CmoCh12G011060 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G011060
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr12: 10092689 .. 10096719 (-)
RNA-Seq ExpressionCmoCh12G011060
SyntenyCmoCh12G011060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCTTCCATCTTTCTTCGTCCCGTCGTTATTGTTCCTCTTGTCTTTTTGCTTACTGCACACTGAACTACTGACCCACCATTACCAACCATACCTTCAAAGCCATGATTTCCTCTACACACCCATTTCTTCTTCTTCTCCTTCATTTGCCCTTATAATCCAATGCCGCACTCAAATTCTACTCCCCTTCCACCACCGTCCATGGCGGCCCTCACCCACCTCCTCCTCCTCCTCCTCCTCTGCGTTTCCGCCGCCACATCCAACCCTTTCTCCAGCCCCGCGGTTTTCAACTTCGGCGACTCCAACTCCGACACCGGTTGCGTTGTCGGTGCTGCCATTGAGAACATCTACCCCCCCTACGGCCACCGCTTCTTCGGACATCCCTCTGGGCGATACTCCGATGGCCGCCTCATCGTTGATTTTCTCTGTACGTTTCTGTTCTTCTTGGATCCTTTTTTCTCTCTGGAATTGATTTTCTTTTCTGCGATTAGGGTTTTCAGTTTTCGTCTCTCCAATCTAGCATACAATCTCCATTGTTATTAGGCTTTTTGGGGAAGTCCAAAGCAAAGCAGGAGAGATTAGGCTCAAAGTGAACAATATCATACCATTGTGGAGAGTCGTGGAGAGTCGTATTTGTCAATAGAATCCTCAAATGTAGAACAAGGTAGGTGAGCCTCGAAGGCTTAACTAAGACTCCAAAAAGAAAAAGGAGTCGAGCCATTAAGGGAAGACGTATTTTGTTCAAGGAGAGGTGTTTGATGAAAGTCCCACATTGGGTCATCGGCTAATTTTAGGGAATGATCATATCCATTGGTATGAGGCTTTAGAGATTAGACTCAAAGTGAACAATATCATACCATTGTGAAGAATCGTGTTCATCTAACTTGGTACCAGAGTCATGCCCTAAACTTAGTCATGCCAATAGAATCCTCAAATGTCGAACAAAGTATTGTGAGCCTCGAAGGCTTGACTAAGACTCCGTAAAGAAAAAGGAGTCGAGCCTTGATTAAGGAGAGACATACTTTGTTCAAGGGGAGGTGTTGGATGAAAGTCCCACATTGGCTCATCGGCTAATTTAGGGAATGATCATACTATCTCTATTGGTACGAGGCCTTTTGAGAATCCCAAAGCAAAGCTACGAGAGTTTATGCTCAAAGTAGACAATATCATACCATTGTGGAGAGTCGTGTTCATGTAACCGTTTTCTCTCTTAGTTTGATGCTTAGATTCTCGTTGGTTTGCAATGTTTTTGCAGTGGATGCGATGGATATGCCTTACTTGAATGCGTATCTTGATTCCCTTGGCGCACCAAATTTTCGTAAGGGGTGCAATTATGCGGCTGCAGGCTCGACTATTCTTCCCGCAACAGCTACTTCTTTTTGCCCTTTTTCATTTGGGGTTCAGGTGAATCAGTTTCTTCATTTCAAAGCTAAAGTTCTTGAGCTTCGAGCAGGCAAAGGTATGATAGATTGTGAATGATCTCAAGAACACAACCATTTTCACTGTTTTGTGATCATTTCTGCTGCTTTCTTTAGGTGGTAAGAAACTTGATAAGTACCTACCAGCTGATGATTACTTCCAGAAGGGGCTTTACATGTTTGATATTGGGCAGAATGATCTTGCTGGTGCATTTTATTCCAAAACTTTGGACCAAATTCTTGCCTCAATACCTGCAATTTTATCTGAATTTGAGAGTGGAGTTCAGGTTAGACTCAATGGCTTCTTTCGAAGTTATGTTGGCACAATGATGATTTTGTTTAGATGTGCTTTTACCTGCATGATTTCGTGTTAGATTTGGGTATTGAACTTGATTTTGAGTGTTTTGGAAGCTAGTTTTTGTATGATTTGTTGGTGGCTGATGATGGCTTTAGTTATAAACGATCATTTTTGTTAGAAGCATTTAGGTACTGAAGCGATCCAAGCCCACCGCTAGTAGATATTATTCTCTTTGGGTTTTTCCTTTTGGGTTTCCTTTAGGTTTTTAAAACGTGTTTGCTAGGGAGAGGTTTCTACACCCTTGTAAAGAATGTTTCGTTCCCCTCTCCAATCGATGTGGGATATCATAATCCACCCCCTTCAGGGTGCTGCGTATTCGCTCGCACTCGTTCCCTTCTCTAATTGATGTGGGACCCCAATCCACCTCCTTCGGAGCTTAGCCTCCTCGCTGCATATCGCTCGGTGTCTGTCTTTGATACCATTTGTAATGGCCCAAGCCCACCGCTAGCAAATATTGTCCTCTTTGGGCTTTCCCTTCCGAGCTTCTCCTCGCTGGCAATCATTTTGTTAGATACTGAAACGATCCAAGCCCACCGCTAGTAAATATTGTTTTCTTTGGACTTTCCTTTCGGGTTTCCCTTCAGGTTTTTAAAACGTGTCTGCTAGAGAGAGGTTTTCACACCCTTGTAAAGAATGTTTCGCTCCCCTCTTCAATTGATGTGTGATCTCACAATCCACCCCCTTTGAGGCCCAGCATCCTCATTGGCACTCGTTCCCTTCTCTAATTGATGTGAGACTCCCAATCCACCACCCCTTTCGGGGCCCAGCGTCCTTACTGGCACACCACCCCGTGTCCACCTCCTTCGGGGCTTAGCCTCCTCGCTGACACATCGCCCGGTGTCTGGCTCTGATACCATTTCTAACGGCCCAAGCCTACCGCTAGCAGATATTGTCCTCCTTGGGATTTACCTTTCAAGTTCTTCCTCGAGGCTTTTAAAACGCGTTTGCTAGTAAGAGGTTTCCACACCCTTATAAAGAATGCTTAGTTCCCTTCTATAAGTGATGTGAGATCTCACCGTTACTATTTCTGAACACATCTAGTGGTCGCGGGTGGCTACTTATTTAGGACACTACAACATTCGCCTCTTAAAATGTTAGAGTTGTGGTTGTTCTATAAGATTAATCGAAGTGAATGAAAGTAGGGTCCGACGCTAGCTGGATACAATACCCTCGTGTTGGTCAAAACTTAACTCGGGTATCACCAAGTAAAATACAGGGAAAACATAACGGGACGAGTTCTTTTACTATATTCTGTAGGATAAGACTCCATGGTTGTATTATCAATAAGGGTTAGATGAAACGAGATTAAATAATGATGGCTATCTACGAGTTTCCTTAGCAATCCAAATGAACTAGAGTCGGGTGGTTGTCTCGTGAGAACAGTCGAGGTGTGCATAATCCCGTTCGGTCATTACTTCATCATTCTTTTTGCCTATTTTTCAGAAACTGTACGACGAAGGGGCGAGAAATTTTTGGATTCACAACACGGGTCCTTTAGGATGCTTGGCTCAGAACGTTGCTAAATTTGGAACAGACCCATCAAAGCTTGATGAATTTGGATGCGTCAGTTCACACAACCAAGCCGCCAAACTCTTCAATCTACAGCTTCATGCTCTCTGTAAAAAACTGCAGGGCCAATATTCAGATGGCAACGTCACATACGTCGATATCTACTCGATAAAATCGAATCTCATCGCCAATTATTCACAACTCGGTTGGCCGCCTTGCTGTACTTTAGCTTCATTTTCTGTCAATCCTGTAACATCTGATGACTTGCTGCATTTTGACATGGCAGGTTTTGAGCAACCTATTATGGCTTGTTGTGGCTATGGAGGTCCACCGCTCAATTACGACAGTCGAATCGTGTGCGGACACACGAAGATGTTAAACGGGACGTTGGTGACGGCGAAAGGATGCGACGATAGCTCGGAGTACATCAATTGGGACGGAATTCATTATACAGAAGCTGCAAATCAGTATGTTTCATCACAAATACTCACCGGAAAATATTCTGATCCACCCTTCTCAGAAAAAATGCCATTCCTTCTCAAACTCAAGTTCTAGTTGGCCTTACTTACACATGCATACATCTCACAACTGATAACTCATTTATATGTCTAAATGGAAGCATTCATGTGGAATTTAGAACTATGATATGATATTAAGCGCTTTAAATGAAAAGGCCGAAACAGACACAGATGTGATACCATGAAGATTTTTCATAATAAAGCACGACGATAATGAATTAACCGAGCA

mRNA sequence

ATCCTTCCATCTTTCTTCGTCCCGTCGTTATTGTTCCTCTTGTCTTTTTGCTTACTGCACACTGAACTACTGACCCACCATTACCAACCATACCTTCAAAGCCATGATTTCCTCTACACACCCATTTCTTCTTCTTCTCCTTCATTTGCCCTTATAATCCAATGCCGCACTCAAATTCTACTCCCCTTCCACCACCGTCCATGGCGGCCCTCACCCACCTCCTCCTCCTCCTCCTCCTCTGCGTTTCCGCCGCCACATCCAACCCTTTCTCCAGCCCCGCGGTTTTCAACTTCGGCGACTCCAACTCCGACACCGGTTGCGTTGTCGGTGCTGCCATTGAGAACATCTACCCCCCCTACGGCCACCGCTTCTTCGGACATCCCTCTGGGCGATACTCCGATGGCCGCCTCATCGTTGATTTTCTCTTGGATGCGATGGATATGCCTTACTTGAATGCGTATCTTGATTCCCTTGGCGCACCAAATTTTCGTAAGGGGTGCAATTATGCGGCTGCAGGCTCGACTATTCTTCCCGCAACAGCTACTTCTTTTTGCCCTTTTTCATTTGGGGTTCAGGTGAATCAGTTTCTTCATTTCAAAGCTAAAGTTCTTGAGCTTCGAGCAGGCAAAGGTGGTAAGAAACTTGATAAGTACCTACCAGCTGATGATTACTTCCAGAAGGGGCTTTACATGTTTGATATTGGGCAGAATGATCTTGCTGGTGCATTTTATTCCAAAACTTTGGACCAAATTCTTGCCTCAATACCTGCAATTTTATCTGAATTTGAGAGTGGAGTTCAGAAACTGTACGACGAAGGGGCGAGAAATTTTTGGATTCACAACACGGGTCCTTTAGGATGCTTGGCTCAGAACGTTGCTAAATTTGGAACAGACCCATCAAAGCTTGATGAATTTGGATGCGTCAGTTCACACAACCAAGCCGCCAAACTCTTCAATCTACAGCTTCATGCTCTCTGTAAAAAACTGCAGGGCCAATATTCAGATGGCAACGTCACATACGTCGATATCTACTCGATAAAATCGAATCTCATCGCCAATTATTCACAACTCGGTTGGCCGCCTTGCTGTACTTTAGCTTCATTTTCTGTCAATCCTGTAACATCTGATGACTTGCTGCATTTTGACATGGCAGGTTTTGAGCAACCTATTATGGCTTGTTGTGGCTATGGAGGTCCACCGCTCAATTACGACAGTCGAATCGTGTGCGGACACACGAAGATGTTAAACGGGACGTTGGTGACGGCGAAAGGATGCGACGATAGCTCGGAGTACATCAATTGGGACGGAATTCATTATACAGAAGCTGCAAATCAGTATGTTTCATCACAAATACTCACCGGAAAATATTCTGATCCACCCTTCTCAGAAAAAATGCCATTCCTTCTCAAACTCAAGTTCTAGTTGGCCTTACTTACACATGCATACATCTCACAACTGATAACTCATTTATATGTCTAAATGGAAGCATTCATGTGGAATTTAGAACTATGATATGATATTAAGCGCTTTAAATGAAAAGGCCGAAACAGACACAGATGTGATACCATGAAGATTTTTCATAATAAAGCACGACGATAATGAATTAACCGAGCA

Coding sequence (CDS)

ATGCCGCACTCAAATTCTACTCCCCTTCCACCACCGTCCATGGCGGCCCTCACCCACCTCCTCCTCCTCCTCCTCCTCTGCGTTTCCGCCGCCACATCCAACCCTTTCTCCAGCCCCGCGGTTTTCAACTTCGGCGACTCCAACTCCGACACCGGTTGCGTTGTCGGTGCTGCCATTGAGAACATCTACCCCCCCTACGGCCACCGCTTCTTCGGACATCCCTCTGGGCGATACTCCGATGGCCGCCTCATCGTTGATTTTCTCTTGGATGCGATGGATATGCCTTACTTGAATGCGTATCTTGATTCCCTTGGCGCACCAAATTTTCGTAAGGGGTGCAATTATGCGGCTGCAGGCTCGACTATTCTTCCCGCAACAGCTACTTCTTTTTGCCCTTTTTCATTTGGGGTTCAGGTGAATCAGTTTCTTCATTTCAAAGCTAAAGTTCTTGAGCTTCGAGCAGGCAAAGGTGGTAAGAAACTTGATAAGTACCTACCAGCTGATGATTACTTCCAGAAGGGGCTTTACATGTTTGATATTGGGCAGAATGATCTTGCTGGTGCATTTTATTCCAAAACTTTGGACCAAATTCTTGCCTCAATACCTGCAATTTTATCTGAATTTGAGAGTGGAGTTCAGAAACTGTACGACGAAGGGGCGAGAAATTTTTGGATTCACAACACGGGTCCTTTAGGATGCTTGGCTCAGAACGTTGCTAAATTTGGAACAGACCCATCAAAGCTTGATGAATTTGGATGCGTCAGTTCACACAACCAAGCCGCCAAACTCTTCAATCTACAGCTTCATGCTCTCTGTAAAAAACTGCAGGGCCAATATTCAGATGGCAACGTCACATACGTCGATATCTACTCGATAAAATCGAATCTCATCGCCAATTATTCACAACTCGGTTGGCCGCCTTGCTGTACTTTAGCTTCATTTTCTGTCAATCCTGTAACATCTGATGACTTGCTGCATTTTGACATGGCAGGTTTTGAGCAACCTATTATGGCTTGTTGTGGCTATGGAGGTCCACCGCTCAATTACGACAGTCGAATCGTGTGCGGACACACGAAGATGTTAAACGGGACGTTGGTGACGGCGAAAGGATGCGACGATAGCTCGGAGTACATCAATTGGGACGGAATTCATTATACAGAAGCTGCAAATCAGTATGTTTCATCACAAATACTCACCGGAAAATATTCTGATCCACCCTTCTCAGAAAAAATGCCATTCCTTCTCAAACTCAAGTTCTAG

Protein sequence

MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF
Homology
BLAST of CmoCh12G011060 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.0e-152
Identity = 260/404 (64.36%), Postives = 316/404 (78.22%), Query Frame = 0

Query: 17  LTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSG 76
           L +++L  +  +  + S  F+ P+ FNFGDSNSDTG +V      +  P G   F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 77  RYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFG 136
           R+ DGRL++DFL+D MD+P+LN YLDSLG PNF+KGCN+AAAGSTILPA  TS  PFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 137 VQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 196
           +Q++QF+ FK++ +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIEL-LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 197 ILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSS 256
           +LASIP+IL  FE+G+++LY+EG RN WIHNTGPLGCLAQN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 257 HNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFS- 316
           HNQAAKLFNLQLHA+  K Q QY D NVTYVDI+SIKSNLIANYS+ G      L   + 
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNH 307

Query: 317 VNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSS 376
           +  V  + +L  ++ GFE+P+MACCG GG PLNYDSRI CG TK+L+G  VTAK C+DSS
Sbjct: 308 LENVGYNKIL--NVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSS 367

Query: 377 EYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           EYINWDGIHYTEAAN++VSSQILTGKYSDPPFS++MPF L LKF
Sbjct: 368 EYINWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 408

BLAST of CmoCh12G011060 vs. ExPASy Swiss-Prot
Match: Q9MAA1 (GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 4.3e-122
Identity = 225/401 (56.11%), Postives = 278/401 (69.33%), Query Frame = 0

Query: 14  MAALTHLLLLLLLCVSAA-TSNPFSS--------PAVFNFGDSNSDTGCVVGAAIENIYP 73
           M  L H LL LLL V+ + T +P +         PAVFNFGDSNSDTG +         P
Sbjct: 1   METLFHTLLRLLLFVAISHTLSPLAGSFRISNDFPAVFNFGDSNSDTGELSSGLGFLPQP 60

Query: 74  PYGHRFFGHP-SGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTIL 133
            Y   FF  P SGR+ +GRLIVDFL++A+D PYL  YLDS+    +R+GCN+AAA STI 
Sbjct: 61  SYEITFFRSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAASTIQ 120

Query: 134 PATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQN 193
            A A S+ PF FGVQV+QF+ FK+KVL+L   +  ++L +YLP++ +F  GLYMFDIGQN
Sbjct: 121 KANAASYSPFGFGVQVSQFITFKSKVLQLI--QQDEELQRYLPSEYFFSNGLYMFDIGQN 180

Query: 194 DLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGT 253
           D+AGAFY+KT+DQ+LA +P IL  F+ G+++LY EGARN+WIHNTGPLGCLAQ V+ FG 
Sbjct: 181 DIAGAFYTKTVDQVLALVPIILDIFQDGIKRLYAEGARNYWIHNTGPLGCLAQVVSIFGE 240

Query: 254 DPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQL 313
           D SKLDEFGCVS HNQAAKLFNLQLH L KKL  QY +   TYVDI+SIKS+LI N+S+ 
Sbjct: 241 DKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIFSIKSDLILNHSKY 300

Query: 314 GWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNG 373
                                      GF+  IM CCG GGPPLNYD ++ CG T   NG
Sbjct: 301 ---------------------------GFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNG 360

Query: 374 TLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSD 405
           T++TAK C DSS+Y+NWDGIHYTEAAN++V+  ILTGKYS+
Sbjct: 361 TIITAKPCYDSSKYVNWDGIHYTEAANRFVALHILTGKYSE 372

BLAST of CmoCh12G011060 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 5.6e-82
Identity = 164/387 (42.38%), Postives = 224/387 (57.88%), Query Frame = 0

Query: 21  LLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSGRYSD 80
           L LL+L   +  ++  + P +FNFGDSNSDTG +V      I  P G  FF   +GR SD
Sbjct: 21  LALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRSTGRLSD 80

Query: 81  GRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFGVQVN 140
           GRL++DFL  +++   L  YLDSLG   F+ G N+A AGS  LP       PFS  +QV 
Sbjct: 81  GRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV----PFSLNIQVK 140

Query: 141 QFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAF-YSKTLDQILA 200
           QF HFK++ LEL        L     +++ F+  LYM DIGQND+A +F    +  Q + 
Sbjct: 141 QFSHFKSRSLEL--ASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGNSYSQTVK 200

Query: 201 SIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSSHNQ 260
            IP I++E +S +++LYDEG R FWIHNTGPLGCL Q ++        LD+ GC+ S+N 
Sbjct: 201 LIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKSKDLDQHGCLVSYNS 260

Query: 261 AAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSVNPV 320
           AA LFN  L  +C++L+ +  D  + Y+DIY+IK +LIAN +Q                 
Sbjct: 261 AATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQY---------------- 320

Query: 321 TSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYIN 380
                      GF+ P+MACCGYGG P NY+ +I CGH     G+ V    C++ S +I+
Sbjct: 321 -----------GFKSPLMACCGYGGTPYNYNVKITCGH----KGSNV----CEEGSRFIS 364

Query: 381 WDGIHYTEAANQYVSSQILTGKYSDPP 407
           WDGIHYTE AN  V+ ++L+  YS PP
Sbjct: 381 WDGIHYTETANAIVAMKVLSMHYSKPP 364

BLAST of CmoCh12G011060 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 7.3e-82
Identity = 165/389 (42.42%), Postives = 229/389 (58.87%), Query Frame = 0

Query: 20  LLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSGRYS 79
           L+L   L V+     P   P +FNFGDSNSDTG +V     +I  P G  FF   +GR S
Sbjct: 20  LILRQNLAVAGGCQVP---PVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLS 79

Query: 80  DGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFGVQV 139
           DGRL++DFL  +++   LN YLDSL    F+ G N+A  GS+ LP     + PF+  +Q+
Sbjct: 80  DGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLP----RYVPFALNIQL 139

Query: 140 NQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTL--DQI 199
            QFLHFK++ LEL        L + +  +  F+  LYM DIGQND+A +F SK L   ++
Sbjct: 140 MQFLHFKSRALEL--ASISDPLKEMMIGESGFRNALYMIDIGQNDIADSF-SKGLSYSRV 199

Query: 200 LASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSSH 259
           +  IP ++SE +S ++ LYDEG R FW+HNTGPLGCL Q ++         D+ GC++++
Sbjct: 200 VKLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKLSM--VHSKGFDKHGCLATY 259

Query: 260 NQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSVN 319
           N AAKLFN  L  +C+ L+ +  + N+ YVDIY+IK +LIAN +                
Sbjct: 260 NAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNY-------------- 319

Query: 320 PVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEY 379
                        GFE+P+MACCGYGGPP NY+  I CG     NG    +K CD+ S +
Sbjct: 320 -------------GFEKPLMACCGYGGPPYNYNVNITCG-----NG---GSKSCDEGSRF 361

Query: 380 INWDGIHYTEAANQYVSSQILTGKYSDPP 407
           I+WDGIHYTE AN  V+ ++L+ ++S PP
Sbjct: 380 ISWDGIHYTETANAIVAMKVLSMQHSTPP 361

BLAST of CmoCh12G011060 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-77
Identity = 167/403 (41.44%), Postives = 226/403 (56.08%), Query Frame = 0

Query: 22  LLLLLCVSAAT--------SNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 81
           LLL+ CV  A+        S   + PA+FNFGDSNSDTG +  +  +  Y P G  FF  
Sbjct: 5   LLLVKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPY-PNGQTFFHS 64

Query: 82  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATAT----S 141
           PSGR+SDGRLI+DF+ + + +PYLNA+LDS+G+ NF  G N+A AGST+ P  AT     
Sbjct: 65  PSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGS-NFSHGANFATAGSTVRPPNATIAQSG 124

Query: 142 FCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDL-AGA 201
             P S  VQ+ QF  F  +  +L   +GG    K LP  +YF + LY FDIGQNDL AG 
Sbjct: 125 VSPISLDVQLVQFSDFITR-SQLIRNRGG-VFKKLLPKKEYFSQALYTFDIGQNDLTAGL 184

Query: 202 FYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKL 261
             + T DQI A IP +  +  + ++K+Y +G R FWIHNT PLGCL   + +F    S++
Sbjct: 185 KLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQI 244

Query: 262 DEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPC 321
           D  GC    N+ A+ +N +L     +L+ + S+   TYVDIYSIK  LI    +L     
Sbjct: 245 DNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKL----- 304

Query: 322 CTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNG-TLVT 381
                                 GF  P++ACCG+GG   N++  I CG   M+ G  +V 
Sbjct: 305 ----------------------GFRYPLVACCGHGG-KYNFNKLIKCGAKVMIKGKEIVL 364

Query: 382 AKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEK 411
           AK C+D S  ++WDGIH+TE  N ++  QI  G +SDPP   K
Sbjct: 365 AKSCNDVSFRVSWDGIHFTETTNSWIFQQINDGAFSDPPLPVK 375

BLAST of CmoCh12G011060 vs. ExPASy TrEMBL
Match: A0A6J1FBR9 (GDSL esterase/lipase At1g54790-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443981 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.3e-251
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 0

Query: 1   MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE 60
           MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE
Sbjct: 1   MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE 60

Query: 61  NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS 120
           NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS
Sbjct: 61  NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS 120

Query: 121 TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI 180
           TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI
Sbjct: 121 TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI 180

Query: 181 GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK 240
           GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK
Sbjct: 181 GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK 240

Query: 241 FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY 300
           FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY
Sbjct: 241 FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY 300

Query: 301 SQLGWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKM 360
           SQLGWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKM
Sbjct: 301 SQLGWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKM 360

Query: 361 LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF
Sbjct: 361 LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 419

BLAST of CmoCh12G011060 vs. ExPASy TrEMBL
Match: A0A6J1HPP3 (GDSL esterase/lipase At1g54790-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465565 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 1.9e-237
Identity = 398/406 (98.03%), Postives = 400/406 (98.52%), Query Frame = 0

Query: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73
           MA LTH  LLLLLCVSAATSNPFS PAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH
Sbjct: 1   MAPLTH--LLLLLCVSAATSNPFSRPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 60

Query: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133
           PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF
Sbjct: 61  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 120

Query: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193
           SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253
           LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 254 VSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLAS 313
           VSSHNQAAKLFNLQLHALCKKLQGQY+DGNVTYVDIYSIKSNLIANYS+LGWP CCTLAS
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYADGNVTYVDIYSIKSNLIANYSRLGWPSCCTLAS 300

Query: 314 FSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD 373
           FSVNPV SDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD
Sbjct: 301 FSVNPVKSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD 360

Query: 374 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF
Sbjct: 361 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 404

BLAST of CmoCh12G011060 vs. ExPASy TrEMBL
Match: A0A6J1FGI3 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443981 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 9.2e-229
Identity = 392/419 (93.56%), Postives = 392/419 (93.56%), Query Frame = 0

Query: 1   MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE 60
           MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE
Sbjct: 1   MPHSNSTPLPPPSMAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIE 60

Query: 61  NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS 120
           NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS
Sbjct: 61  NIYPPYGHRFFGHPSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGS 120

Query: 121 TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI 180
           TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI
Sbjct: 121 TILPATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDI 180

Query: 181 GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK 240
           GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK
Sbjct: 181 GQNDLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAK 240

Query: 241 FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY 300
           FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY
Sbjct: 241 FGTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANY 300

Query: 301 SQLGWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKM 360
           SQL                           GFEQPIMACCGYGGPPLNYDSRIVCGHTKM
Sbjct: 301 SQL---------------------------GFEQPIMACCGYGGPPLNYDSRIVCGHTKM 360

Query: 361 LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF
Sbjct: 361 LNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 392

BLAST of CmoCh12G011060 vs. ExPASy TrEMBL
Match: A0A6J1HML2 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465565 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.1e-216
Identity = 373/406 (91.87%), Postives = 375/406 (92.36%), Query Frame = 0

Query: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73
           MA LTH  LLLLLCVSAATSNPFS PAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH
Sbjct: 1   MAPLTH--LLLLLCVSAATSNPFSRPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 60

Query: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133
           PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF
Sbjct: 61  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 120

Query: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193
           SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253
           LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 254 VSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLAS 313
           VSSHNQAAKLFNLQLHALCKKLQGQY+DGNVTYVDIYSIKSNLIANYS+L          
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYADGNVTYVDIYSIKSNLIANYSRL---------- 300

Query: 314 FSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD 373
                            GFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD
Sbjct: 301 -----------------GFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD 360

Query: 374 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF
Sbjct: 361 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 377

BLAST of CmoCh12G011060 vs. ExPASy TrEMBL
Match: A0A1S3C007 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495321 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 7.9e-204
Identity = 344/406 (84.73%), Postives = 363/406 (89.41%), Query Frame = 0

Query: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73
           M+ LTH+L   LLC S A SNPFS PAVFNFGDSNSDTGC+VGAAIE+I PPYGHRFFGH
Sbjct: 1   MSTLTHILTFFLLCFSFARSNPFSRPAVFNFGDSNSDTGCLVGAAIESINPPYGHRFFGH 60

Query: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133
           PSGRY DGRL+VDFLLDAMDMP+LNAYLDS+GAPNFRKGCNYAAAGST+LPATATS  PF
Sbjct: 61  PSGRYCDGRLVVDFLLDAMDMPFLNAYLDSIGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193
           SFGVQVNQFLHFKA+VLELR GKGGKKLDKYLPA+DYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253
           LDQILASIP IL+EFESGVQ+LYD+GARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQRLYDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 254 VSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLAS 313
           VSSHNQAAKLFNLQLHALCKKLQGQY+DGN+TY+DIYSIKSNLIANYS+L          
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNITYIDIYSIKSNLIANYSRL---------- 300

Query: 314 FSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDD 373
                            GF+QPIM CCGYGGPPLNYDSRIVCG TKMLNGTLVTAKGCDD
Sbjct: 301 -----------------GFQQPIMVCCGYGGPPLNYDSRIVCGQTKMLNGTLVTAKGCDD 360

Query: 374 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFS+KMPFLLKLKF
Sbjct: 361 SSEYINWDGIHYTEAANQYVSSQILTGKYSDPPFSDKMPFLLKLKF 379

BLAST of CmoCh12G011060 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 560.5 bits (1443), Expect = 1.2e-159
Identity = 267/400 (66.75%), Postives = 313/400 (78.25%), Query Frame = 0

Query: 20  LLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSGRYS 79
           L +L+ L + ++ S     PA+ NFGDSNSDTG ++ A IEN+ PPYG  +F  PSGRY 
Sbjct: 12  LFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYC 71

Query: 80  DGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFGVQV 139
           DGRLIVDFLLD MD+P+LN YLDSLG PNF+KGCN+AAAGSTILPA  TS  PFSF +Q+
Sbjct: 72  DGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQI 131

Query: 140 NQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTLDQILA 199
           +QF+ FK++ +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ+LA
Sbjct: 132 SQFIRFKSRAIEL-LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLA 191

Query: 200 SIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSSHNQ 259
           SIP+IL  FE+G+++LY+EG RN WIHNTGPLGCLAQN+AKFGTD +KLDEFGCVSSHNQ
Sbjct: 192 SIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQ 251

Query: 260 AAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSVNPV 319
           AAKLFNLQLHA+  K Q QY D NVTYVDI+SIKSNLIANYS+                 
Sbjct: 252 AAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRF---------------- 311

Query: 320 TSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYIN 379
                      GFE+P+MACCG GG PLNYDSRI CG TK+L+G  VTAK C+DSSEYIN
Sbjct: 312 -----------GFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYIN 371

Query: 380 WDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           WDGIHYTEAAN++VSSQILTGKYSDPPFS++MPF L LKF
Sbjct: 372 WDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 383

BLAST of CmoCh12G011060 vs. TAIR 10
Match: AT1G54790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 541.2 bits (1393), Expect = 7.4e-154
Identity = 260/404 (64.36%), Postives = 316/404 (78.22%), Query Frame = 0

Query: 17  LTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSG 76
           L +++L  +  +  + S  F+ P+ FNFGDSNSDTG +V      +  P G   F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 77  RYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFG 136
           R+ DGRL++DFL+D MD+P+LN YLDSLG PNF+KGCN+AAAGSTILPA  TS  PFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 137 VQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 196
           +Q++QF+ FK++ +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIEL-LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 197 ILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSS 256
           +LASIP+IL  FE+G+++LY+EG RN WIHNTGPLGCLAQN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 257 HNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFS- 316
           HNQAAKLFNLQLHA+  K Q QY D NVTYVDI+SIKSNLIANYS+ G      L   + 
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNH 307

Query: 317 VNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSS 376
           +  V  + +L  ++ GFE+P+MACCG GG PLNYDSRI CG TK+L+G  VTAK C+DSS
Sbjct: 308 LENVGYNKIL--NVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSS 367

Query: 377 EYINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           EYINWDGIHYTEAAN++VSSQILTGKYSDPPFS++MPF L LKF
Sbjct: 368 EYINWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 408

BLAST of CmoCh12G011060 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.2e-151
Identity = 256/403 (63.52%), Postives = 306/403 (75.93%), Query Frame = 0

Query: 17  LTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSG 76
           L +++L  +  +  + S  F+ P+ FNFGDSNSDTG +V      +  P G   F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 77  RYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFG 136
           R+ DGRL++DFL+D MD+P+LN YLDSLG PNF+KGCN+AAAGSTILPA  TS  PFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 137 VQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 196
           +Q++QF+ FK++ +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIEL-LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 197 ILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSS 256
           +LASIP+IL  FE+G+++LY+EG RN WIHNTGPLGCLAQN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 257 HNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSV 316
           HNQAAKLFNLQLHA+  K Q QY D NVTYVDI+SIKSNLIANYS+              
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRF------------- 307

Query: 317 NPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSE 376
                         GFE+P+MACCG GG PLNYDSRI CG TK+L+G  VTAK C+DSSE
Sbjct: 308 --------------GFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSE 367

Query: 377 YINWDGIHYTEAANQYVSSQILTGKYSDPPFSEKMPFLLKLKF 420
           YINWDGIHYTEAAN++VSSQILTGKYSDPPFS++MPF L LKF
Sbjct: 368 YINWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 382

BLAST of CmoCh12G011060 vs. TAIR 10
Match: AT3G05180.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 3.0e-123
Identity = 225/401 (56.11%), Postives = 278/401 (69.33%), Query Frame = 0

Query: 14  MAALTHLLLLLLLCVSAA-TSNPFSS--------PAVFNFGDSNSDTGCVVGAAIENIYP 73
           M  L H LL LLL V+ + T +P +         PAVFNFGDSNSDTG +         P
Sbjct: 1   METLFHTLLRLLLFVAISHTLSPLAGSFRISNDFPAVFNFGDSNSDTGELSSGLGFLPQP 60

Query: 74  PYGHRFFGHP-SGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTIL 133
            Y   FF  P SGR+ +GRLIVDFL++A+D PYL  YLDS+    +R+GCN+AAA STI 
Sbjct: 61  SYEITFFRSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAASTIQ 120

Query: 134 PATATSFCPFSFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQN 193
            A A S+ PF FGVQV+QF+ FK+KVL+L   +  ++L +YLP++ +F  GLYMFDIGQN
Sbjct: 121 KANAASYSPFGFGVQVSQFITFKSKVLQLI--QQDEELQRYLPSEYFFSNGLYMFDIGQN 180

Query: 194 DLAGAFYSKTLDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGT 253
           D+AGAFY+KT+DQ+LA +P IL  F+ G+++LY EGARN+WIHNTGPLGCLAQ V+ FG 
Sbjct: 181 DIAGAFYTKTVDQVLALVPIILDIFQDGIKRLYAEGARNYWIHNTGPLGCLAQVVSIFGE 240

Query: 254 DPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQL 313
           D SKLDEFGCVS HNQAAKLFNLQLH L KKL  QY +   TYVDI+SIKS+LI N+S+ 
Sbjct: 241 DKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIFSIKSDLILNHSKY 300

Query: 314 GWPPCCTLASFSVNPVTSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNG 373
                                      GF+  IM CCG GGPPLNYD ++ CG T   NG
Sbjct: 301 ---------------------------GFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNG 360

Query: 374 TLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYSD 405
           T++TAK C DSS+Y+NWDGIHYTEAAN++V+  ILTGKYS+
Sbjct: 361 TIITAKPCYDSSKYVNWDGIHYTEAANRFVALHILTGKYSE 372

BLAST of CmoCh12G011060 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 4.0e-83
Identity = 164/387 (42.38%), Postives = 224/387 (57.88%), Query Frame = 0

Query: 21  LLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGHPSGRYSD 80
           L LL+L   +  ++  + P +FNFGDSNSDTG +V      I  P G  FF   +GR SD
Sbjct: 21  LALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRSTGRLSD 80

Query: 81  GRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPFSFGVQVN 140
           GRL++DFL  +++   L  YLDSLG   F+ G N+A AGS  LP       PFS  +QV 
Sbjct: 81  GRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV----PFSLNIQVK 140

Query: 141 QFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAF-YSKTLDQILA 200
           QF HFK++ LEL        L     +++ F+  LYM DIGQND+A +F    +  Q + 
Sbjct: 141 QFSHFKSRSLEL--ASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGNSYSQTVK 200

Query: 201 SIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVSSHNQ 260
            IP I++E +S +++LYDEG R FWIHNTGPLGCL Q ++        LD+ GC+ S+N 
Sbjct: 201 LIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKSKDLDQHGCLVSYNS 260

Query: 261 AAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGWPPCCTLASFSVNPV 320
           AA LFN  L  +C++L+ +  D  + Y+DIY+IK +LIAN +Q                 
Sbjct: 261 AATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQY---------------- 320

Query: 321 TSDDLLHFDMAGFEQPIMACCGYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYIN 380
                      GF+ P+MACCGYGG P NY+ +I CGH     G+ V    C++ S +I+
Sbjct: 321 -----------GFKSPLMACCGYGGTPYNYNVKITCGH----KGSNV----CEEGSRFIS 364

Query: 381 WDGIHYTEAANQYVSSQILTGKYSDPP 407
           WDGIHYTE AN  V+ ++L+  YS PP
Sbjct: 381 WDGIHYTETANAIVAMKVLSMHYSKPP 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ECP61.0e-15264.36GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2... [more]
Q9MAA14.3e-12256.11GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2... [more]
Q9FXB65.6e-8242.38GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1[more]
O805227.3e-8242.42GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2... [more]
Q9LIN22.4e-7741.44GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1FBR91.3e-251100.00GDSL esterase/lipase At1g54790-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HPP31.9e-23798.03GDSL esterase/lipase At1g54790-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1FGI39.2e-22993.56GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HML23.1e-21691.87GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3C0077.9e-20484.73GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
AT1G54790.31.2e-15966.75GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.27.4e-15464.36GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.11.2e-15163.52GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G05180.13.0e-12356.11GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G56670.14.0e-8342.38GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 41..397
e-value: 7.9E-47
score: 160.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 29..400
e-value: 1.5E-60
score: 207.3
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 22..419
NoneNo IPR availablePANTHERPTHR22835:SF536OS05G0401000 PROTEINcoord: 22..419
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 39..400
e-value: 8.48989E-110
score: 323.798

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G011060.1CmoCh12G011060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds