CmoCh11G003400 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh11G003400 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 569/741 (76.79%), Postives = 648/741 (87.45%), Query Frame = 0

Query: 65  ASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVSATLC 124
           A  R +    ++ V E+     S+RF + +G P+PFG T RD G+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKP--LKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 125 LINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDP 184
           LI+L DL++ +V +EI LDP  NRTG VWHVFL+GDF DMLYGY+F GKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 185 SRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIY 244
           S ILLDPYAKA+ISR+EFG LGPDDNCWPQMA MVPT ++EFDWEGD+ L+ PQKDL+IY
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIY 223

Query: 245 EMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYNSVLG 304
           EMHVRGFTRHESS+  FPGTY G+ +KLD+LKELG+NCIELMPCHEFNELEYYSYN++LG
Sbjct: 224 EMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILG 283

Query: 305 DYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHT 364
           D++VNFWGYST+GF SPMIRY+SA   N    AINEFK+L+KEAHKRGIEVIMDVV NHT
Sbjct: 284 DHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHT 343

Query: 365 AEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRYWVIE 424
           AEGNE GPIFSFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR+FI+DCLRYWV E
Sbjct: 344 AEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTE 403

Query: 425 MHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGV 484
           MHVDGFRFDL SIM+R SSLWDA NVYG  +EGD LTTG+P+  PP+IDMISNDPIL+GV
Sbjct: 404 MHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGV 463

Query: 485 KLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 544
           KLIAEAWDAGGLYQVG FPHW +WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLY
Sbjct: 464 KLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLY 523

Query: 545 QGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 604
           Q GGRKPW+SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F S
Sbjct: 524 Q-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFAS 583

Query: 605 ISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEE 664
           ISVK+LRKRQMRNFFV LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE
Sbjct: 584 ISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEE 643

Query: 665 SSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSV 724
           + SDF RFCR++  FR ECESLGLNDFPTA+RLQWHG +P  P+WSE SRFVAF+LVDSV
Sbjct: 644 AHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSV 703

Query: 725 KREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFL 784
           K+EIY+AFN SHL   V+LP RPGY+WEP VDT K SP+D +T DL ER+ A++QY HFL
Sbjct: 704 KKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFL 763

Query: 785 DANLYPMLSYSSIILLLSPEK 806
           DAN+YPMLSYSSIILLLSP K
Sbjct: 764 DANVYPMLSYSSIILLLSPIK 781

BLAST of CmoCh11G003400 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 535/713 (75.04%), Postives = 601/713 (84.29%), Query Frame = 0

Query: 92  VFEGTPTPFGATARDGGINFAIYSGNAVSATLCLINLFDLQKKRVAQEITLDPLINRTGD 151
           V  G P P GATA DGG+NFA+YS  A +A+LCL    DL+   V +E+ LDPL NRTG+
Sbjct: 96  VLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGN 155

Query: 152 VWHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDPSRILLDPYAKAVISREEFGALGPDDNC 211
           VWHVF++G+  +MLYGY+F G F+P  G YFD S +++DPYAKAVISR E+G  GP  +C
Sbjct: 156 VWHVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDC 215

Query: 212 WPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDK 271
           WPQMAGM+P     FDW+GDLPL++PQKDL+IYEMH+RGFT+H SS    PGTY+G + K
Sbjct: 216 WPQMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVEHPGTYIGAISK 275

Query: 272 LDYLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIR 331
           LDYLKELGVNC+ELMPCHEFNELEY+S +S     K+NFWGYST+ F SPMIRYSS GIR
Sbjct: 276 LDYLKELGVNCVELMPCHEFNELEYFSCSS-----KMNFWGYSTINFFSPMIRYSSGGIR 335

Query: 332 NCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMG 391
           NCG+DAINEFK  ++EAHKRGIEVIMDVVFNHTAEGNE GPI SFRG+DN+ YYMLAP G
Sbjct: 336 NCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKG 395

Query: 392 EFYNYSGCGNTFNCNHPAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVY 451
           EFYNYSGCGNTFNCNHP VR FIVDCLRYWV EMHVDGFRFDLASIMTRG SLWD VNVY
Sbjct: 396 EFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVY 455

Query: 452 GNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEW 511
           G+P+EGD  TTG+PL  PPLIDMISNDPIL  VKLIAEAWDAGGLYQVG FPHW +WSEW
Sbjct: 456 GSPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEW 515

Query: 512 NGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVT 571
           NGKYRDIVRQFIKGTDGF+G FAECLCGSP+LYQ GGRKPW+SINF+CAHDGFTLADLVT
Sbjct: 516 NGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVT 575

Query: 572 YNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI 631
           YN K+N +NGEDN DGENHN SWNCG+EGEF  +SVK+LRKRQMRNFFV LMVSQGVPM 
Sbjct: 576 YNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMF 635

Query: 632 YMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDF 691
           YMGDEYGHTKGGNNNTYCHD+Y+NYFRWDK EE SSD  RFC LMT FR +CESLGL DF
Sbjct: 636 YMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEE-SSDLQRFCSLMTKFRKQCESLGLADF 695

Query: 692 PTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQW 751
           PTAQRL WHGH PG PDWSE SRFVAF+  D  K EIY+AFNASHLP  V LPERPGY+W
Sbjct: 696 PTAQRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERPGYRW 755

Query: 752 EPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSIILLLSPE 805
           EPLVDTGK +P+DFLT DL +R  A+  ++HFL++NLYPMLSYSSIIL L P+
Sbjct: 756 EPLVDTGKPAPYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILELQPD 802

BLAST of CmoCh11G003400 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 610.5 bits (1573), Expect = 2.7e-173
Identity = 334/722 (46.26%), Postives = 429/722 (59.42%), Query Frame = 0

Query: 56  SRINVSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYS 115
           S + V+  RA  R  E   S        ++ +  F+V  G  +P G +  D GINFA++S
Sbjct: 56  STLKVTCRRAHERVVEEEAST-------MTETKLFKVSSGEVSPLGVSQVDKGINFALFS 115

Query: 116 GNAVSATLCLINLFDLQKKRVAQ----EITLDPLINRTGDVWHVFLKG-DFTDMLYGYKF 175
            NA S TLCL +L    K         E+ LDP +N+TGD WH+ ++     ++LYGY+ 
Sbjct: 116 QNATSVTLCL-SLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRV 175

Query: 176 GGKFSPQEGCYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEG 235
            G    Q+G  FD S +LLDPYAK V     F   G     + Q  G        FDW  
Sbjct: 176 DGPGEWQQGHRFDRSILLLDPYAKLVKGHSSF---GDSSQKFAQFYGTYDFESSPFDWGD 235

Query: 236 DLPLQH-PQKDLIIYEMHVRGFTRHESS--QSGFPGTYLGLVDKLDYLKELGVNCIELMP 295
           D    + P+KDL+IYEM+VR FT  ESS       G+YLG ++K+ +L++LG+N +EL+P
Sbjct: 236 DYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLP 295

Query: 296 CHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKE 355
             EF+ELE     S   D+ VN WGYSTV F +PM RY+S         A  EFK ++K 
Sbjct: 296 VFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG--EGDPIKASKEFKEMVKA 355

Query: 356 AHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCN 415
            H  GIEVI+DVV+NHT E ++  P   SFRG+DN VYYML P  +  N+SGCGNT NCN
Sbjct: 356 LHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCN 415

Query: 416 HPAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPL 475
           HP V   I+D LR+WV E HVDGFRFDLAS++ R                    T GSPL
Sbjct: 416 HPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA-------------------TDGSPL 475

Query: 476 GNPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGT 535
             PPLI  I+ D +L   K+IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG 
Sbjct: 476 SAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGD 535

Query: 536 DGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNND 595
            G  G+FA  + GS +LYQ   RKP++ +NF+ AHDGFTL DLV+YN KHN ANGE  ND
Sbjct: 536 SGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGND 595

Query: 596 GENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNN 655
           G N N+SWNCG EGE     +K LR RQM+NF + LM+SQG PM+ MGDEYGHT+ GNNN
Sbjct: 596 GCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNN 655

Query: 656 TYCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGN 715
           +Y HD  +N F+W +++    +  RF   +  FR+    L   +F T   + WH     N
Sbjct: 656 SYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH---EDN 715

Query: 716 PDWSEASRFVAFTLVDSV-KREIYIAFNASHLPVTVTLPE-RPGYQWEPLVDTGKISPFD 767
            D SE S+F+AFTL D +  R+IY+AFNA    V   +P+  PG QW  + DT   SP D
Sbjct: 716 WDNSE-SKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDD 740

BLAST of CmoCh11G003400 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 9.0e-169
Identity = 336/758 (44.33%), Postives = 440/758 (58.05%), Query Frame = 0

Query: 59  NVSETRASSRGAEVRTSVTVAEEVEISNSNR-FEVFEGTPTPFGATARDGGINFAIYSGN 118
           +V  T A ++      S+T      +S++   F+   G   P G +  +GG+NFA++S +
Sbjct: 66  SVRSTTARAQNGNAGRSMTEERGCTMSDTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQH 125

Query: 119 AVSATLCLI---NLFDLQKKRVAQEITLDPLINRTGDVWHVFLKG-DFTDMLYGYKFGGK 178
           A S  LCL       + +K     E  LD   N+TGD+WHV ++G   + +LYGY+ GG 
Sbjct: 126 ASSVILCLKLPGRGTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGP 185

Query: 179 FSPQEGCYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLP 238
               +G  FD S +LLDPYAK V  R+ FG      +   Q  G        FDW  D  
Sbjct: 186 QGWDQGHRFDSSTVLLDPYAKLVSGRKYFGVAEEKSS---QHFGTYDFDSSPFDWGDDYR 245

Query: 239 LQH-PQKDLIIYEMHVRGFTRHESS--QSGFPGTYLGLVDKLDYLKELGVNCIELMPCHE 298
           L + P+ DL+IYEM+VR FT  ESS   S   G+YLGL+DK+ +L ELGVN +EL+P  E
Sbjct: 246 LPNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFE 305

Query: 299 FNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHK 358
           ++ELE+  Y +   D+ VN WGYST+ F +PM RY+SAG       A  E K ++KE HK
Sbjct: 306 YDELEFKRYPNP-RDHMVNTWGYSTINFFAPMSRYASAG--GGPVAASKELKQMVKELHK 365

Query: 359 RGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNNVYYM--LAPMGEFYNYSGCGNTFNCNH 418
            GIEVI+DVV+NHT E ++  P + SFRG+DN VYYM  L    E  N+SGCGNT NCNH
Sbjct: 366 AGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNH 425

Query: 419 PAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLG 478
           P V+  I+D LR+WV E H+DGFRFDLAS++ RG                     G PL 
Sbjct: 426 PVVKELILDSLRHWVEEYHIDGFRFDLASVLCRGPD-------------------GCPLD 485

Query: 479 NPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTD 538
            PPLI  I+ D +L   K+IAE WD GGLY VG FP+W  W+EWNGKYRD +R+FIKG  
Sbjct: 486 APPLIKEIAKDAVLSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDP 545

Query: 539 GFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDG 598
           G  G FA  + GS +LYQ   RKP++ +NF+ AHDGFTL DLV+YN KHN ANGE   DG
Sbjct: 546 GMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDG 605

Query: 599 ENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNT 658
            N N SWNCG EGE   ++V  LR RQM+NF V LM+SQG PM+ MGDEYGHT+ GNNN+
Sbjct: 606 CNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNS 665

Query: 659 YCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNP 718
           Y HD  IN F+W+++E+      RF   M  FR+    L  + F     + WH     N 
Sbjct: 666 YGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNPILRRDRFLNKNDVTWHEDCWEN- 725

Query: 719 DWSEASRFVAFTLVD-SVKREIYIAFNASHLPVTVTLPERPGYQ-WEPLVDTGKISPFDF 778
              + S+F+AFT+ D +   +IY+AFNA    V   +P  P ++ W  +VDT   SP D 
Sbjct: 726 ---QESKFLAFTVHDHNSGGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDI 782

Query: 779 LTSDLEERDVAIQQYAHFLDANLYPMLSYSSIILLLSP 804
           +   +                  Y +  YSSI+L   P
Sbjct: 786 VPEGVP------------FTGPKYRIAPYSSILLKAKP 782

BLAST of CmoCh11G003400 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 3.1e-129
Identity = 265/611 (43.37%), Postives = 346/611 (56.63%), Query Frame = 0

Query: 92  VFEGTPTPFGATARDGGINFAIYSGNAVSATLCLINLFDLQKKRVAQEITLDPLINRTGD 151
           V+ G   P GAT    G NF+++S  A    LCLI+     +  V   I LD +    G 
Sbjct: 19  VWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLID-----EDGVESRIPLDEV---DGY 78

Query: 152 VWHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDPSRILLDPYAKAVISREEFG-------- 211
           VWH +L        YG++  G F P  G   DPS++LLDPY K+      FG        
Sbjct: 79  VWHAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDV 138

Query: 212 -ALGPD------DNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIYEMHVRGFTR-HE 271
            A+ PD      D+    M  +V  ++  FDW  D   + P  + +IYE HV+G T+ H 
Sbjct: 139 NAVDPDSTPPMVDSLGHTMTSVV--INPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHP 198

Query: 272 SSQSGFPGTYLGLVDK--LDYLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKV-NFWG 331
           S      GTY GL     +D+L EL V  +ELMP H+F       ++S L D  + N+WG
Sbjct: 199 SIPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF------LHDSRLLDLGLRNYWG 258

Query: 332 YSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHTAEGNENGP 391
           Y+T GF +P  +Y+S   R  G  A+ EFK +++  H+ GIEVI+DVV+NHTAEGN  GP
Sbjct: 259 YNTFGFFAPHHQYAST--RQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGP 318

Query: 392 IFSFRGVDNNVYYMLAPMG-EFY-NYSGCGNTFNCNHPAVRRFIVDCLRYWVIEMHVDGF 451
             +FRG+DN  YY L      FY +++G GN+ N  HP   + I+D LRYWVIEMHVDGF
Sbjct: 319 TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGF 378

Query: 452 RFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEA 511
           RFDLAS + R     D ++ +                     D++  DP++  VKLIAE 
Sbjct: 379 RFDLASTLARELHDVDRLSAF--------------------FDLVQQDPVVSQVKLIAEP 438

Query: 512 WDAG-GLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGR 571
           WD G G YQVG FP   +W+EWNGKYRD VR + +G     G FA  L GS +LY+  GR
Sbjct: 439 WDVGEGGYQVGNFPG--LWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGR 498

Query: 572 KPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKK 631
           +P  SINF+ AHDGFTL DLV+YN KHN ANGE+N DGE++N SWNCG EG      +  
Sbjct: 499 RPSASINFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILA 558

Query: 632 LRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDF 681
           LR RQMRN +  LMVSQG PMI  GDE G T+ GNNN YC D+ +++  W  + + ++D 
Sbjct: 559 LRARQMRNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDW-SLVDKNADL 587

BLAST of CmoCh11G003400 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 1703.3 bits (4410), Expect = 0.0e+00
Identity = 809/809 (100.00%), Postives = 809/809 (100.00%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLLSPEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 809

BLAST of CmoCh11G003400 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 1680.2 bits (4350), Expect = 0.0e+00
Identity = 800/809 (98.89%), Postives = 802/809 (99.13%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSK NVIPIRRRSDIHLDL VDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVT+AEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPL NRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSG PGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYF WD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPER GY+WEPLVDTGKISPFDFLTSDLEERDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLL PEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLFPEKHAST 809

BLAST of CmoCh11G003400 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 716/808 (88.61%), Postives = 761/808 (94.18%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRS SL  S  FL    S FPE RRRSSK+N +  RRRSDIH+DL  +WI+SR+ +
Sbjct: 1   MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           S TRAS+RGAEV TSV +AEEVE+SN +RFEVFEG P P GATARDGG+NFA+YSGNAVS
Sbjct: 61  SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCL+NLFDLQ+ RV +E+TLDPL N+TGDVWHVFLKG FT+MLYGYKF G+FSPQ+GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISR EFGA GPD+NCWPQMAGMVPTV DEFDWEGDLPLQ+PQKD
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDEFDWEGDLPLQYPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESS S FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+SYN
Sbjct: 241 LIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGIR CGQDAINEFKLLI+EAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDN+VYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVS+SVKKLRKRQMRNFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSMSVKKLRKRQMRNFFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           K+EESSSDFHRFC L+TTFR+ECESLGLNDFPTA+RLQWHG+SPG PDWSEASRFVAFTL
Sbjct: 661 KLEESSSDFHRFCSLVTTFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVK EIYIAFNA+HLPVTVTLPERPGY+WEPLVDTGKISPFDFL+SDL ERDVAI+QY
Sbjct: 721 VDSVKGEIYIAFNANHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAS 809
           AHFLD NLYPML YSSIILLLSP + +S
Sbjct: 781 AHFLDNNLYPMLCYSSIILLLSPAEASS 808

BLAST of CmoCh11G003400 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 717/813 (88.19%), Postives = 755/813 (92.87%), Query Frame = 0

Query: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRIN 60
           MELVTRS ASL SS SFL  SN SFPE RRR+ K NV+  RRR+DI  DL  +WIHS +N
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  VSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120
               RA +RGAEV TSV VAEE EISN NRFEVF+GTPTPFGATARD GINFAIYS NAV
Sbjct: 61  ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180
           SATLCL++L DLQ+  V QEITLDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240
           CYFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPT +++   FDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240

Query: 241 PQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300
            QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVI 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420
           MDVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480
           CLRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKP NSINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660

Query: 661 FRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720
           FRWDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720

Query: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVA 780
           AFTLVDSVKRE+YIAFNASHLPVTVTLPERPGY+W+PLVDTGK SPFDFLT+D+ ER++A
Sbjct: 721 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLA 780

Query: 781 IQQYAHFLDANLYPMLSYSSIILLLSPEKHAST 810
           IQQYAHFLDANLYPMLSYSSIILLLSPE+  S+
Sbjct: 781 IQQYAHFLDANLYPMLSYSSIILLLSPEEPVSS 809

BLAST of CmoCh11G003400 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 717/813 (88.19%), Postives = 755/813 (92.87%), Query Frame = 0

Query: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRIN 60
           MELVTRS ASL SS SFL  SN SFPE RRR+ K NV+  RRR+DI  DL  +WIHS +N
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  VSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120
               RA +RGAEV TSV VAEE EISN NRFEVF+GTPTPFGATARD GINFAIYS NAV
Sbjct: 61  ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180
           SATLCL++L DLQ+  V QEITLDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240
           CYFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPT +++   FDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240

Query: 241 PQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300
            QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVI 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420
           MDVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480
           CLRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKP NSINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660

Query: 661 FRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720
           FRWDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720

Query: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVA 780
           AFTLVDSVKRE+YIAFNASHLPVTVTLPERPGY+W+PLVDTGK SPFDFLT+D+ ER++A
Sbjct: 721 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLA 780

Query: 781 IQQYAHFLDANLYPMLSYSSIILLLSPEKHAST 810
           IQQYAHFLDANLYPMLSYSSIILLLSPE+  S+
Sbjct: 781 IQQYAHFLDANLYPMLSYSSIILLLSPEEPVSS 809

BLAST of CmoCh11G003400 vs. NCBI nr
Match: XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1703.3 bits (4410), Expect = 0.0e+00
Identity = 809/809 (100.00%), Postives = 809/809 (100.00%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLLSPEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 809

BLAST of CmoCh11G003400 vs. NCBI nr
Match: KAG7021674.1 (Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1696.8 bits (4393), Expect = 0.0e+00
Identity = 806/809 (99.63%), Postives = 807/809 (99.75%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDL VDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFK LIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLE+RDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEKRDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLLSPEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 809

BLAST of CmoCh11G003400 vs. NCBI nr
Match: XP_023531773.1 (isoamylase 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1691.0 bits (4378), Expect = 0.0e+00
Identity = 803/809 (99.26%), Postives = 806/809 (99.63%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDL VDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSR AEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRRAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPL NRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           S+LGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKE+HKRGIEVIMDVV
Sbjct: 301 SLLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKESHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLR+RQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRERQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLLSPEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 809

BLAST of CmoCh11G003400 vs. NCBI nr
Match: KAG6587707.1 (Isoamylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1686.8 bits (4367), Expect = 0.0e+00
Identity = 805/821 (98.05%), Postives = 807/821 (98.29%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDL VDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFK LIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAP------------MGEFYNYSGCGNTFNCNHP 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAP            +GEFYNYSGCGNTFNCNHP
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPILLTLDGCFKFKLGEFYNYSGCGNTFNCNHP 420

Query: 421 AVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGN 480
           AVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGN
Sbjct: 421 AVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGN 480

Query: 481 PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDG 540
           PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDG
Sbjct: 481 PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDG 540

Query: 541 FSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE 600
           FSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE
Sbjct: 541 FSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE 600

Query: 601 NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTY 660
           NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTY
Sbjct: 601 NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTY 660

Query: 661 CHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPD 720
           CHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPD
Sbjct: 661 CHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPD 720

Query: 721 WSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTS 780
           WSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTS
Sbjct: 721 WSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTS 780

Query: 781 DLEERDVAIQQYAHFLDANLYPMLSYSSIILLLSPEKHAST 810
           DLE+RDVAIQQYAHFLDANLYPMLSYSSIILLLSPEKHAST
Sbjct: 781 DLEKRDVAIQQYAHFLDANLYPMLSYSSIILLLSPEKHAST 821

BLAST of CmoCh11G003400 vs. NCBI nr
Match: XP_022973965.1 (isoamylase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1680.2 bits (4350), Expect = 0.0e+00
Identity = 800/809 (98.89%), Postives = 802/809 (99.13%), Query Frame = 0

Query: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
           MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSK NVIPIRRRSDIHLDL VDWIHSRINV
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
           SETRASSRGAEVRTSVT+AEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS
Sbjct: 61  SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
           ATLCLINLFDLQKKRVAQEITLDPL NRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240
           YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQSG PGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360
           SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYF WD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWD 660

Query: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720
           KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780
           VDSVKREIYIAFNASHLPVTVTLPER GY+WEPLVDTGKISPFDFLTSDLEERDVAIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDANLYPMLSYSSIILLLSPEKHAST 810
           AHFLDANLYPMLSYSSIILLL PEKHAST
Sbjct: 781 AHFLDANLYPMLSYSSIILLLFPEKHAST 809

BLAST of CmoCh11G003400 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 569/741 (76.79%), Postives = 648/741 (87.45%), Query Frame = 0

Query: 65  ASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVSATLC 124
           A  R +    ++ V E+     S+RF + +G P+PFG T RD G+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKP--LKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 125 LINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDP 184
           LI+L DL++ +V +EI LDP  NRTG VWHVFL+GDF DMLYGY+F GKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 185 SRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIY 244
           S ILLDPYAKA+ISR+EFG LGPDDNCWPQMA MVPT ++EFDWEGD+ L+ PQKDL+IY
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIY 223

Query: 245 EMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYNSVLG 304
           EMHVRGFTRHESS+  FPGTY G+ +KLD+LKELG+NCIELMPCHEFNELEYYSYN++LG
Sbjct: 224 EMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILG 283

Query: 305 DYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHT 364
           D++VNFWGYST+GF SPMIRY+SA   N    AINEFK+L+KEAHKRGIEVIMDVV NHT
Sbjct: 284 DHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHT 343

Query: 365 AEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRYWVIE 424
           AEGNE GPIFSFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR+FI+DCLRYWV E
Sbjct: 344 AEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTE 403

Query: 425 MHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGV 484
           MHVDGFRFDL SIM+R SSLWDA NVYG  +EGD LTTG+P+  PP+IDMISNDPIL+GV
Sbjct: 404 MHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGV 463

Query: 485 KLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 544
           KLIAEAWDAGGLYQVG FPHW +WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLY
Sbjct: 464 KLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLY 523

Query: 545 QGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 604
           Q GGRKPW+SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F S
Sbjct: 524 Q-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFAS 583

Query: 605 ISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEE 664
           ISVK+LRKRQMRNFFV LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE
Sbjct: 584 ISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEE 643

Query: 665 SSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSV 724
           + SDF RFCR++  FR ECESLGLNDFPTA+RLQWHG +P  P+WSE SRFVAF+LVDSV
Sbjct: 644 AHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSV 703

Query: 725 KREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFL 784
           K+EIY+AFN SHL   V+LP RPGY+WEP VDT K SP+D +T DL ER+ A++QY HFL
Sbjct: 704 KKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFL 763

Query: 785 DANLYPMLSYSSIILLLSPEK 806
           DAN+YPMLSYSSIILLLSP K
Sbjct: 764 DANVYPMLSYSSIILLLSPIK 781

BLAST of CmoCh11G003400 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.9e-174
Identity = 334/722 (46.26%), Postives = 429/722 (59.42%), Query Frame = 0

Query: 56  SRINVSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYS 115
           S + V+  RA  R  E   S        ++ +  F+V  G  +P G +  D GINFA++S
Sbjct: 56  STLKVTCRRAHERVVEEEAST-------MTETKLFKVSSGEVSPLGVSQVDKGINFALFS 115

Query: 116 GNAVSATLCLINLFDLQKKRVAQ----EITLDPLINRTGDVWHVFLKG-DFTDMLYGYKF 175
            NA S TLCL +L    K         E+ LDP +N+TGD WH+ ++     ++LYGY+ 
Sbjct: 116 QNATSVTLCL-SLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRV 175

Query: 176 GGKFSPQEGCYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEG 235
            G    Q+G  FD S +LLDPYAK V     F   G     + Q  G        FDW  
Sbjct: 176 DGPGEWQQGHRFDRSILLLDPYAKLVKGHSSF---GDSSQKFAQFYGTYDFESSPFDWGD 235

Query: 236 DLPLQH-PQKDLIIYEMHVRGFTRHESS--QSGFPGTYLGLVDKLDYLKELGVNCIELMP 295
           D    + P+KDL+IYEM+VR FT  ESS       G+YLG ++K+ +L++LG+N +EL+P
Sbjct: 236 DYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLP 295

Query: 296 CHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKE 355
             EF+ELE     S   D+ VN WGYSTV F +PM RY+S         A  EFK ++K 
Sbjct: 296 VFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG--EGDPIKASKEFKEMVKA 355

Query: 356 AHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCN 415
            H  GIEVI+DVV+NHT E ++  P   SFRG+DN VYYML P  +  N+SGCGNT NCN
Sbjct: 356 LHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCN 415

Query: 416 HPAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPL 475
           HP V   I+D LR+WV E HVDGFRFDLAS++ R                    T GSPL
Sbjct: 416 HPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA-------------------TDGSPL 475

Query: 476 GNPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGT 535
             PPLI  I+ D +L   K+IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG 
Sbjct: 476 SAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGD 535

Query: 536 DGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNND 595
            G  G+FA  + GS +LYQ   RKP++ +NF+ AHDGFTL DLV+YN KHN ANGE  ND
Sbjct: 536 SGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGND 595

Query: 596 GENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNN 655
           G N N+SWNCG EGE     +K LR RQM+NF + LM+SQG PM+ MGDEYGHT+ GNNN
Sbjct: 596 GCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNN 655

Query: 656 TYCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGN 715
           +Y HD  +N F+W +++    +  RF   +  FR+    L   +F T   + WH     N
Sbjct: 656 SYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH---EDN 715

Query: 716 PDWSEASRFVAFTLVDSV-KREIYIAFNASHLPVTVTLPE-RPGYQWEPLVDTGKISPFD 767
            D SE S+F+AFTL D +  R+IY+AFNA    V   +P+  PG QW  + DT   SP D
Sbjct: 716 WDNSE-SKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDD 740

BLAST of CmoCh11G003400 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 322.4 bits (825), Expect = 1.0e-87
Identity = 217/692 (31.36%), Postives = 335/692 (48.41%), Query Frame = 0

Query: 95  GTPTPFGATA--RDGGINFAIYSGNAVSATLCLINLFDLQKKRVAQEITLDPLINRTGDV 154
           G P P G ++   D   NF+ +S ++ +  LCL +  D    + A E+ LDP +NRTGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 155 WHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDPSRILLDPYAKAV---ISREEFGALGPDD 214
           WH  +   +  + YGY+       +E    +   I+LDPYA  V   +S++  G+L    
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKSP 349

Query: 215 NCWPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQ--SGFPGTYLG 274
           +               FDW  D+    P + L++Y ++V+GFT+H SS+  S   GT+ G
Sbjct: 350 S---------------FDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSG 409

Query: 275 LVDKLDYLKELGVNCIELMPCHEFNELE--YYSYNSVLGDYKVNFWGYSTVGFLSPMIRY 334
           + +K+ +LK LG N + L P   F+E +  Y+ ++                 F SPM  Y
Sbjct: 410 VAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY 469

Query: 335 SSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYY 394
              G  N  + A+N  K+++K+ H  GIEV+++VVF HTA+        + RG+D++ YY
Sbjct: 470 ---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYY 529

Query: 395 MLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLW 454
                 +  + S      NCN+P V++ +++ LRYWV E HVDGF F  AS + RG    
Sbjct: 530 YKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG---- 589

Query: 455 DAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLY-QVGTFPH 514
                            G  L  PPL++ I+ DP+L   KLIA+ WD   +  +   FPH
Sbjct: 590 ---------------VHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPH 649

Query: 515 WCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGF 574
           W  W+E N +Y   VR F++G  G     A  +CGS +++   GR P  S N+I  + G 
Sbjct: 650 WKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGL 709

Query: 575 TLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMV 634
           +L D+V++ S   LA+            SWNCG+EG     +V + R +Q+RNF     +
Sbjct: 710 SLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYI 769

Query: 635 SQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEES-SSDFHRFCRLMTTFR-YE 694
           S GVP++ MGDE G +  G+            F W+ +  +  +   +F   MT+ R   
Sbjct: 770 SLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQITQFISFMTSVRARR 829

Query: 695 CESLGLNDFPTAQRLQWHGHSPGNPDWSE-ASRFVAFTLVDSVKRE-------------- 754
            +     DF   + + W+ +    P W + AS+F+A  +    + E              
Sbjct: 830 SDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSN 838

Query: 755 -IYIAFNASHLPVTVTLPERP-GYQWEPLVDT 758
            ++I FNAS  P +V LP  P G +W  LVDT
Sbjct: 890 DLFIGFNASDHPESVVLPSLPDGSKWRRLVDT 838

BLAST of CmoCh11G003400 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 322.4 bits (825), Expect = 1.0e-87
Identity = 217/692 (31.36%), Postives = 335/692 (48.41%), Query Frame = 0

Query: 95  GTPTPFGATA--RDGGINFAIYSGNAVSATLCLINLFDLQKKRVAQEITLDPLINRTGDV 154
           G P P G ++   D   NF+ +S ++ +  LCL +  D    + A E+ LDP +NRTGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 155 WHVFLKGDFTDMLYGYKFGGKFSPQEGCYFDPSRILLDPYAKAV---ISREEFGALGPDD 214
           WH  +   +  + YGY+       +E    +   I+LDPYA  V   +S++  G+L    
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKSP 349

Query: 215 NCWPQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQ--SGFPGTYLG 274
           +               FDW  D+    P + L++Y ++V+GFT+H SS+  S   GT+ G
Sbjct: 350 S---------------FDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSG 409

Query: 275 LVDKLDYLKELGVNCIELMPCHEFNELE--YYSYNSVLGDYKVNFWGYSTVGFLSPMIRY 334
           + +K+ +LK LG N + L P   F+E +  Y+ ++                 F SPM  Y
Sbjct: 410 VAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY 469

Query: 335 SSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYY 394
              G  N  + A+N  K+++K+ H  GIEV+++VVF HTA+        + RG+D++ YY
Sbjct: 470 ---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYY 529

Query: 395 MLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLW 454
                 +  + S      NCN+P V++ +++ LRYWV E HVDGF F  AS + RG    
Sbjct: 530 YKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG---- 589

Query: 455 DAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLY-QVGTFPH 514
                            G  L  PPL++ I+ DP+L   KLIA+ WD   +  +   FPH
Sbjct: 590 ---------------VHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPH 649

Query: 515 WCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGF 574
           W  W+E N +Y   VR F++G  G     A  +CGS +++   GR P  S N+I  + G 
Sbjct: 650 WKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGL 709

Query: 575 TLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMV 634
           +L D+V++ S   LA+            SWNCG+EG     +V + R +Q+RNF     +
Sbjct: 710 SLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYI 769

Query: 635 SQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEES-SSDFHRFCRLMTTFR-YE 694
           S GVP++ MGDE G +  G+            F W+ +  +  +   +F   MT+ R   
Sbjct: 770 SLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQITQFISFMTSVRARR 829

Query: 695 CESLGLNDFPTAQRLQWHGHSPGNPDWSE-ASRFVAFTLVDSVKRE-------------- 754
            +     DF   + + W+ +    P W + AS+F+A  +    + E              
Sbjct: 830 SDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSN 838

Query: 755 -IYIAFNASHLPVTVTLPERP-GYQWEPLVDT 758
            ++I FNAS  P +V LP  P G +W  LVDT
Sbjct: 890 DLFIGFNASDHPESVVLPSLPDGSKWRRLVDT 838

BLAST of CmoCh11G003400 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 84.3 bits (207), Expect = 4.8e-16
Identity = 96/376 (25.53%), Postives = 148/376 (39.36%), Query Frame = 0

Query: 99  PFGATARDGGINFAIYSGNAVSATLCLI-NLFDLQKKRVAQEITLDPLINRTGDVWHVFL 158
           P GA     G++  +++  A + ++C+  N  D     +       PL    G VW    
Sbjct: 207 PLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEIC------PLKEANG-VWSTEG 266

Query: 159 KGDFTDMLYGYK---FGGKFSPQEGCYFDPSRILLDPYAKAVIS--REEFGALGPDDNCW 218
              +    Y YK   +       E CY +      DPYA+ + +  R+ F      D+  
Sbjct: 267 ACSWGGCYYVYKVSVYHPSTMKLETCYAN------DPYARGLSADGRKTFLVNLDSDDLK 326

Query: 219 PQMAGMVPTVDDEFDWEGDLPLQHPQKDLIIYEMHVRGFTRH-ESSQSGFPGTYLGLVDK 278
           P+  G     D +       P      D+ IYE+HVR F+ + E+ +    G YL    K
Sbjct: 327 PE--GWDNLADKK-------PCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSK 386

Query: 279 -------LDYLKELGVNCIELMPCHEFNELEYYSYN------------------------ 338
                  L  L + G+  + L+P  +F +++    N                        
Sbjct: 387 DSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARIT 446

Query: 339 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 398
            +  D   N WGY+ V +  P   Y+S     C    I EF+ +++  +  G+ V++DVV
Sbjct: 447 EIQNDDGYN-WGYNPVLWGVPKGSYASDPTGPC---RIIEFRKMVQALNCTGLNVVLDVV 506

Query: 399 FNHTAEGNENGPIFSFRGVDNNV--YYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCL 435
           +NH    + +GP      +D  V  YY+      F   S C N     H  V R I D L
Sbjct: 507 YNHL---HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDL 553