CmoCh12G004020 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G004020
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	abscisic acid 8'-hydroxylase 1
Location	Cmo_Chr12: 2503860 .. 2507931 (+)
RNA-Seq Expression	CmoCh12G004020
Synteny	CmoCh12G004020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTTTTCGCTCTGCTTTTGGTGCTTTCCGGCCGCCGCTTTGGGCGGAGCTGCCCTCTCGATCCTCATTGTTTTCCTATTTTCTGTGATGAGTAGCGCACGGCGGCCGAGTTCTCATTCTCCTCCTCCTCCTCCTGGAAGCAGAGGATTGCCTTTGATTGGAGAGACGATCCAGTTCATGGCCGCCGTCAATAGCAGCAACGGCGTTTATGATTTCGTCAGAATCCGCTGCCTCCGGTAATCAAAATTCATATGTTTCTAGTTATATACTCTTCAATTTCTTTTAATTTTTGCTTAATAGTAGATGACAATAATGTGGTGGGTTTGAACAGATATGGAAGATGTTTCAAGACTCGGATATTTGGGGAGACGCATGTGTTCGTATCGAGCACCGAGTCGGCGAAGCAGATACTCAACGACAGCGGAGTCAACTACACCAAGAAGTACATAAGGTCCATTGCAGAGCTAGTCGGGCATCGGAGTTTGCTCTGCGCCTCGCATCTTGACCACAAGTTCTTACGCAGCCATCTCATCAATCTATTCTCTACCAGTTTTTTGGCTTCCTTTGTCACGCAGTTCGATCAGCAGATCGTTGAGGCGCTTCGAGGCTGGGAAAGTGGATCCACCATTGTCCTCCTCAACCAAGCCCTCAAGGTAAATTTACCTTCACTCCTACAATCACTTTCATTTTGAGGATTGGTGGGAGACATTAACTCATTTGAGAGAATGATCATGCCTTTACTATGAGTAATATAACTTCTCTTTTGGGGAGTCCAAAGCAAAGTCACGAGAGCTTATGTTTAAAGTGGACAATATCATGCCATTGTGGAGATCAGTGACTCTTAATATGCCCTTAATTTAGTTATGACAATAGAATCCTCAAGTATCAAATAAAGAAGTTGCTAGCCTTAAAGGTGTAGTCAAAATTGACTCAGGTGTCGAACAAAGGGTGTACTTTGTTTGAGGGCTCTAGAGAAAAGAGTCGAGTCTCGATTAAGGGAAAGTTGTTCGAGGACTACATAGACCTCAGAGGAGGCTCTACGGTGTACTTTGTTCGAGGGTAGGATTATTGGGAGGGAGTCCCACGTTGGCTAATGTAGGGAAGGATCATGGATTTATAAATAAGGAATACATCTCCATTGGTATGAGGCCTTTTAGAGAAGCTCAAAACAAAACCATAAAAGCTTATGCTTTTATGCTCGAACTGGACAATATTTTACCACTATGGAAATCTGAGATCTGTGATTACTAACATTTCCGTCTCTATTGTTTAACAAATTCAATTTTATTGATGAAGATAACATGCAAAGCAATGTGCAAAATGCTAATGAGCATACAAAACGACGACGAATTAGAGCTACTCCAAAAGGAGGTAGCTCATGTATGTGAAGCTATGCTTGCATTCCCATGGCGGTTTCCTGGGACAAGATTCCACAACGGCCTCAAGGTCTCCTCCACCATTTCTGCAACTCATTTTCTTCATTCCCTCCATTTTTTCCGTACTCAACTCTGTAATTTCAGGCAAGAAGAAGAATCATTAAAATACTCGAAAAGATGATACGAGAGCGACGAAGATCAGAAGCTTGGCATGACGATTTCTTGCAACGGTTGTTGACGGAGGAGGGCGACGATAGTAGAGGAGCGTTGTTGGATGTGGAGATCGGAGATAACATATTGACGATGTTGATTGCAGGACAAGATACGACAGCAAGTGCCATCACGTGGATGGTGAAGTTCTTGGACGAGAACCCTGATGTTCTTCAAAATTTGAAGGTACGTTGATTCGTACATTATCTCTATTTTAATCTTTAAACTTTTAAAATATCTACTTGAACAAAAACGAAATAGGATGAACAATTTGAGATATTGAACAAACAACGAGATCGTGATCGTGAGAGTTGTTTCCTTACGTTGGAAGATCTTGGCAACATGTGTTATGCTCCTAAGGTATTAATTTTTTTTCTTTTGGAAAAATTAATGTTGTCACAAATTTTCTTTTTAAATAATGTTATTATGAATTAATCAGGTAGTAAAAGAATCCTTGAGGTTAGCATCGGTGGTGCCATGGTTCCCAAGGCTAGTCCTCCAAGACTCTCAAATTCAAGGTAATTTCATATTTTCTATTCATATTCTCCCATGTTCTTTCTTTTTTAGATTTTGAATCAATGTTTTAAATTTCAAATTTGTTCGGTTTTAATTTTTAAACTTCTAAAACATTCCAGTAGTCTTAAAACGTTCAAAAGTATCCATTTTAATCCTTCATGTGTTTTCGTCTAACATTTGTTTATCCTCATCCACGCCTTTGAGAACATGTTTTGAAAATATGTATCAATCAAAGTACATGATTAGTCGAAATTTTATATCTTTGAATAAAATGTTAGAGATTTGAATATCTTCCTCGTTAAACTTAAAAAATATAGTAATTAGTTAAACTTAAAAAATAGTAATTAATTAAACTTAAAAATTAATTGTAAATTAGGAAGGTTGAAAGTCTAAAATAAAACATATTTGTTTTGTTTTGTTTTGTTTTTCATTTTTTTCATTTTTTTGGTTTTTTTTTTTAATTCAAATAAATGAAAGTTTTATAGATTAAGTGTGTTCATAGATTCCTTTAAATTAGAATTGAACTAGTCCAAATTTATTATAAATTTCAAAAAATACATGTTTTTATTTAATTTCTTTAAAATATATTTTTTTAACGCGATGAAGGGTAATTTTTAACCTACATATTGAAATTGAGTTCGTAACTTTTATTAAATGTTCGAAAATTTTATAATTAATTAGGTCGAGATAGATTTATTAGTTAATAATTAGGTTGGGTTAGATTTATTGGTATTTAATAAGAAATTACATTTTAAATTTAAAAAATTTGAACAATTGGGTTTGGATTTAAAAAATGTTTCAACCCCAACCCACGAACCCCATTGGGTGAATAATGAAAAAGTGGGTCTAAAAAAATAAAAATAAATAAAAAAAATGATTATTTTTAATATAAAGTATTAGTTAGATTAATGTTAGTAATAGATACATGATTAATTTGGGCAATGTTGATTGGAAATAGGTTACAAAGTCAACGAAGGTTGGAATGTCAACATTGATGTAAGGTCACTACACTCACATCCTTCTATCTACCATCACCCTCTCATCTTCAATCCTTCCAGATTTGATGTAAGAATATTATTCTCTCTTCTTCTTTTTTTTTTTTTTTTTTTTTAATTATACTGTAAATTTTAAAATATGTCAACGAAAATTTAAAATTTAAAAACTTATCAAATAATTTAAAATATATATATAAATATTGATTAGACATTTTATTCATACATTTTTTAAAATTAAAAAAAAACCAGATAGACACGAATCTAAATTTAGTTAATATATATATATATATATAAATGTTGGTGTAGGAAGAAGCAAAGCCCTATAGTTTTCTAGCGTTTGGAATGGGAGGGAGGCAATGTCTAGGGATGAACCTGGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGGTGACAGACCAATTATATATATATATTTTTTTTTTTCATTTTCCCTTTTTTCTTTTCATCTTTGAAAAAATAAATTTTAGAAATTTGGATGCCAAACACGTTAATGAAAATGCAATTTTTGTTTTTCTTCTAAACAAATACGATATTGAGATTTTTTACCGGAACGCGATTGGTTTTTAAGATCGTTTCTGGTTTTGGGCCTTTACTAGTTAGTCCCAATAAAGCCCCCAGATAGCGTATTTTTTTTAAACGAGTCCTCTGTAATGCAACATAGACTCCTTATTTTATTTAAAGGCCTTTTACTAGTTAGTCCCGAATAAAGCCCCCAGATAGCGTTTTTTTTTTTAAACGAGCCCTCTGTAATGCAACATAAAGACTTCTTATTTTATTTAAATTTCATAAACCTAAATTAAAACTAAATGATAAATATGATAACTAAAGCGAAATCCAGTAAAGTATTGTGCGACAAAACAAAGATTAATTAGATGACTTTGATCAACATACGGATTCTATTGTATCTGTATATGATACACAAAAGTAAGAGTAC

mRNA sequence

ATGCCCTTTTCGCTCTGCTTTTGGTGCTTTCCGGCCGCCGCTTTGGGCGGAGCTGCCCTCTCGATCCTCATTGTTTTCCTATTTTCTGTGATGAGTAGCGCACGGCGGCCGAGTTCTCATTCTCCTCCTCCTCCTCCTGGAAGCAGAGGATTGCCTTTGATTGGAGAGACGATCCAGTTCATGGCCGCCGTCAATAGCAGCAACGGCGTTTATGATTTCGTCAGAATCCGCTGCCTCCGATATGGAAGATGTTTCAAGACTCGGATATTTGGGGAGACGCATGTGTTCGTATCGAGCACCGAGTCGGCGAAGCAGATACTCAACGACAGCGGAGTCAACTACACCAAGAAGTACATAAGGTCCATTGCAGAGCTAGTCGGGCATCGGAGTTTGCTCTGCGCCTCGCATCTTGACCACAAGTTCTTACGCAGCCATCTCATCAATCTATTCTCTACCAGTTTTTTGGCTTCCTTTGTCACGCAGTTCGATCAGCAGATCGTTGAGGCGCTTCGAGGCTGGGAAACAATGTGCAAAATGCTAATGAGCATACAAAACGACGACGAATTAGAGCTACTCCAAAAGGAGGTAGCTCATGTATGTGAAGCTATGCTTGCATTCCCATGGCGGTTTCCTGGGACAAGATTCCACAACGGCCTCAAGGCAAGAAGAAGAATCATTAAAATACTCGAAAAGATGATACGAGAGCGACGAAGATCAGAAGCTTGGCATGACGATTTCTTGCAACGGTTGTTGACGGAGGAGGGCGACGATAGTAGAGGAGCGTTGTTGGATGTGGAGATCGGAGATAACATATTGACGATGTTGATTGCAGGACAAGATACGACAGCAAGTGCCATCACGTGGATGGTGAAGTTCTTGGACGAGAACCCTGATGTTCTTCAAAATTTGAAGGATGAACAATTTGAGATATTGAACAAACAACGAGATCGTGATCGTGAGAGTTGTTTCCTTACGTTGGAAGATCTTGGCAACATGTGTTATGCTCCTAAGGTAGTAAAAGAATCCTTGAGGTTAGCATCGGTGGTGCCATGGTTCCCAAGGCTAGTCCTCCAAGACTCTCAAATTCAAGGTTACAAAGTCAACGAAGGTTGGAATGTCAACATTGATGTAAGGTCACTACACTCACATCCTTCTATCTACCATCACCCTCTCATCTTCAATCCTTCCAGATTTGATGAAGAAGCAAAGCCCTATAGTTTTCTAGCGTTTGGAATGGGAGGGAGGCAATGTCTAGGGATGAACCTGGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGGTGACAGACCAATTATATATATATATTTTTTTTTTTCATTTTCCCTTTTTTCTTTTCATCTTTGAAAAAATAAATTTTAGAAATTTGGATGCCAAACACGTTAATGAAAATGCAATTTTTGTTTTTCTTCTAAACAAATACGATATTGAGATTTTTTACCGGAACGCGATTGGTTTTTAAGATCGTTTCTGGTTTTGGGCCTTTACTAGTTAGTCCCAATAAAGCCCCCAGATAGCGTATTTTTTTTAAACGAGTCCTCTGTAATGCAACATAGACTCCTTATTTTATTTAAAGGCCTTTTACTAGTTAGTCCCGAATAAAGCCCCCAGATAGCGTTTTTTTTTTTAAACGAGCCCTCTGTAATGCAACATAAAGACTTCTTATTTTATTTAAATTTCATAAACCTAAATTAAAACTAAATGATAAATATGATAACTAAAGCGAAATCCAGTAAAGTATTGTGCGACAAAACAAAGATTAATTAGATGACTTTGATCAACATACGGATTCTATTGTATCTGTATATGATACACAAAAGTAAGAGTAC

Coding sequence (CDS)

ATGCCCTTTTCGCTCTGCTTTTGGTGCTTTCCGGCCGCCGCTTTGGGCGGAGCTGCCCTCTCGATCCTCATTGTTTTCCTATTTTCTGTGATGAGTAGCGCACGGCGGCCGAGTTCTCATTCTCCTCCTCCTCCTCCTGGAAGCAGAGGATTGCCTTTGATTGGAGAGACGATCCAGTTCATGGCCGCCGTCAATAGCAGCAACGGCGTTTATGATTTCGTCAGAATCCGCTGCCTCCGATATGGAAGATGTTTCAAGACTCGGATATTTGGGGAGACGCATGTGTTCGTATCGAGCACCGAGTCGGCGAAGCAGATACTCAACGACAGCGGAGTCAACTACACCAAGAAGTACATAAGGTCCATTGCAGAGCTAGTCGGGCATCGGAGTTTGCTCTGCGCCTCGCATCTTGACCACAAGTTCTTACGCAGCCATCTCATCAATCTATTCTCTACCAGTTTTTTGGCTTCCTTTGTCACGCAGTTCGATCAGCAGATCGTTGAGGCGCTTCGAGGCTGGGAAACAATGTGCAAAATGCTAATGAGCATACAAAACGACGACGAATTAGAGCTACTCCAAAAGGAGGTAGCTCATGTATGTGAAGCTATGCTTGCATTCCCATGGCGGTTTCCTGGGACAAGATTCCACAACGGCCTCAAGGCAAGAAGAAGAATCATTAAAATACTCGAAAAGATGATACGAGAGCGACGAAGATCAGAAGCTTGGCATGACGATTTCTTGCAACGGTTGTTGACGGAGGAGGGCGACGATAGTAGAGGAGCGTTGTTGGATGTGGAGATCGGAGATAACATATTGACGATGTTGATTGCAGGACAAGATACGACAGCAAGTGCCATCACGTGGATGGTGAAGTTCTTGGACGAGAACCCTGATGTTCTTCAAAATTTGAAGGATGAACAATTTGAGATATTGAACAAACAACGAGATCGTGATCGTGAGAGTTGTTTCCTTACGTTGGAAGATCTTGGCAACATGTGTTATGCTCCTAAGGTAGTAAAAGAATCCTTGAGGTTAGCATCGGTGGTGCCATGGTTCCCAAGGCTAGTCCTCCAAGACTCTCAAATTCAAGGTTACAAAGTCAACGAAGGTTGGAATGTCAACATTGATGTAAGGTCACTACACTCACATCCTTCTATCTACCATCACCCTCTCATCTTCAATCCTTCCAGATTTGATGAAGAAGCAAAGCCCTATAGTTTTCTAGCGTTTGGAATGGGAGGGAGGCAATGTCTAGGGATGAACCTGGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGGTGA

Protein sequence

MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWETMCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR

Homology

[Close]

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 3.4e-53
Identity = 138/435 (31.72%), Postives = 216/435 (49.66%), Query Frame = 0


Query: 22  ILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRY 81
           IL + L  V+ S ++ +S     PPGS G P +GET+Q       S     F   +  RY
Sbjct: 13  ILCLLLVRVIVSKKKKNSRG-KLPPGSMGWPYLGETLQLY-----SQNPNVFFTSKQKRY 72

Query: 82  GRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKF 141
           G  FKTRI G   V ++S E+A+ +L      +   Y RS  +L+G  +L       H  
Sbjct: 73  GEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSH 132

Query: 142 LRSHLINLFSTSFLASFVTQFDQQIVEALRGWETM-----------------CKMLMSIQ 201
           +R  + + F    +   +   +   + +L+ W  M                    +    
Sbjct: 133 IRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHL 192

Query: 202 NDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHD 261
                E+L+     V +   +FP   PGT +H  L AR+++  I+ ++I ERR   A   
Sbjct: 193 ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQT 252

Query: 262 DFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLK 321
           DFL  LL  + +  R  L   +I DNI+ +L A QDTTAS +TW++K+L ++  +L+ +K
Sbjct: 253 DFLGHLLNFKNEKGR-VLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVK 312

Query: 322 DEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQG 381
            EQ  I     +  RE   LT     NM    KV+ ESLR+AS++ +  R  + D + +G
Sbjct: 313 AEQKAI---YEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKG 372

Query: 382 YKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAK 440
           Y + +GW V    R++H +P  + +P +F+PSRF+   KP +F+ FG G   C G  LAK
Sbjct: 373 YLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAK 432

[Close]

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.2e-52
Identity = 136/434 (31.34%), Postives = 223/434 (51.38%), Query Frame = 0


Query: 23  LIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYG 82
           L ++    + S RR  S   P PPG+ G P +GET Q       S     F + +  RYG
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRYG 74

Query: 83  RCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFL 142
             FKT + G   V +SS E+AK +L      +   +  S   ++G +++       H  L
Sbjct: 75  SVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKL 134

Query: 143 RSHLINLFSTSFLASFVTQFDQQIVEALRGWE-------------TMCKMLMSIQNDDEL 202
           R  ++  F    + + V   +    ++LR WE             T    L+SI   D  
Sbjct: 135 RKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKD-- 194

Query: 203 ELLQKEVAHVCEAML-----AFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHD 262
           E+L +E    C  +L     + P   PGT FH  +KAR+ + +IL +++ ERR++ + H+
Sbjct: 195 EVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHN 254

Query: 263 DFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLK 322
           D L   + ++ +     L D +I DNI+ ++ A +DTTAS ++W++K+L ENP+VL+ + 
Sbjct: 255 DLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVT 314

Query: 323 DEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQG 382
           +EQ  I   ++D++ E   LT  D   M    +V++E+LR+AS++ +  R  ++D + +G
Sbjct: 315 EEQMAI---RKDKE-EGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEG 374

Query: 383 YKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAK 439
           Y + +GW V    R++H    I+ +P  F+PSRF+   KP +F+ FG G   C G  LAK
Sbjct: 375 YLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAK 432

[Close]

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.3e-52
Identity = 141/455 (30.99%), Postives = 222/455 (48.79%), Query Frame = 0


Query: 1   MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF 60
           M FS  F    AAA        L + L   ++  RR SS   P PPG+ G P +GET Q 
Sbjct: 1   MDFSGLFLTLSAAA--------LFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQL 60

Query: 61  MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR 120
                 S     F   +  RYG  FKT + G   V +SS E+AK +L      +   +  
Sbjct: 61  Y-----SQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPA 120

Query: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE------ 180
           S   ++G +++       H  LR  ++  F    + + V   +    E+L  W+      
Sbjct: 121 SKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNT 180

Query: 181 -------TMCKMLMSIQNDDELELLQ--KEVAHVCE-AMLAFPWRFPGTRFHNGLKARRR 240
                  T    L+SI   DE+   +  K   ++ E    + P   PGT FH  +KAR+ 
Sbjct: 181 YQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKE 240

Query: 241 IIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTAS 300
           + +IL  ++ +RR++ + H D L   +     + +  L D +I DNI+ ++ A +DTTAS
Sbjct: 241 LAQILANILSKRRQNPSSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTAS 300

Query: 301 AITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLR 360
            +TW++K+L +NP VL+ + +EQ  I    R   +E   LT ED   M    +V++E+LR
Sbjct: 301 VLTWILKYLADNPTVLEAVTEEQMAI----RKDKKEGESLTWEDTKKMPLTYRVIQETLR 360

Query: 361 LASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKP 420
            A+++ +  R  ++D + +GY + +GW V    R++H +  I+  P  F+PSRF+   KP
Sbjct: 361 AATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKP 420

Query: 421 YSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
            +F+ FG G   C G  LAK  + V +H L T YR
Sbjct: 421 NTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYR 433

[Close]

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot
Match: K4CI52 (Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.4e-51
Identity = 142/453 (31.35%), Postives = 226/453 (49.89%), Query Frame = 0


Query: 5   LCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAV 64
           LC + F +  L      +LI  +F  ++ A    S   P PPG+ GLP IGET Q     
Sbjct: 7   LCLFTFISLTL------LLIHSIFKFLAFA----SKKLPLPPGTLGLPYIGETFQLY--- 66

Query: 65  NSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAE 124
             S     F   +  +YG  FKT I G   V +SS E+AKQ+L      +   +  S   
Sbjct: 67  --SQNPNVFFASKVKKYGSIFKTYILGCPCVMISSPEAAKQVLVTKANLFKPTFPASKER 126

Query: 125 LVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE---------- 184
           ++G +++       H  LR  ++  F    + + +   +   + +L  ++          
Sbjct: 127 MLGKQAIFFHQGDYHAKLRKLVLQAFKPDSIRNIIPDIESIAITSLESFQGRLINTYQEM 186

Query: 185 ---TMCKMLMSIQNDDELELLQKEVAHVCEAML-----AFPWRFPGTRFHNGLKARRRII 244
              T    L+SI   D  E L +E    C  +L     + P   PGT F+  +KAR+ + 
Sbjct: 187 KTYTFNVALISIFGKD--EFLYREELKKCYYILEKGYNSMPINLPGTLFNKAMKARKELA 246

Query: 245 KILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAI 304
           KI+ K+I  RR  +  H D L   +   GD  +  L D +I DN++ ++ A +DTTAS +
Sbjct: 247 KIVAKIISTRREMKIDHGDLLGSFM---GD--KEGLTDEQIADNVIGVIFAARDTTASVL 306

Query: 305 TWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLA 364
           TW++K+L ENP VLQ + +EQ  I+ K ++ + E   L  +D   M    +V++E+LR+A
Sbjct: 307 TWILKYLGENPSVLQAVTEEQENIMRK-KEVNGEEKVLNWQDTRQMPMTTRVIQETLRVA 366

Query: 365 SVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYS 424
           S++ +  R  ++D + +GY + +GW V    R++H  P  +  P  F+PSRF+   KP +
Sbjct: 367 SILSFTFREAVEDVEFEGYLIPKGWKVLPLFRNIHHSPDNFPEPEKFDPSRFEVSPKPNT 426

Query: 425 FLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
           F+ FG G   C G +LAK  +L+ +H L T YR
Sbjct: 427 FMPFGNGVHSCPGNDLAKLEILILVHHLTTKYR 436

[Close]

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.1e-51
Identity = 132/413 (31.96%), Postives = 205/413 (49.64%), Query Frame = 0


Query: 45  PPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAK 104
           PPGS GLP IGET++      +S     F   R  +YG  FKT I G   V +SS E+A+
Sbjct: 50  PPGSMGLPYIGETLRLYTENPNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAAR 109

Query: 105 QILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQ 164
            +L      +   Y  S   ++G  +L       H  L+  + + F  S L   V+  + 
Sbjct: 110 MVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIEL 169

Query: 165 QIVEALRGWETMCKM--------------LMSIQNDDE----LELLQKEVAHVCEAMLAF 224
            +++ L  W +   +              +MS   D E    +++++     +     + 
Sbjct: 170 LVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSM 229

Query: 225 PWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVE 284
           P   PGT FH  +KAR  + + L K+I E+RR     +  L  +L    D  R  L D +
Sbjct: 230 PLDLPGTLFHKSMKARIELSEELRKVI-EKRRENGREEGGLLGVLLGAKDQKRNGLSDSQ 289

Query: 285 IGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTL 344
           I DNI+ ++ A  DTTAS +TW++K+L ++P++LQ +  EQF I  K +  +R    ++ 
Sbjct: 290 IADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRR---ISW 349

Query: 345 EDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSI 404
           ED   M    +V++E+LR ASV+ +  R  +QD +  GY + +GW V    R +H     
Sbjct: 350 EDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEF 409

Query: 405 YHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
           +  P  F+PSRF+   KPY+++ FG G   C G  LAK  ML+ LH L TS+R
Sbjct: 410 FPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 453

[Close]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL
Match: A0A6J1GIG3 (abscisic acid 8'-hydroxylase 1 OS=Cucurbita moschata OX=3662 GN=LOC111454446 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.5e-250
Identity = 438/456 (96.05%), Postives = 439/456 (96.27%), Query Frame = 0


Query: 1   MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF 60
           MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF
Sbjct: 1   MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF 60

Query: 61  MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR 120
           MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR
Sbjct: 61  MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR 120

Query: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWET----- 180
           SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE+     
Sbjct: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWESGSTIV 180

Query: 181 ------------MCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR 240
                       MCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR
Sbjct: 181 LLNQALKITCKAMCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR 240

Query: 241 RIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTA 300
           RIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTA
Sbjct: 241 RIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTA 300

Query: 301 SAITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESL 360
           SAITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESL
Sbjct: 301 SAITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESL 360

Query: 361 RLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAK 420
           RLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAK
Sbjct: 361 RLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAK 420

Query: 421 PYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
           PYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR
Sbjct: 421 PYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 456

[Close]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL
Match: A0A6J1KJ46 (abscisic acid 8'-hydroxylase 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496178 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 7.4e-237
Identity = 418/457 (91.47%), Postives = 426/457 (93.22%), Query Frame = 0


Query: 1   MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF 60
           MPFSLCFWCFPAAA GGAALSILIVFLFS+ SS RRPSS S PPPPGSRGLPLIGETIQF
Sbjct: 1   MPFSLCFWCFPAAAFGGAALSILIVFLFSMKSSVRRPSSDSSPPPPGSRGLPLIGETIQF 60

Query: 61  MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR 120
           MAAVNSSNGVYDFVRIRCLRYG CFKTRIFGETHVFVSSTESAKQILND GVNYTKKYIR
Sbjct: 61  MAAVNSSNGVYDFVRIRCLRYGGCFKTRIFGETHVFVSSTESAKQILNDGGVNYTKKYIR 120

Query: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWET----- 180
           SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE+     
Sbjct: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWESGSTIV 180

Query: 181 ------------MCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR 240
                       MCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR
Sbjct: 181 LLNQALKITCKAMCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARR 240

Query: 241 RIIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTA 300
           RIIKILEKMI ER+RSEAWHDDFLQRLL+EEGDD RGAL DVEIGDNILTMLIAGQDTTA
Sbjct: 241 RIIKILEKMIWERQRSEAWHDDFLQRLLSEEGDDGRGALSDVEIGDNILTMLIAGQDTTA 300

Query: 301 SAITWMVKFLDENPDVLQNLKDEQFEIL-NKQRDRDRESCFLTLEDLGNMCYAPKVVKES 360
           SAITWMVKFLDENPDVLQNLKDEQFEIL NK RDRDRE+CFLTLEDLGNMCYAPKVVKES
Sbjct: 301 SAITWMVKFLDENPDVLQNLKDEQFEILMNKHRDRDRENCFLTLEDLGNMCYAPKVVKES 360

Query: 361 LRLASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEA 420
           LRLASVVPWFPRLVLQDSQIQGYKVN+GWNVNIDVRSLHSHPSI+HHP IF+PSRF EEA
Sbjct: 361 LRLASVVPWFPRLVLQDSQIQGYKVNKGWNVNIDVRSLHSHPSIFHHPTIFDPSRFYEEA 420

Query: 421 KPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
           KPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR
Sbjct: 421 KPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 457

[Close]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL
Match: A0A6J1KNK0 (abscisic acid 8'-hydroxylase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496178 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.3e-220
Identity = 389/425 (91.53%), Postives = 396/425 (93.18%), Query Frame = 0


Query: 33  SARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGE 92
           S RRPSS S PPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYG CFKTRIFGE
Sbjct: 18  SVRRPSSDSSPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGGCFKTRIFGE 77

Query: 93  THVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST 152
           THVFVSSTESAKQILND GVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST
Sbjct: 78  THVFVSSTESAKQILNDGGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST 137

Query: 153 SFLASFVTQFDQQIVEALRGWET-----------------MCKMLMSIQNDDELELLQKE 212
           SFLASFVTQFDQQIVEALRGWE+                 MCKMLMSIQNDDELELLQKE
Sbjct: 138 SFLASFVTQFDQQIVEALRGWESGSTIVLLNQALKITCKAMCKMLMSIQNDDELELLQKE 197

Query: 213 VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHDDFLQRLLTEEG 272
           VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMI ER+RSEAWHDDFLQRLL+EEG
Sbjct: 198 VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIWERQRSEAWHDDFLQRLLSEEG 257

Query: 273 DDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEIL-NKQ 332
           DD RGAL DVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEIL NK 
Sbjct: 258 DDGRGALSDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEILMNKH 317

Query: 333 RDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNEGWNVN 392
           RDRDRE+CFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVN+GWNVN
Sbjct: 318 RDRDRENCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNKGWNVN 377

Query: 393 IDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL 440
           IDVRSLHSHPSI+HHP IF+PSRF EEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL
Sbjct: 378 IDVRSLHSHPSIFHHPTIFDPSRFYEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL 437

[Close]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL
Match: A0A6J1KKX6 (abscisic acid 8'-hydroxylase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496178 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.3e-220
Identity = 389/425 (91.53%), Postives = 396/425 (93.18%), Query Frame = 0


Query: 33  SARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGE 92
           S RRPSS S PPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYG CFKTRIFGE
Sbjct: 91  SVRRPSSDSSPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYGGCFKTRIFGE 150

Query: 93  THVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST 152
           THVFVSSTESAKQILND GVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST
Sbjct: 151 THVFVSSTESAKQILNDGGVNYTKKYIRSIAELVGHRSLLCASHLDHKFLRSHLINLFST 210

Query: 153 SFLASFVTQFDQQIVEALRGWET-----------------MCKMLMSIQNDDELELLQKE 212
           SFLASFVTQFDQQIVEALRGWE+                 MCKMLMSIQNDDELELLQKE
Sbjct: 211 SFLASFVTQFDQQIVEALRGWESGSTIVLLNQALKITCKAMCKMLMSIQNDDELELLQKE 270

Query: 213 VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHDDFLQRLLTEEG 272
           VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMI ER+RSEAWHDDFLQRLL+EEG
Sbjct: 271 VAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIWERQRSEAWHDDFLQRLLSEEG 330

Query: 273 DDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEIL-NKQ 332
           DD RGAL DVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEIL NK 
Sbjct: 331 DDGRGALSDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLKDEQFEILMNKH 390

Query: 333 RDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNEGWNVN 392
           RDRDRE+CFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVN+GWNVN
Sbjct: 391 RDRDRENCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQGYKVNKGWNVN 450

Query: 393 IDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL 440
           IDVRSLHSHPSI+HHP IF+PSRF EEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL
Sbjct: 451 IDVRSLHSHPSIFHHPTIFDPSRFYEEAKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRL 510

[Close]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL
Match: A0A6J1CRF4 (cytochrome P450 90A1 OS=Momordica charantia OX=3673 GN=LOC111013901 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.7e-172
Identity = 321/453 (70.86%), Postives = 359/453 (79.25%), Query Frame = 0


Query: 4   SLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAA 63
           +LCF C  AA          ++FL     +     SHS   PPG+ GLPL+GET+QFMAA
Sbjct: 6   ALCFCCLGAA----------LLFLLRSRPNRSTCPSHS---PPGTTGLPLVGETLQFMAA 65

Query: 64  VNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIA 123
           +N++NGVYDFVRIRCLRYGRCFKTRIFG THVFVSSTESAK IL+D G  +TKKYI SIA
Sbjct: 66  INTTNGVYDFVRIRCLRYGRCFKTRIFGGTHVFVSSTESAKLILSDGGGKFTKKYISSIA 125

Query: 124 ELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWET-------- 183
            L+G RSLLCASH  HKFLR+ L NLFS +FLASFV QFDQQI+ ALR WET        
Sbjct: 126 ALLGGRSLLCASHEYHKFLRARLTNLFSATFLASFVPQFDQQILYALRTWETGSTILLLN 185

Query: 184 ---------MCKMLMSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARRRII 243
                    MCKMLMS+++++ELE+LQKEVAHVCEAMLAFPWRFPGTRFHNGLKAR RII
Sbjct: 186 QALKITCKAMCKMLMSLESENELEVLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARERII 245

Query: 244 KILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAI 303
           ++LEK+IRERRRS+A H+DFLQRLL EE  +  G L D EI DNILTMLIAGQDTTASAI
Sbjct: 246 EVLEKVIRERRRSKAQHEDFLQRLLKEE--EGGGTLSDAEIVDNILTMLIAGQDTTASAI 305

Query: 304 TWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLA 363
           TWMVKFLDEN DVLQNL++EQ EIL K+      S  LTLEDLGNM YA K+VKESLRLA
Sbjct: 306 TWMVKFLDENQDVLQNLREEQLEILGKR----ASSSILTLEDLGNMSYAAKIVKESLRLA 365

Query: 364 SVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYS 423
           SVVPWFPRLVLQDS IQGYK+ +GWNVNIDVRSLH+ P +Y+ P  FNP RFDEE K YS
Sbjct: 366 SVVPWFPRLVLQDSHIQGYKIKKGWNVNIDVRSLHTDPCVYNDPAKFNPYRFDEEGKAYS 425

Query: 424 FLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
           FLAFGMGGRQCLGMN+AKAMMLVFLHRLVTSYR
Sbjct: 426 FLAFGMGGRQCLGMNMAKAMMLVFLHRLVTSYR 439

[Close]

BLAST of CmoCh12G004020 vs. TAIR 10
Match: AT1G19630.1 (cytochrome P450, family 722, subfamily A, polypeptide 1 )

HSP 1 Score: 465.3 bits (1196), Expect = 5.4e-131
Identity = 248/443 (55.98%), Postives = 310/443 (69.98%), Query Frame = 0


Query: 18  AALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIR 77
           AA+  L  FL  +M   R+ S+     PPGS G P+IGET+QFM +VNS  G Y+FVR R
Sbjct: 12  AAMLTLGKFL-KIMFQDRKKSTAG--VPPGSDGFPVIGETLQFMLSVNSGKGFYEFVRSR 71

Query: 78  CLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHL 137
            +RYG CF+T +FGETHVF+S+TESA+ +LN+    +TK+YI+SI ELVG RSLLCA   
Sbjct: 72  RIRYGSCFRTSLFGETHVFLSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCAPQH 131

Query: 138 DHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE-----------------TMCKML 197
            HK LRS LINLFS    A  V  FD+ +V+AL GWE                  MCKML
Sbjct: 132 HHKILRSRLINLFSKRSTALMVRHFDELVVDALGGWEHRGTVVLLTDLLQITFKAMCKML 191

Query: 198 MSIQNDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRS- 257
           +S++ ++EL  +Q++V  VCEAMLAFP   P TRFH G+ AR R++++LEK+IRERR   
Sbjct: 192 VSLEKEEELGSMQRDVGFVCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRERRNEI 251

Query: 258 ---EAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDEN 317
                 H+DFLQ+LL  + D  +  L D EI DNILTM+IAGQDTTASA+TWMVK+L EN
Sbjct: 252 NSHNNHHEDFLQQLLAVDNDTPQ--LTDAEIKDNILTMIIAGQDTTASALTWMVKYLGEN 311

Query: 318 PDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLV 377
             VL  L +EQ +I  K  ++     FL LEDL  M YA K+VKESLR+ASVVPWFPRLV
Sbjct: 312 QKVLDILIEEQSQITKKASNKP----FLELEDLSEMPYASKMVKESLRMASVVPWFPRLV 371

Query: 378 LQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQ 437
           LQD +++GYK+ +GWN+NID RS+H  P++Y  P  FNP RF+EEAK  SFLAFGMGGR 
Sbjct: 372 LQDCEMEGYKIKKGWNINIDARSIHLDPTVYSEPHKFNPLRFEEEAKANSFLAFGMGGRT 431

Query: 438 CLGMNLAKAMMLVFLHRLVTSYR 440
           CLG+ LAKAMMLVFLHR +T+YR
Sbjct: 432 CLGLALAKAMMLVFLHRFITTYR 445

[Close]

BLAST of CmoCh12G004020 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-54
Identity = 138/435 (31.72%), Postives = 216/435 (49.66%), Query Frame = 0


Query: 22  ILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRY 81
           IL + L  V+ S ++ +S     PPGS G P +GET+Q       S     F   +  RY
Sbjct: 13  ILCLLLVRVIVSKKKKNSRG-KLPPGSMGWPYLGETLQLY-----SQNPNVFFTSKQKRY 72

Query: 82  GRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKF 141
           G  FKTRI G   V ++S E+A+ +L      +   Y RS  +L+G  +L       H  
Sbjct: 73  GEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSH 132

Query: 142 LRSHLINLFSTSFLASFVTQFDQQIVEALRGWETM-----------------CKMLMSIQ 201
           +R  + + F    +   +   +   + +L+ W  M                    +    
Sbjct: 133 IRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHL 192

Query: 202 NDDELELLQKEVAHVCEAMLAFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHD 261
                E+L+     V +   +FP   PGT +H  L AR+++  I+ ++I ERR   A   
Sbjct: 193 ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQT 252

Query: 262 DFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLK 321
           DFL  LL  + +  R  L   +I DNI+ +L A QDTTAS +TW++K+L ++  +L+ +K
Sbjct: 253 DFLGHLLNFKNEKGR-VLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVK 312

Query: 322 DEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQG 381
            EQ  I     +  RE   LT     NM    KV+ ESLR+AS++ +  R  + D + +G
Sbjct: 313 AEQKAI---YEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKG 372

Query: 382 YKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAK 440
           Y + +GW V    R++H +P  + +P +F+PSRF+   KP +F+ FG G   C G  LAK
Sbjct: 373 YLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAK 432

[Close]

BLAST of CmoCh12G004020 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 209.9 bits (533), Expect = 4.1e-54
Identity = 137/435 (31.49%), Postives = 224/435 (51.49%), Query Frame = 0


Query: 23  LIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYG 82
           L ++    + S RR  S   P PPG+ G P +GET Q       S     F + +  RYG
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRYG 74

Query: 83  RCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFL 142
             FKT + G   V +SS E+AK +L      +   +  S   ++G +++       H  L
Sbjct: 75  SVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKL 134

Query: 143 RSHLINLFSTSFLASFVTQFDQQIVEALRGWE-------------TMCKMLMSIQNDDEL 202
           R  ++  F    + + V   +    ++LR WE             T    L+SI   D  
Sbjct: 135 RKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKD-- 194

Query: 203 ELLQKEVAHVCEAML-----AFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHD 262
           E+L +E    C  +L     + P   PGT FH  +KAR+ + +IL +++ ERR++ + H+
Sbjct: 195 EVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHN 254

Query: 263 DFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLK 322
           D L   + ++ +     L D +I DNI+ ++ A +DTTAS ++W++K+L ENP+VL+ + 
Sbjct: 255 DLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVT 314

Query: 323 DEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQG 382
           +EQ  I   ++D++ E   LT  D   M    +V++E+LR+AS++ +  R  ++D + +G
Sbjct: 315 EEQMAI---RKDKE-EGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEG 374

Query: 383 YKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAK 440
           Y + +GW V    R++H    I+ +P  F+PSRF+   KP +F+ FG G   C G  LAK
Sbjct: 375 YLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAK 433

[Close]

BLAST of CmoCh12G004020 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 208.0 bits (528), Expect = 1.6e-53
Identity = 136/434 (31.34%), Postives = 223/434 (51.38%), Query Frame = 0


Query: 23  LIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQFMAAVNSSNGVYDFVRIRCLRYG 82
           L ++    + S RR  S   P PPG+ G P +GET Q       S     F + +  RYG
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRYG 74

Query: 83  RCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIRSIAELVGHRSLLCASHLDHKFL 142
             FKT + G   V +SS E+AK +L      +   +  S   ++G +++       H  L
Sbjct: 75  SVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKL 134

Query: 143 RSHLINLFSTSFLASFVTQFDQQIVEALRGWE-------------TMCKMLMSIQNDDEL 202
           R  ++  F    + + V   +    ++LR WE             T    L+SI   D  
Sbjct: 135 RKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKD-- 194

Query: 203 ELLQKEVAHVCEAML-----AFPWRFPGTRFHNGLKARRRIIKILEKMIRERRRSEAWHD 262
           E+L +E    C  +L     + P   PGT FH  +KAR+ + +IL +++ ERR++ + H+
Sbjct: 195 EVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHN 254

Query: 263 DFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTASAITWMVKFLDENPDVLQNLK 322
           D L   + ++ +     L D +I DNI+ ++ A +DTTAS ++W++K+L ENP+VL+ + 
Sbjct: 255 DLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVT 314

Query: 323 DEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLRLASVVPWFPRLVLQDSQIQG 382
           +EQ  I   ++D++ E   LT  D   M    +V++E+LR+AS++ +  R  ++D + +G
Sbjct: 315 EEQMAI---RKDKE-EGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEG 374

Query: 383 YKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMGGRQCLGMNLAK 439
           Y + +GW V    R++H    I+ +P  F+PSRF+   KP +F+ FG G   C G  LAK
Sbjct: 375 YLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAK 432

[Close]

BLAST of CmoCh12G004020 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 206.1 bits (523), Expect = 5.9e-53
Identity = 141/455 (30.99%), Postives = 222/455 (48.79%), Query Frame = 0


Query: 1   MPFSLCFWCFPAAALGGAALSILIVFLFSVMSSARRPSSHSPPPPPGSRGLPLIGETIQF 60
           M FS  F    AAA        L + L   ++  RR SS   P PPG+ G P +GET Q 
Sbjct: 1   MDFSGLFLTLSAAA--------LFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQL 60

Query: 61  MAAVNSSNGVYDFVRIRCLRYGRCFKTRIFGETHVFVSSTESAKQILNDSGVNYTKKYIR 120
                 S     F   +  RYG  FKT + G   V +SS E+AK +L      +   +  
Sbjct: 61  Y-----SQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPA 120

Query: 121 SIAELVGHRSLLCASHLDHKFLRSHLINLFSTSFLASFVTQFDQQIVEALRGWE------ 180
           S   ++G +++       H  LR  ++  F    + + V   +    E+L  W+      
Sbjct: 121 SKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNT 180

Query: 181 -------TMCKMLMSIQNDDELELLQ--KEVAHVCE-AMLAFPWRFPGTRFHNGLKARRR 240
                  T    L+SI   DE+   +  K   ++ E    + P   PGT FH  +KAR+ 
Sbjct: 181 YQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKE 240

Query: 241 IIKILEKMIRERRRSEAWHDDFLQRLLTEEGDDSRGALLDVEIGDNILTMLIAGQDTTAS 300
           + +IL  ++ +RR++ + H D L   +     + +  L D +I DNI+ ++ A +DTTAS
Sbjct: 241 LAQILANILSKRRQNPSSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTAS 300

Query: 301 AITWMVKFLDENPDVLQNLKDEQFEILNKQRDRDRESCFLTLEDLGNMCYAPKVVKESLR 360
            +TW++K+L +NP VL+ + +EQ  I    R   +E   LT ED   M    +V++E+LR
Sbjct: 301 VLTWILKYLADNPTVLEAVTEEQMAI----RKDKKEGESLTWEDTKKMPLTYRVIQETLR 360

Query: 361 LASVVPWFPRLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKP 420
            A+++ +  R  ++D + +GY + +GW V    R++H +  I+  P  F+PSRF+   KP
Sbjct: 361 AATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKP 420

Query: 421 YSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYR 440
            +F+ FG G   C G  LAK  + V +H L T YR
Sbjct: 421 NTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYR 433

The following BLAST results are available for this feature:

BLAST of CmoCh12G004020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJK2	3.4e-53	31.72	Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ...	[more]
Q949P1	2.2e-52	31.34	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ...	[more]
Q9FH76	8.3e-52	30.99	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ...	[more]
K4CI52	2.4e-51	31.35	Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
O81077	4.1e-51	31.96	Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ...	[more]

BLAST of CmoCh12G004020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GIG3	1.5e-250	96.05	abscisic acid 8'-hydroxylase 1 OS=Cucurbita moschata OX=3662 GN=LOC111454446 PE=...	[more]
A0A6J1KJ46	7.4e-237	91.47	abscisic acid 8'-hydroxylase 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1KNK0	6.3e-220	91.53	abscisic acid 8'-hydroxylase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1KKX6	6.3e-220	91.53	abscisic acid 8'-hydroxylase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1CRF4	3.7e-172	70.86	cytochrome P450 90A1 OS=Momordica charantia OX=3673 GN=LOC111013901 PE=3 SV=1	[more]

BLAST of CmoCh12G004020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19630.1	5.4e-131	55.98	cytochrome P450, family 722, subfamily A, polypeptide 1	[more]
AT3G19270.1	2.4e-54	31.72	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT4G19230.2	4.1e-54	31.49	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.1	1.6e-53	31.34	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.1	5.9e-53	30.99	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 417..428 score: 36.7 coord: 277..294 score: 34.76 coord: 337..348 score: 38.43 coord: 408..417 score: 51.83
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 197..438 e-value: 5.9E-55 score: 186.8
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 417..439 score: 26.88 coord: 376..400 score: 26.63 coord: 407..417 score: 49.77 coord: 336..354 score: 27.75 coord: 266..283 score: 26.63 coord: 286..312 score: 20.16
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 34..439 e-value: 2.6E-97 score: 328.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..438
None	No IPR available	PANTHER	PTHR24286:SF189	CYTOCHROME P450, FAMILY 722, SUBFAMILY A, POLYPEPTIDE 1	coord: 22..438
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 22..438
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 410..419

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G004020.1	CmoCh12G004020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen