CmoCh12G007290 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G007290
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Cytochrome b559 subunit alpha
Location	Cmo_Chr12: 5639213 .. 5639972 (+)
RNA-Seq Expression	CmoCh12G007290
Synteny	CmoCh12G007290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAGTTCAGCAGGAAGCACAGGAGAACGTTCTTTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGTGGGTTGGTTTAGCTTACGATGTTTTTGGCGGCCCTTGTCCAACTGAGTATTTTACAGAGAGCCGACAAGGAATTCCTTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCTATGAATTTAGTAGATCTTTTTAAAAATGACTATCGATCAAACTTATCCTATTTTGACAGTGCGATGGTTGGCTATTCATGGACTAGCTGTACCTACCCTTTCTTTTTTAGGGGCAATATCAGCAATGCAGTTCACGAGAAAGCTAATCCGAATTATCGAGCTATGACACAATCAAATCCGAACGAACACAATGTTGAATGGAATCGTACTTGTCTTTACTGGGTGGGGGTTATTCCTAATTTTTGTACTTGCTCTTTGATTTTTCAATTCTTTCTTCAATAAAAGAAAAGACTAAATAATAGTACGAATTATCTTATCCCACTCGGAAGGATATCATCTCATAATATGACTCTTTCCGTCTGCCGCACGACCACTTGATGAAATGGGGAGGGAAGTGGGACAAATGGCCGATCCTACTGTAAGGATTCATTCCTCTTTGTATAATAGGTACTGTCAAAGGTATTCCTGTGATCGGTTTAGTCGGTATTTTCTTTTATGGTTCATATTCTGGATTAGGTTCATCTCTGTAA

mRNA sequence

ATGTGGAGTTCAGCAGGAAGCACAGGAGAACGTTCTTTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGTGGGTTGGTTTAGCTTACGATGTTTTTGGCGGCCCTTGTCCAACTGAGTATTTTACAGAGAGCCGACAAGGAATTCCTTCCATTAATAACTGGCCGGGCAATATCAGCAATGCAGTTCACGAGAAAGCTAATCCGAATTATCGAGCTATGACACAATCAAATCCGAACGAACACAATGTTGAATGGAATCGTACTGTCAAAGGTATTCCTGTGATCGGTTTAGTCGGTATTTTCTTTTATGGTTCATATTCTGGATTAGGTTCATCTCTGTAA

Coding sequence (CDS)

ATGTGGAGTTCAGCAGGAAGCACAGGAGAACGTTCTTTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGTGGGTTGGTTTAGCTTACGATGTTTTTGGCGGCCCTTGTCCAACTGAGTATTTTACAGAGAGCCGACAAGGAATTCCTTCCATTAATAACTGGCCGGGCAATATCAGCAATGCAGTTCACGAGAAAGCTAATCCGAATTATCGAGCTATGACACAATCAAATCCGAACGAACACAATGTTGAATGGAATCGTACTGTCAAAGGTATTCCTGTGATCGGTTTAGTCGGTATTTTCTTTTATGGTTCATATTCTGGATTAGGTTCATCTCTGTAA

Protein sequence

MWSSAGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWVGLAYDVFGGPCPTEYFTESRQGIPSINNWPGNISNAVHEKANPNYRAMTQSNPNEHNVEWNRTVKGIPVIGLVGIFFYGSYSGLGSSL

Homology

[Close]

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot
Match: Q3V518 (Cytochrome b559 subunit alpha OS=Acorus calamus OX=4465 GN=psbE PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 35/65 (53.85%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSI 66
          GIP I
Sbjct: 62 GIPLI 66

[Close]

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot
Match: A1EA25 (Cytochrome b559 subunit alpha OS=Agrostis stolonifera OX=63632 GN=psbE PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 35/67 (52.24%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSINN 68
          GIP I +
Sbjct: 62 GIPLITD 68

[Close]

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot
Match: Q70XY9 (Cytochrome b559 subunit alpha OS=Amborella trichopoda OX=13333 GN=psbE PE=3 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 35/65 (53.85%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSI 66
          GIP I
Sbjct: 62 GIPLI 66

[Close]

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot
Match: P56779 (Cytochrome b559 subunit alpha OS=Arabidopsis thaliana OX=3702 GN=psbE PE=1 SV=4)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 35/65 (53.85%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSI 66
          GIP I
Sbjct: 62 GIPLI 66

[Close]

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot
Match: P59702 (Cytochrome b559 subunit alpha OS=Atropa belladonna OX=33113 GN=psbE PE=3 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 35/65 (53.85%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSI 66
          GIP I
Sbjct: 62 GIPLI 66

[Close]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL
Match: A0A7J6HZU7 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_004365 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.4e-16
Identity = 76/192 (39.58%), Postives = 87/192 (45.31%), Query Frame = 0


Query: 5   AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
           +GSTGERS   +I S        I +  L  + W+    GLAYDVFG P P E FTESRQ
Sbjct: 81  SGSTGERSLADIITSIRDWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNECFTESRQ 140

Query: 65  GIPSINN---------------WPGNIS------------NAVH--------------EK 124
           G+P I                 W   ++             AVH              +K
Sbjct: 141 GVPLITGRFDSLEQLDEFSRSFWEAQMTIDRTYPIFTVRWLAVHGLVVPTVSFLGSSNDK 200

Query: 125 ANPNYRAMTQSNPNEHNVEWNR------------------------TVKGIPVIGLVGIF 128
            N N+RA TQSNPNE NVE NR                        TV GI VIGL+GIF
Sbjct: 201 PNSNHRATTQSNPNEQNVELNRTSLYWGKVGQMADTTGRIPLWIIGTVAGILVIGLLGIF 260

[Close]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL
Match: G7JFY7 (Cytochrome b559 subunit alpha OS=Medicago truncatula OX=3880 GN=MTR_4g050850 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-14
Identity = 58/129 (44.96%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 5   AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
           +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2   SGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65  GIPSINNWPGNIS-----------NAVHEKANPNYRAMTQSNPNEHNVEWNRT--VKGIP 117
           GIP I     ++            ++ ++K NPNYRA TQSNPNE NVE NRT    G+ 
Sbjct: 62  GIPLITGRFDSLEQLDEFIRWLQCSSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLL 121

[Close]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL
Match: A0A7J6HPJ5 (Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019796 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.8e-14
Identity = 77/222 (34.68%), Postives = 88/222 (39.64%), Query Frame = 0


Query: 5   AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
           +GSTGERS   +I S        I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 81  SGSTGERSLADIITSIRDWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 140

Query: 65  GIPSINN---------------WPGNIS------------NAVH--------------EK 124
           G+P I                 W   ++             AVH              +K
Sbjct: 141 GVPLITGRFDSLEQLDEFSRSFWEAQMTIDRTYPIFTVRWLAVHGLAVPTVSFLGSSNDK 200

Query: 125 ANPNYRAMTQSNPNEHNVEWNR-------------------------------------- 128
            NPN+RA TQ NPNE NVE NR                                      
Sbjct: 201 PNPNHRATTQPNPNEQNVELNRTSLYWGNSLIPFGRISSHNYLWLFMSLARPPDEMWRKV 260

[Close]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL
Match: A0A3Q7X043 (Cytochrome b559 subunit alpha OS=Cicer arietinum OX=3827 GN=LOC113785018 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.1e-13
Identity = 61/146 (41.78%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 5   AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
           +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2   SGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65  GIPSINNWPGNISN--------AVH--------------------EKANPNYRAMTQSNP 117
           GIP I     ++          AVH                    +K NPNYRA TQSNP
Sbjct: 62  GIPLITGRFDSLEQLDEFIRWLAVHGLAVPTVLFWDRYQQCSSSNDKPNPNYRATTQSNP 121

[Close]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL
Match: A0A7J6HPK4 (Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019806 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.6e-13
Identity = 60/130 (46.15%), Postives = 72/130 (55.38%), Query Frame = 0


Query: 6   GSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQG 65
           GS GERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQG
Sbjct: 82  GSIGERSFADIITSIRYWVIHSITIPSLFITGWLFVSTGLAYDVFGSPRPNEYFTESRQG 141

Query: 66  IPSINNWPGNISNAVHEKANPN--YRAMTQSNPNEHNVE-W-NRTVKGIPVIGLVGIFFY 125
           +P I      +         P+  +R + Q       +  W   TV GI VIGL+GIFFY
Sbjct: 142 VPLITGRFDYLEQLDEFTRQPDEMWRKVGQMADTTGRIPLWIIGTVAGILVIGLLGIFFY 201

Query: 126 GSYSGLGSSL 128
           GSYSGLGSSL
Sbjct: 202 GSYSGLGSSL 211

[Close]

BLAST of CmoCh12G007290 vs. TAIR 10
Match: ATCG00580.1 (photosystem II reaction center protein E )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 35/65 (53.85%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 5  AGSTGERSFLLLILSPVFDTGSFIALLYLLYSLWV----GLAYDVFGGPCPTEYFTESRQ 64
          +GSTGERSF  +I S  +     I +  L  + W+    GLAYDVFG P P EYFTESRQ
Sbjct: 2  SGSTGERSFADIITSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQ 61

Query: 65 GIPSI 66
          GIP I
Sbjct: 62 GIPLI 66

[Close]

BLAST of CmoCh12G007290 vs. TAIR 10
Match: ATCG00550.1 (photosystem II reaction center protein J )

HSP 1 Score: 48.1 bits (113), Expect = 5.9e-06
Identity = 23/26 (88.46%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 102 TVKGIPVIGLVGIFFYGSYSGLGSSL 128
           TV GI VIGL+GIFFYGSYSGLGSSL
Sbjct: 15  TVAGILVIGLIGIFFYGSYSGLGSSL 40

The following BLAST results are available for this feature:

BLAST of CmoCh12G007290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3V518	2.8e-08	53.85	Cytochrome b559 subunit alpha OS=Acorus calamus OX=4465 GN=psbE PE=3 SV=1	[more]
A1EA25	2.8e-08	52.24	Cytochrome b559 subunit alpha OS=Agrostis stolonifera OX=63632 GN=psbE PE=3 SV=1	[more]
Q70XY9	2.8e-08	53.85	Cytochrome b559 subunit alpha OS=Amborella trichopoda OX=13333 GN=psbE PE=3 SV=3	[more]
P56779	2.8e-08	53.85	Cytochrome b559 subunit alpha OS=Arabidopsis thaliana OX=3702 GN=psbE PE=1 SV=4	[more]
P59702	2.8e-08	53.85	Cytochrome b559 subunit alpha OS=Atropa belladonna OX=33113 GN=psbE PE=3 SV=3	[more]

BLAST of CmoCh12G007290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7J6HZU7	6.4e-16	39.58	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_004365 PE=3 SV=1	[more]
G7JFY7	1.6e-14	44.96	Cytochrome b559 subunit alpha OS=Medicago truncatula OX=3880 GN=MTR_4g050850 PE=...	[more]
A0A7J6HPJ5	7.8e-14	34.68	Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019796 PE=3 SV...	[more]
A0A3Q7X043	5.1e-13	41.78	Cytochrome b559 subunit alpha OS=Cicer arietinum OX=3827 GN=LOC113785018 PE=3 SV...	[more]
A0A7J6HPK4	6.6e-13	46.15	Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019806 PE=3 SV...	[more]

BLAST of CmoCh12G007290 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00580.1	2.0e-09	53.85	photosystem II reaction center protein E	[more]
ATCG00550.1	5.9e-06	88.46	photosystem II reaction center protein J	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037025	Photosystem II cytochrome b559, alpha subunit superfamily	GENE3D	1.20.5.860	Photosystem II cytochrome b559, alpha subunit	coord: 4..70 e-value: 2.6E-13 score: 51.6
IPR013082	Photosystem II cytochrome b559, alpha subunit, lumenal region	PFAM	PF00284	Cytochrom_B559a	coord: 41..66 e-value: 8.0E-10 score: 38.3
None	No IPR available	GENE3D	6.10.250.2070		coord: 101..127 e-value: 1.6E-12 score: 48.9
None	No IPR available	SUPERFAMILY	161045	Cytochrome b559 subunits	coord: 6..67
IPR002682	Photosystem II PsbJ	PFAM	PF01788	PsbJ	coord: 102..127 e-value: 7.9E-12 score: 44.9
IPR037267	Photosystem II PsbJ superfamily	SUPERFAMILY	161021	Photosystem II reaction center protein J, PsbJ	coord: 102..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G007290.1	CmoCh12G007290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009767	photosynthetic electron transport chain
biological_process	GO:0015979	photosynthesis
biological_process	GO:0019684	photosynthesis, light reaction
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043229	intracellular organelle
cellular_component	GO:0009539	photosystem II reaction center
cellular_component	GO:0016020	membrane
cellular_component	GO:0009523	photosystem II
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding