Homology
BLAST of CmoCh11G010030 vs. ExPASy Swiss-Prot
Match:
O74343 (Multisubstrate pseudouridine synthase 7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pus7 PE=3 SV=1)
HSP 1 Score: 339.0 bits (868), Expect = 1.3e-91
Identity = 238/698 (34.10%), Postives = 353/698 (50.57%), Query Frame = 0
Query: 45 ESDVGILSYIS-SLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSE------ 104
ESDVGI ++++ +LP GI+K R++DF V EVDTDGN+VHLT ++A I+S+
Sbjct: 49 ESDVGIDAFLNPNLPSIDGIIKARFTDFAVFEVDTDGNIVHLTDMEAHDPILSKATGDKE 108
Query: 105 ------------SKPMKVHGTSESYASEI-----ELFRSLAGASDAEILEAFLKQISSGV 164
S K E + + +L + E +++ K +
Sbjct: 109 TEDAKDSSNQDISNDQKAPSFKEQEPATLPILPNDLQSIIPKGIGNEFIQSLHKLSVGEI 168
Query: 165 DDSISPIV--FAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPDSSSKCVRVRVDSGLQNNR 224
D IS I+ P DK RT +H F + NF G +SS+K
Sbjct: 169 TDPISLILPENTPPMDKGQRTILHQFIRN-NF------SGLESSTK------------GN 228
Query: 225 GRVSKKRKERGDKPFDSRGSD-NWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRS 284
G + + R ++P R +W G++ FHLYKEN+D+ + LG I ++L + TR+
Sbjct: 229 GTFTVSKTTRKNQPRSRRDPRLSWKALGGEYCHFHLYKENRDSMDCLGKIARLLKVPTRT 288
Query: 285 FGFAGTKDKRAITTQRVTVFKQHASRLAALN-----DRLIGIKLGNFSYIQEGLLLGQLL 344
AGTKD+R +T QRV + ASRLA LN + G LGN+SY L LG L
Sbjct: 289 LSIAGTKDRRGVTCQRVAIHHVRASRLAQLNSGSLKNSTYGFLLGNYSYKNSNLRLGDLK 348
Query: 345 GNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWK 404
GN F I +R V+ E ++A +L HGFINYFGLQRFG+ SV TH +G LL+ +WK
Sbjct: 349 GNEFHIVVRNVITPKEKVVEA-LNSLKEHGFINYFGLQRFGTSSVGTHTIGVRLLQSDWK 408
Query: 405 GAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAILQCLKKCPG--NY 464
GAV++IL PR T + EA + + ++D + +L+ LPR ++AE +IL+ + +Y
Sbjct: 409 GAVDLILSPRPEHTGSVKEAIDLWHSTHDAEASLRILPRRMIAESSILETWSRSGNQTDY 468
Query: 465 LQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQI 524
L A + IPR LR +Y H+YQSY+WN AS R++ G D+ VVGDLVY E++ + I
Sbjct: 469 LGAFQRIPRHLRSIYPHAYQSYVWNRVASWRIKNLG-DRPVVGDLVYSTESNGLSQKSPI 528
Query: 525 SEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPG 584
VD + D LE DLP + + ++V NF+I D+VLPLPG
Sbjct: 529 ----VDPEAPDL-------LE-----DLPVSSKLSARPIEEDEV--NNFSIYDIVLPLPG 588
Query: 585 SRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELIS 644
VIYP N+ ++Y + + S++ +E S + G YR + RP++ E+ I
Sbjct: 589 RNVIYPKNETFDIYKSVMNEASLDPLNMSRKDRELS---LPGDYRKLLVRPENMEFNFIK 648
Query: 645 YVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANEN 704
Y + L +D D L+++S+ ++ E H
Sbjct: 649 YDNMEQQLILTDKDR-LENRSISVSSEVGKH----------------------------- 664
Query: 705 ENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTS 709
A+ L F LP+S+YATMA+RE L+T+T+
Sbjct: 709 ----------TAVTLKFVLPSSAYATMALREALRTATA 664
BLAST of CmoCh11G010030 vs. ExPASy Swiss-Prot
Match:
Q08647 (Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PUS7 PE=1 SV=1)
HSP 1 Score: 338.6 bits (867), Expect = 1.8e-91
Identity = 239/687 (34.79%), Postives = 362/687 (52.69%), Query Frame = 0
Query: 43 IGESDVGILSYIS-SLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPM 102
I E+DVGI ++S LPGFRG +KQRY+DF+VNE+D +G V+HLT +
Sbjct: 38 IHEADVGITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKE 97
Query: 103 KVHGTSESYASEIELFRSLAGASDAEILEAF----LKQISSGVDDSISPIVFAPDTDKSH 162
+V+ ES A+ + F ++ +++E F + +I S + DKS
Sbjct: 98 EVNAEKESEAARRQEF-NVDPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSV 157
Query: 163 RTAVHNFFKKFNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRG 222
RT +H ++ ++V ++ K R N R +K +E+ ++ D+ G
Sbjct: 158 RTKIHQLLREAFKNELESVTTDTNTFKIAR-------SNRNSRTNK--QEKINQTRDANG 217
Query: 223 SDNWPEHVGK-FLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTV 282
+NW K F+ F L+KENKDT EA+ +I K+L + +R +AGTKD+RA+T QRV++
Sbjct: 218 VENWGYGPSKDFIHFTLHKENKDTMEAVNVITKLLRVPSRVIRYAGTKDRRAVTCQRVSI 277
Query: 283 FKQHASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVVADS-----EDTI 342
K RL ALN L G+ +GN+++ L LG L GN F + +R V + E+ +
Sbjct: 278 SKIGLDRLNALNRTLKGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTGNSEVSLEEIV 337
Query: 343 KASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNVIAE 402
++L +GFINYFG+QRFG+ S+ TH +G LL WK A +IL ++ E
Sbjct: 338 SNGCKSLSENGFINYFGMQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKE 397
Query: 403 ARNYYKESNDIDGTLKQLPRYLVAERAILQCL----KKCPGNYLQ-----ALKAIPRTLR 462
AR + E+ D LKQ+PR +AE A+L L K+ G Y + A+ IPR LR
Sbjct: 398 ARKIWAETKDAALALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLR 457
Query: 463 MMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQISEENVDEDCGDA 522
MYVH+YQSY+WN AS R++ +G+ K+VVGDLV + + I +E+ DED +A
Sbjct: 458 TMYVHAYQSYVWNSIASKRIELHGL-KLVVGDLV-IDTSEKSPLISGIDDEDFDEDVREA 517
Query: 523 NTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRVIYPTN-DIA 582
+ KAVT ED+ S +T++DVVLP PG V+YP+N ++
Sbjct: 518 QFIRA-------------------KAVTQEDIDSVKYTMEDVVLPSPGFDVLYPSNEELK 577
Query: 583 EVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAES 642
++Y D+ D+++ V++FS + GSYR+V Q+PK E+ +I Y D + L +
Sbjct: 578 QLYVDILKADNMDPFNMRRKVRDFS---LAGSYRTVIQKPKSLEYRIIHYDDPSQQLVNT 637
Query: 643 DLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQM 702
DLD L +++ ++SG KL + G +
Sbjct: 638 DLDILNNTRA----------------------KESGQKYMKAKLDRYMPDKG----GEKT 664
Query: 703 ALKLSFNLPTSSYATMAIRELLKTSTS 709
A+ L F L TS+YATMA+REL+K TS
Sbjct: 698 AVVLKFQLGTSAYATMALRELMKLETS 664
BLAST of CmoCh11G010030 vs. ExPASy Swiss-Prot
Match:
Q96PZ0 (Pseudouridylate synthase 7 homolog OS=Homo sapiens OX=9606 GN=PUS7 PE=1 SV=2)
HSP 1 Score: 333.2 bits (853), Expect = 7.4e-90
Identity = 228/694 (32.85%), Postives = 334/694 (48.13%), Query Frame = 0
Query: 32 SSSITVMAMKSIGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAP 91
S S M + E+DVGI ++SS GF GILK+RYSDF+V+E+ DG + HL L P
Sbjct: 98 SESFADMMKHGLTEADVGITKFVSSHQGFSGILKERYSDFVVHEIGKDGRISHLNDLSIP 157
Query: 92 PEIVSESKPMKVHGTSE--SYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISPIVF 151
+ S+ + T+E E++LF++ + E++E
Sbjct: 158 VDEEDPSEDIFTVLTAEEKQRLEELQLFKNKETSVAIEVIE------------------- 217
Query: 152 APDTDKSHRTAVHNFFKK-FNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKK---- 211
DT K RT +H K F L T T D R KK
Sbjct: 218 --DT-KEKRTIIHQAIKSLFPGLETKTED----------------------REGKKYIVA 277
Query: 212 RKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTK 271
G K + +WP+ G + F LYKENKDT +A+ ++ K L ++ F + GTK
Sbjct: 278 YHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTK 337
Query: 272 DKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVV 331
DKRAIT Q + V K A RLA LN L+ KLGNFSY + L LG+L GN FT+ LR +
Sbjct: 338 DKRAITVQEIAVLKITAQRLAHLNKCLMNFKLGNFSYQKNPLKLGELQGNHFTVVLRNIT 397
Query: 332 ADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG 391
++D ++ + +L GFINY+G+QRFG+ +VPT+ VG ++L+ W +++IL PR G
Sbjct: 398 G-TDDQVQQAMNSLKEIGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSG 457
Query: 392 -ETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAILQCLKKC-PGNYLQALKAIPRTLR 451
E + + R + ++ D L++LP E +L+ L K N + A IPR R
Sbjct: 458 AEKGYLVKCREEWAKTKDPTAALRKLPVKRCVEGQLLRGLSKYGMKNIVSAFGIIPRNNR 517
Query: 452 MMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQISEENVDEDCGDA 511
+MY+HSYQSY+WN+ S R++ YG+ K V GDLV A I E++V+
Sbjct: 518 LMYIHSYQSYVWNNMVSKRIEDYGL-KPVPGDLVL-----KGATATYIEEDDVN------ 577
Query: 512 NTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAE 571
N++I DVV+PLPG VIYP + I E
Sbjct: 578 -----------------------------------NYSIHDVVMPLPGFDVIYPKHKIQE 637
Query: 572 VYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESD 631
Y ++ D++++ H ++++S ++G+YR + RP++ WE+++Y D +PL +D
Sbjct: 638 AYREMLTADNLDIDNMRHKIRDYS---LSGAYRKIIIRPQNVSWEVVAYDDPKIPLFNTD 656
Query: 632 LDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMA 691
+DNL A E K A
Sbjct: 698 VDNLEGKTPPVFASEGKYR----------------------------------------A 656
Query: 692 LKLSFNLPTSSYATMAIRELLKTSTSVAFHKALN 717
LK+ F+LP S+YATMAIRE+LK TS+ LN
Sbjct: 758 LKMDFSLPPSTYATMAIREVLKMDTSIKNQTQLN 656
BLAST of CmoCh11G010030 vs. ExPASy Swiss-Prot
Match:
Q08DI8 (Pseudouridylate synthase 7 homolog OS=Bos taurus OX=9913 GN=PUS7 PE=2 SV=1)
HSP 1 Score: 330.9 bits (847), Expect = 3.7e-89
Identity = 231/694 (33.29%), Postives = 338/694 (48.70%), Query Frame = 0
Query: 32 SSSITVMAMKSIGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAP 91
+ S M + E DVGI ++SS GF GILK+RYSDF+V+E+ DG + HL+ L P
Sbjct: 96 AESFADMMKHGLTELDVGITKFVSSHQGFSGILKERYSDFVVHEIGRDGRISHLSDLSVP 155
Query: 92 PEIVSESKPMKVHGTSE--SYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISPIVF 151
+ S+ + T+E E++LF++ + E++E
Sbjct: 156 VDEEDPSEDVFTVLTAEEKQRLEELQLFKNKETSVAIEVIE------------------- 215
Query: 152 APDTDKSHRTAVHNFFKK-FNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKK---- 211
DT K RT +H K F L T T D R KK
Sbjct: 216 --DT-KEKRTIIHQAIKSLFPGLETKTED----------------------REGKKYIVA 275
Query: 212 RKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTK 271
G K + +WP+ G + F LYKENKDT +A+ L+ K L ++ F + GTK
Sbjct: 276 YHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINLLSKYLRVKPNIFSYMGTK 335
Query: 272 DKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVV 331
DKRAIT Q + V K A RLA LN L+ KLGNFSY + L LG+L GN FT+ LR +
Sbjct: 336 DKRAITVQEIAVLKITAQRLAHLNKCLMNFKLGNFSYQKNPLKLGELQGNHFTVVLRNIT 395
Query: 332 ADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG 391
+++ ++ + +L GFINY+G+QRFG+ +VPT+ VG ++L+ W +++IL PR G
Sbjct: 396 G-TDNQVEQAMNSLKEIGFINYYGMQRFGTTAVPTYQVGRAILQNSWAEVMDLILKPRSG 455
Query: 392 -ETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAILQCLKKC-PGNYLQALKAIPRTLR 451
E + + R + +S D L++LP E +L+ L K N + A IPR R
Sbjct: 456 AEKGYLVKCREEWAKSKDPAAALRKLPVKRCVEGQLLRGLSKYGMKNIVSAFGIIPRNNR 515
Query: 452 MMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQISEENVDEDCGDA 511
+MY+HSYQSY+WN+ S R+ +YG+ K V GDLV + TAT ++ED D
Sbjct: 516 LMYIHSYQSYVWNNMVSKRIDEYGL-KPVPGDLVL--KGATATF--------IEED--DV 575
Query: 512 NTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAE 571
N N++I DVV+PLPG VIYP + I+E
Sbjct: 576 N----------------------------------NYSIHDVVMPLPGFDVIYPKHKISE 635
Query: 572 VYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESD 631
Y ++ D++++ H ++++S ++G+YR + RP++ WE+++Y D +PL +D
Sbjct: 636 AYREMLTADNLDIDNMRHKIRDYS---LSGAYRKIIIRPQNVSWEVVAYDDPKIPLFNTD 654
Query: 632 LDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMA 691
+DNL A E K A
Sbjct: 696 VDNLEGRPPPVFASEGKYR----------------------------------------A 654
Query: 692 LKLSFNLPTSSYATMAIRELLKTSTSVAFHKALN 717
LK+ F+LP S+YATMAIRE+LK TS+ LN
Sbjct: 756 LKMDFSLPPSTYATMAIREVLKMDTSIKNQTQLN 654
BLAST of CmoCh11G010030 vs. ExPASy Swiss-Prot
Match:
Q91VU7 (Pseudouridylate synthase 7 homolog OS=Mus musculus OX=10090 GN=Pus7 PE=2 SV=2)
HSP 1 Score: 326.6 bits (836), Expect = 6.9e-88
Identity = 223/693 (32.18%), Postives = 330/693 (47.62%), Query Frame = 0
Query: 32 SSSITVMAMKSIGESDVGILSYISSLPGFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAP 91
+ S M + E DVGI ++SS GF GILK+RYSDF+V+E+ DG + HL L P
Sbjct: 97 AESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVP 156
Query: 92 PEIVSESKPMK-----VHGTSESYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISP 151
V E P + + E++LF++ + E++E
Sbjct: 157 ---VDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIE---------------- 216
Query: 152 IVFAPDTDKSHRTAVHNFFKK-FNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKKR 211
DT K RT +H K F L T T D +
Sbjct: 217 -----DT-KEKRTVIHQAIKSLFPGLETKTED------------------REGRKYIVAY 276
Query: 212 KERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTKD 271
G K + +WP+ G + F LYKENKDT +A+ ++ K L ++ F + GTKD
Sbjct: 277 HAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKD 336
Query: 272 KRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVVA 331
KRAIT Q + V K A RLA LN L+ +KLGNFSY + L LG L GN FT+ LR +
Sbjct: 337 KRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITG 396
Query: 332 DSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREG- 391
+++ ++ + ++L GFINY+G+QRFG+ +VPT+ VG ++L+ W +++IL PR G
Sbjct: 397 -TDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGA 456
Query: 392 ETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAILQCLKKC-PGNYLQALKAIPRTLRM 451
E + + R + ++ D LK+LP E +L+ L + N + A IPR R+
Sbjct: 457 EKGYLVKCREEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRL 516
Query: 452 MYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQISEENVDEDCGDAN 511
MY+HSYQSY+WN S R+++YG+ + V GDLV I E++VD
Sbjct: 517 MYIHSYQSYVWNTMVSRRIEEYGL-RPVPGDLVL-----KGATPTYIEEDDVD------- 576
Query: 512 TYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEV 571
N++I DVV+PLPG VIYP + I+E
Sbjct: 577 ----------------------------------NYSIHDVVMPLPGFDVIYPKHKISEA 636
Query: 572 YHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESDL 631
Y ++ A D++++ H ++++S ++G+YR + RP+ WE+++Y D +PL +D+
Sbjct: 637 YREMLAADNLDIDNMRHTIRDYS---LSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDV 655
Query: 632 DNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMAL 691
DNL A E K AL
Sbjct: 697 DNLEGKPPPVFASEGKYR----------------------------------------AL 655
Query: 692 KLSFNLPTSSYATMAIRELLKTSTSVAFHKALN 717
K+ F+LP S+YATMAIRE+LK TS+ LN
Sbjct: 757 KMDFSLPPSTYATMAIREVLKMDTSIKNQTQLN 655
BLAST of CmoCh11G010030 vs. ExPASy TrEMBL
Match:
A0A6J1F560 (multisubstrate pseudouridine synthase 7 OS=Cucurbita moschata OX=3662 GN=LOC111440943 PE=3 SV=1)
HSP 1 Score: 1413.3 bits (3657), Expect = 0.0e+00
Identity = 717/717 (100.00%), Postives = 717/717 (100.00%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT
Sbjct: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE
Sbjct: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 717
BLAST of CmoCh11G010030 vs. ExPASy TrEMBL
Match:
A0A6J1KMR0 (multisubstrate pseudouridine synthase 7 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496015 PE=3 SV=1)
HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 707/717 (98.61%), Postives = 709/717 (98.88%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPY LCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYFLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMK HGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKGHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVF+PDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFSPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIK SAEALGR GFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKTSAEALGRLGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPTERNK VKAV+AEDVQSGNFT
Sbjct: 481 NHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPTERNKHVKAVSAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKS SVEI KEEKMHDKSKDEDSLDSTEQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSLSVEITKEEKMHDKSKDEDSLDSTEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE
Sbjct: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 717
BLAST of CmoCh11G010030 vs. ExPASy TrEMBL
Match:
A0A6J1DY30 (pseudouridylate synthase 7 homolog OS=Momordica charantia OX=3673 GN=LOC111025594 PE=3 SV=1)
HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 625/720 (86.81%), Postives = 667/720 (92.64%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTI--TKCTNRSSSITVMAMKSIGESDVGILSYISSLP 60
M WHRPG +Y CALKPY LC FT TKCTN+SSSITVMA K+IGESDVGI+SYIS+LP
Sbjct: 1 MLWHRPGAAYFCALKPYFLCNYFTFATTKCTNKSSSITVMATKTIGESDVGIVSYISALP 60
Query: 61 GFRGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFR 120
GFRGILKQRYSDFIVNEVD DGNVVHLTSLDAPPEIV ES+P V TS+SYASEIELFR
Sbjct: 61 GFRGILKQRYSDFIVNEVDRDGNVVHLTSLDAPPEIVDESRPTTVQNTSDSYASEIELFR 120
Query: 121 SLAGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDG 180
SLAG SDAEILEAFLKQI+SG DDSI+PI+F+PDTDK+HRTA+HNFFKKF FLVTDT+DG
Sbjct: 121 SLAGDSDAEILEAFLKQINSGADDSIAPIIFSPDTDKTHRTAIHNFFKKFKFLVTDTIDG 180
Query: 181 PDSSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENK 240
PDSSSKCVRVRVDSG+QNNRGR S KRKERGDKPFDSRGSDNWPEHVGKFL+FHLYKENK
Sbjct: 181 PDSSSKCVRVRVDSGVQNNRGRSSNKRKERGDKPFDSRGSDNWPEHVGKFLKFHLYKENK 240
Query: 241 DTQEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGN 300
DTQEALGLIGKMLGIQTRSFGF+GTKDKR+I+TQRVTVFKQHASRLAALNDRLIGIKLGN
Sbjct: 241 DTQEALGLIGKMLGIQTRSFGFSGTKDKRSISTQRVTVFKQHASRLAALNDRLIGIKLGN 300
Query: 301 FSYIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVP 360
FSYIQEGLLLGQLLGNRFTITLRGVVADSED IKASAEALGRHGFINYFGLQRFGSGSVP
Sbjct: 301 FSYIQEGLLLGQLLGNRFTITLRGVVADSEDAIKASAEALGRHGFINYFGLQRFGSGSVP 360
Query: 361 THLVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERA 420
THLVGASLLRGEWKGAV+MILDPREGETNVIA+AR+YYKESNDIDGTLKQLPRYLVAERA
Sbjct: 361 THLVGASLLRGEWKGAVSMILDPREGETNVIAKARDYYKESNDIDGTLKQLPRYLVAERA 420
Query: 421 ILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYC 480
ILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYC
Sbjct: 421 ILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYC 480
Query: 481 KENHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGN 540
KENHT T AVQISE DEDCGD N+YDSCHLEE+C LDLPTERNKLVKAVTAED+ SG
Sbjct: 481 KENHTETPAVQISEYE-DEDCGDTNSYDSCHLEEMCKLDLPTERNKLVKAVTAEDILSGI 540
Query: 541 FTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVF 600
FTIDDV+LPLPGSRVIYPTNDIA+VYHDLA KDSI+LTET+HNVKEFSIT+MTGSYR VF
Sbjct: 541 FTIDDVILPLPGSRVIYPTNDIAKVYHDLATKDSISLTETVHNVKEFSITSMTGSYRRVF 600
Query: 601 QRPKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEK-MHDKSKDEDSLDSTEQSG 660
QRP DYEWELISY+DRNLPLA+SDLDNL+ SQ VEIAKEE+ + DKSKDE + E+S
Sbjct: 601 QRPMDYEWELISYLDRNLPLAQSDLDNLVTSQRVEIAKEEENVTDKSKDESNCAEAEESE 660
Query: 661 SCKKSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
CKK+ A+ENENGE ++DPQMALKLSF L SSYATMAIRELLKTSTSVAFHK+LNE
Sbjct: 661 CCKKN---IADENENGEGSEDPQMALKLSFTLSASSYATMAIRELLKTSTSVAFHKSLNE 716
BLAST of CmoCh11G010030 vs. ExPASy TrEMBL
Match:
A0A1S3CHJ1 (pseudouridylate synthase 7 homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501017 PE=3 SV=1)
HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 628/718 (87.47%), Postives = 658/718 (91.64%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
M W+RPG S LKPY LC T K TNRSSSI VM KSIGESDVGILSYISSLPGF
Sbjct: 1 MGWYRPGASSFTVLKPYFLCNSLTAPKLTNRSSSIAVMTTKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSES P VH TS+SY SEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESGPTIVHSTSQSYVSEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AG SDAEILE FLKQI+SG +D ISPIVF+PDT K+HRTAVHNFFKKF FLVTDT+DGPD
Sbjct: 121 AGDSDAEILETFLKQINSGANDDISPIVFSPDTSKTHRTAVHNFFKKFKFLVTDTIDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSG+QNNRGR SKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGVQNNRGRFSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKR+I+TQRVTVFKQHASR+AALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRSISTQRVTVFKQHASRIAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVA+SED IKASAEALG HGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVANSEDVIKASAEALGTHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETN IA+AR YYKES+DIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNEIAKAREYYKESDDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHT TAAVQ SEE DEDCGDAN+YDSCHLEE+C L LPTERNKLVKAVTAEDV SGNFT
Sbjct: 481 NHTETAAVQNSEEYEDEDCGDANSYDSCHLEEMCKLGLPTERNKLVKAVTAEDVLSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRV+YP NDIAEVY+DLA KDSI+LTE++HNVKEFSIT+MTGSYR VFQR
Sbjct: 541 IDDVVLPLPGSRVLYPANDIAEVYNDLATKDSISLTESVHNVKEFSITSMTGSYRRVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLA+SDL LLKSQSVEI+KEEK+ +KSK EDSLDST+QS CK
Sbjct: 601 PKDYEWELISYVDRNLPLAQSDLHKLLKSQSVEISKEEKIANKSKIEDSLDSTDQSVCCK 660
Query: 661 KSNKLQA--NENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALN 717
K +L+A NE E G+ DPQMALKLSF LP SSYATMAIRELLKTSTSVAFHK LN
Sbjct: 661 KDIQLEAGGNETEYGQGTPDPQMALKLSFTLPASSYATMAIRELLKTSTSVAFHKTLN 718
BLAST of CmoCh11G010030 vs. ExPASy TrEMBL
Match:
A0A6J1KIA3 (putative pseudouridine synthase C1A4.09 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496015 PE=3 SV=1)
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 608/617 (98.54%), Postives = 610/617 (98.87%), Query Frame = 0
Query: 101 MKVHGTSESYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTA 160
MK HGTSESYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISPIVF+PDTDKSHRTA
Sbjct: 1 MKGHGTSESYASEIELFRSLAGASDAEILEAFLKQISSGVDDSISPIVFSPDTDKSHRTA 60
Query: 161 VHNFFKKFNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDN 220
VHNFFKKFNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDN
Sbjct: 61 VHNFFKKFNFLVTDTVDGPDSSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDN 120
Query: 221 WPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQH 280
WPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQH
Sbjct: 121 WPEHVGKFLRFHLYKENKDTQEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQH 180
Query: 281 ASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGR 340
ASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVVADSEDTIK SAEALGR
Sbjct: 181 ASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLGNRFTITLRGVVADSEDTIKTSAEALGR 240
Query: 341 HGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESN 400
GFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESN
Sbjct: 241 LGFINYFGLQRFGSGSVPTHLVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESN 300
Query: 401 DIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASM 460
DIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASM
Sbjct: 301 DIDGTLKQLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASM 360
Query: 461 RVQKYGIDKVVVGDLVYCKENHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPT 520
RVQKYGIDKVVVGDLVYCKENHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPT
Sbjct: 361 RVQKYGIDKVVVGDLVYCKENHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPT 420
Query: 521 ERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIH 580
ERNK VKAV+AEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIH
Sbjct: 421 ERNKHVKAVSAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIH 480
Query: 581 NVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKM 640
NVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESDLDNLLKS SVEI KEEKM
Sbjct: 481 NVKEFSITNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESDLDNLLKSLSVEITKEEKM 540
Query: 641 HDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIR 700
HDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIR
Sbjct: 541 HDKSKDEDSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIR 600
Query: 701 ELLKTSTSVAFHKALNE 718
ELLKTSTSVAFHKALNE
Sbjct: 601 ELLKTSTSVAFHKALNE 617
BLAST of CmoCh11G010030 vs. NCBI nr
Match:
XP_022933545.1 (multisubstrate pseudouridine synthase 7 [Cucurbita moschata] >XP_022933546.1 multisubstrate pseudouridine synthase 7 [Cucurbita moschata])
HSP 1 Score: 1413.3 bits (3657), Expect = 0.0e+00
Identity = 717/717 (100.00%), Postives = 717/717 (100.00%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT
Sbjct: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE
Sbjct: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 717
BLAST of CmoCh11G010030 vs. NCBI nr
Match:
XP_023531231.1 (multisubstrate pseudouridine synthase 7 [Cucurbita pepo subsp. pepo] >XP_023531232.1 multisubstrate pseudouridine synthase 7 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1405.2 bits (3636), Expect = 0.0e+00
Identity = 713/717 (99.44%), Postives = 713/717 (99.44%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPY LCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYFLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT
Sbjct: 481 NHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEI KEEKMHDKSKDEDSLDS EQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEITKEEKMHDKSKDEDSLDSIEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE
Sbjct: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 717
BLAST of CmoCh11G010030 vs. NCBI nr
Match:
XP_023002005.1 (multisubstrate pseudouridine synthase 7 isoform X1 [Cucurbita maxima] >XP_023002013.1 multisubstrate pseudouridine synthase 7 isoform X1 [Cucurbita maxima])
HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 707/717 (98.61%), Postives = 709/717 (98.88%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPY LCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYFLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMK HGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKGHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVF+PDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFSPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIK SAEALGR GFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKTSAEALGRLGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPTERNK VKAV+AEDVQSGNFT
Sbjct: 481 NHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPTERNKHVKAVSAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKS SVEI KEEKMHDKSKDEDSLDSTEQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSLSVEITKEEKMHDKSKDEDSLDSTEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 718
KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE
Sbjct: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALNE 717
BLAST of CmoCh11G010030 vs. NCBI nr
Match:
KAG6588316.1 (hypothetical protein SDJN03_16881, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 705/709 (99.44%), Postives = 705/709 (99.44%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPY LCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYFLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAIL 420
Query: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE
Sbjct: 421 QCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKE 480
Query: 481 NHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
NHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT
Sbjct: 481 NHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFT 540
Query: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR
Sbjct: 541 IDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQR 600
Query: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDEDSLDSTEQSGSCK 660
PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEI KEEKMHDKSKDEDSLDSTEQSGSCK
Sbjct: 601 PKDYEWELISYVDRNLPLAESDLDNLLKSQSVEITKEEKMHDKSKDEDSLDSTEQSGSCK 660
Query: 661 KSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSV 710
SNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSV
Sbjct: 661 NSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTSTSV 709
BLAST of CmoCh11G010030 vs. NCBI nr
Match:
KAG7020877.1 (SPBC1A4.09 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1322.4 bits (3421), Expect = 0.0e+00
Identity = 683/730 (93.56%), Postives = 688/730 (94.25%), Query Frame = 0
Query: 1 MRWHRPGISYLCALKPYLLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
MRWHRPGISYLCALKPY LCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF
Sbjct: 1 MRWHRPGISYLCALKPYFLCKPFTITKCTNRSSSITVMAMKSIGESDVGILSYISSLPGF 60
Query: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL
Sbjct: 61 RGILKQRYSDFIVNEVDTDGNVVHLTSLDAPPEIVSESKPMKVHGTSESYASEIELFRSL 120
Query: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD
Sbjct: 121 AGASDAEILEAFLKQISSGVDDSISPIVFAPDTDKSHRTAVHNFFKKFNFLVTDTVDGPD 180
Query: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT
Sbjct: 181 SSSKCVRVRVDSGLQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDT 240
Query: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS
Sbjct: 241 QEALGLIGKMLGIQTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFS 300
Query: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH
Sbjct: 301 YIQEGLLLGQLLGNRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTH 360
Query: 361 LVGASLLRGEWKGAVNMILDPREGETNVI-------------AEARNYYKESNDIDGTLK 420
LVGASLLRGEWKGAVNMILDPREG+ ++ ++ARNYYKESNDIDGTLK
Sbjct: 361 LVGASLLRGEWKGAVNMILDPREGDILLLSALKDKLSLYTSTSKARNYYKESNDIDGTLK 420
Query: 421 QLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGI 480
QLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGI
Sbjct: 421 QLPRYLVAERAILQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGI 480
Query: 481 DKVVVGDLVYCKENHTATAAVQISEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVK 540
DKVVVGDLVYCKENHTATAAVQ SEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVK
Sbjct: 481 DKVVVGDLVYCKENHTATAAVQKSEENVDEDCGDANTYDSCHLEEICTLDLPTERNKLVK 540
Query: 541 AVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSI 600
AVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVK
Sbjct: 541 AVTAEDVQSGNFTIDDVVLPLPGSRVIYPTNDIAEVYHDLAAKDSINLTETIHNVK---- 600
Query: 601 TNMTGSYRSVFQRPKDYEWELISYVDRNLPLAESDLDNLLKSQSVEIAKEEKMHDKSKDE 660
ELISYVDRNLPLAESDLDNLLKSQSVEI KEEKMHDKSKDE
Sbjct: 601 -------------------ELISYVDRNLPLAESDLDNLLKSQSVEITKEEKMHDKSKDE 660
Query: 661 DSLDSTEQSGSCKKSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTST 718
DSLDSTEQSGSCK SNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTST
Sbjct: 661 DSLDSTEQSGSCKNSNKLQANENENGEAAQDPQMALKLSFNLPTSSYATMAIRELLKTST 707
BLAST of CmoCh11G010030 vs. TAIR 10
Match:
AT3G04820.1 (Pseudouridine synthase family protein )
HSP 1 Score: 870.5 bits (2248), Expect = 9.1e-253
Identity = 446/707 (63.08%), Postives = 550/707 (77.79%), Query Frame = 0
Query: 19 LCKPFTITKCTNRSSSITV--MAMKSIGESDVGILSYISSLPGFRGILKQRYSDFIVNEV 78
L KP++ T + ++I MK++ E+DVGI +IS LPGFRGILKQRY+DFIVNEV
Sbjct: 14 LLKPYSSLSWTTKIATIATNFEGMKTVDEADVGISCFISQLPGFRGILKQRYADFIVNEV 73
Query: 79 DTDGNVVHLTSLDAPPE--IVSESKPMKVHGTSESYASEIELFRSLAGASDAEILEAFLK 138
D DGNVVHLTSLD PPE + E K ++Y S +E F+S++ +DAE LE +
Sbjct: 74 DMDGNVVHLTSLDLPPEMAVKEEDKAKSCDNVPQNYTSAVESFKSISSVTDAERLEILIN 133
Query: 139 QISSGVDDSISPIVFAPDTDKSHRTAVHNFFKK-FNFLVTDTVDGPDSSSKCVRVRVDSG 198
Q++SG D + P+V P +DKS RTA+HNF K+ F FLVTDTVDGPDS+SKC+RVRV+SG
Sbjct: 134 QVTSGTDGEVLPVVLDPSSDKSKRTAIHNFVKENFKFLVTDTVDGPDSTSKCIRVRVNSG 193
Query: 199 LQNNRGRVSKKRKERGDKPFDSRGSDNWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGI 258
NNRG SKKRKERGDKPFDSRGS++WPEHVGKFLRFHLYKENKDTQEALGLIGKMLG+
Sbjct: 194 ANNNRGNRSKKRKERGDKPFDSRGSEHWPEHVGKFLRFHLYKENKDTQEALGLIGKMLGV 253
Query: 259 QTRSFGFAGTKDKRAITTQRVTVFKQHASRLAALNDRLIGIKLGNFSYIQEGLLLGQLLG 318
Q +SFGF+GTKDKR+++TQRVTVFKQ AS+LAALN RL GIK+G+F ++EGLLLGQL+G
Sbjct: 254 QPKSFGFSGTKDKRSVSTQRVTVFKQQASKLAALNKRLFGIKVGDFCNVKEGLLLGQLMG 313
Query: 319 NRFTITLRGVVADSEDTIKASAEALGRHGFINYFGLQRFGSGSVPTHLVGASLLRGEWKG 378
NRFTITLRGVVADSE+TIK SAE+LG+ GFINYFGLQRFGSGSVPTH VGA+LL+GEWK
Sbjct: 314 NRFTITLRGVVADSEETIKQSAESLGKDGFINYFGLQRFGSGSVPTHHVGAALLKGEWKD 373
Query: 379 AVNMILDPREGETNVIAEARNYYKESNDIDGTLKQLPRYLVAERAILQCLKKCPGNYLQA 438
AV+MILDPRE E +V+ +AR YYKE+ DIDGTL+QLPRYLVAERAILQCLKKCPGNYLQA
Sbjct: 374 AVDMILDPRETERHVVNDAREYYKETGDIDGTLRQLPRYLVAERAILQCLKKCPGNYLQA 433
Query: 439 LKAIPRTLRMMYVHSYQSYLWNHAASMRVQKYGIDKVVVGDLVYCKENHTATAAVQISEE 498
LK IPRTLRMMYVHSYQSYLWN+AAS+RV+ YG +VV+GDLV K + ++ E
Sbjct: 434 LKGIPRTLRMMYVHSYQSYLWNNAASLRVKNYGTSQVVLGDLVSTKVDAAKRMVDSLTSE 493
Query: 499 NVDEDCGDANTYDSCHLEEICTLDLPTERNKLVKAVTAEDVQSGNFTIDDVVLPLPGSRV 558
+ + D +++ +DLP ER+ LVK V+ ED+++G ++ D+VLPLPGSRV
Sbjct: 494 HNE---SSEEALDCDQVDDTAVVDLPAERSDLVKVVSIEDLEAGTYSTSDIVLPLPGSRV 553
Query: 559 IYPTNDIAEVYHDLAAKDSINLTETIHNVKEFSITNMTGSYRSVFQRPKDYEWELISYVD 618
IYP+NDIAE+YHDLA KD I+LTE+IH VKEFSIT+MTG YR VFQ+P D+EWEL++Y D
Sbjct: 554 IYPSNDIAEIYHDLAKKDGISLTESIHGVKEFSITSMTGGYRRVFQKPIDFEWELLTYTD 613
Query: 619 RNLPLAESDLDNLLKSQSVE-IAKEEKMHDKSKDEDSLDSTEQSGSCKKSNKLQANENEN 678
N PLAE+DLD + ++ VE + E++ D+S D+ + SG ++ +NE E
Sbjct: 614 SNKPLAETDLDRIPMNKPVEKVGSTEEIEDESMKSDT--NPHDSGETNLKDQTDSNEAEK 673
Query: 679 GEA---AQDPQMALKLSFNLPTSSYATMAIRELLKTSTSVAFHKALN 717
++ QMALK++ LP+S YATMAIRELLKTSTSVA+HK LN
Sbjct: 674 DTGNPDSEQTQMALKMALTLPSSCYATMAIRELLKTSTSVAYHKTLN 715
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
O74343 | 1.3e-91 | 34.10 | Multisubstrate pseudouridine synthase 7 OS=Schizosaccharomyces pombe (strain 972... | [more] |
Q08647 | 1.8e-91 | 34.79 | Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC... | [more] |
Q96PZ0 | 7.4e-90 | 32.85 | Pseudouridylate synthase 7 homolog OS=Homo sapiens OX=9606 GN=PUS7 PE=1 SV=2 | [more] |
Q08DI8 | 3.7e-89 | 33.29 | Pseudouridylate synthase 7 homolog OS=Bos taurus OX=9913 GN=PUS7 PE=2 SV=1 | [more] |
Q91VU7 | 6.9e-88 | 32.18 | Pseudouridylate synthase 7 homolog OS=Mus musculus OX=10090 GN=Pus7 PE=2 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F560 | 0.0e+00 | 100.00 | multisubstrate pseudouridine synthase 7 OS=Cucurbita moschata OX=3662 GN=LOC1114... | [more] |
A0A6J1KMR0 | 0.0e+00 | 98.61 | multisubstrate pseudouridine synthase 7 isoform X1 OS=Cucurbita maxima OX=3661 G... | [more] |
A0A6J1DY30 | 0.0e+00 | 86.81 | pseudouridylate synthase 7 homolog OS=Momordica charantia OX=3673 GN=LOC11102559... | [more] |
A0A1S3CHJ1 | 0.0e+00 | 87.47 | pseudouridylate synthase 7 homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035... | [more] |
A0A6J1KIA3 | 0.0e+00 | 98.54 | putative pseudouridine synthase C1A4.09 isoform X2 OS=Cucurbita maxima OX=3661 G... | [more] |
Match Name | E-value | Identity | Description | |
XP_022933545.1 | 0.0e+00 | 100.00 | multisubstrate pseudouridine synthase 7 [Cucurbita moschata] >XP_022933546.1 mul... | [more] |
XP_023531231.1 | 0.0e+00 | 99.44 | multisubstrate pseudouridine synthase 7 [Cucurbita pepo subsp. pepo] >XP_0235312... | [more] |
XP_023002005.1 | 0.0e+00 | 98.61 | multisubstrate pseudouridine synthase 7 isoform X1 [Cucurbita maxima] >XP_023002... | [more] |
KAG6588316.1 | 0.0e+00 | 99.44 | hypothetical protein SDJN03_16881, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
KAG7020877.1 | 0.0e+00 | 93.56 | SPBC1A4.09 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
AT3G04820.1 | 9.1e-253 | 63.08 | Pseudouridine synthase family protein | [more] |