CmoCh11G015030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G015030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmediator of RNA polymerase II transcription subunit 15a-like
LocationCmo_Chr11: 10666465 .. 10668587 (-)
RNA-Seq ExpressionCmoCh11G015030
SyntenyCmoCh11G015030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAAATCATTTGGGGCCTGCTCAAGGTGGAGAATCCGGTGTCGATGCTGGGGATTGGAGGTCTCAATTGCAGCCGGAATCTCGGCATCGAATTGTCGACAAGATGTATGATTACTTTCTTCTTCCATGCCATTAATACCCTATTTGAGAAATTTGTTTGCGCTTTTGCAACTTTTTGATTCATAGTTCGCTGCTTAGATTAGTTACTATGGGATAGTTTTTTGAATTAAGCTATGAACAGCAAAACCTTATTCTACCTTTTCTTAGATTTGTGTTTAACTGATGGATGCGAACTGATTCCAAAACCTTGTTGAAGATTGTTGGGAGGAATTCCATGTTGGCTAATGAAGAGGATGATCCTAAGAAATACTATCTTTATTGGTACGAGGCCTTTTGGGAAAGCCAAAGTGAAGCCATGAGAGATTATGCTAAAAGTGGACAATATCATATCGCTGTAGAGAGTCGTGATTCCTAACATGGTATCAAAGTCATGCCCTTAACTTAGCAATGTTAATAGAATCTTCAATTGTCGAACAAAGAAATTGTAAGCCTTGAAGGTATAGTCATAAGTGCTCCAGAGAAAGGAGTCCAGCCTCGTTTAAGGGTTTAAGGAGAGGTTTAAGAGGAGACTGTCTGAGGGCTACATAGACCTCTGGGGAGGCTCTATGGTATAGTAGAGAAGGATGGTTGTGTTCGAGGGCTACATAGACCTCTGGGGAGGCTCTACGGTGTAGTAGGAAAAAGGATTGTTGGGAGATTCATGATTCCTTACAAACCTAGTTTGTTAAAGTTATCCCGTTCACTTTTCTATTTCTGGTTTGAAGATATGGTAGGTTAGATTTTGTGGAGGCAGTTCCTATTTGTTTCTTCAGGTATTATGGTAGGTTAGCTTTTGTGGTGGCGGTTCCTATTTGTTTCTTCAAGTATTAACCCTCCATTGAACATAAGACGTACAATGGAGAAGGATGGTTGTGTTCGAGGGCTACATAGACCTCTGGGGAGGCTCTACGGTGTAGTAGGAAAAAGGATTGTTGGGAGATTCATGATTCCTTACAAACCTAGTTTGTTAAAGTTATCCCGTTCACTTTTCTATTTCTGGTTTGAAGATATGGTAGGTTAGATTTTGTGGAGGCAGTTCCTATTTGTTTCTTCAGGTATTATGGTAGGTTAGCTTTTGTGGTGGCGGTTCCTATTTGTTTCTTCAAGTATTAACCCTCCATTGAACATAAGACGTACAACTTATTTGAAGTAGTATGGATCCTATTGAATTAACGGTTCTAAAGTACATTCCTTCAAAATGCTCCTATATTAGTCAGAGAGGGGAACAAAACATTCCTCTTAAGGGTGTGAAAACCTCTCCTTAATAGATGCGTTTTAGAACTGCGAGGCAGACGGCGATACGTAACAGGCTAAAGCGGACAATATTTGTTAGTAGCGAGCTTGGACTGTTACAAATGGTATCAAAGTCAGACATCGAGAGGTGTGCCAACGAAGACGCTAGGCCCTCAAAGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGGGAATGAAACATTCTTCATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCGTGAGGCTGACAGTGATATGTAAGGGGCCAAAGCGGACAATATCTGCTAGCGGTGAGCTTAAGCTGTTACACTAATATTTTGAATCATGGACGTGGTTTACAGCTATAGTGTTTCGCTCCCAAACAGCTCCCCGTGCTTTTGCGTTTAATAAACTTGTCAGCCACAATCTTTTCCATTTTTATGTAGAATTGAGACATTGAAGAGGCACCTTCCTGTTTCGGGTCCTGAGGGGTTGAGTGAGGTGAGGAAAATTGCTGTAAGGTTCGAGGAAAAGGTTTACACTGTCGCTACCAGTCAGGTACTTGCTGCTCTCACTTGTATAAAGCCCTGATCTATTATGCTATCCACTAAATACAACACTGAGTACTCACCAATTGTATTTTGAACCTCTAATTTCAGTCAGATTACCTAAGGAAAATATCTCTGAGGATGCTTACTATGGAAACCAAGCCTGGGACGACCCCTGCATTACCATCAATGTCTGGGCCTATGGCTTCCAATCAACCTTAG

mRNA sequence

ATGGATTCAAATCATTTGGGGCCTGCTCAAGGTGGAGAATCCGGTGTCGATGCTGGGGATTGGAGGTCTCAATTGCAGCCGGAATCTCGGCATCGAATTGTCGACAAGATAATTGAGACATTGAAGAGGCACCTTCCTGTTTCGGGTCCTGAGGGGTTGAGTGAGGTGAGGAAAATTGCTGTAAGGTTCGAGGAAAAGGTTTACACTGTCGCTACCAGTCAGTCAGATTACCTAAGGAAAATATCTCTGAGGATGCTTACTATGGAAACCAAGCCTGGGACGACCCCTGCATTACCATCAATGTCTGGGCCTATGGCTTCCAATCAACCTTAG

Coding sequence (CDS)

ATGGATTCAAATCATTTGGGGCCTGCTCAAGGTGGAGAATCCGGTGTCGATGCTGGGGATTGGAGGTCTCAATTGCAGCCGGAATCTCGGCATCGAATTGTCGACAAGATAATTGAGACATTGAAGAGGCACCTTCCTGTTTCGGGTCCTGAGGGGTTGAGTGAGGTGAGGAAAATTGCTGTAAGGTTCGAGGAAAAGGTTTACACTGTCGCTACCAGTCAGTCAGATTACCTAAGGAAAATATCTCTGAGGATGCTTACTATGGAAACCAAGCCTGGGACGACCCCTGCATTACCATCAATGTCTGGGCCTATGGCTTCCAATCAACCTTAG

Protein sequence

MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIAVRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP
Homology
BLAST of CmoCh11G015030 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.0e-27
Identity = 60/105 (57.14%), Postives = 85/105 (80.95%), Query Frame = 0

Query: 1   MDSNHLGPA-QGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKI 60
           MD+N+  P+   GE  +D GDWR+QL P+SR +IV+KI+ETLK+HLP SGPEG++E+R+I
Sbjct: 1   MDNNNWRPSLPNGEPAMDTGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRI 60

Query: 61  AVRFEEKVYTVATSQSDYLRKISLRMLTMETK----PGTTPALPS 101
           A RFEEK+++ A +Q+DYLRKIS++MLTMETK     G++ A+P+
Sbjct: 61  AARFEEKIFSGALNQTDYLRKISMKMLTMETKSQNAAGSSAAIPA 105

BLAST of CmoCh11G015030 vs. ExPASy TrEMBL
Match: A0A6J1EIJ8 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moschata OX=3662 GN=LOC111434576 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.5e-53
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0

Query: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60
           MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA
Sbjct: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60

Query: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 111
           VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP
Sbjct: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 110

BLAST of CmoCh11G015030 vs. ExPASy TrEMBL
Match: A0A6J1JL81 (mediator of RNA polymerase II transcription subunit 15a-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111486210 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.0e-49
Identity = 104/110 (94.55%), Postives = 106/110 (96.36%), Query Frame = 0

Query: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60
           MDSNHLGP QGGESGVDAGDWRSQLQPESRHRIV+KIIETLKRHLPVS PEG SEVRKIA
Sbjct: 1   MDSNHLGPTQGGESGVDAGDWRSQLQPESRHRIVNKIIETLKRHLPVSSPEGSSEVRKIA 60

Query: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 111
           VRFEEKVYTVATSQSDYLRKISLRML+METK GTTPALPSMSGPMASNQP
Sbjct: 61  VRFEEKVYTVATSQSDYLRKISLRMLSMETKSGTTPALPSMSGPMASNQP 110

BLAST of CmoCh11G015030 vs. ExPASy TrEMBL
Match: A0A6J1JP13 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486210 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.2e-48
Identity = 100/110 (90.91%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60
           MDSNHLGP QGGESGVDAGDWRSQLQPESRHRIV+KIIETLKRH+PVSGPEGLSE+RKIA
Sbjct: 1   MDSNHLGPTQGGESGVDAGDWRSQLQPESRHRIVNKIIETLKRHIPVSGPEGLSELRKIA 60

Query: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 111
           VRFEEKVY  ATSQSDYLRKISLRMLTMETK GTT ALPSMSGP++SNQP
Sbjct: 61  VRFEEKVYNAATSQSDYLRKISLRMLTMETKSGTTTALPSMSGPVSSNQP 110

BLAST of CmoCh11G015030 vs. ExPASy TrEMBL
Match: A0A6J1JL76 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486210 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.5e-45
Identity = 96/110 (87.27%), Postives = 102/110 (92.73%), Query Frame = 0

Query: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60
           MDSNHLGPAQGGE  VDAGDWRSQL P+SRHRIV+ IIETLKRH+PVSGPEGLSE+RKIA
Sbjct: 1   MDSNHLGPAQGGEPEVDAGDWRSQLPPDSRHRIVNSIIETLKRHIPVSGPEGLSELRKIA 60

Query: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 111
           VRFEEKVY  ATSQSDYLRKISLRMLTMETK GTT ALPSMSGP++SNQP
Sbjct: 61  VRFEEKVYNAATSQSDYLRKISLRMLTMETKSGTTTALPSMSGPVSSNQP 110

BLAST of CmoCh11G015030 vs. ExPASy TrEMBL
Match: A0A6J1EI55 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moschata OX=3662 GN=LOC111434574 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.1e-43
Identity = 93/110 (84.55%), Postives = 101/110 (91.82%), Query Frame = 0

Query: 1   MDSNHLGPAQGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIA 60
           MDSNHLGPAQGGE  VDAG WRSQL P+SRHRIV+ IIETLKRH+PVSGPEGL+E+RKIA
Sbjct: 1   MDSNHLGPAQGGEPEVDAGVWRSQLPPDSRHRIVNSIIETLKRHIPVSGPEGLNELRKIA 60

Query: 61  VRFEEKVYTVATSQSDYLRKISLRMLTMETKPGTTPALPSMSGPMASNQP 111
           VRFEEKVY  ATSQSDYLRKISLRMLTMETK GTT ALPS+SGP++SNQP
Sbjct: 61  VRFEEKVYNAATSQSDYLRKISLRMLTMETKSGTTTALPSVSGPVSSNQP 110

BLAST of CmoCh11G015030 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 122.5 bits (306), Expect = 2.1e-28
Identity = 60/105 (57.14%), Postives = 85/105 (80.95%), Query Frame = 0

Query: 1   MDSNHLGPA-QGGESGVDAGDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKI 60
           MD+N+  P+   GE  +D GDWR+QL P+SR +IV+KI+ETLK+HLP SGPEG++E+R+I
Sbjct: 1   MDNNNWRPSLPNGEPAMDTGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRI 60

Query: 61  AVRFEEKVYTVATSQSDYLRKISLRMLTMETK----PGTTPALPS 101
           A RFEEK+++ A +Q+DYLRKIS++MLTMETK     G++ A+P+
Sbjct: 61  AARFEEKIFSGALNQTDYLRKISMKMLTMETKSQNAAGSSAAIPA 105

BLAST of CmoCh11G015030 vs. TAIR 10
Match: AT1G15790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 170 Blast hits to 94 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 170; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 1.0e-17
Identity = 49/103 (47.57%), Postives = 67/103 (65.05%), Query Frame = 0

Query: 19  GDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIAVRFEEKVYTVATSQSDYL 78
           GDWR+Q    SR RIV+KI+ET  + LP   PEG +E+RKIAVRFEEK++  A++Q++YL
Sbjct: 5   GDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYL 64

Query: 79  RKISLRMLTMETK-----------PGTTPALPSMSGPMASNQP 111
           R+I ++ML METK             T P +P  S P  +N+P
Sbjct: 65  RQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVP--NNEP 105

BLAST of CmoCh11G015030 vs. TAIR 10
Match: AT1G15790.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 1.0e-17
Identity = 49/103 (47.57%), Postives = 67/103 (65.05%), Query Frame = 0

Query: 19  GDWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIAVRFEEKVYTVATSQSDYL 78
           GDWR+Q    SR RIV+KI+ET  + LP   PEG +E+RKIAVRFEEK++  A++Q++YL
Sbjct: 5   GDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYL 64

Query: 79  RKISLRMLTMETK-----------PGTTPALPSMSGPMASNQP 111
           R+I ++ML METK             T P +P  S P  +N+P
Sbjct: 65  RQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVP--NNEP 105

BLAST of CmoCh11G015030 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 50.8 bits (120), Expect = 7.9e-07
Identity = 26/69 (37.68%), Postives = 41/69 (59.42%), Query Frame = 0

Query: 20 DWRSQLQPESRHRIVDKIIETLKRHLPVS------GPEGLSEVRKIAVRFEEKVYTVATS 79
          DWRSQ +PE R +++ KI+ +L  +  V             ++  IA +FEE  Y++AT 
Sbjct: 24 DWRSQHEPELRQKVLSKIVCSLNDYRKVEKFKEKFHAHEEYKINDIASKFEENFYSIATD 83

Query: 80 QSDYLRKIS 83
          ++DYLRK+S
Sbjct: 84 KNDYLRKLS 92

BLAST of CmoCh11G015030 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 42.0 bits (97), Expect = 3.7e-04
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 0

Query: 20 DWRSQLQPESRHRIVDKIIETLKRHLPVSGPEGLSEVRKIAVRFEEKVYTVATSQS 76
          DWRSQ +PE R +++ KI+E  K            ++  IA +FEE  Y++AT ++
Sbjct: 24 DWRSQHEPELRQKVLSKIVEKFKEKFHAHEE---YKINDIASKFEENFYSIATDKT 76

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I1713.0e-2757.14Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana ... [more]
Match NameE-valueIdentityDescription
A0A6J1EIJ81.5e-53100.00mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moscha... [more]
A0A6J1JL811.0e-4994.55mediator of RNA polymerase II transcription subunit 15a-like isoform X3 OS=Cucur... [more]
A0A6J1JP133.2e-4890.91mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Cucur... [more]
A0A6J1JL761.5e-4587.27mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Cucur... [more]
A0A6J1EI551.1e-4384.55mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
AT1G15780.12.1e-2857.14unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G15790.11.0e-1747.57unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G15790.21.0e-1747.57unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G10440.27.9e-0737.68unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G10440.13.7e-0435.71unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036546Mediator complex subunit 15, KIX domainPFAMPF16987KIX_2coord: 18..92
e-value: 1.9E-34
score: 117.5
IPR036529Coactivator CBP, KIX domain superfamilyGENE3D1.10.246.20Coactivator CBP, KIX domaincoord: 20..96
e-value: 2.9E-21
score: 77.3
IPR036529Coactivator CBP, KIX domain superfamilySUPERFAMILY47040Kix domain of CBP (creb binding protein)coord: 19..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..110
NoneNo IPR availablePANTHERPTHR33137:SF27OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A, PUTATIVE-RELATEDcoord: 1..97
IPR044661Mediator of RNA polymerase II transcription subunit 15a/b/c-likePANTHERPTHR33137MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATEDcoord: 1..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G015030.1CmoCh11G015030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0031490 chromatin DNA binding
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0003712 transcription coregulator activity