CmoCh11G003210 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G003210
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Microsomal prostaglandin E synthase 2
Location	Cmo_Chr11: 1561022 .. 1563902 (-)
RNA-Seq Expression	CmoCh11G003210
Synteny	CmoCh11G003210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTTCCGCCCCAGGGGGCGGTCCTCCTATCTCCTCGATTCCATTGCCGGCGGCTCTGCCCGAGCTGCGTCGTTGGGCGTGGTTGAGGCTGTTGCTTCTCTAGCCGCTGCTGTTTCCATGTCCCAGGTACGAAGCGTGTCCGTTTTGTAATAAAGTTAAAGGTGAACCTTTAAATTTCTCGTTCTCGTTGATCTCAATCTTCTGATTAGTTTTGTTGGCGATGAATCTGTGTTAGTTTCGAGGGATTTTTGGAAGGAATTTTGGATTAGTAATTGCTGGGAAATTTACATTTTGGATTAGTAATCTTCTGATTGTACTTTTCAAATCCCTCCTCTGCGGTTGCATGCCCGTGTGCACTTAATGACGACGTTTTACTTGTAATCTCAGCTTTTCTCGACTACTACGATGTACCATACAAAGTTGTCGAGGTCAATCCATTCAGTAAGAAAGAAATTAAGTGGTGGTCGGAGTATAAGAAGGTGCCAATATTGGTGGTTGATGGCGAACAGCTGGTTGACTCCTCAGGTATTGTTTCATTCTTCTGAATGTTCTACTTCATATTGGAAATAGTAAATCTCTATCTGTATCACTAACTCCTGGCAATGGCATTGCTGCAGCCATAATCGACCAGTTGAGCCACAAGGCTTTGTTGGATAAGAATGCTTCTTCTGTCTCTGAAGATGATGAAGAAACTAAGTGGCGTAGGTAGGTTTTTGCTTCTGTATCTAAATCGCTTTTCCATGCAATTGAATTTCACATGTGAATTTTGTATTCAAGGGGGAGAGGAAGAGGGATTGACGATTGCTTATTAGGTTTGGCCACTTGATCTGTATGTTCATATGCTTGATTATAGATACAACATATGTGAAATTGTTTAGTGTTAAGATTCTGAAAATTTGTGTTAGAAAAGCAAAGGCTACGTATGTTCTGCGTCTTGTTGGGCTATTGGACAGTTCCATCTGGTTTAAACTTGAGCTAAAACCAGATGCATGGTGGTTTAGTAACTTGCAAGTCCATTTTTGGTTATTTTTTTGTGTGTGGTTGGGGGATGATAATTGAATTCTTATTCATTACTAGTATGAAACCTTTTGGTTTTTGATTTCTTTCATTTGTTTAAATTTAAATTGATGCAGATGGGTAGACAATCACTTGGTACATGTCTTATTCATTATTAGTATGAAATCACTTGCTAGTAGTGTTCTTCTTAACAAACAAATCTCCACGCTGATCGAGGATTTTCATTGGCATTTTTTGGTAGTCCGCTTCAGTAAGTCTTTACACCTCAAAGATTTCCATGTGATGCTAGGGAAATTTCGAAATTCTCCTTTATTTGGATAACAATTTATACTGTAGCTAGTTTCTGATTATGTTGGGAGATTAAACTCTTGATTTTGAGAGTTTAATTAGGATTTGATTCCATGTGAACGTCCAAGCCCACTGCCAGGCAGATATTGTCATCTTTGGGCTTTCCTTTCTTGGCTTCCCCTCAAGTTTTTTAAAACACATCTCGGAGAGTGGGACCCCCTAACAGTCCAAGTACTCGTTCTTTCTCCAATTGATGTGGGATCTCCAATCCACCTCCCTTTGGAGCCCAGCATCCTTATTAGTACACTGCCTTGTGTCCACCCCCTTTGAGACTCAGCCTCCTCGCTAGCACATCGCTCGGTATCTGGCTCTGATATCATTTGTAACATCCCAAGCCGACCACTAGTGGATATTGTTTTTTTGGGCTTTTCCTTTTAGCTTACTCTCAAGGTTTTTAAAACGCGTCTACTAGGGAGAGGTTTCCAGGCTTCGACGGTTACATTCTCATTCTCATTGTCACTTTATTTTGATTTTTTTGGGATCTAGATTGAAAATAAACTTAGTGATTCTTGAGTTTGTTAAGCTATATTTAATTCATCCGCTTCTCTTGTCAATTCTATTCTTAGTTAATTTTGGCTTTGCGGAGAAGACTATTGTAGAGTATGCTGGAGCCGCCGCTATGTCTTTCATATGAAAGAAATTAAAGAAGAAATATAATATTAGCGTTATACGAAGCAGCTTGGATGGACGGGACTCTCTAGGTATGTACTCTGTAGTGTTAACTGTCCTTTTATCTTTTTTTTCTCTTCTTTTCACCATTGGAGTTCGTTTTTTTAAGCATTAGTCTCTTTTCATGAAAAGTTTCATGTCTTGTTAAAGAGAAGAAAAGAAGAAAAGGAGGTTTCAGACATCAATTTAGGACTTAAACATGTAGTTGGTCTTTTAGAATCAATTGTCCTTCACATCAACGCTTAGAGATATGCCAAATTCACAACCTTCTGTTTGAAGTTCATGAATGGCTCTTGTTTTGCGTTTTTAGGAGGCTCAAAGCCTAATCTAGCTGATCTTGCGGTGTTCGGCGTATTAAGAAGACCCATACGTTACTTGAGGTCTGGGAAAGACATGGGGTGGAGAACACACGCATTGGTGTGAGTGGTACACCAAATGGAGAGCACTGTTGGAGACTCCTCCATGATCATATCCTAATCTCTCCTCTCATCAACTCTCATTTTCTGGGCGTTGGTTACCCTTTGTTCTTCTCTTTGTGGGTACATGTGATGTCCAACAACCATATTACTGCTGTCGAGCACCCCTTCTGGCATCGGAATGATGAATTTCAAACCCTTCTAAAAAATAAGACATGGTACTTAGTTCCTCCTGCTGCTAGTCTTAACCTTATTGATTGCAAATGAGTTTTCAAACTCAAGCAAAAGTCAGATGTCTTTATTGATCGCTACAAAACACGTCTAGTTGTTAAAGGGTTTAAACAATCGTATGACGTTGATTATGATGATACCTTCAGTCCAGTTGTTAAGCCCACTACCATTCGGCTTTTATTATCTCTTGCTATTTCTTGT

mRNA sequence

AGTTCCGCCCCAGGGGGCGGTCCTCCTATCTCCTCGATTCCATTGCCGGCGGCTCTGCCCGAGCTGCGTCGTTGGGCGTGGTTGAGGCTGTTGCTTCTCTAGCCGCTGCTGTTTCCATGTCCCAGCTTTTCTCGACTACTACGATGTACCATACAAAGTTGTCGAGGTCAATCCATTCAGTAAGAAAGAAATTAAGTGGTGGTCGGAGTATAAGAAGGTGCCAATATTGGTGGTTGATGGCGAACAGCTGGTTGACTCCTCAGCCATAATCGACCAGTTGAGCCACAAGGCTTTGTTGGATAAGAATGCTTCTTCTGTCTCTGAAGATGATGAAGAAACTAAGTGGCGTAGTTAATTTTGGCTTTGCGGAGAAGACTATTGTAGACTTGGATGGACGGGACTCTCTAGGAGGCTCAAAGCCTAATCTAGCTGATCTTGCGGTGTTCGGCGTATTAAGAAGACCCATACGTTACTTGAGGTCTGGGAAAGACATGGGGTGGAGAACACACGCATTGGTGTGAGTGGTACACCAAATGGAGAGCACTGTTGGAGACTCCTCCATGATCATATCCTAATCTCTCCTCTCATCAACTCTCATTTTCTGGGCGTTGGTTACCCTTTGTTCTTCTCTTTGTGGGTACATGTGATGTCCAACAACCATATTACTGCTGTCGAGCACCCCTTCTGGCATCGGAATGATGAATTTCAAACCCTTCTAAAAAATAAGACATGGTACTTAGTTCCTCCTGCTGCTAGTCTTAACCTTATTGATTGCAAATGAGTTTTCAAACTCAAGCAAAAGTCAGATGTCTTTATTGATCGCTACAAAACACGTCTAGTTGTTAAAGGGTTTAAACAATCGTATGACGTTGATTATGATGATACCTTCAGTCCAGTTGTTAAGCCCACTACCATTCGGCTTTTATTATCTCTTGCTATTTCTTGT

Coding sequence (CDS)

ATGCTTCTTCTGTCTCTGAAGATGATGAAGAAACTAAGTGGCGTAGTTAATTTTGGCTTTGCGGAGAAGACTATTGTAGACTTGGATGGACGGGACTCTCTAGGAGGCTCAAAGCCTAATCTAGCTGATCTTGCGGTGTTCGGCGTATTAAGAAGACCCATACGTTACTTGAGGTCTGGGAAAGACATGGGGTGGAGAACACACGCATTGGTGTGA

Protein sequence

MLLLSLKMMKKLSGVVNFGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDMGWRTHALV

Homology

[Close]

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL
Match: A0A2U1KPF0 (Microsomal prostaglandin E synthase 2 OS=Artemisia annua OX=35608 GN=CTI12_AA579410 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-09
Identity = 36/43 (83.72%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRD LGGSKPNLADLAVFGVL RPIRYLRSGKDM
Sbjct: 263 AETWVDALDGRDFLGGSKPNLADLAVFGVL-RPIRYLRSGKDM 304

[Close]

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL
Match: A0A6L2KXE2 (Microsomal prostaglandin E synthase 2 OS=Tanacetum cinerariifolium OX=118510 GN=Tci_025165 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-09
Identity = 36/43 (83.72%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRD LGGSKPNLADLAVFGVL RPIRYLRSGKDM
Sbjct: 264 AETWVDALDGRDFLGGSKPNLADLAVFGVL-RPIRYLRSGKDM 305

[Close]

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL
Match: A0A5E4F0W9 (Microsomal prostaglandin E synthase 2 OS=Prunus dulcis OX=3755 GN=ALMOND_2B029029 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.5e-09
Identity = 35/43 (81.40%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRD LGGSKPN+ADLAVFGVL RPIRYLRSGKDM
Sbjct: 266 AETWVNALDGRDFLGGSKPNMADLAVFGVL-RPIRYLRSGKDM 307

[Close]

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL
Match: A0A5N5MRI2 (Microsomal prostaglandin E synthase 2 OS=Salix brachista OX=2182728 GN=DKX38_008613 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.5e-09
Identity = 39/67 (58.21%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 7   KMMKKLSGVVN-----FGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGK 66
           K +KK   + +     +G AE  +  L GR  LGGSKPNLADLAVFGVL RPIRYL+SGK
Sbjct: 248 KKLKKRHNITDERAALYGAAETWVDALRGRQYLGGSKPNLADLAVFGVL-RPIRYLKSGK 307

Query: 67  DMGWRTH 69
           DM   TH
Sbjct: 308 DMVEHTH 313

[Close]

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL
Match: A0A6N2LE24 (Microsomal prostaglandin E synthase 2 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS213205 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.5e-09
Identity = 39/67 (58.21%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 7   KMMKKLSGVVN-----FGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGK 66
           K +KK   + +     +G AE  +  L GR  LGGSKPNLADLAVFGVL RPIRYL+SGK
Sbjct: 235 KKLKKRHNITDERAALYGAAETWVDALRGRQYLGGSKPNLADLAVFGVL-RPIRYLKSGK 294

Query: 67  DMGWRTH 69
           DM   TH
Sbjct: 295 DMVEHTH 300

[Close]

BLAST of CmoCh11G003210 vs. NCBI nr
Match: KAG6587689.1 (Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-11
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM
Sbjct: 105 AETWVDALDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 147

[Close]

BLAST of CmoCh11G003210 vs. NCBI nr
Match: GEU53187.1 (prostaglandin E synthase 2-like [Tanacetum cinerariifolium])

HSP 1 Score: 70.5 bits (171), Expect = 6.7e-09
Identity = 36/43 (83.72%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRD LGGSKPNLADLAVFGVL RPIRYLRSGKDM
Sbjct: 264 AETWVDALDGRDFLGGSKPNLADLAVFGVL-RPIRYLRSGKDM 305

[Close]

BLAST of CmoCh11G003210 vs. NCBI nr
Match: PWA38654.1 (Glutaredoxin [Artemisia annua])

HSP 1 Score: 70.5 bits (171), Expect = 6.7e-09
Identity = 36/43 (83.72%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 21  AEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGKDM 64
           AE  +  LDGRD LGGSKPNLADLAVFGVL RPIRYLRSGKDM
Sbjct: 263 AETWVDALDGRDFLGGSKPNLADLAVFGVL-RPIRYLRSGKDM 304

[Close]

BLAST of CmoCh11G003210 vs. NCBI nr
Match: KAB5557704.1 (hypothetical protein DKX38_008613 [Salix brachista])

HSP 1 Score: 69.7 bits (169), Expect = 1.1e-08
Identity = 39/67 (58.21%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 7   KMMKKLSGVVN-----FGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGK 66
           K +KK   + +     +G AE  +  L GR  LGGSKPNLADLAVFGVL RPIRYL+SGK
Sbjct: 248 KKLKKRHNITDERAALYGAAETWVDALRGRQYLGGSKPNLADLAVFGVL-RPIRYLKSGK 307

Query: 67  DMGWRTH 69
           DM   TH
Sbjct: 308 DMVEHTH 313

[Close]

BLAST of CmoCh11G003210 vs. NCBI nr
Match: KAG5246513.1 (prostaglandin synthase [Salix suchowensis])

HSP 1 Score: 69.7 bits (169), Expect = 1.1e-08
Identity = 39/67 (58.21%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 7   KMMKKLSGVVN-----FGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRYLRSGK 66
           K +KK   + +     +G AE  +  L GR  LGGSKPNLADLAVFGVL RPIRYL+SGK
Sbjct: 235 KKLKKRHNITDERAALYGAAETWVDALRGRQYLGGSKPNLADLAVFGVL-RPIRYLKSGK 294

Query: 67  DMGWRTH 69
           DM   TH
Sbjct: 295 DMVEHTH 300

[Close]

BLAST of CmoCh11G003210 vs. TAIR 10
Match: AT5G42150.1 (Glutathione S-transferase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-09
Identity = 37/67 (55.22%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 1   MLLLSLKMMKKLS----GVVNFGFAEKTIVDLDGRDSLGGSKPNLADLAVFGVLRRPIRY 60
           M  +S K+ KK +        +  AE  +  L  R  LGGSKPNL DLAVFGVL RPIRY
Sbjct: 225 MYFVSKKLKKKYNITDERAALYDAAETWVDALKERPYLGGSKPNLGDLAVFGVL-RPIRY 284

Query: 61  LRSGKDM 64
           LRSGKDM
Sbjct: 285 LRSGKDM 290

The following BLAST results are available for this feature:

BLAST of CmoCh11G003210 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh11G003210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2U1KPF0	3.3e-09	83.72	Microsomal prostaglandin E synthase 2 OS=Artemisia annua OX=35608 GN=CTI12_AA579...	[more]
A0A6L2KXE2	3.3e-09	83.72	Microsomal prostaglandin E synthase 2 OS=Tanacetum cinerariifolium OX=118510 GN=...	[more]
A0A5E4F0W9	5.5e-09	81.40	Microsomal prostaglandin E synthase 2 OS=Prunus dulcis OX=3755 GN=ALMOND_2B02902...	[more]
A0A5N5MRI2	5.5e-09	58.21	Microsomal prostaglandin E synthase 2 OS=Salix brachista OX=2182728 GN=DKX38_008...	[more]
A0A6N2LE24	5.5e-09	58.21	Microsomal prostaglandin E synthase 2 OS=Salix viminalis OX=40686 GN=SVIM_LOCUS2...	[more]

BLAST of CmoCh11G003210 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587689.1	1.4e-11	88.37	Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
GEU53187.1	6.7e-09	83.72	prostaglandin E synthase 2-like [Tanacetum cinerariifolium]	[more]
PWA38654.1	6.7e-09	83.72	Glutaredoxin [Artemisia annua]	[more]
KAB5557704.1	1.1e-08	58.21	hypothetical protein DKX38_008613 [Salix brachista]	[more]
KAG5246513.1	1.1e-08	58.21	prostaglandin synthase [Salix suchowensis]	[more]

BLAST of CmoCh11G003210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42150.1	1.4e-09	55.22	Glutathione S-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1050.10		coord: 5..69 e-value: 1.3E-6 score: 30.0
None	No IPR available	PANTHER	PTHR12782	MICROSOMAL PROSTAGLANDIN E SYNTHASE-2	coord: 21..67
None	No IPR available	PANTHER	PTHR12782:SF12	PROSTAGLANDIN E SYNTHASE 2-RELATED	coord: 21..67
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 31..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G003210.1	CmoCh11G003210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098869	cellular oxidant detoxification
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0001516	prostaglandin biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0036134	12-hydroxyheptadecatrienoic acid synthase activity
molecular_function	GO:0004362	glutathione-disulfide reductase (NADPH) activity
molecular_function	GO:0097573	glutathione oxidoreductase activity
molecular_function	GO:0050220	prostaglandin-E synthase activity