CmoCh12G001650 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh12G001650 vs. ExPASy Swiss-Prot
Match: Q8GY23 (E3 ubiquitin-protein ligase UPL1 OS=Arabidopsis thaliana OX=3702 GN=UPL1 PE=1 SV=3)

HSP 1 Score: 2213.3 bits (5734), Expect = 0.0e+00
Identity = 1525/3839 (39.72%), Postives = 2175/3839 (56.66%), Query Frame = 0

Query: 30   SEPPPKIKAFIDKVIQCPLHDIAIHLSGFRWEYNKGNYHHWRPLFLHFDTYFKTYLSCRK 89
            SE P KIK+FI+ V   PL  I   L+ FRWE++KG++HHW  LF +FDT+F+ ++  RK
Sbjct: 9    SEVPSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDTFFEKHVQVRK 68

Query: 90   DL-LSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLE-YFNLLLASTDPEILI 149
            DL + +   E D PFPK A+LQ+LRV+++VLENC NK      E + +LLLASTD +++ 
Sbjct: 69   DLHIEENFEESDPPFPKDAVLQVLRVIRVVLENCTNKHFYSSYEQHLSLLLASTDADVVE 128

Query: 150  AALETLSALVKINPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCVVADEITR 209
            A L+TL+A +K    K   R       S+NS L SLAQGWG KEEGLGL SC        
Sbjct: 129  ACLQTLAAFLKRQIGKYSIRD-----ASLNSKLFSLAQGWGGKEEGLGLTSCA------- 188

Query: 210  EEGRCLFPHDVENDTNNAQYRIGSSLYFELHGCGAKSSDESSSSSSSSNSRVIHIPDLHL 269
                       EN  +    ++G +L+FE +      SDE S S      +VIH+PD+ +
Sbjct: 189  ----------AENSCDQVSLQLGRTLHFEFY-----PSDE-SPSELPGGLQVIHVPDVSI 248

Query: 270  KKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLAFIVLVQSG 329
              E DL +L + +  ++VPP LRF+LLTR+R+ARAF S   R+ ++ I L AF+VLVQ+ 
Sbjct: 249  CAESDLELLNKLVIDHNVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQAS 308

Query: 330  DSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSHERRILSGS 389
               + +V+FF  EPE+ NEL+ +V  E+TVP  IR L + +L A  S   + +  +L+  
Sbjct: 309  GDTENVVSFFNGEPEFVNELVTLVSYEDTVPEKIRILCLLSLVA-LSQDRTRQPTVLTAV 368

Query: 390  SISFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLHMVSSSASGNNIRSSG 449
            +   +GG+R +L  ++QKAI S+       SLAF EALL    + +VSSS+  + +R +G
Sbjct: 369  T---SGGHRGLLSGLMQKAIDSVVCITSKWSLAFAEALLSLVTV-LVSSSSGCSAMREAG 428

Query: 450  MVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKELGGVEILVERLQTEVHR 509
            ++PT +PL++D+DP HLHLV  AV  L+  MD+S+ A +LF++LGG++  + RL+ EV R
Sbjct: 429  LIPTLVPLIKDTDPQHLHLVSAAVHILEAFMDYSNPAAALFRDLGGLDDTIFRLKLEVSR 488

Query: 510  VIGLSGNNIDSMIIGESSRCNDDQL--------YNQKRMIKVSLKALGVATYVP------ 569
                  +  +     +S+  + +QL        Y+++ ++K  L+A+ + TY P      
Sbjct: 489  T---EDDVKEKNCSSDSNGPDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLY 548

Query: 570  TSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLLHELGLPDAFLASV 629
             S  + LP+ L +IF     FGG ++  A T+MS++IHKDPTC+  L   GL   FL ++
Sbjct: 549  GSEESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSTFLDAI 608

Query: 630  AAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKEKYVLVM-NEAIVP 689
            +  ++ S +AITC+P  + A+CLN  GL+AVK+  ALR  + IFT   Y+  +  +    
Sbjct: 609  SDEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFTSPSYLRALTGDTPGS 668

Query: 690  LANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGNTA-----METDS 749
            L++ ++EL RH SSLR+ GVD+ +E++  +  +G      +S   +  T+     ME D 
Sbjct: 669  LSSGLDELLRHQSSLRTYGVDMFIEILNSMLIIGSGMEATTSKSADVPTSAAPVPMEIDV 728

Query: 750  DDKDNNSIVTEEV--------INTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEA 809
            D+K  +  V++E          N E  +  C+ ++  L    ++N+E C +FVE  GI+A
Sbjct: 729  DEK--SLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDA 788

Query: 810  LLKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVS 869
            +L+L   P +  S   ++    ++ FK F+  HSA LAR  CS LR+HLKK       + 
Sbjct: 789  VLQLFSLPLMPLST--SLGQSFSVAFKNFSPQHSAGLARIVCSYLREHLKKTKILLVSIE 848

Query: 870  GSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHR 929
            G+ LL   +    KI  SL  +E +L L++    +  A++++E      +VL+++G  ++
Sbjct: 849  GTQLLKLESAIQTKILRSLSCLEGMLSLSNFL-LKGSASVISELSAADADVLKELGITYK 908

Query: 930  QILWQIALLEDVK-PELEDESTGSITELQ-NSEVHTNEIEEQRFNSFR-QFLDPLLRRRT 989
            Q +WQ+AL  D K  E +    GS   +  +S     E +E   N+   ++ +P+  R +
Sbjct: 909  QTIWQMALCNDTKEDEKKSVDRGSDNSVSASSSTAERESDEDSSNALAVRYTNPVSIRSS 968

Query: 990  SG---WSVESQFFDLINLYRDL-GRAPSSLQRM--GPNGSPLLQFGANNQRRSESSDTIG 1049
            S    W  + +F  ++     + GR   ++ RM  G     L  F  +    SE    + 
Sbjct: 969  SSQSIWGGDREFLSIVRSGEGIHGRTRHAIARMRGGRTRRHLESFNFD----SEIPADLP 1028

Query: 1050 ASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVA 1109
             ++     K ++      +++  L+         L K     +RRR D  ++SS+SK + 
Sbjct: 1029 VTSSSHELKKKSTEVLIAEILNKLNCTLRFFFTALVKGFTSANRRRIDGASLSSASKTLG 1088

Query: 1110 STLSSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLD-RPDSCNTVLLNCL 1169
            + L+ + L+ +NF G+  A+G E S+S KCRY GKV+D +  +  D R   C T ++N  
Sbjct: 1089 TALAKVFLEALNFDGYGAAAGHEKSLSVKCRYLGKVVDDITFLSFDTRRRVCFTAMVNSF 1148

Query: 1170 YGHGVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHS-WIQGPLASYG 1229
            Y HG    +LTTFEATSQLL+T+   P S   +   N K  E+    HS W+   L +Y 
Sbjct: 1149 YVHGTFKELLTTFEATSQLLWTV---PFSIPASSTENEKPGERNIWSHSKWLVDTLQNYC 1208

Query: 1230 RLMDHLVTSPFILSSFTK-HLLAQP----LTSGDVPFPRDAETFVKVLQSMVLKAVLPVW 1289
            R +D+ V S ++LS  ++  LL QP    L+ G  P PR+ ETFV+ LQS VL  +LP+W
Sbjct: 1209 RALDYFVNSTYLLSPTSQTQLLVQPASVGLSIGLFPVPREPETFVRNLQSQVLDVILPIW 1268

Query: 1290 THPQFIDCSCEFITTVISIIRHIYSGV-----EVKSVSSNSSARIAGPPPNETAISTIVE 1349
             HP F DC+  F+ +V S++ HIYSGV         V+   + R      +E+ +  IVE
Sbjct: 1269 NHPMFPDCNPNFVASVTSLVTHIYSGVVDARENRSGVTRGINQRALPLQLDESIVGMIVE 1328

Query: 1350 MGFSRGRAEEALRQVGSNSVELAMEWLFSHPEE-VQEDDELARALALSLGN-SELEMKEP 1409
            MGFSR RAE ALR+VG+NSVE+AM+WLF++PE+ VQEDDELA+ALALSLGN SE    E 
Sbjct: 1329 MGFSRSRAEIALRRVGTNSVEMAMDWLFTNPEQPVQEDDELAQALALSLGNSSETPKLED 1388

Query: 1410 VSSEVS-KQIEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVM 1469
                V   Q E   + P  +E+++  V+L ++ +++AFP+ DL V +C++N G++R  ++
Sbjct: 1389 TEKPVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSMAFPLMDLFVTLCNRNKGEDRPKIV 1448

Query: 1470 SFLIESVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWD 1529
            S+LI+ +K   ++  S ++  L+ + H++AL+L++D   RE A Q+G+VTV+  +L+ ++
Sbjct: 1449 SYLIQQLK-LVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVTVAIGILTDFN 1508

Query: 1530 SGLSFGVKVEVPKWVTAAFLAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTID--EDKQN 1589
                   ++  PK ++A  L +  +LQ + K + E  +     N GG  +  D  +D   
Sbjct: 1509 LKSESETEILAPKCISALLLVLSMMLQAQTKLSSEYVE----GNQGGSLVPSDSPQDSTA 1568

Query: 1590 KLQSAL-----------------GLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVLQ 1649
             L+ AL                 G S  Y+ +    + + IAC  IK+ +P   M AVLQ
Sbjct: 1569 ALKDALSSDVAKGESNQALELIFGKSTGYLTMEEGHKALLIACGLIKQHVPAMIMQAVLQ 1628

Query: 1650 LCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDPQTLQQAMES 1709
            LC+ LT+SH++A+ FLE GGL+SL +LP    FPG+D++AS I+RH++EDPQTLQ AME+
Sbjct: 1629 LCARLTKSHALAIQFLENGGLSSLFNLPKKCCFPGYDTVASVIVRHLVEDPQTLQIAMET 1688

Query: 1710 EIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIEMIGERPYVVLL 1769
            EIR TL  +  RH  GRV PR FL  +  VISRDPV+FM+A  S CQ+E  G R +V+L 
Sbjct: 1689 EIRQTL--SGKRH-IGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGRDFVILS 1748

Query: 1770 KDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD--SRSSRVHK 1829
            K++EK K    +                +  PLG +         +K HD   + S+ H+
Sbjct: 1749 KEKEKPKVSGSE------------HGFSLNEPLGIS--------ENKLHDVSGKCSKSHR 1808

Query: 1830 KSSQNFINVIELLLESVYTFIPPMK--DDVTVELTSGAQASSDMDIDVAAVKGKGKAIAS 1889
            +   NFI VI+ L++ V +F P +K  +D    L S       M++D    K KGK+   
Sbjct: 1809 RVPANFIQVIDQLIDLVLSF-PRVKRQEDGETNLIS-------MEVDEPTTKVKGKSKVG 1868

Query: 1890 LSEDDANSQ----------EASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSR 1949
              E  ++S+          E S  LA+V FILKLL++I+LMY+    V++RRDTE+   R
Sbjct: 1869 EPEKASSSRVGEPEKAEIPEKSEELARVTFILKLLSDIVLMYSHGTSVILRRDTEISQLR 1928

Query: 1950 PVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKV-DGDWKHKLATRGSQFLVASCVRS 2009
              +  + + G  GG+I+H++H  +P+S     EK V   +WK KL+ + S FLV  C RS
Sbjct: 1929 GSNLPDDSPG-NGGLIYHVIHRLLPIS----LEKFVGPEEWKEKLSEKASWFLVVLCSRS 1988

Query: 2010 SEARRRIFVEVGSILNQFIDSCNGSRP----PSSDLQAFVDLLNDMLAARTPT------G 2069
            +E R+RI  E+  +L+ F      S      P   + AF +L+  +L   + +      G
Sbjct: 1989 NEGRKRIINELSRVLSVFASLGRSSSKSVLLPDKRVLAFANLVYSILTKNSSSSSSNFPG 2048

Query: 2070 SYITTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTK-----EHVHF 2129
               + + + + +D G ++  T IL V+DLDHPD+PK+VT ++K+LE +T+     E +  
Sbjct: 2049 CGCSPDVAKSMMDGGTIQCLTSILHVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKS 2108

Query: 2130 GDSNAGKGDSSSKTPDHH----QPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQI 2189
               N  K   S +  D H    +   + + +   +++ V+ +        Q+   +A + 
Sbjct: 2109 EVPNEKKNRDSDERHDSHGNSTETEADELNQNNSSLQQVTDAAGNGQEQAQVSSQSAGE- 2168

Query: 2190 YGGSEAVTDDMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPEN 2249
             G S+  T  M  D  + G      E   M    EE  G          QIE+  HV   
Sbjct: 2169 RGSSQ--TQAMPQDMRIEGDETILPEPIQMDFMREEIEG---------DQIEMSFHVENR 2228

Query: 2250 LDEDDDEEMSGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEID--DD 2309
             D+D D++M    GDE ++DE +DED       DL +D    +    TD +D E     D
Sbjct: 2229 ADDDVDDDM----GDEGEDDEGDDEDA------DLVEDGAGVMSLAGTDVEDPEDTGLGD 2288

Query: 2310 EFDEVLEEDDEDDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQN---ETLHVMPVE- 2369
            E+++ + ++D+DD  +   + +R  E ++G+   DH ++ GR    N   + +   P E 
Sbjct: 2289 EYNDDMVDEDDDDFHENRVIEVRWREALDGL---DHFQILGRSGGGNGFIDDITAEPFEG 2348

Query: 2370 -----IFGSRR----QGRTTSIYNLLGRTGDTVAPSRHPLLGGPG--------------- 2429
                 +F  RR    + R  +  + L R+G  V   +HPL   P                
Sbjct: 2349 VNVDDLFALRRPLGFERRRQTGRSSLDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSI 2408

Query: 2430 ---------------LHATPFRPSENNRDMVISERSLENNSSG--LDTVFRSLRSGRHGH 2489
                           +  TP  P +       S R     +     D     + S R G 
Sbjct: 2409 SRHSEAGSYDVAQFYMFDTPVLPFDQVPVDPFSARLAGGGAPPPLTDYSVVGMDSSRRGV 2468

Query: 2490 RLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQT 2549
              + W +       S +  I Q +EE  +S LR   P        N   +      +IQ 
Sbjct: 2469 GDSRWTDIGHPQPSSLSASIAQLIEEHFISNLRASAP-------VNTVVERETNTTEIQE 2528

Query: 2550 S-----EPSGRSETIVENSGQHG-------------NDNLPPPVAINSSDGTNSRPAA-- 2609
                   PS  SET++ +  + G             N N PP V   S     +  A+  
Sbjct: 2529 QLHPDVPPSVGSETVLGDGNEGGQQSEERELLNNNENVNNPPDVMAESFAQGQANLASPV 2588

Query: 2610 ----SESLQGTQVIQ----QSQPVE---MQFEHNDAAVRDV---EAVSQESGGSGATFGE 2669
                 ESLQ  +V+Q     S P E   M+    D A  D    EAV   S   G     
Sbjct: 2589 SQDTGESLQQLEVMQPLPLNSTPNEIDRMEVGEGDGAPIDQVDHEAVHLISTAQGQPDTS 2648

Query: 2670 SLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMRRSNVSFTNSASLSGRDTSLHI 2729
            S++++ V   +    D   + Q S D     D                + L G +  L  
Sbjct: 2649 SIQNVSVTAIAPPVDDPDSNFQPSVDVDMSSDGAEGNQS------VQPSPLDGDNNELSS 2708

Query: 2730 VTEVSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDALPEELRAEVLSTQQGQVVQPP 2789
            +       + E  EEG +  +       + AIDP FL+ALPE+LRAEVL++QQ Q VQPP
Sbjct: 2709 MEATENVRNDEQVEEGSLDGRAPE----VNAIDPTFLEALPEDLRAEVLASQQAQSVQPP 2768

Query: 2790 SNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQELEGQPVEMDTVSIIATFPSD 2849
            + EP    DIDPEFLAALPPDI+ EVLAQQ+AQR  + Q+ +GQ V+MD  SIIAT P+D
Sbjct: 2769 TYEPPPVDDIDPEFLAALPPDIQTEVLAQQRAQR--MVQQSQGQAVDMDNASIIATLPAD 2828

Query: 2850 LREEVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR-SLFGMHPR--NRRGEITRRV 2909
            LREEVLLTSS+A+LA L   L+AEA MLR+R    Y  R S+FG   R  NRR  +    
Sbjct: 2829 LREEVLLTSSEAVLAALPSPLLAEAQMLRDRAMSHYQARSSVFGSSHRLNNRRNGL--GY 2888

Query: 2910 EGISGLDRTGGSISSRRSL-----GSRLIEAEGAPLVDTDALHAMIRLLRVVQPLYKGQL 2969
              ++G+DR  G    +R++     G ++ E EG PLV+ DAL ++IRLLR+ QPL KG L
Sbjct: 2889 NRLTGMDRGVGVTIGQRAVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLL 2948

Query: 2970 QRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELS----YRLYACQRNVIYSR 3029
            QRLLLNLCAH+ TR +LV++L+D++     +P  +++ +EL+     RLY CQ NV+Y R
Sbjct: 2949 QRLLLNLCAHSFTRANLVQLLLDMI-----RPEMETSPSELAITNPQRLYGCQSNVVYGR 3008

Query: 3030 PSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAV 3089
                +G PPLV RRVLE LTYLA NH  VA +L    +F  +L    +  +   K     
Sbjct: 3009 SQLLNGLPPLVFRRVLEVLTYLATNHSAVADMLF---YFDSSLLSQLSSRKGKEKVTHVT 3068

Query: 3090 DRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAESKSHLSDQP 3149
            D  +++        + + + L LLN+P  L+S +HL  ++ LL+V++  A S+       
Sbjct: 3069 DSRDLE--------IPLVVFLKLLNRPQLLQSTSHLGLVMGLLQVVVYTAASR------- 3128

Query: 3150 TQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGEFDIHSILAN 3209
             +        PE  L +  V G+ +S  +     S+L     ++ +      +I++I   
Sbjct: 3129 IEGWSPSSGVPE-KLENKPV-GEEASSETRKDAESELVGEADLSVARRKNCAEIYNIFLQ 3188

Query: 3210 LPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAM 3269
            LP+++L  LC LL  EGLSD  Y+L GEV+KKL  +   H + F  ELSE    L+ S +
Sbjct: 3189 LPQSDLCNLCILLGYEGLSDKIYSLAGEVLKKLAAVDVAHRKFFTKELSELASSLSSSTV 3248

Query: 3270 DELHMFGEAVK-ALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKEHASALTLV 3329
             EL       K + S  S  GA+ILRVLQ LSSL + + E    +    E+E  +   ++
Sbjct: 3249 RELATLSSKQKMSRSTGSMAGASILRVLQVLSSLTSPIDESNVGTERETEQEEQN---IM 3308

Query: 3330 CDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTP--PLPAGSQNILP 3389
              +N ALEPLW ELS CI   E   D +     S   P     G++P   L  G+Q +LP
Sbjct: 3309 QRLNVALEPLWHELSQCISMTELQLDHT--AAASNINPGDHVLGISPTSSLSPGTQRLLP 3368

Query: 3390 YIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRF 3449
             IE+FFV+CEK+    P   Q+    T  EV+++ A     +T+V +QK  +  + F +F
Sbjct: 3369 LIEAFFVLCEKIQ--TPSMLQQDTNVTAGEVKESSAHGSSSKTSVDSQKKTDGSVTFSKF 3428

Query: 3450 SEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHH--SPLRI 3509
            +EKHR+LLN+FIRQNP LLEKS S MLK PR IDFDNK+++FRSRI+HQHD H   PLRI
Sbjct: 3429 AEKHRRLLNSFIRQNPSLLEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRI 3488

Query: 3510 SVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGAL 3569
            SVRRAY+LEDSYNQLRMRS QDLKGRL V FQGEEGIDAGGL+REWYQ+LSRVIFDKGAL
Sbjct: 3489 SVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGAL 3548

Query: 3570 LFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVK 3629
            LFTTVGND+TFQPNPNS YQTEHLSYFKFVGR+V KAL+DGQLLDV+FTRSFYKHILGVK
Sbjct: 3549 LFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVK 3608

Query: 3630 VTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGR 3664
            VTYHDIEA+DPDYYKNLKW+LEND+SD+L LTFS+DADEEK ILYEKTEVTDYEL PGGR
Sbjct: 3609 VTYHDIEAVDPDYYKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGR 3668

BLAST of CmoCh12G001650 vs. ExPASy Swiss-Prot
Match: Q8H0T4 (E3 ubiquitin-protein ligase UPL2 OS=Arabidopsis thaliana OX=3702 GN=UPL2 PE=1 SV=3)

HSP 1 Score: 2206.0 bits (5715), Expect = 0.0e+00
Identity = 1497/3817 (39.22%), Postives = 2170/3817 (56.85%), Query Frame = 0

Query: 30   SEPPPKIKAFIDKVIQCPLHDIAIHLSGFRWEYNKGNYHHWRPLFLHFDTYFKTYLSCRK 89
            SE P KI  FI+ V   PL  I   L+ FRWE++KG++HHW  LF HFDT+F+ ++  RK
Sbjct: 9    SEVPTKISLFINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDTFFEKHVKVRK 68

Query: 90   DL-LSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLE-YFNLLLASTDPEILI 149
            DL + ++  E D PFPK A+LQ+LRV+++VLENC NK      E + +LLLASTD +++ 
Sbjct: 69   DLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENCTNKQFYTSYEQHLSLLLASTDADVVE 128

Query: 150  AALETLSALVKINPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCVVADEITR 209
            A L+TL+A +K    K   R       S+N  L SLAQGWG KEEGLGL SC        
Sbjct: 129  ACLQTLAAFLKRPTGKYSIRD-----ASLNLKLFSLAQGWGGKEEGLGLTSCA------- 188

Query: 210  EEGRCLFPHDVENDTNNAQYRIGSSLYFELHGCGAKSSDESSSSSSSSNSRVIHIPDLHL 269
                       E+  +    ++G +L FE +     +SDE S S      +VIH+PD+ +
Sbjct: 189  ----------TEHSCDQLFLQLGCTLLFEFY-----ASDE-SPSELPGGLQVIHVPDVSM 248

Query: 270  KKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLAFIVLVQSG 329
            + E DL +L + +  ++VPP LRF+LLTR+R+ARAF S   R+ Y+ I L AFIVLVQ+ 
Sbjct: 249  RSESDLELLNKLVIDHNVPPSLRFALLTRLRFARAFSSLATRQQYTCIRLYAFIVLVQAS 308

Query: 330  DSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSHERRILSGS 389
               + +V+FF  EPE+ NEL+ +V  E+TVP  IR L +++L A  S   + +  +L+  
Sbjct: 309  GDTENVVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVA-LSQDRTRQPTVLTAV 368

Query: 390  SISFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLHMVSSSASGNNIRSSG 449
            +   +GG+R +L  ++QKAI S+  +    SLAF EALL    + +VSSS+  + +R +G
Sbjct: 369  T---SGGHRGLLSGLMQKAIDSVICNTSKWSLAFAEALLSLVTV-LVSSSSGCSAMREAG 428

Query: 450  MVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKELGGVEILVERLQTEVHR 509
            ++PT +PL++D+DP HLHLV  AV  L+  MD+S+ A +LF++LGG++  + RL+ EV R
Sbjct: 429  LIPTLVPLIKDTDPQHLHLVSTAVHILEVFMDYSNPAAALFRDLGGLDDTIFRLKQEVSR 488

Query: 510  -------VIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIKVSLKALGVATYVP------T 569
                   ++  SG+N       +         Y+++ ++K  L+A+ + TY P       
Sbjct: 489  TEDDVKEIVCCSGSNGPEDDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYG 548

Query: 570  SSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLLHELGLPDAFLASVA 629
            S  + LP+ L +IF     FGG ++  A T+MS++IHKDPTC+  L   GL  AFL +++
Sbjct: 549  SEESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSAFLDAIS 608

Query: 630  AGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKEKYVLVM-NEAIVPL 689
              ++ S +AITC+P  + A+CLN  GL+AVK+  ALR  + IF+   Y+  + ++    L
Sbjct: 609  DEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFSSPSYLKALTSDTPGSL 668

Query: 690  ANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGNT-----AMETDSD 749
            ++ ++EL RH SSLR+ GVD+ +E++  I  +G      +S   +  T      ME D D
Sbjct: 669  SSGLDELLRHQSSLRTYGVDMFIEILNSILIIGSGMEATTSKSADVPTDAAPVPMEIDVD 728

Query: 750  DKDNNSIVTEEV--------INTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 809
            +K  +  V++E          N E  +  C+ ++  L    ++N+E C +FVE  GI+ +
Sbjct: 729  EK--SLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDTV 788

Query: 810  LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG 869
            L+L   P +  S   ++    ++ FK F+  HSA LAR  CS LR+HLKK       + G
Sbjct: 789  LQLFSLPLMPLST--SLGQSFSVAFKNFSPQHSAGLARILCSYLREHLKKTNNLLVSIEG 848

Query: 870  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ 929
            + LL   +    KI  SL  +E +L L++    +  A++++E    + +VL+++G  ++Q
Sbjct: 849  TQLLKLESAVQTKILRSLSCLEGMLSLSNFL-LKGSASVISELSAANADVLKELGITYKQ 908

Query: 930  ILWQIALL----EDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFR-QFLDPLLRRR 989
             +WQ+AL     ED K  ++  S  S++   +S     E +E   N+   ++ +P+  R 
Sbjct: 909  TIWQMALCNDTKEDEKKSVDRASDNSVS--ASSSTAERESDEDSSNALAVRYTNPVSIRS 968

Query: 990  TSG---WSVESQFFDLINLYRDL-GRAPSSLQRM--GPNGSPLLQFGANNQRRSESSDTI 1049
            +S    W    +F  ++   R + G    ++ RM  G     L  F  +    SE    +
Sbjct: 969  SSSQSIWGGHREFLSVVRSGRGVHGHTRHAIARMRGGRTRRHLESFNFD----SEIPADL 1028

Query: 1050 GASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAV 1109
              ++     K ++      +++  L+         L K     +RRR D  ++SS+SK +
Sbjct: 1029 PVTSSSHELKKKSTEVLIAEILNKLNCTLRFFFTSLVKGFTSANRRRIDGPSLSSASKTL 1088

Query: 1110 ASTLSSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLD-RPDSCNTVLLNC 1169
             + L+ + L+ +NF G+  A+G + S+S KCRY GKV+D +  +  D R   C T ++N 
Sbjct: 1089 GTALAKVFLEALNFQGYGAAAGPDTSLSLKCRYLGKVVDDITFLTFDTRRRVCFTAMVNS 1148

Query: 1170 LYGHGVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHS-WIQGPLASY 1229
             Y HG    +LTTFEATSQLL+   + P S   +   N K  E+    HS W+   L +Y
Sbjct: 1149 FYVHGTFKELLTTFEATSQLLW---KVPFSIRASSTENEKSGERNLWSHSKWLVDTLQNY 1208

Query: 1230 GRLMDHLVTSPFILSSFTK-HLLAQP----LTSGDVPFPRDAETFVKVLQSMVLKAVLPV 1289
             R +D+ V S ++LS  ++  LL QP    L+ G  P PR+ ETFV+ LQS VL+ +LP+
Sbjct: 1209 CRALDYFVNSTYLLSPTSQTQLLVQPASVDLSIGLFPVPREPETFVRNLQSQVLEVILPI 1268

Query: 1290 WTHPQFIDCSCEFITTVISIIRHIYSGV----EVKSVSSNSSARIAGP-PPNETAISTIV 1349
            W HP F DC+  F+ +V S++ HIYSGV    E +S ++  + + A P  P+E  +  IV
Sbjct: 1269 WNHPMFPDCNPNFVASVTSLVTHIYSGVVDTRENRSGATQGTNQRALPLQPDEAIVGMIV 1328

Query: 1350 EMGFSRGRAEEALRQVGSNSVELAMEWLFSHPEE-VQEDDELARALALSLGN-SELEMKE 1409
            EMGFSR RAE+ALR+VG+NSVE+AM+WLF++PE+ VQEDDELA+ALALSLGN SE    E
Sbjct: 1329 EMGFSRSRAEDALRRVGTNSVEMAMDWLFTNPEDPVQEDDELAQALALSLGNSSETPKLE 1388

Query: 1410 PVSSEVS-KQIEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNV 1469
                 V   Q E   + P  +E+++  V+L ++ +++AFP+ DL V +C++N G++R  +
Sbjct: 1389 DTEKPVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSIAFPLVDLFVTLCNRNKGEDRPKI 1448

Query: 1470 MSFLIESVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQW 1529
            + +LI+ +K   ++  S ++  L+ + H++AL+L++D   RE A Q+G+V V+  +L+ +
Sbjct: 1449 VFYLIQQLK-LVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVAVAIGILTDF 1508

Query: 1530 DSGLSFGVKVEVPKWVTAAFLAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDE----- 1589
            +        +  PK ++A  L +  +LQ + + + E  +     N GG  +  D      
Sbjct: 1509 NLKSESETDILAPKCISALLLVLSMMLQAQTRLSSEYVE----GNQGGSLVLSDSPQDST 1568

Query: 1590 --------------DKQNKLQSALGLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVL 1649
                          +    L+S  G S  Y+ +    +++ IAC  IK+R+P   M AVL
Sbjct: 1569 AALKDALSSDVAKGESNQALESMFGKSTGYLTMEESSKVLLIACGLIKQRVPAMIMQAVL 1628

Query: 1650 QLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDPQTLQQAME 1709
            QLC+ LT+SH++A+ FLE GGL+SL +LP    FPG+D++AS I+RH++EDPQTLQ AME
Sbjct: 1629 QLCARLTKSHALAIQFLENGGLSSLFNLPKKCFFPGYDTVASVIVRHLVEDPQTLQIAME 1688

Query: 1710 SEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIEMIGERPYVVL 1769
            +EIR TL  +  RH  GRV PR FL  +  VISRDPV+FM+A  S CQ+E  G   +V+L
Sbjct: 1689 TEIRQTL--SGKRH-IGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGTDFVIL 1748

Query: 1770 LKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD--SRSSRVH 1829
             K++EK K    +                +  PLG +         +K HD   + S+ H
Sbjct: 1749 TKEKEKPKVSGSE------------HGFSLNEPLGIS--------ENKLHDGSGKCSKSH 1808

Query: 1830 KKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDVAAVKGKGKAIASL 1889
            ++   NFI VI+ L++ V +F P +K          A     MD+D    K KGK+    
Sbjct: 1809 RRVPTNFIQVIDQLIDIVLSF-PGLK-----RQEGEAANLISMDVDEPTTKVKGKSKVGE 1868

Query: 1890 SEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSRPVHQRESNGGC 1949
             E      E S  LA+V FILKLL++I+LMY     V++RRDTE+   R  +  + + G 
Sbjct: 1869 PEKAELGSEKSEELARVTFILKLLSDIVLMYLHGTSVILRRDTEISQLRGSNLPDDSPG- 1928

Query: 1950 TGGIIHHILHGFIPLSRNSKKEKKV-DGDWKHKLATRGSQFLVASCVRSSEARRRIFVEV 2009
             GG+I+H++H  +P+S     EK V   +WK KL+ + S FLV  C RS+E R+RI  E+
Sbjct: 1929 NGGLIYHVIHRLLPIS----LEKFVGPEEWKEKLSEKASWFLVVLCSRSNEGRKRIINEL 1988

Query: 2010 GSILNQFIDSCNGSRP----PSSDLQAFVDLLNDMLAARTPTGSY----ITTEASATFVD 2069
              +L+ F      S      P   + AF +L+  +L   + + ++     + + + + +D
Sbjct: 1989 TRVLSVFASLGRSSSQSVLLPDKRVLAFANLVYSILTKNSSSSNFPGCGCSPDVAKSMID 2048

Query: 2070 AGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTK-----EHVHFGDSNAGKGDSSSK 2129
             G ++  T IL V+DLDHPD+PK+VT ++K+LE +T+     E +     N  K   S +
Sbjct: 2049 GGTIQCLTSILNVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKSEVPNEQKNTDSDE 2108

Query: 2130 TPDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYG-GSEAVTDDMEHDQD 2189
              D H        +      +  Q   +++   Q +P  ++Q  G    ++T  M  +  
Sbjct: 2109 RHDSHGTSTSTEVDELNQNNSSLQQVTDAVDNGQEQPQVSSQSEGERGSSLTQAMLQEMR 2168

Query: 2190 LHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDE 2249
            + G      E   M    EE  G     D +++   ++    +++D+D D+E   D+GD+
Sbjct: 2169 IEGDETILPEPIQMDFFREEIEG-----DQIEMSFHVEDRADDDVDDDMDDEGEDDEGDD 2228

Query: 2250 VDEDEDED------------EDEDE----EEQND--LEDDEVHHLPHPDTDHDDHEIDDD 2309
             D D  ED            ED ++    +E ND  +++DE     + D   D+ E D+D
Sbjct: 2229 EDADSVEDGAGVMSIAGTDVEDPEDTGLGDEYNDDMVDEDEEDEDEYNDDMVDEDEDDED 2288

Query: 2310 EFDEVLEEDDEDDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQN---ETLHVMPVE- 2369
            E+++ + ++DEDD  +   + +R  E ++G+   DH ++ GR    N   + +   P E 
Sbjct: 2289 EYNDDMVDEDEDDFHETRVIEVRWREALDGL---DHFQIVGRSGGGNGFIDDITAEPFEG 2348

Query: 2370 -----IFGSRR----QGRTTSIYNLLGRTGDTVAPSRHPLLGGPG--------------- 2429
                 +F  RR    + R  +  +   R+G  V   +HPL   P                
Sbjct: 2349 VNVDDLFALRRSLGFERRRQTGRSSFDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSI 2408

Query: 2430 ---------------LHATPFRPSENNRDMVISERSLENNSSG--LDTVFRSLRSGRHGH 2489
                           +  +P  P +       S+R     +     D     + S R G 
Sbjct: 2409 SRHSEAGSYDVAQFYMFDSPVLPFDQVPVDPFSDRLGGGGAPPPLTDYSVVGMDSSRRGV 2468

Query: 2490 RLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTP-EKLSELDANAEPDNRDGNGQIQ 2549
              + W +       S +  I Q +EE  ++ LR   P + + E + N           + 
Sbjct: 2469 GDSRWTDVGHPQPSSLSASIAQLIEEHFITNLRASAPVDTVVERETNTTEVQEQQQPDV- 2528

Query: 2550 TSEPSGRSETIV-------ENSGQH---GNDNLPPPVAINSSDGTNSRPAASESLQGTQV 2609
               PS  SET++       E S +H    N+ +  P+ +NS+     R    E       
Sbjct: 2529 --PPSVGSETVLGDGNEGGEQSEEHELLNNNEVMHPLPLNSTPNEIDRMEVGEG------ 2588

Query: 2610 IQQSQPVEMQFEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQG 2669
                 P+E           D EAV   S   G +    ++++ V        D   + Q 
Sbjct: 2589 --GGAPIEQV---------DREAVHLISSAQGQSDTSGIQNVSVTAIPPPVDDPDSNFQP 2648

Query: 2670 SADRMSLGDSQAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQT 2729
            S D     D                + L G +  L  +    +  + E  +EG +  +  
Sbjct: 2649 SVDVDMSSDGAEGNQS------VQPSPLDGDNNELSSMEATQDVRNDEQVDEGSLDGRAP 2708

Query: 2730 NSDTGLGAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIR 2789
                 + AIDP FL+ALPE+LRAEVL++QQ Q VQPP+ EP +  DIDPEFLAALPP+I+
Sbjct: 2709 E----VNAIDPTFLEALPEDLRAEVLASQQAQSVQPPTYEPPSVDDIDPEFLAALPPEIQ 2768

Query: 2790 AEVLAQQQAQRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVA 2849
             EVLAQQ+AQR  + Q+ +GQPV+MD  SIIAT P+DLREEVLLTSS+A+LA L P L+A
Sbjct: 2769 REVLAQQRAQR--MLQQSQGQPVDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLA 2828

Query: 2850 EANMLRERFAHRYHNRS-LFGMHPR--NRRGEITRRVEGISGLDRTGGSISSRRSL---- 2909
            EA MLR+R    Y  RS +FG   R  NRR  +  R   ++G++R  G    +R +    
Sbjct: 2829 EAQMLRDRAMRHYQARSRVFGSSHRLNNRRNGLGYR---LTGMERGVGVTIGQRDVSSSA 2888

Query: 2910 -GSRLIEAEGAPLVDTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMD 2969
             G ++ E EG PLV+ DAL ++IRLLR+ QPL KG LQRLLLNLCAH+ TR +LV++L+D
Sbjct: 2889 DGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRLLLNLCAHSFTRANLVQLLLD 2948

Query: 2970 VLLFDRRKPANQSNTTELS----YRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLA 3029
            ++     +P  ++  +EL+     RLY CQ NV+Y R    +G PPLV RRVLE LTYLA
Sbjct: 2949 MI-----RPEMETLPSELALTNPQRLYGCQLNVVYGRSQLLNGLPPLVFRRVLEVLTYLA 3008

Query: 3030 RNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSL 3089
             NH  VA +L    +F  +L    +  +   K     D  +++        + + + L L
Sbjct: 3009 TNHSAVADMLF---YFDSSLLSQLSSRKGKEKVTHETDSRDLE--------IPLVVFLKL 3068

Query: 3090 LNQPLYLRSIAHLEQLLNLLEVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQ 3149
            LN+P  L+S +HL  ++ LL+V++  A S+        +        PE  L +  V  +
Sbjct: 3069 LNRPQLLQSTSHLALVMGLLQVVVYTAASR-------IEGWSPSSGVPE-KLENKPVGEE 3128

Query: 3150 SSSVSSGVGTSSKLGSYFKMAASAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTY 3209
            +SS +     S       +++ +      ++++I   LP+++L  LC LL  EGLSD  Y
Sbjct: 3129 ASSETQKDAES-------ELSVARRKNCAELYNIFLQLPQSDLCNLCMLLGYEGLSDKIY 3188

Query: 3210 ALVGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAMDELHMFGEAVK-ALSISSSDGAA 3269
            +L GEV+KKL  +   H + F  ELSE    L+ S +  L       K + +  S  GA+
Sbjct: 3189 SLAGEVLKKLAAVDVTHRKFFTKELSELASGLSSSTVRVLATLSTTQKMSQNTCSMAGAS 3248

Query: 3270 ILRVLQALSSLVALLVEKRKDSSILPEKE-HASALTLVCDINAALEPLWLELSTCIGKIE 3329
            ILRVLQ LSSL + +     DS++  +KE       ++  +  ALEPLW EL  CI   E
Sbjct: 3249 ILRVLQVLSSLTSTI----DDSNVGTDKETDQEEQNIMQGLKVALEPLWQELGQCISMTE 3308

Query: 3330 SYSDSSPDTLVSFRTPTSKPTGVTP--PLPAGSQNILPYIESFFVVCEKLHPAQPGSDQE 3389
               D +  T  S   P     G++P   L  G+Q++LP IE+FFV+CEK+    P   Q+
Sbjct: 3309 LQLDHTAAT--SNVNPGDHVLGISPTSSLSPGTQSLLPLIEAFFVLCEKIQ--TPSMLQQ 3368

Query: 3390 LNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKS 3449
                T  EV+++       +T V +QK  +  + F +F EKHR+LLN+F+RQNP LLEKS
Sbjct: 3369 DATVTAGEVKESSTHGSSSKTIVDSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPSLLEKS 3428

Query: 3450 FSPMLKVPRFIDFDNKRSHFRSRIKHQHDHH--SPLRISVRRAYILEDSYNQLRMRSTQD 3509
            FS MLK PR IDFDNK+++FRSRI+HQHD H   PLRISVRRAY+LEDSYNQLRMRS QD
Sbjct: 3429 FSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQD 3488

Query: 3510 LKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGALLFTTVGNDSTFQPNPNSGYQTE 3569
            LKGRL V FQGEEGIDAGGL+REWYQ+LSRVIFDKGALLFTTVGND+TFQPNPNS YQTE
Sbjct: 3489 LKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTE 3548

Query: 3570 HLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLE 3629
            HLSYFKFVGR+V KAL+DGQLLDV+FTRSFYKHILGVKVTYHDIEA+DPDYYKNLKW+LE
Sbjct: 3549 HLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLE 3608

Query: 3630 NDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLT 3664
            ND+SD+L LTFS+DADEEK ILYEKTEVTDYEL PGGRN +VTEE K++YVDLV +H LT
Sbjct: 3609 NDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILT 3657

BLAST of CmoCh12G001650 vs. ExPASy Swiss-Prot
Match: Q7TMY8 (E3 ubiquitin-protein ligase HUWE1 OS=Mus musculus OX=10090 GN=Huwe1 PE=1 SV=5)

HSP 1 Score: 544.7 bits (1402), Expect = 8.2e-153
Identity = 1041/4531 (22.98%), Postives = 1736/4531 (38.31%), Query Frame = 0

Query: 27   KLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFR-WEYNKGNYHHWRPLFLHFDTYF---- 86
            K  +E P   +A IDK+  C    + + L   + W   K   +HW  L   FD       
Sbjct: 10   KTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAG 69

Query: 87   KTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLEYFNLLLAST 146
            +T  +    L+ D+  ++     K  +L +L    +++E   ++     +E+   LLAS+
Sbjct: 70   QTVENMSWMLVCDRPEKEQL---KMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASS 129

Query: 147  DPEILIAALETLSALVK-INPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCV 206
            D ++++A L  L    K  N     G  K      + + L  LA+ WG KE G GL  C 
Sbjct: 130  DMQVVLAVLNLLYVFSKRSNYITRLGSDKRT---PLLTRLQHLAESWGGKENGFGLAEC- 189

Query: 207  VADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHG-CGAKSSDESSSSSSSSNSRV 266
                  R+     +P               ++L+FE +   GA+   E  ++S++ +   
Sbjct: 190  -----CRDLQMLKYPPS------------ATTLHFEFYADPGAEVKIEKRTTSNTLH--Y 249

Query: 267  IHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLA 326
            IHI  L    E    +++   ++Y +P + +  L T IR A  F +   R    +  L A
Sbjct: 250  IHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHA 309

Query: 327  FIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSH 386
              +LV S    +   +   N      EL+ +++  +     I+  ++R L +       H
Sbjct: 310  ISILVYSNALQESANSILYN--GLIEELVDVLQITDKQLMEIKAASLRTLTS-----IVH 369

Query: 387  ERRILSGSSI-------SFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLH 446
              R    SSI       S+ G    ++ N +Q  I     S DP    F  AL  F L H
Sbjct: 370  LERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMI---DPSMDPYPHQFATALFSF-LYH 429

Query: 447  MVSSSASGNNIRSSGMVPTFLPLLE--DSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKE 506
            + S  A G  + S GM+   L +++    +   +  V  AV+ +  + +   +A   F+ 
Sbjct: 430  LASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAA---FQS 489

Query: 507  LGGVEILVERLQTEVHR-------VIGLSGNNIDSMIIGESSRCNDDQLY---NQKRMIK 566
              G+ I + RL+ EV         VI        +   GE    + D +     +  ++K
Sbjct: 490  HSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLK 549

Query: 567  VSLKALGVATYVPTSS-------TNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKD 626
              L  L  A   P  S         +LP  L  I  N + +G  ++  A  +++  + ++
Sbjct: 550  SMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQE 609

Query: 627  PTCYPLLHELGLPDAFLASVAAGILPSPKAIT-CVPNGIGAICLNARGLEAVKETLALRF 686
            P+    L + GL D  L ++    +P+ + +   +PN   A+CLNARGL++  +      
Sbjct: 610  PSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFER 669

Query: 687  LIDIFTKEKYVLVM---------NEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKIT 746
            L  +     Y+  M          +    L +AV+EL RH  +L++     I++++E+I 
Sbjct: 670  LFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEIC 729

Query: 747  SLGDKDP-----VDSSGKLNG----------NTAMETDSDDKD---------------NN 806
            +LG +DP       S  K +G          + A E  S+D++               N 
Sbjct: 730  NLG-RDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNE 789

Query: 807  SIVTEEVINTEQVVQL----CIFHLMVLVHRTMENSET---CRIFVENSGIEALLKLLLR 866
            +   ++V+ TE+ + +     I ++M  V   + N+ T   C+ FV   G+  L+ +L  
Sbjct: 790  TEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGL 849

Query: 867  PTIAQSANGTIALHSTM-VFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSGSFLLD 926
            P +      + A  +   V K        P            L++ L   DL+  S  L+
Sbjct: 850  PNLPIDFPTSAACQAVAGVCKSILTLSHEPKV----------LQEGLLQLDLILSS--LE 909

Query: 927  PRTTPDEKIFSSLFLVEFLLF--LADSKDNRWVAALL----------------------- 986
            P   P E    S+ L E      +AD+  +     LL                       
Sbjct: 910  PLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSE 969

Query: 987  ------TEFGNE-SKNVLEDIGRVHRQILWQIALLEDVKPELEDESTGSITELQNSEVHT 1046
                   ++G++   +VL  + +++  ++W+  +L  +       S  S  E   +++  
Sbjct: 970  IRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSL---CTPNSLPSGCEFGQADMQK 1029

Query: 1047 NEIEEQRFNSFRQFLDPLLRRRTSGWSVESQFFDLINLYRDLGRAPSSLQRMGPNGSPLL 1106
               ++++  + +        +  +  S+++    L+            ++  G   +P+ 
Sbjct: 1030 LVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLL----------EGIELDGDTLAPM- 1089

Query: 1107 QFGANNQRRSESSDTIGASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLL-- 1166
                        +D   +S+ K  SK     A+    ++ L   ++ L + L ++  L  
Sbjct: 1090 -----------ETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLV 1149

Query: 1167 ------PSRRR------DDVVNVSSSSKAVASTLSSLVLDHMNFVGHVNASGSEGSISTK 1226
                  P R+R            + ++++ AS L+ L+   +++           +   +
Sbjct: 1150 KLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSW----QPPPYTPTPRFR 1209

Query: 1227 CRYFGKVIDFVDGILLDRPDSCNTVLLN---CLYGHGVVSSVLTTFEATSQLLFTINRAP 1286
              +F   + F   +L D       ++L    C  GH  +      FE  +  L    + P
Sbjct: 1210 LTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNAL------FETFNWALSMGGKVP 1269

Query: 1287 SSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHLVTSPFILSSFTKHLLAQPLTSG 1346
             S        L+  +  D    ++   L     L++ +V    +L S   H L   L  G
Sbjct: 1270 VS------EGLEHSDLPDGTGEFLDAWL----MLVEKMVNPTTVLES--PHSLPAKLPGG 1329

Query: 1347 DVPFPR-DAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVISIIRHIYSGVEV--- 1406
               FP+  A  F+ V Q      +  +W              ++++I+ HI  G  V   
Sbjct: 1330 VQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRE 1389

Query: 1407 ---KSVSSNSSARIAG---------PPPNETAISTIVEMGFSRGRAEEALRQVGSNSVEL 1466
               K    +     AG         P  N+  +  +++MGF+R  A EAL  + ++++E 
Sbjct: 1390 RLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEAL--LNTSTMEQ 1449

Query: 1467 AMEWLFSHPEEV----------QEDDELARALALSLGN-----------SELEMKEPVSS 1526
            A E+L +HP  +           E+D++ RA+A+SLG             E+  ++    
Sbjct: 1450 ATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEE 1509

Query: 1527 EVSKQIEESVQLPC------------------TEELLSTCVRLLRAKETLAFPVRDLLVM 1586
              +++ +E  +  C                  T+ +L  C  LL       + V DL++ 
Sbjct: 1510 RKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMT 1569

Query: 1587 ICSQNDGQNRSNVMSFLIESV--------KGCDKISDSGNSTT----------------- 1646
               +N    R  ++  ++  V        K    ++ S   T                  
Sbjct: 1570 AIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLA 1629

Query: 1647 -----LSALFHVIAL----------ILN-------------------------------- 1706
                 L+ LF  + L          ILN                                
Sbjct: 1630 TRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVL 1689

Query: 1707 ---------------------------------DDTVAREAAYQNGLVTVSSNLLSQWDS 1766
                                             DD   R  +Y     + +S + S W S
Sbjct: 1690 LLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYS---ASNNSTIDSAWKS 1749

Query: 1767 G--------------LSFGVKVEV------PKWVTAAFLAIDRLLQEEKKFN-------- 1826
            G              + F   V+V       + V    L + RL +  K  N        
Sbjct: 1750 GETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTL 1809

Query: 1827 --PEIADQLKRDNVGGDTLTIDEDKQNKLQSAL-----------GLSPKYIDVGNQKELI 1886
               +  D   ++N G D     E    + +++L           GL+   + V     LI
Sbjct: 1810 EESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTV-----LI 1869

Query: 1887 EIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSI 1946
                S +   +  +T+HA L+LC  LTR H  A+ F E      +L+L  +S F GF  +
Sbjct: 1870 RACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPL 1929

Query: 1947 ASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPR------NFLLG-LNSVIS 2006
             + ++RHI+EDP TL+  ME  +R    +      +G V+        N++L  L     
Sbjct: 1930 VTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAAC 1989

Query: 2007 RDPVIFMRATQSVCQIEMIGERPYVVLLKDREKDKSKEKDRDK-EKSMEKEKTQNHDVKV 2066
            R+P IF        +I +   R           D   E  R K   +++  KT       
Sbjct: 1990 RNPDIFTEVANCCIRIALPAPRG-----SGTASDDEFENLRIKGPNAVQLVKT------T 2049

Query: 2067 PLGNAN-PATAGNVRSKFHDSRSSRVHKKSSQNFINVIEL---LLESVYTFIPPMKDDVT 2126
            PL  ++ P     ++   +D  ++     + +             + V   +  M DDV 
Sbjct: 2050 PLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVY 2109

Query: 2127 VELTSGAQASSDMDI--------DVAAVKG-------------------------KGKAI 2186
             +  S  + SSD D          V A  G                         K K  
Sbjct: 2110 QQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEASTPEETREGKKDKEG 2169

Query: 2187 ASLSEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSRPVHQRESN 2246
               SE+     + S  L     IL+LL E++  Y     ++      V  S  + +    
Sbjct: 2170 DRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKE---- 2229

Query: 2247 GGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVRSSEARRRIFV 2306
              C+  ++  +L   +P ++N+ ++K      +  LA+      +A+    ++A+  +  
Sbjct: 2230 -DCS--VLAFVLDHLLPHTQNA-EDKDTPALARLFLAS------LAAAGSGTDAQVALVN 2289

Query: 2307 EVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTGSYITTEASAT--------- 2366
            EV + L + +     S    + LQA + +++ ++ +   T S+ ++  + T         
Sbjct: 2290 EVKAALGRALAMAE-STEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNII 2349

Query: 2367 --FVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALE----LVTKEHVHFG-------- 2426
              F+  GLV    ++   LDL  P+    V   +K LE    +V +    FG        
Sbjct: 2350 RLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKN 2409

Query: 2427 ----DSNAGKGDSSSKTPDHHQPGGENIGETPRAMETVSQSNHESIPAE----------- 2486
                D+     DSSS   D  +PG   + E    +     ++ + +  E           
Sbjct: 2410 KSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQ 2469

Query: 2487 -QIEPYNANQIYGGSEAVTDDMEHDQDLHG----VFGANAEDECMH-VTPEEARGLENGI 2546
             ++      Q+    E + D++       G    +   + EDE    V  +EA    +  
Sbjct: 2470 PEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQA 2529

Query: 2547 DTVDIQIEIQPHV--PENLDEDDDEEMSGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHH 2606
             T+    E   ++  PE+ +E   EE S    ++ D+ +DE+E+E+E+E++D EDDE   
Sbjct: 2530 STLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEE 2589

Query: 2607 LPHPDTDH-DDHEIDDD----------EFDEVLEEDDEDDEDDE---------------- 2666
                D D   + E+D+D           F+    EDD   E D                 
Sbjct: 2590 GDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIP 2649

Query: 2667 ----------DGVLLRLEEGINGINVFDHVEVFGRDTSQ--NETLHVMPVEIFGSRRQGR 2726
                      D   L L  G +   +   +    R   Q    T H + V   G+R+   
Sbjct: 2650 TTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNP 2709

Query: 2727 TTSIYNLLG--RTGDTVAPS----------RHPLLGGPGLHATPFRPSENNRDMVISERS 2786
               +  LLG     D +  S             L+G   +H       E   D    + +
Sbjct: 2710 PLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQST 2769

Query: 2787 LENNSSGLDTVFRSLRSGRHGHRLNLWANDIQHNGGSNNGV---------IPQGLEELLV 2846
              + +  L ++  +L   R      +   +  H+  S   V           + LEE   
Sbjct: 2770 ATSQAGTLSSIPTALT--RWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERRE 2829

Query: 2847 SQLRRPTPEKLSELDANAE-PDNRDGNGQI----------------------------QT 2906
             + ++   E+   +D   E  +NRD + Q                              T
Sbjct: 2830 KRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSST 2889

Query: 2907 SEPSGRS-ETIVENSGQHGNDNLPPPVAIN---------SSDGTNS----RPAASESLQG 2966
              P+  S ET+           LPPP ++          + +  NS     P   E L  
Sbjct: 2890 DAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGP 2949

Query: 2967 TQVIQQSQPVEMQF--------------EHNDAAVRDVEAVSQESGGSGATFGESLRSLD 3026
            T+   +++  +M+               E +DA    + AVS +  GS      SL S  
Sbjct: 2950 TRPSGEAETTQMELSPAPTITSLSPERAEDSDA----LTAVSSQLEGSPMD-TSSLASCT 3009

Query: 3027 VEIGSYDGHDDGGDRQGSADRMSLGDSQ---AARMRRSNVSF------------TNSASL 3086
            +E    D    G   Q +A   + GD+    A    R +VS              +S S 
Sbjct: 3010 LEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESS 3069

Query: 3087 SGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLG-----AIDPAFLDALPEELRA 3146
            S RD+++     +S   SR   EE          D   G      +DP+FL ALP+++R 
Sbjct: 3070 STRDSAV----AISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRR 3129

Query: 3147 EVLSTQQGQVVQPPSNEPQNSG-------------DIDPEFLAALPPDIRAEVLAQQQA- 3206
            EVL  Q G  ++PP+    +S              ++ PEFLAALPP I+ EVLAQQ+A 
Sbjct: 3130 EVLQNQLG--IRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAE 3189

Query: 3207 -QRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR-- 3266
             QR  L+Q        MD V+ I T PSDLR  VL    D++LA + P + AEA  LR  
Sbjct: 3190 QQRRELAQNASSD-TPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRRE 3249

Query: 3267 -ERFAHRYHNRSLFGMHPRN------RRGEITRRVEGISGLDRT------------GGSI 3326
             E    +  +  LFG    +      R    T R+ G  G+  T            GGS 
Sbjct: 3250 QEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS 3309

Query: 3327 SSRRSLGSR---LIEAEGAPLVDTDALHAMIRLLRVVQP-LYKGQLQRLLLNLCAHNETR 3386
            S  R  GS    L+   G  L+D +AL  ++ LL V +P L   +L R+L NLC H +TR
Sbjct: 3310 SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTR 3369

Query: 3387 TSLVKILMDVL-----------------LFDRRKPANQS--------------------N 3446
              +++ L+ +L                   +R K +++S                    +
Sbjct: 3370 HWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKS 3429

Query: 3447 TTELSY------RLYACQRNVIYSRPS-----------------FFDGAPPLVSRRVLET 3506
            + +LS+          C+ N+   + S                     A P+V R VL+T
Sbjct: 3430 SNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDT 3489

Query: 3507 LTYLARNHP-HVAKILLQ----------------------------CKFFRPTLQESENV 3566
            L  LA+  P H  +   +                            C  F   L + +N+
Sbjct: 3490 LIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNM 3549

Query: 3567 N-----QDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLE 3626
            N     ++  K+V      E +  P S     +  L+++L+ P+  RS    E+LL LL 
Sbjct: 3550 NVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLS 3609

Query: 3627 VI--------IDNAESKSHLSDQPTQST-------------------------------- 3663
            +I        +   ++ S  S   T +T                                
Sbjct: 3610 LISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPA 3669

BLAST of CmoCh12G001650 vs. ExPASy Swiss-Prot
Match: Q7Z6Z7 (E3 ubiquitin-protein ligase HUWE1 OS=Homo sapiens OX=9606 GN=HUWE1 PE=1 SV=3)

HSP 1 Score: 544.3 bits (1401), Expect = 1.1e-152
Identity = 1038/4510 (23.02%), Postives = 1735/4510 (38.47%), Query Frame = 0

Query: 27   KLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFR-WEYNKGNYHHWRPLFLHFDTYF---- 86
            K  +E P   +A IDK+  C    + + L   + W   K   +HW  L   FD       
Sbjct: 10   KTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAG 69

Query: 87   KTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLEYFNLLLAST 146
            +T  +    L+ D+   +     K  +L +L    +++E   ++     +E+   LLAS+
Sbjct: 70   QTVENMSWMLVCDRPEREQL---KMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASS 129

Query: 147  DPEILIAALETLSALVK-INPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCV 206
            D ++++A L  L    K  N     G  K      + + L  LA+ WG KE G GL  C 
Sbjct: 130  DMQVVLAVLNLLYVFSKRSNYITRLGSDKRT---PLLTRLQHLAESWGGKENGFGLAEC- 189

Query: 207  VADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHG-CGAKSSDESSSSSSSSNSRV 266
                       C   H ++   +       ++L+FE +   GA+   E  ++S++ +   
Sbjct: 190  -----------CRDLHMMKYPPS------ATTLHFEFYADPGAEVKIEKRTTSNTLH--Y 249

Query: 267  IHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLA 326
            IHI  L    E    +++   ++Y +P + +  L T IR A  F +   R    +  L A
Sbjct: 250  IHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHA 309

Query: 327  FIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSH 386
              +LV S    +   +   N      EL+ +++  +     I+  ++R L +       H
Sbjct: 310  ISILVYSNALQESANSILYN--GLIEELVDVLQITDKQLMEIKAASLRTLTS-----IVH 369

Query: 387  ERRILSGSSI-------SFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLH 446
              R    SSI       S+ G    ++ N +Q  I     S DP    F  AL  F L H
Sbjct: 370  LERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMI---DPSMDPYPHQFATALFSF-LYH 429

Query: 447  MVSSSASGNNIRSSGMVPTFLPLLE--DSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKE 506
            + S  A G  + S GM+   L +++    +   +  V  AV+ +  + +   +A   F+ 
Sbjct: 430  LASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAA---FQS 489

Query: 507  LGGVEILVERLQTEVHR-------VIGLSGNNIDSMIIGESSRCNDDQLY---NQKRMIK 566
              G+ I + RL+ EV         VI       ++   GE    + D +     +  ++K
Sbjct: 490  HSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLK 549

Query: 567  VSLKALGVATYVPTSS-------TNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKD 626
              L  L  A   P  S         +LP  L  I  N + +G  ++  A  +++  + ++
Sbjct: 550  SMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQE 609

Query: 627  PTCYPLLHELGLPDAFLASVAAGILPSPKAIT-CVPNGIGAICLNARGLEAVKETLALRF 686
            P+    L + GL D  L ++    +P+ + +   +PN   A+CLNARGL++  +      
Sbjct: 610  PSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFER 669

Query: 687  LIDIFTKEKYVLVM---------NEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKIT 746
            L  +     Y+  M          +    L +AV+EL RH  +L++     I++++E+I 
Sbjct: 670  LFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEIC 729

Query: 747  SLGDKDP-----VDSSGKLNG----------NTAMETDSDDKD---------------NN 806
            +LG +DP       S  K +G          + A E  S+D++               N 
Sbjct: 730  NLG-RDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNE 789

Query: 807  SIVTEEVINTEQVVQL----CIFHLMVLVHRTMENSET---CRIFVENSGIEALLKLLLR 866
            +   ++V+ TE+ + +     I ++M  V   + N+ T   C+ FV   G+  L+ +L  
Sbjct: 790  TEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGL 849

Query: 867  PTI---------AQSANG------TIALHSTMVFKGFTQHHS-----APLARAFCSSLRD 926
            P +          Q+  G      T++    ++ +G  Q  S      PL R   S    
Sbjct: 850  PNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGS 909

Query: 927  HLKKVLTGFDLVSGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNE 986
             L + L     V+ + L    T     + ++   +   +       +   +  + ++G++
Sbjct: 910  VLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQ 969

Query: 987  -SKNVLEDIGRVHRQILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFR 1046
               +VL  + +++  ++W+  +L  +       S  S  E   +++     ++++  + +
Sbjct: 970  LGLSVLSKLSQLYCSLVWESTVLLSL---CTPNSLPSGCEFGQADMQKLVPKDEKAGTTQ 1029

Query: 1047 QFLDPLLRRRTSGWSVESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQRRSES 1106
                    +  +  S+++    L             L+ +G +G  L             
Sbjct: 1030 GGKRSDGEQDGAAGSMDASTQGL-------------LEGIGLDGDTLAPM---------E 1089

Query: 1107 SDTIGASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLL--------PSRRR- 1166
            +D   AS+ K  SK     A+    ++ L   ++ L + L ++  L        P R+R 
Sbjct: 1090 TDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRR 1149

Query: 1167 -----DDVVNVSSSSKAVASTLSSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVD 1226
                       + ++++ AS L+ L+   +++           +   +  +F   + F  
Sbjct: 1150 SHHAASTTTAPTPAARSTASALTKLLTKGLSW----QPPPYTPTPRFRLTFFICSVGFTS 1209

Query: 1227 GILLDRPDSCNTVLLN---CLYGHGVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLK 1286
             +L D       ++L    C  GH  +      FE  +  L    + P S        L+
Sbjct: 1210 PMLFDERKYPYHLMLQKFLCSGGHNAL------FETFNWALSMGGKVPVS------EGLE 1269

Query: 1287 QDEKTDNDHSWIQGPLASYGRLMDHLVTSPFILSSFTKHLLAQPLTSGDVPFPR-DAETF 1346
              +  D    ++   L     L++ +V    +L S   H L   L  G   FP+  A  F
Sbjct: 1270 HSDLPDGTGEFLDAWL----MLVEKMVNPTTVLES--PHSLPAKLPGGVQNFPQFSALRF 1329

Query: 1347 VKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVISIIRHIYSGVEV--------------- 1406
            + V Q      +  +W              ++++I+ HI  G  V               
Sbjct: 1330 LVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGE 1389

Query: 1407 KSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVGSNSVELAMEWLFSHPEEV 1466
            +            P  N+  +  +++MGF+R  A EAL  + ++++E A E+L +HP  +
Sbjct: 1390 EDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEAL--LNTSTMEQATEYLLTHPPPI 1449

Query: 1467 ----------QEDDELARALALSLGN-----------SELEMKEPVSSEVSKQIEESVQL 1526
                       E+D++ RA+A+SLG             E+  ++      +++ +E  + 
Sbjct: 1450 MGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEA 1509

Query: 1527 PC------------------TEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSN 1586
             C                  T+ +L  C  LL       + V DL++    +N    R  
Sbjct: 1510 KCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDM 1569

Query: 1587 VMSFLIESV--------KGCDKISDSGNSTT----------------------LSALFHV 1646
            ++  ++  V        K    ++ S   T                       L+ LF  
Sbjct: 1570 ILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEE 1629

Query: 1647 IAL----------ILN-------------------------------------------- 1706
            + L          ILN                                            
Sbjct: 1630 LKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAIS 1689

Query: 1707 ---------------------DDTVAREAAYQNGLVTVSSNLLSQWDSG----------- 1766
                                 DD   R  +Y     + +S + S W SG           
Sbjct: 1690 SKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYS---ASNNSTIDSAWKSGETSVRFTAGRR 1749

Query: 1767 ---LSFGVKVEV------PKWVTAAFLAIDRLLQEEKKFNPEIADQL----------KRD 1826
               + F   V+V       + V    L + RL +  K  N +  ++           +++
Sbjct: 1750 RYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKE 1809

Query: 1827 NVGGDTLTIDEDKQNKLQSAL-----------GLSPKYIDVGNQKELIEIACSCIKKRLP 1886
            N G DT    E    + +++L           GL+   + V     LI    S +   + 
Sbjct: 1810 NKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTV-----LIRACVSMLGVPVD 1869

Query: 1887 CETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDP 1946
             +T+HA L+LC  LTR H  A+ F E      +L+L  +S F GF  + + ++RHI+EDP
Sbjct: 1870 PDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDP 1929

Query: 1947 QTLQQAMESEIRHTLITAMNRHPNGRVTPR------NFLLG-LNSVISRDPVIFMRATQS 2006
             TL+  ME  +R    +      +G V+        N++L  L     R+P IF      
Sbjct: 1930 CTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANC 1989

Query: 2007 VCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNV 2066
              +I +   R       D E +  + K  +  + ++    +   + V      P T   V
Sbjct: 1990 CIRIALPAPRGSGT-ASDDEFENLRIKGPNAVQLVKTTPLKPSPLPV-----IPDTIKEV 2049

Query: 2067 RSKFHDSRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDI-- 2126
                 ++ ++    + +        ++ + V   +  M DDV  +  S  + SSD D   
Sbjct: 2050 IYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQS 2109

Query: 2127 ------DVAAVKG-------------------------KGKAIASLSEDDANSQEASASL 2186
                   V A  G                         K K     SE+     + S  L
Sbjct: 2110 GFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPL 2169

Query: 2187 AKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSRPVHQRESNGGCTGGIIHHILHGFIP 2246
                 IL+LL E++  Y     ++      V  S  + +      C+  ++  +L   +P
Sbjct: 2170 MPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKE-----DCS--VLAFVLDHLLP 2229

Query: 2247 LSRNSKKEKKVDGDWKHKLATRGSQFLVASCVRSSEARRRIFVEVGSILNQFIDSCNGSR 2306
             ++N+ ++K      +  LA+      +A+    ++A+  +  EV + L + +     S 
Sbjct: 2230 HTQNA-EDKDTPALARLFLAS------LAAAGSGTDAQVALVNEVKAALGRALAMAE-ST 2289

Query: 2307 PPSSDLQAFVDLLNDMLAARTPTGSYITTEASAT-----------FVDAGLVRSFTQILK 2366
               + LQA + +++ ++ +   T S+ ++  + T           F+  GLV    ++  
Sbjct: 2290 EKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPH 2349

Query: 2367 VLDLDHPDSPKVVTGVIKALE----LVTKEHVHFG------------DSNAGKGDSSSKT 2426
             LDL  P+    V   +K LE    +V +    FG            D+     DSSS  
Sbjct: 2350 SLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQ 2409

Query: 2427 PDHHQPGGENIGETPRAMETVSQSNHESIPAE------------QIEPYNANQIYGGSEA 2486
             D  +PG   + E    +     ++ + +  E            ++      Q+    E 
Sbjct: 2410 QDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELED 2469

Query: 2487 VTDDMEHDQDLHG----VFGANAEDECMH-VTPEEARGLENGIDTVDIQIEIQPHV--PE 2546
            + D++       G    +   + EDE    V  +EA    +   T+    E   ++  PE
Sbjct: 2470 LIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPE 2529

Query: 2547 NLDEDDDEEMSGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDH-DDHEIDDD 2606
            + +E   EE S    ++ D+ +DE+E+E+E+E++D EDDE       D D   + E+D+D
Sbjct: 2530 DEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDED 2589

Query: 2607 ----------EFDEVLEEDDEDDEDDE--------------------------DGVLLRL 2666
                       F+    EDD   E D                           D   L L
Sbjct: 2590 YPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTL 2649

Query: 2667 EEGINGINVFDHVEVFGRDTSQ--NETLHVMPVEIFGSRRQGRTTSIYNLLG--RTGDTV 2726
              G +   +   +    R   Q    T H + V   G+R+      +  LLG     D +
Sbjct: 2650 GSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADIL 2709

Query: 2727 APS----------RHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRS 2786
              S             L+G   +H       E   D    + +  + +  L ++  +L  
Sbjct: 2710 QLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALT- 2769

Query: 2787 GRHGHRLNLWANDIQHNGGSNNGV-IPQGLEELLVSQLRRPTPEKLSEL---------DA 2846
             R      +   +  H+  S   V I   LE L   +L     ++  +L           
Sbjct: 2770 -RWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKG 2829

Query: 2847 NAEPDNRDGNGQIQTSE-----------------------------PSGRSETIVENSGQ 2906
              + +NRD + Q   S+                              S   ET+      
Sbjct: 2830 KEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSS 2889

Query: 2907 HGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEM------------QFEHND 2966
                 LP P A+         P A E    TQ++   +P E+            Q E + 
Sbjct: 2890 QQQPTLPTPPALGEVPQELQSP-AGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSP 2949

Query: 2967 AAV------------RDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSAD 3026
            A                + AVS +  GS      SL S  +E    D    G   Q  A 
Sbjct: 2950 APTITSLSPERAEDSDALTAVSSQLEGSPMD-TSSLASCTLEEAVGDTSAAGSSEQPRAG 3009

Query: 3027 RMSLGDS---------------QAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSRE 3086
              + GD+               ++A+    +    +S S S RD+++     +S   SR 
Sbjct: 3010 SSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAV----AISGADSRG 3069

Query: 3087 ADEEGPVGEQQTNSDTGLG-----AIDPAFLDALPEELRAEVLSTQQGQVVQPP------ 3146
              EE          D   G      +DP+FL ALP+++R EVL  Q G  ++PP      
Sbjct: 3070 ILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLG--IRPPTRTAPS 3129

Query: 3147 --SNEPQNSG-----DIDPEFLAALPPDIRAEVLAQQQA--QRLHLSQELEGQPVEMDTV 3206
              S+ P   G     ++ PEFLAALPP I+ EVLAQQ+A  QR  L+Q        MD V
Sbjct: 3130 TNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSD-TPMDPV 3189

Query: 3207 SIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR---ERFAHRYHNRSLFGMHPRN 3266
            + I T PSDLR  VL    D++LA + P + AEA  LR   E    +  +  LFG    +
Sbjct: 3190 TFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTS 3249

Query: 3267 ------RRGEITRRVEGISGLDRT------------GGSISSRRSLGSR---LIEAEGAP 3326
                  R    T R+ G  G+  T            GGS S  R  GS    L+   G  
Sbjct: 3250 ALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRL 3309

Query: 3327 LVDTDALHAMIRLLRVVQP-LYKGQLQRLLLNLCAHNETRTSLVKILMDVL--------- 3386
            L+D +AL  ++ LL V +P L   +L R+L NLC H +TR  +++ L+ +L         
Sbjct: 3310 LLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELC 3369

Query: 3387 -------------------------------LFDRRKPANQSNTTELSYRLYA---CQRN 3446
                                           L  + +  + +  + LS  + A   C+ N
Sbjct: 3370 IETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTN 3429

Query: 3447 VIYSRPS-----------------FFDGAPPLVSRRVLETLTYLARNHP-HVAKILLQ-- 3506
            +   + S                     A P+V R VL+TL  LA+  P H  +   +  
Sbjct: 3430 IFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKET 3489

Query: 3507 -------------------------CKFFRPTLQESENVN-----QDCGKAVMAVDRNEI 3566
                                     C  F   L + +N+N     ++  K+V      E 
Sbjct: 3490 NCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEG 3549

Query: 3567 DCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVI--------IDNAESKS--- 3626
            +  P S     +  L+++L+ P+  RS    E+LL LL +I        +  A++ S   
Sbjct: 3550 ETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSG 3609

Query: 3627 -----------------HLSDQPTQSTVEQP--AAPE-----------VSLSDAEVNGQS 3663
                               S  PT  T   P  +AP            V+ S       +
Sbjct: 3610 ASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTT 3669

BLAST of CmoCh12G001650 vs. ExPASy Swiss-Prot
Match: Q9P4Z1 (E3 ubiquitin-protein ligase TOM1-like OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=B11B22.010 PE=3 SV=4)

HSP 1 Score: 529.3 bits (1362), Expect = 3.6e-148
Identity = 671/2567 (26.14%), Postives = 1111/2567 (43.28%), Query Frame = 0

Query: 1365 IEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNR----SNVMSFLIE 1424
            +EE  +L   + L+   + ++ +     F V DL+     + D ++R      +++  + 
Sbjct: 1649 VEERAKL--YDTLIDRSLEVISSHPEAVFEVADLIQNTILKTDNEDRRVEVGEILANALM 1708

Query: 1425 SVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSF 1484
            S    D      N ++++A  H++AL+L      ++ A+    V +  + +  +   L  
Sbjct: 1709 SFASDDADELKENGSSIAAYAHLLALLL------QQTAFMRTTVDMLRDYVGDYLGFLKL 1768

Query: 1485 ---GVKVEVPKWVTAAFLAIDRLLQEEKKFNPEI--ADQLKRDNVGGDTLT-------ID 1544
                    +P W+    L  + +L ++ +  P+I     +K D+   +++        + 
Sbjct: 1769 PPASSNDSLPPWIPYILLIFEIMLFKDAQ-PPDIKWKQPVKEDDPIEESVIEVKEPNFLA 1828

Query: 1545 EDKQNKLQSALGLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVA 1604
            E + + L + L   P+   +G ++ L                  +VL++   LTR H+VA
Sbjct: 1829 EHRSSLLTTLLDFLPR---IGKEESLAV----------------SVLRILVVLTRDHAVA 1888

Query: 1605 VSFLEAGGLTSLLSLPTTSLFPGFDSIAST--------IIRHILEDPQTLQQAMESEIRH 1664
                E   L  L  +       G   +  T        I+RHI+ED +TL+Q  E++IRH
Sbjct: 1889 KIVGEKKNLQRLFVMAKQLCGAGSTRLKQTHISEHIRQILRHIIEDEETLRQIFETDIRH 1948

Query: 1665 TLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIE---------MIGERP 1724
             + +         + P+ +L     +  R P  F+  +  + ++           +   P
Sbjct: 1949 LMTSRQRPSAAPGLEPQAYLRTQAHLALRSPKTFVEVSTELLKLNRAVSHLGDGTLRSTP 2008

Query: 1725 YVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD---SR 1784
            ++V LK+R  D S      KE S+E       D+ +   +  P+T    +   HD   + 
Sbjct: 2009 FLV-LKERPADASVS---PKESSVEPAVQATEDLTI--SDVKPSTEVTDKD-MHDAPKNP 2068

Query: 1785 SSRVHKKSSQNFINVIELLLESVYTFIPPMKD-DVTVELTSGAQASSDMDIDVAAVKGKG 1844
            +  + +   ++   V+  LL  +  +    KD D    + +   A+S  + D  A     
Sbjct: 2069 AQDLKRPILEHPDGVVHFLLTELLNY----KDVDERENVPAPPAAASKPESDATAA---N 2128

Query: 1845 KAIASLSEDDANSQ--EASASLAKV-------------VFILKLLTEILLMY--ASSVHV 1904
            +A  S S D+ NS+  E    LAK               F+L  L E+L  Y  A    +
Sbjct: 2129 EATPSPSGDEQNSESKEKEKKLAKSPFKAEDHPIFIYRCFLLDCLAELLQSYNRAKVEFI 2188

Query: 1905 LIRRDTEVCCSRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRG 1964
              +R   V  + PV  R S       ++++IL+  +  S  S   + +    K   A   
Sbjct: 2189 NFKRSAPVQANAPVKPRSS-------VLNYILNDLLCFSPASGVPESIAMKKKAATAVPA 2248

Query: 1965 SQFLVASCVRSSEARRR--------------IFVE---VGSILNQFIDSCNGSRPPS--- 2024
               LVA   ++ E  +               +F+    + +IL  + D+     P     
Sbjct: 2249 RAVLVALVSKTGEKPQNRTHEPFEYDDEPDLLFIRRFVLDTILRAYKDASVSGEPADIRY 2308

Query: 2025 SDLQAFVDLLNDMLA------------ARTPTGSYITTEASATFVDAGLVRSFTQILKVL 2084
              + A  +L+  M+                P+      +      + G + S T  +  +
Sbjct: 2309 GKMNALAELMAQMIGEGDKDSRPNNPRGTDPSIGRSQAQLKRLMYEKGYLTSLTASIADI 2368

Query: 2085 DLDHPDSP---KVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKTPDHHQPGGENIGETP 2144
            DL  P +    K +  VIK L          G   A     + +  D     G ++ E  
Sbjct: 2369 DLTFPHNRAPLKPILAVIKTLSKTAVSMSQLGLIPASGTAGTDQAEDEFLSDGSSVSEDL 2428

Query: 2145 RAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTDDMEHDQDLHGVFGANAEDECMHVT 2204
                  +   + +     +EP   ++     E   +DM  D+          +DE  +  
Sbjct: 2429 TDDREETPDLYRNSTLGMLEPGRDDEFSDEDEDDDEDMYDDEQY--------DDELDYGD 2488

Query: 2205 PEEARGLENGIDTVDIQIEI--------QPHVPENLDEDDDEEMSGDDGDEVDEDEDEDE 2264
                   +N  D  D   E+        QP V E L  ++D+E   DD D++DED++ DE
Sbjct: 2489 DMSQDNEDNPSDEEDDLGEMGEMGGMPGQPGVVEVLMGENDDEEDNDDMDDMDEDDEMDE 2548

Query: 2265 DEDEEEQNDLEDDEVHHLPHPDTDHDDH-------EIDDD--EFDEVLEEDDEDDED-DE 2324
            D+++E  ++ ED+EV      D + D H        IDDD   +D+  +ED+ED+E+ D 
Sbjct: 2549 DDEQELSDEDEDEEVGSEDMDDLEDDIHIVDEEGNAIDDDGASWDDGTDEDEEDEEELDY 2608

Query: 2325 DGVLLRLEEG-INGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLGRT 2384
            +     ++E  ++    F  +     + +  + L   P+  F               G  
Sbjct: 2609 EAEAQNMQEAQLHNRRTFPEIMRAAMENA-GDDLDAEPIRDFDGHYIDDDED-----GEE 2668

Query: 2385 GDTVAPSRHPL-----LGGPGLH---ATPFRPSENNRDMVI--SERSLENNSSGLDTVFR 2444
             D        +       G GLH     P  PS    D+ I  + R   + S   ++ F 
Sbjct: 2669 DDDEDEGEDDMDDDMYFDGDGLHDDLLAPNMPSGLGWDIAIEPNHRHRPSRSPWPNSPF- 2728

Query: 2445 SLRSGRHGHRLNLWANDIQHNGGSNNGVIP-----QGLEELLVSQLRRPTPEKLSE--LD 2504
                GRH   ++ + N  +     +  + P      G   LL+ Q RR  P       + 
Sbjct: 2729 --VVGRHRDAID-FQNFFRRPAHLSRHLPPPADVMSGTNPLLLPQSRREVPRHAHSQLVR 2788

Query: 2505 ANAEPDNRDGNGQIQTSEPSGRSETIVEN-------------------SGQHGNDN---- 2564
                P+   G G     EP      +V++                    G  G       
Sbjct: 2789 LGITPNMIGGLGMGMGVEPLAFISDLVQHLPDVRLSAGGGPLALHFTADGPGGIIRELNA 2848

Query: 2565 --LPPPVAINSSDGTNSRPAASESLQGTQVIQQS------QPVEM--QFEHNDAA----- 2624
              +PPP +  S      R    E  Q  Q   +S      Q V+M   F + D A     
Sbjct: 2849 IPIPPPHSRESRPTEARRDTYQEPHQAVQFSPESTHERWQQEVKMIFGFGYQDKAQKLAP 2908

Query: 2625 ---VRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHD----DGGDRQGSADRMSLGDS 2684
                +   A  Q      A   E+ R  + E       +    +  + +  A R      
Sbjct: 2909 LILSKLTPAAIQAEKEEKARKAEADRKAEEERKKRQEEERKKREAKEAEEKAAREKKEAE 2968

Query: 2685 QAARMRRSNV-SFTNSASLSGRDTSLHIVTEVS------------ENSSREADEEGP--- 2744
            +  R+ R    +   +A+ +  D   + V++ +            EN+ ++A++E P   
Sbjct: 2969 ERERLERERAEAAAQAAAQAAADQEANAVSQEAHPMEGVETQGPGENAEQQAEDERPRVY 3028

Query: 2745 -VGEQQTNSDTGLGAIDPAFLDALPEELRAEV----LSTQQGQVVQPPSNEPQNSGDIDP 2804
                 Q    T LG ID  +L+ALPEE R EV    +ST++ Q  +  S E +N+ ++  
Sbjct: 3029 YTLRNQQIDITELG-IDAEYLEALPEEFRDEVIAQAISTRRSQAREQVSQEGENT-EVFQ 3088

Query: 2805 EFLAALPPDIRAEVLAQQQ-AQRLHLSQELEG----QPVEMDTVSIIATFPSDLREEVLL 2864
            EFL ALP ++R E+L Q+Q  QR    Q   G     P +MD  SI+ TFP  LR++VLL
Sbjct: 3089 EFLEALPEELRNEILHQEQHEQRRRERQNAAGGQDLGPADMDPASILLTFPPGLRQQVLL 3148

Query: 2865 TSSDAILANLTPALVAEANMLRERFAHRYHNRSLFGMHPRNRRGEITRRVEGISGLDRTG 2924
               + I+ +L P L AEA  L  R    +  +   G    +R  +  R  E  +G   T 
Sbjct: 3149 DQGEDIMEHLGPELAAEARTLVARHRQLHAQQ---GGQAASRSRDAQRPTEAGAG---TV 3208

Query: 2925 GSISSRRSLGSRLIEAEGAPLVDTDALHAMIRLLRVVQP-LYKGQLQRLLLNLCAHNETR 2984
              I  R  +           ++D   +  ++RL+ V Q    +  L  +  NLC + + R
Sbjct: 3209 QKIQKRTVV----------QMLDKQGIATLLRLMFVSQQGSIRSSLFSIFANLCENRQNR 3268

Query: 2985 TSLVKILMDVLLFD-RRKPANQSNTTELSYRL-----------YACQRNVIYSR------ 3044
              ++  L+ +L        A + +  ++S++            +  +R++          
Sbjct: 3269 LDVISSLLQILQDGCANMDAVERSFAQISHKAKQLKEKDAKTPHPLKRSLTGGTNNNGQF 3328

Query: 3045 PSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAV 3104
            P+  + +P L+ ++ L+ L  L++ +PH+  +          L E E V     +++   
Sbjct: 3329 PASSEVSPLLIVQQCLDLLVELSKLNPHIPSVF---------LTEHETVASTLKRSLSRK 3388

Query: 3105 DR-NEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLL---------------- 3164
             +  +++ + Q     +I  LL+LL++ L + S A ++ L +LL                
Sbjct: 3389 GKGKDVNGKAQK---FAINSLLTLLDRSLVMESSAVMQVLADLLNKVTIPLQAIERRRKE 3448

Query: 3165 ------------EVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGV 3224
                        E      E+ +   +Q + ST + PA  E +   +E    ++S     
Sbjct: 3449 AEEQAKKKKEAEEKAATEREAANAPEEQASTSTEQTPAQQEATQQPSESTPAAASGQQPA 3508

Query: 3225 --GTSSKLGSYFKMAASAADGEFDIHSIL----ANLPEAELRLLCSLLAREGLSDNTYAL 3284
                 +K     K  A   D + D   I       +PE  L+L+ ++      S  T+  
Sbjct: 3509 QQDQENKELEAPKEKADEKDVQSDEKKIRQLTPPTIPEHNLKLVINIFVARECSSKTFQN 3568

Query: 3285 VGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAMDEL-HMFGEAVKALSISSSDGAAIL 3344
                +K L +I P   ++F  EL      L+ + + +L ++    +KA S +   G A+ 
Sbjct: 3569 TISTIKNLSNI-PGAKKVFGDELVRQARVLSENILSDLDNLLPHILKAESGTQIQGVALA 3628

Query: 3345 ----------RVLQALSSLVALLVEKRKDSSILPEKEHASA-LTLVCDINAALEPLWLEL 3404
                      ++L+ L++L  L   K K      E E+    L      N     +W +L
Sbjct: 3629 KFSPGASEQNKLLRVLTALDHLFDSKSKKQDKPAEGENTKEDLLGSLYWNPTFGKMWDKL 3688

Query: 3405 STCIGKIESYSDSSPDTLVSFRTPTSKPTGVTPPLPAGSQNILPYIESFFVVCEKLHPAQ 3464
            S C+  I        D +++  T                  +LP IES  VVC       
Sbjct: 3689 SACLSAIR-----QRDNMLNVAT-----------------ILLPLIESLMVVC------- 3748

Query: 3465 PGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNP 3524
                +   ++  S V +A +   +  T+ P +  D     F  F+E+HR++LN  +R NP
Sbjct: 3749 ----KNTTLSDASAVSNANSQKEMLLTSPPPE--DRIAGLFFTFTEEHRRILNELVRHNP 3808

Query: 3525 GLLEKSFSPMLKVPRFIDFDNKRSHFRSRI--KHQHDHHS--PLRISVRRAYILEDSYNQ 3584
             L+  +FS ++K P+ ++FDNKR++F   +  K+Q   HS  PL++ VRR ++  DS+  
Sbjct: 3809 KLMSGTFSLLVKNPKVLEFDNKRNYFNRSVHSKYQQTRHSFPPLQLQVRREHVFHDSFRS 3868

Query: 3585 LRMRSTQDLK-GRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGALLFTTVGND-STFQ 3644
            L  +   +LK G+L + FQGEEG+DAGG++REW+QVLSR +FD   +LF  V +D +TF 
Sbjct: 3869 LYYKKADELKFGKLNIRFQGEEGVDAGGVTREWFQVLSRQMFDPNYVLFVPVSSDRTTFH 3928

Query: 3645 PNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTYHDIEAIDPD 3664
            PN  S    EHL +FKF+GR++GKALY+G+LL+ +F+R+ YK ILG  V+  D+E+ DPD
Sbjct: 3929 PNKLSPINDEHLPFFKFIGRIIGKALYEGRLLECYFSRAVYKRILGKPVSVKDMESFDPD 3988

BLAST of CmoCh12G001650 vs. ExPASy TrEMBL
Match: A0A6J1GH79 (HECT-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111454182 PE=4 SV=1)

HSP 1 Score: 7085.0 bits (18381), Expect = 0.0e+00
Identity = 3664/3664 (100.00%), Postives = 3664/3664 (100.00%), Query Frame = 0

Query: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60
            MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW
Sbjct: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60

Query: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLE 120
            EYNKGNYHHWRPLFLHFDTYFKTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLE
Sbjct: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLE 120

Query: 121  NCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL 180
            NCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL
Sbjct: 121  NCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL 180

Query: 181  LSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC 240
            LSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC
Sbjct: 181  LSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC 240

Query: 241  GAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA 300
            GAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA
Sbjct: 241  GAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA 300

Query: 301  RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT 360
            RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT
Sbjct: 301  RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT 360

Query: 361  IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA 420
            IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA
Sbjct: 361  IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA 420

Query: 421  FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF 480
            FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF
Sbjct: 421  FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF 480

Query: 481  SSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK 540
            SSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK
Sbjct: 481  SSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK 540

Query: 541  VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL 600
            VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL
Sbjct: 541  VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL 600

Query: 601  HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE 660
            HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE
Sbjct: 601  HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE 660

Query: 661  KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGN 720
            KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGN
Sbjct: 661  KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGN 720

Query: 721  TAMETDSDDKDNNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 780
            TAMETDSDDKDNNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL
Sbjct: 721  TAMETDSDDKDNNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 780

Query: 781  LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG 840
            LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG
Sbjct: 781  LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG 840

Query: 841  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ 900
            SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ
Sbjct: 841  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ 900

Query: 901  ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS 960
            ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS
Sbjct: 901  ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS 960

Query: 961  VESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQRRSESSDTIGASNRKEYSKH 1020
            VESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQRRSESSDTIGASNRKEYSKH
Sbjct: 961  VESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQRRSESSDTIGASNRKEYSKH 1020

Query: 1021 RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLSSLVLDH 1080
            RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLSSLVLDH
Sbjct: 1021 RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLSSLVLDH 1080

Query: 1081 MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT 1140
            MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT
Sbjct: 1081 MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT 1140

Query: 1141 TFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHLVTSPFI 1200
            TFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHLVTSPFI
Sbjct: 1141 TFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHLVTSPFI 1200

Query: 1201 LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI 1260
            LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI
Sbjct: 1201 LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI 1260

Query: 1261 SIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVGSNSVEL 1320
            SIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVGSNSVEL
Sbjct: 1261 SIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVGSNSVEL 1320

Query: 1321 AMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCTEELLST 1380
            AMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCTEELLST
Sbjct: 1321 AMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCTEELLST 1380

Query: 1381 CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNSTTLSAL 1440
            CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNSTTLSAL
Sbjct: 1381 CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNSTTLSAL 1440

Query: 1441 FHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL 1500
            FHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL
Sbjct: 1441 FHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL 1500

Query: 1501 LQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS 1560
            LQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS
Sbjct: 1501 LQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS 1560

Query: 1561 CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII 1620
            CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII
Sbjct: 1561 CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII 1620

Query: 1621 RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQS 1680
            RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQS
Sbjct: 1621 RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQS 1680

Query: 1681 VCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNV 1740
            VCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNV
Sbjct: 1681 VCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNV 1740

Query: 1741 RSKFHDSRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV 1800
            RSKFHDSRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV
Sbjct: 1741 RSKFHDSRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV 1800

Query: 1801 AAVKGKGKAIASLSEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC 1860
            AAVKGKGKAIASLSEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC
Sbjct: 1801 AAVKGKGKAIASLSEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC 1860

Query: 1861 SRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR 1920
            SRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR
Sbjct: 1861 SRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR 1920

Query: 1921 SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTGSYITTEASA 1980
            SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTGSYITTEASA
Sbjct: 1921 SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTGSYITTEASA 1980

Query: 1981 TFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT 2040
            TFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT
Sbjct: 1981 TFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT 2040

Query: 2041 PDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTDDMEHDQDLH 2100
            PDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTDDMEHDQDLH
Sbjct: 2041 PDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTDDMEHDQDLH 2100

Query: 2101 GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEVD 2160
            GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEVD
Sbjct: 2101 GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEVD 2160

Query: 2161 EDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDEDDEDGV 2220
            EDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDEDDEDGV
Sbjct: 2161 EDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDEDDEDGV 2220

Query: 2221 LLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLGRTGDTV 2280
            LLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLGRTGDTV
Sbjct: 2221 LLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLGRTGDTV 2280

Query: 2281 APSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHGHRLNLW 2340
            APSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHGHRLNLW
Sbjct: 2281 APSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHGHRLNLW 2340

Query: 2341 ANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQTSEPSG 2400
            ANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQTSEPSG
Sbjct: 2341 ANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQTSEPSG 2400

Query: 2401 RSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQFEHNDA 2460
            RSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQFEHNDA
Sbjct: 2401 RSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQFEHNDA 2460

Query: 2461 AVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMR 2520
            AVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMR
Sbjct: 2461 AVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMR 2520

Query: 2521 RSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDA 2580
            RSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDA
Sbjct: 2521 RSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDA 2580

Query: 2581 LPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQ 2640
            LPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQ
Sbjct: 2581 LPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQ 2640

Query: 2641 ELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR 2700
            ELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR
Sbjct: 2641 ELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR 2700

Query: 2701 SLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLVDTDALHAMIR 2760
            SLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLVDTDALHAMIR
Sbjct: 2701 SLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLVDTDALHAMIR 2760

Query: 2761 LLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELSYRLYA 2820
            LLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELSYRLYA
Sbjct: 2761 LLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELSYRLYA 2820

Query: 2821 CQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQ 2880
            CQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQ
Sbjct: 2821 CQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQ 2880

Query: 2881 DCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAE 2940
            DCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAE
Sbjct: 2881 DCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAE 2940

Query: 2941 SKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGE 3000
            SKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGE
Sbjct: 2941 SKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGE 3000

Query: 3001 FDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSES 3060
            FDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSES
Sbjct: 3001 FDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSES 3060

Query: 3061 VEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKE 3120
            VEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKE
Sbjct: 3061 VEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKE 3120

Query: 3121 HASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTPPLPAG 3180
            HASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTPPLPAG
Sbjct: 3121 HASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTPPLPAG 3180

Query: 3181 SQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKH 3240
            SQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKH
Sbjct: 3181 SQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKH 3240

Query: 3241 IAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHHS 3300
            IAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHHS
Sbjct: 3241 IAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHHS 3300

Query: 3301 PLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFD 3360
            PLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFD
Sbjct: 3301 PLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFD 3360

Query: 3361 KGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHI 3420
            KGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHI
Sbjct: 3361 KGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHI 3420

Query: 3421 LGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELI 3480
            LGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELI
Sbjct: 3421 LGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELI 3480

Query: 3481 PGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFNDKELELL 3540
            PGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFNDKELELL
Sbjct: 3481 PGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFNDKELELL 3540

Query: 3541 ICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTSKVPLEG 3600
            ICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTSKVPLEG
Sbjct: 3541 ICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTSKVPLEG 3600

Query: 3601 FSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAIHEANEG 3660
            FSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAIHEANEG
Sbjct: 3601 FSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAIHEANEG 3660

Query: 3661 FGFG 3665
            FGFG
Sbjct: 3661 FGFG 3664

BLAST of CmoCh12G001650 vs. ExPASy TrEMBL
Match: A0A6J1KNT1 (HECT-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495915 PE=4 SV=1)

HSP 1 Score: 6964.4 bits (18068), Expect = 0.0e+00
Identity = 3609/3670 (98.34%), Postives = 3632/3670 (98.96%), Query Frame = 0

Query: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60
            MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW
Sbjct: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60

Query: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKDLLSDKILEDDSPFPKHAILQILRVMQIVLE 120
            EYNKGNYHHWRPLFLHFDTYFKTYLSCR DLLSDKILEDDSPFPKHAILQILRVMQIVLE
Sbjct: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRNDLLSDKILEDDSPFPKHAILQILRVMQIVLE 120

Query: 121  NCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL 180
            NCHNKGSLDGLEYF LLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL
Sbjct: 121  NCHNKGSLDGLEYFKLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSYL 180

Query: 181  LSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC 240
            LSLAQGWGSKEEGLGLYSCVVA+EITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC
Sbjct: 181  LSLAQGWGSKEEGLGLYSCVVANEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHGC 240

Query: 241  GAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA 300
            GAKSSDESSSSSSSSNS VIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA
Sbjct: 241  GAKSSDESSSSSSSSNSSVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRYA 300

Query: 301  RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT 360
            RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT
Sbjct: 301  RAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPGT 360

Query: 361  IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA 420
            IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA
Sbjct: 361  IRTLAMRALGAQFSAYSSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSSLA 420

Query: 421  FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF 480
            FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF
Sbjct: 421  FVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDF 480

Query: 481  SSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK 540
            SSSAVSLFKELGGVE+LVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK
Sbjct: 481  SSSAVSLFKELGGVELLVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIK 540

Query: 541  VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL 600
            VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL
Sbjct: 541  VSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLL 600

Query: 601  HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE 660
            HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE
Sbjct: 601  HELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKE 660

Query: 661  KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGN 720
            KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPV SSGKLNGN
Sbjct: 661  KYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVGSSGKLNGN 720

Query: 721  TAMETDSDDKDNNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 780
            TAMETDSDDK+NNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL
Sbjct: 721  TAMETDSDDKENNSIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 780

Query: 781  LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG 840
            LKLLLRP+IAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTG DLVSG
Sbjct: 781  LKLLLRPSIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGIDLVSG 840

Query: 841  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ 900
            SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWV ALLTEFGNESKNVLEDIGRVHRQ
Sbjct: 841  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVTALLTEFGNESKNVLEDIGRVHRQ 900

Query: 901  ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS 960
            ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS
Sbjct: 901  ILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRRTSGWS 960

Query: 961  VESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQRRSESSDTIGASNRKEYSKH 1020
            VESQFFDLINLYRDLGRAPSSLQRMG NGSP LQ GANNQ RSE SDTIGASN KEYSKH
Sbjct: 961  VESQFFDLINLYRDLGRAPSSLQRMGSNGSPPLQLGANNQGRSE-SDTIGASNGKEYSKH 1020

Query: 1021 RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLSSLVLDH 1080
            RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSS+KAVASTLSSLVLDH
Sbjct: 1021 RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSAKAVASTLSSLVLDH 1080

Query: 1081 MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT 1140
            MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT
Sbjct: 1081 MNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGVVSSVLT 1140

Query: 1141 TFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHLVTSPFI 1200
            TFEATSQLLFTINRAPSSPMETDDANLKQDEK DNDHSWIQGPLASYGRLMDHLVTSPFI
Sbjct: 1141 TFEATSQLLFTINRAPSSPMETDDANLKQDEKADNDHSWIQGPLASYGRLMDHLVTSPFI 1200

Query: 1201 LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI 1260
            LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI
Sbjct: 1201 LSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCEFITTVI 1260

Query: 1261 SIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVGSNSVEL 1320
            SIIRHIYSGVEVKSVSSNSSARIA PPPNETAISTIVEMGFSR RAEEALRQVGSNSVEL
Sbjct: 1261 SIIRHIYSGVEVKSVSSNSSARIAVPPPNETAISTIVEMGFSRSRAEEALRQVGSNSVEL 1320

Query: 1321 AMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCTEELLST 1380
            AMEWLFSHPEEV+EDDELARALALSLGNSELEMKEPVSSEVS QIEESVQLPCTEELLST
Sbjct: 1321 AMEWLFSHPEEVREDDELARALALSLGNSELEMKEPVSSEVSNQIEESVQLPCTEELLST 1380

Query: 1381 CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNSTTLSAL 1440
            CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLI+SVKGCDKISDSGNSTTLSAL
Sbjct: 1381 CVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIDSVKGCDKISDSGNSTTLSAL 1440

Query: 1441 FHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL 1500
            FHVIALILNDDTVAREAAY+NGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL
Sbjct: 1441 FHVIALILNDDTVAREAAYKNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAFLAIDRL 1500

Query: 1501 LQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS 1560
            LQEEKKFNPEIADQLKRDNVGGD LTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS
Sbjct: 1501 LQEEKKFNPEIADQLKRDNVGGDALTIDEDKQNKLQSALGLSPKYIDVGNQKELIEIACS 1560

Query: 1561 CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII 1620
            CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII
Sbjct: 1561 CIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTII 1620

Query: 1621 RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQS 1680
            RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRA QS
Sbjct: 1621 RHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRAAQS 1680

Query: 1681 VCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNV 1740
            VCQIEMIGERPYV LLKDREKDKSKEKDR+KEKSMEKEKTQNHDVKVPLGNANPATAGNV
Sbjct: 1681 VCQIEMIGERPYVALLKDREKDKSKEKDREKEKSMEKEKTQNHDVKVPLGNANPATAGNV 1740

Query: 1741 RSKFHDSRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV 1800
            RSK HDS+SSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV
Sbjct: 1741 RSKLHDSKSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDV 1800

Query: 1801 AAVKGKGKAIASLSEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC 1860
            AAVKGKGKAIASLSEDDANS EASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC
Sbjct: 1801 AAVKGKGKAIASLSEDDANSLEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCC 1860

Query: 1861 SRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR 1920
            SRPVHQRES+GGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR
Sbjct: 1861 SRPVHQRESSGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGSQFLVASCVR 1920

Query: 1921 SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTGSYITTEASA 1980
            SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLND L ARTPTGSYITTEASA
Sbjct: 1921 SSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDTLTARTPTGSYITTEASA 1980

Query: 1981 TFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT 2040
            TFVDA LVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT
Sbjct: 1981 TFVDACLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNAGKGDSSSKT 2040

Query: 2041 PDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTDDMEHDQDLH 2100
            PDH+QPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVT+DMEHDQDLH
Sbjct: 2041 PDHNQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTEDMEHDQDLH 2100

Query: 2101 GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEV- 2160
            GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEV 
Sbjct: 2101 GVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDEVD 2160

Query: 2161 -----DEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDE 2220
                 DEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDE
Sbjct: 2161 EDEDEDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDEDDE 2220

Query: 2221 DDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLG 2280
            DDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLG
Sbjct: 2221 DDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYNLLG 2280

Query: 2281 RTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHG 2340
            RTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHG
Sbjct: 2281 RTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSGRHG 2340

Query: 2341 HRLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQ 2400
            HRLNLWANDIQ NGGSN+GVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQ
Sbjct: 2341 HRLNLWANDIQQNGGSNSGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQ 2400

Query: 2401 TSEPSGRSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQ 2460
            TSEPSGRSETIVENSGQH +DNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQ
Sbjct: 2401 TSEPSGRSETIVENSGQHDSDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPVEMQ 2460

Query: 2461 FEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDS 2520
            FEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDS
Sbjct: 2461 FEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDS 2520

Query: 2521 QAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLGAID 2580
            QAARMRRSN+SFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTG GAID
Sbjct: 2521 QAARMRRSNISFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGSGAID 2580

Query: 2581 PAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQ 2640
            PAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQ
Sbjct: 2581 PAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQ 2640

Query: 2641 RLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFA 2700
            RLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFA
Sbjct: 2641 RLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLRERFA 2700

Query: 2701 HRYHNRSLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLVDTDA 2760
            HRYHNR+LFGMHPR+RRGEITRRVEGISGLDRTGGS SSRRSLGSRLIEAEGAPLVDTDA
Sbjct: 2701 HRYHNRTLFGMHPRHRRGEITRRVEGISGLDRTGGSTSSRRSLGSRLIEAEGAPLVDTDA 2760

Query: 2761 LHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTEL 2820
            LHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRK ANQSN+TEL
Sbjct: 2761 LHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKLANQSNSTEL 2820

Query: 2821 SYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQE 2880
            SYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQE
Sbjct: 2821 SYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQE 2880

Query: 2881 SENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEV 2940
            SENVNQDCGKAVMAVDRNE+DCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEV
Sbjct: 2881 SENVNQDCGKAVMAVDRNEVDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEV 2940

Query: 2941 IIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAA 3000
            IIDNAESKSHLS+QPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLG+YFKMAA
Sbjct: 2941 IIDNAESKSHLSNQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGAYFKMAA 3000

Query: 3001 SAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFI 3060
            SAADGEFDIHSIL NLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFI
Sbjct: 3001 SAADGEFDIHSILENLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFI 3060

Query: 3061 TELSESVEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSS 3120
            TELSESVEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSS
Sbjct: 3061 TELSESVEKLTRSAMDELHMFGEAVKALSISSSDGAAILRVLQALSSLVALLVEKRKDSS 3120

Query: 3121 ILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVT 3180
            ILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPD LVSFRTPTSKPTGVT
Sbjct: 3121 ILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDMLVSFRTPTSKPTGVT 3180

Query: 3181 PPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQ 3240
            PPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQ
Sbjct: 3181 PPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQ 3240

Query: 3241 KVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKH 3300
            KVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKH
Sbjct: 3241 KVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKH 3300

Query: 3301 QHDHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVL 3360
            QHDHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVL
Sbjct: 3301 QHDHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVL 3360

Query: 3361 SRVIFDKGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTR 3420
            SRVIFDKGALLFTTVGND+TFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTR
Sbjct: 3361 SRVIFDKGALLFTTVGNDATFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTR 3420

Query: 3421 SFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEV 3480
            SFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEV
Sbjct: 3421 SFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEV 3480

Query: 3481 TDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFND 3540
            TDYELIPGGRN KVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFND
Sbjct: 3481 TDYELIPGGRNIKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFND 3540

Query: 3541 KELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTS 3600
            KELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTS
Sbjct: 3541 KELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTS 3600

Query: 3601 KVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAI 3660
            KVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAI
Sbjct: 3601 KVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAI 3660

Query: 3661 HEANEGFGFG 3665
            HEANEGFGFG
Sbjct: 3661 HEANEGFGFG 3669

BLAST of CmoCh12G001650 vs. ExPASy TrEMBL
Match: A0A6J1BQP3 (HECT-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111004867 PE=4 SV=1)

HSP 1 Score: 6415.9 bits (16644), Expect = 0.0e+00
Identity = 3338/3678 (90.76%), Postives = 3480/3678 (94.62%), Query Frame = 0

Query: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60
            MT QRSSLPSRLRQLLSGEGSFGPS+KLDSEPPPKIK FIDKVIQCPLHDIAI LSGFRW
Sbjct: 1    MTAQRSSLPSRLRQLLSGEGSFGPSIKLDSEPPPKIKDFIDKVIQCPLHDIAIPLSGFRW 60

Query: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKDL-LSDKILEDDSPFPKHAILQILRVMQIVL 120
            EYNKGNYHHWRPLFLHFDTYFKTYLSCR DL LSDKILEDDSPFPKHAILQILRVMQIVL
Sbjct: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRNDLILSDKILEDDSPFPKHAILQILRVMQIVL 120

Query: 121  ENCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180
            ENCHNKGSLDGLE+F LLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY
Sbjct: 121  ENCHNKGSLDGLEHFKLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180

Query: 181  LLSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHG 240
            LLSLAQGWGSKEEGLGLYSCVVA+E T+EEG CLFP DVEND ++AQYRIGSSLYFELH 
Sbjct: 181  LLSLAQGWGSKEEGLGLYSCVVANERTQEEGFCLFPADVENDIDSAQYRIGSSLYFELHC 240

Query: 241  CGAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRY 300
            CGAK+++ESSSSSSSSNS+VIHIPDLHLKKEDDL VLKQCIELY+VPPELRFSLLTRIRY
Sbjct: 241  CGAKNTEESSSSSSSSNSKVIHIPDLHLKKEDDLMVLKQCIELYNVPPELRFSLLTRIRY 300

Query: 301  ARAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPG 360
            ARAF SS + RLYS+ICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETV G
Sbjct: 301  ARAFRSSKICRLYSRICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVSG 360

Query: 361  TIRTLAMRALGAQFSAY-SSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSS 420
             IRTLAM ALGAQ +AY SSHERRILSGSSISFAGGNR ILLNVLQKA+LSLKNSNDPSS
Sbjct: 361  AIRTLAMLALGAQLAAYSSSHERRILSGSSISFAGGNRMILLNVLQKAVLSLKNSNDPSS 420

Query: 421  LAFVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLM 480
            LAFVEALLQFYLLH+VSSSASGNNIR SGMVPTFLPLLEDSDPSHLHLVC AVKTLQKLM
Sbjct: 421  LAFVEALLQFYLLHVVSSSASGNNIRGSGMVPTFLPLLEDSDPSHLHLVCFAVKTLQKLM 480

Query: 481  DFSSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRM 540
            DFSSSAVSLFKELGGVE+LVERLQTEV RVIG+SG N+DSMIIGESS CNDDQLYNQKR+
Sbjct: 481  DFSSSAVSLFKELGGVEVLVERLQTEVQRVIGVSGTNMDSMIIGESSSCNDDQLYNQKRL 540

Query: 541  IKVSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYP 600
            IKVSLKALGVATYVP++STN+LP ILS IFGN+DKFGGDIYCSAVTLMSEIIHKDPTCYP
Sbjct: 541  IKVSLKALGVATYVPSNSTNSLPAILSQIFGNIDKFGGDIYCSAVTLMSEIIHKDPTCYP 600

Query: 601  LLHELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFT 660
             LHE+GLPDAFLASVAAGILPS KA+TCVPNGIGAICLNARGLEAVKET ALRFL+DIFT
Sbjct: 601  SLHEMGLPDAFLASVAAGILPSSKAVTCVPNGIGAICLNARGLEAVKETSALRFLVDIFT 660

Query: 661  KEKYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLN 720
            KEKYVL MNEAIVPLANAVEEL RHVSSLRS+GVDIILEVIEKI SLG+ DP+ SSGKLN
Sbjct: 661  KEKYVLAMNEAIVPLANAVEELLRHVSSLRSTGVDIILEVIEKIASLGENDPIVSSGKLN 720

Query: 721  GNTAMETDSDDKDNNS---IVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENS 780
            GN AMETDSDDK+NNS   +VTEE     Q +QLCIFHLMVLVHRTMENSETCRIFVENS
Sbjct: 721  GNAAMETDSDDKENNSNCALVTEE----GQFIQLCIFHLMVLVHRTMENSETCRIFVENS 780

Query: 781  GIEALLKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGF 840
            GIEALLKLLLRP+IAQS+NGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKK LTGF
Sbjct: 781  GIEALLKLLLRPSIAQSSNGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKALTGF 840

Query: 841  DLVSGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIG 900
             LVSGSFLLDPRTTPDEKIF+SLFLVEFLLFLADSKDNRWV ALLTEFGNESK+VLEDIG
Sbjct: 841  GLVSGSFLLDPRTTPDEKIFTSLFLVEFLLFLADSKDNRWVTALLTEFGNESKDVLEDIG 900

Query: 901  RVHRQILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRR 960
            RVHR+ILWQIALLEDVKPE ED+ T SITE QNSEVHTNEIEEQRFNSFRQFLDPLLRRR
Sbjct: 901  RVHREILWQIALLEDVKPESEDDGTRSITESQNSEVHTNEIEEQRFNSFRQFLDPLLRRR 960

Query: 961  TSGWSVESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQ-RRSESSDTIGASNR 1020
            TSGWS+ESQFFDLINLYRDLGRAPSSLQRMG +GSPLLQFGA NQ  RS SSDT GASN 
Sbjct: 961  TSGWSIESQFFDLINLYRDLGRAPSSLQRMGSDGSPLLQFGAGNQGPRSGSSDTTGASNG 1020

Query: 1021 KEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080
            KEYSK RTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS
Sbjct: 1021 KEYSKQRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080

Query: 1081 SLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGV 1140
            SLV DHMNF G VNASGSEGSISTKCRY+GKVIDFVDGILLDRPDSCN VLLNCLYGHGV
Sbjct: 1081 SLVSDHMNFGGRVNASGSEGSISTKCRYYGKVIDFVDGILLDRPDSCNPVLLNCLYGHGV 1140

Query: 1141 VSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHL 1200
            V  VLTTF+ATSQLLFT+NRAP+SPMETDDANLKQDEK DNDHSWIQGPLASYGRLMDHL
Sbjct: 1141 VKLVLTTFDATSQLLFTLNRAPASPMETDDANLKQDEKADNDHSWIQGPLASYGRLMDHL 1200

Query: 1201 VTSPFILSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCE 1260
            VTSPFILSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFI+CSCE
Sbjct: 1201 VTSPFILSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIECSCE 1260

Query: 1261 FITTVISIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVG 1320
            FITTVISI+RHIYSGVEVK+VSSNSSARI GPPPNETAISTIVEMGFSR RAEEALRQVG
Sbjct: 1261 FITTVISIMRHIYSGVEVKNVSSNSSARITGPPPNETAISTIVEMGFSRSRAEEALRQVG 1320

Query: 1321 SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCT 1380
            SNSVELAMEWLFSHPE+VQEDDELARALALSLG+SELEMKEPVSSEVSKQIEE+V LPCT
Sbjct: 1321 SNSVELAMEWLFSHPEDVQEDDELARALALSLGSSELEMKEPVSSEVSKQIEEAVHLPCT 1380

Query: 1381 EELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNS 1440
            EELLSTC++LLRAKETLAFPVRDLLVMICSQNDGQNRSNV+SFLI+SVKGCD I+DSGNS
Sbjct: 1381 EELLSTCIKLLRAKETLAFPVRDLLVMICSQNDGQNRSNVISFLIDSVKGCDTIADSGNS 1440

Query: 1441 TTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAF 1500
             TLSALFHVIALILNDDTVARE  Y+NGLV V+SNLLSQWDSGL+  VKVEVPKWVTAAF
Sbjct: 1441 ITLSALFHVIALILNDDTVARETVYKNGLVAVASNLLSQWDSGLADDVKVEVPKWVTAAF 1500

Query: 1501 LAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKEL 1560
            LAIDRLLQEEKK NPEIADQLKRDNVGGD+LTIDEDKQNKLQSAL  SPKYIDVGNQK+L
Sbjct: 1501 LAIDRLLQEEKKLNPEIADQLKRDNVGGDSLTIDEDKQNKLQSALVFSPKYIDVGNQKKL 1560

Query: 1561 IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDS 1620
            IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFL+AGGLTSLLSLPT+SLFPGFDS
Sbjct: 1561 IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLDAGGLTSLLSLPTSSLFPGFDS 1620

Query: 1621 IASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIF 1680
            IASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVI+RDP+IF
Sbjct: 1621 IASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVITRDPMIF 1680

Query: 1681 MRATQSVCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANP 1740
            MRA QSVCQIEM+GERPY+VLLKDREK+K KEKDR+KEKSMEKEK+QNHDVKV LGN NP
Sbjct: 1681 MRAAQSVCQIEMVGERPYIVLLKDREKEKGKEKDREKEKSMEKEKSQNHDVKVALGNVNP 1740

Query: 1741 ATAGNVRSKFHD--SRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQA 1800
               GNV  K HD  S+SSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVT+EL+  A+A
Sbjct: 1741 VVVGNVHGKLHDSNSKSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTMELSCNARA 1800

Query: 1801 SSDMDIDVAAVKGKGKAIASLSED-DANSQEASASLAKVVFILKLLTEILLMYASSVHVL 1860
            SSDMDIDVAAVKGKGKA+ASLSED DA+SQEASASLAKVVFILKLLTEILLMY SSVHVL
Sbjct: 1801 SSDMDIDVAAVKGKGKAVASLSEDNDASSQEASASLAKVVFILKLLTEILLMYGSSVHVL 1860

Query: 1861 IRRDTEVCCSRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGS 1920
            +R+DTEVCCS+PVHQR S+  C GGI HHILH FIPLSRNSKKEKKVDGDWKHKLATRGS
Sbjct: 1861 LRKDTEVCCSKPVHQRGSSVVCIGGIFHHILHEFIPLSRNSKKEKKVDGDWKHKLATRGS 1920

Query: 1921 QFLVASCVRSSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTG 1980
            QFLVASCVRSSEARRRIF+E+GSILNQFIDSCNG RPPSSDLQAFVDL+NDMLAARTPTG
Sbjct: 1921 QFLVASCVRSSEARRRIFMEIGSILNQFIDSCNGFRPPSSDLQAFVDLVNDMLAARTPTG 1980

Query: 1981 SYITTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNA 2040
            SYITTEASATF+DAGLVRSFTQILKV+DLDH DSPKVVTG++KALELVTKEHV F DSN 
Sbjct: 1981 SYITTEASATFIDAGLVRSFTQILKVMDLDHTDSPKVVTGLVKALELVTKEHVQFADSNT 2040

Query: 2041 GKGDSSSKTPDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTD 2100
            GKG+SSSKTPDH+QPG +NIGETPR++E  SQSNHES+PA+QIEPYN NQ YGGSEAVTD
Sbjct: 2041 GKGNSSSKTPDHNQPGVQNIGETPRSLEIASQSNHESMPADQIEPYNINQNYGGSEAVTD 2100

Query: 2101 DMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEM 2160
            DMEHDQDL GVFG NAEDE MH TPE+A GLENGIDTVDI+IEIQPHVPENLDEDDDEEM
Sbjct: 2101 DMEHDQDLDGVFGPNAEDEYMHETPEDAGGLENGIDTVDIRIEIQPHVPENLDEDDDEEM 2160

Query: 2161 SGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE 2220
            SGDDGDEV  D+D+DEDE+EEEQNDLE+D+VHHLPHPDTDHDDHE+DDDEFDEVLEEDDE
Sbjct: 2161 SGDDGDEV--DDDDDEDEEEEEQNDLEEDDVHHLPHPDTDHDDHEMDDDEFDEVLEEDDE 2220

Query: 2221 DDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280
            +DEDDEDGV+LRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN
Sbjct: 2221 EDEDDEDGVILRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280

Query: 2281 LLGRTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSG 2340
            LLGRTGD VAPSRHPLLGGP LH  PFRPSENNRDMVISERSLENNSSGLD VFRSLRSG
Sbjct: 2281 LLGRTGDNVAPSRHPLLGGPALHQAPFRPSENNRDMVISERSLENNSSGLDNVFRSLRSG 2340

Query: 2341 RHGHRLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNG 2400
            RHGHRLNLWAND QH+GGS+NGVIPQGLEELLVSQLRRPTPEK SE     EP+++DG G
Sbjct: 2341 RHGHRLNLWANDNQHSGGSSNGVIPQGLEELLVSQLRRPTPEKPSEHSVTIEPESKDGIG 2400

Query: 2401 QIQTSEPSGRSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPV 2460
            QIQT EPSGRSETIVENS Q   DNLPPPVA+NSSDGT+SRPA SESLQ TQV QQSQPV
Sbjct: 2401 QIQTLEPSGRSETIVENSEQQERDNLPPPVALNSSDGTDSRPAVSESLQETQVNQQSQPV 2460

Query: 2461 EMQFEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGSADRMSL 2520
            EMQFEH+DAAVRDVEAVSQESGGSGAT GESLRSLDVEIGS DGHDDGGDRQGSADRMSL
Sbjct: 2461 EMQFEHSDAAVRDVEAVSQESGGSGATLGESLRSLDVEIGSADGHDDGGDRQGSADRMSL 2520

Query: 2521 GDSQAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGLG 2580
            GDSQ ARMRRSNVSF+NSA LSGRD SLHIVTEVSENSSREADEEGPVGEQQTNSD G G
Sbjct: 2521 GDSQVARMRRSNVSFSNSAPLSGRDASLHIVTEVSENSSREADEEGPVGEQQTNSDIGSG 2580

Query: 2581 AIDPAFLDALPEELRAEVLSTQQGQVVQPPSNE--PQNSGDIDPEFLAALPPDIRAEVLA 2640
            AIDPAFLDALPEELRAEVLSTQQGQ VQPPSNE   QNSGDIDPEFLAALPPDIRAEVLA
Sbjct: 2581 AIDPAFLDALPEELRAEVLSTQQGQAVQPPSNESQSQNSGDIDPEFLAALPPDIRAEVLA 2640

Query: 2641 QQQAQRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANML 2700
            QQQAQRLH SQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANML
Sbjct: 2641 QQQAQRLHQSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANML 2700

Query: 2701 RERFAHRYHNRSLFGMHPRNRRGEITRRVEGI-SGLDRTGGSISSRRSLGSRLIEAEGAP 2760
            RERFAHRYHNR+LFGM+PRNRRGE +RRVEGI SGLDRTGGSISSRRSLG+RLIEA+GAP
Sbjct: 2701 RERFAHRYHNRTLFGMYPRNRRGESSRRVEGISSGLDRTGGSISSRRSLGARLIEADGAP 2760

Query: 2761 LVDTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQ 2820
            LVDTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMD+LLFDRRKPA+Q
Sbjct: 2761 LVDTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDMLLFDRRKPASQ 2820

Query: 2821 SNTTELSYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFF 2880
            SN+TELSYRLYACQRNVIYSRP FFDGAPPLVSRRVLETLTYLARNHP+VAKILLQ KFF
Sbjct: 2821 SNSTELSYRLYACQRNVIYSRPQFFDGAPPLVSRRVLETLTYLARNHPYVAKILLQFKFF 2880

Query: 2881 RPTLQESENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQL 2940
             PTL+ESENV Q+CGKAVMAV+ NE DCR QS G+LSIALLLSLL+QPLYLRSIAHLEQL
Sbjct: 2881 GPTLRESENVYQNCGKAVMAVEPNETDCRQQSEGFLSIALLLSLLSQPLYLRSIAHLEQL 2940

Query: 2941 LNLLEVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGS 3000
            LNLLEVI+DNAESKSHLS+Q   S  EQPAAPEVS SDAEVN +   VSSGVGTSSK+G+
Sbjct: 2941 LNLLEVIVDNAESKSHLSEQSVPSAAEQPAAPEVSSSDAEVNAEPGGVSSGVGTSSKIGA 3000

Query: 3001 YFKMAASAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPI 3060
            Y K+ ASAAD EF  HSIL NLPEAELRLLCSLLAREGLSDNTYALV EVMKKLV IAPI
Sbjct: 3001 YSKITASAADSEFGSHSILVNLPEAELRLLCSLLAREGLSDNTYALVAEVMKKLVAIAPI 3060

Query: 3061 HCRLFITELSESVEKLTRSAMDELHMFGEAVKA-LSISSSDGAAILRVLQALSSLVALLV 3120
            HCRLFITELSESV+KLTRSAMDELHMFGEAVKA LS SSSDGAAILRVLQALSSLVA LV
Sbjct: 3061 HCRLFITELSESVQKLTRSAMDELHMFGEAVKALLSTSSSDGAAILRVLQALSSLVASLV 3120

Query: 3121 EKRKDSSILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPT 3180
            EK KDSSILPEKEHASALTLV DINAALEPLWLELSTCI KIESYSDSSPD L SFRTPT
Sbjct: 3121 EKGKDSSILPEKEHASALTLVWDINAALEPLWLELSTCISKIESYSDSSPDMLASFRTPT 3180

Query: 3181 SKPTGVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQ 3240
            SKP GVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNI TVSEVEDAG SA  Q
Sbjct: 3181 SKPAGVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNITTVSEVEDAGVSAAQQ 3240

Query: 3241 RTTVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSH 3300
            RTTVPTQKVDEKH+AFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKR+H
Sbjct: 3241 RTTVPTQKVDEKHVAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRAH 3300

Query: 3301 FRSRIKHQHD-HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGL 3360
            FRS+IKHQHD HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGL
Sbjct: 3301 FRSKIKHQHDHHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGL 3360

Query: 3361 SREWYQVLSRVIFDKGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQ 3420
            SREWYQ+LSRVIFDKGALLFTTVGNDSTFQPNPNS YQTEHLSYFKFVGRVVGKALYDGQ
Sbjct: 3361 SREWYQLLSRVIFDKGALLFTTVGNDSTFQPNPNSAYQTEHLSYFKFVGRVVGKALYDGQ 3420

Query: 3421 LLDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKL 3480
            LLDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVL LTFSVDADEEKL
Sbjct: 3421 LLDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLDLTFSVDADEEKL 3480

Query: 3481 ILYEKTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPR 3540
            ILYE+TEVTDYELIPGGRN KVTEENKYQYVDLVVEHQLTTAIRPQINAFL+GFHE+IPR
Sbjct: 3481 ILYERTEVTDYELIPGGRNIKVTEENKYQYVDLVVEHQLTTAIRPQINAFLEGFHELIPR 3540

Query: 3541 ELISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARL 3600
            ELISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARL
Sbjct: 3541 ELISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARL 3600

Query: 3601 LQFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHL 3660
            LQFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHL
Sbjct: 3601 LQFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHL 3660

Query: 3661 EERLILAIHEANEGFGFG 3665
            EERL+LAIHEANEGFGFG
Sbjct: 3661 EERLLLAIHEANEGFGFG 3672

BLAST of CmoCh12G001650 vs. ExPASy TrEMBL
Match: A0A6J1HFK2 (HECT-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462592 PE=4 SV=1)

HSP 1 Score: 6412.8 bits (16636), Expect = 0.0e+00
Identity = 3339/3676 (90.83%), Postives = 3474/3676 (94.50%), Query Frame = 0

Query: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60
            MTTQRSSLPSRLRQLLSGEGSFGPS+KLDSEPPPKIKAFID+VIQCPLHDIAI LSGFRW
Sbjct: 1    MTTQRSSLPSRLRQLLSGEGSFGPSIKLDSEPPPKIKAFIDQVIQCPLHDIAIPLSGFRW 60

Query: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKD-LLSDKILEDDSPFPKHAILQILRVMQIVL 120
            +YNKGNYHHWRPLFLHFDTYFKTYLSCR D LLSDKILEDDSPFPKHAILQILRVMQIVL
Sbjct: 61   KYNKGNYHHWRPLFLHFDTYFKTYLSCRNDLLLSDKILEDDSPFPKHAILQILRVMQIVL 120

Query: 121  ENCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180
            ENCHNKGSLDGLE+F LLLASTDPE+LIAALETLSALVKINPSKLHGRGKLIGCGSVNSY
Sbjct: 121  ENCHNKGSLDGLEHFKLLLASTDPEVLIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180

Query: 181  LLSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHG 240
            LLSLAQGWGSKEEGLGLYSCVVA+E T+E+G CLFPH+VEND +NAQYRIGSSLYFELHG
Sbjct: 181  LLSLAQGWGSKEEGLGLYSCVVANERTQEKGLCLFPHEVENDMDNAQYRIGSSLYFELHG 240

Query: 241  CGAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRY 300
             GAK SDESSSSS+S NSRVIHIPDLHLKKEDDL VLKQCIELY+VPPE RFSLLTRIRY
Sbjct: 241  SGAKDSDESSSSSNSFNSRVIHIPDLHLKKEDDLVVLKQCIELYNVPPEFRFSLLTRIRY 300

Query: 301  ARAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPG 360
            ARAF SS + RLYS+ICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETV G
Sbjct: 301  ARAFRSSKVCRLYSRICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVSG 360

Query: 361  TIRTLAMRALGAQFSAY-SSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSS 420
            +IRTLAM ALGAQ +AY SSHERRILSGSSISFAGGNR ILLNVLQKAILSLKNSNDPSS
Sbjct: 361  SIRTLAMLALGAQLAAYSSSHERRILSGSSISFAGGNRMILLNVLQKAILSLKNSNDPSS 420

Query: 421  LAFVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLM 480
            LAFVEALLQFYLLH+VSSSASGNNIR SGMVPTFLPLLEDSDPSHLHLVC AVKTLQKLM
Sbjct: 421  LAFVEALLQFYLLHVVSSSASGNNIRGSGMVPTFLPLLEDSDPSHLHLVCFAVKTLQKLM 480

Query: 481  DFSSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRM 540
            DFSSSAVSLFKELGGVEILVERLQTEVHRVIGLSG N DSMIIGESSRCNDDQLYNQKR+
Sbjct: 481  DFSSSAVSLFKELGGVEILVERLQTEVHRVIGLSGTNTDSMIIGESSRCNDDQLYNQKRL 540

Query: 541  IKVSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYP 600
            IKVSLKALGVATYVPT+STN+LP ILS IFGN+DKFGGDIYCSAVTLMSEIIHKDPTCYP
Sbjct: 541  IKVSLKALGVATYVPTNSTNSLPAILSQIFGNIDKFGGDIYCSAVTLMSEIIHKDPTCYP 600

Query: 601  LLHELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFT 660
             LH++GLPDAFLASV+AGILPSPKA+TC+PNGIGAICLNARGLEAVKET ALRFLIDIFT
Sbjct: 601  SLHDMGLPDAFLASVSAGILPSPKAVTCIPNGIGAICLNARGLEAVKETSALRFLIDIFT 660

Query: 661  KEKYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLN 720
            KEKYVL MNEAIVPLANAVEEL RHVSSLRS+GVDIILE+IEKI+SLG+KDP+ SS KLN
Sbjct: 661  KEKYVLAMNEAIVPLANAVEELLRHVSSLRSTGVDIILEIIEKISSLGEKDPIGSSEKLN 720

Query: 721  GNTAMETDSDDKDNN---SIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENS 780
            GNTAMETDSDDK+NN   S+VTEE IN +QV+QLCIFHLMVLVHRTMENSETCR+FVENS
Sbjct: 721  GNTAMETDSDDKENNSNCSLVTEEGINNDQVIQLCIFHLMVLVHRTMENSETCRVFVENS 780

Query: 781  GIEALLKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGF 840
            GIEALLKLLLRP+IAQS+NGTIALHSTMVFKGFTQHHSAPLARAFCS LRDHLKK LTGF
Sbjct: 781  GIEALLKLLLRPSIAQSSNGTIALHSTMVFKGFTQHHSAPLARAFCSLLRDHLKKALTGF 840

Query: 841  DLVSGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIG 900
             L+SGSFLLDPRTTPDEKIF+SLFLVEFLLFLADSKDNRWV ALLTEFGNESK+VLEDIG
Sbjct: 841  GLISGSFLLDPRTTPDEKIFTSLFLVEFLLFLADSKDNRWVTALLTEFGNESKDVLEDIG 900

Query: 901  RVHRQILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRR 960
            RVHR+ILWQIALLEDVKP+LEDESTGS+TELQNSEV TNE+EEQRFNSFRQFLDPLL RR
Sbjct: 901  RVHREILWQIALLEDVKPDLEDESTGSVTELQNSEVQTNEVEEQRFNSFRQFLDPLL-RR 960

Query: 961  TSGWSVESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQ-RRSESSDTIGASNR 1020
            TSGWS+ESQFFDLINLYRDLGRAPSSL RMG + S  LQFGA NQ  R+ SSDT G SN 
Sbjct: 961  TSGWSIESQFFDLINLYRDLGRAPSSLHRMGSDSSSFLQFGAGNQGLRAGSSDTTGTSNG 1020

Query: 1021 KEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080
            KEYS  R CYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS
Sbjct: 1021 KEYSNQRNCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080

Query: 1081 SLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGV 1140
            SLVLDHMNF GHVNASGSEGSISTKCRYFGKVIDF+DGILLDRPDSCN VLLNCLYGHGV
Sbjct: 1081 SLVLDHMNFGGHVNASGSEGSISTKCRYFGKVIDFLDGILLDRPDSCNPVLLNCLYGHGV 1140

Query: 1141 VSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHL 1200
            V SVLTTFEATSQLLFTINR P+SPMETDDA LKQDEK DNDHSWIQGPLASYGRLMDHL
Sbjct: 1141 VHSVLTTFEATSQLLFTINRVPASPMETDDATLKQDEKADNDHSWIQGPLASYGRLMDHL 1200

Query: 1201 VTSPFILSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCE 1260
            VTS FILSSFTKHLLAQPLTSGDV FPRDAETFVKVLQSMVLKAVLPVWTHPQF+DCS E
Sbjct: 1201 VTSSFILSSFTKHLLAQPLTSGDVAFPRDAETFVKVLQSMVLKAVLPVWTHPQFLDCSSE 1260

Query: 1261 FITTVISIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVG 1320
            FITTVISIIRHIYSGVEVK+VSSNSSARI GPPPNETAISTIVEMGFSR RAEEALRQVG
Sbjct: 1261 FITTVISIIRHIYSGVEVKNVSSNSSARITGPPPNETAISTIVEMGFSRSRAEEALRQVG 1320

Query: 1321 SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCT 1380
            SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESV LPCT
Sbjct: 1321 SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVHLPCT 1380

Query: 1381 EELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNS 1440
            EELLSTC+RLLRAKE LAFPVRDLLVMICSQNDGQNRS V+SFLI+SVKGCD ++DSGNS
Sbjct: 1381 EELLSTCIRLLRAKEALAFPVRDLLVMICSQNDGQNRSKVISFLIDSVKGCDTVADSGNS 1440

Query: 1441 TTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAF 1500
            TTLSALFHVIALILNDDTVARE+AY+NGLV VSSNLLS+WDSGLS GVKVEVPKWVTAAF
Sbjct: 1441 TTLSALFHVIALILNDDTVARESAYKNGLVAVSSNLLSRWDSGLSDGVKVEVPKWVTAAF 1500

Query: 1501 LAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKEL 1560
            LAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVG QK+L
Sbjct: 1501 LAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGIQKKL 1560

Query: 1561 IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDS 1620
            IEIACSC+KKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDS
Sbjct: 1561 IEIACSCMKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDS 1620

Query: 1621 IASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIF 1680
            IASTIIRHILEDPQTLQQAMESEI+HTLITAMNRHPNGRVTPRNFLLGLNSVI+RDPVIF
Sbjct: 1621 IASTIIRHILEDPQTLQQAMESEIKHTLITAMNRHPNGRVTPRNFLLGLNSVITRDPVIF 1680

Query: 1681 MRATQSVCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANP 1740
            MRA QSVCQIEM+GERPY+VLLKDREKDKSKEKDR+KEKSMEKEK QNHDVKV LGNAN 
Sbjct: 1681 MRAAQSVCQIEMVGERPYIVLLKDREKDKSKEKDREKEKSMEKEKIQNHDVKVALGNANS 1740

Query: 1741 ATAGNVRSKFHDS--RSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQA 1800
            A  GNV SK HDS  RSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVEL   A+A
Sbjct: 1741 AVIGNVHSKPHDSNLRSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELACSARA 1800

Query: 1801 SSDMDIDVAAVKGKGKAIASLSED-DANSQEASASLAKVVFILKLLTEILLMYASSVHVL 1860
            SSDMDIDV+AVKGKGKAIASLSED DANSQEASASLAKVVFILKLLTEILLMYASSVHVL
Sbjct: 1801 SSDMDIDVSAVKGKGKAIASLSEDNDANSQEASASLAKVVFILKLLTEILLMYASSVHVL 1860

Query: 1861 IRRDTEVCCSRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGS 1920
            +R+DTEVCCSR VHQR S+GGCTGGI HHILH FIPLSRNSKKEKKVDGDWKHKLATRGS
Sbjct: 1861 LRKDTEVCCSRSVHQRGSSGGCTGGIFHHILHEFIPLSRNSKKEKKVDGDWKHKLATRGS 1920

Query: 1921 QFLVASCVRSSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTG 1980
            QFLVASCVRSSEARRRIFVEVGSILNQFID CNGSRPPSSDLQAFVDLLNDMLAARTPTG
Sbjct: 1921 QFLVASCVRSSEARRRIFVEVGSILNQFIDPCNGSRPPSSDLQAFVDLLNDMLAARTPTG 1980

Query: 1981 SYITTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNA 2040
            SYITTEASATF+DAGLV SFTQILKVLDLDHPDSPKVVTG+IKALELVTKEHV   DSN 
Sbjct: 1981 SYITTEASATFIDAGLVSSFTQILKVLDLDHPDSPKVVTGLIKALELVTKEHVQLADSNT 2040

Query: 2041 GKGDSSSKTPDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTD 2100
            GKGD+SSKTPDH+QPGGENIGETPR+MET SQSNHESIPA+QIE YNANQ Y GSEAVTD
Sbjct: 2041 GKGDNSSKTPDHNQPGGENIGETPRSMETASQSNHESIPADQIESYNANQNYVGSEAVTD 2100

Query: 2101 DMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEM 2160
            DMEHDQDL GVFG N EDE MH T E+ARGLENGIDTVDI+IEIQPH PENLDEDD+EEM
Sbjct: 2101 DMEHDQDLDGVFGPNGEDEYMHETSEDARGLENGIDTVDIRIEIQPHGPENLDEDDNEEM 2160

Query: 2161 SGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE 2220
            SGDDGDEV    DEDEDEDEEEQND E+DEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE
Sbjct: 2161 SGDDGDEV----DEDEDEDEEEQNDPEEDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE 2220

Query: 2221 DDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280
            DDEDDEDGV+LRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN
Sbjct: 2221 DDEDDEDGVILRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280

Query: 2281 LLGRTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSG 2340
            LLGRTGD VAP+RHPLLGGP LHA PFRPSENNRDMV+SER+LENNSSGLDTVFRSLRSG
Sbjct: 2281 LLGRTGDNVAPTRHPLLGGPALHAAPFRPSENNRDMVLSERTLENNSSGLDTVFRSLRSG 2340

Query: 2341 RHGHRLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNG 2400
            RHGHRLNLWAND QH+GGS+NG IPQGLEE LVSQLRRPTPEK SEL+A  EPDN+D +G
Sbjct: 2341 RHGHRLNLWANDNQHSGGSSNGAIPQGLEEFLVSQLRRPTPEKSSELNATVEPDNKDSSG 2400

Query: 2401 QIQTSEPSGRSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPV 2460
            QIQTSEPSGRSETIVE+SGQH  D+LPPP  IN  DG+NS PA  ESLQGTQV QQ+QPV
Sbjct: 2401 QIQTSEPSGRSETIVEDSGQHDRDSLPPPGPINIFDGSNSGPAVIESLQGTQVTQQTQPV 2460

Query: 2461 EMQFEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGS-ADRMS 2520
            EMQFE  DAAVRDVEAVSQESGGSGAT GESLRSLDVEIGS DGHDDGGDRQGS ADRMS
Sbjct: 2461 EMQFEPGDAAVRDVEAVSQESGGSGATLGESLRSLDVEIGSADGHDDGGDRQGSAADRMS 2520

Query: 2521 LGDSQAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGL 2580
            LGDSQAARMRR+NVS++NSA LSGRD SLHIVTEVSENSSREADEEGPVGEQQTNSDTG 
Sbjct: 2521 LGDSQAARMRRTNVSYSNSAPLSGRDASLHIVTEVSENSSREADEEGPVGEQQTNSDTGS 2580

Query: 2581 GAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQ 2640
            GAIDPAFLDALPEELRAEVLSTQQGQVVQPP+NEPQN+GDIDPEFLAALPPDIRAEVLAQ
Sbjct: 2581 GAIDPAFLDALPEELRAEVLSTQQGQVVQPPNNEPQNAGDIDPEFLAALPPDIRAEVLAQ 2640

Query: 2641 QQAQRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR 2700
            QQAQRLH SQELEGQPVEMDTVSIIATFPSDLREEVLLTSS+AILANLTPALVAEANMLR
Sbjct: 2641 QQAQRLHQSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSEAILANLTPALVAEANMLR 2700

Query: 2701 ERFAHRYHNRSLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLV 2760
            ERFAHRYHNR+LFGM+PRNRRGE +RRVEGISGLDRTGGSISSRRSLG+RLIEA+GAPLV
Sbjct: 2701 ERFAHRYHNRALFGMYPRNRRGESSRRVEGISGLDRTGGSISSRRSLGARLIEADGAPLV 2760

Query: 2761 DTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSN 2820
            DTDAL+AMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMD+LLFDRRK A+QSN
Sbjct: 2761 DTDALYAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDMLLFDRRKLASQSN 2820

Query: 2821 TTELSYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRP 2880
            +TELSYRLYACQRNVIYSRP FF+GAPPLVSRRVLETLTYLARNHP+VAKILLQ KFFRP
Sbjct: 2821 STELSYRLYACQRNVIYSRPQFFEGAPPLVSRRVLETLTYLARNHPYVAKILLQFKFFRP 2880

Query: 2881 TLQESENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLN 2940
            TL+ESE+  QDCGKAVMAV+ N      QS GYLSIALLL LLNQPLYLRSIAHLEQLLN
Sbjct: 2881 TLRESESAYQDCGKAVMAVEHN-----LQSEGYLSIALLLGLLNQPLYLRSIAHLEQLLN 2940

Query: 2941 LLEVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYF 3000
            LLEVIIDNAESKSH  +Q   ST EQPA PEVS SDAE+N  S  VSSGVGTSSK+G+Y 
Sbjct: 2941 LLEVIIDNAESKSHSPEQSAPSTAEQPAVPEVSSSDAELNADSGGVSSGVGTSSKIGAYS 3000

Query: 3001 KMAASAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHC 3060
            K  AS +DGEFD  SILA LPEAELRLLCSLLAREGLSDNTYALV EVMKKLV IAPIHC
Sbjct: 3001 KTTASTSDGEFDSRSILAKLPEAELRLLCSLLAREGLSDNTYALVAEVMKKLVAIAPIHC 3060

Query: 3061 RLFITELSESVEKLTRSAMDELHMFGEAVKA-LSISSSDGAAILRVLQALSSLVALLVEK 3120
            RLFITELSESV+KLTRSAMDELHMFGEAVKA LS SSSDGAAILRVLQALSSLVA LVEK
Sbjct: 3061 RLFITELSESVQKLTRSAMDELHMFGEAVKALLSTSSSDGAAILRVLQALSSLVASLVEK 3120

Query: 3121 RKDSSILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSK 3180
             KDSSILPEKEHASAL+LV DINAALEPLWLELSTCI KIESYSDSSPD L SFRTPT K
Sbjct: 3121 GKDSSILPEKEHASALSLVWDINAALEPLWLELSTCISKIESYSDSSPDMLASFRTPTMK 3180

Query: 3181 PTGVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRT 3240
            P GVTPPLPAGSQNILPYIESFFVVCEKLHP Q GSDQE+NIATVSEVEDA  SA  QRT
Sbjct: 3181 PAGVTPPLPAGSQNILPYIESFFVVCEKLHPTQTGSDQEVNIATVSEVEDASVSATQQRT 3240

Query: 3241 TVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFR 3300
            TVPT KVDEKH+AF+RFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFR
Sbjct: 3241 TVPTHKVDEKHVAFVRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFR 3300

Query: 3301 SRIKHQHD-HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSR 3360
            S+IKHQHD HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSR
Sbjct: 3301 SKIKHQHDHHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSR 3360

Query: 3361 EWYQVLSRVIFDKGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLL 3420
            EWYQ+LSRVIFDKGALLFTTVGNDSTFQPNPNS +QTEHLSYFKFVGRVVGKALYDGQLL
Sbjct: 3361 EWYQLLSRVIFDKGALLFTTVGNDSTFQPNPNSAFQTEHLSYFKFVGRVVGKALYDGQLL 3420

Query: 3421 DVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLIL 3480
            DVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVL LTFSVDADEEKLIL
Sbjct: 3421 DVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLDLTFSVDADEEKLIL 3480

Query: 3481 YEKTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPREL 3540
            YE+TEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHE+IPREL
Sbjct: 3481 YERTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHELIPREL 3540

Query: 3541 ISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLLQ 3600
            ISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVI WFWEVVQ+FSKEDKARLLQ
Sbjct: 3541 ISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVILWFWEVVQSFSKEDKARLLQ 3600

Query: 3601 FVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEE 3660
            FVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSP+HLPSAHTCFNQLDLPEYPSKQHLEE
Sbjct: 3601 FVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPEHLPSAHTCFNQLDLPEYPSKQHLEE 3660

Query: 3661 RLILAIHEANEGFGFG 3665
            +L+LA+HEANEGFGFG
Sbjct: 3661 KLLLAVHEANEGFGFG 3666

BLAST of CmoCh12G001650 vs. ExPASy TrEMBL
Match: A0A0A0LLS1 (HECT-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G370380 PE=4 SV=1)

HSP 1 Score: 6398.9 bits (16600), Expect = 0.0e+00
Identity = 3328/3677 (90.51%), Postives = 3477/3677 (94.56%), Query Frame = 0

Query: 1    MTTQRSSLPSRLRQLLSGEGSFGPSVKLDSEPPPKIKAFIDKVIQCPLHDIAIHLSGFRW 60
            MTTQRSSLPSRLRQLLSGEGSFGPS+KLDSEPPPKIKAFIDKVIQCPLHDIAI LSGFRW
Sbjct: 1    MTTQRSSLPSRLRQLLSGEGSFGPSIKLDSEPPPKIKAFIDKVIQCPLHDIAIPLSGFRW 60

Query: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRKD-LLSDKILEDDSPFPKHAILQILRVMQIVL 120
            EYNKGNYHHWRPLFLHFDTYFKTYLSCR D LLSDKILEDDSPFPKHAILQILRVMQIVL
Sbjct: 61   EYNKGNYHHWRPLFLHFDTYFKTYLSCRNDLLLSDKILEDDSPFPKHAILQILRVMQIVL 120

Query: 121  ENCHNKGSLDGLEYFNLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180
            ENCHNKGSLDGLE+F LLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY
Sbjct: 121  ENCHNKGSLDGLEHFKLLLASTDPEILIAALETLSALVKINPSKLHGRGKLIGCGSVNSY 180

Query: 181  LLSLAQGWGSKEEGLGLYSCVVADEITREEGRCLFPHDVENDTNNAQYRIGSSLYFELHG 240
            LLSLAQGWGSKEEGLGLYSCV+A+E T+EEG CLFPH+VEND +NAQYRIGSSLYFELHG
Sbjct: 181  LLSLAQGWGSKEEGLGLYSCVIANERTQEEGLCLFPHEVENDMDNAQYRIGSSLYFELHG 240

Query: 241  CGAKSSDESSSSSSSSNSRVIHIPDLHLKKEDDLTVLKQCIELYDVPPELRFSLLTRIRY 300
            CGAK S+ESSSSSSSSNS+VIHIPDLHL+KEDDL VLK+CIELY+VPPELRFSLLTRIRY
Sbjct: 241  CGAKDSEESSSSSSSSNSQVIHIPDLHLEKEDDLIVLKRCIELYNVPPELRFSLLTRIRY 300

Query: 301  ARAFHSSNLRRLYSKICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVPG 360
            ARAF SS + RLYS+ICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETV G
Sbjct: 301  ARAFRSSKICRLYSRICLLAFIVLVQSGDSHDELVAFFANEPEYTNELIRIVRSEETVSG 360

Query: 361  TIRTLAMRALGAQFSAY-SSHERRILSGSSISFAGGNRTILLNVLQKAILSLKNSNDPSS 420
            +IRTLAM ALGAQ +AY SSHERRILSGSSISFAGGNR ILLNVLQKAILSLKNSNDPSS
Sbjct: 361  SIRTLAMLALGAQLAAYSSSHERRILSGSSISFAGGNRMILLNVLQKAILSLKNSNDPSS 420

Query: 421  LAFVEALLQFYLLHMVSSSASGNNIRSSGMVPTFLPLLEDSDPSHLHLVCLAVKTLQKLM 480
            LAF+EALLQFYLLH+VSSSASGNNIR SGMVPTFL LLEDSDP+HLHLVC AVKTLQKLM
Sbjct: 421  LAFIEALLQFYLLHVVSSSASGNNIRGSGMVPTFLTLLEDSDPTHLHLVCFAVKTLQKLM 480

Query: 481  DFSSSAVSLFKELGGVEILVERLQTEVHRVIGLSGNNIDSMIIGESSRCNDDQLYNQKRM 540
            DFSSS+VSLFKELGGVEILVERLQTEV+RVIGLSG NIDSMIIGESS+CNDDQLYNQKR+
Sbjct: 481  DFSSSSVSLFKELGGVEILVERLQTEVNRVIGLSGANIDSMIIGESSKCNDDQLYNQKRL 540

Query: 541  IKVSLKALGVATYVPTSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYP 600
            IKV+LKALGVATYVPT+STN+LP ILS IFGN+DKFGGDIYCSAVTLMSEIIHKDPTCYP
Sbjct: 541  IKVALKALGVATYVPTNSTNSLPVILSQIFGNIDKFGGDIYCSAVTLMSEIIHKDPTCYP 600

Query: 601  LLHELGLPDAFLASVAAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFT 660
             LH++GLPDAFLASVAAGILPSPKA+TCVPNGIGAICLNARGLEAVKET ALRFLID+FT
Sbjct: 601  SLHDMGLPDAFLASVAAGILPSPKAVTCVPNGIGAICLNARGLEAVKETSALRFLIDVFT 660

Query: 661  KEKYVLVMNEAIVPLANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLN 720
            KEKYVL +NEAIVPLANAVEEL RHVSSLRS+GVDIILEVIEK+TSLG+KDP+ SSGKLN
Sbjct: 661  KEKYVLAVNEAIVPLANAVEELLRHVSSLRSTGVDIILEVIEKVTSLGEKDPIGSSGKLN 720

Query: 721  GNTAMETDSDDKDNN---SIVTEEVINTEQVVQLCIFHLMVLVHRTMENSETCRIFVENS 780
            GNTAMETDSDDK+NN   S+VTEE I+ EQV+QLCI HLMVLVHRTMENSETCRIFVENS
Sbjct: 721  GNTAMETDSDDKENNSNCSLVTEEGISNEQVIQLCICHLMVLVHRTMENSETCRIFVENS 780

Query: 781  GIEALLKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGF 840
            GIEALLKLLLRP+IAQS+NGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKK LTGF
Sbjct: 781  GIEALLKLLLRPSIAQSSNGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKALTGF 840

Query: 841  DLVSGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIG 900
            DL+SGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWV ALLTEFGNESK+VLEDIG
Sbjct: 841  DLISGSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVTALLTEFGNESKDVLEDIG 900

Query: 901  RVHRQILWQIALLEDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFRQFLDPLLRRR 960
            RVHR+ILWQIALLED+KPELEDESTGS+T+LQNSEVHTNEIEEQRFNSFRQFLDPLLRRR
Sbjct: 901  RVHREILWQIALLEDIKPELEDESTGSVTDLQNSEVHTNEIEEQRFNSFRQFLDPLLRRR 960

Query: 961  TSGWSVESQFFDLINLYRDLGRAPSSLQRMGPNGSPLLQFGANNQ-RRSESSDTIGASNR 1020
            TSGWS+ESQFFDLINLYRDLGRAPSSLQRM  + S LLQFG  NQ  R+ SSDT G SN 
Sbjct: 961  TSGWSIESQFFDLINLYRDLGRAPSSLQRMSSDSSSLLQFGVGNQGLRAGSSDTTGTSNE 1020

Query: 1021 KEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080
            KE S  R C+ASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS
Sbjct: 1021 KECSNQRNCHASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTLS 1080

Query: 1081 SLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNTVLLNCLYGHGV 1140
            SLVLDHMNF GHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCN VLLNCLYGHGV
Sbjct: 1081 SLVLDHMNFGGHVNASGSEGSISTKCRYFGKVIDFVDGILLDRPDSCNPVLLNCLYGHGV 1140

Query: 1141 VSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHSWIQGPLASYGRLMDHL 1200
            V SVLTTFEATSQLLFTINR P+SPMETDDANLKQ+EK DNDHSWIQGPLASYGRLMDHL
Sbjct: 1141 VQSVLTTFEATSQLLFTINRTPASPMETDDANLKQEEKADNDHSWIQGPLASYGRLMDHL 1200

Query: 1201 VTSPFILSSFTKHLLAQPLTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCE 1260
            VTSPFILSSFTKHLLAQ LTSGD+ FPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCE
Sbjct: 1201 VTSPFILSSFTKHLLAQSLTSGDIAFPRDAETFVKVLQSMVLKAVLPVWTHPQFIDCSCE 1260

Query: 1261 FITTVISIIRHIYSGVEVKSVSSNSSARIAGPPPNETAISTIVEMGFSRGRAEEALRQVG 1320
            FITTVISIIRHIYSGVEVK+VSSNSSAR+ GPPPNET ISTIVEMGFSR RAEEALRQVG
Sbjct: 1261 FITTVISIIRHIYSGVEVKNVSSNSSARMTGPPPNETTISTIVEMGFSRSRAEEALRQVG 1320

Query: 1321 SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVQLPCT 1380
            SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESV LPCT
Sbjct: 1321 SNSVELAMEWLFSHPEEVQEDDELARALALSLGNSELEMKEPVSSEVSKQIEESVHLPCT 1380

Query: 1381 EELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFLIESVKGCDKISDSGNS 1440
            EELLSTC++LLRAKE LAFPVRDLLVMICSQNDGQNRSNV+SFLI+SVKGCD ++DSGNS
Sbjct: 1381 EELLSTCIKLLRAKEALAFPVRDLLVMICSQNDGQNRSNVISFLIDSVKGCDTVADSGNS 1440

Query: 1441 TTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGLSFGVKVEVPKWVTAAF 1500
            TTLSALFHVIALILNDDTVAR+AAY+NGLV VSSNLLS+WD+G S GVKVEVPKWVTAAF
Sbjct: 1441 TTLSALFHVIALILNDDTVARDAAYKNGLVAVSSNLLSRWDTGFSDGVKVEVPKWVTAAF 1500

Query: 1501 LAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDEDKQNKLQSALGLSPKYIDVGNQKEL 1560
            LAIDRLLQEEKKFNPEIADQLKRD+ GGDTLTIDEDKQ KLQSALGLSPKYIDVG+QK+L
Sbjct: 1501 LAIDRLLQEEKKFNPEIADQLKRDHGGGDTLTIDEDKQTKLQSALGLSPKYIDVGSQKKL 1560

Query: 1561 IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDS 1620
            IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAV FLEAGGLTSLLSLPTTSLFPGFDS
Sbjct: 1561 IEIACSCIKKRLPCETMHAVLQLCSSLTRSHSVAVCFLEAGGLTSLLSLPTTSLFPGFDS 1620

Query: 1621 IASTIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIF 1680
            IAS+IIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVI+RDPVIF
Sbjct: 1621 IASSIIRHILEDPQTLQQAMESEIRHTLITAMNRHPNGRVTPRNFLLGLNSVITRDPVIF 1680

Query: 1681 MRATQSVCQIEMIGERPYVVLLKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANP 1740
            MRA QSVCQIEM+GERPY+VLLKDREKDKSKEKDR+KEK MEKEK  NHDVKV LGN N 
Sbjct: 1681 MRAAQSVCQIEMVGERPYIVLLKDREKDKSKEKDREKEKLMEKEKLHNHDVKVSLGNVNS 1740

Query: 1741 ATAGNVRSKFHDS--RSSRVHKKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQA 1800
               GNV SK HDS  +SSRV+KK SQNF+NVIELLLESVYTFIPP+KDDVT ELT  A+A
Sbjct: 1741 TVVGNVHSKLHDSNLKSSRVNKKFSQNFVNVIELLLESVYTFIPPVKDDVTTELTCSARA 1800

Query: 1801 SSDMDIDVAAVKGKGKAIASLSED-DANSQEASASLAKVVFILKLLTEILLMYASSVHVL 1860
            SSDMDIDV+AVKGKGKAIASLS+D DANSQEASASLAKVVFILKLLTEILLMYASSVHVL
Sbjct: 1801 SSDMDIDVSAVKGKGKAIASLSDDNDANSQEASASLAKVVFILKLLTEILLMYASSVHVL 1860

Query: 1861 IRRDTEVCCSRPVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKVDGDWKHKLATRGS 1920
            +R+DTEVCCSRPVHQR +NGGCTGGI HHILH FIPLSRNSKK+KKVDGDWKHKLATRGS
Sbjct: 1861 LRKDTEVCCSRPVHQR-ANGGCTGGIFHHILHEFIPLSRNSKKDKKVDGDWKHKLATRGS 1920

Query: 1921 QFLVASCVRSSEARRRIFVEVGSILNQFIDSCNGSRPPSSDLQAFVDLLNDMLAARTPTG 1980
            QFLVASCVRSSEARRRIFVEVGS+LNQFIDSCN SRPP+SDLQAFVDLLNDMLAARTPTG
Sbjct: 1921 QFLVASCVRSSEARRRIFVEVGSMLNQFIDSCNSSRPPNSDLQAFVDLLNDMLAARTPTG 1980

Query: 1981 SYITTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTKEHVHFGDSNA 2040
            SYITTEASATF+DAGLV SFTQILKVLDLDHPDSPKVVTG+IKALE+VTKEHV F DSN 
Sbjct: 1981 SYITTEASATFIDAGLVSSFTQILKVLDLDHPDSPKVVTGLIKALEMVTKEHVQFADSNT 2040

Query: 2041 GKGDSSSKTPDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGGSEAVTD 2100
            GKGDSSSKTPDH+QPGGENIGETPR+MET SQSNHE IP +QIE YNANQ YGGSEAVTD
Sbjct: 2041 GKGDSSSKTPDHNQPGGENIGETPRSMETASQSNHELIPGDQIESYNANQNYGGSEAVTD 2100

Query: 2101 DMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEM 2160
            DMEHDQDL GVFG NA DE MH TPE+ARGLENGIDTVDI+IEIQPHVPENLDEDDDEEM
Sbjct: 2101 DMEHDQDLDGVFGPNAGDEYMHDTPEDARGLENGIDTVDIRIEIQPHVPENLDEDDDEEM 2160

Query: 2161 SGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE 2220
            SGDDGDEV    DEDEDEDEEEQNDLE+DEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE
Sbjct: 2161 SGDDGDEV----DEDEDEDEEEQNDLEEDEVHHLPHPDTDHDDHEIDDDEFDEVLEEDDE 2220

Query: 2221 DDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280
            DDEDDEDGV+LRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN
Sbjct: 2221 DDEDDEDGVILRLEEGINGINVFDHVEVFGRDTSQNETLHVMPVEIFGSRRQGRTTSIYN 2280

Query: 2281 LLGRTGDTVAPSRHPLLGGPGLHATPFRPSENNRDMVISERSLENNSSGLDTVFRSLRSG 2340
            LLGRTGD VAPSRHPLLGGP LHA PFRPSENNRDMVISER+LENNSSGLDTVFRSLRSG
Sbjct: 2281 LLGRTGDNVAPSRHPLLGGPALHAAPFRPSENNRDMVISERTLENNSSGLDTVFRSLRSG 2340

Query: 2341 RHGHRLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNG 2400
            RHGHRLNLWAND QH GGS+NGVIPQGLEELLVSQLRRPTPEK +EL+A  EPDN+DG G
Sbjct: 2341 RHGHRLNLWANDNQHGGGSSNGVIPQGLEELLVSQLRRPTPEKSTELNAAVEPDNKDGTG 2400

Query: 2401 QIQTSEPSGRSETIVENSGQHGNDNLPPPVAINSSDGTNSRPAASESLQGTQVIQQSQPV 2460
            QIQTSEP G SETI+ENSGQH  D LPP  A +SSDGT+S PA  ESLQGTQV QQSQ V
Sbjct: 2401 QIQTSEPVGSSETIIENSGQHDRDGLPPLAASHSSDGTSSGPAVIESLQGTQVTQQSQAV 2460

Query: 2461 EMQFEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQGS-ADRMS 2520
            +MQFEH+DAAVRDVEAVSQESGGSGAT GESLRSLDVEIGS DGHDD GDRQGS ADRMS
Sbjct: 2461 DMQFEHSDAAVRDVEAVSQESGGSGATLGESLRSLDVEIGSADGHDDSGDRQGSAADRMS 2520

Query: 2521 LGDSQAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQTNSDTGL 2580
            LGDSQAAR+RRSNVS++NS  LSGRD SLHIVTEVSENSSREADEEGPVGEQQTNS+TG 
Sbjct: 2521 LGDSQAARLRRSNVSYSNSTPLSGRDASLHIVTEVSENSSREADEEGPVGEQQTNSETGS 2580

Query: 2581 GAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIRAEVLAQ 2640
            GAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQN+GDIDPEFLAALPPDIRAEVLAQ
Sbjct: 2581 GAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNAGDIDPEFLAALPPDIRAEVLAQ 2640

Query: 2641 QQAQRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR 2700
            QQAQRLH SQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR
Sbjct: 2641 QQAQRLHQSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVAEANMLR 2700

Query: 2701 ERFAHRYHNRSLFGMHPRNRRGEITRRVEGISGLDRTGGSISSRRSLGSRLIEAEGAPLV 2760
            ERFAHRYHNR+LFGM+PRNRRGE +RRVEGISGLDRTGGSISSRRSLG+RLIEA+GAPLV
Sbjct: 2701 ERFAHRYHNRTLFGMYPRNRRGESSRRVEGISGLDRTGGSISSRRSLGARLIEADGAPLV 2760

Query: 2761 DTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSN 2820
            DTDALH+MIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMD+LLFDRRK  +QSN
Sbjct: 2761 DTDALHSMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDMLLFDRRKLTDQSN 2820

Query: 2821 TTELSYRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRP 2880
            +TELSYRL+ACQRNVIYSRP FFDGAPPLVSRRVLETLTYLARNHP+VAKILLQ KF +P
Sbjct: 2821 STELSYRLFACQRNVIYSRPQFFDGAPPLVSRRVLETLTYLARNHPYVAKILLQFKFLKP 2880

Query: 2881 TLQESENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLN 2940
            TLQ SENV +DCGKA MAV++N      Q+ GYLSIALLL LLNQPLYLRSIAHLEQLLN
Sbjct: 2881 TLQGSENVYRDCGKAAMAVEQN-----LQAEGYLSIALLLGLLNQPLYLRSIAHLEQLLN 2940

Query: 2941 LLEVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYF 3000
            LLEVIIDNAESKSHLS+Q   ST EQPAAPEVS SDAEVN  S  VSSGVGTS+K+G   
Sbjct: 2941 LLEVIIDNAESKSHLSEQSAPSTAEQPAAPEVSSSDAEVNADSGGVSSGVGTSAKIGG-S 3000

Query: 3001 KMAASAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHC 3060
            K  ASAA+ E D  SILANLPEAELRLLCSLLAREGLSDNTYALV EVMKKLV I+PIHC
Sbjct: 3001 KTTASAANSECDSQSILANLPEAELRLLCSLLAREGLSDNTYALVAEVMKKLVAISPIHC 3060

Query: 3061 RLFITELSESVEKLTRSAMDELHMFGEAVKA-LSISSSDGAAILRVLQALSSLVALLVEK 3120
            RLFITELSESV+KLTRSAMDEL MFGEAVKA LS +SSDGAAILRVLQALSSLVA L+EK
Sbjct: 3061 RLFITELSESVQKLTRSAMDELRMFGEAVKALLSTTSSDGAAILRVLQALSSLVASLIEK 3120

Query: 3121 RKDSSILPEKEHASALTLVCDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSK 3180
             KDSSILPEKEHASAL+LV DINAALEPLWLELSTCI KIESYSDSSPD L SFR PT+K
Sbjct: 3121 GKDSSILPEKEHASALSLVWDINAALEPLWLELSTCISKIESYSDSSPDVLASFRAPTAK 3180

Query: 3181 PTGVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVL-QR 3240
            P GVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIA VSEVE+AG SAV  QR
Sbjct: 3181 PAGVTPPLPAGSQNILPYIESFFVVCEKLHPAQPGSDQELNIAAVSEVEEAGVSAVAQQR 3240

Query: 3241 TTVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHF 3300
            TTVPTQKVDEKH+AF+RFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKR+HF
Sbjct: 3241 TTVPTQKVDEKHVAFVRFSEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRAHF 3300

Query: 3301 RSRIKHQHD-HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLS 3360
            RS+IKHQHD HHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLS
Sbjct: 3301 RSKIKHQHDHHHSPLRISVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLS 3360

Query: 3361 REWYQVLSRVIFDKGALLFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQL 3420
            REWYQ+LSRVIFDKGALLFTTVGNDSTFQPNPNS YQTEHLSYFKFVGRVVGKALYDGQL
Sbjct: 3361 REWYQLLSRVIFDKGALLFTTVGNDSTFQPNPNSAYQTEHLSYFKFVGRVVGKALYDGQL 3420

Query: 3421 LDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLI 3480
            LDVHFTRSFYKHILG KVTYHDIEAIDPDYYKNLKWMLENDISDVL LTFSVDADEEKLI
Sbjct: 3421 LDVHFTRSFYKHILGAKVTYHDIEAIDPDYYKNLKWMLENDISDVLDLTFSVDADEEKLI 3480

Query: 3481 LYEKTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHEIIPRE 3540
            LYE+TEVTDYELIPGGRN KVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHE+IPRE
Sbjct: 3481 LYERTEVTDYELIPGGRNIKVTEENKYQYVDLVVEHQLTTAIRPQINAFLDGFHELIPRE 3540

Query: 3541 LISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQAFSKEDKARLL 3600
            LISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQ+FSKEDKARLL
Sbjct: 3541 LISIFNDKELELLICGLPDIDLDDMRANTEYSGYSAASPVIQWFWEVVQSFSKEDKARLL 3600

Query: 3601 QFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLE 3660
            QFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLE
Sbjct: 3601 QFVTGTSKVPLEGFSALQGISGSQKFQIHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLE 3660

Query: 3661 ERLILAIHEANEGFGFG 3665
            ERL+LAIHEANEGFGFG
Sbjct: 3661 ERLLLAIHEANEGFGFG 3666

BLAST of CmoCh12G001650 vs. TAIR 10
Match: AT1G55860.2 (ubiquitin-protein ligase 1 )

HSP 1 Score: 2213.3 bits (5734), Expect = 0.0e+00
Identity = 1525/3839 (39.72%), Postives = 2175/3839 (56.66%), Query Frame = 0

Query: 30   SEPPPKIKAFIDKVIQCPLHDIAIHLSGFRWEYNKGNYHHWRPLFLHFDTYFKTYLSCRK 89
            SE P KIK+FI+ V   PL  I   L+ FRWE++KG++HHW  LF +FDT+F+ ++  RK
Sbjct: 9    SEVPSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDTFFEKHVQVRK 68

Query: 90   DL-LSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLE-YFNLLLASTDPEILI 149
            DL + +   E D PFPK A+LQ+LRV+++VLENC NK      E + +LLLASTD +++ 
Sbjct: 69   DLHIEENFEESDPPFPKDAVLQVLRVIRVVLENCTNKHFYSSYEQHLSLLLASTDADVVE 128

Query: 150  AALETLSALVKINPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCVVADEITR 209
            A L+TL+A +K    K   R       S+NS L SLAQGWG KEEGLGL SC        
Sbjct: 129  ACLQTLAAFLKRQIGKYSIRD-----ASLNSKLFSLAQGWGGKEEGLGLTSCA------- 188

Query: 210  EEGRCLFPHDVENDTNNAQYRIGSSLYFELHGCGAKSSDESSSSSSSSNSRVIHIPDLHL 269
                       EN  +    ++G +L+FE +      SDE S S      +VIH+PD+ +
Sbjct: 189  ----------AENSCDQVSLQLGRTLHFEFY-----PSDE-SPSELPGGLQVIHVPDVSI 248

Query: 270  KKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLAFIVLVQSG 329
              E DL +L + +  ++VPP LRF+LLTR+R+ARAF S   R+ ++ I L AF+VLVQ+ 
Sbjct: 249  CAESDLELLNKLVIDHNVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQAS 308

Query: 330  DSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSHERRILSGS 389
               + +V+FF  EPE+ NEL+ +V  E+TVP  IR L + +L A  S   + +  +L+  
Sbjct: 309  GDTENVVSFFNGEPEFVNELVTLVSYEDTVPEKIRILCLLSLVA-LSQDRTRQPTVLTAV 368

Query: 390  SISFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLHMVSSSASGNNIRSSG 449
            +   +GG+R +L  ++QKAI S+       SLAF EALL    + +VSSS+  + +R +G
Sbjct: 369  T---SGGHRGLLSGLMQKAIDSVVCITSKWSLAFAEALLSLVTV-LVSSSSGCSAMREAG 428

Query: 450  MVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKELGGVEILVERLQTEVHR 509
            ++PT +PL++D+DP HLHLV  AV  L+  MD+S+ A +LF++LGG++  + RL+ EV R
Sbjct: 429  LIPTLVPLIKDTDPQHLHLVSAAVHILEAFMDYSNPAAALFRDLGGLDDTIFRLKLEVSR 488

Query: 510  VIGLSGNNIDSMIIGESSRCNDDQL--------YNQKRMIKVSLKALGVATYVP------ 569
                  +  +     +S+  + +QL        Y+++ ++K  L+A+ + TY P      
Sbjct: 489  T---EDDVKEKNCSSDSNGPDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLY 548

Query: 570  TSSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLLHELGLPDAFLASV 629
             S  + LP+ L +IF     FGG ++  A T+MS++IHKDPTC+  L   GL   FL ++
Sbjct: 549  GSEESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSTFLDAI 608

Query: 630  AAGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKEKYVLVM-NEAIVP 689
            +  ++ S +AITC+P  + A+CLN  GL+AVK+  ALR  + IFT   Y+  +  +    
Sbjct: 609  SDEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFTSPSYLRALTGDTPGS 668

Query: 690  LANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGNTA-----METDS 749
            L++ ++EL RH SSLR+ GVD+ +E++  +  +G      +S   +  T+     ME D 
Sbjct: 669  LSSGLDELLRHQSSLRTYGVDMFIEILNSMLIIGSGMEATTSKSADVPTSAAPVPMEIDV 728

Query: 750  DDKDNNSIVTEEV--------INTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEA 809
            D+K  +  V++E          N E  +  C+ ++  L    ++N+E C +FVE  GI+A
Sbjct: 729  DEK--SLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDA 788

Query: 810  LLKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVS 869
            +L+L   P +  S   ++    ++ FK F+  HSA LAR  CS LR+HLKK       + 
Sbjct: 789  VLQLFSLPLMPLST--SLGQSFSVAFKNFSPQHSAGLARIVCSYLREHLKKTKILLVSIE 848

Query: 870  GSFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHR 929
            G+ LL   +    KI  SL  +E +L L++    +  A++++E      +VL+++G  ++
Sbjct: 849  GTQLLKLESAIQTKILRSLSCLEGMLSLSNFL-LKGSASVISELSAADADVLKELGITYK 908

Query: 930  QILWQIALLEDVK-PELEDESTGSITELQ-NSEVHTNEIEEQRFNSFR-QFLDPLLRRRT 989
            Q +WQ+AL  D K  E +    GS   +  +S     E +E   N+   ++ +P+  R +
Sbjct: 909  QTIWQMALCNDTKEDEKKSVDRGSDNSVSASSSTAERESDEDSSNALAVRYTNPVSIRSS 968

Query: 990  SG---WSVESQFFDLINLYRDL-GRAPSSLQRM--GPNGSPLLQFGANNQRRSESSDTIG 1049
            S    W  + +F  ++     + GR   ++ RM  G     L  F  +    SE    + 
Sbjct: 969  SSQSIWGGDREFLSIVRSGEGIHGRTRHAIARMRGGRTRRHLESFNFD----SEIPADLP 1028

Query: 1050 ASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVA 1109
             ++     K ++      +++  L+         L K     +RRR D  ++SS+SK + 
Sbjct: 1029 VTSSSHELKKKSTEVLIAEILNKLNCTLRFFFTALVKGFTSANRRRIDGASLSSASKTLG 1088

Query: 1110 STLSSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLD-RPDSCNTVLLNCL 1169
            + L+ + L+ +NF G+  A+G E S+S KCRY GKV+D +  +  D R   C T ++N  
Sbjct: 1089 TALAKVFLEALNFDGYGAAAGHEKSLSVKCRYLGKVVDDITFLSFDTRRRVCFTAMVNSF 1148

Query: 1170 YGHGVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHS-WIQGPLASYG 1229
            Y HG    +LTTFEATSQLL+T+   P S   +   N K  E+    HS W+   L +Y 
Sbjct: 1149 YVHGTFKELLTTFEATSQLLWTV---PFSIPASSTENEKPGERNIWSHSKWLVDTLQNYC 1208

Query: 1230 RLMDHLVTSPFILSSFTK-HLLAQP----LTSGDVPFPRDAETFVKVLQSMVLKAVLPVW 1289
            R +D+ V S ++LS  ++  LL QP    L+ G  P PR+ ETFV+ LQS VL  +LP+W
Sbjct: 1209 RALDYFVNSTYLLSPTSQTQLLVQPASVGLSIGLFPVPREPETFVRNLQSQVLDVILPIW 1268

Query: 1290 THPQFIDCSCEFITTVISIIRHIYSGV-----EVKSVSSNSSARIAGPPPNETAISTIVE 1349
             HP F DC+  F+ +V S++ HIYSGV         V+   + R      +E+ +  IVE
Sbjct: 1269 NHPMFPDCNPNFVASVTSLVTHIYSGVVDARENRSGVTRGINQRALPLQLDESIVGMIVE 1328

Query: 1350 MGFSRGRAEEALRQVGSNSVELAMEWLFSHPEE-VQEDDELARALALSLGN-SELEMKEP 1409
            MGFSR RAE ALR+VG+NSVE+AM+WLF++PE+ VQEDDELA+ALALSLGN SE    E 
Sbjct: 1329 MGFSRSRAEIALRRVGTNSVEMAMDWLFTNPEQPVQEDDELAQALALSLGNSSETPKLED 1388

Query: 1410 VSSEVS-KQIEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVM 1469
                V   Q E   + P  +E+++  V+L ++ +++AFP+ DL V +C++N G++R  ++
Sbjct: 1389 TEKPVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSMAFPLMDLFVTLCNRNKGEDRPKIV 1448

Query: 1470 SFLIESVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWD 1529
            S+LI+ +K   ++  S ++  L+ + H++AL+L++D   RE A Q+G+VTV+  +L+ ++
Sbjct: 1449 SYLIQQLK-LVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVTVAIGILTDFN 1508

Query: 1530 SGLSFGVKVEVPKWVTAAFLAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTID--EDKQN 1589
                   ++  PK ++A  L +  +LQ + K + E  +     N GG  +  D  +D   
Sbjct: 1509 LKSESETEILAPKCISALLLVLSMMLQAQTKLSSEYVE----GNQGGSLVPSDSPQDSTA 1568

Query: 1590 KLQSAL-----------------GLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVLQ 1649
             L+ AL                 G S  Y+ +    + + IAC  IK+ +P   M AVLQ
Sbjct: 1569 ALKDALSSDVAKGESNQALELIFGKSTGYLTMEEGHKALLIACGLIKQHVPAMIMQAVLQ 1628

Query: 1650 LCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDPQTLQQAMES 1709
            LC+ LT+SH++A+ FLE GGL+SL +LP    FPG+D++AS I+RH++EDPQTLQ AME+
Sbjct: 1629 LCARLTKSHALAIQFLENGGLSSLFNLPKKCCFPGYDTVASVIVRHLVEDPQTLQIAMET 1688

Query: 1710 EIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIEMIGERPYVVLL 1769
            EIR TL  +  RH  GRV PR FL  +  VISRDPV+FM+A  S CQ+E  G R +V+L 
Sbjct: 1689 EIRQTL--SGKRH-IGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGRDFVILS 1748

Query: 1770 KDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD--SRSSRVHK 1829
            K++EK K    +                +  PLG +         +K HD   + S+ H+
Sbjct: 1749 KEKEKPKVSGSE------------HGFSLNEPLGIS--------ENKLHDVSGKCSKSHR 1808

Query: 1830 KSSQNFINVIELLLESVYTFIPPMK--DDVTVELTSGAQASSDMDIDVAAVKGKGKAIAS 1889
            +   NFI VI+ L++ V +F P +K  +D    L S       M++D    K KGK+   
Sbjct: 1809 RVPANFIQVIDQLIDLVLSF-PRVKRQEDGETNLIS-------MEVDEPTTKVKGKSKVG 1868

Query: 1890 LSEDDANSQ----------EASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSR 1949
              E  ++S+          E S  LA+V FILKLL++I+LMY+    V++RRDTE+   R
Sbjct: 1869 EPEKASSSRVGEPEKAEIPEKSEELARVTFILKLLSDIVLMYSHGTSVILRRDTEISQLR 1928

Query: 1950 PVHQRESNGGCTGGIIHHILHGFIPLSRNSKKEKKV-DGDWKHKLATRGSQFLVASCVRS 2009
              +  + + G  GG+I+H++H  +P+S     EK V   +WK KL+ + S FLV  C RS
Sbjct: 1929 GSNLPDDSPG-NGGLIYHVIHRLLPIS----LEKFVGPEEWKEKLSEKASWFLVVLCSRS 1988

Query: 2010 SEARRRIFVEVGSILNQFIDSCNGSRP----PSSDLQAFVDLLNDMLAARTPT------G 2069
            +E R+RI  E+  +L+ F      S      P   + AF +L+  +L   + +      G
Sbjct: 1989 NEGRKRIINELSRVLSVFASLGRSSSKSVLLPDKRVLAFANLVYSILTKNSSSSSSNFPG 2048

Query: 2070 SYITTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTK-----EHVHF 2129
               + + + + +D G ++  T IL V+DLDHPD+PK+VT ++K+LE +T+     E +  
Sbjct: 2049 CGCSPDVAKSMMDGGTIQCLTSILHVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKS 2108

Query: 2130 GDSNAGKGDSSSKTPDHH----QPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQI 2189
               N  K   S +  D H    +   + + +   +++ V+ +        Q+   +A + 
Sbjct: 2109 EVPNEKKNRDSDERHDSHGNSTETEADELNQNNSSLQQVTDAAGNGQEQAQVSSQSAGE- 2168

Query: 2190 YGGSEAVTDDMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPEN 2249
             G S+  T  M  D  + G      E   M    EE  G          QIE+  HV   
Sbjct: 2169 RGSSQ--TQAMPQDMRIEGDETILPEPIQMDFMREEIEG---------DQIEMSFHVENR 2228

Query: 2250 LDEDDDEEMSGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEID--DD 2309
             D+D D++M    GDE ++DE +DED       DL +D    +    TD +D E     D
Sbjct: 2229 ADDDVDDDM----GDEGEDDEGDDEDA------DLVEDGAGVMSLAGTDVEDPEDTGLGD 2288

Query: 2310 EFDEVLEEDDEDDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQN---ETLHVMPVE- 2369
            E+++ + ++D+DD  +   + +R  E ++G+   DH ++ GR    N   + +   P E 
Sbjct: 2289 EYNDDMVDEDDDDFHENRVIEVRWREALDGL---DHFQILGRSGGGNGFIDDITAEPFEG 2348

Query: 2370 -----IFGSRR----QGRTTSIYNLLGRTGDTVAPSRHPLLGGPG--------------- 2429
                 +F  RR    + R  +  + L R+G  V   +HPL   P                
Sbjct: 2349 VNVDDLFALRRPLGFERRRQTGRSSLDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSI 2408

Query: 2430 ---------------LHATPFRPSENNRDMVISERSLENNSSG--LDTVFRSLRSGRHGH 2489
                           +  TP  P +       S R     +     D     + S R G 
Sbjct: 2409 SRHSEAGSYDVAQFYMFDTPVLPFDQVPVDPFSARLAGGGAPPPLTDYSVVGMDSSRRGV 2468

Query: 2490 RLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQT 2549
              + W +       S +  I Q +EE  +S LR   P        N   +      +IQ 
Sbjct: 2469 GDSRWTDIGHPQPSSLSASIAQLIEEHFISNLRASAP-------VNTVVERETNTTEIQE 2528

Query: 2550 S-----EPSGRSETIVENSGQHG-------------NDNLPPPVAINSSDGTNSRPAA-- 2609
                   PS  SET++ +  + G             N N PP V   S     +  A+  
Sbjct: 2529 QLHPDVPPSVGSETVLGDGNEGGQQSEERELLNNNENVNNPPDVMAESFAQGQANLASPV 2588

Query: 2610 ----SESLQGTQVIQ----QSQPVE---MQFEHNDAAVRDV---EAVSQESGGSGATFGE 2669
                 ESLQ  +V+Q     S P E   M+    D A  D    EAV   S   G     
Sbjct: 2589 SQDTGESLQQLEVMQPLPLNSTPNEIDRMEVGEGDGAPIDQVDHEAVHLISTAQGQPDTS 2648

Query: 2670 SLRSLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMRRSNVSFTNSASLSGRDTSLHI 2729
            S++++ V   +    D   + Q S D     D                + L G +  L  
Sbjct: 2649 SIQNVSVTAIAPPVDDPDSNFQPSVDVDMSSDGAEGNQS------VQPSPLDGDNNELSS 2708

Query: 2730 VTEVSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDALPEELRAEVLSTQQGQVVQPP 2789
            +       + E  EEG +  +       + AIDP FL+ALPE+LRAEVL++QQ Q VQPP
Sbjct: 2709 MEATENVRNDEQVEEGSLDGRAPE----VNAIDPTFLEALPEDLRAEVLASQQAQSVQPP 2768

Query: 2790 SNEPQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQELEGQPVEMDTVSIIATFPSD 2849
            + EP    DIDPEFLAALPPDI+ EVLAQQ+AQR  + Q+ +GQ V+MD  SIIAT P+D
Sbjct: 2769 TYEPPPVDDIDPEFLAALPPDIQTEVLAQQRAQR--MVQQSQGQAVDMDNASIIATLPAD 2828

Query: 2850 LREEVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR-SLFGMHPR--NRRGEITRRV 2909
            LREEVLLTSS+A+LA L   L+AEA MLR+R    Y  R S+FG   R  NRR  +    
Sbjct: 2829 LREEVLLTSSEAVLAALPSPLLAEAQMLRDRAMSHYQARSSVFGSSHRLNNRRNGL--GY 2888

Query: 2910 EGISGLDRTGGSISSRRSL-----GSRLIEAEGAPLVDTDALHAMIRLLRVVQPLYKGQL 2969
              ++G+DR  G    +R++     G ++ E EG PLV+ DAL ++IRLLR+ QPL KG L
Sbjct: 2889 NRLTGMDRGVGVTIGQRAVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLL 2948

Query: 2970 QRLLLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELS----YRLYACQRNVIYSR 3029
            QRLLLNLCAH+ TR +LV++L+D++     +P  +++ +EL+     RLY CQ NV+Y R
Sbjct: 2949 QRLLLNLCAHSFTRANLVQLLLDMI-----RPEMETSPSELAITNPQRLYGCQSNVVYGR 3008

Query: 3030 PSFFDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAV 3089
                +G PPLV RRVLE LTYLA NH  VA +L    +F  +L    +  +   K     
Sbjct: 3009 SQLLNGLPPLVFRRVLEVLTYLATNHSAVADMLF---YFDSSLLSQLSSRKGKEKVTHVT 3068

Query: 3090 DRNEIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAESKSHLSDQP 3149
            D  +++        + + + L LLN+P  L+S +HL  ++ LL+V++  A S+       
Sbjct: 3069 DSRDLE--------IPLVVFLKLLNRPQLLQSTSHLGLVMGLLQVVVYTAASR------- 3128

Query: 3150 TQSTVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGEFDIHSILAN 3209
             +        PE  L +  V G+ +S  +     S+L     ++ +      +I++I   
Sbjct: 3129 IEGWSPSSGVPE-KLENKPV-GEEASSETRKDAESELVGEADLSVARRKNCAEIYNIFLQ 3188

Query: 3210 LPEAELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAM 3269
            LP+++L  LC LL  EGLSD  Y+L GEV+KKL  +   H + F  ELSE    L+ S +
Sbjct: 3189 LPQSDLCNLCILLGYEGLSDKIYSLAGEVLKKLAAVDVAHRKFFTKELSELASSLSSSTV 3248

Query: 3270 DELHMFGEAVK-ALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKEHASALTLV 3329
             EL       K + S  S  GA+ILRVLQ LSSL + + E    +    E+E  +   ++
Sbjct: 3249 RELATLSSKQKMSRSTGSMAGASILRVLQVLSSLTSPIDESNVGTERETEQEEQN---IM 3308

Query: 3330 CDINAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTP--PLPAGSQNILP 3389
              +N ALEPLW ELS CI   E   D +     S   P     G++P   L  G+Q +LP
Sbjct: 3309 QRLNVALEPLWHELSQCISMTELQLDHT--AAASNINPGDHVLGISPTSSLSPGTQRLLP 3368

Query: 3390 YIESFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRF 3449
             IE+FFV+CEK+    P   Q+    T  EV+++ A     +T+V +QK  +  + F +F
Sbjct: 3369 LIEAFFVLCEKIQ--TPSMLQQDTNVTAGEVKESSAHGSSSKTSVDSQKKTDGSVTFSKF 3428

Query: 3450 SEKHRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHH--SPLRI 3509
            +EKHR+LLN+FIRQNP LLEKS S MLK PR IDFDNK+++FRSRI+HQHD H   PLRI
Sbjct: 3429 AEKHRRLLNSFIRQNPSLLEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRI 3488

Query: 3510 SVRRAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGAL 3569
            SVRRAY+LEDSYNQLRMRS QDLKGRL V FQGEEGIDAGGL+REWYQ+LSRVIFDKGAL
Sbjct: 3489 SVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGAL 3548

Query: 3570 LFTTVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVK 3629
            LFTTVGND+TFQPNPNS YQTEHLSYFKFVGR+V KAL+DGQLLDV+FTRSFYKHILGVK
Sbjct: 3549 LFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVK 3608

Query: 3630 VTYHDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGR 3664
            VTYHDIEA+DPDYYKNLKW+LEND+SD+L LTFS+DADEEK ILYEKTEVTDYEL PGGR
Sbjct: 3609 VTYHDIEAVDPDYYKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGR 3668

BLAST of CmoCh12G001650 vs. TAIR 10
Match: AT1G55860.1 (ubiquitin-protein ligase 1 )

HSP 1 Score: 2210.6 bits (5727), Expect = 0.0e+00
Identity = 1523/3836 (39.70%), Postives = 2173/3836 (56.65%), Query Frame = 0

Query: 33   PPKIKAFIDKVIQCPLHDIAIHLSGFRWEYNKGNYHHWRPLFLHFDTYFKTYLSCRKDL- 92
            P KIK+FI+ V   PL  I   L+ FRWE++KG++HHW  LF +FDT+F+ ++  RKDL 
Sbjct: 261  PSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDTFFEKHVQVRKDLH 320

Query: 93   LSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLE-YFNLLLASTDPEILIAAL 152
            + +   E D PFPK A+LQ+LRV+++VLENC NK      E + +LLLASTD +++ A L
Sbjct: 321  IEENFEESDPPFPKDAVLQVLRVIRVVLENCTNKHFYSSYEQHLSLLLASTDADVVEACL 380

Query: 153  ETLSALVKINPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCVVADEITREEG 212
            +TL+A +K    K   R       S+NS L SLAQGWG KEEGLGL SC           
Sbjct: 381  QTLAAFLKRQIGKYSIRD-----ASLNSKLFSLAQGWGGKEEGLGLTSCA---------- 440

Query: 213  RCLFPHDVENDTNNAQYRIGSSLYFELHGCGAKSSDESSSSSSSSNSRVIHIPDLHLKKE 272
                    EN  +    ++G +L+FE +      SDE S S      +VIH+PD+ +  E
Sbjct: 441  -------AENSCDQVSLQLGRTLHFEFY-----PSDE-SPSELPGGLQVIHVPDVSICAE 500

Query: 273  DDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLAFIVLVQSGDSH 332
             DL +L + +  ++VPP LRF+LLTR+R+ARAF S   R+ ++ I L AF+VLVQ+    
Sbjct: 501  SDLELLNKLVIDHNVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDT 560

Query: 333  DELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSHERRILSGSSIS 392
            + +V+FF  EPE+ NEL+ +V  E+TVP  IR L + +L A  S   + +  +L+  +  
Sbjct: 561  ENVVSFFNGEPEFVNELVTLVSYEDTVPEKIRILCLLSLVA-LSQDRTRQPTVLTAVT-- 620

Query: 393  FAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLHMVSSSASGNNIRSSGMVP 452
             +GG+R +L  ++QKAI S+       SLAF EALL    + +VSSS+  + +R +G++P
Sbjct: 621  -SGGHRGLLSGLMQKAIDSVVCITSKWSLAFAEALLSLVTV-LVSSSSGCSAMREAGLIP 680

Query: 453  TFLPLLEDSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKELGGVEILVERLQTEVHRVIG 512
            T +PL++D+DP HLHLV  AV  L+  MD+S+ A +LF++LGG++  + RL+ EV R   
Sbjct: 681  TLVPLIKDTDPQHLHLVSAAVHILEAFMDYSNPAAALFRDLGGLDDTIFRLKLEVSRT-- 740

Query: 513  LSGNNIDSMIIGESSRCNDDQL--------YNQKRMIKVSLKALGVATYVP------TSS 572
               +  +     +S+  + +QL        Y+++ ++K  L+A+ + TY P       S 
Sbjct: 741  -EDDVKEKNCSSDSNGPDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYGSE 800

Query: 573  TNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLLHELGLPDAFLASVAAG 632
             + LP+ L +IF     FGG ++  A T+MS++IHKDPTC+  L   GL   FL +++  
Sbjct: 801  ESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSTFLDAISDE 860

Query: 633  ILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKEKYVLVM-NEAIVPLAN 692
            ++ S +AITC+P  + A+CLN  GL+AVK+  ALR  + IFT   Y+  +  +    L++
Sbjct: 861  VICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFTSPSYLRALTGDTPGSLSS 920

Query: 693  AVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGNTA-----METDSDDK 752
             ++EL RH SSLR+ GVD+ +E++  +  +G      +S   +  T+     ME D D+K
Sbjct: 921  GLDELLRHQSSLRTYGVDMFIEILNSMLIIGSGMEATTSKSADVPTSAAPVPMEIDVDEK 980

Query: 753  DNNSIVTEEV--------INTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEALLK 812
              +  V++E          N E  +  C+ ++  L    ++N+E C +FVE  GI+A+L+
Sbjct: 981  --SLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDAVLQ 1040

Query: 813  LLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSGSF 872
            L   P +  S   ++    ++ FK F+  HSA LAR  CS LR+HLKK       + G+ 
Sbjct: 1041 LFSLPLMPLST--SLGQSFSVAFKNFSPQHSAGLARIVCSYLREHLKKTKILLVSIEGTQ 1100

Query: 873  LLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQIL 932
            LL   +    KI  SL  +E +L L++    +  A++++E      +VL+++G  ++Q +
Sbjct: 1101 LLKLESAIQTKILRSLSCLEGMLSLSNFL-LKGSASVISELSAADADVLKELGITYKQTI 1160

Query: 933  WQIALLEDVK-PELEDESTGSITELQ-NSEVHTNEIEEQRFNSFR-QFLDPLLRRRTSG- 992
            WQ+AL  D K  E +    GS   +  +S     E +E   N+   ++ +P+  R +S  
Sbjct: 1161 WQMALCNDTKEDEKKSVDRGSDNSVSASSSTAERESDEDSSNALAVRYTNPVSIRSSSSQ 1220

Query: 993  --WSVESQFFDLINLYRDL-GRAPSSLQRM--GPNGSPLLQFGANNQRRSESSDTIGASN 1052
              W  + +F  ++     + GR   ++ RM  G     L  F  +    SE    +  ++
Sbjct: 1221 SIWGGDREFLSIVRSGEGIHGRTRHAIARMRGGRTRRHLESFNFD----SEIPADLPVTS 1280

Query: 1053 RKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAVASTL 1112
                 K ++      +++  L+         L K     +RRR D  ++SS+SK + + L
Sbjct: 1281 SSHELKKKSTEVLIAEILNKLNCTLRFFFTALVKGFTSANRRRIDGASLSSASKTLGTAL 1340

Query: 1113 SSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLD-RPDSCNTVLLNCLYGH 1172
            + + L+ +NF G+  A+G E S+S KCRY GKV+D +  +  D R   C T ++N  Y H
Sbjct: 1341 AKVFLEALNFDGYGAAAGHEKSLSVKCRYLGKVVDDITFLSFDTRRRVCFTAMVNSFYVH 1400

Query: 1173 GVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHS-WIQGPLASYGRLM 1232
            G    +LTTFEATSQLL+T+   P S   +   N K  E+    HS W+   L +Y R +
Sbjct: 1401 GTFKELLTTFEATSQLLWTV---PFSIPASSTENEKPGERNIWSHSKWLVDTLQNYCRAL 1460

Query: 1233 DHLVTSPFILSSFTK-HLLAQP----LTSGDVPFPRDAETFVKVLQSMVLKAVLPVWTHP 1292
            D+ V S ++LS  ++  LL QP    L+ G  P PR+ ETFV+ LQS VL  +LP+W HP
Sbjct: 1461 DYFVNSTYLLSPTSQTQLLVQPASVGLSIGLFPVPREPETFVRNLQSQVLDVILPIWNHP 1520

Query: 1293 QFIDCSCEFITTVISIIRHIYSGV-----EVKSVSSNSSARIAGPPPNETAISTIVEMGF 1352
             F DC+  F+ +V S++ HIYSGV         V+   + R      +E+ +  IVEMGF
Sbjct: 1521 MFPDCNPNFVASVTSLVTHIYSGVVDARENRSGVTRGINQRALPLQLDESIVGMIVEMGF 1580

Query: 1353 SRGRAEEALRQVGSNSVELAMEWLFSHPEE-VQEDDELARALALSLGN-SELEMKEPVSS 1412
            SR RAE ALR+VG+NSVE+AM+WLF++PE+ VQEDDELA+ALALSLGN SE    E    
Sbjct: 1581 SRSRAEIALRRVGTNSVEMAMDWLFTNPEQPVQEDDELAQALALSLGNSSETPKLEDTEK 1640

Query: 1413 EVS-KQIEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNVMSFL 1472
             V   Q E   + P  +E+++  V+L ++ +++AFP+ DL V +C++N G++R  ++S+L
Sbjct: 1641 PVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSMAFPLMDLFVTLCNRNKGEDRPKIVSYL 1700

Query: 1473 IESVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQWDSGL 1532
            I+ +K   ++  S ++  L+ + H++AL+L++D   RE A Q+G+VTV+  +L+ ++   
Sbjct: 1701 IQQLK-LVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVTVAIGILTDFNLKS 1760

Query: 1533 SFGVKVEVPKWVTAAFLAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTID--EDKQNKLQ 1592
                ++  PK ++A  L +  +LQ + K + E  +     N GG  +  D  +D    L+
Sbjct: 1761 ESETEILAPKCISALLLVLSMMLQAQTKLSSEYVE----GNQGGSLVPSDSPQDSTAALK 1820

Query: 1593 SAL-----------------GLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVLQLCS 1652
             AL                 G S  Y+ +    + + IAC  IK+ +P   M AVLQLC+
Sbjct: 1821 DALSSDVAKGESNQALELIFGKSTGYLTMEEGHKALLIACGLIKQHVPAMIMQAVLQLCA 1880

Query: 1653 SLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDPQTLQQAMESEIR 1712
             LT+SH++A+ FLE GGL+SL +LP    FPG+D++AS I+RH++EDPQTLQ AME+EIR
Sbjct: 1881 RLTKSHALAIQFLENGGLSSLFNLPKKCCFPGYDTVASVIVRHLVEDPQTLQIAMETEIR 1940

Query: 1713 HTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIEMIGERPYVVLLKDR 1772
             TL  +  RH  GRV PR FL  +  VISRDPV+FM+A  S CQ+E  G R +V+L K++
Sbjct: 1941 QTL--SGKRH-IGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGRDFVILSKEK 2000

Query: 1773 EKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD--SRSSRVHKKSS 1832
            EK K    +                +  PLG +         +K HD   + S+ H++  
Sbjct: 2001 EKPKVSGSE------------HGFSLNEPLGIS--------ENKLHDVSGKCSKSHRRVP 2060

Query: 1833 QNFINVIELLLESVYTFIPPMK--DDVTVELTSGAQASSDMDIDVAAVKGKGKAIASLSE 1892
             NFI VI+ L++ V +F P +K  +D    L S       M++D    K KGK+     E
Sbjct: 2061 ANFIQVIDQLIDLVLSF-PRVKRQEDGETNLIS-------MEVDEPTTKVKGKSKVGEPE 2120

Query: 1893 DDANSQ----------EASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSRPVH 1952
              ++S+          E S  LA+V FILKLL++I+LMY+    V++RRDTE+   R  +
Sbjct: 2121 KASSSRVGEPEKAEIPEKSEELARVTFILKLLSDIVLMYSHGTSVILRRDTEISQLRGSN 2180

Query: 1953 QRESNGGCTGGIIHHILHGFIPLSRNSKKEKKV-DGDWKHKLATRGSQFLVASCVRSSEA 2012
              + + G  GG+I+H++H  +P+S     EK V   +WK KL+ + S FLV  C RS+E 
Sbjct: 2181 LPDDSPG-NGGLIYHVIHRLLPIS----LEKFVGPEEWKEKLSEKASWFLVVLCSRSNEG 2240

Query: 2013 RRRIFVEVGSILNQFIDSCNGSRP----PSSDLQAFVDLLNDMLAARTPT------GSYI 2072
            R+RI  E+  +L+ F      S      P   + AF +L+  +L   + +      G   
Sbjct: 2241 RKRIINELSRVLSVFASLGRSSSKSVLLPDKRVLAFANLVYSILTKNSSSSSSNFPGCGC 2300

Query: 2073 TTEASATFVDAGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTK-----EHVHFGDS 2132
            + + + + +D G ++  T IL V+DLDHPD+PK+VT ++K+LE +T+     E +     
Sbjct: 2301 SPDVAKSMMDGGTIQCLTSILHVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKSEVP 2360

Query: 2133 NAGKGDSSSKTPDHH----QPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYGG 2192
            N  K   S +  D H    +   + + +   +++ V+ +        Q+   +A +  G 
Sbjct: 2361 NEKKNRDSDERHDSHGNSTETEADELNQNNSSLQQVTDAAGNGQEQAQVSSQSAGE-RGS 2420

Query: 2193 SEAVTDDMEHDQDLHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDE 2252
            S+  T  M  D  + G      E   M    EE  G          QIE+  HV    D+
Sbjct: 2421 SQ--TQAMPQDMRIEGDETILPEPIQMDFMREEIEG---------DQIEMSFHVENRADD 2480

Query: 2253 DDDEEMSGDDGDEVDEDEDEDEDEDEEEQNDLEDDEVHHLPHPDTDHDDHEID--DDEFD 2312
            D D++M    GDE ++DE +DED       DL +D    +    TD +D E     DE++
Sbjct: 2481 DVDDDM----GDEGEDDEGDDEDA------DLVEDGAGVMSLAGTDVEDPEDTGLGDEYN 2540

Query: 2313 EVLEEDDEDDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQN---ETLHVMPVE---- 2372
            + + ++D+DD  +   + +R  E ++G+   DH ++ GR    N   + +   P E    
Sbjct: 2541 DDMVDEDDDDFHENRVIEVRWREALDGL---DHFQILGRSGGGNGFIDDITAEPFEGVNV 2600

Query: 2373 --IFGSRR----QGRTTSIYNLLGRTGDTVAPSRHPLLGGPG------------------ 2432
              +F  RR    + R  +  + L R+G  V   +HPL   P                   
Sbjct: 2601 DDLFALRRPLGFERRRQTGRSSLDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSISRH 2660

Query: 2433 ------------LHATPFRPSENNRDMVISERSLENNSSG--LDTVFRSLRSGRHGHRLN 2492
                        +  TP  P +       S R     +     D     + S R G   +
Sbjct: 2661 SEAGSYDVAQFYMFDTPVLPFDQVPVDPFSARLAGGGAPPPLTDYSVVGMDSSRRGVGDS 2720

Query: 2493 LWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTPEKLSELDANAEPDNRDGNGQIQTS-- 2552
             W +       S +  I Q +EE  +S LR   P        N   +      +IQ    
Sbjct: 2721 RWTDIGHPQPSSLSASIAQLIEEHFISNLRASAP-------VNTVVERETNTTEIQEQLH 2780

Query: 2553 ---EPSGRSETIVENSGQHG-------------NDNLPPPVAINSSDGTNSRPAA----- 2612
                PS  SET++ +  + G             N N PP V   S     +  A+     
Sbjct: 2781 PDVPPSVGSETVLGDGNEGGQQSEERELLNNNENVNNPPDVMAESFAQGQANLASPVSQD 2840

Query: 2613 -SESLQGTQVIQ----QSQPVE---MQFEHNDAAVRDV---EAVSQESGGSGATFGESLR 2672
              ESLQ  +V+Q     S P E   M+    D A  D    EAV   S   G     S++
Sbjct: 2841 TGESLQQLEVMQPLPLNSTPNEIDRMEVGEGDGAPIDQVDHEAVHLISTAQGQPDTSSIQ 2900

Query: 2673 SLDVEIGSYDGHDDGGDRQGSADRMSLGDSQAARMRRSNVSFTNSASLSGRDTSLHIVTE 2732
            ++ V   +    D   + Q S D     D                + L G +  L  +  
Sbjct: 2901 NVSVTAIAPPVDDPDSNFQPSVDVDMSSDGAEGNQS------VQPSPLDGDNNELSSMEA 2960

Query: 2733 VSENSSREADEEGPVGEQQTNSDTGLGAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNE 2792
                 + E  EEG +  +       + AIDP FL+ALPE+LRAEVL++QQ Q VQPP+ E
Sbjct: 2961 TENVRNDEQVEEGSLDGRAPE----VNAIDPTFLEALPEDLRAEVLASQQAQSVQPPTYE 3020

Query: 2793 PQNSGDIDPEFLAALPPDIRAEVLAQQQAQRLHLSQELEGQPVEMDTVSIIATFPSDLRE 2852
            P    DIDPEFLAALPPDI+ EVLAQQ+AQR  + Q+ +GQ V+MD  SIIAT P+DLRE
Sbjct: 3021 PPPVDDIDPEFLAALPPDIQTEVLAQQRAQR--MVQQSQGQAVDMDNASIIATLPADLRE 3080

Query: 2853 EVLLTSSDAILANLTPALVAEANMLRERFAHRYHNR-SLFGMHPR--NRRGEITRRVEGI 2912
            EVLLTSS+A+LA L   L+AEA MLR+R    Y  R S+FG   R  NRR  +      +
Sbjct: 3081 EVLLTSSEAVLAALPSPLLAEAQMLRDRAMSHYQARSSVFGSSHRLNNRRNGL--GYNRL 3140

Query: 2913 SGLDRTGGSISSRRSL-----GSRLIEAEGAPLVDTDALHAMIRLLRVVQPLYKGQLQRL 2972
            +G+DR  G    +R++     G ++ E EG PLV+ DAL ++IRLLR+ QPL KG LQRL
Sbjct: 3141 TGMDRGVGVTIGQRAVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRL 3200

Query: 2973 LLNLCAHNETRTSLVKILMDVLLFDRRKPANQSNTTELS----YRLYACQRNVIYSRPSF 3032
            LLNLCAH+ TR +LV++L+D++     +P  +++ +EL+     RLY CQ NV+Y R   
Sbjct: 3201 LLNLCAHSFTRANLVQLLLDMI-----RPEMETSPSELAITNPQRLYGCQSNVVYGRSQL 3260

Query: 3033 FDGAPPLVSRRVLETLTYLARNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAVDRN 3092
             +G PPLV RRVLE LTYLA NH  VA +L    +F  +L    +  +   K     D  
Sbjct: 3261 LNGLPPLVFRRVLEVLTYLATNHSAVADMLF---YFDSSLLSQLSSRKGKEKVTHVTDSR 3320

Query: 3093 EIDCRPQSGGYLSIALLLSLLNQPLYLRSIAHLEQLLNLLEVIIDNAESKSHLSDQPTQS 3152
            +++        + + + L LLN+P  L+S +HL  ++ LL+V++  A S+        + 
Sbjct: 3321 DLE--------IPLVVFLKLLNRPQLLQSTSHLGLVMGLLQVVVYTAASR-------IEG 3380

Query: 3153 TVEQPAAPEVSLSDAEVNGQSSSVSSGVGTSSKLGSYFKMAASAADGEFDIHSILANLPE 3212
                   PE  L +  V G+ +S  +     S+L     ++ +      +I++I   LP+
Sbjct: 3381 WSPSSGVPE-KLENKPV-GEEASSETRKDAESELVGEADLSVARRKNCAEIYNIFLQLPQ 3440

Query: 3213 AELRLLCSLLAREGLSDNTYALVGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAMDEL 3272
            ++L  LC LL  EGLSD  Y+L GEV+KKL  +   H + F  ELSE    L+ S + EL
Sbjct: 3441 SDLCNLCILLGYEGLSDKIYSLAGEVLKKLAAVDVAHRKFFTKELSELASSLSSSTVREL 3500

Query: 3273 HMFGEAVK-ALSISSSDGAAILRVLQALSSLVALLVEKRKDSSILPEKEHASALTLVCDI 3332
                   K + S  S  GA+ILRVLQ LSSL + + E    +    E+E  +   ++  +
Sbjct: 3501 ATLSSKQKMSRSTGSMAGASILRVLQVLSSLTSPIDESNVGTERETEQEEQN---IMQRL 3560

Query: 3333 NAALEPLWLELSTCIGKIESYSDSSPDTLVSFRTPTSKPTGVTP--PLPAGSQNILPYIE 3392
            N ALEPLW ELS CI   E   D +     S   P     G++P   L  G+Q +LP IE
Sbjct: 3561 NVALEPLWHELSQCISMTELQLDHT--AAASNINPGDHVLGISPTSSLSPGTQRLLPLIE 3620

Query: 3393 SFFVVCEKLHPAQPGSDQELNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRFSEK 3452
            +FFV+CEK+    P   Q+    T  EV+++ A     +T+V +QK  +  + F +F+EK
Sbjct: 3621 AFFVLCEKIQ--TPSMLQQDTNVTAGEVKESSAHGSSSKTSVDSQKKTDGSVTFSKFAEK 3680

Query: 3453 HRKLLNAFIRQNPGLLEKSFSPMLKVPRFIDFDNKRSHFRSRIKHQHDHH--SPLRISVR 3512
            HR+LLN+FIRQNP LLEKS S MLK PR IDFDNK+++FRSRI+HQHD H   PLRISVR
Sbjct: 3681 HRRLLNSFIRQNPSLLEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVR 3740

Query: 3513 RAYILEDSYNQLRMRSTQDLKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGALLFT 3572
            RAY+LEDSYNQLRMRS QDLKGRL V FQGEEGIDAGGL+REWYQ+LSRVIFDKGALLFT
Sbjct: 3741 RAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFT 3800

Query: 3573 TVGNDSTFQPNPNSGYQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTY 3632
            TVGND+TFQPNPNS YQTEHLSYFKFVGR+V KAL+DGQLLDV+FTRSFYKHILGVKVTY
Sbjct: 3801 TVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTY 3860

Query: 3633 HDIEAIDPDYYKNLKWMLENDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGRNTK 3664
            HDIEA+DPDYYKNLKW+LEND+SD+L LTFS+DADEEK ILYEKTEVTDYEL PGGRN +
Sbjct: 3861 HDIEAVDPDYYKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIR 3920

BLAST of CmoCh12G001650 vs. TAIR 10
Match: AT1G70320.1 (ubiquitin-protein ligase 2 )

HSP 1 Score: 2206.0 bits (5715), Expect = 0.0e+00
Identity = 1497/3817 (39.22%), Postives = 2170/3817 (56.85%), Query Frame = 0

Query: 30   SEPPPKIKAFIDKVIQCPLHDIAIHLSGFRWEYNKGNYHHWRPLFLHFDTYFKTYLSCRK 89
            SE P KI  FI+ V   PL  I   L+ FRWE++KG++HHW  LF HFDT+F+ ++  RK
Sbjct: 9    SEVPTKISLFINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDTFFEKHVKVRK 68

Query: 90   DL-LSDKILEDDSPFPKHAILQILRVMQIVLENCHNKGSLDGLE-YFNLLLASTDPEILI 149
            DL + ++  E D PFPK A+LQ+LRV+++VLENC NK      E + +LLLASTD +++ 
Sbjct: 69   DLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENCTNKQFYTSYEQHLSLLLASTDADVVE 128

Query: 150  AALETLSALVKINPSKLHGRGKLIGCGSVNSYLLSLAQGWGSKEEGLGLYSCVVADEITR 209
            A L+TL+A +K    K   R       S+N  L SLAQGWG KEEGLGL SC        
Sbjct: 129  ACLQTLAAFLKRPTGKYSIRD-----ASLNLKLFSLAQGWGGKEEGLGLTSCA------- 188

Query: 210  EEGRCLFPHDVENDTNNAQYRIGSSLYFELHGCGAKSSDESSSSSSSSNSRVIHIPDLHL 269
                       E+  +    ++G +L FE +     +SDE S S      +VIH+PD+ +
Sbjct: 189  ----------TEHSCDQLFLQLGCTLLFEFY-----ASDE-SPSELPGGLQVIHVPDVSM 248

Query: 270  KKEDDLTVLKQCIELYDVPPELRFSLLTRIRYARAFHSSNLRRLYSKICLLAFIVLVQSG 329
            + E DL +L + +  ++VPP LRF+LLTR+R+ARAF S   R+ Y+ I L AFIVLVQ+ 
Sbjct: 249  RSESDLELLNKLVIDHNVPPSLRFALLTRLRFARAFSSLATRQQYTCIRLYAFIVLVQAS 308

Query: 330  DSHDELVAFFANEPEYTNELIRIVRSEETVPGTIRTLAMRALGAQFSAYSSHERRILSGS 389
               + +V+FF  EPE+ NEL+ +V  E+TVP  IR L +++L A  S   + +  +L+  
Sbjct: 309  GDTENVVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVA-LSQDRTRQPTVLTAV 368

Query: 390  SISFAGGNRTILLNVLQKAILSLKNSNDPSSLAFVEALLQFYLLHMVSSSASGNNIRSSG 449
            +   +GG+R +L  ++QKAI S+  +    SLAF EALL    + +VSSS+  + +R +G
Sbjct: 369  T---SGGHRGLLSGLMQKAIDSVICNTSKWSLAFAEALLSLVTV-LVSSSSGCSAMREAG 428

Query: 450  MVPTFLPLLEDSDPSHLHLVCLAVKTLQKLMDFSSSAVSLFKELGGVEILVERLQTEVHR 509
            ++PT +PL++D+DP HLHLV  AV  L+  MD+S+ A +LF++LGG++  + RL+ EV R
Sbjct: 429  LIPTLVPLIKDTDPQHLHLVSTAVHILEVFMDYSNPAAALFRDLGGLDDTIFRLKQEVSR 488

Query: 510  -------VIGLSGNNIDSMIIGESSRCNDDQLYNQKRMIKVSLKALGVATYVP------T 569
                   ++  SG+N       +         Y+++ ++K  L+A+ + TY P       
Sbjct: 489  TEDDVKEIVCCSGSNGPEDDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYG 548

Query: 570  SSTNTLPDILSLIFGNVDKFGGDIYCSAVTLMSEIIHKDPTCYPLLHELGLPDAFLASVA 629
            S  + LP+ L +IF     FGG ++  A T+MS++IHKDPTC+  L   GL  AFL +++
Sbjct: 549  SEESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSAFLDAIS 608

Query: 630  AGILPSPKAITCVPNGIGAICLNARGLEAVKETLALRFLIDIFTKEKYVLVM-NEAIVPL 689
              ++ S +AITC+P  + A+CLN  GL+AVK+  ALR  + IF+   Y+  + ++    L
Sbjct: 609  DEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFSSPSYLKALTSDTPGSL 668

Query: 690  ANAVEELWRHVSSLRSSGVDIILEVIEKITSLGDKDPVDSSGKLNGNT-----AMETDSD 749
            ++ ++EL RH SSLR+ GVD+ +E++  I  +G      +S   +  T      ME D D
Sbjct: 669  SSGLDELLRHQSSLRTYGVDMFIEILNSILIIGSGMEATTSKSADVPTDAAPVPMEIDVD 728

Query: 750  DKDNNSIVTEEV--------INTEQVVQLCIFHLMVLVHRTMENSETCRIFVENSGIEAL 809
            +K  +  V++E          N E  +  C+ ++  L    ++N+E C +FVE  GI+ +
Sbjct: 729  EK--SLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDTV 788

Query: 810  LKLLLRPTIAQSANGTIALHSTMVFKGFTQHHSAPLARAFCSSLRDHLKKVLTGFDLVSG 869
            L+L   P +  S   ++    ++ FK F+  HSA LAR  CS LR+HLKK       + G
Sbjct: 789  LQLFSLPLMPLST--SLGQSFSVAFKNFSPQHSAGLARILCSYLREHLKKTNNLLVSIEG 848

Query: 870  SFLLDPRTTPDEKIFSSLFLVEFLLFLADSKDNRWVAALLTEFGNESKNVLEDIGRVHRQ 929
            + LL   +    KI  SL  +E +L L++    +  A++++E    + +VL+++G  ++Q
Sbjct: 849  TQLLKLESAVQTKILRSLSCLEGMLSLSNFL-LKGSASVISELSAANADVLKELGITYKQ 908

Query: 930  ILWQIALL----EDVKPELEDESTGSITELQNSEVHTNEIEEQRFNSFR-QFLDPLLRRR 989
             +WQ+AL     ED K  ++  S  S++   +S     E +E   N+   ++ +P+  R 
Sbjct: 909  TIWQMALCNDTKEDEKKSVDRASDNSVS--ASSSTAERESDEDSSNALAVRYTNPVSIRS 968

Query: 990  TSG---WSVESQFFDLINLYRDL-GRAPSSLQRM--GPNGSPLLQFGANNQRRSESSDTI 1049
            +S    W    +F  ++   R + G    ++ RM  G     L  F  +    SE    +
Sbjct: 969  SSSQSIWGGHREFLSVVRSGRGVHGHTRHAIARMRGGRTRRHLESFNFD----SEIPADL 1028

Query: 1050 GASNRKEYSKHRTCYASCCDLVRSLSFHTTHLIQELGKVMLLPSRRRDDVVNVSSSSKAV 1109
              ++     K ++      +++  L+         L K     +RRR D  ++SS+SK +
Sbjct: 1029 PVTSSSHELKKKSTEVLIAEILNKLNCTLRFFFTSLVKGFTSANRRRIDGPSLSSASKTL 1088

Query: 1110 ASTLSSLVLDHMNFVGHVNASGSEGSISTKCRYFGKVIDFVDGILLD-RPDSCNTVLLNC 1169
             + L+ + L+ +NF G+  A+G + S+S KCRY GKV+D +  +  D R   C T ++N 
Sbjct: 1089 GTALAKVFLEALNFQGYGAAAGPDTSLSLKCRYLGKVVDDITFLTFDTRRRVCFTAMVNS 1148

Query: 1170 LYGHGVVSSVLTTFEATSQLLFTINRAPSSPMETDDANLKQDEKTDNDHS-WIQGPLASY 1229
             Y HG    +LTTFEATSQLL+   + P S   +   N K  E+    HS W+   L +Y
Sbjct: 1149 FYVHGTFKELLTTFEATSQLLW---KVPFSIRASSTENEKSGERNLWSHSKWLVDTLQNY 1208

Query: 1230 GRLMDHLVTSPFILSSFTK-HLLAQP----LTSGDVPFPRDAETFVKVLQSMVLKAVLPV 1289
             R +D+ V S ++LS  ++  LL QP    L+ G  P PR+ ETFV+ LQS VL+ +LP+
Sbjct: 1209 CRALDYFVNSTYLLSPTSQTQLLVQPASVDLSIGLFPVPREPETFVRNLQSQVLEVILPI 1268

Query: 1290 WTHPQFIDCSCEFITTVISIIRHIYSGV----EVKSVSSNSSARIAGP-PPNETAISTIV 1349
            W HP F DC+  F+ +V S++ HIYSGV    E +S ++  + + A P  P+E  +  IV
Sbjct: 1269 WNHPMFPDCNPNFVASVTSLVTHIYSGVVDTRENRSGATQGTNQRALPLQPDEAIVGMIV 1328

Query: 1350 EMGFSRGRAEEALRQVGSNSVELAMEWLFSHPEE-VQEDDELARALALSLGN-SELEMKE 1409
            EMGFSR RAE+ALR+VG+NSVE+AM+WLF++PE+ VQEDDELA+ALALSLGN SE    E
Sbjct: 1329 EMGFSRSRAEDALRRVGTNSVEMAMDWLFTNPEDPVQEDDELAQALALSLGNSSETPKLE 1388

Query: 1410 PVSSEVS-KQIEESVQLPCTEELLSTCVRLLRAKETLAFPVRDLLVMICSQNDGQNRSNV 1469
                 V   Q E   + P  +E+++  V+L ++ +++AFP+ DL V +C++N G++R  +
Sbjct: 1389 DTEKPVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSIAFPLVDLFVTLCNRNKGEDRPKI 1448

Query: 1470 MSFLIESVKGCDKISDSGNSTTLSALFHVIALILNDDTVAREAAYQNGLVTVSSNLLSQW 1529
            + +LI+ +K   ++  S ++  L+ + H++AL+L++D   RE A Q+G+V V+  +L+ +
Sbjct: 1449 VFYLIQQLK-LVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVAVAIGILTDF 1508

Query: 1530 DSGLSFGVKVEVPKWVTAAFLAIDRLLQEEKKFNPEIADQLKRDNVGGDTLTIDE----- 1589
            +        +  PK ++A  L +  +LQ + + + E  +     N GG  +  D      
Sbjct: 1509 NLKSESETDILAPKCISALLLVLSMMLQAQTRLSSEYVE----GNQGGSLVLSDSPQDST 1568

Query: 1590 --------------DKQNKLQSALGLSPKYIDVGNQKELIEIACSCIKKRLPCETMHAVL 1649
                          +    L+S  G S  Y+ +    +++ IAC  IK+R+P   M AVL
Sbjct: 1569 AALKDALSSDVAKGESNQALESMFGKSTGYLTMEESSKVLLIACGLIKQRVPAMIMQAVL 1628

Query: 1650 QLCSSLTRSHSVAVSFLEAGGLTSLLSLPTTSLFPGFDSIASTIIRHILEDPQTLQQAME 1709
            QLC+ LT+SH++A+ FLE GGL+SL +LP    FPG+D++AS I+RH++EDPQTLQ AME
Sbjct: 1629 QLCARLTKSHALAIQFLENGGLSSLFNLPKKCFFPGYDTVASVIVRHLVEDPQTLQIAME 1688

Query: 1710 SEIRHTLITAMNRHPNGRVTPRNFLLGLNSVISRDPVIFMRATQSVCQIEMIGERPYVVL 1769
            +EIR TL  +  RH  GRV PR FL  +  VISRDPV+FM+A  S CQ+E  G   +V+L
Sbjct: 1689 TEIRQTL--SGKRH-IGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGTDFVIL 1748

Query: 1770 LKDREKDKSKEKDRDKEKSMEKEKTQNHDVKVPLGNANPATAGNVRSKFHD--SRSSRVH 1829
             K++EK K    +                +  PLG +         +K HD   + S+ H
Sbjct: 1749 TKEKEKPKVSGSE------------HGFSLNEPLGIS--------ENKLHDGSGKCSKSH 1808

Query: 1830 KKSSQNFINVIELLLESVYTFIPPMKDDVTVELTSGAQASSDMDIDVAAVKGKGKAIASL 1889
            ++   NFI VI+ L++ V +F P +K          A     MD+D    K KGK+    
Sbjct: 1809 RRVPTNFIQVIDQLIDIVLSF-PGLK-----RQEGEAANLISMDVDEPTTKVKGKSKVGE 1868

Query: 1890 SEDDANSQEASASLAKVVFILKLLTEILLMYASSVHVLIRRDTEVCCSRPVHQRESNGGC 1949
             E      E S  LA+V FILKLL++I+LMY     V++RRDTE+   R  +  + + G 
Sbjct: 1869 PEKAELGSEKSEELARVTFILKLLSDIVLMYLHGTSVILRRDTEISQLRGSNLPDDSPG- 1928

Query: 1950 TGGIIHHILHGFIPLSRNSKKEKKV-DGDWKHKLATRGSQFLVASCVRSSEARRRIFVEV 2009
             GG+I+H++H  +P+S     EK V   +WK KL+ + S FLV  C RS+E R+RI  E+
Sbjct: 1929 NGGLIYHVIHRLLPIS----LEKFVGPEEWKEKLSEKASWFLVVLCSRSNEGRKRIINEL 1988

Query: 2010 GSILNQFIDSCNGSRP----PSSDLQAFVDLLNDMLAARTPTGSY----ITTEASATFVD 2069
              +L+ F      S      P   + AF +L+  +L   + + ++     + + + + +D
Sbjct: 1989 TRVLSVFASLGRSSSQSVLLPDKRVLAFANLVYSILTKNSSSSNFPGCGCSPDVAKSMID 2048

Query: 2070 AGLVRSFTQILKVLDLDHPDSPKVVTGVIKALELVTK-----EHVHFGDSNAGKGDSSSK 2129
             G ++  T IL V+DLDHPD+PK+VT ++K+LE +T+     E +     N  K   S +
Sbjct: 2049 GGTIQCLTSILNVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKSEVPNEQKNTDSDE 2108

Query: 2130 TPDHHQPGGENIGETPRAMETVSQSNHESIPAEQIEPYNANQIYG-GSEAVTDDMEHDQD 2189
              D H        +      +  Q   +++   Q +P  ++Q  G    ++T  M  +  
Sbjct: 2109 RHDSHGTSTSTEVDELNQNNSSLQQVTDAVDNGQEQPQVSSQSEGERGSSLTQAMLQEMR 2168

Query: 2190 LHGVFGANAEDECMHVTPEEARGLENGIDTVDIQIEIQPHVPENLDEDDDEEMSGDDGDE 2249
            + G      E   M    EE  G     D +++   ++    +++D+D D+E   D+GD+
Sbjct: 2169 IEGDETILPEPIQMDFFREEIEG-----DQIEMSFHVEDRADDDVDDDMDDEGEDDEGDD 2228

Query: 2250 VDEDEDED------------EDEDE----EEQND--LEDDEVHHLPHPDTDHDDHEIDDD 2309
             D D  ED            ED ++    +E ND  +++DE     + D   D+ E D+D
Sbjct: 2229 EDADSVEDGAGVMSIAGTDVEDPEDTGLGDEYNDDMVDEDEEDEDEYNDDMVDEDEDDED 2288

Query: 2310 EFDEVLEEDDEDDEDDEDGVLLRLEEGINGINVFDHVEVFGRDTSQN---ETLHVMPVE- 2369
            E+++ + ++DEDD  +   + +R  E ++G+   DH ++ GR    N   + +   P E 
Sbjct: 2289 EYNDDMVDEDEDDFHETRVIEVRWREALDGL---DHFQIVGRSGGGNGFIDDITAEPFEG 2348

Query: 2370 -----IFGSRR----QGRTTSIYNLLGRTGDTVAPSRHPLLGGPG--------------- 2429
                 +F  RR    + R  +  +   R+G  V   +HPL   P                
Sbjct: 2349 VNVDDLFALRRSLGFERRRQTGRSSFDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSI 2408

Query: 2430 ---------------LHATPFRPSENNRDMVISERSLENNSSG--LDTVFRSLRSGRHGH 2489
                           +  +P  P +       S+R     +     D     + S R G 
Sbjct: 2409 SRHSEAGSYDVAQFYMFDSPVLPFDQVPVDPFSDRLGGGGAPPPLTDYSVVGMDSSRRGV 2468

Query: 2490 RLNLWANDIQHNGGSNNGVIPQGLEELLVSQLRRPTP-EKLSELDANAEPDNRDGNGQIQ 2549
              + W +       S +  I Q +EE  ++ LR   P + + E + N           + 
Sbjct: 2469 GDSRWTDVGHPQPSSLSASIAQLIEEHFITNLRASAPVDTVVERETNTTEVQEQQQPDV- 2528

Query: 2550 TSEPSGRSETIV-------ENSGQH---GNDNLPPPVAINSSDGTNSRPAASESLQGTQV 2609
               PS  SET++       E S +H    N+ +  P+ +NS+     R    E       
Sbjct: 2529 --PPSVGSETVLGDGNEGGEQSEEHELLNNNEVMHPLPLNSTPNEIDRMEVGEG------ 2588

Query: 2610 IQQSQPVEMQFEHNDAAVRDVEAVSQESGGSGATFGESLRSLDVEIGSYDGHDDGGDRQG 2669
                 P+E           D EAV   S   G +    ++++ V        D   + Q 
Sbjct: 2589 --GGAPIEQV---------DREAVHLISSAQGQSDTSGIQNVSVTAIPPPVDDPDSNFQP 2648

Query: 2670 SADRMSLGDSQAARMRRSNVSFTNSASLSGRDTSLHIVTEVSENSSREADEEGPVGEQQT 2729
            S D     D                + L G +  L  +    +  + E  +EG +  +  
Sbjct: 2649 SVDVDMSSDGAEGNQS------VQPSPLDGDNNELSSMEATQDVRNDEQVDEGSLDGRAP 2708

Query: 2730 NSDTGLGAIDPAFLDALPEELRAEVLSTQQGQVVQPPSNEPQNSGDIDPEFLAALPPDIR 2789
                 + AIDP FL+ALPE+LRAEVL++QQ Q VQPP+ EP +  DIDPEFLAALPP+I+
Sbjct: 2709 E----VNAIDPTFLEALPEDLRAEVLASQQAQSVQPPTYEPPSVDDIDPEFLAALPPEIQ 2768

Query: 2790 AEVLAQQQAQRLHLSQELEGQPVEMDTVSIIATFPSDLREEVLLTSSDAILANLTPALVA 2849
             EVLAQQ+AQR  + Q+ +GQPV+MD  SIIAT P+DLREEVLLTSS+A+LA L P L+A
Sbjct: 2769 REVLAQQRAQR--MLQQSQGQPVDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLA 2828

Query: 2850 EANMLRERFAHRYHNRS-LFGMHPR--NRRGEITRRVEGISGLDRTGGSISSRRSL---- 2909
            EA MLR+R    Y  RS +FG   R  NRR  +  R   ++G++R  G    +R +    
Sbjct: 2829 EAQMLRDRAMRHYQARSRVFGSSHRLNNRRNGLGYR---LTGMERGVGVTIGQRDVSSSA 2888

Query: 2910 -GSRLIEAEGAPLVDTDALHAMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMD 2969
             G ++ E EG PLV+ DAL ++IRLLR+ QPL KG LQRLLLNLCAH+ TR +LV++L+D
Sbjct: 2889 DGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRLLLNLCAHSFTRANLVQLLLD 2948

Query: 2970 VLLFDRRKPANQSNTTELS----YRLYACQRNVIYSRPSFFDGAPPLVSRRVLETLTYLA 3029
            ++     +P  ++  +EL+     RLY CQ NV+Y R    +G PPLV RRVLE LTYLA
Sbjct: 2949 MI-----RPEMETLPSELALTNPQRLYGCQLNVVYGRSQLLNGLPPLVFRRVLEVLTYLA 3008

Query: 3030 RNHPHVAKILLQCKFFRPTLQESENVNQDCGKAVMAVDRNEIDCRPQSGGYLSIALLLSL 3089
             NH  VA +L    +F  +L    +  +   K     D  +++        + + + L L
Sbjct: 3009 TNHSAVADMLF---YFDSSLLSQLSSRKGKEKVTHETDSRDLE--------IPLVVFLKL 3068

Query: 3090 LNQPLYLRSIAHLEQLLNLLEVIIDNAESKSHLSDQPTQSTVEQPAAPEVSLSDAEVNGQ 3149
            LN+P  L+S +HL  ++ LL+V++  A S+        +        PE  L +  V  +
Sbjct: 3069 LNRPQLLQSTSHLALVMGLLQVVVYTAASR-------IEGWSPSSGVPE-KLENKPVGEE 3128

Query: 3150 SSSVSSGVGTSSKLGSYFKMAASAADGEFDIHSILANLPEAELRLLCSLLAREGLSDNTY 3209
            +SS +     S       +++ +      ++++I   LP+++L  LC LL  EGLSD  Y
Sbjct: 3129 ASSETQKDAES-------ELSVARRKNCAELYNIFLQLPQSDLCNLCMLLGYEGLSDKIY 3188

Query: 3210 ALVGEVMKKLVDIAPIHCRLFITELSESVEKLTRSAMDELHMFGEAVK-ALSISSSDGAA 3269
            +L GEV+KKL  +   H + F  ELSE    L+ S +  L       K + +  S  GA+
Sbjct: 3189 SLAGEVLKKLAAVDVTHRKFFTKELSELASGLSSSTVRVLATLSTTQKMSQNTCSMAGAS 3248

Query: 3270 ILRVLQALSSLVALLVEKRKDSSILPEKE-HASALTLVCDINAALEPLWLELSTCIGKIE 3329
            ILRVLQ LSSL + +     DS++  +KE       ++  +  ALEPLW EL  CI   E
Sbjct: 3249 ILRVLQVLSSLTSTI----DDSNVGTDKETDQEEQNIMQGLKVALEPLWQELGQCISMTE 3308

Query: 3330 SYSDSSPDTLVSFRTPTSKPTGVTP--PLPAGSQNILPYIESFFVVCEKLHPAQPGSDQE 3389
               D +  T  S   P     G++P   L  G+Q++LP IE+FFV+CEK+    P   Q+
Sbjct: 3309 LQLDHTAAT--SNVNPGDHVLGISPTSSLSPGTQSLLPLIEAFFVLCEKIQ--TPSMLQQ 3368

Query: 3390 LNIATVSEVEDAGASAVLQRTTVPTQKVDEKHIAFIRFSEKHRKLLNAFIRQNPGLLEKS 3449
                T  EV+++       +T V +QK  +  + F +F EKHR+LLN+F+RQNP LLEKS
Sbjct: 3369 DATVTAGEVKESSTHGSSSKTIVDSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPSLLEKS 3428

Query: 3450 FSPMLKVPRFIDFDNKRSHFRSRIKHQHDHH--SPLRISVRRAYILEDSYNQLRMRSTQD 3509
            FS MLK PR IDFDNK+++FRSRI+HQHD H   PLRISVRRAY+LEDSYNQLRMRS QD
Sbjct: 3429 FSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQD 3488

Query: 3510 LKGRLTVHFQGEEGIDAGGLSREWYQVLSRVIFDKGALLFTTVGNDSTFQPNPNSGYQTE 3569
            LKGRL V FQGEEGIDAGGL+REWYQ+LSRVIFDKGALLFTTVGND+TFQPNPNS YQTE
Sbjct: 3489 LKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTE 3548

Query: 3570 HLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTYHDIEAIDPDYYKNLKWMLE 3629
            HLSYFKFVGR+V KAL+DGQLLDV+FTRSFYKHILGVKVTYHDIEA+DPDYYKNLKW+LE
Sbjct: 3549 HLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLE 3608

Query: 3630 NDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGRNTKVTEENKYQYVDLVVEHQLT 3664
            ND+SD+L LTFS+DADEEK ILYEKTEVTDYEL PGGRN +VTEE K++YVDLV +H LT
Sbjct: 3609 NDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILT 3657

BLAST of CmoCh12G001650 vs. TAIR 10
Match: AT3G17205.1 (ubiquitin protein ligase 6 )

HSP 1 Score: 238.4 bits (607), Expect = 9.0e-62
Identity = 151/407 (37.10%), Postives = 232/407 (57.00%), Query Frame = 0

Query: 3272 MLKVPRFIDFDNKRSHFRSRIKHQHDHHSPL------RISVRRAYILEDSYNQLRMRSTQ 3331
            +++ P  I F ++   F +++      H         R  +RR +ILED+YNQ+   S  
Sbjct: 631  LMQAPFLIPFTSRVKIFTTQLATARQSHGSQGIFARNRFRIRRDHILEDAYNQMSALSED 690

Query: 3332 DLKGRLTVHFQG-----EEGIDAGGLSREWYQVLSRVIFDKGALLFTTVGNDSTFQPNPN 3391
            DL+  + V F       E GID GG+ +++ + ++R  FD    LF     D    PNP 
Sbjct: 691  DLRSSIRVTFVNELGVEEAGIDGGGIFKDFMEKITRAAFDVQYGLFKETA-DHMLYPNPG 750

Query: 3392 SG-YQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTY-HDIEAIDPDYY 3451
            SG    +HL +F F+G ++ KA+++G L+D+ F  +F+   L  K  Y +D+ ++DP+ Y
Sbjct: 751  SGMIHEQHLQFFHFLGSLLAKAMFEGILVDIPFA-TFFLSKLKQKYNYLNDLPSLDPELY 810

Query: 3452 KNLKWM--LENDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGRNTKVTEENKYQY 3511
            ++L ++   + DISD L L F +       +  E  E T+ EL+PGG++ +VT EN   +
Sbjct: 811  RHLIFLKRYKGDISD-LELYFVI-------LNNEYGERTEEELLPGGQDMRVTNENVITF 870

Query: 3512 VDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFNDKELELLICGLPD-IDLDDMRAN 3571
            + LV  H+L   IR Q + FL GF ++IP+E I +FN+ EL++LI G  D +D+DD+R N
Sbjct: 871  IHLVSNHRLNFQIRQQSSHFLRGFQQLIPKEWIDMFNEHELQVLISGSVDSLDIDDLRNN 930

Query: 3572 TEYS-GYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTSKVPLEGFSALQGISGSQKFQ 3631
            T Y+ GY A   VI  FWEV+++FS E++ + L+FVTG S+ PL GF  L+     Q+  
Sbjct: 931  TNYAGGYHAGHYVIDMFWEVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQRAA 990

Query: 3632 IHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAIHEANEGF 3662
               +  S D LP++ TC N L LP Y SK+ LE +L+ AI  A  GF
Sbjct: 991  GSASNESVDRLPTSATCMNLLKLPPYQSKELLETKLMYAI-SAEAGF 1026

BLAST of CmoCh12G001650 vs. TAIR 10
Match: AT3G17205.2 (ubiquitin protein ligase 6 )

HSP 1 Score: 238.4 bits (607), Expect = 9.0e-62
Identity = 151/407 (37.10%), Postives = 232/407 (57.00%), Query Frame = 0

Query: 3272 MLKVPRFIDFDNKRSHFRSRIKHQHDHHSPL------RISVRRAYILEDSYNQLRMRSTQ 3331
            +++ P  I F ++   F +++      H         R  +RR +ILED+YNQ+   S  
Sbjct: 617  LMQAPFLIPFTSRVKIFTTQLATARQSHGSQGIFARNRFRIRRDHILEDAYNQMSALSED 676

Query: 3332 DLKGRLTVHFQG-----EEGIDAGGLSREWYQVLSRVIFDKGALLFTTVGNDSTFQPNPN 3391
            DL+  + V F       E GID GG+ +++ + ++R  FD    LF     D    PNP 
Sbjct: 677  DLRSSIRVTFVNELGVEEAGIDGGGIFKDFMEKITRAAFDVQYGLFKETA-DHMLYPNPG 736

Query: 3392 SG-YQTEHLSYFKFVGRVVGKALYDGQLLDVHFTRSFYKHILGVKVTY-HDIEAIDPDYY 3451
            SG    +HL +F F+G ++ KA+++G L+D+ F  +F+   L  K  Y +D+ ++DP+ Y
Sbjct: 737  SGMIHEQHLQFFHFLGSLLAKAMFEGILVDIPFA-TFFLSKLKQKYNYLNDLPSLDPELY 796

Query: 3452 KNLKWM--LENDISDVLGLTFSVDADEEKLILYEKTEVTDYELIPGGRNTKVTEENKYQY 3511
            ++L ++   + DISD L L F +       +  E  E T+ EL+PGG++ +VT EN   +
Sbjct: 797  RHLIFLKRYKGDISD-LELYFVI-------LNNEYGERTEEELLPGGQDMRVTNENVITF 856

Query: 3512 VDLVVEHQLTTAIRPQINAFLDGFHEIIPRELISIFNDKELELLICGLPD-IDLDDMRAN 3571
            + LV  H+L   IR Q + FL GF ++IP+E I +FN+ EL++LI G  D +D+DD+R N
Sbjct: 857  IHLVSNHRLNFQIRQQSSHFLRGFQQLIPKEWIDMFNEHELQVLISGSVDSLDIDDLRNN 916

Query: 3572 TEYS-GYSAASPVIQWFWEVVQAFSKEDKARLLQFVTGTSKVPLEGFSALQGISGSQKFQ 3631
            T Y+ GY A   VI  FWEV+++FS E++ + L+FVTG S+ PL GF  L+     Q+  
Sbjct: 917  TNYAGGYHAGHYVIDMFWEVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQRAA 976

Query: 3632 IHKAYGSPDHLPSAHTCFNQLDLPEYPSKQHLEERLILAIHEANEGF 3662
               +  S D LP++ TC N L LP Y SK+ LE +L+ AI  A  GF
Sbjct: 977  GSASNESVDRLPTSATCMNLLKLPPYQSKELLETKLMYAI-SAEAGF 1012