CmoCh12G003620 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh12G003620 vs. ExPASy Swiss-Prot
Match: Q54UB1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyostelium discoideum OX=44689 GN=trmt6 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.1e-54
Identity = 144/432 (33.33%), Postives = 233/432 (53.94%), Query Frame = 0

Query: 19  EGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRPFGSLFQIESGDNGQHLAC 78
           EG  V+LDIN+G++    ++   S +KIG K   +  +IG  + S FQ+ +  N   L  
Sbjct: 37  EGDHVILDINNGEKFSVIKVKLGSKVKIGKKQILINSIIGESYYSSFQVSNEKN--TLER 96

Query: 79  VTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDIDAMRSQGATGDEIVEALI 138
           +T  ++E  + +     +     DNR L  NN AQ L+ EDI+ M+ +G   + I++ ++
Sbjct: 97  IT--QKEIDDRLNNLVELNQNDADNRNLDQNNTAQKLTQEDINEMKQKGTDSNTIIKTIV 156

Query: 139 ANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFLRVDALS 198
            NS +F+ KT FSQ KY  KK KKY+  V + +P  +S+ EAY+KK   +I  LR D+  
Sbjct: 157 ENSESFKTKTSFSQIKYLKKKIKKYSTIVKIIKPTLKSLTEAYYKKDSRKICGLRFDSFG 216

Query: 199 LLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVRMFNFDD 258
            LL+  N+ +NS VLV +   GL+TG++AER+ G+G++ + Y+G  P+ + IV  F F+ 
Sbjct: 217 QLLTLGNIRANSQVLVVETCMGLVTGSIAERMNGQGTILSAYIGKGPS-LSIVNNFGFNT 276

Query: 259 EICARIMRSPLTDLTS------------SQVSNGEHEVTIKAQDEASEPVNMDEGPLPST 318
           ++   I    L ++TS               S+G ++  +K +++  E    DE      
Sbjct: 277 DVLNTIYPFNL-NITSVLNKGEDISKLPPTASSGIYDKQVKEKEKEKE---KDEN--VKD 336

Query: 319 DQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAWGLVKDL 378
           ++ S   +++I++         +  D +   +VK  K+ G  SL+I    S    ++   
Sbjct: 337 EKESGEEAKTIIN---------QRNDTSNENIVKLLKDGGVWSLVIVTKYSPL-NILLSC 396

Query: 379 LPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMN 438
            P + +S  F IY Q+ QPL      L   +MA++ QISE W+RE+QVLP RTHP M M+
Sbjct: 397 WPYLNSSGSFVIYSQFPQPLMEVHQFLHKNQMAVNQQISEIWMREHQVLPKRTHPMMGMD 447

BLAST of CmoCh12G003620 vs. ExPASy Swiss-Prot
Match: Q8CE96 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musculus OX=10090 GN=Trmt6 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.7e-48
Identity = 139/454 (30.62%), Postives = 213/454 (46.92%), Query Frame = 0

Query: 27  INDGDRLVFARLSPASTLKIGNKS--------YSLKPLIGRPFGSLFQIESGDNGQHLAC 86
           I+DGD +V  R      +++  +         + L   IG  +GS F + SG + Q    
Sbjct: 21  IHDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNAIGHSYGSAFDVSSGGSLQ---- 80

Query: 87  VTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDIDAMRSQGATGDEIVEALI 146
           +    EE   E KE         DNR +VD+ K+Q L+ +DI A++ +G  G+EIV+ LI
Sbjct: 81  LRKKLEEPASETKEAGT------DNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLI 140

Query: 147 ANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFLRVDALS 206
            NS TF  KT F+Q+KY  KK+KKY   V + +P TR +   Y+ + P +I  +R D L+
Sbjct: 141 ENSTTFRDKTEFAQDKYIKKKKKKYEAIVTILKPSTRILSIMYYAREPGKINHMRYDTLA 200

Query: 207 LLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVRMFNFDD 266
            +L+  N+ + + ++V +   GL+ GA+ ER+GG GS+   Y G  P        F F  
Sbjct: 201 QMLTLGNIRAGNKMIVMETCSGLVLGAMMERMGGFGSIIQLYPGDGP-VRAATACFGFPK 260

Query: 267 EICARIMRSPLTDLTSSQVSNGEHEV-TIKAQDEASEPVNMDEGPLPSTDQSSDLNSESI 326
              + +   PL  + S  + NG      + ++ + S PV    G L   + +   + ++I
Sbjct: 261 SFLSGLYEFPLNKVNS--LLNGTFSAEMLSSEPKDSTPVEESNGELEEKEIAEQADEDNI 320

Query: 327 VSPLTKVLKCAKAGDKAPPEVVKSWKEN-------------------------------- 386
           V           +G++ P E+V    EN                                
Sbjct: 321 VDAAEN-----NSGEQRPMEIVPGDPENKEPKEKRSKRDYIQEKQRRQEEQRKRHLEAAA 380

Query: 387 ----GFTSLIIAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAIS 436
                    +I A +     L+  LL  VA S PF +Y QY +PL  C  KL+     I+
Sbjct: 381 LLGERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVIN 440

BLAST of CmoCh12G003620 vs. ExPASy Swiss-Prot
Match: Q2T9V5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taurus OX=9913 GN=TRMT6 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.3e-47
Identity = 138/449 (30.73%), Postives = 215/449 (47.88%), Query Frame = 0

Query: 27  INDGDRLVFARLSPASTLKIGNKS--------YSLKPLIGRPFGSLFQIESGDNGQHLAC 86
           I DGD +V  R      +++  +         + L  +IG  +G+ F++ +G + Q    
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNIIGHSYGTTFEVTNGGSLQ---- 79

Query: 87  VTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDIDAMRSQGATGDEIVEALI 146
               +EE T E KE         DNR ++D+ K+Q L+ +DI A++ +G  G+EIV+ LI
Sbjct: 80  PKKKKEEPTSETKEAGT------DNRNIIDDGKSQKLTQDDIKALKDKGIKGEEIVQQLI 139

Query: 147 ANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFLRVDALS 206
            NS TF  KT F+Q+KY  KK+KKY   + + +P TR +   Y+ + P +I  +R D L+
Sbjct: 140 ENSTTFRDKTEFAQDKYIKKKKKKYEAMITVVKPSTRILSVMYYAREPGKINHMRYDTLA 199

Query: 207 LLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVRMFNFDD 266
            +L+  N+ + + ++V +   GL+ GA+ ER+GG GS+   Y G  P        F F  
Sbjct: 200 QMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATACFGFPK 259

Query: 267 EICARIMRSPLT--------------------DLTSSQVSNG----------EHEVTIKA 326
              + +   PL                     D+ S + SNG          E+E +I  
Sbjct: 260 SFLSGLYEFPLNKVDSLLNGTFSAEMLSSEPKDIASVEESNGTLEEKQTSEQENEDSIAE 319

Query: 327 QDEASEPVNMDEGPLPS--TDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENG 386
             E++ P   +   + S   D      S S    + +  +  +   K   E      E  
Sbjct: 320 APESNHPEEQERMEIVSQDPDYKEPKESGSKKDYIQEKQRRQEEQKKRHLEAAALLSERN 379

Query: 387 FTSLIIAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISE 436
              LI+A+ +     L+  LL  VA S PF +Y QY +PL  C  KL+     I+L++SE
Sbjct: 380 ADGLIVAS-RFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSE 439

BLAST of CmoCh12G003620 vs. ExPASy Swiss-Prot
Match: Q9UJA5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sapiens OX=9606 GN=TRMT6 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.1e-46
Identity = 138/449 (30.73%), Postives = 212/449 (47.22%), Query Frame = 0

Query: 27  INDGDRLVFARLSPASTLKIGNKS--------YSLKPLIGRPFGSLFQIESGDNGQHLAC 86
           I DGD +V  R      +++  +         + L  +IG  +G+ F++ SG + Q    
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQ---- 79

Query: 87  VTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDIDAMRSQGATGDEIVEALI 146
               REE T E KE         DNR +VD+ K+Q L+ +DI A++ +G  G+EIV+ LI
Sbjct: 80  PKKKREEPTAETKEAGT------DNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLI 139

Query: 147 ANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFLRVDALS 206
            NS TF  KT F+Q+KY  KK+KKY   + + +P TR +   Y+ + P +I  +R D L+
Sbjct: 140 ENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLA 199

Query: 207 LLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVRMFNFDD 266
            +L+  N+ + + ++V +   GL+ GA+ ER+GG GS+   Y G  P        F F  
Sbjct: 200 QMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATACFGFPK 259

Query: 267 EICARIMRSPLTDLTS------------------------------SQVSNGEHEVTIKA 326
              + +   PL  + S                               Q S  E+E ++  
Sbjct: 260 SFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAE 319

Query: 327 QDEASEPVNMDEGPLPSTDQSSDLNSE--SIVSPLTKVLKCAKAGDKAPPEVVKSWKENG 386
             E++ P + +     S D       E  S    + +  +  +   K   E      E  
Sbjct: 320 APESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERN 379

Query: 387 FTSLIIAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISE 436
              LI+A+ +     L+  LL  VA S PF +Y QY +PL  C  KL+     I+L++SE
Sbjct: 380 ADGLIVAS-RFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSE 439

BLAST of CmoCh12G003620 vs. ExPASy Swiss-Prot
Match: Q6BKK7 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=TRM6 PE=3 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 5.0e-36
Identity = 112/414 (27.05%), Postives = 205/414 (49.52%), Query Frame = 0

Query: 38  LSPASTLKIGN-KSYSLKPLIGRPFGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVV 97
           L P   + +G   ++++  ++G PFG  F+I      + +  +T   +E + + + E++ 
Sbjct: 50  LKPGGIISLGKFGTFAVDGILGYPFGQTFEILEELKVKPIKSMTMQADETSVDNENEELT 109

Query: 98  KDEL-------RDNRALVDN--NKAQSLSSEDIDAMRSQGAT---GDEIVEALIANSATF 157
           KD+L        DN   + N  +K Q L+SED+D ++  GAT   G  I+E +IA    F
Sbjct: 110 KDDLTKMLSNSSDNNQNIINIGSKIQKLTSEDVDKLKESGATSDIGQRIIEQMIAGHEGF 169

Query: 158 EKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFLRVDALSLLLSFA 217
           +KKT+FSQ+KY  +KQ+K+  R  +    +  + + Y +K   R+  +  + L LLL++A
Sbjct: 170 DKKTLFSQQKYLKRKQQKFLRRFTVEYLGSSQLLQYYIEKDTQRVLDMSEETLGLLLNYA 229

Query: 218 NVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVRMFNFDDEICARI 277
           NV      L+ D  GG+I  A+ ER+ G+G++ + +   +PN + + R  ++ +E+  R+
Sbjct: 230 NVRPGGKYLLIDETGGIILYAMMERMNGQGTIVSAHDNEHPNHIAL-RYSDYPEEMQNRM 289

Query: 278 MRSPLTDLTSSQVSNGEHEVTIKAQDEASEPVNMDEGPLPSTDQSSDLNSESIVSPLTKV 337
           ++S +  L   +  N + E     ++E  E  +         +Q +  N  S++      
Sbjct: 290 VKS-INWLQFLEPENEKVEFETATEEEIEEMKHAKRAQYYRREQRAK-NINSVI------ 349

Query: 338 LKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQ 397
                       ++V     +GF S+      +    L+ +++P +  S P  IY Q+ +
Sbjct: 350 ------------DMVMEGNFDGFISVSTLNMPT----LLPEIIPTIGGSRPVVIYSQFKE 409

Query: 398 PLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAFGGYILSGTKI 439
            L    H +  +K  ++  I E  +R +Q +P R HP M M  FGGYIL GT++
Sbjct: 410 ALLETQHHMSTDKRVLAPSIMETRVRPHQTIPGRMHPVMCMRGFGGYILWGTRV 438

BLAST of CmoCh12G003620 vs. ExPASy TrEMBL
Match: A0A6J1GI99 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbita moschata OX=3662 GN=LOC111454455 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 9.2e-251
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60
           MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP
Sbjct: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60

Query: 61  FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI 120
           FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI
Sbjct: 61  FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI 120

Query: 121 DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA 180
           DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA
Sbjct: 121 DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA 180

Query: 181 YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTY 240
           YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTY
Sbjct: 181 YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTY 240

Query: 241 LGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVSNGEHEVTIKAQDEASEPVNMDE 300
           LGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVSNGEHEVTIKAQDEASEPVNMDE
Sbjct: 241 LGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVSNGEHEVTIKAQDEASEPVNMDE 300

Query: 301 GPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW 360
           GPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW
Sbjct: 301 GPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW 360

Query: 361 GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH 420
           GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH
Sbjct: 361 GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH 420

Query: 421 PFMQMNAFGGYILSGTKICDVAHQQAI 448
           PFMQMNAFGGYILSGTKICDVAHQQAI
Sbjct: 421 PFMQMNAFGGYILSGTKICDVAHQQAI 447

BLAST of CmoCh12G003620 vs. ExPASy TrEMBL
Match: A0A6J1KL25 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbita maxima OX=3661 GN=LOC111496211 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.4e-245
Identity = 438/444 (98.65%), Postives = 438/444 (98.65%), Query Frame = 0

Query: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60
           MSSSNL SESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP
Sbjct: 1   MSSSNLPSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60

Query: 61  FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI 120
           FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI
Sbjct: 61  FGSLFQIESGDNGQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDI 120

Query: 121 DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA 180
           DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA
Sbjct: 121 DAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA 180

Query: 181 YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTY 240
           YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCN Y
Sbjct: 181 YFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNAY 240

Query: 241 LGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVSNGEHEVTIKAQDEASEPVNMDE 300
           LGSNPNPMEIVRMFNFDDEICARIMRSPLTDL SSQVSNGEHEV IKAQDEASEPVNMDE
Sbjct: 241 LGSNPNPMEIVRMFNFDDEICARIMRSPLTDLASSQVSNGEHEVNIKAQDEASEPVNMDE 300

Query: 301 GPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW 360
           GP PSTDQSS LNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW
Sbjct: 301 GPPPSTDQSSGLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAW 360

Query: 361 GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH 420
           GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH
Sbjct: 361 GLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH 420

Query: 421 PFMQMNAFGGYILSGTKICDVAHQ 445
           PFMQMNAFGGYILSGTKICDVAHQ
Sbjct: 421 PFMQMNAFGGYILSGTKICDVAHQ 444

BLAST of CmoCh12G003620 vs. ExPASy TrEMBL
Match: A0A0A0LRN2 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis sativus OX=3659 GN=Csa_2G367740 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.6e-216
Identity = 390/450 (86.67%), Postives = 415/450 (92.22%), Query Frame = 0

Query: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60
           MS + LQSES RNPR+ WEGCSVLLD+NDGDRLVFARLSPASTLKIGNKS+SLKPLIG P
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIESGDNGQHLACVTPSREE------ETEEMKEEQVVKDELRDNRALVDNNKAQS 120
           FGSLFQIE+G NG  LA V PSREE      ETEEMKEEQV KDELRDNRA+VDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKG 240
           RSICEAYFKK+PARIGFLR+DALSLLLSF NVT+NSDVLV DMVGGL+TGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVS----NGEHEVTIKAQD 300
           SVCNTY+G+ PNPM+IVR+FNFDDEIC+RIMRS L DLTSSQ+     NGE+E +I  Q+
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 EASEPVNMDEGPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSL 360
           +AS+PV+MDE PLPSTDQSSDLNSESIVSPLTKV KCAKAGDKAPPEV+  WKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSDAW LVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICD 441
           EYQVLPSRTHPFMQMNAFGGYILSGTKICD
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICD 450

BLAST of CmoCh12G003620 vs. ExPASy TrEMBL
Match: A0A6J1CS60 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Momordica charantia OX=3673 GN=LOC111014079 PE=3 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.6e-215
Identity = 392/448 (87.50%), Postives = 412/448 (91.96%), Query Frame = 0

Query: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60
           MSS NLQSE  RNPRV WEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIG P
Sbjct: 1   MSSDNLQSELHRNPRVTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGCP 60

Query: 61  FGSLFQIESGDNGQHLACVTPSREE-----ETEEMKEEQVVKDELRDNRALVDNNKAQSL 120
           FGSLFQIE+   G HL+ V PSREE     ETE+MKEEQV KDELRDNRA+VDNNKAQSL
Sbjct: 61  FGSLFQIENVAGGPHLSRVMPSREEGINCQETEKMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRL+KQKKYAPRVL+RRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLVRRPFTR 180

Query: 181 SICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGS 240
           SICEAYFKK+PARIGFLRVDALSLLLSFANVTSNSDVLV DMVGGL+TGAVAERLGGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTSNSDVLVLDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQV----SNGEHEVTIKAQDE 300
           VCNTYLG+NPN M+IVR+FNFDDEICARIMRSPL DL SSQV    SNGEHE +I A+++
Sbjct: 241 VCNTYLGNNPNHMDIVRIFNFDDEICARIMRSPLIDLASSQVDSSKSNGEHEASINAEEQ 300

Query: 301 ASEPVNMDEGPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLI 360
           ASEPV+MDE  +PSTDQ S+LNSESIVSPLTK  KCAKAGDKAP EVV SWKENGFTSLI
Sbjct: 301 ASEPVSMDESAVPSTDQPSELNSESIVSPLTKAFKCAKAGDKAPSEVVNSWKENGFTSLI 360

Query: 361 IAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQ DAW LVKDLLPLV+NSAPFAIYHQYLQPLATCMHKLQLE+MAISLQISEPWLRE
Sbjct: 361 IAAPQLDAWSLVKDLLPLVSNSAPFAIYHQYLQPLATCMHKLQLERMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 440
           YQVLPSRTHPFMQMNAFGGYILSGTKIC
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 448

BLAST of CmoCh12G003620 vs. ExPASy TrEMBL
Match: A0A1S3BB68 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis melo OX=3656 GN=LOC103488037 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.2e-212
Identity = 385/448 (85.94%), Postives = 415/448 (92.63%), Query Frame = 0

Query: 2   SSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRPF 61
           S++ LQS+S RNPR+ WEGCSVLLD+NDGDRLVFARLSPASTLKIGNKS+SLKPLIG PF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIESGDNGQHLACVTPSREE-----ETEEMKEEQVVKDELRDNRALVDNNKAQSLS 121
           GSLFQIE+G+NG  LA V PSREE     ETEEMKEEQV KDELRDNRALVDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSV 241
           ICEAYFKK+PARIGFLR+DALSLLLSF NVT+NSDVLV DMVGGL+TGAVAERLGGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVS----NGEHEVTIKAQDEA 301
           CNTY+G+ PNPM+IVR+FNFDDEIC+RIMRS L DLTSSQ+     NGE++V+    ++A
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVS----EQA 302

Query: 302 SEPVNMDEGPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLII 361
           S+PV+MDE PLPSTDQSSDLNSESIVSPLTKV KCAKAGDKAPPEV+  WKENGFTSLII
Sbjct: 303 SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII 362

Query: 362 AAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREY 421
           AAPQSDAW LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREY
Sbjct: 363 AAPQSDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREY 422

Query: 422 QVLPSRTHPFMQMNAFGGYILSGTKICD 441
           QVLPSRTHPFMQMNAFGGYILSGTKICD
Sbjct: 423 QVLPSRTHPFMQMNAFGGYILSGTKICD 446

BLAST of CmoCh12G003620 vs. TAIR 10
Match: AT2G45730.1 (eukaryotic initiation factor 3 gamma subunit family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 5.3e-150
Identity = 271/430 (63.02%), Postives = 338/430 (78.60%), Query Frame = 0

Query: 13  NPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRPFGSLFQIESGDN 72
           NPR A EGCSVLLDINDGDRLVFARLS  + LKIGNK+YSLKPLIG PFGSLFQ+E+G++
Sbjct: 20  NPRFACEGCSVLLDINDGDRLVFARLSSGAILKIGNKNYSLKPLIGAPFGSLFQVETGED 79

Query: 73  GQHLACVTPSREEETEEMKEEQVVKDELRDNRALVDNNKAQSLSSEDIDAMRSQGATGDE 132
           G  L+ + P ++E          V D+ RDNR +VDNN+AQ+L+ E+I+AMR +GA GDE
Sbjct: 80  GSFLSRILPIKQESNSNN-----VMDDSRDNREIVDNNEAQNLTGEEIEAMRREGAKGDE 139

Query: 133 IVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKFPARIGFL 192
           I+EALIANS TF+KK   SQEKY+LKKQKKYAP++LLRRPF RSICEAYFKK+PARIGFL
Sbjct: 140 IIEALIANSKTFDKKFQLSQEKYKLKKQKKYAPKLLLRRPFARSICEAYFKKYPARIGFL 199

Query: 193 RVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGSVCNTYLGSNPNPMEIVR 252
           RVDALSLLL+ ANVT+ SDVLV DMVGGL+TGAVAERLGG G VCNTY GS+P P+E+VR
Sbjct: 200 RVDALSLLLTMANVTAYSDVLVVDMVGGLVTGAVAERLGGTGYVCNTYKGSSPYPVEMVR 259

Query: 253 MFNFDDEICARIMRSPLTDLTSSQVS----NGEHEVTIKAQDEASEPVNMDEGPLPSTDQ 312
           MFNF D++  RI++S + +L+S++ +    N + E   KA++  +E    D   +     
Sbjct: 260 MFNFSDKVLKRIVQSSINELSSAKTASTEGNNQQEGVCKAENNINETATSDV-MVEEISV 319

Query: 313 SSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLIIAAPQSDAWGLVKDLLP 372
           +++     IV P +K++K AKAG KA  E ++ W+ENGF+SLI+AA   D W + KD+LP
Sbjct: 320 TTEAGVVDIVVPESKIIKAAKAGAKASKETIEMWQENGFSSLIMAAQDQDPWIVAKDVLP 379

Query: 373 LVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAF 432
           L++ SAPFAIYHQYLQPLATCMHKLQ  KMAI+LQI+EPWLREYQVLPSR+HP MQM++F
Sbjct: 380 LLSYSAPFAIYHQYLQPLATCMHKLQQGKMAINLQITEPWLREYQVLPSRSHPHMQMSSF 439

Query: 433 GGYILSGTKI 439
           GGY+LSG +I
Sbjct: 440 GGYVLSGIRI 443