CmoCh12G008810 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G008810
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMechanosensitive ion channel protein 1
LocationCmo_Chr12: 8017112 .. 8021272 (-)
RNA-Seq ExpressionCmoCh12G008810
SyntenyCmoCh12G008810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCTTTTCATTCTCAATTCGATTTATAGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACTGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCCTAAGAGCACCCTCTGTATGTTGTCTACACACACCAAGTTATGGTACACATCCCGCTGCTACTTTTTCCCCCGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACGTCTCTTGGCTATCTGTCAAACCCTCGATTCTATTCATCAACTTCTAGCAGGAAGGCGGATATTACTGGAGATACTGGTGTTCCACTTGCTTCTTCTGGTGTTGAAGCGGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGTTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGCTCTTAGATTCACATCCATATCTGAAAAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATGTTAGTGACTCAAACTTCAACATATTTCTCTTTAATTACACAATGGTTTGTTGACTGTGTGCTTGATCTTTGCTTGAAACATTTGTCTAAACTTTGATTTGCAGTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTATGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGTGAGAGCTTGCTGCCAAATGGCTTTGCAGTTTTACTTTCGTTTGACTGATTCCTACTTTGCTGTCATGTGTCCTCCACTTGATTGGTTTATTGGTAGGGCCGAAGGTTTAACATTGTTCATCATTCTTTTTTCAAATTAAAATAAAAAAAGAGAGAAAACTCTTCTCTTGATTATTGAACCTTACCAATGTAGCTCTTTTTGTATTGTTGTGGAGTAGAGTTATGCTCAAGATAACAATCATGTTCTTCGTAATGATATGCTTCGATTTTTAGAGCGGTAATCAGGCTTGCATATTTTTAATAACTCGAAATATGTATAGCATGATTTAAATCTCCGATTTCTTTGCAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGAAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGTGTGTAAATCATACTTTTTTCTCTTTTATCTTTGGGCAAGGAACCAAGTTGTATCTAGCATTGCTGGGGCACATATGATCCACAAAAGTTGGAAGATTTGAAGTGGGACTTCATTTTTTTTTTTTTTTTTCCTTTGACTACTATCATTCTTATTCTCCTAATAAAAACGTCACTGAAGTTGATACGTCAATGATGCTTGGTCTTTTAGATGACATTCATCTTTGTATACTATCAAATCCATGTGTACTTTTCAGTCATTAACCTCTCTTTGCATTTACTGGGAGATGATGGCATTGAACAAATTGCTGGAAACTTATAGATTGTTTCTGCAAATTTGTTCTATTTGTTCTTTCTCATCAACTTCAAAAACGAATGATTTGCAATTCCATTTGCAGGCTGGTGCTATTGAAGGCCAAGTGGTGGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTGAATCTTCTTTTTTTTCTTTTTTGCTTTTTTGTGCTTGGATTTTTTATTTATTTACTATATCATCATTATCTTCATGGTCGTTTTATTTTTGTTCCTGAACGACGCTTCCCTTATTTAAATTGTTTACTAAAAGTCGAGGCATATTTGACTATTAGTTTCCATAGCCGAACGATGCATATGTTGCCAATAACTTGATTAAGTACATTTGTTGTCATGAAGATGTGTTGCCAATAATATGATTAAATACATTTGTTATCATGGAGATTTAAGTGATATTGGCTTTCTATTTGAAATTAGTGTAGAAGATAGAGTAAATTTGTTGTCCTCTCGATGCCTTTCCTCATGAAGAGCATGTGAATACGTCCAACTTCATTTTTCTGGTAGTCTCGCAAGTAGATTTCTAAATTAAGTTAATTATCCATTTTGATATCTTTATAAATTTTATTAAAAAATATACCATTTTTAGCTCCCGTATGGAGAAAAATCGACCAATATTCAACTTTTCGGACTTGGAGATGGTCAGTAGTCACGACACGAATGGCGATTGTTCTTATCTTTCACTCGGCAAACTTTCCTGTAATGCTAGATCATTTAATCTATGTTTGATTCTTTAATTCATGCAGGGATATGTTTGGCATATGATTTGTTGTGTTTTAATGATTTCGCATGAATCTCTTAGTTGATTATGTAATGAAGGTATTTGTTCATTTGGTCCCACATTTTTCAACCCGTTTCTTGAATTTATGCTGAGGATTGCGATGGATTTCTTGGATTAAGATGGTGATTTTGATCAATTTTACTGCGTGGTTAAATGGGATTATAATATTTACAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATCTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGTATTCTTGGGCAAGGAGGCTCCTTATTGCTTCCTCTCTCAAATTGAAAGTTCGTATGCAGAGCTGACAATTGGTTGCAACCTTAAAAGGATGGTGAGTCAGTCTAATATTTTGAGTGTCATATGCTAGGAATCAATACTCCTCTCTACGATGGTATGATATTGTCCATTTTGAGCAAGCTCTCGTGGCTTTTCTTTGGGCTTCCCCAAAATCCCTCGTACCAATGGATATGTATTCCTTACTTATAAATCCATGATCATTCTTTTAATTAACCAACGTGGGACTCCCTCCCAACAATCCTCAACAATCCTCCTCTCGAACAAAGTACACCATAGAGCCTCCTGTAAGGCCTATGGAGCCCTTGCACAACCTCCCCTTAATTGAGGCTCAACTCCTTTCTCTGGAGTCCTCAAACAAAGTATACCTTTTGTTCGACACTTGAGTCACTTTGACTACACTCTCGTGGGTCTCAACTTCTTTGTTCGACATTTGAGGATTCTTTTGACATGGCGAAGTTAAGGGCATGGCTCTGATACCATGTTAGAAATCGTAGATCTCCACAATGGTATGATGATAATGTCCACTTTGAGCATAAGCTCTCATGGCTTTGCATACCAATGGTGATGTATTCCTTACTTATAAACCCATGATCTTTCCTTTAATTAGCTAACGTGGGTCTCCCTCCCAACAATCCTCAACATCATATCCTCCTCGAACTCCTCGACTTGTTGTCCCTTCAAACGTTAGAGTTTCATACTTCAAAATTGTATTTATGTAGGGTAAAGAAGAACTTTTCTCAACAGAGCAAGATATTCTCCTCCAGTCAGTACAGATCATGAAGGAAAATGGTGCAACCTTGGGTAACTGACGAACAAGATGCCAAACGTACTTTCAGGTAAGTCTCGTACTAATGGAGATTGATCTTAAGCTATTCCCATGCCACGATGTGACTGGACAGCCATAAATTTTCAAGGATGTTTTTTTCGAGGGATATTATTGAAATGAGTACACAATTTAAGGGTGTTTCTACAATTTTCTCGAAATTATTTTGCCTTAC

mRNA sequence

ATGAGGCTTTTCATTCTCAATTCGATTTATAGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACTGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCCTAAGAGCACCCTCTGTATGTTGTCTACACACACCAAGTTATGGTACACATCCCGCTGCTACTTTTTCCCCCGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACGTCTCTTGGCTATCTGTCAAACCCTCGATTCTATTCATCAACTTCTAGCAGGAAGGCGGATATTACTGGAGATACTGGTGTTCCACTTGCTTCTTCTGGTGTTGAAGCGGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGTTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGCTCTTAGATTCACATCCATATCTGAAAAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTATGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGAAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGCCAAGTGGTGGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATCTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGGTAAAGAAGAACTTTTCTCAACAGAGCAAGATATTCTCCTCCAGTCAGTACAGATCATGAAGGAAAATGGTGCAACCTTGGGTAACTGACGAACAAGATGCCAAACGTACTTTCAGGTAAGTCTCGTACTAATGGAGATTGATCTTAAGCTATTCCCATGCCACGATGTGACTGGACAGCCATAAATTTTCAAGGATGTTTTTTTCGAGGGATATTATTGAAATGAGTACACAATTTAAGGGTGTTTCTACAATTTTCTCGAAATTATTTTGCCTTAC

Coding sequence (CDS)

ATGAGGCTTTTCATTCTCAATTCGATTTATAGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACTGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCCTAAGAGCACCCTCTGTATGTTGTCTACACACACCAAGTTATGGTACACATCCCGCTGCTACTTTTTCCCCCGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACGTCTCTTGGCTATCTGTCAAACCCTCGATTCTATTCATCAACTTCTAGCAGGAAGGCGGATATTACTGGAGATACTGGTGTTCCACTTGCTTCTTCTGGTGTTGAAGCGGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGTTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGCTCTTAGATTCACATCCATATCTGAAAAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTATGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGAAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGCCAAGTGGTGGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATCTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGGTAAAGAAGAACTTTTCTCAACAGAGCAAGATATTCTCCTCCAGTCAGTACAGATCATGAAGGAAAATGGTGCAACCTTGGGTAACTGA

Protein sequence

MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNVSRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKGKEELFSTEQDILLQSVQIMKENGATLGN
Homology
BLAST of CmoCh12G008810 vs. ExPASy Swiss-Prot
Match: Q8VZL4 (Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MSL1 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.4e-134
Identity = 258/444 (58.11%), Postives = 328/444 (73.87%), Query Frame = 0

Query: 90  ASPVKYWNTIPPTS-LGYLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGR 149
           +S ++  N   P + LG+ S  R +SS    K+D  G     + +SGV     +    G 
Sbjct: 55  SSNIRLHNDAKPFNYLGHSSYARAFSS----KSDDFGS----IVASGVTGS-GDGNGNGN 114

Query: 150 DWLERVKDGWQSVVDAASFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAPVGMTLTATL 209
           DW+E+ KD  Q+ VDA + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL
Sbjct: 115 DWVEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTL 174

Query: 210 LAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQI 269
            AWVVMPR+LRRFH Y+M+S   +L    S E++PYEKSFWGALEDP RYLVTF AF+QI
Sbjct: 175 FAWVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQI 234

Query: 270 GKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDK 329
             MVAPT IA+++ S   +GAVILSLVWF+YRWKTNV +R L++++F GLDREK+LTLDK
Sbjct: 235 AAMVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDK 294

Query: 330 VSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFS 389
           VSSVGLFAIGLMA AEA GVAVQS+LTVGG+GGVATAFAARDILGNVLSGL+MQFS+PFS
Sbjct: 295 VSSVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFS 354

Query: 390 LGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIP 449
           +GDTIKAG++EGQV+EMGL TTSLL+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIP
Sbjct: 355 MGDTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIP 414

Query: 450 FQVADLSTVSKISDDIKSMLRSHPK-------------------------------GKEE 502
            Q+ DL  + +IS++IK MLRS+ K                               GKEE
Sbjct: 415 LQIDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEE 474

BLAST of CmoCh12G008810 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.8e-19
Identity = 63/186 (33.87%), Postives = 100/186 (53.76%), Query Frame = 0

Query: 289 AVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGV 348
           AVIL++  F+     N F +    Q   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFLL---INQFEK---QQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 349 AVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMG 408
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 409 LMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKS 468
              T + + +  P+ VPNSLFSS  + N  R   R IT  I  +  D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 469 MLRSHP 473
           ML++HP
Sbjct: 267 MLKNHP 266

BLAST of CmoCh12G008810 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.8e-19
Identity = 63/186 (33.87%), Postives = 100/186 (53.76%), Query Frame = 0

Query: 289 AVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGV 348
           AVIL++  F+     N F +    Q   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFLL---INQFEK---QQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 349 AVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMG 408
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 409 LMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKS 468
              T + + +  P+ VPNSLFSS  + N  R   R IT  I  +  D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 469 MLRSHP 473
           ML++HP
Sbjct: 267 MLKNHP 266

BLAST of CmoCh12G008810 vs. ExPASy Swiss-Prot
Match: O25170 (Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0415 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.3e-13
Identity = 58/195 (29.74%), Postives = 99/195 (50.77%), Query Frame = 0

Query: 288 GAV-ILSLVWFI---YRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALA 347
           GAV I+ L W +   ++         +A+++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNMATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 348 EASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVV 407
           +  G  V +++   GIGG+A A A +D+L N  + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 408 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQV-----ADLSTVS 467
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I  ++     +  S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 468 KISDDIKSMLRSHPK 474
               DIK ML +HPK
Sbjct: 503 LCVKDIKEMLENHPK 514

BLAST of CmoCh12G008810 vs. ExPASy Swiss-Prot
Match: Q9ZKG7 (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.0e-13
Identity = 58/195 (29.74%), Postives = 99/195 (50.77%), Query Frame = 0

Query: 288 GAV-ILSLVWFI---YRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALA 347
           GAV I+ L W +   ++         +A+++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNVATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 348 EASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVV 407
           +  G  V +++   GIGG+A A A +D+L N  + + +     FS GD I  G +EG VV
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 408 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQV-----ADLSTVS 467
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I  ++     +  S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 468 KISDDIKSMLRSHPK 474
               DIK ML +HPK
Sbjct: 503 LCVKDIKEMLENHPK 514

BLAST of CmoCh12G008810 vs. ExPASy TrEMBL
Match: A0A6J1FHY5 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444110 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.4e-276
Identity = 502/533 (94.18%), Postives = 502/533 (94.18%), Query Frame = 0

Query: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60
           MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60

Query: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120
           SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK
Sbjct: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120

Query: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180
           ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180

Query: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240
           PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE
Sbjct: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360
           WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 420
           GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI
Sbjct: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK------- 480
           VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK       
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 ------------------------GKEELFSTEQDILLQSVQIMKENGATLGN 503
                                   GKEELFSTEQDILLQSVQIMKENGATLGN
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIMKENGATLGN 533

BLAST of CmoCh12G008810 vs. ExPASy TrEMBL
Match: A0A6J1I1Q4 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111468640 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.9e-271
Identity = 491/533 (92.12%), Postives = 496/533 (93.06%), Query Frame = 0

Query: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60
           MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLR SVVLNKQYYKDESHSLKNV
Sbjct: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNV 60

Query: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120
           SRTGF+RAPSVC LHTPSYGTHP ATFSPASPVKYWNTIPPTSL YLSNPRFYSSTSSRK
Sbjct: 61  SRTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRK 120

Query: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFS 180
           ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQS VDAASFTGQKAREVSDEFS
Sbjct: 121 ADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFS 180

Query: 181 PHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240
           PHVDK+LDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE
Sbjct: 181 PHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIE 240

Query: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300
           EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR
Sbjct: 241 EIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYR 300

Query: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360
           WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG
Sbjct: 301 WKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIG 360

Query: 361 GVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPI 420
           GVATAFA+RDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFPI
Sbjct: 361 GVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPI 420

Query: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK------- 480
           VVPNSLFSSQVIINKSRAQWRAITKKIPFQVAD STVSKISDDIKSMLRSHPK       
Sbjct: 421 VVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEA 480

Query: 481 ------------------------GKEELFSTEQDILLQSVQIMKENGATLGN 503
                                   GKEELFSTEQDILLQSVQI+KENGATLGN
Sbjct: 481 PYCFLSQIESSYAELTIGCNLKRMGKEELFSTEQDILLQSVQIIKENGATLGN 533

BLAST of CmoCh12G008810 vs. ExPASy TrEMBL
Match: A0A6J1D280 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111016329 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.2e-220
Identity = 414/535 (77.38%), Postives = 449/535 (83.93%), Query Frame = 0

Query: 1   MRLFILNSIYSASKSYLNPCSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNV 60
           +R+FIL SIY++S+SYLNP S Q  K YTKISEYV+R LR S VLNK+ + D S   KN 
Sbjct: 4   IRIFILKSIYTSSRSYLNPYSSQSYKPYTKISEYVDRGLRPSTVLNKKCHNDNSRRTKNT 63

Query: 61  SRTGFLRAPSVCCLHTPSYGTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRK 120
           S TGF RA SVC L+  S+G +P  TF PA  V+YW T+P TS G+LSNPR YSS SSRK
Sbjct: 64  SGTGFPRAASVCGLNISSHGAYPIDTFLPAWSVRYWRTVPLTSPGHLSNPRLYSSASSRK 123

Query: 121 ADITGDTGVPLASSGVEADISNPPDV--GRDWLERVKDGWQSVVDAASFTGQKAREVSDE 180
           ADITGDTGV  AS+GVEADISN  DV  G++WLERVKD WQS VDAASFT QKA+EVSDE
Sbjct: 124 ADITGDTGVSAASAGVEADISNTQDVGTGKEWLERVKDVWQSAVDAASFTEQKAKEVSDE 183

Query: 181 FSPHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTS 240
           F+PHVDKL DSHPYLKN I PV MTLTATLLAW+VMPRLLRRFHKYSMR PVA+LSGS S
Sbjct: 184 FTPHVDKLFDSHPYLKNVIVPVSMTLTATLLAWLVMPRLLRRFHKYSMRGPVALLSGSIS 243

Query: 241 IEEIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFI 300
           +EEIPYEKSFWGALEDPLRYLVTF+AFSQIG MVAPT +ASE+V+QACRGAVILSLVWFI
Sbjct: 244 VEEIPYEKSFWGALEDPLRYLVTFFAFSQIGMMVAPTTVASEYVTQACRGAVILSLVWFI 303

Query: 301 YRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGG 360
           YRWKTNV SRALASQ+FSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGG
Sbjct: 304 YRWKTNVLSRALASQSFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGG 363

Query: 361 IGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKF 420
           IGGVA AFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKF
Sbjct: 364 IGGVAAAFAARDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKF 423

Query: 421 PIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK----- 480
           PIVVPNSLFSSQVI+NKSRAQWRAITKKIP QV +LSTVS+ISD+IKSMLRSHPK     
Sbjct: 424 PIVVPNSLFSSQVIVNKSRAQWRAITKKIPLQVDNLSTVSQISDNIKSMLRSHPKVFLGK 483

Query: 481 --------------------------GKEELFSTEQDILLQSVQIMKENGATLGN 503
                                      K+ELFSTEQDILLQSVQI+KE+GATLG+
Sbjct: 484 EAPYCFLSQIESTYAELTIGCNLRKMSKDELFSTEQDILLQSVQIIKEHGATLGS 538

BLAST of CmoCh12G008810 vs. ExPASy TrEMBL
Match: A0A1S3AVZ3 (mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483257 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.6e-211
Identity = 395/514 (76.85%), Postives = 425/514 (82.68%), Query Frame = 0

Query: 20  CSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNVSRTGFLRAPSVCCLHTPSY 79
           CSFQ  K YTKIS+YV+RVLR +VVLNK+YY DESHS K           +VC LH PSY
Sbjct: 9   CSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRA---------TVCGLHIPSY 68

Query: 80  GTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKADITGDTGVPLASSGVEAD 139
           GTH   TF PAS +KYWNTIP TS+G LSN R YSSTS RKADITGDTGVP AS+GVE D
Sbjct: 69  GTHRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPD 128

Query: 140 ISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAP 199
           ISNPPDVGRD LE+VKD WQS VDAASFTGQKA+EVSDE SPHVDKLLDSHPYLKN I P
Sbjct: 129 ISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVP 188

Query: 200 VGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYL 259
           V MTL  TLLAW VMPRLLRRFHKYSMRSPVAI+SG+   EEIPYEKSFWGALEDPLRYL
Sbjct: 189 VSMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYL 248

Query: 260 VTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLD 319
           VTF+AFSQIG +VAPTA+A EFVSQACRGAVILSLVWFIYRWKTNVFSRALA+Q  +GLD
Sbjct: 249 VTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLD 308

Query: 320 REKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGL 379
           R++LLTLDKVSSV LFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGN+LSGL
Sbjct: 309 RDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGL 368

Query: 380 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQ 439
           TMQFSQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFP+VVPNSLFSSQVIINKSRAQ
Sbjct: 369 TMQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQ 428

Query: 440 WRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK-------------------------- 499
           WRAI K++P QV D+STVS+I+DDIK ML SHPK                          
Sbjct: 429 WRAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGC 488

Query: 500 -----GKEELFSTEQDILLQSVQIMKENGATLGN 503
                GK+E FSTEQDILLQSVQI+K  GATLG+
Sbjct: 489 NLKKMGKDESFSTEQDILLQSVQIIKARGATLGS 513

BLAST of CmoCh12G008810 vs. ExPASy TrEMBL
Match: A0A5A7U5P8 (Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001760 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.6e-211
Identity = 395/514 (76.85%), Postives = 425/514 (82.68%), Query Frame = 0

Query: 20  CSFQYCKSYTKISEYVNRVLRLSVVLNKQYYKDESHSLKNVSRTGFLRAPSVCCLHTPSY 79
           CSFQ  K YTKIS+YV+RVLR +VVLNK+YY DESHS K           +VC LH PSY
Sbjct: 40  CSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRA---------TVCGLHIPSY 99

Query: 80  GTHPAATFSPASPVKYWNTIPPTSLGYLSNPRFYSSTSSRKADITGDTGVPLASSGVEAD 139
           GTH   TF PAS +KYWNTIP TS+G LSN R YSSTS RKADITGDTGVP AS+GVE D
Sbjct: 100 GTHRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPD 159

Query: 140 ISNPPDVGRDWLERVKDGWQSVVDAASFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAP 199
           ISNPPDVGRD LE+VKD WQS VDAASFTGQKA+EVSDE SPHVDKLLDSHPYLKN I P
Sbjct: 160 ISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVP 219

Query: 200 VGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYL 259
           V MTL  TLLAW VMPRLLRRFHKYSMRSPVAI+SG+   EEIPYEKSFWGALEDPLRYL
Sbjct: 220 VSMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYL 279

Query: 260 VTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLD 319
           VTF+AFSQIG +VAPTA+A EFVSQACRGAVILSLVWFIYRWKTNVFSRALA+Q  +GLD
Sbjct: 280 VTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLD 339

Query: 320 REKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGL 379
           R++LLTLDKVSSV LFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGN+LSGL
Sbjct: 340 RDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGL 399

Query: 380 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQ 439
           TMQFSQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFP+VVPNSLFSSQVIINKSRAQ
Sbjct: 400 TMQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQ 459

Query: 440 WRAITKKIPFQVADLSTVSKISDDIKSMLRSHPK-------------------------- 499
           WRAI K++P QV D+STVS+I+DDIK ML SHPK                          
Sbjct: 460 WRAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGC 519

Query: 500 -----GKEELFSTEQDILLQSVQIMKENGATLGN 503
                GK+E FSTEQDILLQSVQI+K  GATLG+
Sbjct: 520 NLKKMGKDESFSTEQDILLQSVQIIKARGATLGS 544

BLAST of CmoCh12G008810 vs. TAIR 10
Match: AT4G00290.1 (Mechanosensitive ion channel protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.4e-135
Identity = 258/444 (58.11%), Postives = 328/444 (73.87%), Query Frame = 0

Query: 90  ASPVKYWNTIPPTS-LGYLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGR 149
           +S ++  N   P + LG+ S  R +SS    K+D  G     + +SGV     +    G 
Sbjct: 55  SSNIRLHNDAKPFNYLGHSSYARAFSS----KSDDFGS----IVASGVTGS-GDGNGNGN 114

Query: 150 DWLERVKDGWQSVVDAASFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAPVGMTLTATL 209
           DW+E+ KD  Q+ VDA + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL
Sbjct: 115 DWVEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTL 174

Query: 210 LAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQI 269
            AWVVMPR+LRRFH Y+M+S   +L    S E++PYEKSFWGALEDP RYLVTF AF+QI
Sbjct: 175 FAWVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQI 234

Query: 270 GKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDK 329
             MVAPT IA+++ S   +GAVILSLVWF+YRWKTNV +R L++++F GLDREK+LTLDK
Sbjct: 235 AAMVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDK 294

Query: 330 VSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFS 389
           VSSVGLFAIGLMA AEA GVAVQS+LTVGG+GGVATAFAARDILGNVLSGL+MQFS+PFS
Sbjct: 295 VSSVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFS 354

Query: 390 LGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIP 449
           +GDTIKAG++EGQV+EMGL TTSLL+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIP
Sbjct: 355 MGDTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIP 414

Query: 450 FQVADLSTVSKISDDIKSMLRSHPK-------------------------------GKEE 502
            Q+ DL  + +IS++IK MLRS+ K                               GKEE
Sbjct: 415 LQIDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEE 474

BLAST of CmoCh12G008810 vs. TAIR 10
Match: AT4G00234.1 (BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein (TAIR:AT4G00290.1); Has 57 Blast hits to 57 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 56; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 279.3 bits (713), Expect = 6.3e-75
Identity = 145/252 (57.54%), Postives = 186/252 (73.81%), Query Frame = 0

Query: 106 YLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSVVDAA 165
           YLS+  +  + SS+  D        + +SGV     +    G DW+E+ KD  Q+ V+A 
Sbjct: 19  YLSHSSYARAFSSKSDDFGS-----IVASGV---TGSGDGNGNDWVEKAKDVVQTSVNAV 78

Query: 166 SFTGQKAREVSDEFSPHVDKLLDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYS 225
           + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL AW+VMPR+LRRFH Y+
Sbjct: 79  AETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTVTGTLFAWIVMPRILRRFHTYA 138

Query: 226 MRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQA 285
           M+S   +L    S E+IPYEKSFWGALEDP RYLVTF AF+QI  MVAPT IA+++ S  
Sbjct: 139 MQSSAKLLPVGFSNEDIPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPT 198

Query: 286 CRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEA 345
            +GAVILS+VWF+YRWKTNV +R L++++F GLDR+K+LTLDKVSSVGLFAIGLMA AEA
Sbjct: 199 VKGAVILSVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLDKVSSVGLFAIGLMASAEA 258

Query: 346 SGVAVQSMLTVG 358
            GVAVQS+LTVG
Sbjct: 259 CGVAVQSILTVG 262

BLAST of CmoCh12G008810 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 55.1 bits (131), Expect = 1.9e-07
Identity = 40/140 (28.57%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 344 EASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTI--KAGAIE-- 403
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 404 GQVVEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPFQVADLSTV 463
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   +     D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 464 SKISDDIKSMLRSHPKGKEE 478
           + I  D++ +L  +P+ +++
Sbjct: 377 NNIVADMRKVLSKNPQIEQQ 395

BLAST of CmoCh12G008810 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 55.1 bits (131), Expect = 1.9e-07
Identity = 40/140 (28.57%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 344 EASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSLGDTI--KAGAIE-- 403
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 404 GQVVEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPFQVADLSTV 463
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   +     D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 464 SKISDDIKSMLRSHPKGKEE 478
           + I  D++ +L  +P+ +++
Sbjct: 377 NNIVADMRKVLSKNPQIEQQ 395

BLAST of CmoCh12G008810 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 52.0 bits (123), Expect = 1.6e-06
Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 330 SSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNVLSGLTMQFSQPFSL 389
           S+V + A+ L    E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L
Sbjct: 243 SAVWVAAVSL--FMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 302

Query: 390 GD----TIKAGAIEGQVVEMGLMTTSLLSAE-KFPIVVPNSLFSSQVIIN-KSRAQWRAI 449
            +     I+   + G V  +G  + +++  E +  I +PN  F+  V+ N   +  WR I
Sbjct: 303 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWR-I 362

Query: 450 TKKIPFQVADLSTVSKISDDIKSMLRSHPKGKEE 478
              +     D++ ++ I  D++ +L  +P  +++
Sbjct: 363 KTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQ 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZL43.4e-13458.11Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX... [more]
P0AEB64.8e-1933.87Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB54.8e-1933.87Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
O251702.3e-1329.74Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC ... [more]
Q9ZKG73.0e-1329.74Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FHY51.4e-27694.18mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita moschata... [more]
A0A6J1I1Q48.9e-27192.12mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima O... [more]
A0A6J1D2804.2e-22077.38mechanosensitive ion channel protein 1, mitochondrial-like OS=Momordica charanti... [more]
A0A1S3AVZ31.6e-21176.85mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN... [more]
A0A5A7U5P81.6e-21176.85Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT4G00290.12.4e-13558.11Mechanosensitive ion channel protein [more]
AT4G00234.16.3e-7557.54BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein... [more]
AT1G58200.11.9e-0728.57MSCS-like 3 [more]
AT1G58200.21.9e-0728.57MSCS-like 3 [more]
AT5G10490.11.6e-0627.27MSCS-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.1260coord: 282..384
e-value: 1.5E-14
score: 56.1
NoneNo IPR availablePANTHERPTHR30566YNAI-RELATED MECHANOSENSITIVE ION CHANNELcoord: 91..473
NoneNo IPR availablePANTHERPTHR30566:SF5MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATEDcoord: 91..473
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 386..434
e-value: 3.2E-12
score: 48.2
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 328..475
e-value: 7.4E-30
score: 104.0
IPR011014Mechanosensitive ion channel MscS, transmembrane-2SUPERFAMILY82861Mechanosensitive channel protein MscS (YggB), transmembrane regioncoord: 287..369
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 370..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G008810.1CmoCh12G008810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane