CmoCh12G001850 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G001850
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPoly [ADP-ribose] polymerase
LocationCmo_Chr12: 1210695 .. 1212579 (+)
RNA-Seq ExpressionCmoCh12G001850
SyntenyCmoCh12G001850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGATTTCGATGGGTGACTGGGAAACGGATACATATGGCAGCACGCTAAGTTTCATCGCCTCGATTCTGCTGGTTCTTTCGAAGCGAAATTTCAGTGCCGGCTTTCGATCCCAGAGACTGGTAGAATCCATGGCCTCCTCTAAGCTTCTGATGCATAAGCAATTCAGGAAGAAGATGGTGGAATCGGTTGCGGTTCGGGCTCCTCCGAAGCCTTGTTCGAGAAACCCTATCGATTCCTCCTCTTGTCATCACTTATCCGATTCTTCGCAGTCTTTGATTCAAAACTACTCCAATTTCAAGGCCAGTGGTTTGCCTTCTCGTTTCATGTTCTATCAGGATAGGTTGTGGGTTGATTTCGATAATCGAGTGATTGGAATATTGCGAGCGGGTTTCTCCGACCGGAATCCGGCGATTGAACTGGAAATCGATGGATCGACGTATCTGTTTGATATGTATAGGATGTTACAGATTGATTTGGAAACTGGTAGACAGAGATCGGTTGCTTGGATCGATGAAAATGGGAAGTGTTTTTTCCCCAAAGTCTTTATCGGCGATGAGAGTGAGGGAAATACTCGGAGTTTGTCGAATCCGAAAATTGAAATTGAGATTAAGATTGATCGGAAGTCCGGGAAACGAAAGAGGGAAGCGATGGAGATGGAGGAGGAAGAAAATGAAGTGAATTCCAGCAATGAACATGTGGAAGTGAAGCCATTGAAAATACCGCGTGTTGTTGCGAATGATTCAGAAGCTCATGTATGGCCGAAGGCGAAGGCGCTGAGTGAAGGAGATAGTGCGTACACGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCCAGTTCTGCAATCACCTCAATTCATCAGTGCACCCGCACGGGGCCATTGGAAAAAGCTCGCCTCGATGTGTTTCAAAAGCAGAATGAGATTACAACGGCTGCCCGTGGTGTGTCCAATATGGTTTATGCATGGTATGGAGCTTCTGCAACAAGTTTGGCGGGAATTTTGGCTCATGGATTTGGGGAGCCTCTTCAGGTGCCTGCCGCCGATACTCATGGAATTGGAGTTTACTTATCTCCATTGGGCTTGCCTCATTTATGGTAATTTGGCTTTCATTTTCGTCTAGCTACTTCTAGTTTATGATTATGTGTTCTCACCATATTCAAATGCACAATTAACTGTGATTCCCAGTTCTACGCTATCAGAGGTCGATGAGGATGGTATAAAACATATGGTTTTATGTCGAGTAATATTGGGTAATGTTGAAAAGGTTGAAGCAGGATCTCAGCAATCCCATCCGTCGAGTACAGAGTTTGACACTGGTGTGGATGATCCAACATGTCCTAAACGGTATATTGTCTGGTGTAGTAATATGAACAGACACATTTTACCAGAGTACATAGTGAGTTTCAGATCAAATTATCGTTTGCCAGGTAAATATTCTTATTGATTGGGTTTATTTGCTTTATCCTCTTCGCTTTTTTAGTTTCGTTGATGATTCTTTTTTCCAGGTAAGTTGGGGGAATCGACAGATACGAAGTATTCCCTCGTGAAATTACTTTCGAAGATGAAGAATTCGCTGCCTACCTCTAAAGTTCAAGAGGTTGCGACATTGTTTCAGACATTTAAGGTGAGTCTTTGTTGTGTAACTTTTAAAAAGATGCACTCCTATTCACTAAAAGAAAAAATGAAAGGGTAAGAGAAGAATGTGAAATAGGACTAAGTAATTGAAGATAATATGATGCTAATTTAGCAACACGCATTTGATCATTACAGGCAGGAAAATTGGGAAAAGATGTTCTTGTTAAAAGGCTGCGATCAATTGCTGGAGATGACATGCTTCTATCCTTAATTCGTGAAATTCGAGAATGTCAATGA

mRNA sequence

TTTGATTTCGATGGGTGACTGGGAAACGGATACATATGGCAGCACGCTAAGTTTCATCGCCTCGATTCTGCTGGTTCTTTCGAAGCGAAATTTCAGTGCCGGCTTTCGATCCCAGAGACTGGTAGAATCCATGGCCTCCTCTAAGCTTCTGATGCATAAGCAATTCAGGAAGAAGATGGTGGAATCGGTTGCGGTTCGGGCTCCTCCGAAGCCTTGTTCGAGAAACCCTATCGATTCCTCCTCTTGTCATCACTTATCCGATTCTTCGCAGTCTTTGATTCAAAACTACTCCAATTTCAAGGCCAGTGGTTTGCCTTCTCGTTTCATGTTCTATCAGGATAGGTTGTGGGTTGATTTCGATAATCGAGTGATTGGAATATTGCGAGCGGGTTTCTCCGACCGGAATCCGGCGATTGAACTGGAAATCGATGGATCGACGTATCTGTTTGATATGTATAGGATGTTACAGATTGATTTGGAAACTGGTAGACAGAGATCGGTTGCTTGGATCGATGAAAATGGGAAGTGTTTTTTCCCCAAAGTCTTTATCGGCGATGAGAGTGAGGGAAATACTCGGAGTTTGTCGAATCCGAAAATTGAAATTGAGATTAAGATTGATCGGAAGTCCGGGAAACGAAAGAGGGAAGCGATGGAGATGGAGGAGGAAGAAAATGAAGTGAATTCCAGCAATGAACATGTGGAAGTGAAGCCATTGAAAATACCGCGTGTTGTTGCGAATGATTCAGAAGCTCATGTATGGCCGAAGGCGAAGGCGCTGAGTGAAGGAGATAGTGCGTACACGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCCAGTTCTGCAATCACCTCAATTCATCAGTGCACCCGCACGGGGCCATTGGAAAAAGCTCGCCTCGATGTGTTTCAAAAGCAGAATGAGATTACAACGGCTGCCCGTGGTGTGTCCAATATGGTTTATGCATGGTATGGAGCTTCTGCAACAAGTTTGGCGGGAATTTTGGCTCATGGATTTGGGGAGCCTCTTCAGGTGCCTGCCGCCGATACTCATGGAATTGGAGTTTACTTATCTCCATTGGGCTTGCCTCATTTATGTTCTACGCTATCAGAGGTCGATGAGGATGGTATAAAACATATGGTTTTATGTCGAGTAATATTGGGTAATGTTGAAAAGGTTGAAGCAGGATCTCAGCAATCCCATCCGTCGAGTACAGAGTTTGACACTGGTGTGGATGATCCAACATGTCCTAAACGGTATATTGTCTGGTGTAGTAATATGAACAGACACATTTTACCAGAGTACATAGTGAGTTTCAGATCAAATTATCGTTTGCCAGGTAAGTTGGGGGAATCGACAGATACGAAGTATTCCCTCGTGAAATTACTTTCGAAGATGAAGAATTCGCTGCCTACCTCTAAAGTTCAAGAGGTTGCGACATTGTTTCAGACATTTAAGGTGAGTCTTTGTTGTGCAGGAAAATTGGGAAAAGATGTTCTTGTTAAAAGGCTGCGATCAATTGCTGGAGATGACATGCTTCTATCCTTAATTCGTGAAATTCGAGAATGTCAATGA

Coding sequence (CDS)

ATGGGTGACTGGGAAACGGATACATATGGCAGCACGCTAAGTTTCATCGCCTCGATTCTGCTGGTTCTTTCGAAGCGAAATTTCAGTGCCGGCTTTCGATCCCAGAGACTGGTAGAATCCATGGCCTCCTCTAAGCTTCTGATGCATAAGCAATTCAGGAAGAAGATGGTGGAATCGGTTGCGGTTCGGGCTCCTCCGAAGCCTTGTTCGAGAAACCCTATCGATTCCTCCTCTTGTCATCACTTATCCGATTCTTCGCAGTCTTTGATTCAAAACTACTCCAATTTCAAGGCCAGTGGTTTGCCTTCTCGTTTCATGTTCTATCAGGATAGGTTGTGGGTTGATTTCGATAATCGAGTGATTGGAATATTGCGAGCGGGTTTCTCCGACCGGAATCCGGCGATTGAACTGGAAATCGATGGATCGACGTATCTGTTTGATATGTATAGGATGTTACAGATTGATTTGGAAACTGGTAGACAGAGATCGGTTGCTTGGATCGATGAAAATGGGAAGTGTTTTTTCCCCAAAGTCTTTATCGGCGATGAGAGTGAGGGAAATACTCGGAGTTTGTCGAATCCGAAAATTGAAATTGAGATTAAGATTGATCGGAAGTCCGGGAAACGAAAGAGGGAAGCGATGGAGATGGAGGAGGAAGAAAATGAAGTGAATTCCAGCAATGAACATGTGGAAGTGAAGCCATTGAAAATACCGCGTGTTGTTGCGAATGATTCAGAAGCTCATGTATGGCCGAAGGCGAAGGCGCTGAGTGAAGGAGATAGTGCGTACACGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCCAGTTCTGCAATCACCTCAATTCATCAGTGCACCCGCACGGGGCCATTGGAAAAAGCTCGCCTCGATGTGTTTCAAAAGCAGAATGAGATTACAACGGCTGCCCGTGGTGTGTCCAATATGGTTTATGCATGGTATGGAGCTTCTGCAACAAGTTTGGCGGGAATTTTGGCTCATGGATTTGGGGAGCCTCTTCAGGTGCCTGCCGCCGATACTCATGGAATTGGAGTTTACTTATCTCCATTGGGCTTGCCTCATTTATGTTCTACGCTATCAGAGGTCGATGAGGATGGTATAAAACATATGGTTTTATGTCGAGTAATATTGGGTAATGTTGAAAAGGTTGAAGCAGGATCTCAGCAATCCCATCCGTCGAGTACAGAGTTTGACACTGGTGTGGATGATCCAACATGTCCTAAACGGTATATTGTCTGGTGTAGTAATATGAACAGACACATTTTACCAGAGTACATAGTGAGTTTCAGATCAAATTATCGTTTGCCAGGTAAGTTGGGGGAATCGACAGATACGAAGTATTCCCTCGTGAAATTACTTTCGAAGATGAAGAATTCGCTGCCTACCTCTAAAGTTCAAGAGGTTGCGACATTGTTTCAGACATTTAAGGTGAGTCTTTGTTGTGCAGGAAAATTGGGAAAAGATGTTCTTGTTAAAAGGCTGCGATCAATTGCTGGAGATGACATGCTTCTATCCTTAATTCGTGAAATTCGAGAATGTCAATGA

Protein sequence

MGDWETDTYGSTLSFIASILLVLSKRNFSAGFRSQRLVESMASSKLLMHKQFRKKMVESVAVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ
Homology
BLAST of CmoCh12G001850 vs. ExPASy Swiss-Prot
Match: Q8RY59 (Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RCD1 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-48
Identity = 118/380 (31.05%), Postives = 195/380 (51.32%), Query Frame = 0

Query: 85  SSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTY 144
           S ++L++ Y+ FK +G+  R M Y++  W D    VI  ++    +++ AIE ++ G ++
Sbjct: 72  SGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNELEEKSAAIEFKLCGHSF 131

Query: 145 LFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG--NTRSLSNPK------I 204
           + D   M ++D+ETG +  +AWID  GKCFFP+++  DE     + + + +PK      I
Sbjct: 132 ILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYCHHKCVEDPKQNAPHDI 191

Query: 205 EIEIKIDRKSGKRKREAMEMEEEENEVN----------SSNEHV-EVKPLKIPRVVANDS 264
           ++ ++ID   G+  R  +E   +E+  N          SSNEH  E       R +  ++
Sbjct: 192 KLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHYDEATEDSCSRKL--EA 251

Query: 265 EAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTR-----TGPLEKARL 324
               W +  A+    +  T        ++KK+      +  H         +  + +ARL
Sbjct: 252 AVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPVLDVGRFSSEIAEARL 311

Query: 325 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 384
            +FQKQ EIT   RG +N+ YAW  A    L+ ++  G G          +G+G++L+  
Sbjct: 312 ALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAFIRKSIYGVGIHLTAA 371

Query: 385 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 441
             P+  +   +VDE+G+++MVLCRVI+GN+E +     Q      E+D GVDD   PK Y
Sbjct: 372 DCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNY 431

BLAST of CmoCh12G001850 vs. ExPASy Swiss-Prot
Match: O82289 (Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3702 GN=SRO1 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 1.1e-47
Identity = 112/374 (29.95%), Postives = 190/374 (50.80%), Query Frame = 0

Query: 87  QSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLF 146
           +SL++ +S +K +G+P R MF+++  W+D  + ++  +R     +   IE    G  +L 
Sbjct: 73  KSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRATIEFNWCGRHFLL 132

Query: 147 DMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG-------NTRSLSNPKIEIE 206
           D   M ++DLETG +  +AWID  GKCFFP+ F   E +G       +       +I++ 
Sbjct: 133 DFLHMYRLDLETGVKTQLAWIDIAGKCFFPETFDTLERDGCHHIRGEDPEQHDQREIKLH 192

Query: 207 IKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEG 266
           I+ID  SG+  R  + +  +E+  N  +     +    P   A++          A+ + 
Sbjct: 193 IEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPNDEASEDSCS-RELDDAVEKW 252

Query: 267 DSAYTLVSNFFLPSMKKVDPSSA-------------ITSIHQCTRTGPLEKARLDVFQKQ 326
           D   T   +   P+ +++D  +              + S+     +  + KARL +FQKQ
Sbjct: 253 DKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQ 312

Query: 327 NEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPLGLPHLC 386
            +IT   RG +N+ YAW  A    L+ ++ HG G          +G+GV+ +    P+  
Sbjct: 313 ADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAA--NCPYFS 372

Query: 387 STLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSN 441
           +   ++D++G++HMVLCRVI+GN+E +   + Q      E+D GVDD   PK Y++W  N
Sbjct: 373 ARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMN 432

BLAST of CmoCh12G001850 vs. ExPASy Swiss-Prot
Match: O64592 (Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3702 GN=SRO3 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 9.3e-34
Identity = 94/313 (30.03%), Postives = 168/313 (53.67%), Query Frame = 0

Query: 214 MEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPS 273
           +E+E++E+  N  N  + + P      +++++          L E    + L+ N FL  
Sbjct: 5   VEIEDQESVTNLDNGEI-INP------ISDNAPNFSGDATILLREATFEHNLIKNCFLSG 64

Query: 274 MKKVDPSSAITSIHQ-CTRTGPLEKARLDVFQKQNE-ITTAARGVSNMVYAWYGASATSL 333
           M      + I ++ +  T+     KA+  VF+   E +     G +N+ Y WY  S   +
Sbjct: 65  MGSFATETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKNNGYANIRYGWYSGSKEEI 124

Query: 334 AGILAHGFG--EPLQVP-AADTHGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILG 393
             ++ +GF   E  +V     +HG+G++L         + + E DE+GIK+++LCRVILG
Sbjct: 125 DRVITYGFSNREIKKVENDVGSHGVGIHLVHHRYSLAAALVGEGDEEGIKNILLCRVILG 184

Query: 394 NVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGK 453
             E++  GS+QS+PSS +FD+GVD+   P++Y++W  NMN +ILP YIVSF+S + L G 
Sbjct: 185 KPEQIVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSYILPTYIVSFKS-HLLRGL 244

Query: 454 LGESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIA 513
           +G +     S   L+S +  SL  +++  + T +  F+       KL ++ LV+++R + 
Sbjct: 245 IGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFR-----KRKLRREQLVRKIREVV 304

Query: 514 GDDMLLSLIREIR 522
           GD++L  +++  R
Sbjct: 305 GDNLLFKILKNQR 304

BLAST of CmoCh12G001850 vs. ExPASy Swiss-Prot
Match: Q9ZUD9 (Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3702 GN=SRO2 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.1e-33
Identity = 86/271 (31.73%), Postives = 144/271 (53.14%), Query Frame = 0

Query: 256 LSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQ-CTRTGPLEKARLDVFQ-KQNEITTAA 315
           L EG+  + ++    L  M  V   + I +I +  +  G   +A+   F+   + +    
Sbjct: 48  LGEGNPEHDVIKTCLLSGMGVVSSDTTIVTISKNSSERGITTRAKFLAFRIFTDAVARKH 107

Query: 316 RGVSNMVYAWYGASATSLAGILAHGFGE---PLQVPAADTHGIGVYLSPLGLPHLCSTLS 375
            G +N+ Y WY  S   +  I+++GF             +HGIG++L P     L ++ +
Sbjct: 108 GGDANVKYGWYAGSRDEIQRIISYGFSNRDVGKFENDGGSHGIGIHLVPSKCSLLAASAT 167

Query: 376 EVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSNMNRH 435
           E DE+G+++++LCRVILG  E + +GS+QS+PSS EFD+GVDD   P+ Y++W  NMN  
Sbjct: 168 EQDEEGLRYLLLCRVILGKPEIIISGSKQSYPSSAEFDSGVDDLHNPRNYVIWSCNMNSC 227

Query: 436 ILPEYIVSFRS---NYRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFKV 495
           ILP YIVSFRS        G     +    S   L+S +  S+  S++  +   +  F+ 
Sbjct: 228 ILPSYIVSFRSPRLRVSRGGFASRPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFR- 287

Query: 496 SLCCAGKLGKDVLVKRLRSIAGDDMLLSLIR 519
                 K+ +D LV+++R +AGD++L  +I+
Sbjct: 288 ----KRKIRRDQLVRKMREVAGDNLLAEIIK 313

BLAST of CmoCh12G001850 vs. ExPASy Swiss-Prot
Match: Q9FJJ3 (Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3702 GN=SRO5 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.2e-27
Identity = 78/221 (35.29%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 302 VFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPLG 361
           VFQ+  ++     G + + Y W   S   L  I  +GF EPL+     + G G+YLSP  
Sbjct: 91  VFQEAVQMKHGGDGGAKVKYGWCSVSKHELKTIFEYGFSEPLRNDG--SFGRGLYLSPDN 150

Query: 362 LPHLC-STLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 421
            P  C    +   EDG++ ++LCRV+LG  E V  GS +S PSS EFD+GVDD    K+Y
Sbjct: 151 SPLDCLKDSASESEDGMRFLLLCRVLLGKSEIVPQGSTRSCPSSPEFDSGVDDLVSTKKY 210

Query: 422 IVWCSNMNRHILPEYIVSFRSNYRL---PGKLGESTDTKYSLVKLLSKMKNSLPTSKVQE 481
           IVW ++MN H+LPE++V  ++ + L   P +L         L+K LSK    LP S++  
Sbjct: 211 IVWSTHMNTHVLPEFLVCIKAPFNLTRSPKRLRSPWMAFPVLIKALSKF---LPPSQILV 270

Query: 482 VATLFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIR 519
           +   ++  +       ++ +  L++R+RSI GD +L+ +I+
Sbjct: 271 IQKHYKDQQ-----NRRITRSELIQRVRSITGDKLLVHIIK 301

BLAST of CmoCh12G001850 vs. ExPASy TrEMBL
Match: A0A6J1GJV8 (Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111454523 PE=4 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.4e-295
Identity = 519/524 (99.05%), Postives = 519/524 (99.05%), Query Frame = 0

Query: 1   MGDWETDTYGSTLSFIASILLVLSKRNFSAGFRSQRLVESMASSKLLMHKQFRKKMVESV 60
           MGDWETDTYGSTLSFIASILLVLSKRNFSAGFRSQRLVESMASSKLLMHKQFRKKMVESV
Sbjct: 1   MGDWETDTYGSTLSFIASILLVLSKRNFSAGFRSQRLVESMASSKLLMHKQFRKKMVESV 60

Query: 61  AVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRV 120
           AVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRV
Sbjct: 61  AVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRV 120

Query: 121 IGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFI 180
           IGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFI
Sbjct: 121 IGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFI 180

Query: 181 GDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRV 240
           GDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRV
Sbjct: 181 GDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRV 240

Query: 241 VANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTRTGPLEKARL 300
           VANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTRTGPLEKARL
Sbjct: 241 VANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTRTGPLEKARL 300

Query: 301 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 360
           DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL
Sbjct: 301 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 360

Query: 361 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 420
           GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY
Sbjct: 361 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 420

Query: 421 IVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVAT 480
           IVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVAT
Sbjct: 421 IVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVAT 480

Query: 481 LFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ 525
           LFQTFK     AGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ
Sbjct: 481 LFQTFK-----AGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ 519

BLAST of CmoCh12G001850 vs. ExPASy TrEMBL
Match: A0A6J1KNM2 (Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111496198 PE=4 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.0e-265
Identity = 467/484 (96.49%), Postives = 473/484 (97.73%), Query Frame = 0

Query: 41  MASSKLLMHKQFRKKMVESVAVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASG 100
           MASSKLLMHKQFRKKMVESVAV APP+PCSRNPI+SSSC+HLSDSSQSLIQNYSNFKASG
Sbjct: 1   MASSKLLMHKQFRKKMVESVAVPAPPRPCSRNPINSSSCNHLSDSSQSLIQNYSNFKASG 60

Query: 101 LPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR 160
           LPSRFMFYQDRLWVDFD +VIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR
Sbjct: 61  LPSRFMFYQDRLWVDFDTQVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR 120

Query: 161 QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEE 220
           QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIK D KSGKRKREAMEMEEEE
Sbjct: 121 QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKFDGKSGKRKREAMEMEEEE 180

Query: 221 NEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPS 280
           N VNSSNEHVEVKPLK+PRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPS+KKVDPS
Sbjct: 181 NYVNSSNEHVEVKPLKMPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSIKKVDPS 240

Query: 281 SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG 340
           SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG
Sbjct: 241 SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG 300

Query: 341 EPLQVPAADTHGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQS 400
           EPLQVPAADTHGIGVYLSPLGLPHLCSTLSE DEDGIKHMVLCRVILGNVEKVEAGSQQS
Sbjct: 301 EPLQVPAADTHGIGVYLSPLGLPHLCSTLSEADEDGIKHMVLCRVILGNVEKVEAGSQQS 360

Query: 401 HPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLV 460
           HPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLV
Sbjct: 361 HPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLV 420

Query: 461 KLLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIREI 520
           KLLSKMKNSLPTSKVQEVATLFQTFK     AGKLGKDVLVKRLRSIAGDDMLLSLIREI
Sbjct: 421 KLLSKMKNSLPTSKVQEVATLFQTFK-----AGKLGKDVLVKRLRSIAGDDMLLSLIREI 479

Query: 521 RECQ 525
           RECQ
Sbjct: 481 RECQ 479

BLAST of CmoCh12G001850 vs. ExPASy TrEMBL
Match: A0A6J1BQ52 (probable inactive poly [ADP-ribose] polymerase SRO3 OS=Momordica charantia OX=3673 GN=LOC111004739 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.1e-207
Identity = 381/502 (75.90%), Postives = 412/502 (82.07%), Query Frame = 0

Query: 24  SKRNFSAGFRSQRLVESMASSKLLMHKQFRKKMVESVAVRAPPKPCSRNPIDSSSCHHLS 83
           SKR  +  F  Q+LVESMAS      KQ  KKMVES+ V A P+PC RNP+        S
Sbjct: 24  SKRISAPAFDPQKLVESMAS------KQLGKKMVESIEVWASPRPCPRNPMS------FS 83

Query: 84  DSSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGST 143
           DS Q LIQNYSNFK SGLPSRFMFY+   W+DF  +V+  LRAGFS++ P +EL+I+GS 
Sbjct: 84  DSPQLLIQNYSNFKTSGLPSRFMFYRGSSWLDFPTQVLETLRAGFSEQRPVMELQIEGSK 143

Query: 144 YLFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKID 203
           YLFD+YRMLQID  TG QRSVAWIDENGKCFFPKVF+ +E         NPKIEIEI +D
Sbjct: 144 YLFDLYRMLQIDFNTGSQRSVAWIDENGKCFFPKVFVCEERANTYGDSWNPKIEIEITVD 203

Query: 204 RKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEA-HVWPKAKALSEGDSA 263
             SGKRKREA+EM  EE EVNS+NEHVE K  K+PRV+ANDSEA   WP  KAL+EGDSA
Sbjct: 204 GMSGKRKREAIEMNGEEYEVNSTNEHVEAKASKVPRVIANDSEALAAWPNVKALTEGDSA 263

Query: 264 YTLVSNFFLPSMKKVDPSSAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYA 323
           YTLVSNFFL SMKKVDP SAIT+IHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYA
Sbjct: 264 YTLVSNFFLSSMKKVDPGSAITAIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYA 323

Query: 324 WYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVL 383
           WYGASA +LAG LAHGFGEPLQ+PA DTHGIGVYLSP+GLPHLCS L   DEDG+KHM L
Sbjct: 324 WYGASAETLAGTLAHGFGEPLQLPANDTHGIGVYLSPVGLPHLCSKLLGADEDGVKHMAL 383

Query: 384 CRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSN 443
           CRVILGNVEKVEAGSQQSHPSS EFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSF+S 
Sbjct: 384 CRVILGNVEKVEAGSQQSHPSSPEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKST 443

Query: 444 YRLPGKLGESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVK 503
           Y L  KL ESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFK     AGKL KDVLVK
Sbjct: 444 YHLADKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFK-----AGKLAKDVLVK 503

Query: 504 RLRSIAGDDMLLSLIREIRECQ 525
           RLRSIAGD+MLLS+IREIR  Q
Sbjct: 504 RLRSIAGDEMLLSMIREIRGSQ 508

BLAST of CmoCh12G001850 vs. ExPASy TrEMBL
Match: A0A5A7VA62 (Poly [ADP-ribose] polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003920 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.3e-195
Identity = 355/483 (73.50%), Postives = 403/483 (83.44%), Query Frame = 0

Query: 43  SSKLLMHKQFRKKMVESVAVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLP 102
           +SKLLMH+Q RKKM+ S+ VR+P      NP +SSS    SDS  SL+ NYSNFK SGLP
Sbjct: 2   ASKLLMHEQLRKKMLGSIEVRSP----LGNPTNSSS----SDSPLSLLHNYSNFKKSGLP 61

Query: 103 SRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQR 162
           SRFMFY    WVDF  +V+ ILR  FSDRNPA+EL+IDGS YLFD+YRMLQIDL+TGRQR
Sbjct: 62  SRFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQR 121

Query: 163 SVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENE 222
           SV+WID+ G CFFPKVFIG+E+  ++    +PKIEIEI +D KSGKRKRE M    EENE
Sbjct: 122 SVSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETM----EENE 181

Query: 223 VNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSA 282
           V+SSNEH++VK  KIPRVV NDSE  VWPK K L+EGDS Y+LVSN  LPSMKKVD + +
Sbjct: 182 VSSSNEHMKVKASKIPRVVLNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFS 241

Query: 283 ITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEP 342
           I++IH+CTRTGPLEKARLDVF KQNEIT AARGVSNMVYAWYGASA +LAGILAHGFGEP
Sbjct: 242 ISAIHRCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEP 301

Query: 343 LQVPAAD-THGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSH 402
           +Q+PA+D THGIGVYLSP GLPHL S LSE D +G+KHM+LCRVILGN+EK+EAGS+QSH
Sbjct: 302 VQIPASDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSH 361

Query: 403 PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVK 462
           PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSF+S+  LPGKL EST+TKY L K
Sbjct: 362 PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAK 421

Query: 463 LLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIREIR 522
           LLSKM+NSLP SKVQEVATLFQ FKV     G+L KDVLVKRLRSIAGD+MLLS+ RE R
Sbjct: 422 LLSKMRNSLPPSKVQEVATLFQKFKV-----GQLAKDVLVKRLRSIAGDEMLLSIFRESR 467

Query: 523 ECQ 525
            CQ
Sbjct: 482 GCQ 467

BLAST of CmoCh12G001850 vs. ExPASy TrEMBL
Match: A0A1S3BCD4 (Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103488157 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.3e-195
Identity = 355/483 (73.50%), Postives = 403/483 (83.44%), Query Frame = 0

Query: 43  SSKLLMHKQFRKKMVESVAVRAPPKPCSRNPIDSSSCHHLSDSSQSLIQNYSNFKASGLP 102
           +SKLLMH+Q RKKM+ S+ VR+P      NP +SSS    SDS  SL+ NYSNFK SGLP
Sbjct: 2   ASKLLMHEQLRKKMLGSIEVRSP----LGNPTNSSS----SDSPLSLLHNYSNFKKSGLP 61

Query: 103 SRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGRQR 162
           SRFMFY    WVDF  +V+ ILR  FSDRNPA+EL+IDGS YLFD+YRMLQIDL+TGRQR
Sbjct: 62  SRFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQR 121

Query: 163 SVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEENE 222
           SV+WID+ G CFFPKVFIG+E+  ++    +PKIEIEI +D KSGKRKRE M    EENE
Sbjct: 122 SVSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETM----EENE 181

Query: 223 VNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSA 282
           V+SSNEH++VK  KIPRVV NDSE  VWPK K L+EGDS Y+LVSN  LPSMKKVD + +
Sbjct: 182 VSSSNEHMKVKASKIPRVVMNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFS 241

Query: 283 ITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEP 342
           I++IH+CTRTGPLEKARLDVF KQNEIT AARGVSNMVYAWYGASA +LAGILAHGFGEP
Sbjct: 242 ISAIHRCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEP 301

Query: 343 LQVPAAD-THGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSH 402
           +Q+PA+D THGIGVYLSP GLPHL S LSE D +G+KHM+LCRVILGN+EK+EAGS+QSH
Sbjct: 302 VQIPASDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSH 361

Query: 403 PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSLVK 462
           PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSF+S+  LPGKL EST+TKY L K
Sbjct: 362 PSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAK 421

Query: 463 LLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIAGDDMLLSLIREIR 522
           LLSKM+NSLP SKVQEVATLFQ FKV     G+L KDVLVKRLRSIAGD+MLLS+ RE R
Sbjct: 422 LLSKMRNSLPPSKVQEVATLFQKFKV-----GQLAKDVLVKRLRSIAGDEMLLSIFRESR 467

Query: 523 ECQ 525
            CQ
Sbjct: 482 GCQ 467

BLAST of CmoCh12G001850 vs. TAIR 10
Match: AT1G32230.1 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 118/380 (31.05%), Postives = 195/380 (51.32%), Query Frame = 0

Query: 85  SSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTY 144
           S ++L++ Y+ FK +G+  R M Y++  W D    VI  ++    +++ AIE ++ G ++
Sbjct: 72  SGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNELEEKSAAIEFKLCGHSF 131

Query: 145 LFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG--NTRSLSNPK------I 204
           + D   M ++D+ETG +  +AWID  GKCFFP+++  DE     + + + +PK      I
Sbjct: 132 ILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYCHHKCVEDPKQNAPHDI 191

Query: 205 EIEIKIDRKSGKRKREAMEMEEEENEVN----------SSNEHV-EVKPLKIPRVVANDS 264
           ++ ++ID   G+  R  +E   +E+  N          SSNEH  E       R +  ++
Sbjct: 192 KLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHYDEATEDSCSRKL--EA 251

Query: 265 EAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTR-----TGPLEKARL 324
               W +  A+    +  T        ++KK+      +  H         +  + +ARL
Sbjct: 252 AVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPVLDVGRFSSEIAEARL 311

Query: 325 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 384
            +FQKQ EIT   RG +N+ YAW  A    L+ ++  G G          +G+G++L+  
Sbjct: 312 ALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAFIRKSIYGVGIHLTAA 371

Query: 385 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 441
             P+  +   +VDE+G+++MVLCRVI+GN+E +     Q      E+D GVDD   PK Y
Sbjct: 372 DCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNY 431

BLAST of CmoCh12G001850 vs. TAIR 10
Match: AT1G32230.2 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 118/380 (31.05%), Postives = 195/380 (51.32%), Query Frame = 0

Query: 85  SSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTY 144
           S ++L++ Y+ FK +G+  R M Y++  W D    VI  ++    +++ AIE ++ G ++
Sbjct: 72  SGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNELEEKSAAIEFKLCGHSF 131

Query: 145 LFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG--NTRSLSNPK------I 204
           + D   M ++D+ETG +  +AWID  GKCFFP+++  DE     + + + +PK      I
Sbjct: 132 ILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYCHHKCVEDPKQNAPHDI 191

Query: 205 EIEIKIDRKSGKRKREAMEMEEEENEVN----------SSNEHV-EVKPLKIPRVVANDS 264
           ++ ++ID   G+  R  +E   +E+  N          SSNEH  E       R +  ++
Sbjct: 192 KLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHYDEATEDSCSRKL--EA 251

Query: 265 EAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTR-----TGPLEKARL 324
               W +  A+    +  T        ++KK+      +  H         +  + +ARL
Sbjct: 252 AVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPVLDVGRFSSEIAEARL 311

Query: 325 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 384
            +FQKQ EIT   RG +N+ YAW  A    L+ ++  G G          +G+G++L+  
Sbjct: 312 ALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAFIRKSIYGVGIHLTAA 371

Query: 385 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 441
             P+  +   +VDE+G+++MVLCRVI+GN+E +     Q      E+D GVDD   PK Y
Sbjct: 372 DCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNY 431

BLAST of CmoCh12G001850 vs. TAIR 10
Match: AT1G32230.3 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 118/380 (31.05%), Postives = 195/380 (51.32%), Query Frame = 0

Query: 85  SSQSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTY 144
           S ++L++ Y+ FK +G+  R M Y++  W D    VI  ++    +++ AIE ++ G ++
Sbjct: 72  SGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNELEEKSAAIEFKLCGHSF 131

Query: 145 LFDMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG--NTRSLSNPK------I 204
           + D   M ++D+ETG +  +AWID  GKCFFP+++  DE     + + + +PK      I
Sbjct: 132 ILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYCHHKCVEDPKQNAPHDI 191

Query: 205 EIEIKIDRKSGKRKREAMEMEEEENEVN----------SSNEHV-EVKPLKIPRVVANDS 264
           ++ ++ID   G+  R  +E   +E+  N          SSNEH  E       R +  ++
Sbjct: 192 KLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHYDEATEDSCSRKL--EA 251

Query: 265 EAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPSSAITSIHQCTR-----TGPLEKARL 324
               W +  A+    +  T        ++KK+      +  H         +  + +ARL
Sbjct: 252 AVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPVLDVGRFSSEIAEARL 311

Query: 325 DVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPL 384
            +FQKQ EIT   RG +N+ YAW  A    L+ ++  G G          +G+G++L+  
Sbjct: 312 ALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAFIRKSIYGVGIHLTAA 371

Query: 385 GLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRY 441
             P+  +   +VDE+G+++MVLCRVI+GN+E +     Q      E+D GVDD   PK Y
Sbjct: 372 DCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNY 431

BLAST of CmoCh12G001850 vs. TAIR 10
Match: AT2G35510.1 (similar to RCD one 1 )

HSP 1 Score: 192.6 bits (488), Expect = 8.1e-49
Identity = 112/374 (29.95%), Postives = 190/374 (50.80%), Query Frame = 0

Query: 87  QSLIQNYSNFKASGLPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLF 146
           +SL++ +S +K +G+P R MF+++  W+D  + ++  +R     +   IE    G  +L 
Sbjct: 73  KSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRATIEFNWCGRHFLL 132

Query: 147 DMYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIGDESEG-------NTRSLSNPKIEIE 206
           D   M ++DLETG +  +AWID  GKCFFP+ F   E +G       +       +I++ 
Sbjct: 133 DFLHMYRLDLETGVKTQLAWIDIAGKCFFPETFDTLERDGCHHIRGEDPEQHDQREIKLH 192

Query: 207 IKIDRKSGKRKREAMEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEG 266
           I+ID  SG+  R  + +  +E+  N  +     +    P   A++          A+ + 
Sbjct: 193 IEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPNDEASEDSCS-RELDDAVEKW 252

Query: 267 DSAYTLVSNFFLPSMKKVDPSSA-------------ITSIHQCTRTGPLEKARLDVFQKQ 326
           D   T   +   P+ +++D  +              + S+     +  + KARL +FQKQ
Sbjct: 253 DKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQ 312

Query: 327 NEITTAARGVSNMVYAWYGASATSLAGILAHGFGEPLQVPAADTHGIGVYLSPLGLPHLC 386
            +IT   RG +N+ YAW  A    L+ ++ HG G          +G+GV+ +    P+  
Sbjct: 313 ADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAA--NCPYFS 372

Query: 387 STLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSN 441
           +   ++D++G++HMVLCRVI+GN+E +   + Q      E+D GVDD   PK Y++W  N
Sbjct: 373 ARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMN 432

BLAST of CmoCh12G001850 vs. TAIR 10
Match: AT1G70440.1 (similar to RCD one 3 )

HSP 1 Score: 146.4 bits (368), Expect = 6.6e-35
Identity = 94/313 (30.03%), Postives = 168/313 (53.67%), Query Frame = 0

Query: 214 MEMEEEENEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPS 273
           +E+E++E+  N  N  + + P      +++++          L E    + L+ N FL  
Sbjct: 5   VEIEDQESVTNLDNGEI-INP------ISDNAPNFSGDATILLREATFEHNLIKNCFLSG 64

Query: 274 MKKVDPSSAITSIHQ-CTRTGPLEKARLDVFQKQNE-ITTAARGVSNMVYAWYGASATSL 333
           M      + I ++ +  T+     KA+  VF+   E +     G +N+ Y WY  S   +
Sbjct: 65  MGSFATETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKNNGYANIRYGWYSGSKEEI 124

Query: 334 AGILAHGFG--EPLQVP-AADTHGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILG 393
             ++ +GF   E  +V     +HG+G++L         + + E DE+GIK+++LCRVILG
Sbjct: 125 DRVITYGFSNREIKKVENDVGSHGVGIHLVHHRYSLAAALVGEGDEEGIKNILLCRVILG 184

Query: 394 NVEKVEAGSQQSHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGK 453
             E++  GS+QS+PSS +FD+GVD+   P++Y++W  NMN +ILP YIVSF+S + L G 
Sbjct: 185 KPEQIVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSYILPTYIVSFKS-HLLRGL 244

Query: 454 LGESTDTKYSLVKLLSKMKNSLPTSKVQEVATLFQTFKVSLCCAGKLGKDVLVKRLRSIA 513
           +G +     S   L+S +  SL  +++  + T +  F+       KL ++ LV+++R + 
Sbjct: 245 IGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFR-----KRKLRREQLVRKIREVV 304

Query: 514 GDDMLLSLIREIR 522
           GD++L  +++  R
Sbjct: 305 GDNLLFKILKNQR 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RY591.7e-4831.05Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RC... [more]
O822891.1e-4729.95Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3... [more]
O645929.3e-3430.03Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3... [more]
Q9ZUD92.1e-3331.73Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3... [more]
Q9FJJ32.2e-2735.29Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GJV82.4e-29599.05Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111454523 PE=4 ... [more]
A0A6J1KNM22.0e-26596.49Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111496198 PE=4 SV... [more]
A0A6J1BQ521.1e-20775.90probable inactive poly [ADP-ribose] polymerase SRO3 OS=Momordica charantia OX=36... [more]
A0A5A7VA621.3e-19573.50Poly [ADP-ribose] polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BCD41.3e-19573.50Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103488157 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G32230.11.2e-4931.05WWE protein-protein interaction domain protein family [more]
AT1G32230.21.2e-4931.05WWE protein-protein interaction domain protein family [more]
AT1G32230.31.2e-4931.05WWE protein-protein interaction domain protein family [more]
AT2G35510.18.1e-4929.95similar to RCD one 1 [more]
AT1G70440.16.6e-3530.03similar to RCD one 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 202..229
NoneNo IPR availableGENE3D3.90.228.10coord: 252..441
e-value: 3.6E-31
score: 110.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..227
NoneNo IPR availablePANTHERPTHR32263:SF19OS03G0230300 PROTEINcoord: 87..520
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 256..442
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPFAMPF00644PARPcoord: 301..394
e-value: 1.6E-6
score: 27.8
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPROSITEPS51059PARP_CATALYTICcoord: 239..460
score: 29.86286
IPR022003RST domainPFAMPF12174RSTcoord: 460..521
e-value: 2.0E-7
score: 30.8
IPR022003RST domainPROSITEPS51879RSTcoord: 450..524
score: 15.174485
IPR044964Inactive poly [ADP-ribose] polymerase RCD1/SRO1-5PANTHERPTHR32263INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATEDcoord: 87..520
IPR004170WWE domainPROSITEPS50918WWEcoord: 91..166
score: 10.808201
IPR037197WWE domain superfamilySUPERFAMILY117839WWE domaincoord: 110..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G001850.1CmoCh12G001850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity