CmoCh12G003390 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G003390
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPseudouridine synthase
LocationCmo_Chr12: 2112041 .. 2117544 (-)
RNA-Seq ExpressionCmoCh12G003390
SyntenyCmoCh12G003390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGGCGAAAACCCAAGAACCAAATATCGAACTCGGGAGAATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGTAACGACGATTTTAGCTTGTACTCCCGAAGAACAACTGCATTTGACTCTTCGATTGTCTCTCTGTTTTTCATTTGGCTTCTTTCAACCATATATTTTATGCTCGAGCAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTAATGATTCAAGAGACGTTATGCCTCAAGTTCGATATTGTATGCTTAGTGGCAAGCTTGTGTCTGAAATTTGTAAAAGATTGTGATAGCGGTTTGATTTCTCCTTGATTCAGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACACGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTACTGTCCCTCCTTCAGCTTCCTTGCAGCTGCTTAACTCAAGATCAAAGCAAAACAATTCAAGTTTTGTGTTTCAAGAATTTGCAGCCCAATAGCTCCCCTTTTTTGTCCCCTAACTATATCTAACTCCTACGTTTGTTGCATTTGAATATCAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTTCGTCAATTATTATCATATGAAATGCAATTATAAATCTACTGGTTCTTGAAATGCTGTCTTCCCCATGTTACTACCCTCATTTCGTATTGGAGGTATTTTTTTATAACTGTGTACAGACAGGGTCTTTTTTGTATTCATTTCATCAGATTAATGAAATGGGTTCTGTTTCCTGTAAAAAAATCATCTACATATAAGGTCAGAACTAAAAGGTAATGACAATATACTTGTCATCATCATAAATTTGGATAACTAATATTTAGAAAGAAAACTCCACATTTCCGACAGACTGCTCAAACTCTTGGCTGAACTGGTTGTTTATATGAAGAGGTTAAGGTGGTGCTTCTAGAGACATTGTCATGTTGGTGGCTTAAAGGTAAAGCTAGGACTATTTGAAAGAATGCAACTTGAGCCATCCTTTGAAGTATATAAATAGAAAGGAATAACATAATTTTCAAAGAGAGTAACAAAGCAGTAAGCTTTGGAGTAAATAAATAGAAAGGAATTATAGAATGTTCAAATAGAGTGGTAAAATTTTGGTTGGGTGACTGTATAGTACACTGCCTCCTCATGTACCAAATTTCCATAAGAATTTTGATTGGGGTCCTGTACTCTTGTAATGGATCCCCCCTTGTGCTCTTCAGGAGGAGATGCCTCTTCTCCTTGCACATTTTTTGTATTCTCTTCTGAAAAAAGATATATTCATCTCTCCCCCTCAAACTAGTTTGCGAGATGCATCTCATCCAACAAAGTCATGCAAAATTTGGAAGAAGTAATCTCTGGGATTTTCATTTCCATCCTTGTCCCATCTCTCATTATGGTGACATCAATATGAATTTAAGGATTAGTGGAGAAAGATCTTTTGTTTTTTGTGTTTACTTAGTTATTTTTCGATTATAGAAACTATTTTAGTATTATTAAGCTTGGTCATTCATTATGCTAAGTTGAAATGGCGTACATTTCACAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTATTGTAAACTTCTTGCTCTTTAAGTTTCTTCAAAGGGCTATAAATATATATTACAATCCATCTAACAATGCATTTTAACCAATCACCATGTATGCACGTGCAAAGACATATATATTCTTTTGTTTCAATAACATTTCTGTACGCTACTGCTTTTGTTTTCAAGTCATAGTGTGTGCATACGTACGCTAAACAATTTAATGAGTCAGAGTTACACCTTGAACGTACAAGCAGCCATATTTATCATGCCAAGTTGCAGTTCTTTCCACTTTCTGAAAATTGAAATGTAGTTTTTGAATATTTGTGAAGTAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTAGAAAATAAATTCTTAAGAGCTCGTTTAGTTACATTCCTTTCTTGTTCTCTAAAATAGTTTATAATTTTGGTTTCTTATTTCAAAGTTTTTAAAACTTGATGGAGTTTCTATTTTTCTTATTTTTCGCTAATAATTAACCAAAACATTGGTATGACATATATTTGGGATATCTTGTTAAATTTTCAGTTAAAACTTCCACATTTTAAAAGAAAATTTTTAGAGAAAGATAAACAAGAATGATTGCTAATCACAGTCTGTTCTTTTTTAAACTAAAAGGTGAGTGCAAAAGCAAGAATTGTTATAAAATTTTGTTTTTTTTTATTTCTTATGATAAAAGAGCCAGAAATAAAAAGTGAGAAACAAGAAACAAGATCGTAGTATGAGTCTTGGATTGTTCTTTTCTTCTGTAAACAACTAATCCAAGATCACTGAACAATTTGTAGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGTATCTCTCAAGTATTGATGAATTCTGTTATCTTTTACTCAACCCAAGCTCTTTTCATGGTGATTAGTGGTTTTACGGATTTAGCATTTTATCCATAAAATAGAACAATATCAATCAAGAATGGTCCGGGGGCTACGATTTACACACCCAACCTACTTTATGGATAACCTAGATAGCTAATTCGTGCATTTGTGCCTCTTTTTTGGTCGCCTCCTTGACACGATAGTTAATACCATGTCTTTTCTATTTCCTAGCCATGTGGTACCTGCTTCTTGCGTTTGAATGTCTATTTTATAGGTTACTTTCCTTTTGTACTTCATCTCTTACTTATGAAGTCGACTGAACTTAAAAGAAAATCAAGTTGTTTAATGAATGAGTGAGGATTTAGTCATACATATTCAAAGTTTCGAATGGTTTTTTGAGATCCCACATCAGTTGAGGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTATCCCTAGCAGACGTGTTTTAAAACCTTGAGGGGAAGCTCAGAAGGGAAAGCCCAAAGAGGACAATATCTGTTAGTGGTGGGCTTGGGCTTGGGCTTGGGCTATTTCAAATGGTATTAGAGCCAGTCACCGGGCGGTGTGCCAGCGAGGACGTTGGGCCCCCAAGGGGAGTGGATTATGAGATCTCACATTCGATTGGAGAGGGGAACGAAACATTCCTTATATAAGTAGGGAAACCTCTCCCTAGCAGACGTGTTTTAAAACCTTGAGGGGAAGCTTAGAAGGGAAAGTCCAAAGAGGACAATATCGGCTAGCGGTGGACTTGGGTTGTTACAAATGGAATTAAAGCTAGTCACCGGGCGGTGTGCCAGCGAGGACGCTAGGCTCCCAAGGGGGAGTGGATTGTGCGATCCCACATTAGTTGGAGAGGAGAACAAAACATTTCTTATATAGGTGTGGAAACCTCTCCCTAACAAACGCGTTTTAAAACTTTGAGGGGAAGCTCAGAAGGGAAAGCCCAAAAAAGACAATATCTGCCGGTGGTAGGCTTGAGCTGTTACGATAGATATAATACAAAAACTAAAGAGCAGACAGAAAACATGAAGTCTTCCTTCCAGTCGCCTCTTATATGTAATATGAATATAGACACTTTATTTTATCGCATTTGTAGCTATTCCGGATAATCTACACAAAAGATATAATTGGGTTCATAAGCTTAATTTTAAAGATTATTCTCGAAACAAATTCCCTGAAAAACATTGAACTCTACATCATGAGGCAATTTCATATCGTATCTCATTGTTTAATCTCTTGTAGTCCTAATACATAGTACACATTGAAATAATTTTGAGATTTACAACCACATTAGCTATTGAACAATGGTTGCTGCCATAGAATTTAGGCGGGAGAGATGGTTTTTGCGGTGTATCAGTTTAACCATTAACAGAGTGTTGTGATTAAAATTGCAGGTTACCAAATCTTAATTGGGATCCTTCAGTATCTAGCATACACACTCAGCGTCAAACGACTCGGGCATGAAAGACCGAGAAGGTACATTTGGCCCTAATATACGACCATTTAAGTTGGGACGTTTGGAAAATAAATTGAAGTTTTTGGTAAATGACATATTTTTGGTTGGTGTTCTTGGAAATTCAACACTGCTAATTTGATCTGATATGAACAGGGAATGTTCCTAAACTCAATATCCCAATATCGAAATCGAGGCATAGCAGCACGCAACAATGGCATTAGAAATGGAAACGACACACCGTATCGGTACACTTGATGGTGCTTCTACAGTCGAGATGATCGTGTTGCTTGTGTTGAACATTCGCCTAATCGCCATGTCCAATGGAACCTCATGTTTCACTTAGTATTGATGGTAAGCCAGCCACCATTGCTATTAATCCATAGGAATGTAGTCGAATATTCATCGTCATTCAATTGGAAATATATTTGAAAGACATGATTGCAGTAAATTGGGACTCCCTTGAGTGAATTCAAATTAGGCGCTATGAACGTTATGAACATTCCAAGTTACGACATCCCTTTGTAAGGTTCTTTATCTCGGGGTTTAGGAATTAG

mRNA sequence

TAGGCGAAAACCCAAGAACCAAATATCGAACTCGGGAGAATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACACGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGTTACCAAATCTTAATTGGGATCCTTCAGTATCTAGCATACACACTCAGCGTCAAACGACTCGGGCATGAAAGACCGAGAAGGGAATGTTCCTAAACTCAATATCCCAATATCGAAATCGAGGCATAGCAGCACGCAACAATGGCATTAGAAATGGAAACGACACACCGTATCGGTACACTTGATGGTGCTTCTACAGTCGAGATGATCGTGTTGCTTGTGTTGAACATTCGCCTAATCGCCATGTCCAATGGAACCTCATGTTTCACTTAGTATTGATGGTAAGCCAGCCACCATTGCTATTAATCCATAGGAATGTAGTCGAATATTCATCGTCATTCAATTGGAAATATATTTGAAAGACATGATTGCAGTAAATTGGGACTCCCTTGAGTGAATTCAAATTAGGCGCTATGAACGTTATGAACATTCCAAGTTACGACATCCCTTTGTAAGGTTCTTTATCTCGGGGTTTAGGAATTAG

Coding sequence (CDS)

ATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACACGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGTTACCAAATCTTAA

Protein sequence

MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQISTKKVTKS
Homology
BLAST of CmoCh12G003390 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.0e-143
Identity = 256/378 (67.72%), Postives = 306/378 (80.95%), Query Frame = 0

Query: 48  CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
           C    +  PR N AG+R+EEIV+   GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54  CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113

Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
           RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173

Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDTD---ISDDELNSIEILSSRVS-EAPIRPGI 227
           N TGTLVN IL+HCSLP V  +N E   D+D    SDDE    E+ +S  S  A +RPGI
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDE----EMTTSPSSYAASVRPGI 233

Query: 228 VHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDP 287
           VHR+DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP    GRIE+PIGRD 
Sbjct: 234 VHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDS 293

Query: 288 NNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGI 347
           +NRIRM+A+        A++AASRYKVIE  A GG +LVEWRLETGRTHQIRAHAKYMG+
Sbjct: 294 SNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGV 353

Query: 348 PLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIR 407
           PLLGDEVYGGTKSMA+SLL+ R S S   ++++L+S ++RPCLHA+ LGF HP TG+ ++
Sbjct: 354 PLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVK 413

Query: 408 FSCPPPTDFAEILGQLRQ 422
           FSCPPP+D AEI+G LR+
Sbjct: 414 FSCPPPSDLAEIVGLLRR 427

BLAST of CmoCh12G003390 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 7.7e-135
Identity = 253/434 (58.29%), Postives = 304/434 (70.05%), Query Frame = 0

Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           M +  +A     A ++     R R R S    R       RC  +D   +   P  R  +
Sbjct: 1   MATTAAASPPAIATALSALLRRQRRRSS----RCVGASHARCLAADANAEAVAPSRRGGH 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRI--DGISRARVQSSIRAGLVSVNGRVIDKASHNVK 120
            G RLEE V    G+ R+D+WIS R+   G+SRAR+Q+SIRAGLV VNGR + K SH VK
Sbjct: 61  GGTRLEEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVK 120

Query: 121 AGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAIL 180
            GD ++CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL
Sbjct: 121 GGDIVSCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAIL 180

Query: 181 NHCSLPMVTTTNEELLSD--TDISDDELNSIEI-------LSSRVSEAPIRPGIVHRIDK 240
           +HC +   T      + D   D SDD+++  +I       +SS V EA +RPGIVHR+DK
Sbjct: 181 HHCKISTFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDK 240

Query: 241 GTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRM 300
           GTSGLLVVAKDEH+HA L+ QFK H+I RVYISLTCG P   +GRIEVPI RDPNNRIRM
Sbjct: 241 GTSGLLVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRM 300

Query: 301 SAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDE 360
            A   + +   A++AASRYKV E  A GG +LVEWRLETGRTHQIRAHAKY+GIPLLGDE
Sbjct: 301 IATPGSGHR-YARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDE 360

Query: 361 VYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPP 420
            YGGTKSMA+SLLR RT S  H  L  ++S ++RPCLHA  LGF HPH+GK + FSCPPP
Sbjct: 361 TYGGTKSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKHPHSGKILEFSCPPP 420

Query: 421 TDFAEILGQLRQIS 424
            DF E+L +L Q++
Sbjct: 421 DDFTEVLNELHQVT 429

BLAST of CmoCh12G003390 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 1.0e-54
Identity = 143/350 (40.86%), Postives = 194/350 (55.43%), Query Frame = 0

Query: 77  RLDSWISCRIDGISRARVQSSIRAGLVS-VNGRVIDKASHNVKAGDKINCTISELQPLRA 136
           RLD  ++  I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 137 EPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLS 196
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+             
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA------------- 135

Query: 197 DTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHS 256
                  +L+ I  ++        RPGIVHRIDK TSGLLVVAK + AHA L+AQF  HS
Sbjct: 136 ------GKLSGIGGVA--------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHS 195

Query: 257 IERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLA 316
           I R Y ++  G  PS  G ++ P+ R   NR +M+ VS    +   K A + Y++I  L 
Sbjct: 196 INRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIVS----DGRGKRAVTHYRMITPLK 255

Query: 317 QGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLM 376
               SL+E RLETGRTHQ+R H   +G  LLGD VYG +K               H  L+
Sbjct: 256 NA--SLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSK-------------KAHHALL 315

Query: 377 QLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQISTK 426
           Q + + +R  LHA  LGFIHP +GK + F    P D  ++L ++  IS +
Sbjct: 316 QSL-AFQRQALHAAHLGFIHPISGKQVDFDAEMPQDM-QLLFKMLMISNR 316

BLAST of CmoCh12G003390 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-54
Identity = 135/345 (39.13%), Postives = 186/345 (53.91%), Query Frame = 0

Query: 77  RLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAE 136
           R+D +++   +  SR +VQ  ++ G V VNG  + KA++ ++ GD++  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 137 PEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD 196
            E + LDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC               
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHC--------------- 135

Query: 197 TDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSI 256
           TD+S   +N +           +RPGIVHRIDK TSGLL+VAK++ AH  L  Q    ++
Sbjct: 136 TDLSG--INGV-----------MRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTV 195

Query: 257 ERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQ 316
            R Y ++  G+     G I+ PIGRD  +R  M+ V+R   N     A + + V+E    
Sbjct: 196 TRKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMT-VTRDGKN-----AVTHFHVLERFQD 255

Query: 317 GGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQ 376
             F+LVE +LETGRTHQIR H KY+G PL GD  YG  K++                   
Sbjct: 256 --FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI------------------- 301

Query: 377 LVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQ 422
                    LHA  LGF HP TG+ + F  P P D AE++  LR+
Sbjct: 316 ---DFNGQALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRK 301

BLAST of CmoCh12G003390 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.1e-54
Identity = 132/349 (37.82%), Postives = 197/349 (56.45%), Query Frame = 0

Query: 76  VRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRA 135
           +R+D +++ +++ ++R +VQ++I  G V VNG+ + K+++ VK+ D I+ T         
Sbjct: 12  MRIDRYLTQQVENMTRNKVQAAIEEGRVLVNGKPV-KSNYRVKSCDHIHLTFLRPPAPEL 71

Query: 136 EPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLS 195
            PEDI + I+YED+ ++V++K A MVVHPA GN TGTL NAIL+H               
Sbjct: 72  APEDIPITILYEDDDLMVIDKEAGMVVHPAFGNWTGTLANAILHHLGK------------ 131

Query: 196 DTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHS 255
                 DEL+  E          +RPGIVHR+DK TSGL+++AK+  A   L+ QF    
Sbjct: 132 ----DADELDKTE----------MRPGIVHRLDKDTSGLIIIAKNPTALHKLARQFANRQ 191

Query: 256 IERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLA 315
           +E+VY ++  GVP + +G I+  IGR   NR  M+  +     +  K+A + Y+V+E+L 
Sbjct: 192 VEKVYKAIVWGVPKAESGTIKTNIGRSHKNRKVMA--NFPYEGAEGKHAITDYQVVEDL- 251

Query: 316 QGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLM 375
            G FSL++  L TGRTHQIR H +++G  +LGDE YGG         RT   S       
Sbjct: 252 -GYFSLMDVTLHTGRTHQIRVHLQHLGHSILGDETYGGATP------RTLPFSKSEPFTR 311

Query: 376 QLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQIST 425
            L+  + R  LHA TL F  P TG+ + FS P P D   +L ++R + T
Sbjct: 312 NLLELMSRQALHAQTLRFRQPTTGEPLSFSAPLPEDMELVLEKIRTVMT 323

BLAST of CmoCh12G003390 vs. ExPASy TrEMBL
Match: A0A6J1GIW0 (Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111454672 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.6e-244
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY
Sbjct: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG
Sbjct: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420

Query: 421 QISTKKVTKS 431
           QISTKKVTKS
Sbjct: 421 QISTKKVTKS 430

BLAST of CmoCh12G003390 vs. ExPASy TrEMBL
Match: A0A6J1KJA7 (Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111496217 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.8e-235
Identity = 418/430 (97.21%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MIS+CSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHS RCSGSDECG ETLPEP TNY
Sbjct: 1   MISVCSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSARCSGSDECG-ETLPEPTTNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIV+T SGKVR+DSWISCRIDGISRARVQSSIRAGLVSVNGRVI+KASHNVKAG
Sbjct: 61  AGMRLEEIVKTNSGKVRIDSWISCRIDGISRARVQSSIRAGLVSVNGRVIEKASHNVKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DKINCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMVTTTNEELLSD DISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS+
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSH 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSS HSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSSHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420

Query: 421 QISTKKVTKS 431
           QISTKKVTKS
Sbjct: 421 QISTKKVTKS 429

BLAST of CmoCh12G003390 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 375/430 (87.21%), Postives = 396/430 (92.09%), Query Frame = 0

Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MIS+CS RAIQAAPSIQLFFPRNRGRI+ +V RIATNHS RCS  DECG E   E  TNY
Sbjct: 1   MISVCSVRAIQAAPSIQLFFPRNRGRITSNVHRIATNHSTRCSDYDECG-EAHSERITNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIV+  SGK+RLDSWISCRI+GISRARVQSSIRAGLV+VNGRVIDK SHN+KAG
Sbjct: 61  AGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D INCTISELQPLRA+PEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMV T+N E LSD DISDDE +S EILSSR SEAPIRPGIVHR+DKGTSGLLVVAKD
Sbjct: 181 CSLPMVATSNGE-LSDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPI RDPNNRIRMSAV RTN N +
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNPH 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIE LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+S
Sbjct: 301 AKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSSCHSQLMQLVS+LERPCLHALTLGFIHP+TG+NIRFSCPPPTDFA+I  QL 
Sbjct: 361 LLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQLC 420

Query: 421 QISTKKVTKS 431
           +I TKKVT+S
Sbjct: 421 EIGTKKVTES 428

BLAST of CmoCh12G003390 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.1e-196
Identity = 358/431 (83.06%), Postives = 380/431 (88.17%), Query Frame = 0

Query: 1   MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+P SI  FFPRNR RI  +VLR + N S RCS  DE   E   E   N
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV+T+  E  SD+D+SDDE +SIE+  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A   T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420

Query: 421 RQISTKKVTKS 431
           R+I TKKVTKS
Sbjct: 421 REIGTKKVTKS 429

BLAST of CmoCh12G003390 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 6.9e-195
Identity = 358/433 (82.68%), Postives = 380/433 (87.76%), Query Frame = 0

Query: 1   MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+P SI  FFPRNR RI  +VLR + N S RCS  DE   E   E   N
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV+T+  E  SD+D+SDDE +SIE+  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A   T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420

Query: 421 RQISTKK--VTKS 431
           R+I TKK  VTKS
Sbjct: 421 REIGTKKLQVTKS 431

BLAST of CmoCh12G003390 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 510.4 bits (1313), Expect = 1.4e-144
Identity = 256/378 (67.72%), Postives = 306/378 (80.95%), Query Frame = 0

Query: 48  CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
           C    +  PR N AG+R+EEIV+   GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54  CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113

Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
           RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173

Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDTD---ISDDELNSIEILSSRVS-EAPIRPGI 227
           N TGTLVN IL+HCSLP V  +N E   D+D    SDDE    E+ +S  S  A +RPGI
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDE----EMTTSPSSYAASVRPGI 233

Query: 228 VHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDP 287
           VHR+DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP    GRIE+PIGRD 
Sbjct: 234 VHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDS 293

Query: 288 NNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGI 347
           +NRIRM+A+        A++AASRYKVIE  A GG +LVEWRLETGRTHQIRAHAKYMG+
Sbjct: 294 SNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGV 353

Query: 348 PLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIR 407
           PLLGDEVYGGTKSMA+SLL+ R S S   ++++L+S ++RPCLHA+ LGF HP TG+ ++
Sbjct: 354 PLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVK 413

Query: 408 FSCPPPTDFAEILGQLRQ 422
           FSCPPP+D AEI+G LR+
Sbjct: 414 FSCPPPSDLAEIVGLLRR 427

BLAST of CmoCh12G003390 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 340.1 bits (871), Expect = 2.6e-93
Identity = 177/264 (67.05%), Postives = 209/264 (79.17%), Query Frame = 0

Query: 48  CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
           C    +  PR N AG+R+EEIV+   GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54  CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113

Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
           RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173

Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDTD---ISDDELNSIEILSSRVS-EAPIRPGI 227
           N TGTLVN IL+HCSLP V  +N E   D+D    SDDE    E+ +S  S  A +RPGI
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDE----EMTTSPSSYAASVRPGI 233

Query: 228 VHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDP 287
           VHR+DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP    GRIE+PIGRD 
Sbjct: 234 VHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDS 293

Query: 288 NNRIRMSAVSRTNNNSNAKYAASR 308
           +NRIRM+A+        A++AASR
Sbjct: 294 SNRIRMAAIPGGVRGGRARHAASR 313

BLAST of CmoCh12G003390 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 76.6 bits (187), Expect = 5.3e-14
Identity = 63/226 (27.88%), Postives = 108/226 (47.79%), Query Frame = 0

Query: 144 IVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDTDISDDE 203
           ++Y+D  ++V+NKP  + V    G  T                             S DE
Sbjct: 185 VLYKDPAIIVLNKPHGLAVQGGSGIKT-----------------------------SIDE 244

Query: 204 LNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ---------- 263
           L +   L    SE+   P +VHR+D+  SGLLV+A+ + A   L + F++          
Sbjct: 245 L-AASCLKFDKSES---PRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGV 304

Query: 264 ----HSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS--NAKYAASR 323
                S++R Y++L  G PP   G+I  P+ +   +  +   ++  +N    + ++A + 
Sbjct: 305 KKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITE 364

Query: 324 YKVIENLAQGGFSLVEWRLETGRTHQIRAH-AKYMGIPLLGDEVYG 353
           Y+VIE+ +  G++ +E R  TGR HQ+R H A+ +G P++GD  YG
Sbjct: 365 YRVIES-SPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 376

BLAST of CmoCh12G003390 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.9e-14
Identity = 75/274 (27.37%), Postives = 123/274 (44.89%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIY-LDIVYEDEHVLVVNK 157
           I+ G + ++G V+   +  +++G K+   +    P + EP+  Y L+++YED+ ++ +NK
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWK-EPDTPYSLEVLYEDDDLIALNK 115

Query: 158 PAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSE 217
           P+ + V P      G L            V T  +      D           + SR S 
Sbjct: 116 PSGLQVLP------GGLFQQ-------RTVLTQLQWCFGKND---------SYIGSRESP 175

Query: 218 APIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ-----------------HSIERV 277
            P+    VHR+ +GTSG+L+ AK + A   L+A F +                   + ++
Sbjct: 176 HPVP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKI 235

Query: 278 YISLTCGVPPSTAGRIEVPIG--RDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQG 337
           Y +L  G+       I+ PIG  R P     +   S        K A S+  V+E   + 
Sbjct: 236 YRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREK 295

Query: 338 GFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 352
             SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 296 NCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

BLAST of CmoCh12G003390 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-11
Identity = 72/268 (26.87%), Postives = 118/268 (44.03%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIY-LDIVYEDEHVLVVNK 157
           I+ G + ++G V+   +  +++G K+   +    P + EP+  Y L+++YED+ ++ +NK
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWK-EPDTPYSLEVLYEDDDLIALNK 115

Query: 158 PAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSE 217
           P+ + V P      G L            V T  +      D           + SR S 
Sbjct: 116 PSGLQVLP------GGLFQQ-------RTVLTQLQWCFGKND---------SYIGSRESP 175

Query: 218 APIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ-----------------HSIERV 277
            P+    VHR+ +GTSG+L+ AK + A   L+A F +                   + ++
Sbjct: 176 HPVP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKI 235

Query: 278 YISLTCGVPPSTAGRIEVPIG--RDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQG 337
           Y +L  G+       I+ PIG  R P     +   S        K A S+  V+E   + 
Sbjct: 236 YRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREK 289

Query: 338 GFSLVEWRLETGRTHQIRAHAKYMGIPL 346
             SLV+  +++GR HQIR H  YMG PL
Sbjct: 296 NCSLVKVEIQSGRPHQIRIHLAYMGHPL 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ECD02.0e-14367.72RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5Z8P27.7e-13558.29RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
P505131.0e-5440.86Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... [more]
Q454801.3e-5439.13Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... [more]
O503105.1e-5437.82Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st... [more]
Match NameE-valueIdentityDescription
A0A6J1GIW03.6e-244100.00Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111454672 PE=3 SV=1[more]
A0A6J1KJA71.8e-23597.21Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111496217 PE=3 SV=1[more]
A0A6J1CMS31.9e-20887.21Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1[more]
A0A1S3BD562.1e-19683.06Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BCE46.9e-19582.68Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G76050.21.4e-14467.72Pseudouridine synthase family protein [more]
AT1G76050.12.6e-9367.05Pseudouridine synthase family protein [more]
AT3G19440.15.3e-1427.88Pseudouridine synthase family protein [more]
AT3G52260.26.9e-1427.37Pseudouridine synthase family protein [more]
AT3G52260.11.9e-1126.87Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 76..138
e-value: 0.0013
score: 28.0
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 77..123
e-value: 6.3E-8
score: 32.2
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 76..155
e-value: 3.10347E-12
score: 59.5706
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 75..419
e-value: 4.6E-75
score: 250.6
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 61..131
e-value: 1.7E-17
score: 64.7
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 150..338
e-value: 2.1E-33
score: 115.7
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 151..393
e-value: 9.78257E-66
score: 206.42
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 133..408
e-value: 7.6E-69
score: 233.6
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 71..421
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 71..421
NoneNo IPR availablePROSITEPS50889S4coord: 76..149
score: 12.041144
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 75..163
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 224..238
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 136..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G003390.1CmoCh12G003390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding