CmoCh12G002200 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G002200
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	tRNA (guanine-N(7)-)-methyltransferase
Location	Cmo_Chr12: 1457297 .. 1458055 (-)
RNA-Seq Expression	CmoCh12G002200
Synteny	CmoCh12G002200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGATCATGATGCTAATTCAACTATCAGCAAGTCAACGGGCCTGCCTCGGAAGCGTTTCTACCGTGCTAGAGCGCACAGCAACCCTCTGAGCGACTCTCACTTCCCAATACCAATCTGCCCCAGTGAAGTTGACTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGATCAGCTACATAGATCCAAAAAGATTGAATTTGCTGATATTGGCTGTGGTTTTGGAGGTCTTCTGATCAGCCTTTCGACCCTTTTCCCTGAAACCTTGATGATCGGAATGGAACTCCGAGATAAAGTAACCGAATACGTTAAGGAACGGATCTTAGCGTTGAGAGTAGCAAATCCTGGCCAGTACCAAAACGTTTCGGTAGTTCGAACAAATTCCATGAAATACATCCCAAACTACTTCGAAAAAGCTCAACTATCAAAAATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTTATCAGTCCACATTTGCTCGACGAGTATGCATATACCCTGAGAGTCGGAGGGATTATATACACAATTACAGACGTGGAAGAGCTTGGAGAATGGATGAAATCTTGTCTAGAAAACCACCCGTTGTTCGAAGCTCTAACAGCGGAAGAGCTCGAGGTAGATCCAGCCGTAAAGCTGTTGAGTTCTGCAACTGAGGAAGGGCAGAAGGTTGCTAGAAATGAGGGACAGACATTTCAGGCAGTTTACAGACGTATTAGTGCAGCACCATGA

mRNA sequence

ATGTTGGATCATGATGCTAATTCAACTATCAGCAAGTCAACGGGCCTGCCTCGGAAGCGTTTCTACCGTGCTAGAGCGCACAGCAACCCTCTGAGCGACTCTCACTTCCCAATACCAATCTGCCCCAGTGAAGTTGACTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGATCAGCTACATAGATCCAAAAAGATTGAATTTGCTGATATTGGCTGTGGTTTTGGAGGTCTTCTGATCAGCCTTTCGACCCTTTTCCCTGAAACCTTGATGATCGGAATGGAACTCCGAGATAAAGTAACCGAATACGTTAAGGAACGGATCTTAGCGTTGAGAGTAGCAAATCCTGGCCAGTACCAAAACGTTTCGGTAGTTCGAACAAATTCCATGAAATACATCCCAAACTACTTCGAAAAAGCTCAACTATCAAAAATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTTATCAGTCCACATTTGCTCGACGAGTATGCATATACCCTGAGAGTCGGAGGGATTATATACACAATTACAGACGTGGAAGAGCTTGGAGAATGGATGAAATCTTGTCTAGAAAACCACCCGTTGTTCGAAGCTCTAACAGCGGAAGAGCTCGAGGTAGATCCAGCCGTAAAGCTGTTGAGTTCTGCAACTGAGGAAGGGCAGAAGGTTGCTAGAAATGAGGGACAGACATTTCAGGCAGTTTACAGACGTATTAGTGCAGCACCATGA

Coding sequence (CDS)

ATGTTGGATCATGATGCTAATTCAACTATCAGCAAGTCAACGGGCCTGCCTCGGAAGCGTTTCTACCGTGCTAGAGCGCACAGCAACCCTCTGAGCGACTCTCACTTCCCAATACCAATCTGCCCCAGTGAAGTTGACTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGATCAGCTACATAGATCCAAAAAGATTGAATTTGCTGATATTGGCTGTGGTTTTGGAGGTCTTCTGATCAGCCTTTCGACCCTTTTCCCTGAAACCTTGATGATCGGAATGGAACTCCGAGATAAAGTAACCGAATACGTTAAGGAACGGATCTTAGCGTTGAGAGTAGCAAATCCTGGCCAGTACCAAAACGTTTCGGTAGTTCGAACAAATTCCATGAAATACATCCCAAACTACTTCGAAAAAGCTCAACTATCAAAAATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTTATCAGTCCACATTTGCTCGACGAGTATGCATATACCCTGAGAGTCGGAGGGATTATATACACAATTACAGACGTGGAAGAGCTTGGAGAATGGATGAAATCTTGTCTAGAAAACCACCCGTTGTTCGAAGCTCTAACAGCGGAAGAGCTCGAGGTAGATCCAGCCGTAAAGCTGTTGAGTTCTGCAACTGAGGAAGGGCAGAAGGTTGCTAGAAATGAGGGACAGACATTTCAGGCAGTTTACAGACGTATTAGTGCAGCACCATGA

Protein sequence

MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYRRISAAP

Homology

[Close]

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot
Match: Q8GXB7 (tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24840 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 4.5e-111
Identity = 198/243 (81.48%), Postives = 219/243 (90.12%), Query Frame = 0


Query: 8   STISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRSKKI 67
           +T SKSTGLPRKRFYRARAHSNPLSDSHFPIPI P+ VDYSLH+P+   ++++    KK+
Sbjct: 8   TTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKF--IKKV 67

Query: 68  EFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILAL-RVANPGQYQNVSVV 127
           EFADIGCGFGGLLISL+TLFP+TLMIGMELRDKVTEYVKERILAL R ++ GQY+N+SVV
Sbjct: 68  EFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVV 127

Query: 128 RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTI 187
           RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS HLLDEYAY LR GGIIYTI
Sbjct: 128 RTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTI 187

Query: 188 TDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYR 247
           TDVEELGEWMKSCLE HP+FE+LT EEL  DP V+LL SATEEGQKVARN GQTF+AV+R
Sbjct: 188 TDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFR 247

Query: 248 RIS 250
           RI+
Sbjct: 248 RIA 248

[Close]

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot
Match: A2YB34 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22311 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 6.1e-108
Identity = 191/239 (79.92%), Postives = 207/239 (86.61%), Query Frame = 0


Query: 16  LPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRS-----KKIEFA 75
           LPRKRFYRARAHSNPLSDSHFPIPI P EVD S HYP+ FPS +   R       +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNVSVVRTNS 135
           D+GCGFGGLL+ LSTLFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y N+SVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 250
           ELGEWM+SCLE HPLFEA+  EE + DP VKLLS+ATEEGQKVARN GQTFQA++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

[Close]

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot
Match: Q67V78 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0236900 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 6.1e-108
Identity = 191/239 (79.92%), Postives = 207/239 (86.61%), Query Frame = 0


Query: 16  LPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRS-----KKIEFA 75
           LPRKRFYRARAHSNPLSDSHFPIPI P EVD S HYP+ FPS +   R       +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNVSVVRTNS 135
           D+GCGFGGLL+ LSTLFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y N+SVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 250
           ELGEWM+SCLE HPLFEA+  EE + DP VKLLS+ATEEGQKVARN GQTFQA++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

[Close]

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot
Match: B6SHG7 (tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 8.3e-105
Identity = 184/239 (76.99%), Postives = 205/239 (85.77%), Query Frame = 0


Query: 16  LPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSN-----DQLHRSKKIEFA 75
           LPRKRFYRARAHSNPLSDSHFP+PI P EVD S HYP+ FP++     ++    ++I FA
Sbjct: 13  LPRKRFYRARAHSNPLSDSHFPVPISPEEVDLSQHYPRYFPADKGSDGEEAAAPQQIRFA 72

Query: 76  DIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNVSVVRTNS 135
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR ANPGQY N+SVVRTNS
Sbjct: 73  DVGCGFGGLLVGLSPLFPDTLMIGMELRDKVTEYVKERILALRGANPGQYDNISVVRTNS 132

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTITDVE 195
           MKYIPNYF KAQL+KMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 133 MKYIPNYFRKAQLTKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 192

Query: 196 ELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 250
           ELGEWM+SCLE HPLFE +  EE++ DP  KLLS+ATEE QKVARN GQTF A++RRIS
Sbjct: 193 ELGEWMRSCLEKHPLFETVPEEEIKADPVFKLLSTATEESQKVARNGGQTFYAIFRRIS 251

[Close]

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot
Match: A9UMM1 (tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1-B PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.5e-77
Identity = 133/236 (56.36%), Postives = 182/236 (77.12%), Query Frame = 0


Query: 13  STGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRSKKIEFADI 72
           S  +P+KR+YR RAHSNP++D  F  P+CP ++D+S  YPQ FP  ++     ++EFADI
Sbjct: 2   SVCMPQKRYYRQRAHSNPMADHTFQYPVCPEQMDWSPLYPQYFPQQEEA-GGAQVEFADI 61

Query: 73  GCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNVSVVRTNSMK 132
           GCG+GGLL+ LS LFP+ L++G+E+R KV++YV++RI +LRVA PG+YQN++ +R+N+MK
Sbjct: 62  GCGYGGLLVQLSLLFPQQLILGLEIRVKVSDYVQDRIRSLRVAEPGRYQNIACLRSNAMK 121

Query: 133 YIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTITDVEEL 192
           Y+PN+F K QLSKMFFLFPDPHFK+  H+ R+ISP LL EYAYTLR+GG++YT TDVEE+
Sbjct: 122 YLPNFFRKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTNTDVEEV 181

Query: 193 GEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYRRI 249
            EW+     +HPLF  +T E+L  D  V  L + TEEG+KV RN G+ F AV+RR+
Sbjct: 182 HEWIVQHFSDHPLFSRVTEEQLADDIIVGHLGTCTEEGKKVQRNGGKNFLAVFRRV 236

[Close]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL
Match: A0A6J1GGH7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454015 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.0e-141
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60
           MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ
Sbjct: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
           LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180
           QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FQAVYRRISAAP 253
           FQAVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

[Close]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL
Match: A0A6J1KTN7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496303 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.4e-139
Identity = 248/252 (98.41%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60
           ML+HDAN TISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
           LHR KKIEFADIGCGFGGLLISLST+FPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRPKKIEFADIGCGFGGLLISLSTIFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180
           QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FQAVYRRISAAP 253
           FQAVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

[Close]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL
Match: A0A6J1BTJ1 (tRNA (guanine-N(7)-)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005201 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 236/252 (93.65%), Postives = 244/252 (96.83%), Query Frame = 0


Query: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60
           ML+HDAN TISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60

Query: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             RSKK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LRVG
Sbjct: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180

Query: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240

Query: 241 FQAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FRAVYRRISAAP 252

[Close]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL
Match: A0A1S4DVC5 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103488206 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 9.7e-133
Identity = 236/252 (93.65%), Postives = 245/252 (97.22%), Query Frame = 0


Query: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60
           ML+++AN TISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQLFPSNDQ
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             ++KKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180
           QN+SV RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAY LRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALT+EELE DPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FQAVYRRISAAP 253
           FQAVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

[Close]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL
Match: A0A5A7VCU7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003450 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 9.7e-133
Identity = 236/252 (93.65%), Postives = 245/252 (97.22%), Query Frame = 0


Query: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60
           ML+++AN TISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQLFPSNDQ
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             ++KKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180
           QN+SV RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAY LRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALT+EELE DPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FQAVYRRISAAP 253
           FQAVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

[Close]

BLAST of CmoCh12G002200 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 402.1 bits (1032), Expect = 3.2e-112
Identity = 198/243 (81.48%), Postives = 219/243 (90.12%), Query Frame = 0


Query: 8   STISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQLHRSKKI 67
           +T SKSTGLPRKRFYRARAHSNPLSDSHFPIPI P+ VDYSLH+P+   ++++    KK+
Sbjct: 8   TTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKF--IKKV 67

Query: 68  EFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILAL-RVANPGQYQNVSVV 127
           EFADIGCGFGGLLISL+TLFP+TLMIGMELRDKVTEYVKERILAL R ++ GQY+N+SVV
Sbjct: 68  EFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVV 127

Query: 128 RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGGIIYTI 187
           RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS HLLDEYAY LR GGIIYTI
Sbjct: 128 RTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTI 187

Query: 188 TDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQTFQAVYR 247
           TDVEELGEWMKSCLE HP+FE+LT EEL  DP V+LL SATEEGQKVARN GQTF+AV+R
Sbjct: 188 TDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFR 247

Query: 248 RIS 250
           RI+
Sbjct: 248 RIA 248

[Close]

BLAST of CmoCh12G002200 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-08
Identity = 52/200 (26.00%), Postives = 89/200 (44.50%), Query Frame = 0


Query: 7   NSTISKSTGLPRKRFYRARAHSNPLSDSHF-PIPICPSEVDYSLHYPQLFPSNDQLHRSK 66
           N  IS+  G       R R H NPLS S   P P+               P  D++++  
Sbjct: 84  NHKISEEVG-----HVRIRQHVNPLSSSFSKPAPV---------------PVWDEVYKDP 143

Query: 67  KIE-FADIGCGFGGLLISLSTLFPETL-MIGMELRDKVTEYVKERILALRVANPGQYQNV 126
            +    DIG G G  L+  +    E+   +G+E+R K+ +     +  L ++      NV
Sbjct: 144 SLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS------NV 203

Query: 127 SVVRTNSMKYIPNYFEK--AQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVGG 186
             +  N+M    +        L  +  L PDPHFK+++ +RRV+   L++     L+ GG
Sbjct: 204 HFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKPGG 257

Query: 187 IIYTITDVEELGEWMKSCLE 202
            I+  +DV ++ + M+  L+
Sbjct: 264 KIFVQSDVLDVAQDMRDQLD 257

The following BLAST results are available for this feature:

BLAST of CmoCh12G002200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXB7	4.5e-111	81.48	tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24...	[more]
A2YB34	6.1e-108	79.92	tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN...	[more]
Q67V78	6.1e-108	79.92	tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
B6SHG7	8.3e-105	76.99	tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1	[more]
A9UMM1	2.5e-77	56.36	tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1...	[more]

BLAST of CmoCh12G002200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GGH7	2.0e-141	100.00	tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1KTN7	1.4e-139	98.41	tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114963...	[more]
A0A6J1BTJ1	5.7e-133	93.65	tRNA (guanine-N(7)-)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A1S4DVC5	9.7e-133	93.65	tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103488206 P...	[more]
A0A5A7VCU7	9.7e-133	93.65	tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of CmoCh12G002200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24840.1	3.2e-112	81.48	tRNA (guanine-N-7) methyltransferase	[more]
AT5G17660.1	2.5e-08	26.00	tRNA (guanine-N-7) methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	TIGRFAM	TIGR00091	TIGR00091	coord: 64..248 e-value: 8.0E-43 score: 144.0
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PFAM	PF02390	Methyltransf_4	coord: 67..242 e-value: 8.6E-52 score: 175.1
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PANTHER	PTHR23417	3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE	coord: 11..249
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PROSITE	PS51625	SAM_MT_TRMB	coord: 19..247 score: 58.19582
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 45..247 e-value: 2.7E-81 score: 274.1
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 69..186 e-value: 4.17908E-4 score: 37.0243
IPR025763	tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote	PANTHER	PTHR23417:SF16	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE	coord: 11..249
IPR025763	tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote	HAMAP	MF_03055	tRNA_methyltr_TrmB_euk	coord: 16..248 score: 44.732193
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 61..214

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G002200.1	CmoCh12G002200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106004	tRNA (guanine-N7)-methylation
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005634	nucleus
cellular_component	GO:0043527	tRNA methyltransferase complex
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity