CmoCh12G005790 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G005790
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionzinc transporter 4, chloroplastic-like
LocationCmo_Chr12: 3584802 .. 3587060 (-)
RNA-Seq ExpressionCmoCh12G005790
SyntenyCmoCh12G005790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCAAGAAGTCATAACTGTAGGCTCATGCCTCTGCGTCTGCCCTCCATTTCATTCCATTATGTCGACACTCTTCTCTCTTCCCTTCTCCCCCATCTCCCACCTTCTTTCTCTCTTCTCCTCCTTCTCCGATCGCTCGCGGCCGCTTCTCTTTTATAAACTCTTCAAACAATCCCCCTTCCATCCTCATTCCATTTCCGGCGCCTTTTCTTTTCACCATTGTAATCTATTCTCATGCTGTTCTTCGAGGTTCCAATCCCTCCCCATTCTTCCCTCTTTGGTTTCTCTGATTTCTCCTCGTTTATATGTGTTCTGTTCTTCAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGGTAATTTCTCCATTCTCGTTTGTTTTCGCCTTGATTCGTCTATTCCGCTTTCAATTGAACTGAGTCTGTTTCAGAAATCTGTGGATTCCTAATTTCTTGGTGTTTCTGTATTCTTGTTATTGATGGATTAATTTATTGATTCTTCGTTTGTAGGTTTTAGTTAGATTAGGTTTGTGTAATTGATTTCTAATCTGGGGAATTTTCGTTCTCCATTTGTAATCGAACAGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCCGGAGTTTTGGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCGGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAGTTTCCATGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTCGTCGATTTCGTTGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATTCCGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGTCACAGTCATCCGCAGGGGAAGGATGCATGTGACGGGCACGTGACACTCCATGATCATGGCCATGGTCATTCTCATGGGCTGGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGGTAATCCATTTATCTTCTCGATTAATGAAAATTCCCGAATATTGACGTCATTATTCTATGGGACAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTTGGAGTGTCCCACAGCCCATGCACCATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCTCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTTTTCGCCGCCACCACGCCCGTCGGGATTGCCATTGGCGCGGCGATCTCTTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATACTGGTGTATATGGCTTTGGTTGACTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACGCTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGATCATAAATTCTATTCGTTTTTTTGGGAGGAAAATGTTTTTTCGACGTTGTTAGGAGGAAGAAATGGAAAGAGATAGATGATGTTAGGGAATTAATTGTTTTTGGAAGGGTATGTACACTCGAAATCCCTTTTGGAATTCCACAAAAATGCATCAACTGACCTAATGTCTTAACTAGTTGAGTTACGCTACGTCATGTTGTAAGTTAATAATAAATTTGGTTTTCAAACATTTAAACTTTTGGCACTAGCTTGCACGTCGTCTAGACATGGATTACAACGTTTAGATCACATGGGTAAGTTGACCTCTTTGATGATTGGAGTTTGTTTATCTCTACTAAAATTTTTATGCCTAAATGGCATTATCAGAATATCCGTCAAGAATGAGCGTTCAATTGTTCATGCATGGCCTCATTTGCAAATCAT

mRNA sequence

AAGCAAGAAGTCATAACTGTAGGCTCATGCCTCTGCGTCTGCCCTCCATTTCATTCCATTATGTCGACACTCTTCTCTCTTCCCTTCTCCCCCATCTCCCACCTTCTTTCTCTCTTCTCCTCCTTCTCCGATCGCTCGCGGCCGCTTCTCTTTTATAAACTCTTCAAACAATCCCCCTTCCATCCTCATTCCATTTCCGGCGCCTTTTCTTTTCACCATTGTAATCTATTCTCATGCTGTTCTTCGAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCCGGAGTTTTGGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCGGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAGTTTCCATGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTCGTCGATTTCGTTGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATTCCGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGTCACAGTCATCCGCAGGGGAAGGATGCATGTGACGGGCACGTGACACTCCATGATCATGGCCATGGTCATTCTCATGGGCTGGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTTGGAGTGTCCCACAGCCCATGCACCATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCTCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTTTTCGCCGCCACCACGCCCGTCGGGATTGCCATTGGCGCGGCGATCTCTTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATACTGGTGTATATGGCTTTGGTTGACTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACGCTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGATCATAAATTCTATTCGTTTTTTTGGGAGGAAAATGTTTTTTCGACGTTGTTAGGAGGAAGAAATGGAAAGAGATAGATGATGTTAGGGAATTAATTGTTTTTGGAAGGGTATGTACACTCGAAATCCCTTTTGGAATTCCACAAAAATGCATCAACTGACCTAATGTCTTAACTAGTTGAGTTACGCTACGTCATGTTGTAAGTTAATAATAAATTTGGTTTTCAAACATTTAAACTTTTGGCACTAGCTTGCACGTCGTCTAGACATGGATTACAACGTTTAGATCACATGGGTAAGTTGACCTCTTTGATGATTGGAGTTTGTTTATCTCTACTAAAATTTTTATGCCTAAATGGCATTATCAGAATATCCGTCAAGAATGAGCGTTCAATTGTTCATGCATGGCCTCATTTGCAAATCAT

Coding sequence (CDS)

ATGCTGTTCTTCGAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCCGGAGTTTTGGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCGGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAGTTTCCATGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTCGTCGATTTCGTTGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATTCCGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGTCACAGTCATCCGCAGGGGAAGGATGCATGTGACGGGCACGTGACACTCCATGATCATGGCCATGGTCATTCTCATGGGCTGGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTTGGAGTGTCCCACAGCCCATGCACCATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCTCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTTTTCGCCGCCACCACGCCCGTCGGGATTGCCATTGGCGCGGCGATCTCTTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATACTGGTGTATATGGCTTTGGTTGACTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACGCTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGA

Protein sequence

MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Homology
BLAST of CmoCh12G005790 vs. ExPASy Swiss-Prot
Match: Q8LE59 (Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT3 PE=2 SV=3)

HSP 1 Score: 509.2 bits (1310), Expect = 4.3e-143
Identity = 273/426 (64.08%), Postives = 323/426 (75.82%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLS--ESIVRTVSESMGNSS----CSVSELELCRDDSAA 60
           M F + LW+++    F  +T+ LS  ESI++ V E+M  +S    C+ SE +LCRDDSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV  KAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ +    E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQ-EREASESVEPFG 180

Query: 181 SGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTL- 240
                G+   ++    ++ KVFGEED GG+HIVG+HAHAA HRHSHP G D+C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 --------HDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
                   H HGHGH H   G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 412
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of CmoCh12G005790 vs. ExPASy Swiss-Prot
Match: O04089 (Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.2e-138
Identity = 264/376 (70.21%), Postives = 297/376 (78.99%), Query Frame = 0

Query: 40  CSVSELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFA 99
           C   E +LCRDDSAA   K +AIASIL +G  G+AIPL GR+RRFL+T+GNLFVA KAFA
Sbjct: 10  CDAGESDLCRDDSAAFLLKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFA 69

Query: 100 AGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYER 159
           AGVILAT FVHML  G++ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYER
Sbjct: 70  AGVILATGFVHMLAGGTEALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYER 129

Query: 160 KQGQIRLKEETNRVGSGLDSGL-EAGILPAADSNV---KVFGEEDGGGMHIVGMHAHAAQ 219
           KQ       E N+  +   +G  E  ++P     V   KVFGEEDGGG+HIVG+ AHAA 
Sbjct: 130 KQ-------ERNQAATEAAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAH 189

Query: 220 HRHSHPQGKDACDGHVTLHDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGL 279
           HRHSH      CDGH   H H HGH HG    D E+G RHVVVSQILELGIVSHS+IIGL
Sbjct: 190 HRHSHSNSHGTCDGHA--HGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSIIIGL 249

Query: 280 SLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAI 339
           SLGVS SPCTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI I
Sbjct: 250 SLGVSQSPCTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGI 309

Query: 340 GAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYC 399
           G A++S++N +S GALV EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+VSY 
Sbjct: 310 GTAVASSFNSHSPGALVTEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYV 369

Query: 400 TLFLGAGLMSLLALWA 412
            LFLGAGLMS LA+WA
Sbjct: 370 MLFLGAGLMSALAIWA 374

BLAST of CmoCh12G005790 vs. ExPASy Swiss-Prot
Match: Q5Z653 (Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2)

HSP 1 Score: 411.8 bits (1057), Expect = 9.3e-114
Identity = 234/401 (58.35%), Postives = 273/401 (68.08%), Query Frame = 0

Query: 28  VRTVSESMGNSSCSV------SELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRH 87
           +R ++ S+  +SC         + E CRD++AAL  K++A+A+IL +G  G+AIPL GR 
Sbjct: 13  IRQIAASVSAASCDAVVGGGGDKDEECRDEAAALRLKMVAVAAILIAGAAGVAIPLVGRR 72

Query: 88  RR-------FLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPF 147
           RR          + G LFV  KAFAAGVILAT FVHML D   ALS+PCLP  PW +FPF
Sbjct: 73  RRGGGGGGGGGASSGGLFVLAKAFAAGVILATGFVHMLHDAEHALSNPCLPHSPWRRFPF 132

Query: 148 SGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSGLEAGILPAADSNVK 207
            GF AM+A+L TL+VDFVGT +YERK  Q           + L    + G LP  D    
Sbjct: 133 PGFVAMLAALATLVVDFVGTHFYERKHRQEEAAAAAEEAAAALLE--DGGALPVGDGE-- 192

Query: 208 VFGEEDGGG----MHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGHSHGLDGDDDE 267
             G +  GG    MHIVG+HAHAA HRHSH     AC G      H HGH H   G ++ 
Sbjct: 193 --GRDGRGGKRDAMHIVGIHAHAAAHRHSHAHVHGACHGGAVNDAHAHGHGH---GHEEG 252

Query: 268 SGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQ 327
              RHVVVSQILELGIVSHSVIIGLSLGVS SPCTI+PL+AALSFHQFFEGFALGGCIS+
Sbjct: 253 PSARHVVVSQILELGIVSHSVIIGLSLGVSQSPCTIKPLVAALSFHQFFEGFALGGCISE 312

Query: 328 AQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMAL 387
           AQ    S  LMA FFA TTP GI +GAA++S YNPNS  ALV EGILDS+SAGIL+YMAL
Sbjct: 313 AQLKNFSAFLMAFFFAITTPAGITVGAAVASFYNPNSPRALVVEGILDSMSAGILIYMAL 372

Query: 388 VDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           VDLIAADFLS++MSCN RLQ+ SY  LFLGA  M+ LALWA
Sbjct: 373 VDLIAADFLSRKMSCNPRLQVGSYIALFLGAMAMAALALWA 404

BLAST of CmoCh12G005790 vs. ExPASy Swiss-Prot
Match: O82643 (Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 7.4e-111
Identity = 222/357 (62.18%), Postives = 266/357 (74.51%), Query Frame = 0

Query: 62  IASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G++IPL G     L  +G L    KAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGL---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ ++ + +    +G+ +    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKM-MNQSVESLGTQVSVMS 120

Query: 182 DSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHS---HPQGKDACDGHVTLH 241
           D GLE+G L           +EDGG +HIVGM AHA  HRHS     +G +A      + 
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 DHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 301
            HGHGHSHG      +SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL+ ALS
Sbjct: 181 GHGHGHSHGHGDVGLDSGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPLLLALS 240

Query: 302 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 361
           FHQFFEGFALGGC+++A+     +++MA FFA TTP+G+A+G AI+S+YN  S  ALVAE
Sbjct: 241 FHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVAALVAE 300

Query: 362 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           G+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS LA+WA
Sbjct: 301 GVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAIWA 344

BLAST of CmoCh12G005790 vs. ExPASy Swiss-Prot
Match: Q6L8F7 (Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 5.9e-100
Identity = 212/371 (57.14%), Postives = 252/371 (67.92%), Query Frame = 0

Query: 48  CRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATA 107
           CRDD+AAL  K +A+A+IL +GV+G+ +PL GR RR L+TD   FVA KAFAAGVILAT 
Sbjct: 36  CRDDAAALRLKGVAMATILVAGVVGVGLPLAGRKRRALRTDSAAFVAAKAFAAGVILATG 95

Query: 108 FVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLK 167
           FVHML D   ALS PCLP  PW  FPF GF AM A+L TL++DF+ T++YE K      +
Sbjct: 96  FVHMLHDAEHALSSPCLPAHPWRSFPFPGFVAMSAALATLVLDFLATRFYEGKH-----R 155

Query: 168 EETNRVGSGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDAC 227
            ET RV +   + L A      D  V    E+D      + +  H+  H HSHP      
Sbjct: 156 AETERVKAAAAAALAASSASDDDITVVTVTEDDNDNKAPL-LQPHS--HSHSHP------ 215

Query: 228 DGHVTLHDHGHGHSHGLDGDDDESG-------VRHVVVSQILELGIVSHSVIIGLSLGVS 287
                   HGHGH H L   +   G       VR VVVSQILE+GIVSHSVIIGLSLGVS
Sbjct: 216 --------HGHGHGHELAQPEGSGGEGEVPAQVRSVVVSQILEMGIVSHSVIIGLSLGVS 275

Query: 288 HSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAIS 347
            SPCTIRPL+AALSFHQFFEGFALGGCI+QAQF TLS ++MA FFA TTP GIA GA ++
Sbjct: 276 RSPCTIRPLVAALSFHQFFEGFALGGCIAQAQFKTLSAAIMACFFAITTPAGIAAGAGVA 335

Query: 348 STYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLG 407
           S YN NS  ALV EGILDS+SAGIL+YM+LVDLIAADFL  +M+ + R Q+++Y  LFLG
Sbjct: 336 SFYNANSPRALVVEGILDSVSAGILIYMSLVDLIAADFLGGKMTGSTRQQVMAYIALFLG 384

Query: 408 AGLMSLLALWA 412
           A  MS LA+WA
Sbjct: 396 ALSMSSLAIWA 384

BLAST of CmoCh12G005790 vs. ExPASy TrEMBL
Match: A0A6J1GGQ1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111454070 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 8.5e-227
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmoCh12G005790 vs. ExPASy TrEMBL
Match: A0A6J1KKC0 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496508 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.4e-224
Identity = 407/411 (99.03%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLD G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGK+ACDGHVTLHDH HGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmoCh12G005790 vs. ExPASy TrEMBL
Match: A0A6J1HFN1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462610 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 8.5e-203
Identity = 369/414 (89.13%), Postives = 386/414 (93.24%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M F E+LWQML  +GFQD+ R+LSES++RTVSESMGNS C VSELELCRDDSAA +FKLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GIAIPLFG  RRFLKTDGNLFV  KAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS  DSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDH---G 240
           LE+GILPA D NVKVFGEEDGG MHI+GMHAHAA HRHSHPQGK+ACDGHV +HDH   G
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHGG 240

Query: 241 HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ 300
           HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ
Sbjct: 241 HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ 300

Query: 301 FFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGIL 360
           FFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIAIG A++STYNPNSTGAL+AEGIL
Sbjct: 301 FFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAEGIL 360

Query: 361 DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of CmoCh12G005790 vs. ExPASy TrEMBL
Match: A0A0A0LKS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 4.2e-202
Identity = 368/417 (88.25%), Postives = 391/417 (93.76%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M FFEDL QML   GFQD+TRLLSES +RTVSESM NS+C VSELELCR+DSAAL+FKLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILT+GV+GIA+PLFG+ RRFLKTDGNLFVA KAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS +D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHD----- 240
           LE+GI+PA D NVKVFGEE+GGGMHIVGMHAHAA HRH+HPQGK+ACDGH+ +HD     
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 -HGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
            HGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIA+GAA++STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCTLFLGAGLMS LALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of CmoCh12G005790 vs. ExPASy TrEMBL
Match: A0A6J1K6U3 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491704 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 2.7e-201
Identity = 367/416 (88.22%), Postives = 386/416 (92.79%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M F EDLWQ+L  +GFQD+ R+LSES++RTVSESMGNS C VSELELCRDDSAA +FKLI
Sbjct: 1   MFFLEDLWQILCVQGFQDKARVLSESLLRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GIAIPLFG  RRFLKTDGNLFVA KAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS +DSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVVDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDH---- 240
           LE+GILPA D NVK+FGEEDGG MHIVGMHAHA  HR SHPQGK+ACDGHV +HDH    
Sbjct: 181 LESGILPAGDLNVKLFGEEDGGAMHIVGMHAHAGHHRPSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 -GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
            GHGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 241 GGHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIAIGAA++STYNPNSTGAL+AEG
Sbjct: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGAAVASTYNPNSTGALIAEG 360

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of CmoCh12G005790 vs. TAIR 10
Match: AT1G60960.1 (iron regulated transporter 3 )

HSP 1 Score: 509.2 bits (1310), Expect = 3.0e-144
Identity = 273/426 (64.08%), Postives = 323/426 (75.82%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLS--ESIVRTVSESMGNSS----CSVSELELCRDDSAA 60
           M F + LW+++    F  +T+ LS  ESI++ V E+M  +S    C+ SE +LCRDDSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV  KAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ +    E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQ-EREASESVEPFG 180

Query: 181 SGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTL- 240
                G+   ++    ++ KVFGEED GG+HIVG+HAHAA HRHSHP G D+C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 --------HDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
                   H HGHGH H   G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 412
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of CmoCh12G005790 vs. TAIR 10
Match: AT1G10970.1 (zinc transporter 4 precursor )

HSP 1 Score: 507.7 bits (1306), Expect = 8.9e-144
Identity = 277/419 (66.11%), Postives = 320/419 (76.37%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSS----CSVSELELCRDDSAALN 60
           M+F + LW++     F      LSESI++ + E+M +S+    C   E +LCRDDSAA  
Sbjct: 1   MIFVDVLWKLFPLYSFGSGRDSLSESILQIIPETMASSTTKILCDAGESDLCRDDSAAFL 60

Query: 61  FKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGS 120
            K +AIASIL +G  G+AIPL GR+RRFL+T+GNLFVA KAFAAGVILAT FVHML  G+
Sbjct: 61  LKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGT 120

Query: 121 KALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSG 180
           +ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYERKQ       E N+  + 
Sbjct: 121 EALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYERKQ-------ERNQAATE 180

Query: 181 LDSGL-EAGILPAADSNV---KVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVT 240
             +G  E  ++P     V   KVFGEEDGGG+HIVG+ AHAA HRHSH      CDGH  
Sbjct: 181 AAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAHHRHSHSNSHGTCDGHA- 240

Query: 241 LHDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAA 300
            H H HGH HG    D E+G RHVVVSQILELGIVSHS+IIGLSLGVS SPCTIRPLIAA
Sbjct: 241 -HGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSIIIGLSLGVSQSPCTIRPLIAA 300

Query: 301 LSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALV 360
           LSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N +S GALV
Sbjct: 301 LSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGIGTAVASSFNSHSPGALV 360

Query: 361 AEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
            EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+VSY  LFLGAGLMS LA+WA
Sbjct: 361 TEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYVMLFLGAGLMSALAIWA 408

BLAST of CmoCh12G005790 vs. TAIR 10
Match: AT4G33020.1 (ZIP metal ion transporter family )

HSP 1 Score: 402.1 bits (1032), Expect = 5.3e-112
Identity = 222/357 (62.18%), Postives = 266/357 (74.51%), Query Frame = 0

Query: 62  IASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G++IPL G     L  +G L    KAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGL---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ ++ + +    +G+ +    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKM-MNQSVESLGTQVSVMS 120

Query: 182 DSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHS---HPQGKDACDGHVTLH 241
           D GLE+G L           +EDGG +HIVGM AHA  HRHS     +G +A      + 
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 DHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 301
            HGHGHSHG      +SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL+ ALS
Sbjct: 181 GHGHGHSHGHGDVGLDSGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPLLLALS 240

Query: 302 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 361
           FHQFFEGFALGGC+++A+     +++MA FFA TTP+G+A+G AI+S+YN  S  ALVAE
Sbjct: 241 FHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVAALVAE 300

Query: 362 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           G+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS LA+WA
Sbjct: 301 GVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAIWA 344

BLAST of CmoCh12G005790 vs. TAIR 10
Match: AT1G05300.1 (zinc transporter 5 precursor )

HSP 1 Score: 284.3 bits (726), Expect = 1.6e-76
Identity = 168/385 (43.64%), Postives = 236/385 (61.30%), Query Frame = 0

Query: 31  VSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGN 90
           ++ S G S C  S  +   + + A  +K+ AI S+L +GV+G+  PL G+    LK +  
Sbjct: 22  IAVSAGESKCECSHEDDEANKAGAKKYKIAAIPSVLAAGVIGVMFPLLGKFFPSLKPETT 81

Query: 91  LFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVD 150
            F  TKAFAAGVILAT F+H+LP+G + L+ PCL    W +FPF+GF AM+A++LTL VD
Sbjct: 82  FFFVTKAFAAGVILATGFMHVLPEGYEKLTSPCLKGEAW-EFPFTGFIAMVAAILTLSVD 141

Query: 151 FVGTQYYERKQGQIRLKEETNRVGSGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMH 210
              T Y+ +        + + R+G G +     G                GGG   +G+H
Sbjct: 142 SFATSYFHKAH-----FKTSKRIGDGEEQDAGGG----------------GGGGDELGLH 201

Query: 211 AHAAQHRHSHPQGKDACDGHVTLHDHGHGHSHGLDG-DDDESGV---RHVVVSQILELGI 270
            HA                        HGH+HG+ G +  ES V   R  VV+Q+LE+GI
Sbjct: 202 VHA------------------------HGHTHGIVGVESGESQVQLHRTRVVAQVLEVGI 261

Query: 271 VSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFA 330
           + HSV+IG+SLG S SP T + L AAL FHQ FEG  LGGCI+Q  FN +S ++M++FF+
Sbjct: 262 IVHSVVIGISLGASQSPDTAKALFAALMFHQCFEGLGLGGCIAQGNFNCMSITIMSIFFS 321

Query: 331 ATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCN 390
            TTPVGIA+G AISS+Y+ +S  AL+ +G+L++ SAGIL+YM+LVD +AADF+  +M  N
Sbjct: 322 VTTPVGIAVGMAISSSYDDSSPTALIVQGVLNAASAGILIYMSLVDFLAADFMHPKMQSN 360

Query: 391 FRLQIVSYCTLFLGAGLMSLLALWA 412
            RLQI+++ +L +GAG+MSLLA WA
Sbjct: 382 TRLQIMAHISLLVGAGVMSLLAKWA 360

BLAST of CmoCh12G005790 vs. TAIR 10
Match: AT5G62160.1 (zinc transporter 12 precursor )

HSP 1 Score: 263.1 bits (671), Expect = 3.8e-70
Identity = 166/385 (43.12%), Postives = 226/385 (58.70%), Query Frame = 0

Query: 31  VSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGN 90
           VS +   + C  S+     + ++AL +K+IA  SIL +GV G+ +P+FG     LKT+ N
Sbjct: 23  VSAAEEENQCGGSKGGSAAEKASALKYKIIAFFSILIAGVFGVCLPIFG-----LKTESN 82

Query: 91  LFVATKAFAAGVILATAFVHMLPDGSKALSDPCL-PEFPWSKFPFSGFFAMMASLLTLLV 150
            F+  KAFAAGVILAT FVH+LPD +++L+  CL  E PW  FP +G  AM AS+LT+L+
Sbjct: 83  FFMYVKAFAAGVILATGFVHILPDATESLTSSCLGEEPPWGDFPMTGLVAMAASILTMLI 142

Query: 151 DFVGTQYYERKQGQIRLKEETNRVGSGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGM 210
           +   + Y  R     RL +             E   LP     V   GEE+    H    
Sbjct: 143 ESFASGYLNRS----RLAK-------------EGKTLP-----VSTGGEEEHA--HTGSA 202

Query: 211 HAHAAQHRHSHPQGKDACDGHVTLHDHGHGHSHG---LDGDDDESGVRHVVVSQILELGI 270
           H HA+Q                       GHSHG   +  DDD   +R  +V+QILELGI
Sbjct: 203 HTHASQ-----------------------GHSHGSLLIPQDDDHIDMRKKIVTQILELGI 262

Query: 271 VSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFA 330
           V HSVIIG+SLG S S  TI+PLIAA++FHQ FEGF LGGCIS+A+F      +M +FFA
Sbjct: 263 VVHSVIIGISLGASPSVSTIKPLIAAITFHQLFEGFGLGGCISEAKFRVKKIWVMLMFFA 322

Query: 331 ATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCN 390
            T P+GI IG  ++  YN NS  AL   G L++ ++GIL+YMALVDL+A  F++++   +
Sbjct: 323 LTAPIGIGIGIGVAEIYNENSPMALKVSGFLNATASGILIYMALVDLVAPLFMNQKTQSS 355

Query: 391 FRLQIVSYCTLFLGAGLMSLLALWA 412
            ++Q+    +L +GAGLMSLLA+WA
Sbjct: 383 MKIQVACSVSLVVGAGLMSLLAIWA 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LE594.3e-14364.08Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT... [more]
O040893.2e-13870.21Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 S... [more]
Q5Z6539.3e-11458.35Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2[more]
O826437.4e-11162.18Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1[more]
Q6L8F75.9e-10057.14Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GGQ18.5e-227100.00zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KKC01.4e-22499.03zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A6J1HFN18.5e-20389.13zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A0A0LKS64.2e-20288.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1[more]
A0A6J1K6U32.7e-20188.22zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114917... [more]
Match NameE-valueIdentityDescription
AT1G60960.13.0e-14464.08iron regulated transporter 3 [more]
AT1G10970.18.9e-14466.11zinc transporter 4 precursor [more]
AT4G33020.15.3e-11262.18ZIP metal ion transporter family [more]
AT1G05300.11.6e-7643.64zinc transporter 5 precursor [more]
AT5G62160.13.8e-7043.12zinc transporter 12 precursor [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003689Zinc/iron permeasePFAMPF02535Zipcoord: 56..408
e-value: 2.2E-77
score: 260.5
IPR004698Zinc/iron permease, fungal/plantTIGRFAMTIGR00820TIGR00820coord: 40..411
e-value: 1.9E-132
score: 439.3
NoneNo IPR availablePANTHERPTHR11040:SF157ZIP ZINC/IRON TRANSPORT FAMILY PROTEINcoord: 35..411
NoneNo IPR availablePANTHERPTHR11040ZINC/IRON TRANSPORTERcoord: 35..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G005790.1CmoCh12G005790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity