CmoCh11G017320 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G017320
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMajor facilitator superfamily protein
LocationCmo_Chr11: 12254138 .. 12256539 (+)
RNA-Seq ExpressionCmoCh11G017320
SyntenyCmoCh11G017320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTCCTTCTCTCTGCAACTTCAATGAGCATTTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAATTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAGCTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTCATTGGCTACGGCGGTCAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGGTATTATGGTTTCAATCTCTGTTTCAATTTCTGTAATGAAATGAAAATTTTCGCCTATTTCTTGATTTGGGTTGGTTTTAAATTTATAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGTTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAACGAAACAGAGGATTCCCAGTACTTCTGGGTCCTCAACGCCATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCGTATCGATAATTTTCTCCATTGTGTTATTGATTCTCCTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGCAGAGGCGTTGTTGGCGGCTGAGGAAGTGGCGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACAGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGATTTTTTGGTCGGATTTTATCGGGATCGGCATCGGAGTATTTCATCAAGTAAGTAGAATATAATATATCACGACCCATTTTCAAAAAAATAGCTACCATTGATGTGAGATCCCACACATCGGTTGGGGAGGAGAACGAAACACTATTTATAAGAGTGTAGAAACCTCCCGACACGTTTTAAAAACCCTAAGGGCAAGTCCGAAAGTGAAAGCCCAAAGATGACATTATCTACTAGTCGTAGGCTTAGGCCATTACAAATGGTATTAGAGCCTGGGCAATGCAGGAGGCTGAATTCCGAAGGGCTGGACATGAGGTGATATGCCAGGAAAGACGCTGGGCTACAAAGGGGTGGATTTGGTGGGGCCCACATCGATTGGAGAGAGAAACGAGTGCCAGCGAGGACGTTGGGCCCCGAAGGGGGGTGGATTGTGAAATCCCACCTCGGTTGCGGAGGAGAACGAAACACCCTTTATAAGGGCAAAAAATTTCTCCCTAGCATACGCGTTTTAAAAACCTTGAGCACAAGTCCGAAAGTGAAATTCTAAAGAGGACAATATCTGCTAGCGGTGAGCTTGGACAGTTGAATTGTTTATTCATGAACAGGAAAGCAGGGATGGCAAGGCCACTATGGAACGCAGCTTCTCAAATTCTAATGGCAATTGGCTGCATTCTCTTAGCCATTGGCATGCCTAGTTCCTTATACATCGGCTCGATCATTGTCGGTATCTGTTACGGTGTTCGTCTCGCCATCAGTGTCCCGATCGCCTCTGAAATTTTCGGGCTCAAAGACTATGGCATGATCTACAACGTTCTGATCTTCAACCTCCCACTCGGTTCGTTCCTCTTCTCGGGGCTGCTGGCCGGGATTCTCTACGACATGGAGGCGACGAAGACAGAGGAGGGTGGGAATACGTGCATGGGAGAGCATTGTTACAGGCTTGTGTTTGTGGTAATGGCTTGTTCATGTGTGGTTGGGCTTGGCGTGGACTTGCTTTTGTTCATTAGAACTAAGAGATTGTACTCCAAAATAAGAGATGGAAGGTGATAGGTGACAAAGGCCACTTTAAATGCCCTATTTAAATAAATAATACCGCTATTGGATGCGTGTCATGATGGATCCAATATGTTATTAACGTACTAAAATGTGAAAAATTGTCTATCAAAAAGTTCATTTCTATGGTATCTTATTGTCAAGTACTTATGTAAGTATCAGTATCATATGACAAGTG

mRNA sequence

CCGTCCTTCTCTCTGCAACTTCAATGAGCATTTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAATTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAGCTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTCATTGGCTACGGCGGTCAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGTTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAACGAAACAGAGGATTCCCAGTACTTCTGGGTCCTCAACGCCATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCGTATCGATAATTTTCTCCATTGTGTTATTGATTCTCCTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGCAGAGGCGTTGTTGGCGGCTGAGGAAGTGGCGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACAGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGATTTTTTGGTCGGATTTTATCGGGATCGGCATCGGAGTATTTCATCAAGAAAGCAGGGATGGCAAGGCCACTATGGAACGCAGCTTCTCAAATTCTAATGGCAATTGGCTGCATTCTCTTAGCCATTGGCATGCCTAGTTCCTTATACATCGGCTCGATCATTGTCGGTATCTGTTACGGTGTTCGTCTCGCCATCAGTGTCCCGATCGCCTCTGAAATTTTCGGGCTCAAAGACTATGGCATGATCTACAACGTTCTGATCTTCAACCTCCCACTCGGTTCGTTCCTCTTCTCGGGGCTGCTGGCCGGGATTCTCTACGACATGGAGGCGACGAAGACAGAGGAGGGTGGGAATACGTGCATGGGAGAGCATTGTTACAGGCTTGTGTTTGTGGTAATGGCTTGTTCATGTGTGGTTGGGCTTGGCGTGGACTTGCTTTTGTTCATTAGAACTAAGAGATTGTACTCCAAAATAAGAGATGGAAGGTGATAGGTGACAAAGGCCACTTTAAATGCCCTATTTAAATAAATAATACCGCTATTGGATGCGTGTCATGATGGATCCAATATGTTATTAACGTACTAAAATGTGAAAAATTGTCTATCAAAAAGTTCATTTCTATGGTATCTTATTGTCAAGTACTTATGTAAGTATCAGTATCATATGACAAGTG

Coding sequence (CDS)

ATGAGCATTTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAATTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAGCTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTCATTGGCTACGGCGGTCAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGTTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAACGAAACAGAGGATTCCCAGTACTTCTGGGTCCTCAACGCCATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCGTATCGATAATTTTCTCCATTGTGTTATTGATTCTCCTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGCAGAGGCGTTGTTGGCGGCTGAGGAAGTGGCGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACAGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGATTTTTTGGTCGGATTTTATCGGGATCGGCATCGGAGTATTTCATCAAGAAAGCAGGGATGGCAAGGCCACTATGGAACGCAGCTTCTCAAATTCTAATGGCAATTGGCTGCATTCTCTTAGCCATTGGCATGCCTAGTTCCTTATACATCGGCTCGATCATTGTCGGTATCTGTTACGGTGTTCGTCTCGCCATCAGTGTCCCGATCGCCTCTGAAATTTTCGGGCTCAAAGACTATGGCATGATCTACAACGTTCTGATCTTCAACCTCCCACTCGGTTCGTTCCTCTTCTCGGGGCTGCTGGCCGGGATTCTCTACGACATGGAGGCGACGAAGACAGAGGAGGGTGGGAATACGTGCATGGGAGAGCATTGTTACAGGCTTGTGTTTGTGGTAATGGCTTGTTCATGTGTGGTTGGGCTTGGCGTGGACTTGCTTTTGTTCATTAGAACTAAGAGATTGTACTCCAAAATAAGAGATGGAAGGTGA

Protein sequence

MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR
Homology
BLAST of CmoCh11G017320 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.4e-60
Identity = 161/532 (30.26%), Postives = 275/532 (51.69%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           KW  LV+A+W+Q+  G N+ FS YS+ LKS++ +SQ++LN L+VA DLGKAFG  SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
              P S++L   +  G +GYG QWLV++  I  LPY  + +   + G S  W NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIIT-LPYSLVFLCCLLAGLSICWFNTACFIL 162

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
           C+R+F  N      +   +  +S A+++    A+  +  + +L L ++VP  V   A+  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYP 222

Query: 197 LRETPFA-----VDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSIVLLI 256
           +   P        D+   DS  F +LN +AV  +  LL   S    ++ ++ I ++VLL+
Sbjct: 223 VLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL-LSSSSTSSARLNFIGAVVLLV 282

Query: 257 L-LVSPLVI-------PIHSFLKNRGRSG-------------AAAEALLAAEEVAEVEGN 316
             L +PL++       P+ +   N   SG              +  +    E +   +  
Sbjct: 283 FPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEG 342

Query: 317 GNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLM 376
               +G++H+    +  +EFWL ++++  G   GL   NN+GQI  +LG +  ++ +++ 
Sbjct: 343 NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS-TTLVTIY 402

Query: 377 SIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILLAI--GMPSSLYIGSII 436
           S + FFGR+LS +A ++  K+  + R  W A + +   I   LLA+     ++L   + +
Sbjct: 403 SSFSFFGRLLS-AAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATAL 462

Query: 437 VGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEA---- 496
           +G+  G   A +V I S++FG    G+ +N+LI N+P+GS L+ G +A  +Y+  A    
Sbjct: 463 IGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASPDI 522

Query: 497 TKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           T        C+G  CY   FV   C  ++G+   L L+IRTK +Y ++   +
Sbjct: 523 TPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRLEQDK 569

BLAST of CmoCh11G017320 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-06
Identity = 111/510 (21.76%), Postives = 197/510 (38.63%), Query Frame = 0

Query: 31  AGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL-LSGLASDRLPPSLLLLIGS 90
           AG+   FS Y   L + +N +QL++N +S+A  +     + L+G   DR  PS L L   
Sbjct: 94  AGSITAFSLYGPLLLTRLNYTQLRVNEVSIAAGISMYLPVSLAGYLCDRYSPSPLTLFAG 153

Query: 91  LEGLIGYGGQWLVVSKTINP------LPYWQISIFMCMGGNSSTWMNTAVLVTCLRNFRR 150
           +   +GY     V      P       P+W + +     G ++  M  A + TC +NF R
Sbjct: 154 IAFGLGYSLAAFVYKSGPPPDAGGNGWPFWVMVVAFIAIGVATCSMYLAAVTTCAKNFGR 213

Query: 151 NIGSVSGVLKGYVALSTAIF------------TDLCFALFSN-----DPSSFLALLAVVP 210
             G   G++   +A+  A F              LC  L  +     D   +   LA++ 
Sbjct: 214 --GKHKGII---LAVPIAAFGLSGMWQSQVGTYFLCERLKDSNCGDVDVYKYFLFLAILL 273

Query: 211 FAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFS 270
             + +I    LR     VD+E E     ++  A+       LLA      P S V   + 
Sbjct: 274 LTIGVIGTFALR----IVDDEEEK----YIDEAVEELERSGLLAESEFFRPRSEVQAAYG 333

Query: 271 IVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTIVEAMK 330
                   +  V      +    R  A  E     E   E     N ++  +  I  A  
Sbjct: 334 TFSDDHEDNGSVDDQSVTISEELREAARRE----KEREEEERRKKNWLLNYETRIFLADH 393

Query: 331 TVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDV-----------SSFISLMSIWGF 390
           T+  W L + F    G G A +NN+G I   L  D             S+ ++++++   
Sbjct: 394 TM--WWLALGFFLVTGPGEAYLNNLGTIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTST 453

Query: 391 FGRILSGSASEYFI---------------------KKAGMARPLWNAASQILMAIGCILL 450
             R+L+GS S+ F                      ++  ++R  +   S +L+++G +LL
Sbjct: 454 IARLLTGSLSDLFAPTARRHFTVDQETAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLL 513

Query: 451 AIGMPSS----LYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFL 481
           A  +P+      ++ + +VG+ YG   ++   I S ++G++++G  + ++    P     
Sbjct: 514 ASPLPTHHPELSHLTTALVGLGYGSIFSLVPIIISVVWGVENFGTNWGIVAM-FPAAGAA 573

BLAST of CmoCh11G017320 vs. ExPASy Swiss-Prot
Match: P37662 (Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83333 GN=yhjX PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 40/160 (25.00%), Postives = 75/160 (46.88%), Query Frame = 0

Query: 292 NPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDV---SSFIS 351
           N V+ +D+T+ E+M+  ++W+L V FL    +GL V+     I  +L + DV   ++ ++
Sbjct: 198 NGVVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVT 257

Query: 352 LMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSII 411
           ++SI    GR++ G  S+    K    R +       L+ +  +L A     + +     
Sbjct: 258 VISIANLSGRLVLGILSD----KIARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIAC 317

Query: 412 VGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGS 449
           V   +G  + +   + SE FGL +    Y V+     +GS
Sbjct: 318 VAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGS 353

BLAST of CmoCh11G017320 vs. ExPASy TrEMBL
Match: A0A6J1ENR3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435199 PE=4 SV=1)

HSP 1 Score: 989.6 bits (2557), Expect = 5.0e-285
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 516

BLAST of CmoCh11G017320 vs. ExPASy TrEMBL
Match: A0A6J1JP36 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486233 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 507/516 (98.26%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPP SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPP-SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLP SLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFD IPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASEYFIKKAGMARPLWNAASQILMAIG ILLAIGMPSSLYIGSIIVGICYGVRLAIS
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VPIASEIFGLKDYGMIYNVLI NLPLGSFLFSGLLAGILYDMEATK EEGGNTCMG HCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFI+TKRLYSK RDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIKTKRLYSKTRDGR 515

BLAST of CmoCh11G017320 vs. ExPASy TrEMBL
Match: A0A1S3BI29 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 4.0e-226
Identity = 409/513 (79.73%), Postives = 455/513 (88.69%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           M+IF   PP  SSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSV
Sbjct: 25  MNIFRRSPP--SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSV 84

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLL+GL+SDRL  SLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 85  AKDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLC 144

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLA
Sbjct: 145 MGGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLA 204

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LL++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FDSIPNP S 
Sbjct: 205 LLSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 264

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           +S IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E  A+    G P IGEDHT
Sbjct: 265 LSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHT 324

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGY+DVS FISLMSIWGFFGRIL
Sbjct: 325 IVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRIL 384

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASE+FIKKAGM RPLWNAASQILMA+G I+LAI +P SLYIGSI+VGICYGVRLAI+
Sbjct: 385 SGSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAIT 444

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VP+ASE+FGLK+YGMIYNVLI NLPLGSFLFSGLLAGILYDMEAT T+ GGNTC G HCY
Sbjct: 445 VPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCY 504

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIR 514
           RLVFVVMACSC +G  +DL L  RTKRLYSK+R
Sbjct: 505 RLVFVVMACSCGIGFAMDLFLAFRTKRLYSKMR 535

BLAST of CmoCh11G017320 vs. ExPASy TrEMBL
Match: A0A6N2LMK6 (Nodulin-like domain-containing protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS242394 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 2.3e-197
Identity = 361/538 (67.10%), Postives = 432/538 (80.30%), Query Frame = 0

Query: 12  SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLL 71
           S SA KWL  V+AVWVQ+I+GNNYTFSNYS ALKSLMNL+QL+LNNLSVAKD+GKAFGLL
Sbjct: 10  SFSATKWLGFVTAVWVQAISGNNYTFSNYSDALKSLMNLTQLELNNLSVAKDVGKAFGLL 69

Query: 72  SGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNT 131
           +GLASDRLP  ++LLIGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C+GGNS+TWMNT
Sbjct: 70  AGLASDRLPTPVILLIGSIEGLIGYGTQWLVVSRRIQPLPYWQMCIFLCLGGNSTTWMNT 129

Query: 132 AVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCL 191
           AVLVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALFS++P+ FL +LAV+PFAVCL
Sbjct: 130 AVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCAALFSDEPAKFLIMLAVIPFAVCL 189

Query: 192 IAILFLRETPFA--VDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSIVL 251
            AI+FLRETP A  V+ E E+S+YF + N +AV VAV L+A+  IPNP+   S+ F+++L
Sbjct: 190 AAIVFLRETPPAATVEEEKEESKYFNIFNTVAVIVAVYLMAYGFIPNPSHVFSLAFAVIL 249

Query: 252 LILLVSPLVIPIHSFLK----NRGRSGAAAEALL-------------------------- 311
           L+LL SPL +P+H+F+K    NR ++ A  E  +                          
Sbjct: 250 LVLLASPLAVPVHAFIKSWTLNRFKNQADVERQIQEPLLREEKAQEEIQEKPAEESASAV 309

Query: 312 -----AAEEVAEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQI 371
                AAEE A VE    P IGEDHTI EAM T++FW+LFVSFL GVGTGLAVMNNMGQI
Sbjct: 310 VEQPQAAEEKAAVEVKRRPAIGEDHTIFEAMCTLDFWILFVSFLCGVGTGLAVMNNMGQI 369

Query: 372 GLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILL 431
           GLALGY DVS FIS+ SIWGFFGRI+SGS SEY+IKKAG+ RPLWNAASQILMA+G IL+
Sbjct: 370 GLALGYADVSLFISMTSIWGFFGRIVSGSVSEYYIKKAGIPRPLWNAASQILMAVGYILM 429

Query: 432 AIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGL 491
           A+ +P SLYIGS++VGICYGVRLA+SVP ASE+FGLK +G+IYN+LI NLPLGSFLFSGL
Sbjct: 430 AVALPGSLYIGSVVVGICYGVRLAVSVPTASELFGLKYFGLIYNILILNLPLGSFLFSGL 489

Query: 492 LAGILYDMEATKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTKRLYSKI 513
           LAG+LYD EAT T  GGNTC+G HCYRLVF++MA +CVVG G+D+LL IRTK++Y+KI
Sbjct: 490 LAGLLYDAEATPTPGGGNTCVGAHCYRLVFIIMAVACVVGFGLDVLLGIRTKKIYNKI 547

BLAST of CmoCh11G017320 vs. ExPASy TrEMBL
Match: A0A498JRG7 (Nodulin-like domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_009961 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 2.0e-196
Identity = 357/535 (66.73%), Postives = 426/535 (79.63%), Query Frame = 0

Query: 11  SSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL 70
           S SSA KWL  V+AVWVQSI+GNNYTFSNYSAALK+LM L+QL+LNNLSVAKD+GKAFGL
Sbjct: 72  SVSSATKWLGFVTAVWVQSISGNNYTFSNYSAALKTLMGLTQLELNNLSVAKDVGKAFGL 131

Query: 71  LSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMN 130
           L+GLASD+LP  ++LLIGS+EGL+GYG QWLVVS+ I PL YWQ+ IF+CMGGNS+TWMN
Sbjct: 132 LAGLASDKLPTWVILLIGSVEGLVGYGAQWLVVSERIKPLSYWQMCIFLCMGGNSTTWMN 191

Query: 131 TAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVC 190
           TA+LVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALF+NDP+SFL +LAV PFAVC
Sbjct: 192 TAILVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCAALFNNDPASFLLMLAVTPFAVC 251

Query: 191 LIAILFLRETP--FAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSIV 250
           L A+LFL E P   +   +  DS+YFWV+NA+A+ VA+ LLA+D IPNP++ +S+IFS++
Sbjct: 252 LTAMLFLHEVPPSSSSSEDNRDSRYFWVVNAVAICVALYLLAYDFIPNPSNLLSLIFSVI 311

Query: 251 LLILLVSPLVIPIHSF---------------------------LKNRGRSGAAAEALLAA 310
           LLILL SPL IP ++F                           L+         +  +AA
Sbjct: 312 LLILLASPLAIPAYAFFTAWASKPDLVASESGPDPNTRSDEPLLRQTAADDTQHKPEVAA 371

Query: 311 EEVAEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYD 370
                VE    PV+GEDHTI +AM  ++FW+LFVSFL GVGTGLAVMNNMGQIGLALGY 
Sbjct: 372 AAAEVVEAKRRPVLGEDHTIFDAMVKLDFWILFVSFLCGVGTGLAVMNNMGQIGLALGYV 431

Query: 371 DVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSS 430
           DVS F+SL SIWGFFGRI SG  SEYFI+KA + RPLWNAASQILMA+G IL+AI MP S
Sbjct: 432 DVSIFVSLTSIWGFFGRIGSGMISEYFIRKAAIPRPLWNAASQILMAVGYILMAIAMPGS 491

Query: 431 LYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYD 490
           LYIGS IVGICYGVRLA++VP+ASE+FGLK YG+IYN+LI NLPLGSFLFSGLLAG+LYD
Sbjct: 492 LYIGSTIVGICYGVRLAVTVPVASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGLLYD 551

Query: 491 MEATKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           MEAT TE GGNTC+G HCYRLVFV+MA +CV+G G+D+LL IRTK LY++I+ G+
Sbjct: 552 MEATTTEGGGNTCVGAHCYRLVFVIMAIACVIGFGLDILLSIRTKNLYTRIQAGK 606

BLAST of CmoCh11G017320 vs. NCBI nr
Match: XP_022928343.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 989.6 bits (2557), Expect = 1.0e-284
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 516

BLAST of CmoCh11G017320 vs. NCBI nr
Match: XP_023529669.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 507/516 (98.26%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGM SSLYIGSIIVGICYGVRLAIS
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMSSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           V IASE FGLKDYGMIYNVLI NLPLGS LFSGLLAGILYDMEATKTEEGGNTCMG HCY
Sbjct: 421 VSIASETFGLKDYGMIYNVLILNLPLGSCLFSGLLAGILYDMEATKTEEGGNTCMGAHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 516

BLAST of CmoCh11G017320 vs. NCBI nr
Match: XP_022989043.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 964.1 bits (2491), Expect = 4.7e-277
Identity = 507/516 (98.26%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPP SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPP-SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLP SLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFD IPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGSASEYFIKKAGMARPLWNAASQILMAIG ILLAIGMPSSLYIGSIIVGICYGVRLAIS
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VPIASEIFGLKDYGMIYNVLI NLPLGSFLFSGLLAGILYDMEATK EEGGNTCMG HCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFI+TKRLYSK RDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIKTKRLYSKTRDGR 515

BLAST of CmoCh11G017320 vs. NCBI nr
Match: KAG7015269.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 874.8 bits (2259), Expect = 3.7e-250
Identity = 467/516 (90.50%), Postives = 468/516 (90.70%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRR+IGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRSIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWG      
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWG------ 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
                                                   SLYIGSIIVGICYGVRLAIS
Sbjct: 361 ----------------------------------------SLYIGSIIVGICYGVRLAIS 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMG HCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGAHCY 470

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIRDGR 517
           RLVFVVMACSCVVGLGVDLLLFIRTKRLYSK RDGR
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKTRDGR 470

BLAST of CmoCh11G017320 vs. NCBI nr
Match: XP_038887463.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 821.2 bits (2120), Expect = 4.9e-234
Identity = 423/513 (82.46%), Postives = 465/513 (90.64%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFH PPP  SSSA+KWL LV+AVWVQSI+GNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHRPPP--SSSALKWLGLVTAVWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLL+G+ASDRL  SLLLLIGS+EGLIGYG QWLVVS+ INPLPYWQ+ IF+C
Sbjct: 61  AKDLGKAFGLLAGIASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKINPLPYWQMCIFLC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRRN G+VSG+LKGY+ALSTAIFTD+C ALFS++PSSFLA
Sbjct: 121 MGGNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDICSALFSDNPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LL++VPFAVCL AILFLRE P + DNETEDS+YFW+LNA++V VAV+LLAFDSIPNPTSS
Sbjct: 181 LLSLVPFAVCLTAILFLREIPCSADNETEDSRYFWILNAVSVIVAVTLLAFDSIPNPTSS 240

Query: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300
           +S IFSIVLLI L SPL IP+HSFLKNRGRSG  AEALLA E VAE    G PVIGEDHT
Sbjct: 241 ISRIFSIVLLIFLASPLAIPLHSFLKNRGRSGEIAEALLADESVAEGVVEGQPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFW+LF SFL GVGTGLAVMNNMGQIGLALGYDDVS FISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWILFGSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420
           SGS SE+FI+K GM RPLWNAASQ+LMA+G ILLAI MP SLYIGSIIVGICYGVRLAI+
Sbjct: 361 SGSVSEHFIRKDGMPRPLWNAASQVLMALGYILLAIAMPGSLYIGSIIVGICYGVRLAIT 420

Query: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480
           VP+ASE+FGLK+YGMIYNVLI NLPLGSFLFSGLLAGILYDMEAT+ + GGN+C+G HCY
Sbjct: 421 VPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATRRKGGGNSCVGGHCY 480

Query: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIR 514
           RLVFVVMACSCV+G  +DL L  RTKRLYSK+R
Sbjct: 481 RLVFVVMACSCVIGFAMDLFLASRTKRLYSKMR 511

BLAST of CmoCh11G017320 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 681.4 bits (1757), Expect = 5.6e-196
Identity = 357/527 (67.74%), Postives = 424/527 (80.46%), Query Frame = 0

Query: 11  SSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL 70
           SSSSA+KWL  V+AVWVQSI+GNNYTFSNYS ALKSLMNL+QL+LNNLSVAKD+GKAFG+
Sbjct: 8   SSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFGI 67

Query: 71  LSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMN 130
           L+GLASDRLP  ++LLIG  EGL+GYG QWLVVS+TI P+PYWQ+ IF+CMGGNS+TWMN
Sbjct: 68  LAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNSTTWMN 127

Query: 131 TAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVC 190
           TAVLVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALFSNDP+SFL LLAVVPFAVC
Sbjct: 128 TAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVVPFAVC 187

Query: 191 LIAILFLRETPFA--VDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSIV 250
           L A+ FLRE P A     E E+++YF + N +AV VAV L ++D I   T   S+ F+ +
Sbjct: 188 LTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVFSVAFASI 247

Query: 251 LLILLVSPLVIPIHSFLK--NRGRSG---------------AAAE------ALLAAEEVA 310
           LL LL SP+ IP HSF+K  N G                  AAAE      A +AAEE  
Sbjct: 248 LLFLLASPIAIPFHSFIKSLNYGEQDDLEGRIQEPLLRSEIAAAEKEVIVVAAVAAEE-- 307

Query: 311 EVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSS 370
           E +    PV+GEDHTI+EA+ TV+FW+LFVSFL GVGTGLAVMNNMGQIGLALGY +VS 
Sbjct: 308 EEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTNVSI 367

Query: 371 FISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIG 430
           F+S+ SIWGFFGRILSG+ SEYF+KKAG  RPLWNAASQILMA+G IL+A+ +P+SLYIG
Sbjct: 368 FVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVPNSLYIG 427

Query: 431 SIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEAT 490
           S++VG+CYGVRLAI+VP ASE+FGLK YG+IYN+L+ NLPLGSFLFSGLLAG LYD EAT
Sbjct: 428 SMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDAEAT 487

Query: 491 KTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTKRLYSKI 513
            T  GGNTC+G HCYRL+F+VMA + V+G+G+DL+L  RTK +Y+KI
Sbjct: 488 PTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYAKI 532

BLAST of CmoCh11G017320 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 5.5e-191
Identity = 350/546 (64.10%), Postives = 429/546 (78.57%), Query Frame = 0

Query: 11  SSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL 70
           SSSSA+KWL  V+AVWVQSI+GNNYTFSNYS ALKSLMNL+QL+LN+LSVAKD+GKAFG+
Sbjct: 10  SSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAFGI 69

Query: 71  LSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMN 130
           L+GLASDRL   ++LLIGS EGL+GYG QWLVVS+TI P+PYWQ+ +F+CMGGNS+TWMN
Sbjct: 70  LAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTWMN 129

Query: 131 TAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVC 190
           TAVLVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALFS+DP+SFL LL+VVPFAVC
Sbjct: 130 TAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFAVC 189

Query: 191 LIAILFLRETP----FAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFS 250
           L A+ FLRE P    FA DN  E+S+YF V N +AV VAV L ++D I   T + SI F+
Sbjct: 190 LTAVFFLREIPPSTTFAEDN--EESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFSIAFA 249

Query: 251 IVLLILLVSPLVIPIHSFLKNRG---------------RSGA---AAEALLAAEEVA--- 310
            +LLILL SP+ +P H+F++++                RSG+     E ++ A   A   
Sbjct: 250 SILLILLASPVAVPFHAFIRSKVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAAAAADNE 309

Query: 311 -----------EVEGNG--------NPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLA 370
                      E E +G         PV+GE+HTI+EAM TV+FW+LFVSFL GVGTGLA
Sbjct: 310 LPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFLCGVGTGLA 369

Query: 371 VMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQIL 430
           VMNNMGQIGLALGY DVS F+S+ SIWGFFGRILSG+ SE+FIKKAG  RPLWNAA+QI+
Sbjct: 370 VMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLWNAAAQII 429

Query: 431 MAIGCILLAIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPL 490
           MA+G +L+A+ +P SLYIGS++VG+CYGVRLAI+VP ASE+FGLK YG+IYN+LI N+PL
Sbjct: 430 MAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILILNMPL 489

Query: 491 GSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTK 513
           GSFLFSGLLAG+LYD EAT T  GGNTC+G HC+R+VF+VMA + ++G+G+DLLL  RTK
Sbjct: 490 GSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLDLLLAYRTK 549

BLAST of CmoCh11G017320 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 8.4e-83
Identity = 188/563 (33.39%), Postives = 297/563 (52.75%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           +WL  V+A+W+QS AG  Y F + S  +KS +N +Q QL+ L VAKDLG + G L+G  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
           + LP    LL+GS++ L+GYG  WL+V+     LP W + I + +G N  T+ NTA LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
            ++NF ++ G V G+LKG+  L  AI + +   + S+D +S + ++AV P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 197 LRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLA---FDSIPNPTSSVSIIFSIVLLILL 256
           +R         + D+  F V+ A+ + +A  L+A    +   + + S+ I F++VL  +L
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAIL 255

Query: 257 VSPLVIPIHS--FLKNRGRSGAAAEALL-------------------------------- 316
           + P+ IPI +  F  +        E LL                                
Sbjct: 256 LVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPKE 315

Query: 317 -----------------------AAEEVAEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVS 376
                                  AAE    V+    P  GED T+ +A+   +FWL+F S
Sbjct: 316 VDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFFS 375

Query: 377 FLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMAR 436
            L G G+GL V++N+GQ+  +LGYD+   F+S++SIW F GRI  G  SE  ++     R
Sbjct: 376 LLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIVRDYAYPR 435

Query: 437 PLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMI 496
           P+  A +Q++M++G I  A G P +++IG++++G+ YG   AI    ASE+FGLK +G +
Sbjct: 436 PVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKKFGAL 495

Query: 497 YNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGG-------NTCMGEHCYRLVFVVMAC 513
           YN L    P GS +FSGL+A  +YD EA +  +G          C G  CY L  ++M+ 
Sbjct: 496 YNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFLTSLIMSG 555

BLAST of CmoCh11G017320 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 2.3e-80
Identity = 178/558 (31.90%), Postives = 292/558 (52.33%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           +WL  V+A+W+QS AG  Y F + S  +KS +N +Q +L+ L VAKDLG + G ++G  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
           + LP    LL+G+++ LIGYG  WL+V+     LP W + + + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
            ++NF ++ G V G+LKG+  L  AI + +   + S++P+S + ++AV P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 197 LRETPFAVDNETEDSQYFWVLNAIAVAVA---VSLLAFDSIPNPTSSVSIIFSIVLLILL 256
           +R           D   F  +  + + +A   +S++    +   + +V  +F+IVL ++L
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 255

Query: 257 VSPLVIPIHS--FLKNRGRSGAAAEALL-------------------------------- 316
           V P+++PI +  F +         E L+                                
Sbjct: 256 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 315

Query: 317 ------------------AAEEVAEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGV 376
                             AAE    V     P  GED T+ +A+   +FWL+F S L G 
Sbjct: 316 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 375

Query: 377 GTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNA 436
           G+GL V++N+GQ+  +LGYD+    +S++SIW F GRI  G  SE  ++     RP+  A
Sbjct: 376 GSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAMA 435

Query: 437 ASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLI 496
            +Q++M++G I  A G P ++YIG++++G+ YG   AI    ASE+FGLK +G +YN L 
Sbjct: 436 VAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFLT 495

Query: 497 FNLPLGSFLFSGLLAGILYDMEATKTEEG-------GNTCMGEHCYRLVFVVMACSCVVG 513
              P GS +FSG++A  +YD EA +   G          C G  C+ L  ++M+  C++ 
Sbjct: 496 LANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCIIA 555

BLAST of CmoCh11G017320 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 8.7e-80
Identity = 185/546 (33.88%), Postives = 292/546 (53.48%), Query Frame = 0

Query: 12  SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLL 71
           S S   W+ L +A WVQ  AG+  TF  YS+ALKS++  SQ Q+  L VA DLG+  GLL
Sbjct: 8   SGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDLGENMGLL 67

Query: 72  SGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNT 131
            G AS++LPP  +LLIG+    +G+G  WL VS+ +  LP+W + + + +  NS++W  T
Sbjct: 68  PGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATNSNSWFGT 127

Query: 132 AVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCL 191
           A LVT +RNF  + G V+G+LKGY+ +S A FT L   +  +     L  L V    +CL
Sbjct: 128 ASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTVGIPVICL 187

Query: 192 IAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSVSIIFSI---- 251
             + F+R    A   +  +  YF    A  +  ++   A+  +    S V I+ SI    
Sbjct: 188 TVMYFIRPCIPATGEDPSEPMYF----AFLLVTSILFAAYLVVTTVLSEVFILPSILKYV 247

Query: 252 ---VLLILLVSPLVIPIHS--FLKNRGRSGAAAEALLAAEE------------------- 311
              ++++LL+SPL +PI    F  N   S   +   LA EE                   
Sbjct: 248 LVAIMVLLLLSPLAVPIKMTLFRSNAKSSPLGSSDNLAKEEGTHEEPLLTPSTSASNLGP 307

Query: 312 -------------VAEVEG----NGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLA 371
                        +AE EG       P  GED    +     +FWLL+  +  G+G+G+ 
Sbjct: 308 IFEGDDESDMEILLAEAEGAVKKKRKPRRGEDFKFGQVFVKADFWLLWFVYFLGMGSGVT 367

Query: 372 VMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIKKAGMARPLWNAASQIL 431
           V NN+ QIG A G  D +  + L S + F GR+ SG+ SE+F++   + R LW  A+Q++
Sbjct: 368 VSNNLAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTLPRTLWMGAAQLV 427

Query: 432 MAIGCILLAIGMPSSLYIGSIIVGICYGVRLAISVPIASEIFGLKDYGMIYNVLIFNLPL 491
           M    +L A+ +  ++Y+ + ++GIC G +  +S+   SE+FGL+ +G+ +N ++   PL
Sbjct: 428 MVFTFLLFAMAIDHTIYVATALIGICMGFQF-LSIATISELFGLRHFGINFNFILLGNPL 487

Query: 492 GSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCYRLVFVVMACSCVVGLGVDLLLFIRTK 513
           G+ +FS +LAG +YD EA K  +G  TC+G  C+R+ F+V+A  C +G  + ++L +R +
Sbjct: 488 GATIFSAILAGYIYDKEADK--QGKMTCIGPDCFRVTFLVLAGVCGLGTLLSIILTVRIR 546

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E14.4e-6030.26Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q5AXV11.2e-0621.76Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / ... [more]
P376621.5e-0425.00Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83... [more]
Match NameE-valueIdentityDescription
A0A6J1ENR35.0e-285100.00protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JP362.3e-27798.26protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486... [more]
A0A1S3BI294.0e-22679.73protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 ... [more]
A0A6N2LMK62.3e-19767.10Nodulin-like domain-containing protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS... [more]
A0A498JRG72.0e-19666.73Nodulin-like domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_00996... [more]
Match NameE-valueIdentityDescription
XP_022928343.11.0e-284100.00protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_023529669.11.1e-27898.26protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
XP_022989043.14.7e-27798.26protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
KAG7015269.13.7e-25090.50Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_038887463.14.9e-23482.46protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G16660.15.6e-19667.74Major facilitator superfamily protein [more]
AT4G34950.15.5e-19164.10Major facilitator superfamily protein [more]
AT3G01930.28.4e-8333.39Major facilitator superfamily protein [more]
AT5G14120.12.3e-8031.90Major facilitator superfamily protein [more]
AT1G80530.18.7e-8033.88Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 303..509
e-value: 8.3E-6
score: 27.3
coord: 15..208
e-value: 5.6E-7
score: 31.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 8..507
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 17..261
e-value: 1.7E-85
score: 286.5
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 10..279
coord: 282..513
NoneNo IPR availablePANTHERPTHR21576:SF106MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 10..279
coord: 282..513
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 17..495
e-value: 7.10164E-116
score: 345.391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G017320.1CmoCh11G017320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane