CmoCh11G019260 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G019260
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	SPARK domain-containing protein
Location	Cmo_Chr11: 13352087 .. 13354003 (-)
RNA-Seq Expression	CmoCh11G019260
Synteny	CmoCh11G019260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGCTTTGCTTTGCTTTCTTTCATATCACTATCTCATCTCATTTTCTCCCCACCATTCAAATAGATAAAGAAAACAAATCTTTTGTACCATTCTCTTCCCCTTTCCTTTCCTTTTCTCTCTTCCTCTCCACCAAACCAATCCTCAACACTCCCACTACCAATTTCCCTCTCCTTTTACAAACAACACATGAACTTAGTGCGAAATAATTGAGGCATTATATTGCAATATATAACCAACATTCCTTCTAGTGATGCCATTTCATTTTTGTTTGAGATTTCTCCTCTTCTTCCTTCCACTTTTCTCTTCAAAACCCTTCATTTTTTTGTGGGTTTTGTTCAACATTTGAGAATGTTTTTGAATCCTCTCCAAAAGCTTGAAAAAAACCCACTTTTAAAACCCTAATCTTTACACATTTCATCACTCTTTTCCCATTCTAATCATATTTTCTCCATTACCAAACCTCTAATTAGCAAAAACAAGCATGCCCATTTCTTCATTTCCCCCAAAAATGCTTCATTTCGCCCAAAAATGTCTAATTTGCTTCGTTTTCTTAAGCATTGCTACAAATTTTCATTCAACTTCAGCTGGTTTTCTCATCGAACCGGTTTCCGGCCAAAGCCAGCCACTAAATCCCGGCGAATACTCATCCTCCAACACGGTTCCGGCCTTTCCGGTCCAAACCCAGATGCAAATTTGCCATCTTGATCTCTCCGACGAGCTTTTCGGCGGCGTTAGGGAAGCTTGCGGTCGAGACTTGGACCGGAGTCGGTGCTGTCCTGTTCTAGCCGCGTGGCTTTTTGCCGCTCATGCACGCGCCGCCCTGAAGATTACCACTCCGGCGCCGTCGGACCTTCCGATGATGCCGGATGACTCACAGAAATGCGTGAACTCGCTGCAGGCGTCGCTGTTGAGTAGAAATATAAAGATTCCACAGCCGAATGCGAGCTGCGACGCCGTTTTGTGCTTTTGTGGGATTCGGCTTCATCAAATTAGCTCGTTGTCTTGCCCCGCTGCCTTTAATGTCTCCGGCGACGGCGTTCGCGACGGCTACCGGAACGCCACCCCGACGGCCGCCGTCCGTAATCTTGAAAAGAATTGCCGGAACTCATCTTATTCCGGTTGCACGAAATGCCTTGGCGCCCTTCAAAAGGTAATATTAAATTAAAATTATATAACAATTTGTGTTTAATTTAATTAAAATGTTTCATAATTTGTAACAGTTTGGTCCTCGGAATTTCTTATTATTTTTATTTCCCCCCATTATTTTTTCCTATTTGCAAATCAGCCCCTAAGGTGTGTTTGGACGCGAGTATATAATGTTCTTTGTCGGGATTGATAAATCCAGGTTACGGCAACGAAGAAGAACGCCACCAGCGACAGAGTGAGTAAAATGTTCAACAGAGACTGCCAGCTCATGGGGCTGACGTGGCTCCTCGCCCGTAACAAAACGGCGTATATCCCCACCGTTTCGGCCGTACTACGAGCCATCATGTACAGTGCCCACCCTCCTCACGAGTCTACGTGTAGTCCGGACCAAGAAAACATGCCGCTGGCCGTCGATTCGTTACAGATGGAAAAAGCGCAGTCGTCATCGCCACCGTCCATTTCTCTGGTTCCGATTATACCCCTATTGGTTATTGGTTATTGGCTTGCTTGGTAGTGGGCGTAGGGGTAATTATGGGTTGGGATCCGTTTGTTTTGTAATTTTTGGAGCCTGTCGGGTTCACGTGAAGCTAGTGGACAAAAATAAGGGATGATGGAGGGGCTTTTTTGTCTTTTCGCTACACTGTAAATGTCAAATCTGGGTGCTTTTTTTTACTTTTTTTACTCTCTTCCCATTCGCTTTCTTGTGACAAATTTTTCCTTGGGGCTTTCAATTAAACCAGAAAGTAAAAAACTTGCGGTTCTTAAATCA

mRNA sequence

TTTGCTTTGCTTTGCTTTCTTTCATATCACTATCTCATCTCATTTTCTCCCCACCATTCAAATAGATAAAGAAAACAAATCTTTTGTACCATTCTCTTCCCCTTTCCTTTCCTTTTCTCTCTTCCTCTCCACCAAACCAATCCTCAACACTCCCACTACCAATTTCCCTCTCCTTTTACAAACAACACATGAACTTAGTGCGAAATAATTGAGGCATTATATTGCAATATATAACCAACATTCCTTCTAGTGATGCCATTTCATTTTTGTTTGAGATTTCTCCTCTTCTTCCTTCCACTTTTCTCTTCAAAACCCTTCATTTTTTTGTGGGTTTTGTTCAACATTTGAGAATGTTTTTGAATCCTCTCCAAAAGCTTGAAAAAAACCCACTTTTAAAACCCTAATCTTTACACATTTCATCACTCTTTTCCCATTCTAATCATATTTTCTCCATTACCAAACCTCTAATTAGCAAAAACAAGCATGCCCATTTCTTCATTTCCCCCAAAAATGCTTCATTTCGCCCAAAAATGTCTAATTTGCTTCGTTTTCTTAAGCATTGCTACAAATTTTCATTCAACTTCAGCTGGTTTTCTCATCGAACCGGTTTCCGGCCAAAGCCAGCCACTAAATCCCGGCGAATACTCATCCTCCAACACGGTTCCGGCCTTTCCGGTCCAAACCCAGATGCAAATTTGCCATCTTGATCTCTCCGACGAGCTTTTCGGCGGCGTTAGGGAAGCTTGCGGTCGAGACTTGGACCGGAGTCGGTGCTGTCCTGTTCTAGCCGCGTGGCTTTTTGCCGCTCATGCACGCGCCGCCCTGAAGATTACCACTCCGGCGCCGTCGGACCTTCCGATGATGCCGGATGACTCACAGAAATGCGTGAACTCGCTGCAGGCGTCGCTGTTGAGTAGAAATATAAAGATTCCACAGCCGAATGCGAGCTGCGACGCCGTTTTGTGCTTTTGTGGGATTCGGCTTCATCAAATTAGCTCGTTGTCTTGCCCCGCTGCCTTTAATGTCTCCGGCGACGGCGTTCGCGACGGCTACCGGAACGCCACCCCGACGGCCGCCGTCCGTAATCTTGAAAAGAATTGCCGGAACTCATCTTATTCCGGTTGCACGAAATGCCTTGGCGCCCTTCAAAAGGTTACGGCAACGAAGAAGAACGCCACCAGCGACAGAGTGAGTAAAATGTTCAACAGAGACTGCCAGCTCATGGGGCTGACGTGGCTCCTCGCCCGTAACAAAACGGCGTATATCCCCACCGTTTCGGCCGTACTACGAGCCATCATGTACAGTGCCCACCCTCCTCACGAGTCTACGTGTAGTCCGGACCAAGAAAACATGCCGCTGGCCGTCGATTCGTTACAGATGGAAAAAGCGCAGTCGTCATCGCCACCGTCCATTTCTCTGGTTCCGATTATACCCCTATTGGTTATTGGTTATTGGCTTGCTTGGTAGTGGGCGTAGGGGTAATTATGGGTTGGGATCCGTTTGTTTTGTAATTTTTGGAGCCTGTCGGGTTCACGTGAAGCTAGTGGACAAAAATAAGGGATGATGGAGGGGCTTTTTTGTCTTTTCGCTACACTGTAAATGTCAAATCTGGGTGCTTTTTTTTACTTTTTTTACTCTCTTCCCATTCGCTTTCTTGTGACAAATTTTTCCTTGGGGCTTTCAATTAAACCAGAAAGTAAAAAACTTGCGGTTCTTAAATCA

Coding sequence (CDS)

ATGCCCATTTCTTCATTTCCCCCAAAAATGCTTCATTTCGCCCAAAAATGTCTAATTTGCTTCGTTTTCTTAAGCATTGCTACAAATTTTCATTCAACTTCAGCTGGTTTTCTCATCGAACCGGTTTCCGGCCAAAGCCAGCCACTAAATCCCGGCGAATACTCATCCTCCAACACGGTTCCGGCCTTTCCGGTCCAAACCCAGATGCAAATTTGCCATCTTGATCTCTCCGACGAGCTTTTCGGCGGCGTTAGGGAAGCTTGCGGTCGAGACTTGGACCGGAGTCGGTGCTGTCCTGTTCTAGCCGCGTGGCTTTTTGCCGCTCATGCACGCGCCGCCCTGAAGATTACCACTCCGGCGCCGTCGGACCTTCCGATGATGCCGGATGACTCACAGAAATGCGTGAACTCGCTGCAGGCGTCGCTGTTGAGTAGAAATATAAAGATTCCACAGCCGAATGCGAGCTGCGACGCCGTTTTGTGCTTTTGTGGGATTCGGCTTCATCAAATTAGCTCGTTGTCTTGCCCCGCTGCCTTTAATGTCTCCGGCGACGGCGTTCGCGACGGCTACCGGAACGCCACCCCGACGGCCGCCGTCCGTAATCTTGAAAAGAATTGCCGGAACTCATCTTATTCCGGTTGCACGAAATGCCTTGGCGCCCTTCAAAAGGTTACGGCAACGAAGAAGAACGCCACCAGCGACAGAGTGAGTAAAATGTTCAACAGAGACTGCCAGCTCATGGGGCTGACGTGGCTCCTCGCCCGTAACAAAACGGCGTATATCCCCACCGTTTCGGCCGTACTACGAGCCATCATGTACAGTGCCCACCCTCCTCACGAGTCTACGTGTAGTCCGGACCAAGAAAACATGCCGCTGGCCGTCGATTCGTTACAGATGGAAAAAGCGCAGTCGTCATCGCCACCGTCCATTTCTCTGGTTCCGATTATACCCCTATTGGTTATTGGTTATTGGCTTGCTTGGTAG

Protein sequence

MPISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPAFPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPSDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSSPPSISLVPIIPLLVIGYWLAW

Homology

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BLAST of CmoCh11G019260 vs. ExPASy Swiss-Prot
Match: Q9SUC9 (Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 GN=At4g28100 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 7.7e-111
Identity = 207/314 (65.92%), Postives = 244/314 (77.71%), Query Frame = 0


Query: 15  QKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPAFPVQTQMQICHL 74
           +K L   + L  +  F +  +  L+EPV    QP         NTVPAFPV+TQ Q C L
Sbjct: 2   KKSLTLLILLLCSLLFSTVLSNLLVEPV----QP---------NTVPAFPVETQAQSCRL 61

Query: 75  DLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPS------DLPMMP 134
           DLS+ELFGGV EACGR+LDRSRCCPVLAAWLFAAHAR+AL++  PAP+      D PM P
Sbjct: 62  DLSNELFGGVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKP 121

Query: 135 DDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRD 194
           DDSQKCVN+LQ++LL++ IKIPQPN+SCDA+LCFCGIRLHQISSLSCPAAFNVS      
Sbjct: 122 DDSQKCVNTLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVS-----S 181

Query: 195 GYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQ--KVTATKKNATSDRVSKMFNRDCQL 254
           G++NATPTAAV+NLEK CRNSSYSGCT+CLGALQ  KV    K  T++R +KM ++DCQL
Sbjct: 182 GFKNATPTAAVKNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQL 241

Query: 255 MGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSS 314
           MGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH + CSPDQENMPLAVDSLQ +K+ SSS
Sbjct: 242 MGLTWLLARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSSS 297

Query: 315 PPSISLVPIIPLLV 321
                ++P +PL++
Sbjct: 302 SHLFGVLPFLPLVL 297

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BLAST of CmoCh11G019260 vs. ExPASy TrEMBL
Match: A0A6J1EY13 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=3662 GN=LOC111437232 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.3e-187
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0


Query: 1   MPISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTV 60
           MPISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTV
Sbjct: 1   MPISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTV 60

Query: 61  PAFPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPA 120
           PAFPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPA
Sbjct: 61  PAFPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPA 120

Query: 121 PSDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFN 180
           PSDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFN
Sbjct: 121 PSDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFN 180

Query: 181 VSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMF 240
           VSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMF
Sbjct: 181 VSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMF 240

Query: 241 NRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQME 300
           NRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQME
Sbjct: 241 NRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQME 300

Query: 301 KAQSSSPPSISLVPIIPLLVIGYWLAW 328
           KAQSSSPPSISLVPIIPLLVIGYWLAW
Sbjct: 301 KAQSSSPPSISLVPIIPLLVIGYWLAW 327

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BLAST of CmoCh11G019260 vs. ExPASy TrEMBL
Match: A0A6J1JNJ0 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 GN=LOC111486078 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.8e-179
Identity = 314/318 (98.74%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 10  MLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPAFPVQTQM 69
           ML+FAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQP NPGEYSSSNTVPAFPVQTQM
Sbjct: 1   MLNFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPQNPGEYSSSNTVPAFPVQTQM 60

Query: 70  QICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPSDLPMMPD 129
           QICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPSDLPMMPD
Sbjct: 61  QICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPSDLPMMPD 120

Query: 130 DSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRDG 189
           DSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRDG
Sbjct: 121 DSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRDG 180

Query: 190 YRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMFNRDCQLMGL 249
           YRNATPTA VRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMFNRDCQLMGL
Sbjct: 181 YRNATPTAVVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKMFNRDCQLMGL 240

Query: 250 TWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSSPPS 309
           TWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSSPPS
Sbjct: 241 TWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSSPPS 300

Query: 310 ISLVPIIPLLVIGYWLAW 328
           ISL+PIIPLLVIGYWLAW
Sbjct: 301 ISLIPIIPLLVIGYWLAW 318

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BLAST of CmoCh11G019260 vs. ExPASy TrEMBL
Match: A0A5A7SLX1 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003780 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 291/329 (88.45%), Postives = 301/329 (91.49%), Query Frame = 0


Query: 3   ISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPA 62
           +SSFP K+       LICF+FL IATNFHS+SAGFL+EPVSGQSQPLNPG+YSS NTVPA
Sbjct: 1   MSSFPLKV------PLICFIFLCIATNFHSSSAGFLVEPVSGQSQPLNPGQYSSPNTVPA 60

Query: 63  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAP- 122
           FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKI  PAP 
Sbjct: 61  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKIAAPAPA 120

Query: 123 --SDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 182
             +DLPMMPDDSQKCVNSLQ SLLSRNI+IPQPNASCDAVLCFCGIRLHQISSLSCPAAF
Sbjct: 121 AAADLPMMPDDSQKCVNSLQTSLLSRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 180

Query: 183 NVSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATS-DRVSK 242
           NVSG G   GYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVT TKKN TS DR SK
Sbjct: 181 NVSGGG-NGGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTGTKKNGTSNDRASK 240

Query: 243 MFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQ 302
           MFNRDCQLMGLTWLLARNKT YIPTVSAVLRAIMY+AHPPHESTCSPDQENMPLAVDSLQ
Sbjct: 241 MFNRDCQLMGLTWLLARNKTTYIPTVSAVLRAIMYTAHPPHESTCSPDQENMPLAVDSLQ 300

Query: 303 MEKAQSSSPPSISLVPIIPLLVIGYWLAW 328
           MEKAQSSSPPSISL PIIPLL+  YWLAW
Sbjct: 301 MEKAQSSSPPSISLFPIIPLLISVYWLAW 322

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BLAST of CmoCh11G019260 vs. ExPASy TrEMBL
Match: A0A1S3C1G3 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=LOC103495549 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 291/329 (88.45%), Postives = 301/329 (91.49%), Query Frame = 0


Query: 3   ISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPA 62
           +SSFP K+       LICF+FL IATNFHS+SAGFL+EPVSGQSQPLNPG+YSS NTVPA
Sbjct: 1   MSSFPLKV------PLICFIFLCIATNFHSSSAGFLVEPVSGQSQPLNPGQYSSPNTVPA 60

Query: 63  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAP- 122
           FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKI  PAP 
Sbjct: 61  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKIAAPAPA 120

Query: 123 --SDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 182
             +DLPMMPDDSQKCVNSLQ SLLSRNI+IPQPNASCDAVLCFCGIRLHQISSLSCPAAF
Sbjct: 121 AAADLPMMPDDSQKCVNSLQTSLLSRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 180

Query: 183 NVSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATS-DRVSK 242
           NVSG G   GYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVT TKKN TS DR SK
Sbjct: 181 NVSGGG-NGGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTGTKKNGTSNDRASK 240

Query: 243 MFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQ 302
           MFNRDCQLMGLTWLLARNKT YIPTVSAVLRAIMY+AHPPHESTCSPDQENMPLAVDSLQ
Sbjct: 241 MFNRDCQLMGLTWLLARNKTTYIPTVSAVLRAIMYTAHPPHESTCSPDQENMPLAVDSLQ 300

Query: 303 MEKAQSSSPPSISLVPIIPLLVIGYWLAW 328
           MEKAQSSSPPSISL PIIPLL+  YWLAW
Sbjct: 301 MEKAQSSSPPSISLFPIIPLLISVYWLAW 322

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BLAST of CmoCh11G019260 vs. ExPASy TrEMBL
Match: A0A0A0K5M9 (SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046110 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 287/328 (87.50%), Postives = 300/328 (91.46%), Query Frame = 0


Query: 3   ISSFPPKMLHFAQKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPA 62
           +SSFP K+       LICF+FL IATN HS+SAGFLIEPVSGQSQPLNPG+YSS NTVPA
Sbjct: 1   MSSFPLKV------PLICFIFLCIATNLHSSSAGFLIEPVSGQSQPLNPGQYSSPNTVPA 60

Query: 63  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAP- 122
           FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKI  PAP 
Sbjct: 61  FPVQTQMQICHLDLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKIAAPAPA 120

Query: 123 --SDLPMMPDDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 182
             +DLPMMPDDSQKCVNSLQ SLLSRNI+IPQPNASCDAVLCFCGIRLHQISSLSCPAAF
Sbjct: 121 SAADLPMMPDDSQKCVNSLQTSLLSRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAF 180

Query: 183 NVSGDGVRDGYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTATKKNATSDRVSKM 242
           NVSG G    YRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVT TKKN+++DR SKM
Sbjct: 181 NVSGGG-NGAYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQKVTGTKKNSSNDRASKM 240

Query: 243 FNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQM 302
           FNRDCQLMGLTWLLARNKT YIPTVSAVLRAIMY+AHPPH+STCSPDQENMPLAVDSLQM
Sbjct: 241 FNRDCQLMGLTWLLARNKTTYIPTVSAVLRAIMYTAHPPHQSTCSPDQENMPLAVDSLQM 300

Query: 303 EKAQSSSPPSISLVPIIPLLVIGYWLAW 328
           EKAQSSSPPSISL PIIPLL+  YWLAW
Sbjct: 301 EKAQSSSPPSISLFPIIPLLISVYWLAW 321

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BLAST of CmoCh11G019260 vs. TAIR 10
Match: AT4G28100.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G18050.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 401.7 bits (1031), Expect = 5.5e-112
Identity = 207/314 (65.92%), Postives = 244/314 (77.71%), Query Frame = 0


Query: 15  QKCLICFVFLSIATNFHSTSAGFLIEPVSGQSQPLNPGEYSSSNTVPAFPVQTQMQICHL 74
           +K L   + L  +  F +  +  L+EPV    QP         NTVPAFPV+TQ Q C L
Sbjct: 2   KKSLTLLILLLCSLLFSTVLSNLLVEPV----QP---------NTVPAFPVETQAQSCRL 61

Query: 75  DLSDELFGGVREACGRDLDRSRCCPVLAAWLFAAHARAALKITTPAPS------DLPMMP 134
           DLS+ELFGGV EACGR+LDRSRCCPVLAAWLFAAHAR+AL++  PAP+      D PM P
Sbjct: 62  DLSNELFGGVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKP 121

Query: 135 DDSQKCVNSLQASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRD 194
           DDSQKCVN+LQ++LL++ IKIPQPN+SCDA+LCFCGIRLHQISSLSCPAAFNVS      
Sbjct: 122 DDSQKCVNTLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVS-----S 181

Query: 195 GYRNATPTAAVRNLEKNCRNSSYSGCTKCLGALQ--KVTATKKNATSDRVSKMFNRDCQL 254
           G++NATPTAAV+NLEK CRNSSYSGCT+CLGALQ  KV    K  T++R +KM ++DCQL
Sbjct: 182 GFKNATPTAAVKNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQL 241

Query: 255 MGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKAQSSS 314
           MGLTWLLARNKTAYIPTVSAVLRAIMYS HPPH + CSPDQENMPLAVDSLQ +K+ SSS
Sbjct: 242 MGLTWLLARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSSS 297

Query: 315 PPSISLVPIIPLLV 321
                ++P +PL++
Sbjct: 302 SHLFGVLPFLPLVL 297

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BLAST of CmoCh11G019260 vs. TAIR 10
Match: AT3G18050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G28100.1); Has 67 Blast hits to 66 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 128/331 (38.67%), Postives = 183/331 (55.29%), Query Frame = 0


Query: 38  LIEPVSGQSQPLNPGEYSSS--NTVPAFPVQTQMQICHLDLSDELFGGVREAC-GRDLDR 97
           ++ PVS +   + P    SS   T+PAFP Q+    C LDL ++LF G++ AC G+ L +
Sbjct: 19  ILSPVSPEPVTVQPFRVKSSPPATIPAFPEQSDFSGCPLDLPEDLFHGIKSACSGKKLHK 78

Query: 98  SRCCPVLAAWLFAAHARAALK----------------ITTPAPSDLPMMPDDSQKCVNSL 157
            +CCPVL AWL++A++  AL                 +TTP   D+P++PDDS+ CV+ L
Sbjct: 79  GKCCPVLGAWLYSAYSTTALSRSISAAARNSSSSSAAVTTP-EEDMPLLPDDSETCVDGL 138

Query: 158 QASLLSRNIKIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNVSGDGVRDGYRNATPTAA 217
             SL  R I++ +PN +CD V C+CGIRLH    LSC  AF V+ +G   G         
Sbjct: 139 GKSLRQRGIELTRPNETCDVVYCYCGIRLH---PLSCSEAFRVNDEGRLVG------DER 198

Query: 218 VRNLEKNCRNSSYS---------GCTKCLGALQKVTATKKNAT-------SDRVSKMFNR 277
           V  LE +C + S++         GC KCL +L K+   K + T        +R +KM N+
Sbjct: 199 VDRLETDCLSGSHNNADGFSPLLGCNKCLNSLYKLNPKKTSGTRNPSKEDRNRTAKMHNK 258

Query: 278 DCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHESTCSPDQENMPLAVDSLQMEKA 325
           DC LMGLTWLLA+N+TAY PTV++VLRA+M + H     +C+   + MPLAVDS +    
Sbjct: 259 DCVLMGLTWLLAKNRTAYFPTVTSVLRAVMLN-HDGVPRSCALGSDGMPLAVDSSEF--- 318

The following BLAST results are available for this feature:

BLAST of CmoCh11G019260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUC9	7.7e-111	65.92	Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of CmoCh11G019260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EY13	2.3e-187	100.00	uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=366...	[more]
A0A6J1JNJ0	1.8e-179	98.74	uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A5A7SLX1	1.4e-160	88.45	Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3C1G3	1.4e-160	88.45	uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=L...	[more]
A0A0A0K5M9	1.2e-159	87.50	SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046110 PE=4 ...	[more]

BLAST of CmoCh11G019260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28100.1	5.5e-112	65.92	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G18050.1	2.0e-53	38.67	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043891	SPARK domain	PFAM	PF19160	SPARK	coord: 70..233 e-value: 4.9E-17 score: 62.8
None	No IPR available	PANTHER	PTHR34056:SF3	OS07G0557700 PROTEIN	coord: 16..312
IPR040376	At4g28100-like	PANTHER	PTHR34056	GPI-ANCHORED PROTEIN	coord: 16..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G019260.1	CmoCh11G019260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane