQ+ + SAFSSIYNNTG+FGIQ TT S+F P++  +A  EL+A+A P          
Sbjct: 361 NQYPQIHAFSAFSSIYNNTGLFGIQGTTSSDFGPQAVDVAVKELIAVANPSEVDQVQLNR 420

Query: 197 -KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLED 256
            K A +S  ++N    + A+E           IG+ +LTYGE  PVEH LKA DAV+ +D
Sbjct: 421 AKQATKSAILMNLESRMVASE----------DIGRQLLTYGERNPVEHFLKAIDAVSAKD 480

Query: 257 IASIAQKLLSSPLTIA 260
           IAS+ QKL+SSPLT+A
Sbjct: 481 IASVVQKLISSPLTMA 483

BLAST of CmoCh12G013160 vs. ExPASy Swiss-Prot
Match: O04308 (Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha2 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 5.6e-59
Identity = 131/256 (51.17%), Postives = 173/256 (67.58%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRYQG 76
           VLE FV ENYTA+R++LAASGV+HEELL + EPLLSDL +VPR   PKS Y GGD+R   
Sbjct: 240 VLENFVFENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQH- 299

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
            +G   THFALAFE+P GW+ EK+A+ +TVLQML+GGGGSFSAGGPGKGM S L YL++L
Sbjct: 300 -TGGEATHFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWL-YLRLL 359

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           N++ Q QS +AF+S++NNTG+FGI   T   F  +   + ASE+ A+A  +         
Sbjct: 360 NQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVADGKVNQKHLDRA 419

Query: 197 KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLED 256
           K A +S  ++N    +I A +           IG+ +LTYGE KPV+  LK  D +TL+D
Sbjct: 420 KAATKSAILMNLESRMIAAED-----------IGRQILTYGERKPVDQFLKTVDQLTLKD 479

Query: 257 IASIAQKLLSSPLTIA 260
           IA    K+++ PLT+A
Sbjct: 480 IADFTSKVITKPLTMA 480

BLAST of CmoCh12G013160 vs. ExPASy Swiss-Prot
Match: Q9ZU25 (Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.1e-58
Identity = 129/255 (50.59%), Postives = 174/255 (68.24%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRYQG 76
           +LE+F+ EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R   
Sbjct: 244 LLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH- 303

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
            +G   THFA+AFE+P GW+ EK+A+ +TVLQML+GGGGSFSAGGPGKGM S L Y +VL
Sbjct: 304 -TGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVL 363

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           NEY +VQS +AF+SI+N+TG+FGI   +   F  K+  +AA EL  +A  +         
Sbjct: 364 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQAHLDRA 423

Query: 197 KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLED 256
           K A +S  ++N    +I A +           IG+ +LTYGE KPV+  LK+ D +TL+D
Sbjct: 424 KAATKSAVLMNLESRMIAAED-----------IGRQILTYGERKPVDQFLKSVDQLTLKD 483

Query: 257 IASIAQKLLSSPLTI 259
           IA    K++S PLT+
Sbjct: 484 IADFTSKVISKPLTM 483

BLAST of CmoCh12G013160 vs. ExPASy Swiss-Prot
Match: P97997 (Mitochondrial-processing peptidase subunit alpha OS=Blastocladiella emersonii OX=4808 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.3e-19
Identity = 77/257 (29.96%), Postives = 128/257 (49.81%), Query Frame = 0

Query: 18  LEKFVAENYTATRILLAASGVEHEELLS-IAEPLLSDLRSVPREVPKS----VYNGGDYR 77
           + ++ A     +R+++A +GV H EL+  +++  +      P  V  S     Y GG ++
Sbjct: 183 IREYFATYLHPSRMVVAGTGVAHAELVDLVSKAFVPSSTRAPSSVTHSDIETAYVGGSHQ 242

Query: 78  Y--------QGDSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKG 137
                      +     TH  +AF +P   H   D    + LQ+L+GGGG+FSAGGPGKG
Sbjct: 243 LVIPKPPPTHPNYEQTLTHVQVAFPVPPFTH--PDMFPVSTLQVLMGGGGAFSAGGPGKG 302

Query: 138 MSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIA- 197
           M SRL Y  VLN Y  ++S +AF   Y++T +FGI A+   +F P   ++ A E + +A 
Sbjct: 303 MYSRL-YTNVLNRYRWMESCAAFQHAYSSTSLFGISASCVPSFNPHLCNVLAGEFVHMAR 362

Query: 198 --TPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTL 257
             + E+ A    Q+ + L+   E  +        IG+ VL   +      ++    AVT 
Sbjct: 363 NLSDEEVARAKNQLKSSLLMNLESQV---ITVEDIGRQVLAQNQRLEPLELVNNISAVTR 422

Query: 258 EDIASIAQKLLSSPLTI 259
           +D+  +A+ L++ P T+
Sbjct: 423 DDLVRVAEALVAKPPTM 433

BLAST of CmoCh12G013160 vs. ExPASy Swiss-Prot
Match: P11914 (Mitochondrial-processing peptidase subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAS2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.1e-18
Identity = 77/242 (31.82%), Postives = 117/242 (48.35%), Query Frame = 0

Query: 26  YTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSV--YNGGDYRYQGDSGDGRT- 85
           YT    + A  GV HE+ L + E  L D +S    + K V  Y GG+         G   
Sbjct: 195 YTPENTVAAFVGVPHEKALELTEKYLGDWQSTHPPITKKVAQYTGGESCIPPAPVFGNLP 254

Query: 86  ---HFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLNEYP 145
              H  + FE  G      D  A   LQ LLGGGGSFSAGGPGKGM SRL Y  VLN+Y 
Sbjct: 255 ELFHIQIGFE--GLPIDHPDIYALATLQTLLGGGGSFSAGGPGKGMYSRL-YTHVLNQYY 314

Query: 146 QVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASEL--------LAIATPEKGAAR 205
            V++  AF+  Y+++GIFGI  +      P++  + A ++        L +   E   A+
Sbjct: 315 FVENCVAFNHSYSDSGIFGISLSCIPQAAPQAVEVIAQQMYNTFANKDLRLTEDEVSRAK 374

Query: 206 SCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDIASIAQKL 254
           + Q+ + L+   E  +        +G+ VL +G   PV  ++   + +  +DI+ +A+ +
Sbjct: 375 N-QLKSSLLMNLESKL---VELEDMGRQVLMHGRKIPVNEMISKIEDLKPDDISRVAEMI 429

BLAST of CmoCh12G013160 vs. ExPASy TrEMBL
Match: A0A6J1G486 (mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita moschata OX=3662 GN=LOC111450651 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 6.6e-95
Identity = 196/255 (76.86%), Postives = 212/255 (83.14%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGD 76
           +LEKFV+ENYTA+RI+LAASGVEHEELLSIAEPLLSDL SVP E PKSVYNGGDYR+QGD
Sbjct: 241 ILEKFVSENYTASRIVLAASGVEHEELLSIAEPLLSDLPSVPHEEPKSVYNGGDYRHQGD 300

Query: 77  SGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLN 136
           SGDGRTHFALAFELPGGWHKEKDAMA T+LQMLLGGGGSFSAGGPGKGM SRL YLQVLN
Sbjct: 301 SGDGRTHFALAFELPGGWHKEKDAMALTILQMLLGGGGSFSAGGPGKGMYSRL-YLQVLN 360

Query: 137 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATP----------E 196
           EYPQVQSISAFSSIYNN+GIFGIQATTGSNFVPK+F IAASELLAIATP           
Sbjct: 361 EYPQVQSISAFSSIYNNSGIFGIQATTGSNFVPKAFDIAASELLAIATPGKVEQVQLDRA 420

Query: 197 KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDI 256
           K + +S  ++N    + A+E           IG+ VLTYGE KPVEH LKA D VTLEDI
Sbjct: 421 KQSTKSTVLMNLESRVVASE----------DIGRQVLTYGERKPVEHFLKAVDEVTLEDI 480

Query: 257 ASIAQKLLSSPLTIA 260
           ASIAQKLLSSPLT+A
Sbjct: 481 ASIAQKLLSSPLTMA 484

BLAST of CmoCh12G013160 vs. ExPASy TrEMBL
Match: A0A6J1KD66 (mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita maxima OX=3661 GN=LOC111493827 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 3.0e-92
Identity = 192/255 (75.29%), Postives = 208/255 (81.57%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGD 76
           +LEKFV+ENYTA+R +LAASGVEHEELLSIAEPLLSDL S P E PKSVYNGGD R+QGD
Sbjct: 241 ILEKFVSENYTASRTVLAASGVEHEELLSIAEPLLSDLPSAPHEEPKSVYNGGDCRHQGD 300

Query: 77  SGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLN 136
           SGDGRTHFALAFELPGGWHKEKDAMA T+LQMLLGGGGSFSAGGPGKGM SRL YLQVLN
Sbjct: 301 SGDGRTHFALAFELPGGWHKEKDAMALTILQMLLGGGGSFSAGGPGKGMYSRL-YLQVLN 360

Query: 137 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATP----------E 196
           EYPQVQSISAFSSIYNN+GIFGIQATT SNFVPK+F IAASELLAIATP           
Sbjct: 361 EYPQVQSISAFSSIYNNSGIFGIQATTASNFVPKAFDIAASELLAIATPGKVEQVQLDRA 420

Query: 197 KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDI 256
           K + +S  ++N    + A+E           IG+ VLTYGE KPVEH LKA D VTLEDI
Sbjct: 421 KQSTKSAVLMNLESRVVASE----------DIGRQVLTYGERKPVEHFLKAVDEVTLEDI 480

Query: 257 ASIAQKLLSSPLTIA 260
           ASIAQKLLSSPLT+A
Sbjct: 481 ASIAQKLLSSPLTMA 484

BLAST of CmoCh12G013160 vs. ExPASy TrEMBL
Match: A0A6J1DL19 (mitochondrial-processing peptidase subunit alpha-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021899 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.2e-91
Identity = 190/255 (74.51%), Postives = 210/255 (82.35%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGD 76
           +LEKFV+ENY+A+RI+LAASGVEHEELLSIAEPLLSDL SVPRE PKSVYNGGDYR+QGD
Sbjct: 243 ILEKFVSENYSASRIVLAASGVEHEELLSIAEPLLSDLPSVPREEPKSVYNGGDYRHQGD 302

Query: 77  SGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLN 136
           SGDGRTHFALAFELPGGW KEKDAMA TVLQML+GGGGSFSAGGPGKGM SRL YLQVLN
Sbjct: 303 SGDGRTHFALAFELPGGWRKEKDAMALTVLQMLMGGGGSFSAGGPGKGMYSRL-YLQVLN 362

Query: 137 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATP----------E 196
           EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPK+F IAASELLAIA P           
Sbjct: 363 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKAFDIAASELLAIAMPGKVEQVQLDRA 422

Query: 197 KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDI 256
           K + +S  ++N    + A+E           IG+ VLTYGE KPVE+ LKA D +TL+DI
Sbjct: 423 KQSTKSAMLMNLESRVVASE----------DIGRQVLTYGERKPVEYFLKAVDELTLKDI 482

Query: 257 ASIAQKLLSSPLTIA 260
           AS AQ+LLSSPLT+A
Sbjct: 483 ASTAQRLLSSPLTMA 486

BLAST of CmoCh12G013160 vs. ExPASy TrEMBL
Match: A0A6J1DMY5 (mitochondrial-processing peptidase subunit alpha-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021899 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.2e-91
Identity = 190/255 (74.51%), Postives = 210/255 (82.35%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGD 76
           +LEKFV+ENY+A+RI+LAASGVEHEELLSIAEPLLSDL SVPRE PKSVYNGGDYR+QGD
Sbjct: 153 ILEKFVSENYSASRIVLAASGVEHEELLSIAEPLLSDLPSVPREEPKSVYNGGDYRHQGD 212

Query: 77  SGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLN 136
           SGDGRTHFALAFELPGGW KEKDAMA TVLQML+GGGGSFSAGGPGKGM SRL YLQVLN
Sbjct: 213 SGDGRTHFALAFELPGGWRKEKDAMALTVLQMLMGGGGSFSAGGPGKGMYSRL-YLQVLN 272

Query: 137 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATP----------E 196
           EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPK+F IAASELLAIA P           
Sbjct: 273 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKAFDIAASELLAIAMPGKVEQVQLDRA 332

Query: 197 KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDI 256
           K + +S  ++N    + A+E           IG+ VLTYGE KPVE+ LKA D +TL+DI
Sbjct: 333 KQSTKSAMLMNLESRVVASE----------DIGRQVLTYGERKPVEYFLKAVDELTLKDI 392

Query: 257 ASIAQKLLSSPLTIA 260
           AS AQ+LLSSPLT+A
Sbjct: 393 ASTAQRLLSSPLTMA 396

BLAST of CmoCh12G013160 vs. ExPASy TrEMBL
Match: A0A5D3CG40 (Mitochondrial-processing peptidase subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00740 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 6.4e-90
Identity = 186/255 (72.94%), Postives = 207/255 (81.18%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRYQGD 76
           +LE FV+ENYTA+RI+LAASGVEHEELLSIAEPLLSDL  VP + PKSVYNGGDYR+QGD
Sbjct: 229 ILENFVSENYTASRIVLAASGVEHEELLSIAEPLLSDLPGVPHQEPKSVYNGGDYRHQGD 288

Query: 77  SGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVLN 136
           SGDGRTHFALAFELPGGW KEKDAMA TVLQMLLGGGGSFSAGGPGKGM SRL YLQVLN
Sbjct: 289 SGDGRTHFALAFELPGGWRKEKDAMALTVLQMLLGGGGSFSAGGPGKGMYSRL-YLQVLN 348

Query: 137 EYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATP----------E 196
           EYPQVQSISAFSSIYNN+G+FGI+ TTGS+FVPK+F IAASELLAIATP           
Sbjct: 349 EYPQVQSISAFSSIYNNSGLFGIKGTTGSDFVPKAFDIAASELLAIATPGKVQQVQLDRA 408

Query: 197 KGAARSCQIVN--ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDI 256
           K + +S  ++N    + A+E           I + VLTYGE KPVEH LKA D VTL+D+
Sbjct: 409 KQSTKSAVLMNLESRVVASE----------DIARQVLTYGERKPVEHFLKAVDEVTLDDV 468

Query: 257 ASIAQKLLSSPLTIA 260
           ASIAQKLLSSPLT+A
Sbjct: 469 ASIAQKLLSSPLTMA 472

BLAST of CmoCh12G013160 vs. TAIR 10
Match: AT3G16480.1 (mitochondrial processing peptidase alpha subunit )

HSP 1 Score: 229.2 bits (583), Expect = 4.0e-60
Identity = 131/256 (51.17%), Postives = 173/256 (67.58%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRYQG 76
           VLE FV ENYTA+R++LAASGV+HEELL + EPLLSDL +VPR   PKS Y GGD+R   
Sbjct: 240 VLENFVFENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQH- 299

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
            +G   THFALAFE+P GW+ EK+A+ +TVLQML+GGGGSFSAGGPGKGM S L YL++L
Sbjct: 300 -TGGEATHFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWL-YLRLL 359

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           N++ Q QS +AF+S++NNTG+FGI   T   F  +   + ASE+ A+A  +         
Sbjct: 360 NQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVADGKVNQKHLDRA 419

Query: 197 KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLED 256
           K A +S  ++N    +I A +           IG+ +LTYGE KPV+  LK  D +TL+D
Sbjct: 420 KAATKSAILMNLESRMIAAED-----------IGRQILTYGERKPVDQFLKTVDQLTLKD 479

Query: 257 IASIAQKLLSSPLTIA 260
           IA    K+++ PLT+A
Sbjct: 480 IADFTSKVITKPLTMA 480

BLAST of CmoCh12G013160 vs. TAIR 10
Match: AT1G51980.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 225.3 bits (573), Expect = 5.7e-59
Identity = 129/255 (50.59%), Postives = 174/255 (68.24%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRYQG 76
           +LE+F+ EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R   
Sbjct: 244 LLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH- 303

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
            +G   THFA+AFE+P GW+ EK+A+ +TVLQML+GGGGSFSAGGPGKGM S L Y +VL
Sbjct: 304 -TGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVL 363

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           NEY +VQS +AF+SI+N+TG+FGI   +   F  K+  +AA EL  +A  +         
Sbjct: 364 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQAHLDRA 423

Query: 197 KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLED 256
           K A +S  ++N    +I A +           IG+ +LTYGE KPV+  LK+ D +TL+D
Sbjct: 424 KAATKSAVLMNLESRMIAAED-----------IGRQILTYGERKPVDQFLKSVDQLTLKD 483

Query: 257 IASIAQKLLSSPLTI 259
           IA    K++S PLT+
Sbjct: 484 IADFTSKVISKPLTM 483

BLAST of CmoCh12G013160 vs. TAIR 10
Match: AT1G51980.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 194.9 bits (494), Expect = 8.3e-50
Identity = 113/222 (50.90%), Postives = 151/222 (68.02%), Query Frame = 0

Query: 17  VLEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRYQG 76
           +LE+F+ EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R   
Sbjct: 244 LLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH- 303

Query: 77  DSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLSYLQVL 136
            +G   THFA+AFE+P GW+ EK+A+ +TVLQML+GGGGSFSAGGPGKGM S L Y +VL
Sbjct: 304 -TGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVL 363

Query: 137 NEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASELLAIATPE--------- 196
           NEY +VQS +AF+SI+N+TG+FGI   +   F  K+  +AA EL  +A  +         
Sbjct: 364 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQAHLDRA 423

Query: 197 KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGE 226
           K A +S  ++N    +I A +           IG+ +LTYGE
Sbjct: 424 KAATKSAVLMNLESRMIAAED-----------IGRQILTYGE 450

BLAST of CmoCh12G013160 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 72.0 bits (175), Expect = 8.1e-13
Identity = 68/244 (27.87%), Postives = 116/244 (47.54%), Query Frame = 0

Query: 18  LEKFVAENYTATRILLAASG-VEHEELLSIAEPLLSDLRSVPREVPKSVYN------GGD 77
           L+ ++  +YTA+R+++AA+G V+HEE++   + L + L S P    + V N      G +
Sbjct: 266 LQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSE 325

Query: 78  YRYQGDSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLS 137
            R   D       FA+AFE  G    + D++A  V+Q +LG     + GG GK + S L+
Sbjct: 326 VRMIDDDLP-LAQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLT 385

Query: 138 YLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASEL--LAIATPEKG 197
               +NE    +SI AF++ Y +TG+FG+ A   ++ +    +    E+  LA    +  
Sbjct: 386 QRVAINEI--AESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDAD 445

Query: 198 AARSCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDIASIA 253
             R+   +   +  +  G         IG+ +LTYG   P   +    DAV    +  +A
Sbjct: 446 VTRARNQLKSSLLLHMDGTSP--IAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVA 500

BLAST of CmoCh12G013160 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 72.0 bits (175), Expect = 8.1e-13
Identity = 68/244 (27.87%), Postives = 116/244 (47.54%), Query Frame = 0

Query: 18  LEKFVAENYTATRILLAASG-VEHEELLSIAEPLLSDLRSVPREVPKSVYN------GGD 77
           L+ ++  +YTA+R+++AA+G V+HEE++   + L + L S P    + V N      G +
Sbjct: 266 LQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSE 325

Query: 78  YRYQGDSGDGRTHFALAFELPGGWHKEKDAMASTVLQMLLGGGGSFSAGGPGKGMSSRLS 137
            R   D       FA+AFE  G    + D++A  V+Q +LG     + GG GK + S L+
Sbjct: 326 VRMIDDDLP-LAQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLT 385

Query: 138 YLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTGSNFVPKSFHIAASEL--LAIATPEKG 197
               +NE    +SI AF++ Y +TG+FG+ A   ++ +    +    E+  LA    +  
Sbjct: 386 QRVAINEI--AESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDAD 445

Query: 198 AARSCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHVLKAADAVTLEDIASIA 253
             R+   +   +  +  G         IG+ +LTYG   P   +    DAV    +  +A
Sbjct: 446 VTRARNQLKSSLLLHMDGTSP--IAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVA 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P296771.3e-7159.77Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN... [more]
O043085.6e-5951.17Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitoc... [more]
Q9ZU258.1e-5850.59Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Ar... [more]
P979973.3e-1929.96Mitochondrial-processing peptidase subunit alpha OS=Blastocladiella emersonii OX... [more]
P119142.1e-1831.82Mitochondrial-processing peptidase subunit alpha OS=Saccharomyces cerevisiae (st... [more]
Match NameE-valueIdentityDescription
A0A6J1G4866.6e-9576.86mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita moschata OX=3... [more]
A0A6J1KD663.0e-9275.29mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita maxima OX=366... [more]
A0A6J1DL191.2e-9174.51mitochondrial-processing peptidase subunit alpha-like isoform X1 OS=Momordica ch... [more]
A0A6J1DMY51.2e-9174.51mitochondrial-processing peptidase subunit alpha-like isoform X2 OS=Momordica ch... [more]
A0A5D3CG406.4e-9072.94Mitochondrial-processing peptidase subunit alpha-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT3G16480.14.0e-6051.17mitochondrial processing peptidase alpha subunit [more]
AT1G51980.15.7e-5950.59Insulinase (Peptidase family M16) protein [more]
AT1G51980.28.3e-5050.90Insulinase (Peptidase family M16) protein [more]
AT3G02090.18.1e-1327.87Insulinase (Peptidase family M16) protein [more]
AT3G02090.28.1e-1327.87Insulinase (Peptidase family M16) protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007863Peptidase M16, C-terminalPFAMPF05193Peptidase_M16_Ccoord: 16..184
e-value: 2.7E-21
score: 76.4
NoneNo IPR availableGENE3D3.30.830.10coord: 64..265
e-value: 3.1E-37
score: 130.1
NoneNo IPR availablePANTHERPTHR11851METALLOPROTEASEcoord: 15..260
NoneNo IPR availablePANTHERPTHR11851:SF206PROCESSING PEPTIDASEcoord: 15..260
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 66..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G013160.1CmoCh12G013160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity