CmoCh11G002440 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G002440
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	protein MIS12 homolog
Location	Cmo_Chr11: 1219749 .. 1221998 (-)
RNA-Seq Expression	CmoCh11G002440
Synteny	CmoCh11G002440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTCGGGAGCTTCCATTTGCTCTCGATTTAAATTTCTTCTCTAGTTTTTCATAAGGAAAGATGCATTTTTGAGGAAGGGAAAGACATTTTTTCATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCGTATGCTTCTCTCTTTCTCATCTCTTCAATTTGGATTTATAATTTTGTTCGGTTAAGTTCAGTCCTGTGAGTTCTTTTCCCCTTCAGACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGTAAGGTTTAGTCGTTAATTTCCGATTTCTTAAGTTGGTACTCTGAAGCACTACGAAATTGTTCCGGTTTTCTTTGAATTAAATTGTTTATTCTACCTAGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTGGGTTCTTCTACTCTCTCTGTGAAGAGGCTCCTGCTACTTTTATTTGGCGAAATTGCTCTATTATTGCTGTTTTTGTAACTGAATTCATTTCATAAGTGAATAATTGGGCATGTAAAAATGACTGTTTGCTTATGCAGCGAGTTTCCCACTTCTAATGTGTATATATGTACTGGCTTGGGAGCTTACTCAATTTTTGAAAAACTTGTATTGCTGTTGCATCATCAAGTTTAATTTAGATCTAAATCTTTTCTTTAAGTTGTCGTAGGATGAATCTCCTGCTGACACTTCAATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGCTATCGAGTGCTTCGTATGTTTATTACTTCTTGTGTTTCTGTATATCATATTTCATAAGAAAATATAATTTTCAGGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGGTAATTAAGTTATGCTTAGAATTGATTGTTTCTTCGTGCATTCCTGACCATACATGCGCCATTATCCTATTAGTATATTCATGAAGGGGAAGACTCGAAATGAAATATACAAATAATGTGTGGAGCTGATTTATTTTATTTTTCTTGGAATAAGTTTTTATTGTCATAGCCCGCTTGGTAGATTAAATTTTTTGCAGACGCTGACTTTCTCCTCTGATTGATCATCCTCGTCTGCATAACCACGTGATACAAACTCTAATACATCCAAATAATATTTTAGTGACTTTCTCCTCTGGTTTTGTTTATGGGACTTTATTTTGCATCTAGATTTTCTCCGACTTTATTTTGCATCTAGATTTTCACAACGATCATAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAATCTCTCCTTAGCAGATGCGTTTTAAAACCGTGAGGTTGATGACGATACGTAACAGACCAAAGCGGACAATATCTGTTAGCGGTAGACTTGGACCATTACATGAGATTTCTCTTTGTTTTCTTTGGGGTTCATCCGGGGAACTCTAGTCTGTGAATAATGCAATTTCTCTGGCTGTTTTCATTTTGAAAGCCACTTGAAACACAATTTATTCATTAGCATTAAATTTCATTGTACCTAAATTTAACAGTTGGCAGGTAAAAGATGTTCATGATTTTAGGAATCAGAATTCTAACAAATATCAAATTCTTGCAGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTATGATTTTCATTTTTCAAGCTACTTCAGCATCAATAAGCTTGTTTTAGCTTTGTGGAAGATGAATATCATCTAACATCCTATTTAATGCTTGTTTCTATTCTTCAGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGAACTTCTTCATTATGACATCAACCTTACTGTATATGATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCCATG

mRNA sequence

AGCTCGGGAGCTTCCATTTGCTCTCGATTTAAATTTCTTCTCTAGTTTTTCATAAGGAAAGATGCATTTTTGAGGAAGGGAAAGACATTTTTTCATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGATGAATCTCCTGCTGACACTTCAATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGCTATCGAGTGCTTCGTATGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGAACTTCTTCATTATGACATCAACCTTACTGTATATGATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCCATG

Coding sequence (CDS)

ATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGATGAATCTCCTGCTGACACTTCAATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGCTATCGAGTGCTTCGTATGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGA

Protein sequence

MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLDTELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

Homology

[Close]

BLAST of CmoCh11G002440 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 9.7e-50
Identity = 119/250 (47.60%), Postives = 165/250 (66.00%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDL 60
           MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVD 120
             GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP ++ES   +S+    LYD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSS 180
           LD ELDSLR+KL      + V K +VEL+ E QALER + S        NEAL+LY++SS
Sbjct: 121 LDAELDSLRDKL------NVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESS 180

Query: 181 VNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKL 240
           ++E+F+EM   AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL
Sbjct: 181 MDEIFKEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKL 235

Query: 241 DDIQEFLAYL 245
           +D+++F A L
Sbjct: 241 EDLEKFQAEL 235

[Close]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL
Match: A0A6J1EZI4 (protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 3.9e-126
Identity = 240/246 (97.56%), Postives = 240/246 (97.56%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELDSLRNKLSE      VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN
Sbjct: 121 TELDSLRNKLSE------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240

Query: 241 LAYLNL 247
           LAYLNL
Sbjct: 241 LAYLNL 240

[Close]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL
Match: A0A6J1F096 (protein MIS12 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437868 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.2e-103
Identity = 208/246 (84.55%), Postives = 208/246 (84.55%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN                    
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
                             VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN
Sbjct: 121 ------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 208

Query: 241 LAYLNL 247
           LAYLNL
Sbjct: 241 LAYLNL 208

[Close]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.5e-103
Identity = 198/244 (81.15%), Postives = 217/244 (88.93%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LRNKLSE      VRKEN+ LNQE QALER+ ASSNSQ N FNEALQLYE++SVN
Sbjct: 121 TELDFLRNKLSE------VRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGD SHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEF 238

Query: 241 LAYL 245
           LA L
Sbjct: 241 LADL 238

[Close]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.3e-102
Identity = 198/244 (81.15%), Postives = 216/244 (88.52%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LR  LSE      VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVN
Sbjct: 121 TELDFLRKNLSE------VRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEF 238

Query: 241 LAYL 245
           LA L
Sbjct: 241 LADL 238

[Close]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.3e-101
Identity = 198/245 (80.82%), Postives = 216/245 (88.16%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LR  LSE      VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVN
Sbjct: 121 TELDFLRKNLSE------VRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 180

Query: 181 EMFQ-EMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQE 240
           EMFQ EMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDIQE
Sbjct: 181 EMFQAEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQE 239

Query: 241 FLAYL 245
           FLA L
Sbjct: 241 FLADL 239

[Close]

BLAST of CmoCh11G002440 vs. NCBI nr
Match: XP_022931718.1 (protein MIS12 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 460.7 bits (1184), Expect = 8.0e-126
Identity = 240/246 (97.56%), Postives = 240/246 (97.56%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELDSLRNKLSE      VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN
Sbjct: 121 TELDSLRNKLSE------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240

Query: 241 LAYLNL 247
           LAYLNL
Sbjct: 241 LAYLNL 240

[Close]

BLAST of CmoCh11G002440 vs. NCBI nr
Match: XP_023530578.1 (protein MIS12 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.0 bits (1107), Expect = 6.8e-117
Identity = 225/246 (91.46%), Postives = 229/246 (93.09%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           ME SK +VVFDSLNLDP LFINEALNVVDDLVGDA DFYQSQAS ALK+EGSDRSQDLIM
Sbjct: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTL QSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELDSLRNKLSE      VRKENVELN EF  LERKTASSNSQANYFNEALQLYEQSSVN
Sbjct: 121 TELDSLRNKLSE------VRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMF+EMMGTASDLRA+IGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDI E 
Sbjct: 181 EMFKEMMGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEI 240

Query: 241 LAYLNL 247
           LAYLNL
Sbjct: 241 LAYLNL 240

[Close]

BLAST of CmoCh11G002440 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.3e-104
Identity = 202/244 (82.79%), Postives = 219/244 (89.75%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LRNKLSE      VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVN
Sbjct: 121 TELDFLRNKLSE------VRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEF 238

Query: 241 LAYL 245
           LA L
Sbjct: 241 LADL 238

[Close]

BLAST of CmoCh11G002440 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.9 bits (995), Expect = 6.6e-104
Identity = 201/244 (82.38%), Postives = 218/244 (89.34%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDL +
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LRNKLSE      VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVN
Sbjct: 121 TELDFLRNKLSE------VRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEF 238

Query: 241 LAYL 245
           LA L
Sbjct: 241 LADL 238

[Close]

BLAST of CmoCh11G002440 vs. NCBI nr
Match: KAG7023263.1 (Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-103
Identity = 200/244 (81.97%), Postives = 218/244 (89.34%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVN 180
           TELD LRNKLSE      VRKENV LNQE QALER+ ASSNSQ N FNEALQLYE++SVN
Sbjct: 121 TELDFLRNKLSE------VRKENVVLNQELQALERQAASSNSQVNRFNEALQLYERNSVN 180

Query: 181 EMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEF 240
           EMFQEMMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 181 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEF 238

Query: 241 LAYL 245
           LA L
Sbjct: 241 LADL 238

[Close]

BLAST of CmoCh11G002440 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 198.4 bits (503), Expect = 6.9e-51
Identity = 119/250 (47.60%), Postives = 165/250 (66.00%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDL 60
           MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVD 120
             GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP ++ES   +S+    LYD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNKLSELSSASYVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSS 180
           LD ELDSLR+KL      + V K +VEL+ E QALER + S        NEAL+LY++SS
Sbjct: 121 LDAELDSLRDKL------NVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESS 180

Query: 181 VNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKL 240
           ++E+F+EM   AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL
Sbjct: 181 MDEIFKEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKL 235

Query: 241 DDIQEFLAYL 245
           +D+++F A L
Sbjct: 241 EDLEKFQAEL 235

The following BLAST results are available for this feature:

BLAST of CmoCh11G002440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	9.7e-50	47.60	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of CmoCh11G002440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EZI4	3.9e-126	97.56	protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 P...	[more]
A0A6J1F096	1.2e-103	84.55	protein MIS12 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437868 P...	[more]
A0A6J1E0A1	3.5e-103	81.15	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	1.3e-102	81.15	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1JD43	3.3e-101	80.82	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of CmoCh11G002440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022931718.1	8.0e-126	97.56	protein MIS12 homolog isoform X1 [Cucurbita moschata]	[more]
XP_023530578.1	6.8e-117	91.46	protein MIS12 homolog [Cucurbita pepo subsp. pepo]	[more]
KAG6589575.1	1.3e-104	82.79	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515499.1	6.6e-104	82.38	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7023263.1	1.5e-103	81.97	Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmoCh11G002440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	6.9e-51	47.60	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 136..163
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 10..133 e-value: 2.5E-17 score: 63.3
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G002440.1	CmoCh11G002440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus