CmoCh12G001380 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G001380
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Serine hydroxymethyltransferase
Location	Cmo_Chr12: 868285 .. 873048 (-)
RNA-Seq Expression	CmoCh12G001380
Synteny	CmoCh12G001380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGTTTCTAAGAAAAGTCGCCGTCGGCGTTACGAAACCCAACGTTTCTCCGGTGAATGGGCGGTTCCTCCTCCGTCACATGGTGGTTTTCTCTCTCCTCGCCGCTAATTCATAATATCTATGATTTTTCTGTTTTGATGCCCAAATTTTCCGTCGGTTTTCTTTGTCCGCAGTCGTCTGTTTGCAGGTCCACGGCGGAGGAAAGGGAAGCTCGAGCTACGGTGAGCACTCCATTTCTATGATTTTCTTTTTTTAAAAAAAAGAAAATTATATTTATTTTATGTCAAAATTGTTGATATTAATTTAATTAATTATAAATATCATAGATAAATATTTTCAATTTTTCAATGTGTGTTTTTCTTTTTTATTTGATAAAAATCAAAATTTAAAATTAAACTAAAAAGAAAAAAAATATTATGTCTATATTTTTTCATATGCATAAATAATTTAACATTCCCATTTGAGGAGACTTTCATCCAACAACACCTCCCCTCGAACAAAGTAAGACTCTTATTTATCTGAGGCTTTGACTCTTCTTTTGGAGTCCTTTGTTCCACATTTGAGAATTCTATTGATATGACTCAGTTTAGGGCATGGCTCTAATATCAAATTAGAGGAACACGATTCTCTATAATAGGATGGACAATATCAAGTTAATCAAGTTAGAAGAATACGATTCTCCATAATAGTATGATATTGTCAGGCATAAGCTCTCGTGGGTTTGATTTAGAATTTTTTTTTTCTTCCACTTTTGCAGTGGATTAAGCAGCTGAACTCCCCCATGGAGGTCATTGCTCCCGATGTGGCAGACATCATTGAGCTTGAGAAAGCTCGTCAATTCCAGGTTTTTTTTTTCTTTTTTTGTTTTATTTTTTAATCGAAATGGTCGGTTGAATTGAGTTGAGTTTAGCTTCAAGTCGTTTTTTAATTTGAAGGGTCTTGAGCTTATACCTTCCGAGAATTTCTGCCCAAGAGCAGTAATGGAAGCAGTTGGTTCGATATTGACCAATTCATATAGTGAAGGATATCCGGGTGCCAGATACTATGGAGGAACTGAGTCTGTCAATCAATCTATTTCAATTGTACACTACAATTTCCTTTGTTCGATTCCTGTTTGAATTAATTACAAACCCAAAATGGATTTAGGTACGCTGATATCATCGAGAACTTGTGCAAGAAACGTGTGTTTGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGGTATCATAATTGTTCATATCTTATTATTATGATCTTTTCTTAGTAGTGAGCTTAATGGGTATCTTTATGTCCTAAGTTATGTTAGGAAACACGGATCTCCACAAATGGTATGATATTGAACACTTTGAGCATGAGCTCTCGTGGCTTTGCTTTGGGCTTCCCCAAAATATCTCATACCAATGGAGATAGTATTCCTCAACTTATAAACTCATGATCTTCCACTAAATTAACCAACGTGGGACTCACTCTCAATAATCCTCAACAAGTTATGCTAGAATTTCCCCGAGTTTCTTGTGATTCAGCTGCACATTTGCAAATAGTTTCAGAATTTTCTCAATATGAAGTTGATTAACTGAACATGTTCTTTTGTTTGTAGTTAACGTGCAGACTTTCACTGGATCTATAGCTAACTTTCTGACATACAGTGCATTATTAAAACCTCACGAGAGGATAATGTCTCTTGATTTACCACATGGTGGACACCTTTCGCACGGTTATCAGGTAGGTCCTTAATTTTTAAATGTAGTACATGTTAGTTTTTGGTTTAGTTACAAATTTGTTCGGGAAAAGTAGGCAAAGTTTAAACTGAGTTACTATGATCTTAAAAATTATGAACGTTTCCTTTTAGATTTTCTTCGTAAGAAACCGAACTATCATCGAGAACATGAGCTTGTAAGTGATTTCATATTTGTATATGTTTCTTCGAACTTTTGCAGACTGCTACCAAGAAAGTTTCTCTTGTTTCAATGTTCTTCGAGACAATGCCATATAGGTTGGATGAGAGAACTGGTTACATTGACTATGATGGGGTTAGCAAATGCTTCTGTACCGTTTGAATGAATTGTATACTGATATCCTATTGATTTTTCTTGAAACTGCAATCCAAATAATCAAAACTTATATGCAGATGGAGAGAAGTGCATCTTTGTTTCGACCAAAACTCATTGTTGCTGGTGCAACTGCCTACGCTCGTCTCTATGATTATGCACGAATTCGAAAGGTTTCTTTTTCATTTGCTTGGCTATAAATTACATTTTGGAATATCTCCACGAGGGTGTTACTTTTACATTTGTAGCATAACATTTGATTTCGTCGTGACAGATCTGTGACAAATATAAGGCAATCATGATGGCTGATATGGCACACCTTGCTGGTTTAGTTGCAGCAGACGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACGACCACAAATAAGTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGTAAGTGAGACGTAAAAATGTAGCCAAAAAAACAATTTGAGCATAAGCTCTCGTGGCCTTGCTTTTGGCTTCCCAAAAGCCCACATAAATCCATGTTCTTCTACTAAATTAATTAAGGTGGGATTTACTCCTAATAATCCTCAACAATCCTCCCCTCGAACAAAGTAGTACATTATAAGCCTCCCCTTAATCGAGGCTCGACTCCTTTCTCTAGGGCTCTCAGACAAAGTACACCCTTTGTTCGACACTTTAGTCATTTTTTACTACACCTTCAAGGCTTACAATTCTTTGTTTAACGAGGATTCTATTGACATAGCTAAGTTCAGGACATGACTTTGATATCATGTTAGAAATCACGACTCTCCACAATTGTATGATATTGTCCACTTGAGTATAAGCTCTCGTGACTTTGCTTTGGGCTTCCCCAAAAGACCTCCTACCAGTGGAGATAGTATTCCTCACTTATAAACCCATGATCTTTCACTAAATTAACCAATGTGAGACTCATTTCACTAAATTAACCAATGTGAGACTCACTTCCAATAATTCTCAACAAGGTGCTCTACGACTATGAAGACAAAATCAATGGCGCAGTCTTCCCTGGTTTTCAAGGTTCCCCACACAACCATAAAATTGCTGGTCTGGCGATTGCTTTAAAGCAGGTTGTTTATTTAATTCAAAACATCCTCGGTAACAGAATTAAATTGTTTTCATTCATGTTATTTCTGTTCTTTACCATATTACAATAGGCCACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGGTATACAGACTAGTGCATGTTTTTCTCACTGCTTTGAATTCTTTGCTTAAATCAGTGTAGTTTACTGTGTCAAATTCATTGTTTATGCAGTGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGATAATCATTTACTCCTCGTGAATTTGAAAAATAAGGTAAAGGGTGACTTCTGGTTCTTCGTTATAACTATTATACTTCTCATCCACGTATTTTTCCTTATGTAGTGATGGAGATTACAATTTTATTGACGGTTTAACTAATAGTAAAAATCTATTTGGAATAACTTTTCAAAGCGTTAGAAAGTCAGTCTAATAAAGCATTTAAAACTTTAAAAATTTAACCGATGCTTTTAGATTAAAAAAAATTGACGAATGAGTCAAGCATCTCTTATTTATTACTGAAAATTCAATAACTTATTGATTACAAAACTAAAAGTTGAAGGACGGATTAGATACTTTTTAAAATTCATGACTTTGAAAAACAAAATTGGAACTTCAAAGGATTTATCATACAAGTTTAAAGACCAAAGAGACACAAATTTAAAAGTTTGGGGACTAAATTTGTAATTTAATCTATTTTCAACCTAATACAGGGTATTGATGGCTCGAGAGTGGAAAAGGTTTTGGATTGGGTGCATATTGCAGCTAACAAGAACACAGTTCCAGGGGATGTGTCTGCCGTTTCTCCGAGTGGCATTCGTATGGGTATGACTTTCGAGTTCATGATATAAAATAATATGTCATGTAATAACATATCAAATACTGAATAAGAGTATAGACTATGGTAATACTATAAATTTTGTGTATAATAGGAACCCCTGCTCTTACTTCCCGAGGCTTCCTCGAAGCAGATTTCGTGAAAGTAGCTGAATTCTTTGATGAAGCTACGAAACTAGCCTTGAAGATTAAGGCTGCTGAACCTAAAGGTCAGATTCTCGATCGTAACAAGTATCAAATCTGCATTAAAATATACTGAACTCTCACATCTCACATTATTGTCCATTTGTTGGCTCGCTATCTTTTAGAATCTTTTGACATACTCAATCGTGAATGCAGATGCTAAGTTAAAGGATTTTGAGGCAACCGTGCAATCAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACGGTAGGTTTTGAGAAAGAGACCATGAAATATAGAGACTGAAGAACAGAGTTTTATAGGCACCTGAGTCGGGTTAGACAGTCTGCTAATAAATGGCAGCGGTTCGTTAAAACCCAGTACTTGAAAAGTCATTTTAAATTTGTGTTAGTTGCTCAACCGTTTATATGCATAAATAAATGTCTTTTGTGAACCGTCTTTTTTGACTTTACACTCTTGATCTTAAAAATTTAACATAACCAAAATCTCG

mRNA sequence

ATGGCAATGTTTCTAAGAAAAGTCGCCGTCGGCGTTACGAAACCCAACGTTTCTCCGGTGAATGGGCGGTTCCTCCTCCGTCACATGTCGTCTGTTTGCAGGTCCACGGCGGAGGAAAGGGAAGCTCGAGCTACGTGGATTAAGCAGCTGAACTCCCCCATGGAGGTCATTGCTCCCGATGTGGCAGACATCATTGAGCTTGAGAAAGCTCGTCAATTCCAGGGTCTTGAGCTTATACCTTCCGAGAATTTCTGCCCAAGAGCAGTAATGGAAGCAGTTGGTTCGATATTGACCAATTCATATAGTGAAGGATATCCGGGTGCCAGATACTATGGAGGAACTGAGTACGCTGATATCATCGAGAACTTGTGCAAGAAACGTGTGTTTGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGTTAACGTGCAGACTTTCACTGGATCTATAGCTAACTTTCTGACATACAGTGCATTATTAAAACCTCACGAGAGGATAATGTCTCTTGATTTACCACATGGTGGACACCTTTCGCACGGTTATCAGACTGCTACCAAGAAAGTTTCTCTTGTTTCAATGTTCTTCGAGACAATGCCATATAGGTTGGATGAGAGAACTGGTTACATTGACTATGATGGGATGGAGAGAAGTGCATCTTTGTTTCGACCAAAACTCATTGTTGCTGGTGCAACTGCCTACGCTCGTCTCTATGATTATGCACGAATTCGAAAGATCTGTGACAAATATAAGGCAATCATGATGGCTGATATGGCACACCTTGCTGGTTTAGTTGCAGCAGACGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACGACCACAAATAAGTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGTGCTCTACGACTATGAAGACAAAATCAATGGCGCAGTCTTCCCTGGTTTTCAAGGTTCCCCACACAACCATAAAATTGCTGGTCTGGCGATTGCTTTAAAGCAGGCCACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGTGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGATAATCATTTACTCCTCGTGAATTTGAAAAATAAGGGTATTGATGGCTCGAGAGTGGAAAAGGTTTTGGATTGGGTGCATATTGCAGCTAACAAGAACACAGTTCCAGGGGATGTGTCTGCCGTTTCTCCGAGTGGCATTCGTATGGGAACCCCTGCTCTTACTTCCCGAGGCTTCCTCGAAGCAGATTTCGTGAAAGTAGCTGAATTCTTTGATGAAGCTACGAAACTAGCCTTGAAGATTAAGGCTGCTGAACCTAAAGATGCTAAGTTAAAGGATTTTGAGGCAACCGTGCAATCAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACGGTAGGTTTTGAGAAAGAGACCATGAAATATAGAGACTGAAGAACAGAGTTTTATAGGCACCTGAGTCGGGTTAGACAGTCTGCTAATAAATGGCAGCGGTTCGTTAAAACCCAGTACTTGAAAAGTCATTTTAAATTTGTGTTAGTTGCTCAACCGTTTATATGCATAAATAAATGTCTTTTGTGAACCGTCTTTTTTGACTTTACACTCTTGATCTTAAAAATTTAACATAACCAAAATCTCG

Coding sequence (CDS)

ATGGCAATGTTTCTAAGAAAAGTCGCCGTCGGCGTTACGAAACCCAACGTTTCTCCGGTGAATGGGCGGTTCCTCCTCCGTCACATGTCGTCTGTTTGCAGGTCCACGGCGGAGGAAAGGGAAGCTCGAGCTACGTGGATTAAGCAGCTGAACTCCCCCATGGAGGTCATTGCTCCCGATGTGGCAGACATCATTGAGCTTGAGAAAGCTCGTCAATTCCAGGGTCTTGAGCTTATACCTTCCGAGAATTTCTGCCCAAGAGCAGTAATGGAAGCAGTTGGTTCGATATTGACCAATTCATATAGTGAAGGATATCCGGGTGCCAGATACTATGGAGGAACTGAGTACGCTGATATCATCGAGAACTTGTGCAAGAAACGTGTGTTTGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGTTAACGTGCAGACTTTCACTGGATCTATAGCTAACTTTCTGACATACAGTGCATTATTAAAACCTCACGAGAGGATAATGTCTCTTGATTTACCACATGGTGGACACCTTTCGCACGGTTATCAGACTGCTACCAAGAAAGTTTCTCTTGTTTCAATGTTCTTCGAGACAATGCCATATAGGTTGGATGAGAGAACTGGTTACATTGACTATGATGGGATGGAGAGAAGTGCATCTTTGTTTCGACCAAAACTCATTGTTGCTGGTGCAACTGCCTACGCTCGTCTCTATGATTATGCACGAATTCGAAAGATCTGTGACAAATATAAGGCAATCATGATGGCTGATATGGCACACCTTGCTGGTTTAGTTGCAGCAGACGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACGACCACAAATAAGTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGTGCTCTACGACTATGAAGACAAAATCAATGGCGCAGTCTTCCCTGGTTTTCAAGGTTCCCCACACAACCATAAAATTGCTGGTCTGGCGATTGCTTTAAAGCAGGCCACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGTGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGATAATCATTTACTCCTCGTGAATTTGAAAAATAAGGGTATTGATGGCTCGAGAGTGGAAAAGGTTTTGGATTGGGTGCATATTGCAGCTAACAAGAACACAGTTCCAGGGGATGTGTCTGCCGTTTCTCCGAGTGGCATTCGTATGGGAACCCCTGCTCTTACTTCCCGAGGCTTCCTCGAAGCAGATTTCGTGAAAGTAGCTGAATTCTTTGATGAAGCTACGAAACTAGCCTTGAAGATTAAGGCTGCTGAACCTAAAGATGCTAAGTTAAAGGATTTTGAGGCAACCGTGCAATCAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACGGTAGGTTTTGAGAAAGAGACCATGAAATATAGAGACTGA

Protein sequence

MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPDVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADIIENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHGYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD

Homology

[Close]

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 6.4e-221
Identity = 378/518 (72.97%), Postives = 447/518 (86.29%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEERE-ARATWIKQLNSPMEVIAP 60
           MA+ LR+++  V KP  S  NG  L  +MSS+      ++E +   W KQLN+P+EV+ P
Sbjct: 3   MAIALRRLSATVDKPVKSLYNGGSLY-YMSSLPNEAVYDKEKSGVAWPKQLNAPLEVVDP 62

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           ++ADIIE EKARQ++GLELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 63  EIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAFRLDPAKWGVNVQ  +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 123 AETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 182

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA+LFRPKLIVAGA+AYARLYDY
Sbjct: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDY 242

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
            RIRK+C+K KAI++ADMAH++GLVAA VIPSPF+YAD+VTTTT+KSL GPRGA+IFYRK
Sbjct: 243 DRIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRK 302

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKE+NK+G+EV YDYEDKIN AVFPG QG PHNH I GLA+ALKQATTPEY+AYQ+QVL
Sbjct: 303 GVKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVL 362

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           +NSS+FAQ L EKGYELVSGGTDNHL+LVN+KNKGIDGSRVEKVL+ VHIAANKNTVPGD
Sbjct: 363 SNSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGD 422

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGFLE DFVKVA+FFD A K+A+K+K AE +  KLKDF AT+
Sbjct: 423 VSAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVK-AETQGTKLKDFVATL 482

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           +S+   +SEI+KLR +VEE++KQFPT+GFEKETMKY++
Sbjct: 483 ESSAPIKSEIAKLRHDVEEYAKQFPTIGFEKETMKYKN 518

[Close]

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot
Match: Q94C74 (Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM2 PE=1 SV=2)

HSP 1 Score: 765.0 bits (1974), Expect = 5.5e-220
Identity = 375/518 (72.39%), Postives = 446/518 (86.10%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRST-AEEREARATWIKQLNSPMEVIAP 60
           MA+ LR+++  V KP     +    LR MSS+  +  AE  ++R++WIKQLN+ ++ I P
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VADIIELEKARQ++G ELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAF+LDP+KWGVNVQ+ +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA LFRPKLIVAGA+AYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA VIPSPFEYAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           G+KEINK+G+EV+YDYED+IN AVFPG QG PHNH I GLA+ALKQA TPEYKAYQ QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
            N S+FA+ L+ KGY+LVSGGTDNHL+LVNLKNKGIDGSRVEKVL+ VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGF+E DF KVAE+FD A K+ALKIK AE +  KLKDF AT+
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK-AESQGTKLKDFVATM 480

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           QSN++ QSE+SKLR  VEE++KQFPT+GFEKETM+Y++
Sbjct: 481 QSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.2e-219
Identity = 376/516 (72.87%), Postives = 442/516 (85.66%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEERE-ARATWIKQLNSPMEVIAP 60
           MAM LRK++  V K +  P+     L + SS+      ++E  R TW KQLNSP+EVI P
Sbjct: 3   MAMALRKLSSSVNKSS-RPLFSASSLYYKSSLPDEAVYDKENPRVTWPKQLNSPLEVIDP 62

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           ++ADIIELEKARQ++GLELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 63  EIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAFRLDPAKWGVNVQ  +GS +NF  Y+ALLKPH+RIM+LDLPHGGHLSH
Sbjct: 123 AETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSH 182

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA+LFRPKLIVAGA+AYARLYDY
Sbjct: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDY 242

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+CDK KA+++ADMAH++GLVAA VIPSPF+YAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 243 ARIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 302

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           G+KE+NK+G+EV YDYEDKIN AVFPG QG PHNH I GLA+ALKQATTPEY+AYQ+QVL
Sbjct: 303 GLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVL 362

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           +NSS+FA+ L EKGY+LVSGGT+NHL+LVNLKNKGIDGSRVEKVL+ VHIAANKNTVPGD
Sbjct: 363 SNSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 422

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGF+E DFVKVAE+FD A  LALK+K AE K  KLKDF   +
Sbjct: 423 VSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVK-AESKGTKLKDFVEAL 482

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKY 516
           Q++   QSEISKL+ +VEEF+KQFPT+GFEK TMKY
Sbjct: 483 QTSSYVQSEISKLKHDVEEFAKQFPTIGFEKATMKY 516

[Close]

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.3e-218
Identity = 374/518 (72.20%), Postives = 448/518 (86.49%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEERE-ARATWIKQLNSPMEVIAP 60
           MAM LR+++  + KP + P+  R    +MSS+     +E+E +R TW KQLN+P+E + P
Sbjct: 3   MAMALRRLSSSIDKP-IRPLI-RSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDP 62

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           ++ADIIE EKARQ++GLELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 63  EIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAFRLDP KWGVNVQ  +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 123 AETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSH 182

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD ME+SA+LFRPKLIVAGA+AYARLYDY
Sbjct: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDY 242

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA+VIPSPF+YAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 243 ARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 302

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKEINK+G+EVLYD+EDKIN AVFPG QG PHNH I GLA+ALKQATT EYKAYQ+QVL
Sbjct: 303 GVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVL 362

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           +NS++FAQ L+E+GYELVSGGTDNHL+LVNLK KGIDGSRVEKVL+ VHIA+NKNTVPGD
Sbjct: 363 SNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGD 422

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGF+E DF KVAE+FD+A  +ALK+K +E +  KLKDF + +
Sbjct: 423 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK-SEAQGTKLKDFVSAM 482

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           +S+   QSEI+KLR EVEEF+KQFPT+GFEKETMKY++
Sbjct: 483 ESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot
Match: P49357 (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 1.4e-215
Identity = 368/517 (71.18%), Postives = 434/517 (83.95%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPD 60
           MA+ LR+++    KP     NG  L    S    +  E+     TW KQLN+P+EV+ P+
Sbjct: 3   MALALRRLSSSADKPLQRLFNGGHLYSMSSLPSEAVYEKERPGVTWPKQLNAPLEVVDPE 62

Query: 61  VADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADII 120
           +ADIIELEKARQ++GLELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+ 
Sbjct: 63  IADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHG 180
           E LC+KR  EAFRLDPAKWGVNVQ  +GS ANF  Y+ALLK H+RIM+LDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLSHG 182

Query: 181 YQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYA 240
           YQT TKK+S VS+FFETMPYRL+E TGYIDYD +E+SA+LFRPKLIVAGA+AYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKG 300
           RIRK+CDK KAIM+ADMAH++GLVAA VIPSPF+YAD+VTTTT+KSL GPRGA+IF+RKG
Sbjct: 243 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLN 360
           +KE+NK+G+EV YDYEDKIN AVFPG QG PHNH I GLA+ALKQATT EYKAYQ+QV++
Sbjct: 303 LKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQVMS 362

Query: 361 NSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDV 420
           NS++FA+ L++ GYELVSGGT+NHL+LVNLKNKGIDGS+VEKVL+ VHIAANKNTVPGDV
Sbjct: 363 NSAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPGDV 422

Query: 421 SAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQ 480
           SA+ P GIRMGTPALTSRGF+E DF KVA FFD A KLA+KIK  E K  KLKDF  T  
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDLAVKLAVKIK-GEAKGTKLKDF-VTAM 482

Query: 481 SNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
            +   QSEISKLR +VEE++KQFPT+GFEKETMKY++
Sbjct: 483 ESSAIQSEISKLRHDVEEYAKQFPTIGFEKETMKYKN 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL
Match: A0A6J1GH35 (Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 PE=3 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 8.8e-298
Identity = 517/517 (100.00%), Postives = 517/517 (100.00%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPD 60
           MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPD
Sbjct: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPD 60

Query: 61  VADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADII 120
           VADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADII
Sbjct: 61  VADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADII 120

Query: 121 ENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHG 180
           ENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHG
Sbjct: 121 ENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHG 180

Query: 181 YQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYA 240
           YQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYA
Sbjct: 181 YQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYA 240

Query: 241 RIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKG 300
           RIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKG
Sbjct: 241 RIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKG 300

Query: 301 VKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLN 360
           VKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLN
Sbjct: 301 VKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLN 360

Query: 361 NSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDV 420
           NSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDV
Sbjct: 361 NSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDV 420

Query: 421 SAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQ 480
           SAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQ
Sbjct: 421 SAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQ 480

Query: 481 SNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           SNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD
Sbjct: 481 SNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL
Match: A0A6J1GI73 (Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.2e-291
Identity = 508/516 (98.45%), Postives = 510/516 (98.84%), Query Frame = 0


Query: 2   AMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEEREARATWIKQLNSPMEVIAPDV 61
           AMFLR+VAVG TK NV+P NGRFLLRHMSS   STAEEREARATWIKQLNSPMEVIAPDV
Sbjct: 34  AMFLRRVAVGATKLNVAPANGRFLLRHMSSRYGSTAEEREARATWIKQLNSPMEVIAPDV 93

Query: 62  ADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADIIE 121
           ADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADIIE
Sbjct: 94  ADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADIIE 153

Query: 122 NLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHGY 181
           NLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHGY
Sbjct: 154 NLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHGY 213

Query: 182 QTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYAR 241
           QTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYAR
Sbjct: 214 QTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYAR 273

Query: 242 IRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKGV 301
           IRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKGV
Sbjct: 274 IRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKGV 333

Query: 302 KEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLNN 361
           KEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLNN
Sbjct: 334 KEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLNN 393

Query: 362 SSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDVS 421
           SSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDVS
Sbjct: 394 SSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDVS 453

Query: 422 AVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQS 481
           AVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQS
Sbjct: 454 AVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQS 513

Query: 482 NKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           NKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD
Sbjct: 514 NKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 549

[Close]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL
Match: A0A6J1KHZ9 (Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.1e-275
Identity = 477/517 (92.26%), Postives = 498/517 (96.32%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRH-MSSVCRSTAEEREARATWIKQLNSPMEVIAP 60
           MAM LR+VAVG+TKPNVSPVNGRFLLR+  SS   STAEEREARATWIK LN P+EV+AP
Sbjct: 1   MAMLLRRVAVGITKPNVSPVNGRFLLRNTASSTGGSTAEEREARATWIKPLNLPIEVVAP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VA+IIELEKARQFQGLELIPSENFC RAVMEAVGSI+TNSYSEGYPGARYYGG EYAD+
Sbjct: 61  EVAEIIELEKARQFQGLELIPSENFCARAVMEAVGSIMTNSYSEGYPGARYYGGIEYADM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
           IENLCK+R  EAFRLDPAKWGVNVQTFTGSIANFLTY+ALLKPHERIMSLDLPHGGHLSH
Sbjct: 121 IENLCKERALEAFRLDPAKWGVNVQTFTGSIANFLTYTALLKPHERIMSLDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQTATKK+SLVS+FFETMPYRLDE TGYIDYDGMERSASLFRPKLIVAGATAY+RLYDY
Sbjct: 181 GYQTATKKISLVSVFFETMPYRLDESTGYIDYDGMERSASLFRPKLIVAGATAYSRLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRKICDK+KAIMMADMAHLAGLVAADVIPS FEYADIVTTTTNKSLGGPRGALIFYRK
Sbjct: 241 ARIRKICDKHKAIMMADMAHLAGLVAADVIPSRFEYADIVTTTTNKSLGGPRGALIFYRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKEINKKGEEVLYDY+DKING VFPGFQGSPHNHKIAG  IALKQATTPEYKAYQQQVL
Sbjct: 301 GVKEINKKGEEVLYDYKDKINGTVFPGFQGSPHNHKIAGPVIALKQATTPEYKAYQQQVL 360

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           NNSSRFAQCLIEKGYELVSGGTDNHL+LVNLKN+GIDGSRVEKVLDWVHIAANKNTVPGD
Sbjct: 361 NNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNEGIDGSRVEKVLDWVHIAANKNTVPGD 420

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+SPSGIRMGTPALTSRGFLEADFVKVAEF+DEATKLALKIKAAEPK AKLKDFEAT+
Sbjct: 421 VSAISPSGIRMGTPALTSRGFLEADFVKVAEFYDEATKLALKIKAAEPKGAKLKDFEATM 480

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR 517
           Q NKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR
Sbjct: 481 QLNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL
Match: A0A6J1KJZ9 (Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.7e-272
Identity = 473/517 (91.49%), Postives = 496/517 (95.94%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRH-MSSVCRSTAEEREARATWIKQLNSPMEVIAP 60
           MAM LR+VAVG+TKPNVSPVNGRFLLR+  SS   STAEEREARATWIK LN P+EV+AP
Sbjct: 1   MAMLLRRVAVGITKPNVSPVNGRFLLRNTASSTGGSTAEEREARATWIKPLNLPIEVVAP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VA+IIELEKARQFQGLELIPSENFC RAVMEAVGSI+TNSYSEGYPGARYYGG EYAD+
Sbjct: 61  EVAEIIELEKARQFQGLELIPSENFCARAVMEAVGSIMTNSYSEGYPGARYYGGIEYADM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
           IENLCK+R  EAFRLDPAKWGVNVQTFTGSIANFLTY+ALLKPHERIMSLDLPHGGHLSH
Sbjct: 121 IENLCKERALEAFRLDPAKWGVNVQTFTGSIANFLTYTALLKPHERIMSLDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQTATKK+SLVS+FFETMPYRLDE TGYIDYDGMERSASLFRPKLIVAGATAY+RLYDY
Sbjct: 181 GYQTATKKISLVSVFFETMPYRLDESTGYIDYDGMERSASLFRPKLIVAGATAYSRLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRKICDK+KAIM+ADMAHLAGLVAADVIPSPFEYADIVTTTTNKSL GPRGALIF+RK
Sbjct: 241 ARIRKICDKHKAIMLADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLRGPRGALIFFRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKEINKKGEEVLYDYEDKIN AVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL
Sbjct: 301 GVKEINKKGEEVLYDYEDKINSAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           NNSS+FAQCLIEKGYELVSGGTDNHL+L+NLK KGIDGSR+EKVLDWVHIAANKNTVPGD
Sbjct: 361 NNSSKFAQCLIEKGYELVSGGTDNHLVLINLKIKGIDGSRLEKVLDWVHIAANKNTVPGD 420

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGFLEADFVKVAEFFDEA KLALKIKAAEPK AKLKDFEAT+
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDEALKLALKIKAAEPKGAKLKDFEATM 480

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR 517
           Q NKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR
Sbjct: 481 QLNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYR 517

[Close]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL
Match: A0A6J1GH68 (Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454161 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 3.2e-271
Identity = 471/518 (90.93%), Postives = 493/518 (95.17%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRH-MSSVCRSTAEEREARATWIKQLNSPMEVIAP 60
           MAM LR+VAVG+TKPNVSPVNGRFLLR+  SS   STAEEREARATWIK LN P+EV+AP
Sbjct: 1   MAMLLRRVAVGITKPNVSPVNGRFLLRNTASSAGGSTAEEREARATWIKPLNLPIEVVAP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VA+IIELEKARQFQGLELIPSENFC RAVMEAVGSI+TNSYSEGYPGARYYGG EYADI
Sbjct: 61  EVAEIIELEKARQFQGLELIPSENFCARAVMEAVGSIMTNSYSEGYPGARYYGGIEYADI 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
           IENLCK+R  EAFRLDPAKWGVNVQTFTGSIANFLTY+ALLKPHERIMSLDLPHGGHLSH
Sbjct: 121 IENLCKERALEAFRLDPAKWGVNVQTFTGSIANFLTYTALLKPHERIMSLDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQTATKK+SLVS+FFETMPYRLDE TGYIDYDGM+RSASLFRPKLIVAGATAY+RLYDY
Sbjct: 181 GYQTATKKISLVSVFFETMPYRLDESTGYIDYDGMDRSASLFRPKLIVAGATAYSRLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRKICDK+KAIM+ADMAHLAGLVAADV PSPFEYADIVTTTTNKSL GPRGALIFYRK
Sbjct: 241 ARIRKICDKHKAIMLADMAHLAGLVAADVNPSPFEYADIVTTTTNKSLRGPRGALIFYRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKEINKKGEEV+YDYEDKIN AVFPGFQGSPHNHKIAGL IALKQATTPEYKAYQQQVL
Sbjct: 301 GVKEINKKGEEVVYDYEDKINSAVFPGFQGSPHNHKIAGLVIALKQATTPEYKAYQQQVL 360

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           NNSS+FAQCLIEKGYELVSGGTDNHL+L+NLKNKGIDGSRVEKVLDWVHIAANKNTVPGD
Sbjct: 361 NNSSKFAQCLIEKGYELVSGGTDNHLVLINLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGFLEADFVKVAEFFDEA KLALKIKAAEPK  KLKDFEATV
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDEALKLALKIKAAEPKGTKLKDFEATV 480

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           QSNKEFQ EISKLR EVE+FSKQF TVGFEKETMKYRD
Sbjct: 481 QSNKEFQYEISKLRHEVEKFSKQFATVGFEKETMKYRD 518

[Close]

BLAST of CmoCh12G001380 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 765.0 bits (1974), Expect = 3.9e-221
Identity = 375/518 (72.39%), Postives = 446/518 (86.10%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRST-AEEREARATWIKQLNSPMEVIAP 60
           MA+ LR+++  V KP     +    LR MSS+  +  AE  ++R++WIKQLN+ ++ I P
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VADIIELEKARQ++G ELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAF+LDP+KWGVNVQ+ +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA LFRPKLIVAGA+AYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA VIPSPFEYAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           G+KEINK+G+EV+YDYED+IN AVFPG QG PHNH I GLA+ALKQA TPEYKAYQ QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
            N S+FA+ L+ KGY+LVSGGTDNHL+LVNLKNKGIDGSRVEKVL+ VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGF+E DF KVAE+FD A K+ALKIK AE +  KLKDF AT+
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK-AESQGTKLKDFVATM 480

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           QSN++ QSE+SKLR  VEE++KQFPT+GFEKETM+Y++
Sbjct: 481 QSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 517

[Close]

BLAST of CmoCh12G001380 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 760.4 bits (1962), Expect = 9.6e-220
Identity = 374/518 (72.20%), Postives = 448/518 (86.49%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRSTAEERE-ARATWIKQLNSPMEVIAP 60
           MAM LR+++  + KP + P+  R    +MSS+     +E+E +R TW KQLN+P+E + P
Sbjct: 3   MAMALRRLSSSIDKP-IRPLI-RSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDP 62

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           ++ADIIE EKARQ++GLELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 63  EIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAFRLDP KWGVNVQ  +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 123 AETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSH 182

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD ME+SA+LFRPKLIVAGA+AYARLYDY
Sbjct: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDY 242

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA+VIPSPF+YAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 243 ARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 302

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           GVKEINK+G+EVLYD+EDKIN AVFPG QG PHNH I GLA+ALKQATT EYKAYQ+QVL
Sbjct: 303 GVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVL 362

Query: 361 NNSSRFAQCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGD 420
           +NS++FAQ L+E+GYELVSGGTDNHL+LVNLK KGIDGSRVEKVL+ VHIA+NKNTVPGD
Sbjct: 363 SNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGD 422

Query: 421 VSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATV 480
           VSA+ P GIRMGTPALTSRGF+E DF KVAE+FD+A  +ALK+K +E +  KLKDF + +
Sbjct: 423 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK-SEAQGTKLKDFVSAM 482

Query: 481 QSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
           +S+   QSEI+KLR EVEEF+KQFPT+GFEKETMKY++
Sbjct: 483 ESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

[Close]

BLAST of CmoCh12G001380 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 755.7 bits (1950), Expect = 2.4e-218
Identity = 376/534 (70.41%), Postives = 446/534 (83.52%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRST-AEEREARATWIKQLNSPMEVIAP 60
           MA+ LR+++  V KP     +    LR MSS+  +  AE  ++R++WIKQLN+ ++ I P
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VADIIELEKARQ++G ELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAF+LDP+KWGVNVQ+ +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA LFRPKLIVAGA+AYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA VIPSPFEYAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           G+KEINK+G+EV+YDYED+IN AVFPG QG PHNH I GLA+ALKQA TPEYKAYQ QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 NNSSRFA----------------QCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKV 420
            N S+FA                Q L+ KGY+LVSGGTDNHL+LVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIK 480
           L+ VHIAANKNTVPGDVSA+ P GIRMGTPALTSRGF+E DF KVAE+FD A K+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 AAEPKDAKLKDFEATVQSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
            AE +  KLKDF AT+QSN++ QSE+SKLR  VEE++KQFPT+GFEKETM+Y++
Sbjct: 481 -AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 533

[Close]

BLAST of CmoCh12G001380 vs. TAIR 10
Match: AT5G26780.2 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 755.7 bits (1950), Expect = 2.4e-218
Identity = 376/534 (70.41%), Postives = 446/534 (83.52%), Query Frame = 0


Query: 1   MAMFLRKVAVGVTKPNVSPVNGRFLLRHMSSVCRST-AEEREARATWIKQLNSPMEVIAP 60
           MA+ LR+++  V KP     +    LR MSS+  +  AE  ++R++WIKQLN+ ++ I P
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  DVADIIELEKARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADI 120
           +VADIIELEKARQ++G ELIPSENF   +VM+AVGS++TN YSEGYPGARYYGG EY D+
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 IENLCKKRVFEAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSH 180
            E LC+KR  EAF+LDP+KWGVNVQ+ +GS ANF  Y+ALLKPHERIM+LDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTATKKVSLVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDY 240
           GYQT TKK+S VS+FFETMPYRLDE TGYIDYD +E+SA LFRPKLIVAGA+AYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKICDKYKAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRK 300
           ARIRK+C+K KA+M+ADMAH++GLVAA VIPSPFEYAD+VTTTT+KSL GPRGA+IF+RK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGEEVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVL 360
           G+KEINK+G+EV+YDYED+IN AVFPG QG PHNH I GLA+ALKQA TPEYKAYQ QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 NNSSRFA----------------QCLIEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKV 420
            N S+FA                Q L+ KGY+LVSGGTDNHL+LVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEFFDEATKLALKIK 480
           L+ VHIAANKNTVPGDVSA+ P GIRMGTPALTSRGF+E DF KVAE+FD A K+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 AAEPKDAKLKDFEATVQSNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRD 518
            AE +  KLKDF AT+QSN++ QSE+SKLR  VEE++KQFPT+GFEKETM+Y++
Sbjct: 481 -AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 533

[Close]

BLAST of CmoCh12G001380 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 522.3 bits (1344), Expect = 4.4e-148
Identity = 270/499 (54.11%), Postives = 348/499 (69.74%), Query Frame = 0


Query: 14  KPNVS-PVNGRFLLRHMSSVCRSTA---EEREARATWIKQLNSPMEVIAPDVADIIELEK 73
           K N+S P    FL R++ S  R+++      E  +  I   +  +  + P+V  II  EK
Sbjct: 39  KFNISKPFRSSFLKRNLVSEMRASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEK 98

Query: 74  ARQFQGLELIPSENFCPRAVMEAVGSILTNSYSEGYPGARYYGGTEYADIIENLCKKRVF 133
            RQF+ LELI SENF  RAVMEAVGS LTN YSEG PG RYYGG EY D +E LC+ R  
Sbjct: 99  DRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRAL 158

Query: 134 EAFRLDPAKWGVNVQTFTGSIANFLTYSALLKPHERIMSLDLPHGGHLSHGYQTATKKVS 193
            AFRLD  KWGVNVQ  +GS ANF  Y+A+L PH+RIM LDLPHGGHLSHG+ TA ++VS
Sbjct: 159 AAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVS 218

Query: 194 LVSMFFETMPYRLDERTGYIDYDGMERSASLFRPKLIVAGATAYARLYDYARIRKICDKY 253
             S++FE+MPYRLDE TG +DYD +E++A+LFRPKLI+AGA+AY+R +DY R+RKI D  
Sbjct: 219 GTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSV 278

Query: 254 KAIMMADMAHLAGLVAADVIPSPFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGE 313
            A +M DMAH++GLVAA V+  PFEY DIVTTTT+KSL GPRG +IF+RK    IN    
Sbjct: 279 GAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRK--DPING--- 338

Query: 314 EVLYDYEDKINGAVFPGFQGSPHNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCL 373
               D E  +N AVFPG QG PHNH I GLA+ LK A +PE+KAYQ++V++N    A  L
Sbjct: 339 ---VDLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQKRVVSNCRALANRL 398

Query: 374 IEKGYELVSGGTDNHLLLVNLKNKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIR 433
           +E G++LVSGG+DNHL+LV+L+  G+DG+RVEK+LD   I  NKN+VPGD SA+ P GIR
Sbjct: 399 VELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSVPGDKSALVPGGIR 458

Query: 434 MGTPALTSRGFLEADFVKVAEFFDEATKLALKIKAAEPKDAKLKDFEATVQSNK-EFQSE 493
           +G+PA+T+RG  E DFV VA+F  E  ++ ++ K A P  +KL+DF   V S +   +  
Sbjct: 459 IGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAP-GSKLQDFNKFVTSPEFPLKER 518

Query: 494 ISKLRREVEEFSKQFPTVG 508
           +  L+  VE F+ +FP  G
Sbjct: 519 VKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:

BLAST of CmoCh12G001380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50433	6.4e-221	72.97	Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2...	[more]
Q94C74	5.5e-220	72.39	Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
P34899	1.2e-219	72.87	Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=...	[more]
Q9SZJ5	1.3e-218	72.20	Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
P49357	1.4e-215	71.18	Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE...	[more]

BLAST of CmoCh12G001380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GH35	8.8e-298	100.00	Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 P...	[more]
A0A6J1GI73	1.2e-291	98.45	Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 P...	[more]
A0A6J1KHZ9	8.1e-275	92.26	Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=...	[more]
A0A6J1KJZ9	1.7e-272	91.49	Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=...	[more]
A0A6J1GH68	3.2e-271	90.93	Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454161 P...	[more]

BLAST of CmoCh12G001380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26780.1	3.9e-221	72.39	serine hydroxymethyltransferase 2	[more]
AT4G37930.1	9.6e-220	72.20	serine transhydroxymethyltransferase 1	[more]
AT5G26780.3	2.4e-218	70.41	serine hydroxymethyltransferase 2	[more]
AT5G26780.2	2.4e-218	70.41	serine hydroxymethyltransferase 2	[more]
AT4G32520.1	4.4e-148	54.11	serine hydroxymethyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 482..502
None	No IPR available	PANTHER	PTHR11680:SF49	SERINE HYDROXYMETHYLTRANSFERASE	coord: 7..516
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 83..356 e-value: 1.2E-199 score: 665.2
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 59..504 e-value: 1.2E-199 score: 665.2
IPR001085	Serine hydroxymethyltransferase	PIRSF	PIRSF000412	SHMT	coord: 35..512 e-value: 2.8E-161 score: 534.7
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 7..516
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 53..507 score: 41.011208
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 59..475 e-value: 0.0 score: 578.002
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 58..451 e-value: 2.1E-193 score: 642.4
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 47..508

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G001380.1	CmoCh12G001380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0032259	methylation
biological_process	GO:0035999	tetrahydrofolate interconversion
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity