CmoCh12G005710 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G005710
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionFerrochelatase
LocationCmo_Chr12: 3520853 .. 3523881 (+)
RNA-Seq ExpressionCmoCh12G005710
SyntenyCmoCh12G005710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGATGCGTGGATGCGTAGACTCTTCAACCCCAAACCCAAATTCAAAATGAAACTGGCAACTTCGCTACGAATCAACGCTTTCCCGGATCTTCCCGTCCCCCAATTTTGACTCTTCATCCTTCCAATTCATCATTCGCGTTTCAAAAAATATTTATAATCAGGATCGTAGCTAACTCACTCAATTCTTCGTTCAACACTGCTCAAATTTTTCTGGGGTTTTCGTGGACTTTCGATTTTGGATCACTGTATTTCTGATTTTTGACTTCATGGACGCGATCGCTTTCTCTGCCGCTCATTCCAATATGAAGCTTCTCGATTCCAACACCCTTAATCCGGATCGCACGGTTTCTACGTAAGTTCTTTAATCCATCGCTTCTGCTGCCCTAAAGATTTCGCTTGTGTTTGAGATTGATGATCGGCTTCTTGAGTTGTCTTTTGTTCTTACTTTGGAATCTAGAATTCTGTGAAAATTTTGATCCATGTGAACTTTATACTGCTGTGATGCAATGTTTGTGCGGCTTGAAGTTGTCTGGATTATAATAACAGAGTTGATCGTAGTGAACTTTTTTGCTTATTGTGTGGAAGATGGCAGCGCAATGGCGTAGATTCTTGAAGACGTTTTTAGGTATTATTATATCAAAGTTAAGAATTCAACGTATGATTTTTGGTTTTTCAGGTCATTCTCTGTGCCGAAATCTCTTGTGTCTTTCTCTTGTAAATCCTCTGGAAAATTGCAAGTTCGTGATAAGTCTAGAACTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCAATGGTCAGGGGCTACAACTTTCTGGCCCTGTTGAGAAGAGAGGCCGTCTTGGACAAGCATTTTGCTCAGTAGGAACTGTCTCCTATGGTGAATTTGCTTTAGAATCTCACTCTCAGGCAGTAGAAGAGAAGGTTGGAGTTCTGCTCCTGAATCTCGGAGGGCCAGAAACGCTTGATGATGTTCAGCCATTTTTGTACAACCTATTTGCTGATCCGGTATCTTACTTAATCTTGTGTTTCTAGCCTTAATAACATGCCAATTTATATCTGTTTTTCAATTCTTTTCATAATACATAGGATATTATTCGGCTCCCGAGGTTGTTCCGTTTTCTCCAAGAGCCATTGGCGAAGTTAATTTCTACTTTTCGTGCTCCGAAAAGCAAAGAAGGGTATGCTTCAATAGGTGGTGGCTCACCTTTGCGTAAAATAACAGATGAACAGGTTGATTGACCTCGCTATTACTTCCCTCTACTATAAAAAACATTCAAATATTTATAATTTGTGAATCCTTGTTCTAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACATATCTGCTAATGTCTATGTCGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCGATTCAGCAAGTTCGTTTACATGTTCTTCTTATTCCCTGTGCCTTCTAGTTCGATACTGACCACGTGTATTGTCTTTCTTAATCATCACTGCATTTAAGTTAACAATTGATGGTACCTTTTGCTGTTTGTGGTAGATTAAGAGGGATGGGATAACAAAACTTGTGGTGCTGCCACTGTATCCACAGTACTCTATTTCAACAACCGGATCCAGTATTCGTGTTCTCCAGAAGATTTTCAGGTGATGATCTAGTCCACTGTTGATCTTGTTATTAAAAGCTTCAGCTACTGACTTATTGACTATGAGCCTATCAGGGAAGACGCATATCTCTCGACATTGCCTGTTTCGATTATAAAGTCGTGGTATCAACGAGAAGGGTATATTAAGTCAATGGCTGACTTGATTCAGACAGAGCTGAAGAATTTTGTTAATCCTCAGGAGGTATTTGATTTTTGCTGAAACTGATTTTAGAGAGATGATTTAGTTTATGCAGCAAATGGCTGTTGTTGTCCAAGTCGATTAGTTCTTAATGACGGGTCATTTGCATTTGCATCCTTAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCTTACAAAGATGAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGTTGAAGGCCAGAGGGGTTGGAAATGAGCATACTCTTGCTTATCAGGTTAGTAGTAAATTTGGAGCCTGGCTTGGTTTCTTATCTTTTTCTTGTTTTCTGGATTTTCATTTTTGTTTATGTATTACATGTTCAGAGCCGCGTTGGACCTGTACAATGGCTAAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATCAAGAGCCTCCTAGCCGTTCCCGTGAGGTATGTTCTGTTCACAAAACATATGCAACTCATGTTCTAGGGACCTATAGAGTGAATATTGGTGGGACTGAATCATGTAACAAGTAAAATAAAATGCTCGGTGAAATTCGATCTTTCTCGTTTTCGTACATTGTTTTTATCTATTCTTTGTTAACTTAAGCGTGTGGCGTTTTATTTATAGTAAGTTTACTTGTTTTTTCCTAGCTTTGTGAGTGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCCGGGATCGAAAACTGGGGGCGTGTGCCCGCTCTCAACTGCAAGTCTTCATTCATTATGGATCTGGCTGATGCAGTAGTCGAAGCCCTTCCATCTGCTACGGCATTGTCTCCCCACGCAAGCTCCGCAGATACAAGTGATCATGGTTCTTTCCGATTTGATCCTATCAGATTGTTATTTGGTTCGATCCTTGCATTCATCTTGTTACTCTCACCCAAAGCGTTTCTAGCGTTTAGGAATAATTTCTATTTCTAATTCCATTTGACGATACGAAACATAGGTAGAATAGATCTGCATGGATTGGATTGGGGAGGGGATTGTTTTAAATTTACTTGAGATAAAAGGCAAAAATTTTCAGCATCGCAATCATAGTCATTACGTTTTGTACTAATTTTGTATTAGGGCAAACACTGATGGATTCAAGACAAATACCATTACACGATGAAAATAAAGGAGGAA

mRNA sequence

GCGATGCGTGGATGCGTAGACTCTTCAACCCCAAACCCAAATTCAAAATGAAACTGGCAACTTCGCTACGAATCAACGCTTTCCCGGATCTTCCCGTCCCCCAATTTTGACTCTTCATCCTTCCAATTCATCATTCGCGTTTCAAAAAATATTTATAATCAGGATCGTAGCTAACTCACTCAATTCTTCGTTCAACACTGCTCAAATTTTTCTGGGGTTTTCGTGGACTTTCGATTTTGGATCACTGTATTTCTGATTTTTGACTTCATGGACGCGATCGCTTTCTCTGCCGCTCATTCCAATATGAAGCTTCTCGATTCCAACACCCTTAATCCGGATCGCACGGTTTCTACGTCATTCTCTGTGCCGAAATCTCTTGTGTCTTTCTCTTGTAAATCCTCTGGAAAATTGCAAGTTCGTGATAAGTCTAGAACTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCAATGGTCAGGGGCTACAACTTTCTGGCCCTGTTGAGAAGAGAGGCCGTCTTGGACAAGCATTTTGCTCAGTAGGAACTGTCTCCTATGGTGAATTTGCTTTAGAATCTCACTCTCAGGCAGTAGAAGAGAAGGTTGGAGTTCTGCTCCTGAATCTCGGAGGGCCAGAAACGCTTGATGATGTTCAGCCATTTTTGTACAACCTATTTGCTGATCCGGATATTATTCGGCTCCCGAGGTTGTTCCGTTTTCTCCAAGAGCCATTGGCGAAGTTAATTTCTACTTTTCGTGCTCCGAAAAGCAAAGAAGGGTATGCTTCAATAGGTGGTGGCTCACCTTTGCGTAAAATAACAGATGAACAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACATATCTGCTAATGTCTATGTCGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCGATTCAGCAAATTAAGAGGGATGGGATAACAAAACTTGTGGTGCTGCCACTGTATCCACAGTACTCTATTTCAACAACCGGATCCAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGACGCATATCTCTCGACATTGCCTGTTTCGATTATAAAGTCGTGGTATCAACGAGAAGGGTATATTAAGTCAATGGCTGACTTGATTCAGACAGAGCTGAAGAATTTTGTTAATCCTCAGGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCTTACAAAGATGAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGTTGAAGGCCAGAGGGGTTGGAAATGAGCATACTCTTGCTTATCAGAGCCGCGTTGGACCTGTACAATGGCTAAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATCAAGAGCCTCCTAGCCGTTCCCGTGAGCTTTGTGAGTGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCCGGGATCGAAAACTGGGGGCGTGTGCCCGCTCTCAACTGCAAGTCTTCATTCATTATGGATCTGGCTGATGCAGTAGTCGAAGCCCTTCCATCTGCTACGGCATTGTCTCCCCACGCAAGCTCCGCAGATACAAGTGATCATGGTTCTTTCCGATTTGATCCTATCAGATTGTTATTTGGTTCGATCCTTGCATTCATCTTGTTACTCTCACCCAAAGCGTTTCTAGCGTTTAGGAATAATTTCTATTTCTAATTCCATTTGACGATACGAAACATAGGTAGAATAGATCTGCATGGATTGGATTGGGGAGGGGATTGTTTTAAATTTACTTGAGATAAAAGGCAAAAATTTTCAGCATCGCAATCATAGTCATTACGTTTTGTACTAATTTTGTATTAGGGCAAACACTGATGGATTCAAGACAAATACCATTACACGATGAAAATAAAGGAGGAA

Coding sequence (CDS)

ATGGACGCGATCGCTTTCTCTGCCGCTCATTCCAATATGAAGCTTCTCGATTCCAACACCCTTAATCCGGATCGCACGGTTTCTACGTCATTCTCTGTGCCGAAATCTCTTGTGTCTTTCTCTTGTAAATCCTCTGGAAAATTGCAAGTTCGTGATAAGTCTAGAACTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCAATGGTCAGGGGCTACAACTTTCTGGCCCTGTTGAGAAGAGAGGCCGTCTTGGACAAGCATTTTGCTCAGTAGGAACTGTCTCCTATGGTGAATTTGCTTTAGAATCTCACTCTCAGGCAGTAGAAGAGAAGGTTGGAGTTCTGCTCCTGAATCTCGGAGGGCCAGAAACGCTTGATGATGTTCAGCCATTTTTGTACAACCTATTTGCTGATCCGGATATTATTCGGCTCCCGAGGTTGTTCCGTTTTCTCCAAGAGCCATTGGCGAAGTTAATTTCTACTTTTCGTGCTCCGAAAAGCAAAGAAGGGTATGCTTCAATAGGTGGTGGCTCACCTTTGCGTAAAATAACAGATGAACAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACATATCTGCTAATGTCTATGTCGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCGATTCAGCAAATTAAGAGGGATGGGATAACAAAACTTGTGGTGCTGCCACTGTATCCACAGTACTCTATTTCAACAACCGGATCCAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGACGCATATCTCTCGACATTGCCTGTTTCGATTATAAAGTCGTGGTATCAACGAGAAGGGTATATTAAGTCAATGGCTGACTTGATTCAGACAGAGCTGAAGAATTTTGTTAATCCTCAGGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCTTACAAAGATGAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGTTGAAGGCCAGAGGGGTTGGAAATGAGCATACTCTTGCTTATCAGAGCCGCGTTGGACCTGTACAATGGCTAAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATCAAGAGCCTCCTAGCCGTTCCCGTGAGCTTTGTGAGTGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCCGGGATCGAAAACTGGGGGCGTGTGCCCGCTCTCAACTGCAAGTCTTCATTCATTATGGATCTGGCTGATGCAGTAGTCGAAGCCCTTCCATCTGCTACGGCATTGTCTCCCCACGCAAGCTCCGCAGATACAAGTGATCATGGTTCTTTCCGATTTGATCCTATCAGATTGTTATTTGGTTCGATCCTTGCATTCATCTTGTTACTCTCACCCAAAGCGTTTCTAGCGTTTAGGAATAATTTCTATTTCTAA

Protein sequence

MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLVVSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFLAFRNNFYF
Homology
BLAST of CmoCh12G005710 vs. ExPASy Swiss-Prot
Match: P42044 (Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 1.9e-235
Identity = 419/492 (85.16%), Postives = 448/492 (91.06%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDS-NTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGL 60
           MDA + S A SN+KL  S NTLN D+ +S+  S+PKS V+FSCK+SG LQVRD  R+ GL
Sbjct: 1   MDAASSSLALSNIKLHGSTNTLNSDQRISSLCSLPKSRVTFSCKTSGNLQVRD--RSTGL 60

Query: 61  VVSCSSSNG-----QGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVL 120
           VVSCSSSNG     QGL LSGP+EK+ RLGQA CSVGT + GEFALES SQAV++KVGVL
Sbjct: 61  VVSCSSSNGDRDVIQGLHLSGPIEKKSRLGQACCSVGTFTVGEFALESQSQAVDDKVGVL 120

Query: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIG 180
           LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLIST+RAPKSKEGYASIG
Sbjct: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTYRAPKSKEGYASIG 180

Query: 181 GGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPL 240
           GGSPLRKITDEQAQALKMAL EKN+S NVYVGMRYWYPFTEEAIQQIKRDGIT+LVVLPL
Sbjct: 181 GGSPLRKITDEQAQALKMALAEKNMSTNVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240

Query: 241 YPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVN 300
           YPQYSISTTGSSIRVLQK+FREDAYLS+LPVSIIKSWYQREGYIKSMADL+Q ELKNF N
Sbjct: 241 YPQYSISTTGSSIRVLQKMFREDAYLSSLPVSIIKSWYQREGYIKSMADLMQAELKNFAN 300

Query: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPV 360
           PQEVMIFFSAHGVPVSYVENAGDPYKD+MEECI LIMQELKARG+GNEHTLAYQSRVGPV
Sbjct: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECICLIMQELKARGIGNEHTLAYQSRVGPV 360

Query: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420
           QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGI+NWGRVP
Sbjct: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIQNWGRVP 420

Query: 421 ALNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLL 480
           ALNC SSFI DLADAV+EALPSATAL+PH SS D  DH  F +  I+LLFGS+LAFILLL
Sbjct: 421 ALNCNSSFISDLADAVIEALPSATALAPHTSSTDADDHDPFLY-AIKLLFGSVLAFILLL 480

Query: 481 SPKAFLAFRNNF 487
           SPKAF+ FRNNF
Sbjct: 481 SPKAFMVFRNNF 489

BLAST of CmoCh12G005710 vs. ExPASy Swiss-Prot
Match: Q69TB1 (Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0297000 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.0e-172
Identity = 303/381 (79.53%), Postives = 337/381 (88.45%), Query Frame = 0

Query: 104 SQAVEEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRA 163
           S A EEKVGVLLLNLGGPETLDDVQPFL+NLFADPDIIRLPRLFRFLQ PLAKLISTFRA
Sbjct: 100 SHAAEEKVGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRA 159

Query: 164 PKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKR 223
           PKSKEGYASIGGGSPLRKITDEQA ALK+AL++KN++AN+YVGMRYWYPFTEEAI QIK+
Sbjct: 160 PKSKEGYASIGGGSPLRKITDEQANALKVALKKKNLNANIYVGMRYWYPFTEEAIDQIKK 219

Query: 224 DGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMAD 283
           D ITKLVVLPLYPQYSIST+GSSIRVLQ I +ED+Y + LP+SII+SWYQR+GY+KSMAD
Sbjct: 220 DKITKLVVLPLYPQYSISTSGSSIRVLQNIVKEDSYFAGLPISIIESWYQRDGYVKSMAD 279

Query: 284 LIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEH 343
           LI+ EL  F NP+EVMIFFSAHGVP++YV +AGDPY+D+ME+CI LIM ELK+RG+ N H
Sbjct: 280 LIEKELSIFSNPEEVMIFFSAHGVPLTYVTDAGDPYRDQMEDCIALIMGELKSRGILNSH 339

Query: 344 TLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLAL 403
           TLAYQSRVGPVQWLKPYTDEVLVELGQ+G+KSLLAVPVSFVSEHIETLEEIDMEYK LAL
Sbjct: 340 TLAYQSRVGPVQWLKPYTDEVLVELGQQGVKSLLAVPVSFVSEHIETLEEIDMEYKELAL 399

Query: 404 ESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLL 463
           ESGIENWGRVPAL C SSFI DLADAVVEALPSA+AL         SD     +   ++ 
Sbjct: 400 ESGIENWGRVPALGCTSSFISDLADAVVEALPSASALVTKKVDESDSDMDLMHY-LSKMF 459

Query: 464 FGSILAFILLLSPKAFLAFRN 485
           FGSILAF+LLLSP+   AFRN
Sbjct: 460 FGSILAFVLLLSPRLISAFRN 479

BLAST of CmoCh12G005710 vs. ExPASy Swiss-Prot
Match: P42045 (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.2e-170
Identity = 299/381 (78.48%), Postives = 335/381 (87.93%), Query Frame = 0

Query: 104 SQAVEEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRA 163
           S AVEEKVGVLLLNLGGPETL+DVQPFL+NLFADPDIIRLPRLFRFLQ PLAKLISTFRA
Sbjct: 102 SHAVEEKVGVLLLNLGGPETLNDVQPFLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRA 161

Query: 164 PKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKR 223
           PKS EGYASIGGGSPLRKITDEQA ALK+AL+ KN+ A++YVGMRYWYPFTEEAI QIK+
Sbjct: 162 PKSNEGYASIGGGSPLRKITDEQANALKVALKSKNLEADIYVGMRYWYPFTEEAIDQIKK 221

Query: 224 DGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMAD 283
           D ITKLVVLPLYPQYSIST+GSSIRVLQ I +ED Y + LP+SII+SWYQREGY+KSMAD
Sbjct: 222 DKITKLVVLPLYPQYSISTSGSSIRVLQNIVKEDPYFAGLPISIIESWYQREGYVKSMAD 281

Query: 284 LIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEH 343
           LI+ EL  F NP+EVMIFFSAHGVP++YV++AGDPY+D+ME+CI LIM+ELK+RG  N+H
Sbjct: 282 LIEKELSVFSNPEEVMIFFSAHGVPLTYVKDAGDPYRDQMEDCIALIMEELKSRGTLNDH 341

Query: 344 TLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLAL 403
           TLAYQSRVGPVQWLKPYTDEVLVELGQKG+KSLLAVPVSFVSEHIETLEEIDMEY+ LAL
Sbjct: 342 TLAYQSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEEIDMEYRELAL 401

Query: 404 ESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLL 463
           ESGIENWGRVPAL C SSFI DLADAVVEALPSA+A++        SD     +   ++ 
Sbjct: 402 ESGIENWGRVPALGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMDMMHY-LTKMF 461

Query: 464 FGSILAFILLLSPKAFLAFRN 485
            GS+LAF LLLSP+   AFRN
Sbjct: 462 LGSVLAFFLLLSPRLVSAFRN 481

BLAST of CmoCh12G005710 vs. ExPASy Swiss-Prot
Match: P42043 (Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FC1 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 2.7e-168
Identity = 301/404 (74.50%), Postives = 347/404 (85.89%), Query Frame = 0

Query: 85  QAFCSVGTVSYGEFALESHSQAV-EEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRL 144
           QA    G  SY E + ++ S  V E+K+GVLLLNLGGPETL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 145 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANV 204
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KNI+ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 205 YVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTL 264
           YVGMRYWYPFTEEA+QQIK+D IT+LVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 265 PVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEM 324
           PV+IIKSWYQR GY+ SMADLI+ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ +M
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 325 EECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 384
           EECI LIM+ELKARGV N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 385 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS-P 444
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAV+E+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 445 HA--SSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFLAFRN 485
           +A   S D+    +F +  +++ FGSILAF+LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSY-IVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmoCh12G005710 vs. ExPASy Swiss-Prot
Match: Q0DIV0 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0361200 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.6e-149
Identity = 273/408 (66.91%), Postives = 323/408 (79.17%), Query Frame = 0

Query: 54  SRTQGLVVSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESH-------SQA 113
           SRT   + SCS S+  GL L     K+       C +G  S    +L  H       S+A
Sbjct: 40  SRTYA-IKSCSVSSRTGLCLGQCYHKKS--SACKCKLGWSSQPLSSLRHHLRVHSSASEA 99

Query: 114 V-------------EEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEP 173
           V              EK+GVLLLNLGGPETLDDVQPFL+NLFADPDIIRLPRLFRFLQ+P
Sbjct: 100 VLTSQSDFTKLLVGNEKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKP 159

Query: 174 LAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPF 233
           LA+ IS  RAPKSKEGYASIGGGSPLR+ITD QA+AL+ AL +K+I A VYVGMRYW+PF
Sbjct: 160 LAQFISVVRAPKSKEGYASIGGGSPLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPF 219

Query: 234 TEEAIQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQ 293
           TEEAI+QIKRDGITKLVVLPLYPQ+SIST+GSS+R+L+ IFRED YL  +  ++I SWYQ
Sbjct: 220 TEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQ 279

Query: 294 REGYIKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQE 353
           REGYIK+MA LI+ EL+ F  PQ+VMIFFSAHGVP++YVE AGDPYK EMEEC+ LIM+E
Sbjct: 280 REGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEE 339

Query: 354 LKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEE 413
           L+ RG+ N  TLAYQSRVGPV+WL+PYTDE ++ELGQKG+KSLLAVP+SFVSEHIETLEE
Sbjct: 340 LEKRGITNSCTLAYQSRVGPVEWLRPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEE 399

Query: 414 IDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS 442
           ID+EYK LALESGI++WGRVPAL C+ +FI DLADAV+E+LP   A++
Sbjct: 400 IDVEYKELALESGIKHWGRVPALGCEPTFITDLADAVIESLPYVGAMA 444

BLAST of CmoCh12G005710 vs. ExPASy TrEMBL
Match: A0A6J1GJ53 (Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111454682 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.4e-276
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV 60
           MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV
Sbjct: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV 60

Query: 61  VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG 120
           VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG
Sbjct: 61  VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG 120

Query: 121 PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLR 180
           PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLR
Sbjct: 121 PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLR 180

Query: 181 KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI 240
           KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI
Sbjct: 181 KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI 240

Query: 241 STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMI 300
           STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMI
Sbjct: 241 STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMI 300

Query: 301 FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY 360
           FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY
Sbjct: 301 FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY 360

Query: 361 TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS 420
           TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS
Sbjct: 361 TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS 420

Query: 421 SFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFL 480
           SFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFL
Sbjct: 421 SFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFL 480

Query: 481 AFRNNFYF 489
           AFRNNFYF
Sbjct: 481 AFRNNFYF 488

BLAST of CmoCh12G005710 vs. ExPASy TrEMBL
Match: A0A6J1KUQ2 (Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111496575 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.1e-271
Identity = 480/486 (98.77%), Postives = 483/486 (99.38%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV 60
           MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKS VSFSCKSSGKLQVRDKSRTQGLV
Sbjct: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSHVSFSCKSSGKLQVRDKSRTQGLV 60

Query: 61  VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG 120
           VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG
Sbjct: 61  VSCSSSNGQGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLLLNLGG 120

Query: 121 PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLR 180
           PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRA KSKEGYASIGGGSPLR
Sbjct: 121 PETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRALKSKEGYASIGGGSPLR 180

Query: 181 KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI 240
           KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI
Sbjct: 181 KITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSI 240

Query: 241 STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMI 300
           STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQ ELKNFVNPQEVMI
Sbjct: 241 STTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQAELKNFVNPQEVMI 300

Query: 301 FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY 360
           FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY
Sbjct: 301 FFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPY 360

Query: 361 TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS 420
           TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS
Sbjct: 361 TDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKS 420

Query: 421 SFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFL 480
           SFI+DLADAVVEALPSATALSPHASSADT++HGSFRFDPIRLLFGSILAFILLLSPKAFL
Sbjct: 421 SFIVDLADAVVEALPSATALSPHASSADTNNHGSFRFDPIRLLFGSILAFILLLSPKAFL 480

Query: 481 AFRNNF 487
           AFRNNF
Sbjct: 481 AFRNNF 486

BLAST of CmoCh12G005710 vs. ExPASy TrEMBL
Match: A0A6J1HEW3 (Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.9e-239
Identity = 427/491 (86.97%), Postives = 449/491 (91.45%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV 60
           MDA + S A SNMKLL +N LN D  V T FS+PK LVS SCKS   LQVRDKS +QGLV
Sbjct: 1   MDATSSSGALSNMKLLSANKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNG-----QGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLL 120
           VSCSSSN      QG  LSGP+EKR RLGQAFCSV + +YGE+ALES SQA EEKVGVLL
Sbjct: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGP+TLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKN SANVYVGMRYWYPFTEEAIQQIKRDGIT+LVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNP 300
           PQYSISTTGSSIRVLQKIFREDA+LS LPVS+IKSWYQREGYIKSMADL+Q ELKNF+NP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAHLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKD+MEECIYLIMQELKARG+GNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLS 480
           LNCKSSFI+DLADAV+EALPSATALSPHASS D  D   F++  I+LLFGS+LA ILLLS
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQY-AIKLLFGSVLAIILLLS 480

Query: 481 PKAFLAFRNNF 487
           PKAFLAFRNNF
Sbjct: 481 PKAFLAFRNNF 490

BLAST of CmoCh12G005710 vs. ExPASy TrEMBL
Match: A0A6J1K7N7 (Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 6.7e-239
Identity = 428/493 (86.82%), Postives = 450/493 (91.28%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDSNTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGLV 60
           MDA + S A SN+KLL SN LN D  V T FS+PK LVS SCKS   LQVRDKS +QGLV
Sbjct: 1   MDATSSSGALSNVKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNG-----QGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVLL 120
           VSCSSS       QGL LSGP+EKR RLGQAFCSVG+ + GE+ALES SQA EEKVGVLL
Sbjct: 61  VSCSSSISHRDVMQGLHLSGPIEKRSRLGQAFCSVGSFTCGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGP+TLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKN SANVYVGMRYWYPFTEEAIQQIKRDGIT+LVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVNP 300
           PQYSISTTGSSIRVLQKIFREDAYLS LPVS+IKSWYQREGYIKSMADL+Q ELKNF+NP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKD+MEECIYLIMQELKARG+GNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLLS 480
           LNCKSSFI+DLADAV+EALPSATALSPHASS D  D   F++  I+LLFGS+LA ILLLS
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQY-AIKLLFGSVLAIILLLS 480

Query: 481 PKAFLAFRNNFYF 489
           PKAFLAFRNNF +
Sbjct: 481 PKAFLAFRNNFIY 492

BLAST of CmoCh12G005710 vs. ExPASy TrEMBL
Match: A0A5A7V5D0 (Ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001260 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.7e-234
Identity = 422/492 (85.77%), Postives = 450/492 (91.46%), Query Frame = 0

Query: 1   MDAIAFSAAHSNMKLLDS-NTLNPDRTVSTSFSVPKSLVSFSCKSSGKLQVRDKSRTQGL 60
           MDA + S A SN+KL  S NTLN D+ +S+  S+PKS V+FSCKSSG LQV D  R+ GL
Sbjct: 1   MDAASSSLALSNIKLHGSTNTLNSDKRISSLCSLPKSGVAFSCKSSGNLQVHD--RSTGL 60

Query: 61  VVSCSSSNG-----QGLQLSGPVEKRGRLGQAFCSVGTVSYGEFALESHSQAVEEKVGVL 120
           VVSCSSSNG     QGL LSGP+EK+ RLGQA CSVGT + GEFALES SQAV++KVGVL
Sbjct: 61  VVSCSSSNGHRDVIQGLHLSGPIEKKSRLGQACCSVGTFTVGEFALESQSQAVDDKVGVL 120

Query: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIG 180
           LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLIST+RAPKSKEGYASIG
Sbjct: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTYRAPKSKEGYASIG 180

Query: 181 GGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEAIQQIKRDGITKLVVLPL 240
           GGSPLRKITDEQAQALKMALEEKN+SANVYVGMRYWYPFTEEAI+QIKRDGIT+LVVLPL
Sbjct: 181 GGSPLRKITDEQAQALKMALEEKNVSANVYVGMRYWYPFTEEAIEQIKRDGITRLVVLPL 240

Query: 241 YPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGYIKSMADLIQTELKNFVN 300
           YPQYSISTTGSSIRVLQK+FREDAYLS+LPVSIIKSWYQREGYI+SMADLIQ ELKNF N
Sbjct: 241 YPQYSISTTGSSIRVLQKMFREDAYLSSLPVSIIKSWYQREGYIRSMADLIQAELKNFAN 300

Query: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGVGNEHTLAYQSRVGPV 360
           P+EVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARG+GNEHTLAYQSRVGPV
Sbjct: 301 PREVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGIGNEHTLAYQSRVGPV 360

Query: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420
           QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP
Sbjct: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420

Query: 421 ALNCKSSFIMDLADAVVEALPSATALSPHASSADTSDHGSFRFDPIRLLFGSILAFILLL 480
           ALNC S+FI DLADAV+EALPSATALSPH SS D  D    R+  I+LLFGS+LAFILLL
Sbjct: 421 ALNCNSTFISDLADAVIEALPSATALSPHTSSTDADDRDPLRY-AIKLLFGSVLAFILLL 480

Query: 481 SPKAFLAFRNNF 487
           SPKAF+AFRNNF
Sbjct: 481 SPKAFMAFRNNF 489

BLAST of CmoCh12G005710 vs. TAIR 10
Match: AT5G26030.1 (ferrochelatase 1 )

HSP 1 Score: 593.2 bits (1528), Expect = 1.9e-169
Identity = 301/404 (74.50%), Postives = 347/404 (85.89%), Query Frame = 0

Query: 85  QAFCSVGTVSYGEFALESHSQAV-EEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRL 144
           QA    G  SY E + ++ S  V E+K+GVLLLNLGGPETL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 145 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANV 204
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KNI+ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 205 YVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTL 264
           YVGMRYWYPFTEEA+QQIK+D IT+LVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 265 PVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEM 324
           PV+IIKSWYQR GY+ SMADLI+ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ +M
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 325 EECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 384
           EECI LIM+ELKARGV N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 385 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS-P 444
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAV+E+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 445 HA--SSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFLAFRN 485
           +A   S D+    +F +  +++ FGSILAF+LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSY-IVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmoCh12G005710 vs. TAIR 10
Match: AT5G26030.2 (ferrochelatase 1 )

HSP 1 Score: 593.2 bits (1528), Expect = 1.9e-169
Identity = 301/404 (74.50%), Postives = 347/404 (85.89%), Query Frame = 0

Query: 85  QAFCSVGTVSYGEFALESHSQAV-EEKVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRL 144
           QA    G  SY E + ++ S  V E+K+GVLLLNLGGPETL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 145 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANV 204
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KNI+ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 205 YVGMRYWYPFTEEAIQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTL 264
           YVGMRYWYPFTEEA+QQIK+D IT+LVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 265 PVSIIKSWYQREGYIKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEM 324
           PV+IIKSWYQR GY+ SMADLI+ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ +M
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 325 EECIYLIMQELKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 384
           EECI LIM+ELKARGV N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 385 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS-P 444
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAV+E+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 445 HA--SSADTSDHGSFRFDPIRLLFGSILAFILLLSPKAFLAFRN 485
           +A   S D+    +F +  +++ FGSILAF+LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSY-IVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmoCh12G005710 vs. TAIR 10
Match: AT2G30390.1 (ferrochelatase 2 )

HSP 1 Score: 508.8 bits (1309), Expect = 4.7e-144
Identity = 247/344 (71.80%), Postives = 297/344 (86.34%), Query Frame = 0

Query: 99  ALESHSQAVEE-KVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 158
           A+ S S   ++ K+GVLLLNLGGPETLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 159 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEA 218
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN+ A VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 219 IQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGY 278
           I+QIKRDGITKLVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  ++I SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 279 IKSMADLIQTELKNFVNPQEVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKAR 338
           IK+MA+LIQ+EL  F +P +V+IFFSAHGVP++YVE AGDPYK EMEEC+ LIM+EL  R
Sbjct: 268 IKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELDKR 327

Query: 339 GVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDME 398
            + N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEHIETLEEID+E
Sbjct: 328 KITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEHIETLEEIDVE 387

Query: 399 YKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS 442
           YK LAL+SGI+NWGRVPAL  +  FI DLADAVVE+LP   A++
Sbjct: 388 YKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 431

BLAST of CmoCh12G005710 vs. TAIR 10
Match: AT2G30390.2 (ferrochelatase 2 )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 247/354 (69.77%), Postives = 297/354 (83.90%), Query Frame = 0

Query: 99  ALESHSQAVEE-KVGVLLLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 158
           A+ S S   ++ K+GVLLLNLGGPETLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 159 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNISANVYVGMRYWYPFTEEA 218
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN+ A VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 219 IQQIKRDGITKLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSTLPVSIIKSWYQREGY 278
           I+QIKRDGITKLVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  ++I SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 279 IKSMADLIQTELKNFVNPQ----------EVMIFFSAHGVPVSYVENAGDPYKDEMEECI 338
           IK+MA+LIQ+EL  F +P           +V+IFFSAHGVP++YVE AGDPYK EMEEC+
Sbjct: 268 IKAMANLIQSELGKFGSPNQTCFKPKYMLQVVIFFSAHGVPLAYVEEAGDPYKAEMEECV 327

Query: 339 YLIMQELKARGVGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEH 398
            LIM+EL  R + N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEH
Sbjct: 328 DLIMEELDKRKITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEH 387

Query: 399 IETLEEIDMEYKHLALESGIENWGRVPALNCKSSFIMDLADAVVEALPSATALS 442
           IETLEEID+EYK LAL+SGI+NWGRVPAL  +  FI DLADAVVE+LP   A++
Sbjct: 388 IETLEEIDVEYKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P420441.9e-23585.16Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1[more]
Q69TB14.0e-17279.53Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09... [more]
P420452.2e-17078.48Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1[more]
P420432.7e-16874.50Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q0DIV01.6e-14966.91Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05... [more]
Match NameE-valueIdentityDescription
A0A6J1GJ532.4e-276100.00Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111454682 PE=3 SV=1[more]
A0A6J1KUQ25.1e-27198.77Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111496575 PE=3 SV=1[more]
A0A6J1HEW33.9e-23986.97Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1[more]
A0A6J1K7N76.7e-23986.82Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1[more]
A0A5A7V5D01.7e-23485.77Ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00126... [more]
Match NameE-valueIdentityDescription
AT5G26030.11.9e-16974.50ferrochelatase 1 [more]
AT5G26030.21.9e-16974.50ferrochelatase 1 [more]
AT2G30390.14.7e-14471.80ferrochelatase 2 [more]
AT2G30390.21.3e-14169.77ferrochelatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.1400coord: 272..415
e-value: 1.2E-121
score: 408.0
NoneNo IPR availableGENE3D3.40.50.1400coord: 110..435
e-value: 1.2E-121
score: 408.0
NoneNo IPR availablePANTHERPTHR11108:SF4FERROCHELATASE-1, CHLOROPLASTIC/MITOCHONDRIALcoord: 49..486
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 109..441
IPR001015FerrochelataseTIGRFAMTIGR00109TIGR00109coord: 109..433
e-value: 1.0E-115
score: 384.6
IPR001015FerrochelatasePFAMPF00762Ferrochelatasecoord: 110..433
e-value: 2.1E-111
score: 372.0
IPR001015FerrochelatasePANTHERPTHR11108FERROCHELATASEcoord: 49..486
IPR001015FerrochelataseHAMAPMF_00323Ferrochelatasecoord: 109..434
score: 36.894043
IPR019772Ferrochelatase, active sitePROSITEPS00534FERROCHELATASEcoord: 300..319
IPR033644Ferrochelatase, C-terminalCDDcd00419Ferrochelatase_Ccoord: 281..417
e-value: 3.95719E-57
score: 183.885
IPR033659Ferrochelatase, N-terminalCDDcd03411Ferrochelatase_Ncoord: 111..273
e-value: 2.31681E-71
score: 221.675

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G005710.1CmoCh12G005710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006783 heme biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004325 ferrochelatase activity