CmoCh11G012460 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G012460
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr11: 7830333 .. 7832235 (-)
RNA-Seq ExpressionCmoCh11G012460
SyntenyCmoCh11G012460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGTAATTCGTATTCTCCACTTTCAGCATAAGCTCTCGGTTGATTAAGAAGTTGATGGGTTATTGTTTCTTTTATTAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGGTGGGTTAATAATGCATTTTTTTAGAACATAAGGATGGACGACGTGGATCAATGATTTGTTGTTGTTGTCGTCGTTGTTGTTTGCAGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA

mRNA sequence

ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA

Coding sequence (CDS)

ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA

Protein sequence

MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDFPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKAGVGN
Homology
BLAST of CmoCh11G012460 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 2.0e-186
Identity = 318/572 (55.59%), Postives = 425/572 (74.30%), Query Frame = 0

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMDY++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E +KKALKT+++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      ++FP
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W+D   +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+   KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           ++ +  + FTP ++I GD WI   GVPY+ G+
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh11G012460 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 641.3 bits (1653), Expect = 1.0e-182
Identity = 317/574 (55.23%), Postives = 425/574 (74.04%), Query Frame = 0

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  ++ ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDD-ESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK  +DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMDY++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E MKKALKT++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E FP
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            WVD   ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 542
           RPWKEYSRTIIM ++I D + P+GW PW GD+GL+T FY+E  N GPGS  A RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           K+++ +  + FTP ++I GD WI   GVPY +G+
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh11G012460 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.3e-149
Identity = 269/569 (47.28%), Postives = 378/569 (66.43%), Query Frame = 0

Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIV----PVQIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKK-SVTL-RQIEKDPRASQALAV 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK SV L  +   D     AL +
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  T    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDFPE 250
           + M+K  KTS  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           WV P  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYIKQGVYN 308

Query: 311 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFENT 370
           E VD+ KK+TH+  IGDGP KT ITG+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 550
           WKE+SRTIIM + IDD+IDP GWLPW GD+ L T +Y E+ N GPGS +A+RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITPQHAVDFTPGRFILGDRWIKATGVPYV 568
           ++P+ A+ FTP RF+ G+ WI    VPY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CmoCh11G012460 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 505.4 bits (1300), Expect = 8.7e-142
Identity = 258/575 (44.87%), Postives = 367/575 (63.83%), Query Frame = 0

Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGD 188
            +C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDF 248
             + M K  KTS  LSSN LAMVT +S+++    + G+        R+LLS      +  
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----EDSI 245

Query: 249 PEWVDPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIK 308
           P WV P  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK
Sbjct: 246 PTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIK 305

Query: 309 QGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNF-VDGTNTFKTATVAVSAEHFMARDI 368
           +G+YKE V + KK+ H+  IGDGP KT+ITG+ NF +    TF TAT+ +  +HF A++I
Sbjct: 306 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 365

Query: 369 GFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFG 428
           G ENTAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FG
Sbjct: 366 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 425

Query: 429 DAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRS 488
           DA  + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++
Sbjct: 426 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 485

Query: 489 YLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKW 548
           YLGRPWKE+SRTIIM++ IDD+IDP GWLPW+GD+ L+T +Y E  N GPGS +A+RVKW
Sbjct: 486 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 545

Query: 549 RGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVS 569
            GIK +TPQ A+ +T  RF+ GD WI  T VPY +
Sbjct: 546 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CmoCh11G012460 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 1.1e-131
Identity = 271/703 (38.55%), Postives = 377/703 (53.63%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSM---KAIKAICRPTD 72
           +Y ++GV++ L++AMV+ V        DG S     ++ H  S  M     +  IC  TD
Sbjct: 3   KYVLLGVTA-LIMAMVICVEA-----NDGNSPSRKMEEVHRESKLMITKTTVSIICASTD 62

Query: 73  YKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQALAVC 132
           YK++C  SL      DP+ L++  F  A+  I++   + +     + + D    +AL  C
Sbjct: 63  YKQDCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTC 122

Query: 133 KELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDASE 192
           +ELMD ++D+L+ +  K   F  ++L + + D+ +WLS  ITYQ+TC+DGF     +A+ 
Sbjct: 123 RELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 193 KMKKALKTSMRLSSNGLAMVTELSSMLTELQI--PGINRRLLSIPVLG------------ 252
            M++ ++    L+SNGLA+   L  +L   +I  P +  R   + +LG            
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 253 -----HED---------------------------------------------------- 312
                +ED                                                    
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 373 ----------FPEWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRK 432
                     FP WV P  +RLLA  P     K N+VVAKDGSG+ KTI +A+  VP  K
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MK 422

Query: 433 NNATFVIHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFV-DGTNTFKTATVAV 492
           N   FVIHIK+GVYKE V++ KK+ H+M +GDGP KT+ITG+  F+ D   T++TA+VAV
Sbjct: 423 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 482

Query: 493 SAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCT 552
           + ++FMA+DIGFENTAG  +HQAVALRV AD  VFFNC M+GYQDTLYVHT RQFYR+C 
Sbjct: 483 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 542

Query: 553 ISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDAD 567
           +SGTIDFVFGDA AVFQNC F++R+P+ +QQ IVTA GRK+RR+ + ++I NS+   DA 
Sbjct: 543 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 602

BLAST of CmoCh11G012460 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA 120
           IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA 120

Query: 121 SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180
           SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN
Sbjct: 121 SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDFPEW 240
           TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDFPEW
Sbjct: 181 TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDFPEW 240

Query: 241 VDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE 300
           VDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE
Sbjct: 241 VDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE 300

Query: 301 YVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAG 360
           YVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAG
Sbjct: 301 YVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAG 360

Query: 361 PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ 420
           PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ
Sbjct: 361 PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ 420

Query: 421 NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK 480
           NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK
Sbjct: 421 NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK 480

Query: 481 EYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSIT 540
           EYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSIT
Sbjct: 481 EYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSIT 540

Query: 541 PQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKAGVGN 580
           PQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKAGVGN
Sbjct: 541 PQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKAGVGN 579

BLAST of CmoCh11G012460 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 558/579 (96.37%), Postives = 567/579 (97.93%), Query Frame = 0

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKK HEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA 120
           IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKS+TLRQIEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPRA 120

Query: 121 SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180
           SQALA CKELMD+SVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN
Sbjct: 121 SQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDFPEW 240
           +TGDASEKMKKALK SMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHED+PEW
Sbjct: 181 STGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDYPEW 240

Query: 241 VDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE 300
             PGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE
Sbjct: 241 AGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKE 300

Query: 301 YVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAG 360
           YV I KKLTHLMLIGDGPKKTII+GNKNFVDGT TFKTATVAVSAEHFMARDIGFENTAG
Sbjct: 301 YVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENTAG 360

Query: 361 PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ 420
           PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ
Sbjct: 361 PHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQ 420

Query: 421 NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK 480
           NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK
Sbjct: 421 NCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWK 480

Query: 481 EYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSIT 540
           EYSRTIIMESYIDDII+PEGWLPW GDWGLRTCFYTEFNN+GPGSGK+KRVKWRGIKSIT
Sbjct: 481 EYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKSIT 540

Query: 541 PQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKAGVGN 580
            QHAVDFTPGRFILGDRWIKATG+PYVSGMTKP AGV N
Sbjct: 541 AQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVAN 579

BLAST of CmoCh11G012460 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 5.3e-283
Identity = 479/575 (83.30%), Postives = 534/575 (92.87%), Query Frame = 0

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KSV L Q+EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           RAS+ALA CKELMD+++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240
           ANTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSML+ELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EYV ++K LTHLMLIGDGPKKTIITGNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGLRTCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHAVDFTPGRF+ GDRWIK TGVPYVSG+T+
Sbjct: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574

BLAST of CmoCh11G012460 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.3e-277
Identity = 466/575 (81.04%), Postives = 527/575 (91.65%), Query Frame = 0

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+EC+ SLK  G+  SDP+ELVQ GFKAAMKLIQAAA KSV L+Q+EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           R S+ALA CKELMD+++DELK+S+ KLG FDI+KLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV++ SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   ++ KPN+VVAKDGSGQ++TIQ A+++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EY+ I+K +THLMLIGDGPKKTIITGNKNFVDGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN   K D DL PV+KQFRS+LGR
Sbjct: 421 IFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHA+DFT GRF++GDRWIK TGVPYVSGMT+
Sbjct: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 574

BLAST of CmoCh11G012460 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.3e-277
Identity = 466/575 (81.04%), Postives = 527/575 (91.65%), Query Frame = 0

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+EC+ SLK  G+  SDP+ELVQ GFKAAMKLIQAAA KSV L+Q+EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           R S+ALA CKELMD+++DELK+S+ KLG FDI+KLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV++ SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   ++ KPN+VVAKDGSGQ++TIQ A+++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EY+ I+K +THLMLIGDGPKKTIITGNKNFVDGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN   K D DL PV+KQFRS+LGR
Sbjct: 421 IFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHA+DFT GRF++GDRWIK TGVPYVSGMT+
Sbjct: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 574

BLAST of CmoCh11G012460 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 653.7 bits (1685), Expect = 1.4e-187
Identity = 318/572 (55.59%), Postives = 425/572 (74.30%), Query Frame = 0

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMDY++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E +KKALKT+++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      ++FP
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W+D   +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+   KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           ++ +  + FTP ++I GD WI   GVPY+ G+
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh11G012460 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 641.3 bits (1653), Expect = 7.2e-184
Identity = 317/574 (55.23%), Postives = 425/574 (74.04%), Query Frame = 0

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  ++ ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDD-ESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK  +DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMDY++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E MKKALKT++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E FP
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            WVD   ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 542
           RPWKEYSRTIIM ++I D + P+GW PW GD+GL+T FY+E  N GPGS  A RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           K+++ +  + FTP ++I GD WI   GVPY +G+
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh11G012460 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 530.0 bits (1364), Expect = 2.4e-150
Identity = 269/569 (47.28%), Postives = 378/569 (66.43%), Query Frame = 0

Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIV----PVQIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKK-SVTL-RQIEKDPRASQALAV 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK SV L  +   D     AL +
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  T    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDFPE 250
           + M+K  KTS  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           WV P  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYIKQGVYN 308

Query: 311 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFENT 370
           E VD+ KK+TH+  IGDGP KT ITG+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 550
           WKE+SRTIIM + IDD+IDP GWLPW GD+ L T +Y E+ N GPGS +A+RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITPQHAVDFTPGRFILGDRWIKATGVPYV 568
           ++P+ A+ FTP RF+ G+ WI    VPY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CmoCh11G012460 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 505.4 bits (1300), Expect = 6.2e-143
Identity = 258/575 (44.87%), Postives = 367/575 (63.83%), Query Frame = 0

Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGD 188
            +C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDF 248
             + M K  KTS  LSSN LAMVT +S+++    + G+        R+LLS      +  
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----EDSI 245

Query: 249 PEWVDPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIK 308
           P WV P  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK
Sbjct: 246 PTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIK 305

Query: 309 QGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNF-VDGTNTFKTATVAVSAEHFMARDI 368
           +G+YKE V + KK+ H+  IGDGP KT+ITG+ NF +    TF TAT+ +  +HF A++I
Sbjct: 306 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 365

Query: 369 GFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFG 428
           G ENTAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FG
Sbjct: 366 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 425

Query: 429 DAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRS 488
           DA  + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++
Sbjct: 426 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 485

Query: 489 YLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKW 548
           YLGRPWKE+SRTIIM++ IDD+IDP GWLPW+GD+ L+T +Y E  N GPGS +A+RVKW
Sbjct: 486 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 545

Query: 549 RGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVS 569
            GIK +TPQ A+ +T  RF+ GD WI  T VPY +
Sbjct: 546 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CmoCh11G012460 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 471.9 bits (1213), Expect = 7.6e-133
Identity = 271/703 (38.55%), Postives = 377/703 (53.63%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSM---KAIKAICRPTD 72
           +Y ++GV++ L++AMV+ V        DG S     ++ H  S  M     +  IC  TD
Sbjct: 3   KYVLLGVTA-LIMAMVICVEA-----NDGNSPSRKMEEVHRESKLMITKTTVSIICASTD 62

Query: 73  YKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQALAVC 132
           YK++C  SL      DP+ L++  F  A+  I++   + +     + + D    +AL  C
Sbjct: 63  YKQDCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTC 122

Query: 133 KELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDASE 192
           +ELMD ++D+L+ +  K   F  ++L + + D+ +WLS  ITYQ+TC+DGF     +A+ 
Sbjct: 123 RELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 193 KMKKALKTSMRLSSNGLAMVTELSSMLTELQI--PGINRRLLSIPVLG------------ 252
            M++ ++    L+SNGLA+   L  +L   +I  P +  R   + +LG            
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 253 -----HED---------------------------------------------------- 312
                +ED                                                    
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 373 ----------FPEWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRK 432
                     FP WV P  +RLLA  P     K N+VVAKDGSG+ KTI +A+  VP  K
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MK 422

Query: 433 NNATFVIHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFV-DGTNTFKTATVAV 492
           N   FVIHIK+GVYKE V++ KK+ H+M +GDGP KT+ITG+  F+ D   T++TA+VAV
Sbjct: 423 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 482

Query: 493 SAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCT 552
           + ++FMA+DIGFENTAG  +HQAVALRV AD  VFFNC M+GYQDTLYVHT RQFYR+C 
Sbjct: 483 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 542

Query: 553 ISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDAD 567
           +SGTIDFVFGDA AVFQNC F++R+P+ +QQ IVTA GRK+RR+ + ++I NS+   DA 
Sbjct: 543 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E8Z82.0e-18655.59Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX861.0e-18255.23Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ213.3e-14947.28Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA18.7e-14244.87Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
O234471.1e-13138.55Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1F0580.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1[more]
A0A6J1HWF10.0e+0096.37Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1[more]
A0A0A0LYF55.3e-28383.30Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1[more]
A0A5D3CGC33.3e-27781.04Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180... [more]
A0A1S3BPI83.3e-27781.04Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.11.4e-18755.59Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.17.2e-18455.23Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.12.4e-15047.28Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.16.2e-14344.87Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.17.6e-13338.55Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 56..206
e-value: 6.6E-37
score: 138.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 60..206
e-value: 3.4E-25
score: 89.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 57..210
e-value: 2.9E-23
score: 80.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 56..211
e-value: 1.6E-33
score: 117.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 59..211
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 259..556
e-value: 5.6E-140
score: 465.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 253..570
e-value: 3.1E-118
score: 396.8
NoneNo IPR availablePANTHERPTHR31707:SF131PECTINESTERASEcoord: 16..572
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 16..572
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 63..212
e-value: 4.2984E-42
score: 146.814
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 404..413
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 257..569

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G012460.1CmoCh11G012460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity