CmoCh12G010210 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G010210
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	PMEI domain-containing protein
Location	Cmo_Chr12: 9540922 .. 9542838 (-)
RNA-Seq Expression	CmoCh12G010210
Synteny	CmoCh12G010210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTCTCGGCGTGTTCTTCGCAACGGCTAGCCTTGTTCTTCGTGTTCTTCGTGTTCTTCATTGTTCATCCTGTCTTGGGTGACCCTGCAACGCAGCAACGGATCGACAGAGTATGTCGTCAAATGGAGGATTATGGATTTTGCAATAGAACCTTTAGTGAAAATCTGAGAGGTCCAGTAGATGATGTTGGCTTAACCTTGATAGCAAATGACCAAGTTTTGAGAAATGCGTCCAATACCTATGAGTTCATAGAGCAGCTTCAAGCTAGGACATCCGATCCAGCAACAAGAGCTGCACTTGAGAATTGCAGAATTGGTTGCAGCCAGGTCCGGGAAGCGTTTGAGCAGGCGAGTTATTACTTCAACAAAAGAGACTATGCCAGTATGGTCGAGGTTGAAAGGGATGCACCCAGAGGAGAGGCATTCTGTCGACCGCCGACGACGGCGAGCCGGTTGGTTGAGAGGAAGAGGGAATTGAGGATTCTCATTGCCATGGCTGTGGTTGCTGGTCATATACTAGCTCCTTGATCGACTCGGTTGTTGTTATGTATCGTAGTCAGCCTCATGGTTTTAAAACGCGTCTACTAGAGAGAGGTTTCCACACCCTAAGAAATGCTTCGTTCTCCTCTCCAATCGATGTAGGACCTGATGCTATCTCCTTTTATAGGTTGATAATAGAGAAAGGATTAATCCTACCGATCAAAAGTCGTTCAAAAGTCGTTCATGTGGCCTCTAAACAGTCTTTAACTCTACTTCCTTTCTGGCGCGACAAAATGCAATGACAAATGTTTAACATAAACAGCTACAAAGATGAGGTTTAGAAATCTAATTTTGCAGTTCCTGTTGTATCATATACTATTCTTTTCACCAAAAAATTTGGTAGAGGCGATTATAATATAACAAAAAAAGAAAGCTGCATTTTGGCGACTGAAGTTCAATAGCATTTTCGTCAATCTAGATGTAACGGTCCAAACCCACCGCTAGTAGATATTGTCTCTTTGAGCTTTCCGTTTTGGGATTTCCCTTAATGTTTTTAAAACGCAGGTGGGGAGAGGTTTCCACATCCTTATAAAGAATGTTTCATTCTCCTCCCCAACCGTTGTGGGATCTCACAATCCACCCTCTTTGAGGCTCAGCCTCCTCGCTAGCACACTGCCTTGTGTCCATCTCCCCCTACGGGGCTCAGCCACCTCGCTGGCACATCATTAGCGGTGGGCTTGGACCGTTACAAATGATATCAGAGCTAGACACCGAGCAATATGTCAGCGAGGAGGCTAAACCCTGAAAGGGGTGGACATGAGGCGGTGTGCTAGTGATGTGCTAGCGAGGAGGTTGAGCCCTGAAGGGGTGGACATGATGTGGTGTGTCAGCGATGTGCCAGCGAGGAGGCTGAGCCCCGAAGGGGGGTGGACACGAAGTGGTGTGCCAGCGAGTAGACTGAGCCTCGAAGAGGGGTGGACACAAGGCAGTGTGCCAACGAGGAGGCTGGGCCACGAAGGAGAGTGGATTGTGAGATCCCACATTGGTTGGGGAGAAGAAGGAAACATCCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACGCATTTTAAAACTTTGAGAGGAATCCCAAAAGGGAAAGCAAACTTGAGGGGAATTACAAAAGAGAAAATCTAAAAAAGACAATATCAGCTGGCGGTAGGCTCAACTAATTACGACATTATAATCGCAACAAAAAAATCATTAGAGATTTGAATCCTCATATTTTTGTTAAACTGAAAACAAAGAAAAAAAAGAAATCAACTAGAACTTCAATTGTGGTTTCCCATTTTGGAACTTAATTGATAATCTTAAGATCACAGTTTCTGAGACAGTATCCATGTGTTTGATGGAAACAGAGTTTACAAAGAACAAGAAGGAGAAACGTACCAGTAA

mRNA sequence

ATGGCGTTCTCGGCGTGTTCTTCGCAACGGCTAGCCTTGTTCTTCGTGTTCTTCGTGTTCTTCATTGTTCATCCTGTCTTGGGTGACCCTGCAACGCAGCAACGGATCGACAGAGTATGTCGTCAAATGGAGGATTATGGATTTTGCAATAGAACCTTTAGTGAAAATCTGAGAGGTCCAGTAGATGATGTTGGCTTAACCTTGATAGCAAATGACCAAGTTTTGAGAAATGCGTCCAATACCTATGAGTTCATAGAGCAGCTTCAAGCTAGGACATCCGATCCAGCAACAAGAGCTGCACTTGAGAATTGCAGAATTGGTTGCAGCCAGGTCCGGGAAGCGTTTGAGCAGGCGAGTTATTACTTCAACAAAAGAGACTATGCCAGTATGGTCGAGGTTGAAAGGGATGCACCCAGAGGAGAGGCATTCTGTCGACCGCCGACGACGGCGAGCCGTATCCATGTGTTTGATGGAAACAGAGTTTACAAAGAACAAGAAGGAGAAACGTACCAGTAA

Coding sequence (CDS)

ATGGCGTTCTCGGCGTGTTCTTCGCAACGGCTAGCCTTGTTCTTCGTGTTCTTCGTGTTCTTCATTGTTCATCCTGTCTTGGGTGACCCTGCAACGCAGCAACGGATCGACAGAGTATGTCGTCAAATGGAGGATTATGGATTTTGCAATAGAACCTTTAGTGAAAATCTGAGAGGTCCAGTAGATGATGTTGGCTTAACCTTGATAGCAAATGACCAAGTTTTGAGAAATGCGTCCAATACCTATGAGTTCATAGAGCAGCTTCAAGCTAGGACATCCGATCCAGCAACAAGAGCTGCACTTGAGAATTGCAGAATTGGTTGCAGCCAGGTCCGGGAAGCGTTTGAGCAGGCGAGTTATTACTTCAACAAAAGAGACTATGCCAGTATGGTCGAGGTTGAAAGGGATGCACCCAGAGGAGAGGCATTCTGTCGACCGCCGACGACGGCGAGCCGTATCCATGTGTTTGATGGAAACAGAGTTTACAAAGAACAAGAAGGAGAAACGTACCAGTAA

Protein sequence

MAFSACSSQRLALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGPVDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASYYFNKRDYASMVEVERDAPRGEAFCRPPTTASRIHVFDGNRVYKEQEGETYQ

Homology

[Close]

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL
Match: A0A6J1DQ57 (uncharacterized protein LOC111023296 OS=Momordica charantia OX=3673 GN=LOC111023296 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.5e-55
Identity = 110/151 (72.85%), Postives = 125/151 (82.78%), Query Frame = 0


Query: 1   MAFSACSSQRLALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGP 60
           MA S CS+Q LALF    V  +V P   DPATQQRIDR+CRQMEDYGFCNRTF+ENLRGP
Sbjct: 1   MALSECSTQLLALFLTLVV--VVRPASSDPATQQRIDRICRQMEDYGFCNRTFNENLRGP 60

Query: 61  VDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASY 120
            DDVGLTLIAN+Q LRNASNTY+F+ QLQA   D ATRAALENCRIGC+ V++AF QAS+
Sbjct: 61  ADDVGLTLIANNQALRNASNTYQFVVQLQASEVDLATRAALENCRIGCNVVKQAFLQASF 120

Query: 121 YFNKRDYASMVEVERDAPRGEAFCRPPTTAS 152
           +FN+RDYA+MVEVE+ APRGEA CRPPT  S
Sbjct: 121 HFNQRDYANMVEVEKAAPRGEALCRPPTPDS 149

[Close]

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL
Match: A0A1S3BWX8 (uncharacterized protein LOC103494023 OS=Cucumis melo OX=3656 GN=LOC103494023 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 9.5e-31
Identity = 78/151 (51.66%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 1   MAFSACSSQRLALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGP 60
           MAFS  S     L  +F +  +VHP LG+P TQQ IDR+C Q EDYGFCN+T +ENL+GP
Sbjct: 1   MAFSYSSQ----LQTLFLILLVVHPALGNPETQQWIDRICYQNEDYGFCNKTSNENLKGP 60

Query: 61  VDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASY 120
            DDVGL LIAN+QVLRN S TY+FI +LQA T DP T+ ALE CRI              
Sbjct: 61  ADDVGLVLIANNQVLRNTSRTYQFIVELQASTDDPVTKTALEACRI-------------- 117

Query: 121 YFNKRDYASMVEVERDAPRGEAFCRPPTTAS 152
                           APRGEA CRPPT  S
Sbjct: 121 ----------------APRGEASCRPPTPDS 117

[Close]

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL
Match: A0A6J1DRX6 (uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023283 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 4.4e-28
Identity = 70/144 (48.61%), Postives = 91/144 (63.19%), Query Frame = 0


Query: 1   MAFSACSSQRLALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGP 60
           MA S CS     L  +FF+F I+   LGDP T++RI+RVCRQME++GFC++TF ENL+GP
Sbjct: 1   MASSRCSLSSQLLLGLFFIFLIMGSTLGDPETEERINRVCRQMEEFGFCSQTFHENLKGP 60

Query: 61  VDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASY 120
            D V LT IA  + + NA+ T  +I +L A  SDPA +  L  C      + +AF +   
Sbjct: 61  TDYVRLTQIAKVEAIINATRTLRYITKLLAFVSDPALKNNLIVCENAYDIMNKAFLEGIK 120

Query: 121 YFNKRDYASMVEVERDAPRGEAFC 145
           YF +RDY SMV  ER APR EA C
Sbjct: 121 YFFQRDYRSMVNAERIAPRAEASC 144

[Close]

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL
Match: A0A6J1G4X2 (uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC111450843 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.7e-27
Identity = 66/144 (45.83%), Postives = 87/144 (60.42%), Query Frame = 0


Query: 1   MAFSACSSQRLALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGP 60
           M F+ C    L L  +  VFFIV  V+GD  T+ RID +C QME++GFC++TF ENL GP
Sbjct: 1   MVFTRCFPSSLMLLVLIVVFFIVQSVVGDSDTEDRIDNLCHQMEEFGFCSQTFHENLNGP 60

Query: 61  VDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASY 120
            DD+GLT IA  Q   NAS T  +I++L    +DPA +  L  C      V EA ++   
Sbjct: 61  TDDIGLTQIAIFQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEALQEGIE 120

Query: 121 YFNKRDYASMVEVERDAPRGEAFC 145
            F K+DY SM+ VE+ APR +A C
Sbjct: 121 LFFKKDYRSMLNVEKIAPRAQASC 144

[Close]

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL
Match: A0A4U5N9E9 (Invertase/pectin methylesterase inhibitor family protein OS=Populus alba OX=43335 GN=D5086_0000271680 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.5e-25
Identity = 69/147 (46.94%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 1   MAFSACSSQRLALFF-VFFVFFIVHPVLGDPA-TQQRIDRVCRQMEDYGFCNRTFSENLR 60
           MAFS C S    LFF +  +  + +P L +   +Q  ID VCRQMEDYGFCNR F EN++
Sbjct: 1   MAFSHCFS----LFFPLLVIILLTNPTLSEAQDSQVLIDEVCRQMEDYGFCNRVFHENMK 60

Query: 61  GP-VDDVGLTLIANDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQ 120
            P +D VGLT IA DQ + NA+NTYE+I  L   T+D + R  L  C    + V+ +F  
Sbjct: 61  SPSIDYVGLTAIAMDQTITNATNTYEYILDLLRNTTDQSLRNVLVACENAFAIVKSSFGD 120

Query: 121 ASYYFNKRDYASMVEVERDAPRGEAFC 145
           A   FN++DY  M ++ERDAPR +A C
Sbjct: 121 ALQSFNRKDYDDMFKLERDAPRAQASC 143

[Close]

BLAST of CmoCh12G010210 vs. TAIR 10
Match: AT1G09360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 2.7e-09
Identity = 35/122 (28.69%), Postives = 59/122 (48.36%), Query Frame = 0


Query: 16  VFFVFFIVHP----VLGDPATQQRIDRVCRQMEDYGFCNRTFSENLRGPVDDV-GLTLIA 75
           +  V +I  P    V  D  T+  I+ +C + EDYGFCN+   E L+ P   +  LT + 
Sbjct: 2   IVLVLYIFTPHIEGVEADGPTEALINSICIENEDYGFCNKIIHEKLKTPTATIKELTSLI 61

Query: 76  NDQVLRNASNTYEFIEQLQARTSDPATRAALENCRIGCSQVREAFEQASYYFNKRDYASM 133
               + +AS+TY FI+ +  +   P   + L+ C     +   +F +  + F+K +Y  M
Sbjct: 62  IYTTMAHASDTYIFIDNILRKWPGPKETSGLKTCHAVYKRETNSFLEIRFLFSKGEYELM 121

[Close]

BLAST of CmoCh12G010210 vs. TAIR 10
Match: AT1G09370.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 7.7e-09
Identity = 34/118 (28.81%), Postives = 55/118 (46.61%), Query Frame = 0


Query: 29  DPATQQRIDRVCRQMEDYGFCNRTFSENLRG-PVDDVGLTLIANDQVLRNASNTYEFIEQ 88
           D  TQQR++ +C+Q  D  FC+    +NL   P  +  +  +   +  R A+NTY FI  
Sbjct: 23  DEKTQQRVNGICKQTMDTRFCSSLLIKNLNTFPASNKEIMNVTVSEAERFAANTYFFIST 82

Query: 89  LQARTSDPATRAALENCRIGCSQVREAFEQASYYFNKRDYASMVEVERDAPRGEAFCR 146
           L     D   R  L+ C    + V  AF +A  +F +  Y+ +V +E+        C+
Sbjct: 83  LLRNAGD--ERPDLQACAEAYAIVNSAFTKAVTFFKQAYYSKIVNIEKKVSMAVDICK 138

[Close]

BLAST of CmoCh12G010210 vs. TAIR 10
Match: AT3G49330.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 9.5e-07
Identity = 39/137 (28.47%), Postives = 57/137 (41.61%), Query Frame = 0


Query: 11  LALFFVFFVFFIVHPVLGDPATQQRIDRVCRQMEDYGFCNRTFSENL-RGPVDDVGLTLI 70
           L    +F  F     V+ DP TQ  I+R+C+Q  D+ FCN+T +  L R       +  +
Sbjct: 7   LMSLLIFITFKSSISVVPDPPTQNLINRICKQTIDFKFCNQTITSQLVRRQTSIKTIAKL 66

Query: 71  ANDQVLRNASNTYEFIE-QLQARTSDPATRAALENCRIGCSQVREAFEQASYYFNKRDYA 130
              +   NA  T + IE  L  +  D   +A  + CR     V    + A  Y   RDY 
Sbjct: 67  TAAKAWINAIQTLDNIEDSLLPKAKDKRDKAEFDACRKAYKLVDAHLDNALKYLYLRDYR 126

Query: 131 SMVEVERDAPRGEAFCR 146
            M   +  A    + CR
Sbjct: 127 FMRAYQALALVNISMCR 143

The following BLAST results are available for this feature:

BLAST of CmoCh12G010210 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh12G010210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DQ57	1.5e-55	72.85	uncharacterized protein LOC111023296 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A1S3BWX8	9.5e-31	51.66	uncharacterized protein LOC103494023 OS=Cucumis melo OX=3656 GN=LOC103494023 PE=...	[more]
A0A6J1DRX6	4.4e-28	48.61	uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1G4X2	1.7e-27	45.83	uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC1114508...	[more]
A0A4U5N9E9	3.5e-25	46.94	Invertase/pectin methylesterase inhibitor family protein OS=Populus alba OX=4333...	[more]

BLAST of CmoCh12G010210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09360.1	2.7e-09	28.69	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G09370.1	7.7e-09	28.81	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G49330.1	9.5e-07	28.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..162 e-value: 2.6E-14 score: 55.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..145
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..123 e-value: 1.5E-9 score: 38.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 11..144 e-value: 3.9E-20 score: 69.9
None	No IPR available	PANTHER	PTHR36710:SF1	F14J9.2 PROTEIN	coord: 9..160
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 9..160
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 32..164 e-value: 2.15279E-23 score: 88.2481

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G010210.1	CmoCh12G010210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity