CmoCh11G004630 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G004630
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionomega-6 fatty acid desaturase, chloroplastic-like
LocationCmo_Chr11: 2261455 .. 2264850 (-)
RNA-Seq ExpressionCmoCh11G004630
SyntenyCmoCh11G004630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGATAACAACGCAGCTTTGCACACTAGTGGTGATGGCTCGAAAAGGGCCACCCCTTCAATTCTATCCGTGTATTAGAGAAGGATAAGCCAAGGATTTATACCCAATTTCTCATTTCAAACCGAGGCCTCGCATACTTGAGGAACACCCACTAAGGAAATTCCCCCATTTCCACCGCCATTGCCTCTCATTCAAAGAGCCCAAGCAAGCGAAGGGGGCCTTCTTAGCTGGGATTTCTGCAATTCTGCGTATAATGGCTTGCAGACTTGCGGATTCCTTGTTTCTTTTCACTGTATGTTTCCCGCCATGTCTCATTTTTGGTTCTTGTGTTCTTCGTTTCTTATTGTTTTGGTTTGATTATTAGGGCCCCCATCAGAATTATGCTCGAACCCAGAAAATTGCATCCCATAACGCTCCGGGTATGTATTGGTATCTTCCTTTTGAGTTTTGAGCTATTCAATTAGCTGAATTATCGGCATTCACTTGATAGACTGAATTGCTTAGCAGTTGTCTGATGCAGTAGAAGGGTACATGCTTTGAAGATTGAATTCCATTTGCGTTGTTTTCTTTATCTCAATTTGGGTTGTTTAAGTTTTTCTTTCTGCTGTCCCTTTGAACATAAATAGCTTAGATTTCCATTGTCAATTTTGATGGTTCAAACATGCTTATTCCGATAGACAGGTATATTGGACGTGAAGGGGAAAATAGTATTCCAGAAGAAAATAAAGCGACAAAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCCGTTCCAGTTGCACCACCATCTTCTTCAGCAGACGGTGCTGAATATCGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAGCCACTTCCTGAAAACGTTAGGCTAAAAGATATCATTGAGTCCCTTCCAAAAGAGGTAGTTTATTTGCTGCAACATTGTGGTGTTCGACATCGATCCCGAACTCCCGAGGCATTTGCAACTTTTCGTTGTCAAATAAAATTCTGATGATCTTCAGTTTATGTTTATAGTTACTTACATAGCCTAATTAGTTCATGTTCATGTTTCTTTTCAATTTTTAAAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAGTCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCATTATGATCGCGAAAGCTCCGTGGTATCTACTACCTTTGGCTTGGGCATGGACCGGAACTGCAATAACAGGGGTTAGTCTTATTGCTTTCCTATCTATTCTCTCATACTTGTTGAACTTGCAACTGGGAATACTGAAGTTACTTTCCTTTTTCCTCCTCAAGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGATATTGTGGGTACTTTGGCTTTCATGCCTTTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGTAGGACTAATCTCTCCCCCCTCTCTATCTCTATCAAAAACGCTTATTTATCCGTATCTTCTTGCATATGCATAGTTTGATATCGTTCCATCATATAGTACGAAGACATCTAGTTAAGCAATGCAAAGGCTCTGATCATTAGTATTGGGAGAACAAAATGAATGGGGCTTAAGGTGCCATTATCCATAGCTTGATAGTGCGATGGTTTTTACGCATATCTTATTTTCCCGACACTGTCGAGCATGCAATCGTTCTTCCTTGTATCTTTGGATCAATTAAGCTTGAAAAACTAAATGTATGACTATGAAAATTAGGAATCAATAGCAGGATGACTTAGGTTTCTCCCTTCCATCTTTTATTTTAGCTGAAGTTCGATTTTTATTCTTATCCACGCATGTAGGCTGAAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTCTGCGTAAAGCAATCGTATATGGATATGGTCCGTTTCGTCCGTGGATGTCTATAGCTCATTGGTAAGTCGGATGGTTCCTTTGCAAGAATAACTCGACTTTAATATCGAGATCTCTTGCATGTGATTTATAACCAATTTCTTGCTCTTTCTTTCATTAATATGCAACAATCTGTTCTTATGCATAATAGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTCATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGAATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTAGGCTACCACTTTTGGGTAACATCATTTTTTTGGTTCAAGAATTTAATTGTTCTTTTAGTCACTGTTGTTTACCGACCTACAGTTGTGTCGATAAAAATTACTTTTTATTTCTTGCTTAGATGAGCACTTTCACCATGGTCCATCACACTGCTCCCCATATACCGTTTAAAGCTTCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCATTGTGCTTATCCCCAGTGGTAAACTATAAGTTTTTTCATTGTTTGGGCTAGTTCTAAGCTAATAAGGTGTGTTTAGGTCTAAGCTGCTAATTTTATTTTCTCCCATCGACAGGATCGAGATTCTTTGCCACGACATTAACGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTATAACTTACGAGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGGTAATGGCATTAACTGAATCCATGTCTGCATTGTCTGCCTTGTATATTGCATCTATTTTCCCCTATTGATTCTAACAATCTAGTTCTTTGTTCCATTCCCCACATAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAAACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAAACCCGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTTATGCCTGATCTTGCTTGATTTATCTTCAACCTTTAGGACATACAGACACTTTGCTTGTTTCTTTCTCCGCAAACTTTGAGGAATGAATGGAAGGAATCTTGGTGGCCTGCCTTGTACTTAGTTAACAGAAAGTAATGATTTGGTGAACCATAAAAGAGAGACCATGTGATTTCTGAAACAGAGAAATGTTATGTTTAACAACTTTAGAGCCGTAGGGGTTTGGTTTTGATTCTTTTGGTATTAGCAGTTGTTTCTCTCTGCTTGTCCTCTCCATAGATTGGTATCACTTTCACCACCAGATCTTTGGTTGGTTCATATCTCAGTGAGTGTTCGTTTAATAAAACTGTCCTTTTCTTAAGAACAGAGGTTGAGATGAAAGAATATTGAGATTATTAATTCATGTA

mRNA sequence

ATGAGTGATAACAACGCAGCTTTGCACACTAGTGGTGATGGCTCGAAAAGGGCCACCCCTTCAATTCTATCCGTGAAATTCCCCCATTTCCACCGCCATTGCCTCTCATTCAAAGAGCCCAAGCAAGCGAAGGGGGCCTTCTTAGCTGGGATTTCTGCAATTCTGCGTATAATGGCTTGCAGACTTGCGGATTCCTTGTTTCTTTTCACTGGCCCCCATCAGAATTATGCTCGAACCCAGAAAATTGCATCCCATAACGCTCCGGGTATATTGGACGTGAAGGGGAAAATAGTATTCCAGAAGAAAATAAAGCGACAAAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCCGTTCCAGTTGCACCACCATCTTCTTCAGCAGACGGTGCTGAATATCGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAGCCACTTCCTGAAAACGTTAGGCTAAAAGATATCATTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAGTCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCATTATGATCGCGAAAGCTCCGTGGTATCTACTACCTTTGGCTTGGGCATGGACCGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGATATTGTGGGTACTTTGGCTTTCATGCCTTTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGAAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTCTGCGTAAAGCAATCGTATATGGATATGGTCCGTTTCGTCCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTCATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGAATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTAGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACTGCTCCCCATATACCGTTTAAAGCTTCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCATTGTGCTTATCCCCAGTGGATCGAGATTCTTTGCCACGACATTAACGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTATAACTTACGAGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAAACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAAACCCGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTTATGCCTGATCTTGCTTGATTTATCTTCAACCTTTAGGACATACAGACACTTTGCTTGTTTCTTTCTCCGCAAACTTTGAGGAATGAATGGAAGGAATCTTGGTGGCCTGCCTTGTACTTAGTTAACAGAAAGTAATGATTTGGTGAACCATAAAAGAGAGACCATGTGATTTCTGAAACAGAGAAATGTTATGTTTAACAACTTTAGAGCCGTAGGGGTTTGGTTTTGATTCTTTTGGTATTAGCAGTTGTTTCTCTCTGCTTGTCCTCTCCATAGATTGGTATCACTTTCACCACCAGATCTTTGGTTGGTTCATATCTCAGTGAGTGTTCGTTTAATAAAACTGTCCTTTTCTTAAGAACAGAGGTTGAGATGAAAGAATATTGAGATTATTAATTCATGTA

Coding sequence (CDS)

ATGAGTGATAACAACGCAGCTTTGCACACTAGTGGTGATGGCTCGAAAAGGGCCACCCCTTCAATTCTATCCGTGAAATTCCCCCATTTCCACCGCCATTGCCTCTCATTCAAAGAGCCCAAGCAAGCGAAGGGGGCCTTCTTAGCTGGGATTTCTGCAATTCTGCGTATAATGGCTTGCAGACTTGCGGATTCCTTGTTTCTTTTCACTGGCCCCCATCAGAATTATGCTCGAACCCAGAAAATTGCATCCCATAACGCTCCGGGTATATTGGACGTGAAGGGGAAAATAGTATTCCAGAAGAAAATAAAGCGACAAAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCCGTTCCAGTTGCACCACCATCTTCTTCAGCAGACGGTGCTGAATATCGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAGCCACTTCCTGAAAACGTTAGGCTAAAAGATATCATTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAGTCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCATTATGATCGCGAAAGCTCCGTGGTATCTACTACCTTTGGCTTGGGCATGGACCGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGATATTGTGGGTACTTTGGCTTTCATGCCTTTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGAAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTCTGCGTAAAGCAATCGTATATGGATATGGTCCGTTTCGTCCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAATGAAGTGAAAAGAGTGAAGATAAGCCTAGCTTGCGTTTTTGCATTCATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGAATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTAGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACTGCTCCCCATATACCGTTTAAAGCTTCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCATTGTGCTTATCCCCAGTGGATCGAGATTCTTTGCCACGACATTAACGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTATAACTTACGAGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAAACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTACGTCGCTTTTGACCGACTCAAACCCGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTTATGCCTGATCTTGCTTGA

Protein sequence

MSDNNAALHTSGDGSKRATPSILSVKFPHFHRHCLSFKEPKQAKGAFLAGISAILRIMACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAILRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLKPEDSYPITFLKKVMPDLA
Homology
BLAST of CmoCh11G004630 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 5.9e-211
Identity = 341/445 (76.63%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHN-APGILDVKGKIVFQKKIKRQNYLLPV-KRA 117
           MA R+ADSLF FTGP Q   R  K+AS   +PG+  V+  I    K  R+ +L+P  KR 
Sbjct: 1   MASRIADSLFAFTGPQQCLPRAPKLASARLSPGVYAVR-PIDLLLKGTRRTFLVPAKKRI 60

Query: 118 AILRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEIN 177
             ++AV VPVAPP  SAD AE R+QL+ESYGF+QIG+ LP+NV LKDI+++LPKEVFEI+
Sbjct: 61  GCIKAVFVPVAPP--SADNAEDREQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEID 120

Query: 178 DVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNK 237
           DVKAWKSVL+SV SY LGL MIAKAPWYLLPLAWAWTGTA+TGFFVIGHDCAHKSFS+NK
Sbjct: 121 DVKAWKSVLISVTSYALGLFMIAKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSKNK 180

Query: 238 LVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIV 297
           LVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEFDS+P+LRKAI+
Sbjct: 181 LVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFDSSPVLRKAII 240

Query: 298 YGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGW 357
           +GYGP RPW+SIAHW+ WHF+L+KFRP+EV RVKISLACVFAFM +GWPLIIYK G++GW
Sbjct: 241 FGYGPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGWPLIIYKVGVLGW 300

Query: 358 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCH 417
           VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCH
Sbjct: 301 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCH 360

Query: 418 DINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENY 477
           DINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY KEENY
Sbjct: 361 DINVHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENY 420

Query: 478 VAFDRLKPEDSYPITFLKKVMPDLA 501
           + FDRL PE+S PITFLKK MPD A
Sbjct: 421 IPFDRLAPEESQPITFLKKAMPDYA 442

BLAST of CmoCh11G004630 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 721.1 bits (1860), Expect = 8.7e-207
Identity = 336/447 (75.17%), Postives = 385/447 (86.13%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNA---PGILDVK--GKIVFQKKIKRQNYLLPV 117
           MA R+ADSLF FTGP Q   R  K+A+ +A   PG+  VK    ++  +  + +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 118 K-RAAILRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEV 177
           K R   ++AVA PVAPP  SAD AE R+QL+ESYGF QIGE LPENV LKDI+++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 178 FEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 237
           FEI+D+KA KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 238 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLR 297
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 298 KAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 357
           KAI++GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 358 IIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIE 417
           I+GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 418 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSK 477
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 478 EENYVAFDRLKPEDSYPITFLKKVMPD 499
           EENY+ FDRL PE+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of CmoCh11G004630 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.2e-203
Identity = 323/426 (75.82%), Postives = 364/426 (85.45%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MAC LADSL LF G +Q     + IA+  +PGI  +    + QK+ +RQ   +   +  +
Sbjct: 1   MACTLADSLLLFKGSYQKPVLRRDIAARYSPGIFSLNSNGLIQKRFRRQRNFVTRNKVTV 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           + AVA+PV P  +  + AEYRKQL+E YGF Q+GEPL ++V LKD+I  LPKEVFEI+DV
Sbjct: 61  IHAVAIPVQP--APVESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVL+SV SY LGL MI+KAPWYLLPLAW WTGTAITGFFVIGHDCAH+SFS NKLV
Sbjct: 121 KAWKSVLISVTSYALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHD+HHAKTNML+EDTAWHPVWK+EF+S PLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFR WMSIAHWL+WHFDLKKFRP+EV RVKISLACVFAF+ IGWPLIIYKTGI+GW+K
Sbjct: 241 YGPFRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YP+WIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSLQENWG++LNEASWNWRLMKTIMT+C VY KE++   
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCC 420

Query: 478 FDRLKP 484
             R  P
Sbjct: 421 LRRTCP 424

BLAST of CmoCh11G004630 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.2e-185
Identity = 292/390 (74.87%), Postives = 343/390 (87.95%), Query Frame = 0

Query: 113 KRAAILRAVAVPVAPPSS--SADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKE 172
           +++ +++AVAVPVA PS+    D  E+ KQL+E YGF+QIGEPLP++V ++DII SLPK+
Sbjct: 58  RKSTLVQAVAVPVAQPSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPKQ 117

Query: 173 VFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 232
           VFEIND KAW +VL+SV SY LG+ MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS
Sbjct: 118 VFEINDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 177

Query: 233 FSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLL 292
           FS+NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHH KTNML+EDTAW P+ KE+ +S+P L
Sbjct: 178 FSKNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIMKEDIESSPGL 237

Query: 293 RKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKT 352
           RKA++Y YGP R WMSIAHWL  HF+LK FR +EVKR  ISLA VFAFM IGWPLIIYKT
Sbjct: 238 RKALIYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMVIGWPLIIYKT 297

Query: 353 GIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWI 412
           GI+GW+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFK+S+EWNAAQAQL+GTVHC YP+WI
Sbjct: 298 GIVGWIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSGTVHCDYPRWI 357

Query: 413 EILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYS 472
           EILCHDI+VHIPHHISP+IPSYNLRAA++SL ENWG++LN+   NWRLM+TIMT CH+Y 
Sbjct: 358 EILCHDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMRTIMTTCHIYD 417

Query: 473 KEENYVAFDRLKPEDSYPITFLKKVMPDLA 501
           K+ NYV+F++  PE+S PI+  K+VMPD A
Sbjct: 418 KDGNYVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of CmoCh11G004630 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.7e-107
Identity = 175/327 (53.52%), Postives = 227/327 (69.42%), Query Frame = 0

Query: 157 NVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAI 216
           +++L+DII++LPKE FE    KAW SVL+++ +  +G + I   PWY LP+ W WTGTA+
Sbjct: 23  DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWTGTAL 82

Query: 217 TGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAW 276
           TG FV+GHDC H+SF++ + V D+VG +AF PLIYP+  WR  HD HH  TN ++ D AW
Sbjct: 83  TGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHTNKIEVDNAW 142

Query: 277 HPVWKEEFDSAPLLRKAIVYGY-GPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACV 336
            P   E F ++P + +       GPF    SI HW L HF L  F   +  +VK+S+A V
Sbjct: 143 DPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRNKVKLSIAVV 202

Query: 337 FAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQ 396
           F F  I +P +I  TG+ G+VKFWLMPWL YHFWMSTFT+VHHT P I F+ + +W+AA+
Sbjct: 203 FLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFRPAADWSAAE 262

Query: 397 AQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWN 456
           AQLNGTVHC YP+W+E+LCHDINVHIPHH+S  IPSYNLR AH SL+ENWG  L E ++N
Sbjct: 263 AQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWGPFLYERTFN 322

Query: 457 WRLMKTIMTICHVYSKEENYVAFDRLK 483
           W+LM+ I   CH+Y  E  Y  F  LK
Sbjct: 323 WQLMQQISGQCHLYDPEHGYRTFGSLK 349

BLAST of CmoCh11G004630 vs. ExPASy TrEMBL
Match: A0A6J1EQE5 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436860 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.6e-270
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRLKPEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of CmoCh11G004630 vs. ExPASy TrEMBL
Match: A0A6J1HLM6 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465334 PE=4 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.2e-268
Identity = 438/443 (98.87%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGL++IAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRLKPEDSYPI FL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of CmoCh11G004630 vs. ExPASy TrEMBL
Match: A0A0A0LTB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G255170 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.0e-258
Identity = 417/443 (94.13%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRL DS+ LF GPH N+ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRA+AVP APPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKD++ESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CmoCh11G004630 vs. ExPASy TrEMBL
Match: A0A1S3B9S5 (omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487358 PE=4 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.6e-257
Identity = 416/443 (93.91%), Postives = 432/443 (97.52%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRL DS+ LF GPHQN ARTQKIASH +PG L VKG+I+FQKKIKRQNYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPV KEEFDSAP+LRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CmoCh11G004630 vs. ExPASy TrEMBL
Match: A0A6J1C240 (omega-6 fatty acid desaturase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007246 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.0e-255
Identity = 413/443 (93.23%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADS+FLFTGPHQN ARTQK ASH +PGIL VKG+I+FQKKIKR+NYLLPVKRAAI
Sbjct: 1   MACRLADSMFLFTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPP +SAD +EYRKQLSESYGFE+IGEPLPENVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPP-TSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YG
Sbjct: 181 EDIVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHF+LK FRPNEVKRVKISLACVFAFMFIGWPLIIYK GI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSLQENWGK+LNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRL PEDSYPITFL+KVMPD+A
Sbjct: 421 FDRLNPEDSYPITFLRKVMPDIA 442

BLAST of CmoCh11G004630 vs. NCBI nr
Match: KAG6587828.1 (hypothetical protein SDJN03_16393, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 960.7 bits (2482), Expect = 5.0e-276
Identity = 463/500 (92.60%), Postives = 464/500 (92.80%), Query Frame = 0

Query: 1   MSDNNAALHTSGDGSKRATPSILSVKFPHFHRHCLSFKEPKQAKGAFLAGISAILRIMAC 60
           MSDNNAALHTSGDGSKRATPSILSV                                   
Sbjct: 1   MSDNNAALHTSGDGSKRATPSILSV----------------------------------- 60

Query: 61  RLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAILRA 120
            LADSLFLFTGPHQNYARTQKIASHNAPGI+DVKGKIVFQKKIKRQNYLLPVKRAAILRA
Sbjct: 61  -LADSLFLFTGPHQNYARTQKIASHNAPGIMDVKGKIVFQKKIKRQNYLLPVKRAAILRA 120

Query: 121 VAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDVKAW 180
           VAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDVKAW
Sbjct: 121 VAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDVKAW 180

Query: 181 KSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI 240
           KSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI
Sbjct: 181 KSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI 240

Query: 241 VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGP 300
           VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGP
Sbjct: 241 VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGP 300

Query: 301 FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWL 360
           FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWL
Sbjct: 301 FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWL 360

Query: 361 MPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 420
           MPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH
Sbjct: 361 MPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 420

Query: 421 IPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDR 480
           IPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDR
Sbjct: 421 IPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDR 464

Query: 481 LKPEDSYPITFLKKVMPDLA 501
           LKPEDSYPITFLKKVMPDLA
Sbjct: 481 LKPEDSYPITFLKKVMPDLA 464

BLAST of CmoCh11G004630 vs. NCBI nr
Match: XP_022930407.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 940.6 bits (2430), Expect = 5.4e-270
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRLKPEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of CmoCh11G004630 vs. NCBI nr
Match: XP_023529482.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 938.7 bits (2425), Expect = 2.0e-269
Identity = 440/443 (99.32%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVH+PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHVPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRLKPEDSYPITFL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLRKVMPDLA 443

BLAST of CmoCh11G004630 vs. NCBI nr
Match: XP_022965421.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 933.7 bits (2412), Expect = 6.5e-268
Identity = 438/443 (98.87%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 237
           KAWKSVLVSVASYTLGL++IAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 238 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 297
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 298 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 357
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 358 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 417
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 418 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 477
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 478 FDRLKPEDSYPITFLKKVMPDLA 501
           FDRLKPEDSYPI FL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of CmoCh11G004630 vs. NCBI nr
Match: KAG7035676.1 (hypothetical protein SDJN02_02474 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 924.9 bits (2389), Expect = 3.0e-265
Identity = 443/473 (93.66%), Postives = 443/473 (93.66%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 117
           MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 118 LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 177
           LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 178 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITG------------------- 237
           KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITG                   
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGVSLIAFLSILSYLLDLQLG 180

Query: 238 -----------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT 297
                      FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT
Sbjct: 181 ILKLLSFFLLKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT 240

Query: 298 NMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR 357
           NMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR
Sbjct: 241 NMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR 300

Query: 358 VKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKA 417
           VKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKA
Sbjct: 301 VKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKA 360

Query: 418 SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGK 477
           SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGK
Sbjct: 361 SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGK 420

Query: 478 HLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLKPEDSYPITFLKKVMPDLA 501
           HLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLKPEDSYPITFLKKVMPDLA
Sbjct: 421 HLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLKPEDSYPITFLKKVMPDLA 473

BLAST of CmoCh11G004630 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 721.1 bits (1860), Expect = 6.2e-208
Identity = 336/447 (75.17%), Postives = 385/447 (86.13%), Query Frame = 0

Query: 58  MACRLADSLFLFTGPHQNYARTQKIASHNA---PGILDVK--GKIVFQKKIKRQNYLLPV 117
           MA R+ADSLF FTGP Q   R  K+A+ +A   PG+  VK    ++  +  + +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 118 K-RAAILRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEV 177
           K R   ++AVA PVAPP  SAD AE R+QL+ESYGF QIGE LPENV LKDI+++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 178 FEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 237
           FEI+D+KA KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 238 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLR 297
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 298 KAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 357
           KAI++GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 358 IIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIE 417
           I+GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 418 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSK 477
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 478 EENYVAFDRLKPEDSYPITFLKKVMPD 499
           EENY+ FDRL PE+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of CmoCh11G004630 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 130.2 bits (326), Expect = 4.7e-30
Identity = 98/344 (28.49%), Postives = 154/344 (44.77%), Query Frame = 0

Query: 151 GEPLPENVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAP----WYLLP 210
           G P P N  L DI  ++PK  +  N    WKS+   V    +   + A A     W + P
Sbjct: 91  GAPPPFN--LADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWP 150

Query: 211 LAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAK 270
           L W   GT     FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H  HH  
Sbjct: 151 LYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQN 210

Query: 271 TNMLKEDTAWHPVWKEEFD-----------SAPLLRKAIVYGYGPFRPWMSIAHWLLWHF 330
              ++ D +WHP+ ++ ++           + PL+  A  +      P    +H   +H 
Sbjct: 211 HGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH---YHP 270

Query: 331 DLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTM 390
           D   F P E K V  S AC  A   +    + +  G I  +K + +P+     W+   T 
Sbjct: 271 DSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTY 330

Query: 391 VHHTA--PHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYN 450
           +HH      +P+   +EW+  +  L  T+   Y   I  + HDI  H+ HH+ P+IP Y+
Sbjct: 331 LHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQIPHYH 390

Query: 451 LRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 478
           L  A ++ +   GK+  E   +  L   ++ I     KE++YV+
Sbjct: 391 LVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of CmoCh11G004630 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 126.7 bits (317), Expect = 5.2e-29
Identity = 84/334 (25.15%), Postives = 159/334 (47.60%), Query Frame = 0

Query: 159 RLKDIIESLPKEVFEINDVKAWKSV---LVSVASYTLGLIMIAKAPWYLLPLAWAWTGTA 218
           ++ DI  ++PK  +  + +++   V   +++VA+  +  + +    W+L PL WA  GT 
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS--WFLWPLYWAAQGTL 94

Query: 219 ITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTA 278
               FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH     ++ D +
Sbjct: 95  FWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDES 154

Query: 279 WHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLW--------HFD--LKKFRPNEV 338
           W P+ +  +   P   + + Y      P ++   +L +        HF+     F P+E 
Sbjct: 155 WVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSSLFAPSER 214

Query: 339 KRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA--PHI 398
           K +  S  C ++ MF+    + +  G +  +K + +P++ +  W+   T +HH      +
Sbjct: 215 KLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKL 274

Query: 399 PFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQE 458
           P+   +EW+  +  L  T+   Y  +  I  HDI  H+ HH+ P+IP Y+L  A K+ + 
Sbjct: 275 PWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVDATKAAKH 334

Query: 459 NWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 478
             G++  E   +  +   ++       K+++YV+
Sbjct: 335 VLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of CmoCh11G004630 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-28
Identity = 93/313 (29.71%), Postives = 140/313 (44.73%), Query Frame = 0

Query: 151 GEPLPENVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAK--APWYLLPLA 210
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 211 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 270
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 271 MLKEDTAWHP----VWKEEFDSAPLLRKAIVYGY--GPFRPWMSI--AHWLLWHFDLKKF 330
            ++ D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 331 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA 390
            P E K V  S AC  A   +    + +  G I  +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 391 --PHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAH 450
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L  A 
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLVEAT 383

Query: 451 KSLQENWGKHLNE 452
           ++ +   GK+  E
Sbjct: 384 EAAKPVLGKYYRE 390

BLAST of CmoCh11G004630 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 120.9 bits (302), Expect = 2.8e-27
Identity = 89/296 (30.07%), Postives = 132/296 (44.59%), Query Frame = 0

Query: 151 GEPLPENVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAK--APWYLLPLA 210
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 211 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 270
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 271 MLKEDTAWHP----VWKEEFDSAPLLRKAIVYGY--GPFRPWMSI--AHWLLWHFDLKKF 330
            ++ D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 331 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA 390
            P E K V  S AC  A   +    + +  G I  +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 391 --PHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNL 435
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHL 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P486275.9e-21176.63Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1[more]
P463128.7e-20775.17Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P486281.2e-20375.82Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1[more]
P486293.2e-18574.87Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD... [more]
P203882.7e-10753.52Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
A0A6J1EQE52.6e-270100.00omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1HLM63.2e-26898.87omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0LTB11.0e-25894.13FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G2551... [more]
A0A1S3B9S55.6e-25793.91omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1C2404.0e-25593.23omega-6 fatty acid desaturase, chloroplastic OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
KAG6587828.15.0e-27692.60hypothetical protein SDJN03_16393, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022930407.15.4e-270100.00omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata][more]
XP_023529482.12.0e-26999.32omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022965421.16.5e-26898.87omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita maxima][more]
KAG7035676.13.0e-26593.66hypothetical protein SDJN02_02474 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT4G30950.16.2e-20875.17fatty acid desaturase 6 [more]
AT3G11170.14.7e-3028.49fatty acid desaturase 7 [more]
AT2G29980.15.2e-2925.15fatty acid desaturase 3 [more]
AT5G05580.11.5e-2829.71fatty acid desaturase 8 [more]
AT5G05580.22.8e-2730.07fatty acid desaturase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 202..448
e-value: 8.1E-30
score: 104.5
NoneNo IPR availablePANTHERPTHR32100:SF67BNAC03G67820D PROTEINcoord: 58..500
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 58..500
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 178..434
e-value: 8.17881E-80
score: 246.368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G004630.1CmoCh11G004630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane