CmoCh11G004360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G004360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionhistone-lysine N-methyltransferase ASHR3
LocationCmo_Chr11: 2141366 .. 2144676 (-)
RNA-Seq ExpressionCmoCh11G004360
SyntenyCmoCh11G004360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATAACACCTTCCGATTTGAGAGTGCTAGATGTTCTTCAGATGAATGCCCGTGCCCAAGGTAATAGCTTCTTGTGGTCGCTTTCAATTGTTAAATGAAATGATCACCTTTTTCTTTTGAAGTAAAACACTTCCTCTGTTGTAGCATGCCTAGAGGGATGAATACGTTTGTTCTTGGCAAAATCCATGTAAAATAATGGTTGCGTGAGAGATTTATTGTCTTGTGTTGTTTTATTTGGCAATGAAGATATTAGCATTGTGACACTGTATGTCATTCAGGCGTATAATGTTTTTTTCCCTTGAAAAATGAAGATACCTTGTTTTAATGCTTATTTATCTATAGATAGTTTTCTTGTCTTTGACCACTTTCTCTGTTTTTTAATTCTTTCTTTCTTTCTTTCTAGTTAAAGTTTTACTTCTACATCTGTATCTTTATTATTTAGGCGGGCTGACAGGACTATTGTATTTGTTCAATGGGCAGCATGCAGTGTCAGTAAGTGACATGGAGGTTCGATTCACAAACAATTTAATTTGTTGGATTCTCTTACCCTCTCCTTGATGAATATGTTTTTAAGAAAATAAATAAAAGATTTTTATCAAAAAGAAAAAGAAAAAAGAAAAGCAGAATGGCACGCACGCAAGTTGGAGAACAGACGGTTAGTTTCTTAACACTCCCTTTAATGAATTTGAAGCTTGCCATTTTCATTGGTCAACGGTTAGATGGTGTTGGACTCTCACACTTGCTGTGGTAGAGTCTATTGGTTTTTCAATATTCTAAATTAAATTTTCCGTGTTGGAGATTTTGCATGGCCATAGAAAGACAGTTATCATCTTTTTCTTTCTGGGACAAAAGATGATAATCATGCTGAACCATCCCGTATATGTTTATATTCATGATACATATGAAGTATTTGAGAAATTATAAACTTTATATGGAGAAGAATTTTAGATTCATAGCAATGCCGTTGTAAGTTCCATTCCGCACTCTGGATTGCGCATTAATTGAAGTCTGGCGTGAATCACGACTCACGACAGACTCATCTTCCAAGTGTTTATCAGTTGGGATATCGTTTTAACTAAAGATCCCTATGTTCCTCTGTATTTCCTCCTGCAATTTATTATTTCATGCACTTGGGTTATGATTTGCATTCGTCTAAAAAAGGATATGACTTGCAATTGTTTGTTATGAATTTGTAGATTCTTTTAAAGAACAATTTGTGCGTATCAGACAAGTACTGATTCCATGCTTGCCTCATCGTACTAGTCCAAGATTTTCTGACCAATAATATTTGGTTTTGTGAATAGGCAAGTTGGGGGAGAAACACGTGTTGGTGTCTTTGCTGCTCGATCAATCGAGGTTGGAGAGCCATTAACATATGAATACAGGTACGTAAAAAGGATGGTCTAAGCTTTAATCATACTTTCAAATAACAAGGAAATCATCTTGATGCTGTGTGGAAAATCTAACATTATGGGGTACATTTTAAAACTACAACTGTTAATTGATCAGCTTGCTTACCATTGAGTTTCTTGAGAAGCTTTTTGGGTGTGGATGAATGTGCATGTTGCATTTGATATTGAACAAGGATTTTTCATCATGAAGATTTGTGCAATTTGGACCTGAAGTGAAATGCCATTGTGGTGCTCCAAACTGTCAACGCTATCTTGGAACCAAAAAAATAAGTGCCTTGGACCTTTTCTGGGGTACGAAACGCAGGAGAACGTCAGCAGCTCACATAACTGGTCGGTGTAGTTGGGTCAATATCGCTGTACATTATAACTTGATATAAGTGGGCAAACACGCAAGAAAAATAGGAGCAAAATAAGTACGAATGCCATTACATTCTTCGACTTTCAACAACTATTGCAAAGTGCTCCCGAATTTTCTTGTTGGCGAACGTTGAAATGGAGTAATACGTGTGATTGATTTTACCCGATTGGTCTAAAGAGACCTAGGAATTCTTTTGTAGTTTATTAAATGCTTGACGTTCTTACTCCTTGGAGATGATTAGATGAATGAAGTTCAGTCCAAGAATCCATTTGATAACCATTATGTTTAGTTGAGAAGATTACTCTTATGCTAAAGAAATACTTGGTTCTATTAAGATAATACGTCATAATCTGATTCTGTACGTATCCTATGATAGGAATCATCCCATGTGATTACAAAGCGGATTCTATTAACAATCTTCCCTTGAGAAAATTCTTGCAGATTTTCTCCCACCGCCGTGTATCTGGTTCTGCCGCTTGCACTCCCAGCTTTGCATCTTCAGCAAATTCCCTCTTCAAAAGACCATAAACTTCAATCACCAGTTACAAGGTAAAAAAAAAAAGGTGATGAAATTGTAATCTGTAAGTACTTGCTGATAAAAGTATTAGGAAAACATTGACCTGCATGGTATCGTTATTTAGGAGACGATCCACGAGATTGTAGAGAATGTCTTTGGAATGCAAAGGGTGGCCTTGATGCCTAAAATATGTCCTCCGATGGCCAAGATTTCAACGGCTGTCTGGTCTGAGAGGCCCATGACTTGAGCTCCAAAAGGTATTTCCCAAACTTCATCTTGATGGCATTCAATTATGGACAGACTGCTTGGAATTTCACCCATCTCTTCATTAAATTTCCACTCTGAACTCTCCATGATTTCCACTTTTCTCACCTCAATATCCCACCCTGTCTCAGCCTTCCCAACTATCCCACCCAATGTCCTACACAACACCTGTCATGAGCACACACAAGTCCATTTCTCAATACAATGTTTTAGGTATATTATCAACCCATAATAGTCTCTAATTTTACTATTAGTTTTCCATGAAATTTACTTATTGTTTCAAAAATATTCATATTCTTTCTTTTTCTATTTGTTTCCAAATTATCCTTAAATAGAAAAAAAAATGTTGGAATGCAAAATCAATGAGTTTGAGTGTTACTTTTGGACTTGATCTGGACAAATGCCAAGATATATAATACACTACTTGATGTCCAAAACAATGCCAAGAAGCTTCTTCTCCATAGCATCAAGGGTCTGCAAGAGGAAATGGAGACCCAAAATCCAAGAGCCATTGGCATAAGCATCATGCGGGCTTCCACTGATCTCAAAACCATCATAGTTTTGAAGCACTCTCCTTCCACCACCATAAACAAATCTCATCTCTCTCCTTCTCCAAAAGCTGCAACGAACAGTTCAAAATATCCTCCGTACACTTTCTTTACATATTCCGAGTCCTTTGCTGCCTGCAACAACGCATATCTTTTTCCACCACCTTCTACCTCCACTTCCATCTCCACCACTACTATTAACAAATAA

mRNA sequence

ATGAATATAACACCTTCCGATTTGAGAGTGCTAGATGTTCTTCAGATGAATGCCCGTGCCCAAGGCGGGCTGACAGGACTATTGTATTTGTTCAATGGGCAGCATGCAGTGTCAGTAAGTGACATGGAGATTTTGCATGGCCATAGAAAGACAGTTATCATCTTTTTCTTTCTGGGACAAAAGATGATAATCATGCTGAACCATCCCATTCTTTTAAAGAACAATTTGTGCGTATCAGACAAGCAAGTTGGGGGAGAAACACGTGTTGGTGTCTTTGCTGCTCGATCAATCGAGGTTGGAGAGCCATTAACATATGAATACAGATTTGTGCAATTTGGACCTGAAGTGAAATGCCATTGTGGTGCTCCAAACTGTCAACGCTATCTTGGAACCAAAAAAATAAGTGCCTTGGACCTTTTCTGGGGTACGAAACGCAGGAGAACGTCAGCAGCTCACATAACTGGTCGATTTTCTCCCACCGCCGTGTATCTGGTTCTGCCGCTTGCACTCCCAGCTTTGCATCTTCAGCAAATTCCCTCTTCAAAAGACCATAAACTTCAATCACCAGTTACAAGCATCAAGGGTCTGCAAGAGGAAATGGAGACCCAAAATCCAAGAGCCATTGGCATAAGCATCATGCGGGCTTCCACTGATCTCAAAACCATCATAGTTTTGAAGCACTCTCCTTCCACCACCATAAACAAATCTCATCTCTCTCCTTCTCCAAAAGCTGCAACGAACAGTTCAAAATATCCTCCGTACACTTTCTTTACATATTCCGAGTCCTTTGCTGCCTGCAACAACGCATATCTTTTTCCACCACCTTCTACCTCCACTTCCATCTCCACCACTACTATTAACAAATAA

Coding sequence (CDS)

ATGAATATAACACCTTCCGATTTGAGAGTGCTAGATGTTCTTCAGATGAATGCCCGTGCCCAAGGCGGGCTGACAGGACTATTGTATTTGTTCAATGGGCAGCATGCAGTGTCAGTAAGTGACATGGAGATTTTGCATGGCCATAGAAAGACAGTTATCATCTTTTTCTTTCTGGGACAAAAGATGATAATCATGCTGAACCATCCCATTCTTTTAAAGAACAATTTGTGCGTATCAGACAAGCAAGTTGGGGGAGAAACACGTGTTGGTGTCTTTGCTGCTCGATCAATCGAGGTTGGAGAGCCATTAACATATGAATACAGATTTGTGCAATTTGGACCTGAAGTGAAATGCCATTGTGGTGCTCCAAACTGTCAACGCTATCTTGGAACCAAAAAAATAAGTGCCTTGGACCTTTTCTGGGGTACGAAACGCAGGAGAACGTCAGCAGCTCACATAACTGGTCGATTTTCTCCCACCGCCGTGTATCTGGTTCTGCCGCTTGCACTCCCAGCTTTGCATCTTCAGCAAATTCCCTCTTCAAAAGACCATAAACTTCAATCACCAGTTACAAGCATCAAGGGTCTGCAAGAGGAAATGGAGACCCAAAATCCAAGAGCCATTGGCATAAGCATCATGCGGGCTTCCACTGATCTCAAAACCATCATAGTTTTGAAGCACTCTCCTTCCACCACCATAAACAAATCTCATCTCTCTCCTTCTCCAAAAGCTGCAACGAACAGTTCAAAATATCCTCCGTACACTTTCTTTACATATTCCGAGTCCTTTGCTGCCTGCAACAACGCATATCTTTTTCCACCACCTTCTACCTCCACTTCCATCTCCACCACTACTATTAACAAATAA

Protein sequence

MNITPSDLRVLDVLQMNARAQGGLTGLLYLFNGQHAVSVSDMEILHGHRKTVIIFFFLGQKMIIMLNHPILLKNNLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKKISALDLFWGTKRRRTSAAHITGRFSPTAVYLVLPLALPALHLQQIPSSKDHKLQSPVTSIKGLQEEMETQNPRAIGISIMRASTDLKTIIVLKHSPSTTINKSHLSPSPKAATNSSKYPPYTFFTYSESFAACNNAYLFPPPSTSTSISTTTINK
Homology
BLAST of CmoCh11G004360 vs. ExPASy Swiss-Prot
Match: Q949T8 (Histone-lysine N-methyltransferase ASHR3 OS=Arabidopsis thaliana OX=3702 GN=ASHR3 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 57/85 (67.06%), Postives = 64/85 (75.29%), Query Frame = 0

Query: 66  LNHPILLKNNLCVSDK-QVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPN 125
           LNH     N  CV +K QV GETRVGVFAAR IE GEPLTY+YRFVQFGPEVKC+CG+ N
Sbjct: 403 LNHSC---NPNCVLEKWQVEGETRVGVFAARQIEAGEPLTYDYRFVQFGPEVKCNCGSEN 462

Query: 126 CQRYLGTKK--ISALDLFWGTKRRR 148
           CQ YLGTK+   + L + WG KRRR
Sbjct: 463 CQGYLGTKRKEPNCLVVSWGAKRRR 484

BLAST of CmoCh11G004360 vs. ExPASy Swiss-Prot
Match: Q945S8 (Histone-lysine N-methyltransferase ASHH3 OS=Arabidopsis thaliana OX=3702 GN=ASHH3 PE=2 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 2.5e-12
Identity = 30/50 (60.00%), Postives = 38/50 (76.00%), Query Frame = 0

Query: 83  VGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTK 133
           + GETR+G+FA R I+ GE LTY+Y+FVQFG +  CHCGA  C+R LG K
Sbjct: 208 IDGETRIGIFATRGIKKGEHLTYDYQFVQFGADQDCHCGAVGCRRKLGVK 257

BLAST of CmoCh11G004360 vs. ExPASy Swiss-Prot
Match: Q9M1X9 (Putative histone-lysine N-methyltransferase ASHH4 OS=Arabidopsis thaliana OX=3702 GN=ASHH4 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-10
Identity = 28/50 (56.00%), Postives = 37/50 (74.00%), Query Frame = 0

Query: 83  VGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTK 133
           + GETR+G+FA R I  GE LTY+Y+FVQFG +  C+CGA  C++ LG K
Sbjct: 203 IDGETRIGIFATRFINKGEQLTYDYQFVQFGADQDCYCGAVCCRKKLGAK 252

BLAST of CmoCh11G004360 vs. ExPASy Swiss-Prot
Match: Q4PBL3 (Histone-lysine N-methyltransferase, H3 lysine-36 specific OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=SET2 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.1e-07
Identity = 27/59 (45.76%), Postives = 36/59 (61.02%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEV-KCHCGAPNCQRYLGTK 133
           N  VS  QVG   R+G+FA R+I+ GE LT+ Y   ++G +  +C CG PNC   LG K
Sbjct: 330 NCAVSKWQVGKHLRMGIFAKRNIQKGEELTFNYNVDRYGNDAQECFCGEPNCVGTLGGK 388

BLAST of CmoCh11G004360 vs. ExPASy Swiss-Prot
Match: Q4WTT2 (Histone-lysine N-methyltransferase, H3 lysine-36 specific OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=set2 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 5.3e-07
Identity = 26/61 (42.62%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 74  NNLCVSDKQVGGE-TRVGVFAARSIEVGEPLTYEYRFVQFGPEVK-CHCGAPNCQRYLGT 133
           N  C  DK V GE  R+G+FA R+I+ GE L + Y   ++G + + C+CG PNC  ++G 
Sbjct: 296 NPNCYVDKWVVGEKLRMGIFAERAIQAGEELVFNYNVDRYGADPQPCYCGEPNCTGFIGG 355

BLAST of CmoCh11G004360 vs. ExPASy TrEMBL
Match: A0A6J1E212 (histone-lysine N-methyltransferase ASHR3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430113 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 1.1e-39
Identity = 89/122 (72.95%), Postives = 99/122 (81.15%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTAVYLVLPLALPALHLQQIPSSKDHKLQSPVTSI 194
           I+A+DLFWGTKRRRTSAA IT   S   ++L+LP  L  LHLQ IPSSK HKLQS V SI
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDSIS-LPLHLILPPVLLTLHLQLIPSSKVHKLQSEVPSI 537

Query: 195 KG 196
           +G
Sbjct: 538 QG 538

BLAST of CmoCh11G004360 vs. ExPASy TrEMBL
Match: A0A6J1JDD2 (histone-lysine N-methyltransferase ASHR3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485767 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.4e-39
Identity = 88/122 (72.13%), Postives = 99/122 (81.15%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTAVYLVLPLALPALHLQQIPSSKDHKLQSPVTSI 194
           I+A+DLFWGTKRRRTSAA IT   S   ++L+LP  L  LHLQ IPSSK HKLQS + SI
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDSIS-LPLHLILPPVLLTLHLQLIPSSKVHKLQSDIPSI 537

Query: 195 KG 196
           +G
Sbjct: 538 QG 538

BLAST of CmoCh11G004360 vs. ExPASy TrEMBL
Match: A0A6J1E322 (histone-lysine N-methyltransferase ASHR3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430113 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.0e-37
Identity = 88/139 (63.31%), Postives = 98/139 (70.50%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTA-----------------VYLVLPLALPALHLQ 194
           I+A+DLFWGTKRRRTSAA IT                        ++L+LP  L  LHLQ
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDDLESDVQSEDSFDSLYAYSISLPLHLILPPVLLTLHLQ 537

Query: 195 QIPSSKDHKLQSPVTSIKG 196
            IPSSK HKLQS V SI+G
Sbjct: 538 LIPSSKVHKLQSEVPSIQG 556

BLAST of CmoCh11G004360 vs. ExPASy TrEMBL
Match: A0A6J1JHJ5 (histone-lysine N-methyltransferase ASHR3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485767 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 5.1e-37
Identity = 87/139 (62.59%), Postives = 98/139 (70.50%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFS-----------------PTAVYLVLPLALPALHLQ 194
           I+A+DLFWGTKRRRTSAA IT                        ++L+LP  L  LHLQ
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDDLESDIQSEDLFDSLYAYSISLPLHLILPPVLLTLHLQ 537

Query: 195 QIPSSKDHKLQSPVTSIKG 196
            IPSSK HKLQS + SI+G
Sbjct: 538 LIPSSKVHKLQSDIPSIQG 556

BLAST of CmoCh11G004360 vs. ExPASy TrEMBL
Match: A0A0A0LW85 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G275930 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 2.2e-32
Identity = 71/82 (86.59%), Postives = 74/82 (90.24%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGAPNCQRYLGTKK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGAPNCQRYLGTKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITG 156
           ISA+DLFWGTKRRRTSAA ITG
Sbjct: 478 ISAIDLFWGTKRRRTSAARITG 499

BLAST of CmoCh11G004360 vs. NCBI nr
Match: KAG7035699.1 (Histone-lysine N-methyltransferase ASHR3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 211.5 bits (537), Expect = 9.9e-51
Identity = 112/146 (76.71%), Postives = 114/146 (78.08%), Query Frame = 0

Query: 14  LQMNARAQGGLTGLLYLFNGQHAVSVSDMEILHGHRKTVIIFFFLGQKMIIMLNHPILLK 73
           L MNARAQG LTGLLYLFNGQHAVSVSDME                          ILLK
Sbjct: 215 LIMNARAQGWLTGLLYLFNGQHAVSVSDME--------------------------ILLK 274

Query: 74  NNLCV-SDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTK 133
           NNL V SDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLG+K
Sbjct: 275 NNLRVSSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGSK 334

Query: 134 KISALDLFWGTKRRRTSAAHITGRFS 159
           K+SALDLFWGTKRRRTSAA ITGR S
Sbjct: 335 KVSALDLFWGTKRRRTSAAPITGRCS 334

BLAST of CmoCh11G004360 vs. NCBI nr
Match: KAG6587797.1 (Histone-lysine N-methyltransferase ASHR3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 179.9 bits (455), Expect = 3.2e-41
Identity = 94/125 (75.20%), Postives = 95/125 (76.00%), Query Frame = 0

Query: 19  RAQGGLTGLLYLFNGQHAVSVSDMEILHGHRKTVIIFFFLGQKMIIMLNHPILLKNNLCV 78
           +A G LTGLLYLFNGQHAVSVSDME                          ILLKNNL V
Sbjct: 41  KAYGWLTGLLYLFNGQHAVSVSDME--------------------------ILLKNNLRV 100

Query: 79  -SDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKKISAL 138
            SDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKKISAL
Sbjct: 101 SSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKKISAL 139

Query: 139 DLFWG 143
           DLFWG
Sbjct: 161 DLFWG 139

BLAST of CmoCh11G004360 vs. NCBI nr
Match: XP_022922047.1 (histone-lysine N-methyltransferase ASHR3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 173.7 bits (439), Expect = 2.3e-39
Identity = 89/122 (72.95%), Postives = 99/122 (81.15%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTAVYLVLPLALPALHLQQIPSSKDHKLQSPVTSI 194
           I+A+DLFWGTKRRRTSAA IT   S   ++L+LP  L  LHLQ IPSSK HKLQS V SI
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDSIS-LPLHLILPPVLLTLHLQLIPSSKVHKLQSEVPSI 537

Query: 195 KG 196
           +G
Sbjct: 538 QG 538

BLAST of CmoCh11G004360 vs. NCBI nr
Match: XP_022988562.1 (histone-lysine N-methyltransferase ASHR3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 173.3 bits (438), Expect = 3.0e-39
Identity = 88/122 (72.13%), Postives = 99/122 (81.15%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTAVYLVLPLALPALHLQQIPSSKDHKLQSPVTSI 194
           I+A+DLFWGTKRRRTSAA IT   S   ++L+LP  L  LHLQ IPSSK HKLQS + SI
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDSIS-LPLHLILPPVLLTLHLQLIPSSKVHKLQSDIPSI 537

Query: 195 KG 196
           +G
Sbjct: 538 QG 538

BLAST of CmoCh11G004360 vs. NCBI nr
Match: XP_022922046.1 (histone-lysine N-methyltransferase ASHR3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 165.6 bits (418), Expect = 6.2e-37
Identity = 88/139 (63.31%), Postives = 98/139 (70.50%), Query Frame = 0

Query: 75  NLCVSDKQVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTKK- 134
           N  +   QV GETRVGVFAARSIEVGEPLTY+YRFVQFGPEVKCHCGA NCQR+LG+KK 
Sbjct: 418 NCSLEKWQVEGETRVGVFAARSIEVGEPLTYDYRFVQFGPEVKCHCGASNCQRFLGSKKR 477

Query: 135 ISALDLFWGTKRRRTSAAHITGRFSPTA-----------------VYLVLPLALPALHLQ 194
           I+A+DLFWGTKRRRTSAA IT                        ++L+LP  L  LHLQ
Sbjct: 478 ITAMDLFWGTKRRRTSAARITDDLESDVQSEDSFDSLYAYSISLPLHLILPPVLLTLHLQ 537

Query: 195 QIPSSKDHKLQSPVTSIKG 196
            IPSSK HKLQS V SI+G
Sbjct: 538 LIPSSKVHKLQSEVPSIQG 556

BLAST of CmoCh11G004360 vs. TAIR 10
Match: AT4G30860.1 (SET domain group 4 )

HSP 1 Score: 111.7 bits (278), Expect = 9.9e-25
Identity = 57/85 (67.06%), Postives = 64/85 (75.29%), Query Frame = 0

Query: 66  LNHPILLKNNLCVSDK-QVGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPN 125
           LNH     N  CV +K QV GETRVGVFAAR IE GEPLTY+YRFVQFGPEVKC+CG+ N
Sbjct: 403 LNHSC---NPNCVLEKWQVEGETRVGVFAARQIEAGEPLTYDYRFVQFGPEVKCNCGSEN 462

Query: 126 CQRYLGTKK--ISALDLFWGTKRRR 148
           CQ YLGTK+   + L + WG KRRR
Sbjct: 463 CQGYLGTKRKEPNCLVVSWGAKRRR 484

BLAST of CmoCh11G004360 vs. TAIR 10
Match: AT2G44150.1 (histone-lysine N-methyltransferase ASHH3 )

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-13
Identity = 30/50 (60.00%), Postives = 38/50 (76.00%), Query Frame = 0

Query: 83  VGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTK 133
           + GETR+G+FA R I+ GE LTY+Y+FVQFG +  CHCGA  C+R LG K
Sbjct: 208 IDGETRIGIFATRGIKKGEHLTYDYQFVQFGADQDCHCGAVGCRRKLGVK 257

BLAST of CmoCh11G004360 vs. TAIR 10
Match: AT3G59960.1 (histone-lysine N-methyltransferase ASHH4 )

HSP 1 Score: 68.9 bits (167), Expect = 7.4e-12
Identity = 28/50 (56.00%), Postives = 37/50 (74.00%), Query Frame = 0

Query: 83  VGGETRVGVFAARSIEVGEPLTYEYRFVQFGPEVKCHCGAPNCQRYLGTK 133
           + GETR+G+FA R I  GE LTY+Y+FVQFG +  C+CGA  C++ LG K
Sbjct: 203 IDGETRIGIFATRFINKGEQLTYDYQFVQFGADQDCYCGAVCCRKKLGAK 252

BLAST of CmoCh11G004360 vs. TAIR 10
Match: AT1G76710.1 (SET domain group 26 )

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-06
Identity = 23/49 (46.94%), Postives = 29/49 (59.18%), Query Frame = 0

Query: 85  GETRVGVFAARSIEVGEPLTYEYRFVQF-GPEVKCHCGAPNCQRYLGTK 133
           GE RVG+FA  SI     L Y+Y F  + G +V+C CGA  C  +LG K
Sbjct: 183 GEVRVGIFAKESISPRTELAYDYNFEWYGGAKVRCLCGAVACSGFLGAK 231

BLAST of CmoCh11G004360 vs. TAIR 10
Match: AT1G76710.2 (SET domain group 26 )

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-06
Identity = 23/49 (46.94%), Postives = 29/49 (59.18%), Query Frame = 0

Query: 85  GETRVGVFAARSIEVGEPLTYEYRFVQF-GPEVKCHCGAPNCQRYLGTK 133
           GE RVG+FA  SI     L Y+Y F  + G +V+C CGA  C  +LG K
Sbjct: 183 GEVRVGIFAKESISPRTELAYDYNFEWYGGAKVRCLCGAVACSGFLGAK 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949T81.4e-2367.06Histone-lysine N-methyltransferase ASHR3 OS=Arabidopsis thaliana OX=3702 GN=ASHR... [more]
Q945S82.5e-1260.00Histone-lysine N-methyltransferase ASHH3 OS=Arabidopsis thaliana OX=3702 GN=ASHH... [more]
Q9M1X91.0e-1056.00Putative histone-lysine N-methyltransferase ASHH4 OS=Arabidopsis thaliana OX=370... [more]
Q4PBL33.1e-0745.76Histone-lysine N-methyltransferase, H3 lysine-36 specific OS=Ustilago maydis (st... [more]
Q4WTT25.3e-0742.62Histone-lysine N-methyltransferase, H3 lysine-36 specific OS=Neosartorya fumigat... [more]
Match NameE-valueIdentityDescription
A0A6J1E2121.1e-3972.95histone-lysine N-methyltransferase ASHR3-like isoform X2 OS=Cucurbita moschata O... [more]
A0A6J1JDD21.4e-3972.13histone-lysine N-methyltransferase ASHR3-like isoform X2 OS=Cucurbita maxima OX=... [more]
A0A6J1E3223.0e-3763.31histone-lysine N-methyltransferase ASHR3-like isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1JHJ55.1e-3762.59histone-lysine N-methyltransferase ASHR3-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A0A0LW852.2e-3286.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G275930 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG7035699.19.9e-5176.71Histone-lysine N-methyltransferase ASHR3, partial [Cucurbita argyrosperma subsp.... [more]
KAG6587797.13.2e-4175.20Histone-lysine N-methyltransferase ASHR3, partial [Cucurbita argyrosperma subsp.... [more]
XP_022922047.12.3e-3972.95histone-lysine N-methyltransferase ASHR3-like isoform X2 [Cucurbita moschata][more]
XP_022988562.13.0e-3972.13histone-lysine N-methyltransferase ASHR3-like isoform X2 [Cucurbita maxima][more]
XP_022922046.16.2e-3763.31histone-lysine N-methyltransferase ASHR3-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G30860.19.9e-2567.06SET domain group 4 [more]
AT2G44150.11.8e-1360.00histone-lysine N-methyltransferase ASHH3 [more]
AT3G59960.17.4e-1256.00histone-lysine N-methyltransferase ASHH4 [more]
AT1G76710.14.6e-0646.94SET domain group 26 [more]
AT1G76710.24.6e-0646.94SET domain group 26 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003616Post-SET domainSMARTSM00508PostSET_3coord: 114..130
e-value: 0.0014
score: 27.9
IPR003616Post-SET domainPROSITEPS50868POST_SETcoord: 114..130
score: 9.658456
NoneNo IPR availableGENE3D2.170.270.10SET domaincoord: 60..139
e-value: 5.2E-14
score: 54.3
NoneNo IPR availablePANTHERPTHR22884:SF494HISTONE-LYSINE N-METHYLTRANSFERASE ASHR3coord: 65..153
NoneNo IPR availablePANTHERPTHR22884SET DOMAIN PROTEINScoord: 65..153
NoneNo IPR availableSUPERFAMILY82199SET domaincoord: 67..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G004360.1CmoCh11G004360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010452 histone H3-K36 methylation
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0000785 chromatin
cellular_component GO:0005634 nucleus
molecular_function GO:0046975 histone methyltransferase activity (H3-K36 specific)
molecular_function GO:0046872 metal ion binding