CmoCh11G002460 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G002460
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPhosphopyruvate hydratase
LocationCmo_Chr11: 1222661 .. 1225938 (-)
RNA-Seq ExpressionCmoCh11G002460
SyntenyCmoCh11G002460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCATTTCCATTTCCATTTCCATTTCCATTTCTCACCAACCTCATTGCTCTCAATCGATAATTTCCATGGCTTCTCTTCCTTCCCCACCTACCACTACCACGAATCTCCTCAACAAATCCCTTCTTCCTTCCAAGCCTCAATCTCCGATCATCTCAATCCCTTCTTCTTCCTCCTCGAAGCCTCTTCGTTCACATGGCGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCTTCGACCAAGGCGTCCAGCGAATTTAAGTTGAAATCTGTCAAGGCCCGGCAGATCATTGACAGCCGAGGCAATCCGACTGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGCGGAGCCTCCACTGGGATCTATGAGGCCTTGGAGCTTAGAGATGGGGATAAAAACGTTTATGGCGGTAAAGGCGTGCTTACCGCTGTTAAGAATATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGACGTCAGGTGGATTTAATCTTTTTTTTTTTTTTTTTCTTTATTTGCTTTAACGAACATCGGTTGAAATGTTCATAATAGTAAGTGAAATCTTGCAATCTGAACAAATTGTTTCTTCTACTTTCTGGAAAATTAGGAACATAATTCTCCTCTCCGAGTGTTTTACTGTTTTTGGTCCGACTTTTTCTTCTTGAAACCTTGTTTGATAGTTTACTGCAAATTTTTTTTCACATTTACGACTTCTTGTTAGGACTATAATTTGCGTAGTTTCAGATTTCTGTCTGAAACTTCAAGTACTTCTGCAAATGGTGGCTTAGTTCATGCTGGGATTGACAATCTATATGTGTTTTCGTTGGGCTTACTAATATCGTTTGCAGCCTTGTTATTCAAATTGTTACTGAAGAAGCGAAATTCCAAGTTTTTTTGGTCTGACAATGTGGAAGTTGTTTCATTTCTGTAGAAATCAAGAAGATGTGGATGCAATTATGCTGGAAATTGATGGTACCACAAACAAGTCGAAACTTGGTGCAAATGCTATACTGGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGACACAAAGGAACTTGTTATGCCAGTCCCTGCTTTTAATGTTATAAATGGAGGTAGCCATGCTGGAAATAATCTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTGCATCTTTCGCGGAGGCGCTTCGCATGGGAAGTGAAGGTTTGCAGCTTCTTTTTAAGCACTGCAAATTTATTGTTAACATAGACAGTTTCTTCGTCTTCATCATTTGCATATACTTAGCTTAGTCATTAATGAGTAGCCCGATTTTATTGTTTTCTTATGAACAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTTGCTCCAAATGTTCAAGATAACAGAGAGGGATTGGTTTTGCTTACTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGGTATATAATACTAAATTCTCCCTTTTGTCTTGGAATGCCATTGAAAGGGCCGGTTATAAGGCAACATTTATTTTGATATACAGATCAAGATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAGATATGATCTGAACTTCAAGAAACAGCCCAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGTGAACTCTATAAACAGTTTGTGAATGATTTTCCGATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGCTCCTGGGCTTCCCTACAATCTTCTGTCGGTGTGCAAATTGTGGGAGATGACTTGCTGGTAACAAATCCTAAGAGAATTGCTGAAGCCATACAGAAGAAGGCTTGTAATGCTTTACTTCTGAAGGTAAGCAACCTCTTATTTCCTTCTTTCCTTTCTTTTCTGGCTGAACAGTGTCCATTAGAATATGTCATTTTGTGAGATCCCTCATCGGTAGTTAGAATATGTCATTTGGTGAGACTCCTCGTCAGTAGTTAGAATATGTCATTTTGTGAGACCCCTCATCGGAGTTAGAATATGTCATTTTGTGAGATCTTATGTCAGTTGAAAAGGGGAATAAAGTATTTCTTATAAGGGTGTGAAAACCTTTTCCTAGTAGATGCGTTTTAAAACTGTGAGGCTAACGGCGATACGTAACCGGCCAAAGCGGACAATATGTACTAGCGGTGGGCTTGGGCTGTTATAAATGGTATCAGAGTCAAACACCGGGCGGTGTGCCAGCGAGTATTCTAGCCTCCAAAGGGGGTGAATTGTGAGATCCCACATTAGTTGGAGAGGAAAATGAAATATTTTTTATAAGGGTGTAGAAATCTTTCCCTAGTAGACGCGTTTTAAAACCGTGAGGCTGACGGCGCTACGTAACGGACCAATGTAGACAATATCTACTAGTGGTGGTTTGAGCTGAGCTGTTCCACATTTTGATCTGCAATTTGCTCATATTTAGACATTTCTTCTGCAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTCGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGTAGTGGTGAAACTGAAGATAATTTTATTGCCGATCTTTCTGTTGGCTTAGCCAGTGGACAGGTTCTATTCATCTTCAGCTCTTCCATCAATAACTATTAAGTCGACATAACATTCATAACTGAATATTTTGTTACTCTTCTATAGATCAAAACCGGAGCTCCATGCCGAAGTGAACGGTTAGCCAAATATAATCAGGTAACTGTGATCAAACCACAACTTCAAAAAATTATTGTCCTTGTTGAATGTTCCAACTGTTCTTGATGATGGCTATTCCTTGAAATGTTTGTTTCGCTAATTGTGCTGAAATAGCCACAACAGTTCTAATTCTTCTCGATATGTTTGAAAATTTGGATGCAGCTTCTTCGCATTGAAGAAGAACTTGGGAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAGGAGGTAAAAGTTTAAGGCGACAGTGATAATGTATCAATTTTAGGCTGAAGATTTGGTTGATAGAAGAAAATGTAGAGGATTTCTGCGGAAACAATATGCAGAACTATCCTGTCTGAGATGTTATCATGAGTACTTCAATTTTTGTTTTGAACTATCCATGCAAATTTTCGTTTCGTTTCAAAATAATGCAAGCTCTTTTCTGATGCAAGCTAC

mRNA sequence

TTTCCATTTCCATTTCCATTTCCATTTCCATTTCTCACCAACCTCATTGCTCTCAATCGATAATTTCCATGGCTTCTCTTCCTTCCCCACCTACCACTACCACGAATCTCCTCAACAAATCCCTTCTTCCTTCCAAGCCTCAATCTCCGATCATCTCAATCCCTTCTTCTTCCTCCTCGAAGCCTCTTCGTTCACATGGCGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCTTCGACCAAGGCGTCCAGCGAATTTAAGTTGAAATCTGTCAAGGCCCGGCAGATCATTGACAGCCGAGGCAATCCGACTGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGCGGAGCCTCCACTGGGATCTATGAGGCCTTGGAGCTTAGAGATGGGGATAAAAACGTTTATGGCGGTAAAGGCGTGCTTACCGCTGTTAAGAATATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGACGTCAGAAATCAAGAAGATGTGGATGCAATTATGCTGGAAATTGATGGTACCACAAACAAGTCGAAACTTGGTGCAAATGCTATACTGGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGACACAAAGGAACTTGTTATGCCAGTCCCTGCTTTTAATGTTATAAATGGAGGTAGCCATGCTGGAAATAATCTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTGCATCTTTCGCGGAGGCGCTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTTGCTCCAAATGTTCAAGATAACAGAGAGGGATTGGTTTTGCTTACTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAGATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAGATATGATCTGAACTTCAAGAAACAGCCCAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGTGAACTCTATAAACAGTTTGTGAATGATTTTCCGATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGCTCCTGGGCTTCCCTACAATCTTCTGTCGGTGTGCAAATTGTGGGAGATGACTTGCTGGTAACAAATCCTAAGAGAATTGCTGAAGCCATACAGAAGAAGGCTTGTAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTCGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGTAGTGGTGAAACTGAAGATAATTTTATTGCCGATCTTTCTGTTGGCTTAGCCAGTGGACAGATCAAAACCGGAGCTCCATGCCGAAGTGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGGAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAGGAGGTAAAAGTTTAAGGCGACAGTGATAATGTATCAATTTTAGGCTGAAGATTTGGTTGATAGAAGAAAATGTAGAGGATTTCTGCGGAAACAATATGCAGAACTATCCTGTCTGAGATGTTATCATGAGTACTTCAATTTTTGTTTTGAACTATCCATGCAAATTTTCGTTTCGTTTCAAAATAATGCAAGCTCTTTTCTGATGCAAGCTAC

Coding sequence (CDS)

ATGGCTTCTCTTCCTTCCCCACCTACCACTACCACGAATCTCCTCAACAAATCCCTTCTTCCTTCCAAGCCTCAATCTCCGATCATCTCAATCCCTTCTTCTTCCTCCTCGAAGCCTCTTCGTTCACATGGCGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCTTCGACCAAGGCGTCCAGCGAATTTAAGTTGAAATCTGTCAAGGCCCGGCAGATCATTGACAGCCGAGGCAATCCGACTGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGCGGAGCCTCCACTGGGATCTATGAGGCCTTGGAGCTTAGAGATGGGGATAAAAACGTTTATGGCGGTAAAGGCGTGCTTACCGCTGTTAAGAATATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGACGTCAGAAATCAAGAAGATGTGGATGCAATTATGCTGGAAATTGATGGTACCACAAACAAGTCGAAACTTGGTGCAAATGCTATACTGGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGACACAAAGGAACTTGTTATGCCAGTCCCTGCTTTTAATGTTATAAATGGAGGTAGCCATGCTGGAAATAATCTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTGCATCTTTCGCGGAGGCGCTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTTGCTCCAAATGTTCAAGATAACAGAGAGGGATTGGTTTTGCTTACTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAGATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAGATATGATCTGAACTTCAAGAAACAGCCCAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGTGAACTCTATAAACAGTTTGTGAATGATTTTCCGATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGCTCCTGGGCTTCCCTACAATCTTCTGTCGGTGTGCAAATTGTGGGAGATGACTTGCTGGTAACAAATCCTAAGAGAATTGCTGAAGCCATACAGAAGAAGGCTTGTAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTCGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGTAGTGGTGAAACTGAAGATAATTTTATTGCCGATCTTTCTGTTGGCTTAGCCAGTGGACAGATCAAAACCGGAGCTCCATGCCGAAGTGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGGAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAG

Protein sequence

MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGNVRYAGESFRSP
Homology
BLAST of CmoCh11G002460 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.4e-225
Identity = 399/483 (82.61%), Postives = 432/483 (89.44%), Query Frame = 0

Query: 9   TTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKASSEFKLKSV 68
           TT  + L +S L     S    + S+ S    R  GV+CSV          + E ++K V
Sbjct: 4   TTKPHHLQRSFLSPSRVSGERYLESAPSCLRFRRSGVQCSV---------VAKECRVKGV 63

Query: 69  KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGGKGVLTAVK 128
           KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+K
Sbjct: 64  KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIK 123

Query: 129 NINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLP 188
           NIN+++APKL+GVDVRNQ DVDA+MLE+DGT NKSKLGANAILGVSLSVCRAGAGAKG+P
Sbjct: 124 NINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVP 183

Query: 189 LYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEALRMGSEVY 248
           LY+HIQE S TKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGA SF+EA +MGSEVY
Sbjct: 184 LYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVY 243

Query: 249 HTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIKIGMDVAAS 308
           HTLKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIKIGMDVAAS
Sbjct: 244 HTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAAS 303

Query: 309 EFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQDDWSSWASL 368
           EFF KDGRYDLNFKKQPNDGAHV SA  L +LY++F+ DFPIVSIEDPFDQDDWSSWASL
Sbjct: 304 EFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASL 363

Query: 369 QSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGV 428
           QSSV +Q+VGDDLLVTNPKRIAEAI+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGV
Sbjct: 364 QSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGV 423

Query: 429 MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGNVRYAGESF 488
           MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELGNVRYAGE+F
Sbjct: 424 MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAF 477

Query: 489 RSP 492
           RSP
Sbjct: 484 RSP 477

BLAST of CmoCh11G002460 vs. ExPASy Swiss-Prot
Match: P42894 (Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 7.1e-169
Identity = 305/434 (70.28%), Postives = 359/434 (82.72%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKNVYGGKGV 124
           +  V ARQI DSRGNPTVEV++ TD  L+R+AVPSGASTG++EALELRDG K  Y GKGV
Sbjct: 3   ITKVHARQIFDSRGNPTVEVEVTTDKGLFRAAVPSGASTGVHEALELRDGIKADYVGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAG 184
           L AV+N+N  +AP LV   +DV+NQ+ VD  +L++DGT NKSKLGANAILGVSL+V RAG
Sbjct: 63  LKAVENVNKTIAPALVAANLDVKNQKAVDDFLLKLDGTPNKSKLGANAILGVSLAVARAG 122

Query: 185 AGAKGLPLYRHIQELSDTK-ELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 244
           A  KG+PLY+H+ EL+  K   ++PVP+ NV+NGGSHAGN LAMQEFMILP GA SF EA
Sbjct: 123 AADKGVPLYQHLGELAGNKGPWILPVPSMNVLNGGSHAGNKLAMQEFMILPTGAKSFTEA 182

Query: 245 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 304
           L+MGSEVYH LK +IKAKYGQDACNVGDEGGFAPN+QDN+EGL LL +AI KAGYTGK+K
Sbjct: 183 LKMGSEVYHALKSVIKAKYGQDACNVGDEGGFAPNIQDNKEGLELLNEAIAKAGYTGKVK 242

Query: 305 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 364
           IGMDVA+SEF+ KDG+YDL+FK   +D +   S   LG+ YK+  +++PIVSIEDP+DQD
Sbjct: 243 IGMDVASSEFY-KDGKYDLDFKNPNSDPSKWISGEELGQFYKEITSEYPIVSIEDPYDQD 302

Query: 365 DWSSWASLQSSV--GVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAA 424
           D+ SW+  ++ +   +QIVGDDL VTNPKRIA AI+KKACN LLLKVNQIG+V+ESIQAA
Sbjct: 303 DFESWSKFRADMQDKIQIVGDDLTVTNPKRIAMAIEKKACNGLLLKVNQIGTVSESIQAA 362

Query: 425 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 484
           LD+   GWGVMVSHRSGETED FIADL VGL SGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 363 LDAFNDGWGVMVSHRSGETEDTFIADLVVGLKSGQIKTGAPCRSERLAKYNQLLRIEEEL 422

Query: 485 G-NVRYAGESFRSP 492
           G N  YAGE+FR P
Sbjct: 423 GANATYAGENFRRP 435

BLAST of CmoCh11G002460 vs. ExPASy Swiss-Prot
Match: P26300 (Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 2.7e-168
Identity = 307/438 (70.09%), Postives = 360/438 (82.19%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDL-ITDDLY-RSAVPSGASTGIYEALELRDGDKNVYGGKG 124
           +KS+KARQI DSRGNPTVEVD+ I++ ++ R+AVPSGASTGIYEALELRDG  + Y GKG
Sbjct: 4   IKSIKARQIFDSRGNPTVEVDVHISNGVFARAAVPSGASTGIYEALELRDGGSD-YLGKG 63

Query: 125 VLTAVKNINDILAPKLVGVDVRNQEDVDAIML-EIDGTTN-----KSKLGANAILGVSLS 184
           V  AV N+N I+ P LVG D  +Q  +D  M+ ++DGT N     K KLGANAIL VSL+
Sbjct: 64  VSKAVNNVNSIIGPALVGKDPTDQTGLDNFMVHQLDGTQNEWGWCKEKLGANAILAVSLA 123

Query: 185 VCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAAS 244
           VC+AGA  + +PLY+HI +L+  K+LV+PVPAFNVINGGSHAGN LAMQEFMILPVGAA+
Sbjct: 124 VCKAGAAVRNVPLYKHIADLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAAN 183

Query: 245 FAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYT 304
           F EA++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYT
Sbjct: 184 FKEAMKMGCEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYT 243

Query: 305 GKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDP 364
           GK+ IGMDVAASEF+ KD  YDLNFK++ NDG+   S   L +LYK FV+++PIVSIEDP
Sbjct: 244 GKVVIGMDVAASEFYGKDKSYDLNFKEESNDGSQKISGDQLKDLYKSFVSEYPIVSIEDP 303

Query: 365 FDQDDWSSWASLQSSVG--VQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTES 424
           FDQDDW ++A L + +G  VQIVGDDLLVTNPKR+A+AI +K CNALLLKVNQIGSVTES
Sbjct: 304 FDQDDWETYAKLTAEIGEQVQIVGDDLLVTNPKRVAKAIAEKTCNALLLKVNQIGSVTES 363

Query: 425 IQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRI 484
           I+A   SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRI
Sbjct: 364 IEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRI 423

Query: 485 EEELGN-VRYAGESFRSP 492
           EEELG+   YAG SFR P
Sbjct: 424 EEELGSEAVYAGASFRKP 440

BLAST of CmoCh11G002460 vs. ExPASy Swiss-Prot
Match: P09104 (Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3)

HSP 1 Score: 592.4 bits (1526), Expect = 4.6e-168
Identity = 297/431 (68.91%), Postives = 357/431 (82.83%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLIT-DDLYRSAVPSGASTGIYEALELRDGDKNVYGGKGV 124
           ++ + AR+I+DSRGNPTVEVDL T   L+R+AVPSGASTGIYEALELRDGDK  Y GKGV
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAG 184
           L AV +IN  +AP L+  G+ V  QE +D +MLE+DGT NKSK GANAILGVSL+VC+AG
Sbjct: 63  LKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 185 AGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEAL 244
           A  + LPLYRHI +L+   +L++PVPAFNVINGGSHAGN LAMQEFMILPVGA SF +A+
Sbjct: 123 AAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 245 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIKI 304
           R+G+EVYHTLKG+IK KYG+DA NVGDEGGFAPN+ +N E L L+ +AI+KAGYT KI I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVI 242

Query: 305 GMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQDD 364
           GMDVAASEF+ +DG+YDL+F K P D +   +   LG LY+ FV D+P+VSIEDPFDQDD
Sbjct: 243 GMDVAASEFY-RDGKYDLDF-KSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDD 302

Query: 365 WSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDS 424
           W++W+   ++VG+QIVGDDL VTNPKRI  A+++KACN LLLKVNQIGSVTE+IQA   +
Sbjct: 303 WAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLA 362

Query: 425 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN- 484
           +  GWGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RIEEELG+ 
Sbjct: 363 QENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDE 422

Query: 485 VRYAGESFRSP 492
            R+AG +FR+P
Sbjct: 423 ARFAGHNFRNP 431

BLAST of CmoCh11G002460 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 1.0e-167
Identity = 299/431 (69.37%), Postives = 355/431 (82.37%), Query Frame = 0

Query: 65  LKSVKARQIIDSRGNPTVEVDLITD-----DLYRSAVPSGASTGIYEALELRDGDKNVYG 124
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDK+ Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 125 GKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCR 184
           GKGVL A+KNI +++ P ++G  V +Q  +D +M+++DGT NK KLGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 185 AGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAE 244
           AGA  + LPLY++I E++ TK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 245 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKI 304
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 305 KIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQ 364
           KIGMD AASEF  ++G YDL+FK + NDG+ V S   LG+LY++F+ ++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASEFKVENG-YDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 365 DDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAAL 424
           DDW S+  L +SV +QIVGDDLLVTNP+RI   I+KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 425 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 484
           DSK A WGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 485 -NVRYAGESFR 490
            N  YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

BLAST of CmoCh11G002460 vs. ExPASy TrEMBL
Match: A0A6J1EV05 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.5e-275
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
           SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG
Sbjct: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. ExPASy TrEMBL
Match: A0A6J1HM99 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111465484 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.5e-273
Identity = 486/491 (98.98%), Postives = 487/491 (99.19%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVA APS STKAS
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAAAPSVSTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
            EFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 61  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL KYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLTKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.3e-252
Identity = 458/498 (91.97%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP-------SSSSSKPLRSHGVRCSVAVAP 60
           MASLPSPP  T NLLNK L  S PQS I+SIP       SSSSSKPLRS  +RCSVAVAP
Sbjct: 1   MASLPSPP--TMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAP 60

Query: 61  SASTKASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120
           S ST  S EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 61  SVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120

Query: 121 DKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180
           DK VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGV
Sbjct: 121 DKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGV 180

Query: 181 SLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240
           SLSVCRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 181 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240

Query: 241 AASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKA 300
           A+SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKA
Sbjct: 241 ASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 300

Query: 301 GYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSI 360
           GYTGKIKIGMDVAASEFFTK G+YDLNFKKQPNDGAHVHSAH LGELYKQFV DFPIVSI
Sbjct: 301 GYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSI 360

Query: 361 EDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTE 420
           EDPFDQDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTE
Sbjct: 361 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTE 420

Query: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR
Sbjct: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480

Query: 481 IEEELGNVRYAGESFRSP 492
           IEEELG+VRYAG SFRSP
Sbjct: 481 IEEELGSVRYAGASFRSP 496

BLAST of CmoCh11G002460 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 457/498 (91.77%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP-------SSSSSKPLRSHGVRCSVAVAP 60
           MASLPSPP  T NLLNK L  S PQS I+SIP       SSSSSKPLRS  +RCSVAVAP
Sbjct: 1   MASLPSPP--TMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAP 60

Query: 61  SASTKASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120
           S ST  S EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 61  SVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120

Query: 121 DKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180
           DK VYGGKGVLTAVKNI DILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGV
Sbjct: 121 DKTVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGV 180

Query: 181 SLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240
           SLSVCRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 181 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240

Query: 241 AASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKA 300
           A+SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKA
Sbjct: 241 ASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 300

Query: 301 GYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSI 360
           GYTGKIKIGMDVAASEFFTK+G+YDLNFKKQPNDGAHVHSAH LGELYKQFV DFPIVSI
Sbjct: 301 GYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSI 360

Query: 361 EDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTE 420
           EDPFDQDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTE
Sbjct: 361 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTE 420

Query: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR
Sbjct: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480

Query: 481 IEEELGNVRYAGESFRSP 492
           IEEELG+VRYAG SFRSP
Sbjct: 481 IEEELGSVRYAGASFRSP 496

BLAST of CmoCh11G002460 vs. ExPASy TrEMBL
Match: A0A6J1BZW1 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 456/498 (91.57%), Postives = 468/498 (93.98%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-------LRSHGVRCSVAVAP 60
           MASL SPP    NLL+ +LLPSK  S I SIPSSSSS         LRS  VRCSVAVAP
Sbjct: 1   MASLSSPP--AMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAP 60

Query: 61  SASTKASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120
           SA+TKAS E+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 61  SAATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDG 120

Query: 121 DKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180
           DK+VYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV
Sbjct: 121 DKSVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180

Query: 181 SLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240
           SLSVCRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 181 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240

Query: 241 AASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKA 300
           A SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKA
Sbjct: 241 ATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 300

Query: 301 GYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSI 360
           GYTGKIKIGMDVAASEFFTKDG+YDLNFKKQPNDGAHVHSAHGLGELYKQF+ DFPIVSI
Sbjct: 301 GYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSI 360

Query: 361 EDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTE 420
           EDPFDQDDWSSW+SLQSSV +Q+VGDDLLVTNPKRIAEAI KKAC ALLLKVNQIGSVTE
Sbjct: 361 EDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTE 420

Query: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR
Sbjct: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480

Query: 481 IEEELGNVRYAGESFRSP 492
           IEEELG+VRYAGESFRSP
Sbjct: 481 IEEELGSVRYAGESFRSP 496

BLAST of CmoCh11G002460 vs. NCBI nr
Match: XP_022931709.1 (enolase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 958.0 bits (2475), Expect = 3.2e-275
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
           SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG
Sbjct: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. NCBI nr
Match: KAG6587623.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 956.1 bits (2470), Expect = 1.2e-274
Identity = 490/491 (99.80%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
            EFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG
Sbjct: 61  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. NCBI nr
Match: KAG7021584.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 956.1 bits (2470), Expect = 1.2e-274
Identity = 490/491 (99.80%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS
Sbjct: 58  MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 117

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
            EFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG
Sbjct: 118 REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 177

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 178 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 237

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 238 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 297

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 298 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 357

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 358 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 417

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 418 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 477

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 478 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 537

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 538 VRYAGESFRSP 548

BLAST of CmoCh11G002460 vs. NCBI nr
Match: XP_022965656.1 (enolase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 949.5 bits (2453), Expect = 1.1e-272
Identity = 486/491 (98.98%), Postives = 487/491 (99.19%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVA APS STKAS
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAAAPSVSTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
            EFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 61  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL KYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLTKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. NCBI nr
Match: XP_023531804.1 (enolase 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.8 bits (2446), Expect = 7.3e-272
Identity = 485/491 (98.78%), Postives = 487/491 (99.19%), Query Frame = 0

Query: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKAS 60
           MASLPSPPTTTTNLLN+SLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVA APS STKAS
Sbjct: 1   MASLPSPPTTTTNLLNRSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAAAPSVSTKAS 60

Query: 61  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 120
            EFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 61  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240
           GAGAK LPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA
Sbjct: 181 GAGAKRLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360
           IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 480

Query: 481 VRYAGESFRSP 492
           VRYAGESFRSP
Sbjct: 481 VRYAGESFRSP 491

BLAST of CmoCh11G002460 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 783.5 bits (2022), Expect = 1.0e-226
Identity = 399/483 (82.61%), Postives = 432/483 (89.44%), Query Frame = 0

Query: 9   TTTTNLLNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKASSEFKLKSV 68
           TT  + L +S L     S    + S+ S    R  GV+CSV          + E ++K V
Sbjct: 4   TTKPHHLQRSFLSPSRVSGERYLESAPSCLRFRRSGVQCSV---------VAKECRVKGV 63

Query: 69  KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGGKGVLTAVK 128
           KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+K
Sbjct: 64  KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIK 123

Query: 129 NINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLP 188
           NIN+++APKL+GVDVRNQ DVDA+MLE+DGT NKSKLGANAILGVSLSVCRAGAGAKG+P
Sbjct: 124 NINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVP 183

Query: 189 LYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEALRMGSEVY 248
           LY+HIQE S TKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGA SF+EA +MGSEVY
Sbjct: 184 LYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVY 243

Query: 249 HTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIKIGMDVAAS 308
           HTLKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIKIGMDVAAS
Sbjct: 244 HTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAAS 303

Query: 309 EFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQDDWSSWASL 368
           EFF KDGRYDLNFKKQPNDGAHV SA  L +LY++F+ DFPIVSIEDPFDQDDWSSWASL
Sbjct: 304 EFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASL 363

Query: 369 QSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGV 428
           QSSV +Q+VGDDLLVTNPKRIAEAI+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGV
Sbjct: 364 QSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGV 423

Query: 429 MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGNVRYAGESF 488
           MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELGNVRYAGE+F
Sbjct: 424 MVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAF 477

Query: 489 RSP 492
           RSP
Sbjct: 484 RSP 477

BLAST of CmoCh11G002460 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 579.7 bits (1493), Expect = 2.2e-165
Identity = 301/435 (69.20%), Postives = 351/435 (80.69%), Query Frame = 0

Query: 68  VKARQIIDSRGNPTVEVDLITDDLYR--SAVPSGASTGIYEALELRDGDKNVYGGKGVLT 127
           VKARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG  + Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLGKGVSK 66

Query: 128 AVKNINDILAPKLVGVDVRNQEDVDAIML-EIDGTTN-----KSKLGANAILGVSLSVCR 187
           AV N+N+I+ P L+G D   Q  +D  M+ E+DGT N     K KLGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 188 AGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAE 247
           AGA   G+PLY+HI  L+   ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGAASF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 248 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKI 307
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 308 KIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQ 367
            IGMDVAASEF+++D  YDLNFK++ N+G+   S   L +LYK FV ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 368 DDWSSWASLQSSVG--VQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQA 427
           DDW  +A + +  G  VQIVGDDLLVTNPKR+A+AI +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 428 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 487
              SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 488 LGN-VRYAGESFRSP 492
           LG+   YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

BLAST of CmoCh11G002460 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 459.1 bits (1180), Expect = 4.3e-129
Identity = 246/478 (51.46%), Postives = 332/478 (69.46%), Query Frame = 0

Query: 15  LNKSLLPSKPQSPIISIPSSSSSKPLRSHGVRCSVAVAPSASTKASSEFKLKSVKARQII 74
           L+K +L  K +  + +   + +S P+         AV+ S  TK         VKARQI+
Sbjct: 7   LDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVS-SVITK---------VKARQIL 66

Query: 75  DSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKNVYGGKGVLTAVKNINDI 134
           DSRG PTVEVDL T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V  AVKNIN+ 
Sbjct: 67  DSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEK 126

Query: 135 LAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHI 194
           ++  L+G+D + Q  +D  M+++D T  KS+LGANAIL VS++ C+AGA  K +PL +H+
Sbjct: 127 ISEALIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHL 186

Query: 195 QELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEALRMGSEVYHTLKG 254
            +LS    +V+PVPAF V++GG HA N  A+QE MILP+GA+ F EAL+ GSE YH LK 
Sbjct: 187 SDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKA 246

Query: 255 IIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIKIGMDVAASEFFTK 314
           +I  K G   CNVG++GG AP++   +EGL L+ +AI + GY  KIKI +D+AA+  F  
Sbjct: 247 VISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATN-FCL 306

Query: 315 DGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQDDWSSWASLQSSVG 374
             +YDL+ K     G +  SA  + ++YK+  ND+PIVSIEDPFD++DW       SS+G
Sbjct: 307 GTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEH-TKYFSSLG 366

Query: 375 V-QIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSH 434
           + Q+VGDDLL++N KR+  AIQ+ +CNALLLKVNQIG+VTE+I+    ++ A WGV+ SH
Sbjct: 367 ICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSH 426

Query: 435 RSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN-VRYAGESFR 490
           R GETED+FI+DLSVGLA+G IK GAPCR ER  KYNQLLRIEEELG+   YAGE ++
Sbjct: 427 RCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9C41.4e-22582.61Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1[more]
P428947.1e-16970.28Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1[more]
P263002.7e-16870.09Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1[more]
P091044.6e-16868.91Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3[more]
Q54RK51.0e-16769.37Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EV051.5e-275100.00Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=... [more]
A0A6J1HM995.5e-27398.98Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111465484 PE=3 SV=1[more]
A0A5D3DYS65.3e-25291.97Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7US841.2e-25191.77Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A6J1BZW11.2e-25191.57Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022931709.13.2e-275100.00enolase 1, chloroplastic [Cucurbita moschata][more]
KAG6587623.11.2e-27499.80Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7021584.11.2e-27499.80Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022965656.11.1e-27298.98enolase 1, chloroplastic [Cucurbita maxima][more]
XP_023531804.17.3e-27298.78enolase 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G74030.11.0e-22682.61enolase 1 [more]
AT2G36530.12.2e-16569.20Enolase [more]
AT2G29560.14.3e-12951.46cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 166..182
score: 76.74
coord: 401..415
score: 80.2
coord: 223..236
score: 76.19
coord: 378..389
score: 78.16
coord: 96..110
score: 88.38
coord: 430..447
score: 83.0
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 66..487
e-value: 1.1E-165
score: 549.4
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 66..478
e-value: 0.0
score: 625.2
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 29..491
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 63..478
score: 43.486534
IPR000941EnolaseCDDcd03313enolasecoord: 67..477
e-value: 0.0
score: 739.681
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 201..491
e-value: 4.3E-193
score: 657.5
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 202..489
e-value: 4.8E-147
score: 488.9
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 65..193
e-value: 3.4E-84
score: 295.7
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 66..193
e-value: 8.8E-56
score: 187.7
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 59..186
e-value: 7.2E-56
score: 189.9
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 65..196
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 187..491
e-value: 9.1E-153
score: 509.7
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 201..486
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 66..478
e-value: 0.0
score: 625.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..41
NoneNo IPR availablePANTHERPTHR11902:SF42ENOLASE 1, CHLOROPLASTICcoord: 29..491
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 401..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G002460.1CmoCh11G002460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0010090 trichome morphogenesis
cellular_component GO:0009507 chloroplast
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity