CmoCh11G015750 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh11G015750 vs. ExPASy Swiss-Prot
Match: A7IC75 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) OX=78245 GN=gpmA PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-07
Identity = 44/148 (29.73%), Postives = 69/148 (46.62%), Query Frame = 0

Query: 105 DRVVMEVFLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFS 164
           DR+++   L+ HG+ E NL++   G R P+  LT +G  +A+     LK++G++F+  F+
Sbjct: 3   DRILV---LVRHGQSEWNLKNLFTGWRDPD--LTEQGVSEAKRAGALLKAEGLKFDVAFT 62

Query: 165 SPLDRARSMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKP--ETLSFIDRI 224
           S L RA+     +  EM        +  AL E  +G      + D      +    I R 
Sbjct: 63  SDLTRAQKTLGLILGEMGQEGVPTTRNVALNERDYGDLAGLNKDDARAKWGDDQVHIWRR 122

Query: 225 QPDFAAPAGESLRQVEFRMVHFLNATIL 251
             D A P GESLR    R + +    IL
Sbjct: 123 SYDIAPPGGESLRDTVARTLPYYVQEIL 145

BLAST of CmoCh11G015750 vs. ExPASy Swiss-Prot
Match: B0UBD4 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Methylobacterium sp. (strain 4-46) OX=426117 GN=gpmA PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-07
Identity = 46/149 (30.87%), Postives = 71/149 (47.65%), Query Frame = 0

Query: 113 LISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARS 172
           L  HG+ E NL++   G R P+  LT  G  +ARA    LK++G+RF+  F+S L RA+ 
Sbjct: 7   LARHGQSEWNLKNLFTGWRDPD--LTEVGIAEARAAGRRLKAKGIRFDICFTSALTRAQR 66

Query: 173 MAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYK--PETLSFIDRIQPDFAAPA 232
            AA + +E+   +      +AL E  +G      + D      +    I R   D   P 
Sbjct: 67  TAALILEELGQPDLPTIADEALNERDYGDLSGLNKDDARARWGKDQVHIWRRSYDVPPPG 126

Query: 233 GESLRQVEFRMVHFLNATILELPEKLRSD 260
           GESL+    R++ +    I  LP  +R +
Sbjct: 127 GESLKDTVARVLPYTMREI--LPRVMRGE 151

BLAST of CmoCh11G015750 vs. ExPASy Swiss-Prot
Match: Q98DM0 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) OX=266835 GN=gpmA PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.5e-07
Identity = 38/131 (29.01%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 113 LISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARS 172
           L+ HG+ E NL++   G R  +  LT +G  +A+A    LK++G++F+  F+S L RA+ 
Sbjct: 7   LVRHGQSEWNLKNLFTGWR--DVDLTEQGHAEAKAAGQKLKARGLKFDIAFTSALSRAQK 66

Query: 173 MAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKP--ETLSFIDRIQPDFAAPA 232
               +   +  S+ +  +  AL E  +G      + D  K   E    + R   D + P 
Sbjct: 67  TCQHILDAVGQSDLKTIRDQALNERDYGDLSGLNKDDARKKWGEEQVHVWRRSYDVSPPG 126

Query: 233 GESLRQVEFRM 242
           GESL+    R+
Sbjct: 127 GESLKDTGARV 135

BLAST of CmoCh11G015750 vs. ExPASy Swiss-Prot
Match: A6WYJ2 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=gpmA PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.3e-06
Identity = 42/152 (27.63%), Postives = 70/152 (46.05%), Query Frame = 0

Query: 113 LISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARS 172
           L+ HG+ E NL++   G R P   LT +G  +A+A    LK+ G++F+  ++S L RA+ 
Sbjct: 7   LVRHGQSEWNLKNLFTGWRDP--GLTEQGHAEAKAAGQRLKAAGLKFDIAYTSALSRAQV 66

Query: 173 MAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKP--ETLSFIDRIQPDFAAPA 232
               +  ++  S+ +  +  AL E  +G      + D      E    I R   D   P 
Sbjct: 67  TCQHILDQLGQSDLKTIRDQALNERDYGDLSGLNKDDARAKWGEEQVHIWRRSYDVPPPG 126

Query: 233 GESLRQVEFRMVHFLNATILELPEKLRSDVAI 263
           GESL+    R+  +   T+   P  LR +  +
Sbjct: 127 GESLKDTGARVWPYYLHTV--QPHVLRGETVL 154

BLAST of CmoCh11G015750 vs. ExPASy Swiss-Prot
Match: P36623 (Phosphoglycerate mutase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpm1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-06
Identity = 40/140 (28.57%), Postives = 67/140 (47.86%), Query Frame = 0

Query: 113 LISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARS 172
           L  HGE E N  +   G + P  AL+  G ++A+     LKS+G +F+  F+S L RA+ 
Sbjct: 12  LTRHGESEWNKLNLFTGWKDP--ALSETGIKEAKLGGERLKSRGYKFDIAFTSALQRAQK 71

Query: 173 MAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFID--RIQPDFAAPA 232
               + +E+     +  K++ L E  +G  +   + D  K      +   R   D A P 
Sbjct: 72  TCQIILEEVGEPNLETIKSEKLNERYYGDLQGLNKDDARKKWGAEQVQIWRRSYDIAPPN 131

Query: 233 GESLRQVEFRMVHFLNATIL 251
           GESL+    R++ +  +TI+
Sbjct: 132 GESLKDTAERVLPYYKSTIV 149

BLAST of CmoCh11G015750 vs. ExPASy TrEMBL
Match: A0A6J1ES06 (uncharacterized protein LOC111435334 OS=Cucurbita moschata OX=3662 GN=LOC111435334 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.1e-229
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 60
           MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL
Sbjct: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 60

Query: 61  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 120
           SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE
Sbjct: 61  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 120

Query: 121 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 180
           LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE
Sbjct: 121 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 180

Query: 181 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 240
           MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR
Sbjct: 181 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 240

Query: 241 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 300
           MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH
Sbjct: 241 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 300

Query: 301 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 360
           GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT
Sbjct: 301 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 360

Query: 361 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 361 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 405

BLAST of CmoCh11G015750 vs. ExPASy TrEMBL
Match: A0A6J1JQY8 (uncharacterized protein LOC111486673 OS=Cucurbita maxima OX=3661 GN=LOC111486673 PE=4 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.2e-225
Identity = 401/407 (98.53%), Postives = 402/407 (98.77%), Query Frame = 0

Query: 1   MGSAQSGQVAEER--EEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP 60
           MGSAQSGQVAEER  EEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP
Sbjct: 1   MGSAQSGQVAEEREEEEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP 60

Query: 61  QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE 120
           QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE
Sbjct: 61  QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE 120

Query: 121 YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC 180
           YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC
Sbjct: 121 YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC 180

Query: 181 QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVE 240
           QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIY PETLSFIDRIQPDFAAPAGESLRQVE
Sbjct: 181 QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYTPETLSFIDRIQPDFAAPAGESLRQVE 240

Query: 241 FRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQ 300
           FRMVHFLNATILELPEKLRSD+AIRPNETLQMSHELTNSI DRDAHSLQSSPHWDLPHRQ
Sbjct: 241 FRMVHFLNATILELPEKLRSDIAIRPNETLQMSHELTNSILDRDAHSLQSSPHWDLPHRQ 300

Query: 301 RHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL 360
           RHGI RKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL
Sbjct: 301 RHGIVRKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL 360

Query: 361 LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 361 LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 407

BLAST of CmoCh11G015750 vs. ExPASy TrEMBL
Match: A0A6J1J699 (uncharacterized protein LOC111482146 OS=Cucurbita maxima OX=3661 GN=LOC111482146 PE=4 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.4e-196
Identity = 361/427 (84.54%), Postives = 379/427 (88.76%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEED--------------DDGDDNVEPASGELENYLVKKVLEQEPEM 60
           MGSA+S QVAEEREE++              DDGDDNVEP   ELENYLVKKVLEQEPEM
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEDDDGDDGDDNVEPVRVELENYLVKKVLEQEPEM 60

Query: 61  LPCHALASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDR 120
           LPCHALASPLSPQLSSLGTPRLGP IKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDR
Sbjct: 61  LPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDR 120

Query: 121 VVMEVFLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP 180
           VVMEVFLISHGE ELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP
Sbjct: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP 180

Query: 181 LDRARSMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDF 240
           LDRARSMA SVCQE+NF+EEQIQ +DALTEMS GHWE CLRS+IY PETLSFIDR+QPDF
Sbjct: 181 LDRARSMAVSVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQPDF 240

Query: 241 AAPAGESLRQVEFRMVHFLNATILELPEKLRSD-VAIRPNETLQMSHELTNSIHDRDAHS 300
           + PAGESLRQVEFRMVHFLN TILELPEKLRSD V+  PNETLQ+SHELTNSIHDRD+ S
Sbjct: 241 SPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHDRDSQS 300

Query: 301 LQSSPHWDLPHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDA-------PLNIRN 360
           L SS  WDLP RQRHGI RKKSGKSRLQTVTTT EHEA+DE+SPRDA        L IRN
Sbjct: 301 LPSS-QWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEADDELSPRDATHQSTLNDLRIRN 360

Query: 361 SSSCTTCIGIFTHSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMND 406
           SSSCTTCI +FTHSIPIKCLLTGLLECSL MLHKFCI+DSSVTVLQHSLKSGWQIKT+ND
Sbjct: 361 SSSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHKFCIEDSSVTVLQHSLKSGWQIKTLND 420

BLAST of CmoCh11G015750 vs. ExPASy TrEMBL
Match: A0A6J1F368 (uncharacterized protein LOC111441774 OS=Cucurbita moschata OX=3662 GN=LOC111441774 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.9e-195
Identity = 359/430 (83.49%), Postives = 378/430 (87.91%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEED-----------------DDGDDNVEPASGELENYLVKKVLEQE 60
           MGSA+S QVAEEREE++                 DDGDDNVEP   ELENYLVKKVLEQE
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEEEEDDDGDDGDDNVEPVRAELENYLVKKVLEQE 60

Query: 61  PEMLPCHALASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSID 120
           PEMLPCHALASPLSPQLSSLGTPRLGP IKVWDPYNVLAPPPQLPPPP+FTRSFSSSSID
Sbjct: 61  PEMLPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSID 120

Query: 121 DDRVVMEVFLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVF 180
           DDRVVMEVFLISHGE ELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVF
Sbjct: 121 DDRVVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVF 180

Query: 181 SSPLDRARSMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQ 240
           SSPLDRARSMA SVCQE+NF+EEQIQ +DALTEMS GHWE CLRS+IY PETLSFIDR+Q
Sbjct: 181 SSPLDRARSMAISVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQ 240

Query: 241 PDFAAPAGESLRQVEFRMVHFLNATILELPEKLRSD-VAIRPNETLQMSHELTNSIHDRD 300
           PDF+ PAGESLRQVEFRMVHFLN TILELPEKLRSD V+  PNETLQ+SHELTNSIH+RD
Sbjct: 241 PDFSPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHNRD 300

Query: 301 AHSLQSSPHWDLPHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDA-------PLN 360
           + SL SS  WDLP RQRHGI RKKSGKSRLQTVTTT EHE +DE+SPRDA        L 
Sbjct: 301 SQSLPSS-QWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEVDDELSPRDATHQSTLNDLR 360

Query: 361 IRNSSSCTTCIGIFTHSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKT 406
           IRNSSSCTTCI +FTHSIPIKCLLTGLLECSL MLHKFCI+DSSVTVLQHSLKSGWQIKT
Sbjct: 361 IRNSSSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHKFCIEDSSVTVLQHSLKSGWQIKT 420

BLAST of CmoCh11G015750 vs. ExPASy TrEMBL
Match: A0A1S3BNZ5 (uncharacterized protein LOC103491727 OS=Cucumis melo OX=3656 GN=LOC103491727 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.2e-194
Identity = 353/422 (83.65%), Postives = 374/422 (88.63%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEE---------DDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHA 60
           MGSA S Q+AEEREEE         +DDGDDNVEPA  EL NYLVKKV+EQEPEMLPCHA
Sbjct: 1   MGSAHSVQLAEEREEEEEEEEEDDDEDDGDDNVEPARAELGNYLVKKVIEQEPEMLPCHA 60

Query: 61  LASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQ SS+GTPRLGP IKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSIGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRAR 180
           FLISHGE ELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+SSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAG 240
           SMA SVCQEMNF EEQIQ +DALTEMS GHWE CLRS+IY PETL+FIDRIQPDF+ PAG
Sbjct: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPAG 240

Query: 241 ESLRQVEFRMVHFLNATILELPEKLRSDVAI-RPNETLQMSHELTNSIHDRDAHSLQSSP 300
           ESLRQVEFRMV FLN TILELPEKLRSDV + RPN+TLQMSHELTNSI DRD HSL  SP
Sbjct: 241 ESLRQVEFRMVQFLNGTILELPEKLRSDVLLHRPNDTLQMSHELTNSIRDRDGHSL-PSP 300

Query: 301 HWDLPHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDA-------PLNIRNSSSCT 360
           HWDLPHRQR GI R+KSGKSRLQTVT+T EH+ E+E+SP DA        L+IRNSSSCT
Sbjct: 301 HWDLPHRQRQGITRRKSGKSRLQTVTSTGEHDVEEEVSPHDATHQSTLNDLSIRNSSSCT 360

Query: 361 TCIGIFTHSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLR 406
           TCIGIFTH+IPIKCLLTGLLECSL MLHKFCI+DSSVTVLQHSLKSGWQIKT+NDTAHLR
Sbjct: 361 TCIGIFTHAIPIKCLLTGLLECSLVMLHKFCIEDSSVTVLQHSLKSGWQIKTLNDTAHLR 420

BLAST of CmoCh11G015750 vs. NCBI nr
Match: KAG7022627.1 (hypothetical protein SDJN02_16361, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805.4 bits (2079), Expect = 2.2e-229
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 60
           MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL
Sbjct: 9   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 68

Query: 61  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 120
           SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE
Sbjct: 69  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 128

Query: 121 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 180
           LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE
Sbjct: 129 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 188

Query: 181 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 240
           MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR
Sbjct: 189 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 248

Query: 241 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 300
           MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH
Sbjct: 249 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 308

Query: 301 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 360
           GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT
Sbjct: 309 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 368

Query: 361 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 369 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 413

BLAST of CmoCh11G015750 vs. NCBI nr
Match: XP_022928565.1 (uncharacterized protein LOC111435334 [Cucurbita moschata])

HSP 1 Score: 805.4 bits (2079), Expect = 2.2e-229
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 60
           MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL
Sbjct: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSPQL 60

Query: 61  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 120
           SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE
Sbjct: 61  SSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGEYE 120

Query: 121 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 180
           LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE
Sbjct: 121 LNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVCQE 180

Query: 181 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 240
           MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR
Sbjct: 181 MNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVEFR 240

Query: 241 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 300
           MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH
Sbjct: 241 MVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQRH 300

Query: 301 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 360
           GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT
Sbjct: 301 GIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCLLT 360

Query: 361 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 361 GLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 405

BLAST of CmoCh11G015750 vs. NCBI nr
Match: XP_022989653.1 (uncharacterized protein LOC111486673 [Cucurbita maxima])

HSP 1 Score: 792.0 bits (2044), Expect = 2.5e-225
Identity = 401/407 (98.53%), Postives = 402/407 (98.77%), Query Frame = 0

Query: 1   MGSAQSGQVAEER--EEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP 60
           MGSAQSGQVAEER  EEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP
Sbjct: 1   MGSAQSGQVAEEREEEEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPLSP 60

Query: 61  QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE 120
           QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE
Sbjct: 61  QLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISHGE 120

Query: 121 YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC 180
           YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC
Sbjct: 121 YELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAASVC 180

Query: 181 QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQVE 240
           QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIY PETLSFIDRIQPDFAAPAGESLRQVE
Sbjct: 181 QEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYTPETLSFIDRIQPDFAAPAGESLRQVE 240

Query: 241 FRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPHRQ 300
           FRMVHFLNATILELPEKLRSD+AIRPNETLQMSHELTNSI DRDAHSLQSSPHWDLPHRQ
Sbjct: 241 FRMVHFLNATILELPEKLRSDIAIRPNETLQMSHELTNSILDRDAHSLQSSPHWDLPHRQ 300

Query: 301 RHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL 360
           RHGI RKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL
Sbjct: 301 RHGIVRKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIKCL 360

Query: 361 LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 361 LTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 407

BLAST of CmoCh11G015750 vs. NCBI nr
Match: XP_023529264.1 (uncharacterized protein LOC111792161 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.8 bits (2041), Expect = 5.6e-225
Identity = 400/409 (97.80%), Postives = 403/409 (98.53%), Query Frame = 0

Query: 1   MGSAQSGQVAEER----EEEDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPL 60
           MGSAQSGQVAEER    EEE++DGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPL
Sbjct: 1   MGSAQSGQVAEEREEEEEEEEEDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALASPL 60

Query: 61  SPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISH 120
           SPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISH
Sbjct: 61  SPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLISH 120

Query: 121 GEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAAS 180
           GEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAAS
Sbjct: 121 GEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMAAS 180

Query: 181 VCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESLRQ 240
           VCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIY PETLSFIDRIQPDFAAPAGESLRQ
Sbjct: 181 VCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYTPETLSFIDRIQPDFAAPAGESLRQ 240

Query: 241 VEFRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPH 300
           VEFRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPH
Sbjct: 241 VEFRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDLPH 300

Query: 301 RQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDAPLNIRNSSSCTTCIGIFTHSIPIK 360
           RQRHGI RKKSGKSRLQTVTTTEEHEAEDEISPRDAPL+IRNSSSCTTCIGIFTHSIPIK
Sbjct: 301 RQRHGIVRKKSGKSRLQTVTTTEEHEAEDEISPRDAPLSIRNSSSCTTCIGIFTHSIPIK 360

Query: 361 CLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           CLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL
Sbjct: 361 CLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 409

BLAST of CmoCh11G015750 vs. NCBI nr
Match: XP_038896091.1 (uncharacterized protein LOC120084344 isoform X2 [Benincasa hispida])

HSP 1 Score: 703.4 bits (1814), Expect = 1.2e-198
Identity = 363/422 (86.02%), Postives = 378/422 (89.57%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEE---------DDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHA 60
           MGSAQS Q+AEEREE+         +DDGDDNVEPA  EL+NYLVKKVLEQEPEMLPCHA
Sbjct: 1   MGSAQSVQLAEEREEDEEEEEEDDNEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60

Query: 61  LASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQ SSLGTPRLGP IKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRAR 180
           FLISHGE ELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+SSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAG 240
           SMA SVCQEMNF EEQIQ +DALTEMS GHWE CLRS+IY PETL+FIDRIQPDF+ PAG
Sbjct: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPAG 240

Query: 241 ESLRQVEFRMVHFLNATILELPEKLRSDV-AIRPNETLQMSHELTNSIHDRDAHSLQSSP 300
           ESLRQVEFRMVHFLN TILELPEKLRSDV + RPNETLQMSHELTNSIHDRD HSL  SP
Sbjct: 241 ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSL-PSP 300

Query: 301 HWDLPHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDA-------PLNIRNSSSCT 360
           HWDLPHRQRHGI R+KSGKSRLQTVTTT EHEAEDEISP DA        L+IRNSS CT
Sbjct: 301 HWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEISPHDATHQSTLNDLSIRNSSLCT 360

Query: 361 TCIGIFTHSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLR 406
           TCIGIFTHSIPIKCLL GLLECSL MLHKFCI+DSSVTVLQHSLKSGWQIKT+NDTAHLR
Sbjct: 361 TCIGIFTHSIPIKCLLAGLLECSLVMLHKFCIEDSSVTVLQHSLKSGWQIKTLNDTAHLR 420

BLAST of CmoCh11G015750 vs. TAIR 10
Match: AT3G26780.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 478.4 bits (1230), Expect = 5.7e-135
Identity = 257/416 (61.78%), Postives = 309/416 (74.28%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREE------EDDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHALAS 60
           MGS+QS Q+ +E EE      E+++ +DN    + +L+N LVKKVLEQEPEMLPCHA AS
Sbjct: 1   MGSSQSTQMEDEEEEEEETDNEEEEEEDNGRSNTRQLDNLLVKKVLEQEPEMLPCHASAS 60

Query: 61  PLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVFLI 120
           PLSPQLSSLGTPRLGP IKVWDPYNVL+PPPQ  PP +F+R  S    D+DR V EV+LI
Sbjct: 61  PLSPQLSSLGTPRLGPSIKVWDPYNVLSPPPQ--PPSIFSRIVSG---DEDRAVTEVYLI 120

Query: 121 SHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARSMA 180
           SHGE +LNL+ DL+GGRC  AALT  GKRQARALAVF KSQGVRFN+V+SSPLDRARSMA
Sbjct: 121 SHGESDLNLKPDLIGGRCHVAALTANGKRQARALAVFFKSQGVRFNSVYSSPLDRARSMA 180

Query: 181 ASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDFAAPAGESL 240
            SVCQEM+F EE +Q +DA+ EMS G WE C +++IY PETLS I+R QPDF AP+GESL
Sbjct: 181 VSVCQEMSFPEEHVQSSDAVIEMSLGDWEGCNQAEIYSPETLSLIERCQPDFTAPSGESL 240

Query: 241 RQVEFRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSLQSSPHWDL 300
           RQVEFRMV FLN T+  + EKLRSD +   N          N  H+RD     +S +WDL
Sbjct: 241 RQVEFRMVQFLNGTVSGVAEKLRSDYSSTTNH---------NETHERDGGVSLASTNWDL 300

Query: 301 PHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRDA----PLNIRNSSS-CTTCIGIF 360
            H+ R  + RKKSGKSRLQ +T    HE +D  SPR+       ++ +SSS  + C+G+F
Sbjct: 301 LHKHRPSLTRKKSGKSRLQVMT---NHEPDDGNSPREEVNHNHTDLSDSSSLISNCVGVF 360

Query: 361 THSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDTAHLRLL 406
           THS+PIKCLLTG+L CS  M HK C++DSSVTVLQHS ++GWQIK MNDTAHLRLL
Sbjct: 361 THSLPIKCLLTGILGCSPVMTHKICVEDSSVTVLQHSWRNGWQIKRMNDTAHLRLL 399

BLAST of CmoCh11G015750 vs. TAIR 10
Match: AT1G12850.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.7e-129
Identity = 253/426 (59.39%), Postives = 296/426 (69.48%), Query Frame = 0

Query: 1   MGSAQSGQVAEEREEEDDDGDDNVEPASG--------------ELENYLVKKVLEQEPEM 60
           MGS+QS Q+ +E EEE+D+ +   E                  EL+N LVKKVLEQEPEM
Sbjct: 1   MGSSQSSQLLDEEEEEEDEAESEGEEVEEEEDEAESELNNRRIELDNLLVKKVLEQEPEM 60

Query: 61  LPCHALASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDR 120
           LPCHA ASPLSPQLSSLGTPR+GP IKVWDPYNVLAPPP    PP+F+R  SS++  D  
Sbjct: 61  LPCHASASPLSPQLSSLGTPRIGPSIKVWDPYNVLAPPPP-SSPPLFSR-ISSAAEHDRS 120

Query: 121 VVMEVFLISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP 180
            V EV+ ISHGE +LNLR DL+GGRC  A LTP GKRQARALAVFL S+GVRF +VFSSP
Sbjct: 121 AVTEVYFISHGECDLNLRPDLIGGRCHVATLTPNGKRQARALAVFLNSEGVRFTSVFSSP 180

Query: 181 LDRARSMAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYKPETLSFIDRIQPDF 240
           LDRARSMA SVCQEMNF EE +Q +DA+ EMS G WE C RS+IY PETLS I+R QPDF
Sbjct: 181 LDRARSMAVSVCQEMNFPEEHLQVSDAVVEMSLGDWESCHRSEIYTPETLSLIERCQPDF 240

Query: 241 AAPAGESLRQVEFRMVHFLNATILELPEKLRSDVAIRPNETLQMSHELTNSIHDRDAHSL 300
           +AP+GESLRQVEFRMV FLN T+  L EKLRS++      T      L  SIH       
Sbjct: 241 SAPSGESLRQVEFRMVQFLNGTVSGLSEKLRSELLPSTQHTNSRGFSLATSIH------- 300

Query: 301 QSSPHWDLPHRQRHGIARKKSGKSRLQTVTTTEEHEAEDEISPRD------APLNIRNSS 360
                       R  + RKKSGKSR Q +  T +HE  +EI            +NI++SS
Sbjct: 301 ------------RPILTRKKSGKSRFQVMNATGDHEGSEEIFSNHNDEQHLGDINIKSSS 360

Query: 361 S-CTTCIGIFTHSIPIKCLLTGLLECSLAMLHKFCIDDSSVTVLQHSLKSGWQIKTMNDT 406
           S  +TCIG+FTHS+PIKCLLTG+L CS  M HK C++DSSVTVLQHS K+GWQ+K +NDT
Sbjct: 361 SQLSTCIGVFTHSLPIKCLLTGILGCSPVMTHKICVEDSSVTVLQHSWKTGWQVKRLNDT 405