CmoCh11G001400 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G001400
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationCmo_Chr11: 705347 .. 706955 (-)
RNA-Seq ExpressionCmoCh11G001400
SyntenyCmoCh11G001400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTGCTACGGTTGCCAGCATTTGGATCCAAGCATTTGCGGTCCTTCCTACACCTTCGGCATTTACTCCTCCGCCCTCAAATCATCTCAAAACTACGATCAATCCACTCTCGACACTGTCTCCGTCTTCAAGGACATCGGCGCCACCGCCGGAGTTCTTGCCGGCTTACTCTACTCCGCTGTCGTCTCCACGGATCGCCCCCGTCGCCCATGGATCGTCCTCTCCGTCGGAGCAATTCAGTGCTTCCTTGGTTATATTTTCATTTGGGCTGCCGTCTCTGGTTTAATCCCTCGGCCGCCGGTGTCGGCTATGTGTTTGTTCATGTTCTTAGCCGTCCATGCCCAAGTCTTCTTCAACACGGCAAATGTCGTCACTGGCGTTCATAACTTCCAGCTCTACGGCGGCACCATCATCGGCATTCTAAAGGTCTGTTCTGCTCTGTCTGGAAATTCCCCAATTCGAATCCGAAAACTTAAGTCGTTTCTTTGAACAGGGATTTCTGGGTCTAAGTGGAGCAGTTCTGATCCAATTCTCTAACACATTCTACAGCGGAGACCCAATAAGTTATCTTCTGATGCTGGCGATTTTACCGGCGCTCACGACTGTTCTGCTCATGAGATTCGTCGTAATCGAAAAAACAGAGATCGGTAACGAATCGACCCATTTGAACTCTCTCTCAAAAATTGCTCTTATAATCTCTTGTTATCTCACAATTCTCATCATTTTGGACAACGTTTTCTTTTTACCAACATGGGTTCGCTTATTCACCTTCGTTCTACTCCTCATCCTTCTCGCTTCCCCTCTCGGAATCGCAAACAGAGCACAAACAGAGGACTCTGTTTCCAAAACCAAGCTTCAAAGCACAGACAATTCCGTTGAATATCATCAAATTCCCGGTGAAGAACAGAGAAATGATCAGCTAATGATGGTTACAAATGGAGAAATGAACGTAATCGAAGCAATTGGCACGGTGAATTTCTGGCTATTGTTTTCTGCGATGATGTGCGGAATGGGTTCTGGGTTAGCCACCATAAACAACATGAACCAACTGGGCCAGTCTTTGGGCTACAAAACAGTCGAAATCCACACATTCGTTTCACTGTGGAGCATTTGGAATTTCCTTGGGCGGTTGGGATCCGGCTATGCCTCTGATCTTCTCCTCCGGGAATTAGGCTGGGCGAGGCCATTGTTAATGGCCGTCGCCTTGCTGACGATGAGCGTCGGCCATATCATCATAGCTTCAGGCTTCTCCGGGAATTTGTACTTGGGTTCGGTGATTGTGGGGATTTGTTACGGCTCGCAATGGTCGCTAATGCCGGCAATTACGGCGGAGATTTTTGGAATAAGGCATATGGGTACCATTTACAATACGATCACAGTAGCCAGTCCGATTGGATCTTATGTTTTGTCTGTTAGAGTTATTGGGTATATTTACGACAGAGAAGCCTCTGCCGGAGACAACTCCTGCTCCGGCAAACACTGCTTTATGACGTCTTTTCTGATCATGGCAGCGGTGGCATTCTTGGGTTTTATTGTTGCTGTTGCTCTGTTCCTTCGGACCAGGAGATTCTATCAACTTCTTGTGCAGAGACAGCTCAAGGATTAG

mRNA sequence

ATGGATTGCTACGGTTGCCAGCATTTGGATCCAAGCATTTGCGGTCCTTCCTACACCTTCGGCATTTACTCCTCCGCCCTCAAATCATCTCAAAACTACGATCAATCCACTCTCGACACTGTCTCCGTCTTCAAGGACATCGGCGCCACCGCCGGAGTTCTTGCCGGCTTACTCTACTCCGCTGTCGTCTCCACGGATCGCCCCCGTCGCCCATGGATCGTCCTCTCCGTCGGAGCAATTCAGTGCTTCCTTGGTTATATTTTCATTTGGGCTGCCGTCTCTGGTTTAATCCCTCGGCCGCCGGTGTCGGCTATGTGTTTGTTCATGTTCTTAGCCGTCCATGCCCAAGTCTTCTTCAACACGGCAAATGTCGTCACTGGCGTTCATAACTTCCAGCTCTACGGCGGCACCATCATCGGCATTCTAAAGGGATTTCTGGGTCTAAGTGGAGCAGTTCTGATCCAATTCTCTAACACATTCTACAGCGGAGACCCAATAAGTTATCTTCTGATGCTGGCGATTTTACCGGCGCTCACGACTGTTCTGCTCATGAGATTCGTCGTAATCGAAAAAACAGAGATCGGTAACGAATCGACCCATTTGAACTCTCTCTCAAAAATTGCTCTTATAATCTCTTGTTATCTCACAATTCTCATCATTTTGGACAACGTTTTCTTTTTACCAACATGGGTTCGCTTATTCACCTTCGTTCTACTCCTCATCCTTCTCGCTTCCCCTCTCGGAATCGCAAACAGAGCACAAACAGAGGACTCTGTTTCCAAAACCAAGCTTCAAAGCACAGACAATTCCGTTGAATATCATCAAATTCCCGGTGAAGAACAGAGAAATGATCAGCTAATGATGGTTACAAATGGAGAAATGAACGTAATCGAAGCAATTGGCACGGTGAATTTCTGGCTATTGTTTTCTGCGATGATGTGCGGAATGGGTTCTGGGTTAGCCACCATAAACAACATGAACCAACTGGGCCAGTCTTTGGGCTACAAAACAGTCGAAATCCACACATTCGTTTCACTGTGGAGCATTTGGAATTTCCTTGGGCGGTTGGGATCCGGCTATGCCTCTGATCTTCTCCTCCGGGAATTAGGCTGGGCGAGGCCATTGTTAATGGCCGTCGCCTTGCTGACGATGAGCGTCGGCCATATCATCATAGCTTCAGGCTTCTCCGGGAATTTGTACTTGGGTTCGGTGATTGTGGGGATTTGTTACGGCTCGCAATGGTCGCTAATGCCGGCAATTACGGCGGAGATTTTTGGAATAAGGCATATGGGTACCATTTACAATACGATCACAGTAGCCAGTCCGATTGGATCTTATGTTTTGTCTGTTAGAGTTATTGGGTATATTTACGACAGAGAAGCCTCTGCCGGAGACAACTCCTGCTCCGGCAAACACTGCTTTATGACGTCTTTTCTGATCATGGCAGCGGTGGCATTCTTGGGTTTTATTGTTGCTGTTGCTCTGTTCCTTCGGACCAGGAGATTCTATCAACTTCTTGTGCAGAGACAGCTCAAGGATTAG

Coding sequence (CDS)

ATGGATTGCTACGGTTGCCAGCATTTGGATCCAAGCATTTGCGGTCCTTCCTACACCTTCGGCATTTACTCCTCCGCCCTCAAATCATCTCAAAACTACGATCAATCCACTCTCGACACTGTCTCCGTCTTCAAGGACATCGGCGCCACCGCCGGAGTTCTTGCCGGCTTACTCTACTCCGCTGTCGTCTCCACGGATCGCCCCCGTCGCCCATGGATCGTCCTCTCCGTCGGAGCAATTCAGTGCTTCCTTGGTTATATTTTCATTTGGGCTGCCGTCTCTGGTTTAATCCCTCGGCCGCCGGTGTCGGCTATGTGTTTGTTCATGTTCTTAGCCGTCCATGCCCAAGTCTTCTTCAACACGGCAAATGTCGTCACTGGCGTTCATAACTTCCAGCTCTACGGCGGCACCATCATCGGCATTCTAAAGGGATTTCTGGGTCTAAGTGGAGCAGTTCTGATCCAATTCTCTAACACATTCTACAGCGGAGACCCAATAAGTTATCTTCTGATGCTGGCGATTTTACCGGCGCTCACGACTGTTCTGCTCATGAGATTCGTCGTAATCGAAAAAACAGAGATCGGTAACGAATCGACCCATTTGAACTCTCTCTCAAAAATTGCTCTTATAATCTCTTGTTATCTCACAATTCTCATCATTTTGGACAACGTTTTCTTTTTACCAACATGGGTTCGCTTATTCACCTTCGTTCTACTCCTCATCCTTCTCGCTTCCCCTCTCGGAATCGCAAACAGAGCACAAACAGAGGACTCTGTTTCCAAAACCAAGCTTCAAAGCACAGACAATTCCGTTGAATATCATCAAATTCCCGGTGAAGAACAGAGAAATGATCAGCTAATGATGGTTACAAATGGAGAAATGAACGTAATCGAAGCAATTGGCACGGTGAATTTCTGGCTATTGTTTTCTGCGATGATGTGCGGAATGGGTTCTGGGTTAGCCACCATAAACAACATGAACCAACTGGGCCAGTCTTTGGGCTACAAAACAGTCGAAATCCACACATTCGTTTCACTGTGGAGCATTTGGAATTTCCTTGGGCGGTTGGGATCCGGCTATGCCTCTGATCTTCTCCTCCGGGAATTAGGCTGGGCGAGGCCATTGTTAATGGCCGTCGCCTTGCTGACGATGAGCGTCGGCCATATCATCATAGCTTCAGGCTTCTCCGGGAATTTGTACTTGGGTTCGGTGATTGTGGGGATTTGTTACGGCTCGCAATGGTCGCTAATGCCGGCAATTACGGCGGAGATTTTTGGAATAAGGCATATGGGTACCATTTACAATACGATCACAGTAGCCAGTCCGATTGGATCTTATGTTTTGTCTGTTAGAGTTATTGGGTATATTTACGACAGAGAAGCCTCTGCCGGAGACAACTCCTGCTCCGGCAAACACTGCTTTATGACGTCTTTTCTGATCATGGCAGCGGTGGCATTCTTGGGTTTTATTGTTGCTGTTGCTCTGTTCCTTCGGACCAGGAGATTCTATCAACTTCTTGTGCAGAGACAGCTCAAGGATTAG

Protein sequence

MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD
Homology
BLAST of CmoCh11G001400 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 7.5e-20
Identity = 125/526 (23.76%), Postives = 228/526 (43.35%), Query Frame = 0

Query: 15  GPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPWIV 74
           G ++ F  YSS LKS     Q  L+ ++V  D+G   G  +G+       +       +V
Sbjct: 58  GTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLS-------VV 117

Query: 75  LSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQLY 134
           L   A   F+GY   W  ++ +I   P S + L   LA  +  +FNTA  +  + +F   
Sbjct: 118 LFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSICWFNTACFILCIRHFPNN 177

Query: 135 GGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVV----IE 194
               + +   F G+S A+     N         YLL+ +++P + +   +  V+    ++
Sbjct: 178 RALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLD 237

Query: 195 KTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLIL-LASPLGI 254
            T   +   H + +  I  +++   +  ++L +       +     V+LL+  L +PL +
Sbjct: 238 TTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLVFPLCAPLLV 297

Query: 255 ANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLM---------MVTNGEMNVIE-- 314
             R   +  +     +    S  Y  +  +E +N +           M T  E N +   
Sbjct: 298 YAR---DYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEGNTVRLG 357

Query: 315 -------AIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWN 374
                   I  + FWL + A  CG   GL   NN+ Q+ QSLG  +    T V+++S ++
Sbjct: 358 DEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNST---TLVTIYSSFS 417

Query: 375 FLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGN--LYLGSVIVGIC 434
           F GRL S  A D + +     R    A+ALL   +   ++A   S    L   + ++G+ 
Sbjct: 418 FFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLS 477

Query: 435 YGSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREAS------- 494
            G  ++   +IT+++FG   +G  +N +    PIGS +L   +   IY+  AS       
Sbjct: 478 SGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIYEANASPDITPIV 537

Query: 495 AGDNSCSGKHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQ 509
           +    C G+ C+  +F+    ++ LG + +++L++RT+  Y  L Q
Sbjct: 538 SDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRLEQ 567

BLAST of CmoCh11G001400 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.3e-11
Identity = 55/187 (29.41%), Postives = 96/187 (51.34%), Query Frame = 0

Query: 327 NQLGQSLGYKTVE------IHTFVSLWSIWNFLGRLGSGYASDLLLRELGWARPLLMAVA 386
           N +G  L   TVE        T VSL++ ++ + RL  G++S+ +  E   +RP+L++V 
Sbjct: 293 NNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVI 352

Query: 387 LLTMSVGHIIIASG----FSGNLYLG--SVIVGICYGSQWSLMPAITAEIFGIRHMGTIY 446
            L  +  H+++ SG    F    Y    +++ G  YGS ++L+P I  +++GI ++GTI+
Sbjct: 353 ALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW 412

Query: 447 NTITVASPIGSYVLSVRVIGYIYDREASAGDNS----CSGKHCFMTSFLIMAAVAFLGFI 498
            +  +A  +GS    + +   +YD  +  G  S    CSG HC+  +F+I      L F 
Sbjct: 413 GSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITG--TGLAFA 472

BLAST of CmoCh11G001400 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.6e-08
Identity = 71/296 (23.99%), Postives = 128/296 (43.24%), Query Frame = 0

Query: 221 LDNVFFLPTWVRLFTFVLLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEE 280
           L  VF    W+     V++ I  +    I + A   +   +   + +DN VE H    E+
Sbjct: 199 LGRVFKAFAWIYTVIGVMIWIATSKVAQIQHEADIMEEQDR-MAEPSDNDVENHYDDNEQ 258

Query: 281 QRNDQLMMVTNG-EMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSL--GYKT 340
            R   L+  T+  +M +++       ++  + + C +G     I NM  L   L  G++ 
Sbjct: 259 SR---LLHATHAQQMTLMKVFRDPVLYIFGATIFCALGPLEMFIANMGSLTNVLAGGHEP 318

Query: 341 VEIHTFVSLWSIWNFLGRLGSGYASDLLLR---ELGWARPLLMAVALLTMSVGHIIIASG 400
                 +S++++ + L RLG+G   D   +    + W   L + V L+T    +++  S 
Sbjct: 319 AMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVGLVTQGKIYMLSMSS 378

Query: 401 --------FSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSY 460
                    +  L+   ++ GI YG  +++ P IT  ++G +  GT Y T+ +A  +GS 
Sbjct: 379 LDHSHMVTINRKLFYIGIMQGIAYGGLFTIYPTITLMVWGEKMFGTAYGTLMIAPALGS- 438

Query: 461 VLSVRVIGYIYDRE-ASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVAVALFLRTRR 502
            LS  +   +YD E A++   SC       T+    AA+      V V +  R RR
Sbjct: 439 ALSCLIYADVYDSECANSTTRSCIAPVYETTALEFCAAIL---LTVVVTVLWRKRR 486

BLAST of CmoCh11G001400 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 4.0e-05
Identity = 39/149 (26.17%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 298 EAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLG 357
           EA G   FWLL+ A  CG  +GL  I ++   G+  G   +     VS  +  N   R+ 
Sbjct: 223 EAKGDTKFWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAAGAVSSLAFSNAATRIL 282

Query: 358 SGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLM 417
           SG+  D    ++G         AL T ++  I    G    L + ++++G  YG+ ++L 
Sbjct: 283 SGWFVD----KIGIRVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLF 342

Query: 418 PAITAEIFGIRHMGTIYNTITVASPIGSY 447
           PA   + +G    G+ Y  +  A  +  +
Sbjct: 343 PATCLQFYGPTAQGSNYGLLFTACGLAGF 367

BLAST of CmoCh11G001400 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 7.5e-04
Identity = 56/233 (24.03%), Postives = 96/233 (41.20%), Query Frame = 0

Query: 279 EEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTV 338
           EE+   +   + N E  +  A  T+  W L        G G A +NN+  + Q+L   T 
Sbjct: 354 EEEERRKKNWLLNYETRIFLADHTM--WWLALGFFLVTGPGEAYLNNLGTIVQTLNLDTT 413

Query: 339 EI---------HTFVSLWSIWNFLGRLGSGYASDLLL----------------RELGWAR 398
            I          T V++ ++ + + RL +G  SDL                        R
Sbjct: 414 AIVDSHPAGLPSTHVTIIALTSTIARLLTGSLSDLFAPTARRHFTVDQETAGPDPFTKQR 473

Query: 399 PLLMAVAL-----LTMSVGHIIIASGFSGN----LYLGSVIVGICYGSQWSLMPAITAEI 458
           P L  +A      L +S+G +++AS    +     +L + +VG+ YGS +SL+P I + +
Sbjct: 474 PALSRLAFLIPSALLLSLGFLLLASPLPTHHPELSHLTTALVGLGYGSIFSLVPIIISVV 533

Query: 459 FGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAG---DNSCSGKHCF 475
           +G+ + GT +  + +    G+ +  V +    Y   A      D  C G  CF
Sbjct: 534 WGVENFGTNWGIVAMFPAAGAAMWGV-IYSRAYQSAADGSPTDDGQCHGWKCF 583

BLAST of CmoCh11G001400 vs. ExPASy TrEMBL
Match: A0A6J1EZJ6 (uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC111440723 PE=4 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 2.9e-285
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0

Query: 1   MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS 60
           MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS
Sbjct: 1   MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS 60

Query: 61  AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN 120
           AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN
Sbjct: 61  AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN 120

Query: 121 TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT 180
           TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT
Sbjct: 121 TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT 180

Query: 181 VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL 240
           VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL
Sbjct: 181 VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL 240

Query: 241 ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI 300
           ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI
Sbjct: 241 ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI 300

Query: 301 GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY 360
           GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY
Sbjct: 301 GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY 360

Query: 361 ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI 420
           ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI
Sbjct: 361 ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI 420

Query: 421 TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI 480
           TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI
Sbjct: 421 TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI 480

Query: 481 MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 514
           MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD
Sbjct: 481 MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 513

BLAST of CmoCh11G001400 vs. ExPASy TrEMBL
Match: A0A6J1BYJ5 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia OX=3673 GN=LOC111006908 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.0e-221
Identity = 411/503 (81.71%), Postives = 446/503 (88.67%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72
           ICGPSYTFGIYSSALKS+Q YDQSTLDTVSVF D+GATAGVLA LLYSAV S +RPRR W
Sbjct: 22  ICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATAGVLAVLLYSAVASNNRPRRSW 81

Query: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132
           I+ +VGAIQCF GYIFIWAAVSGLIPRPPV AMC FMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 82  IIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFLAVHAQVFFNTANVVTGVHNFQ 141

Query: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192
           LY  TI+GILKGF GL GAVLIQFSNTF + DP + L++L+ILPAL+T+LLM FVVI+KT
Sbjct: 142 LYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVLLSILPALSTLLLMAFVVIDKT 201

Query: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252
           E GNES HLNS S IALIIS YLTILIIL+N   LP W R+FTFVLLL L+ASPLGIA R
Sbjct: 202 ETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLIASPLGIAIR 261

Query: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312
           A+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTVNFWLL  AM
Sbjct: 262 ARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFWLLSFAM 321

Query: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372
           MCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASDLLLR+LG A
Sbjct: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLLRQLGLA 381

Query: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432
           RP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAEIFGIR+MGT
Sbjct: 382 RPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGT 441

Query: 433 IYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCSGKHCFMTSFLIMAAVAFLGFI 492
           I+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCSGKHCFMTSFL+MAAVAFLGF+
Sbjct: 442 IFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAVAFLGFL 501

Query: 493 VAVALFLRTRRFYQLLVQRQLKD 514
           VAV LF RTRRFYQ LVQR+LKD
Sbjct: 502 VAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of CmoCh11G001400 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.7e-184
Identity = 344/522 (65.90%), Postives = 411/522 (78.74%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRR-- 72
           ICG SYTF IYSSALKS+Q+YDQSTLDTVSVFKDIGA AG+++G LYSAV   + PRR  
Sbjct: 23  ICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISGFLYSAVTPLN-PRRAF 82

Query: 73  --PWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGV 132
             PW+V + GAIQ FLGYIFIWAAVSG+I RPPV AMC FMFLA HAQ FFNTANVVTGV
Sbjct: 83  AGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGV 142

Query: 133 HNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVV 192
           HNF  Y GTI+GI+KG+LGLSGA+LIQ  NT  + DP ++LLMLA+LP + +++ M FV 
Sbjct: 143 HNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSNFLLMLAVLPTVLSIMFMWFVR 202

Query: 193 IEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLG 252
           I+KTE  NE  HLNSLS +A+I++ YL ++IIL+N F L +W R FTF +LLILLA+PLG
Sbjct: 203 IDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLG 262

Query: 253 IANRAQTED--------------SVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGE- 312
           IA  AQ ED               V+K +    ++SVEYH++P EE   DQ+M+V+N   
Sbjct: 263 IAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPREE---DQIMVVSNSRA 322

Query: 313 ---MNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIW 372
              MNV+EAI T+NFWLLF AM+CGMGSGLATINNM+QLGQSLGY   E  TFVSLWSIW
Sbjct: 323 PQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIW 382

Query: 373 NFLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICY 432
           NFLGR G+GY SD L    GWARPLLMA+ LL MS GHI+IASGFSGNLY+GS++VGICY
Sbjct: 383 NFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICY 442

Query: 433 GSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSG 492
           GSQWSLMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ SVRVIGYIYDREA+    +CSG
Sbjct: 443 GSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGACSG 502

Query: 493 KHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQRQLK 513
            HCF+ SFL+MA VAFLGF+VA ALF RTRRFY+L +QR+++
Sbjct: 503 THCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of CmoCh11G001400 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.7e-184
Identity = 344/522 (65.90%), Postives = 411/522 (78.74%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRR-- 72
           ICG SYTF IYSSALKS+Q+YDQSTLDTVSVFKDIGA AG+++G LYSAV   + PRR  
Sbjct: 23  ICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISGFLYSAVTPLN-PRRAF 82

Query: 73  --PWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGV 132
             PW+V + GAIQ FLGYIFIWAAVSG+I RPPV AMC FMFLA HAQ FFNTANVVTGV
Sbjct: 83  AGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGV 142

Query: 133 HNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVV 192
           HNF  Y GTI+GI+KG+LGLSGA+LIQ  NT  + DP ++LLMLA+LP + +++ M FV 
Sbjct: 143 HNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSNFLLMLAVLPTVLSIMFMWFVR 202

Query: 193 IEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLG 252
           I+KTE  NE  HLNSLS +A+I++ YL ++IIL+N F L +W R FTF +LLILLA+PLG
Sbjct: 203 IDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLG 262

Query: 253 IANRAQTED--------------SVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGE- 312
           IA  AQ ED               V+K +    ++SVEYH++P EE   DQ+M+V+N   
Sbjct: 263 IAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPREE---DQIMVVSNSRA 322

Query: 313 ---MNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIW 372
              MNV+EAI T+NFWLLF AM+CGMGSGLATINNM+QLGQSLGY   E  TFVSLWSIW
Sbjct: 323 PQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIW 382

Query: 373 NFLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICY 432
           NFLGR G+GY SD L    GWARPLLMA+ LL MS GHI+IASGFSGNLY+GS++VGICY
Sbjct: 383 NFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICY 442

Query: 433 GSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSG 492
           GSQWSLMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ SVRVIGYIYDREA+    +CSG
Sbjct: 443 GSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGACSG 502

Query: 493 KHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQRQLK 513
            HCF+ SFL+MA VAFLGF+VA ALF RTRRFY+L +QR+++
Sbjct: 503 THCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of CmoCh11G001400 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 7.2e-183
Identity = 344/521 (66.03%), Postives = 408/521 (78.31%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRR-- 72
           ICG SYTF IYSSALKS+Q+YDQSTLDTVSVFKDIGA AG+++G LYSAV   + PRR  
Sbjct: 23  ICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISGFLYSAVTPFN-PRRAF 82

Query: 73  --PWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGV 132
             PW+V + GAIQ FLGYIFIWAAVSG+I RPPV AMC FMFLA HAQ FFNTANVVTGV
Sbjct: 83  AGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGV 142

Query: 133 HNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVV 192
           HNF  Y GTI+GI+KG+LGLSGA+LIQ  NT  + DP ++LLMLA+LP + +V+ M FV 
Sbjct: 143 HNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSNFLLMLAVLPTVLSVMFMWFVR 202

Query: 193 IEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLG 252
           I+KTE  NE  HLNSLS +A+I++ YL ++IIL+N F L +W R FTF +LLILLA+PLG
Sbjct: 203 IDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLG 262

Query: 253 IANRAQTED--------------SVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGE- 312
           IA  AQ ED               V+K +    ++SVEYH++P EE   +Q+M+V+N   
Sbjct: 263 IAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEYHELPREE---NQIMVVSNTRA 322

Query: 313 ---MNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIW 372
              MNV+EAI T+NFWLLF AM+CGMGSGLATINNM+QLGQSLGY   E  TFVSLWSIW
Sbjct: 323 PQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTETETKTFVSLWSIW 382

Query: 373 NFLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICY 432
           NFLGR G+GY SD L    GWARPLLMA+ LL MS GHI+IASGFSGNLY+GS++VGICY
Sbjct: 383 NFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICY 442

Query: 433 GSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSG 492
           GSQWSLMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ SVRVIGYIYDREA+    +CSG
Sbjct: 443 GSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAREHGACSG 502

Query: 493 KHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQRQL 512
            HCF+ SF +MA VAFLGF+VA ALF RTRRFYQL  QR++
Sbjct: 503 IHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRV 539

BLAST of CmoCh11G001400 vs. NCBI nr
Match: XP_022933362.1 (uncharacterized protein LOC111440723 [Cucurbita moschata])

HSP 1 Score: 990.3 bits (2559), Expect = 6.0e-285
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0

Query: 1   MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS 60
           MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS
Sbjct: 1   MDCYGCQHLDPSICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYS 60

Query: 61  AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN 120
           AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN
Sbjct: 61  AVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFN 120

Query: 121 TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT 180
           TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT
Sbjct: 121 TANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTT 180

Query: 181 VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL 240
           VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL
Sbjct: 181 VLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLL 240

Query: 241 ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI 300
           ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI
Sbjct: 241 ILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAI 300

Query: 301 GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY 360
           GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY
Sbjct: 301 GTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGY 360

Query: 361 ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI 420
           ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI
Sbjct: 361 ASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAI 420

Query: 421 TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI 480
           TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI
Sbjct: 421 TAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLI 480

Query: 481 MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 514
           MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD
Sbjct: 481 MAAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 513

BLAST of CmoCh11G001400 vs. NCBI nr
Match: KAG6587515.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 949.1 bits (2452), Expect = 1.5e-272
Identity = 495/501 (98.80%), Postives = 496/501 (99.00%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72
           ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRP RPW
Sbjct: 21  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPHRPW 80

Query: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132
           IVLSVGAIQCFLGYIFIWAAVSGLIPRP VS MCLFMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 81  IVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFLAVHAQVFFNTANVVTGVHNFQ 140

Query: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192
           LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT
Sbjct: 141 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 200

Query: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252
           EIGNESTHLNSLSKIALIISCYLTILIILDNVF LPTWVRLFTFVLLLILLASPLGIANR
Sbjct: 201 EIGNESTHLNSLSKIALIISCYLTILIILDNVFILPTWVRLFTFVLLLILLASPLGIANR 260

Query: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312
           AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM
Sbjct: 261 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 320

Query: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372
           MCGMGSGLATINNMNQLGQSLGYK VEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA
Sbjct: 321 MCGMGSGLATINNMNQLGQSLGYKPVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 380

Query: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432
           RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT
Sbjct: 381 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 440

Query: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGF+VA
Sbjct: 441 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 500

Query: 493 VALFLRTRRFYQLLVQRQLKD 514
           VALFLRTRRFYQLLVQRQLKD
Sbjct: 501 VALFLRTRRFYQLLVQRQLKD 521

BLAST of CmoCh11G001400 vs. NCBI nr
Match: XP_023530984.1 (uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 940.3 bits (2429), Expect = 7.2e-270
Identity = 489/501 (97.60%), Postives = 495/501 (98.80%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72
           ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGA+AGVLAGLLYSAVVSTDRPRRPW
Sbjct: 21  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGASAGVLAGLLYSAVVSTDRPRRPW 80

Query: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132
           IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 81  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 140

Query: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192
           LYGGTIIGILKGFLGLSGAVLIQFSNTFY+GDPI+YLLMLAILPALTTVLLMRFVVI+KT
Sbjct: 141 LYGGTIIGILKGFLGLSGAVLIQFSNTFYNGDPINYLLMLAILPALTTVLLMRFVVIQKT 200

Query: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252
           EIGNESTHLNSLSKIALIISCYLTILIILDNV  LPTWVRLFTFV+LLILLASPLGIANR
Sbjct: 201 EIGNESTHLNSLSKIALIISCYLTILIILDNVLILPTWVRLFTFVILLILLASPLGIANR 260

Query: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312
           AQTEDS SKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM
Sbjct: 261 AQTEDSASKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 320

Query: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372
           MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA
Sbjct: 321 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 380

Query: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432
           RPLLMAVALLTMSVGHIIIASG S NLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT
Sbjct: 381 RPLLMAVALLTMSVGHIIIASGSSWNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 440

Query: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGF+VA
Sbjct: 441 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 500

Query: 493 VALFLRTRRFYQLLVQRQLKD 514
            ALFLRTRRFYQLLVQRQLKD
Sbjct: 501 FALFLRTRRFYQLLVQRQLKD 521

BLAST of CmoCh11G001400 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937.6 bits (2422), Expect = 4.7e-269
Identity = 490/500 (98.00%), Postives = 493/500 (98.60%), Query Frame = 0

Query: 13   ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72
            ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRP RPW
Sbjct: 561  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPHRPW 620

Query: 73   IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132
            IVLSVGAIQCFLGYIFIWAAVSGLIPRP VS MCLFMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 621  IVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFLAVHAQVFFNTANVVTGVHNFQ 680

Query: 133  LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192
            LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT
Sbjct: 681  LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 740

Query: 193  EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252
            EIGNESTHLNSLSKIALIISCYLTILIILDNVF L TWVRLFTFVLLLILLASPLGIANR
Sbjct: 741  EIGNESTHLNSLSKIALIISCYLTILIILDNVFILTTWVRLFTFVLLLILLASPLGIANR 800

Query: 253  AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312
            AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM
Sbjct: 801  AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 860

Query: 313  MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372
            MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA
Sbjct: 861  MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 920

Query: 373  RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432
            RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT
Sbjct: 921  RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 980

Query: 433  IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492
            IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGF+VA
Sbjct: 981  IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 1040

Query: 493  VALFLRTRRFYQLLVQRQLK 513
            VALFLRTRRFYQLL +R+ K
Sbjct: 1041 VALFLRTRRFYQLLGKRKRK 1060

BLAST of CmoCh11G001400 vs. NCBI nr
Match: XP_022134701.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia])

HSP 1 Score: 779.2 bits (2011), Expect = 2.1e-221
Identity = 411/503 (81.71%), Postives = 446/503 (88.67%), Query Frame = 0

Query: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72
           ICGPSYTFGIYSSALKS+Q YDQSTLDTVSVF D+GATAGVLA LLYSAV S +RPRR W
Sbjct: 22  ICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATAGVLAVLLYSAVASNNRPRRSW 81

Query: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132
           I+ +VGAIQCF GYIFIWAAVSGLIPRPPV AMC FMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 82  IIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFLAVHAQVFFNTANVVTGVHNFQ 141

Query: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192
           LY  TI+GILKGF GL GAVLIQFSNTF + DP + L++L+ILPAL+T+LLM FVVI+KT
Sbjct: 142 LYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVLLSILPALSTLLLMAFVVIDKT 201

Query: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252
           E GNES HLNS S IALIIS YLTILIIL+N   LP W R+FTFVLLL L+ASPLGIA R
Sbjct: 202 ETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLIASPLGIAIR 261

Query: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312
           A+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTVNFWLL  AM
Sbjct: 262 ARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFWLLSFAM 321

Query: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372
           MCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASDLLLR+LG A
Sbjct: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLLRQLGLA 381

Query: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432
           RP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAEIFGIR+MGT
Sbjct: 382 RPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGT 441

Query: 433 IYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCSGKHCFMTSFLIMAAVAFLGFI 492
           I+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCSGKHCFMTSFL+MAAVAFLGF+
Sbjct: 442 IFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAVAFLGFL 501

Query: 493 VAVALFLRTRRFYQLLVQRQLKD 514
           VAV LF RTRRFYQ LVQR+LKD
Sbjct: 502 VAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of CmoCh11G001400 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 565.8 bits (1457), Expect = 3.4e-161
Identity = 301/511 (58.90%), Postives = 378/511 (73.97%), Query Frame = 0

Query: 15  GPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRR---- 74
           G SYTFGIYS+ LKS+Q+YDQSTLDTVSVFKDIGA AGV +GLLY+   S     R    
Sbjct: 22  GASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLYTYATSNRLRGRGGGI 81

Query: 75  -----PWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVV 134
                PW+VL+VGAIQCF GY  IWA+V+GLI +PPV  MCLFMFLA  +Q FFNTANVV
Sbjct: 82  GGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTANVV 141

Query: 135 TGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMR 194
           + V NF  YGGT +GI+KGFLGLSGA+LIQ   T  +GDP S++L+LA+ P + ++L+M 
Sbjct: 142 SAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFILLLAVTPTVLSLLVMP 201

Query: 195 FVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLAS 254
            V I +T + ++  HLN LS ++LII+ YL I+IIL N F L +W  + T V LL++LA 
Sbjct: 202 LVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSWANIVTLVCLLVMLAL 261

Query: 255 PLGIANRAQTEDSVSKT------KLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEA 314
           PL IA RAQ  D + KT       L S+  +        E     +  +  N  +N+++A
Sbjct: 262 PLLIARRAQ-RDGMEKTVPHDYSPLISSPKATTSGNQSSEGDSKVEAGLSEN--LNLLQA 321

Query: 315 IGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSG 374
           +  ++FWLLF AM+CGMGSGL+TINN+ Q+G+SL Y +VEI++ VSLWSIWNFLGR G+G
Sbjct: 322 MKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAG 381

Query: 375 YASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPA 434
           YASD LL + GW RPLLMA  L TMS+GH+IIASGF GNLY+GSVIVG+CYGSQWSLMP 
Sbjct: 382 YASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPT 441

Query: 435 ITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFL 494
           IT+E+FGIRHMGTI+NTI+VASPIGSY+ SVR+IGYIYD+ AS   N+C G HCF  SF+
Sbjct: 442 ITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTASGEGNTCYGSHCFRLSFI 501

Query: 495 IMAAVAFLGFIVAVALFLRTRRFY-QLLVQR 510
           IMA+VAF GF+VA+ LF RT+  Y Q+LV+R
Sbjct: 502 IMASVAFFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CmoCh11G001400 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 538.9 bits (1387), Expect = 4.5e-153
Identity = 285/506 (56.32%), Postives = 367/506 (72.53%), Query Frame = 0

Query: 12  SICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPR-- 71
           S  G SYTFGIYSS LKSSQ+YDQSTLDTVSV+KDIGA  G+L+GL Y+AV S       
Sbjct: 19  SFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGLFYTAVASRKSGNGG 78

Query: 72  ---RPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVT 131
               PW+V+ VG +Q F+GY FIW A SG+IPRPPV+ MCLFMF A H Q FFNTA VVT
Sbjct: 79  FFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFAGHCQPFFNTAIVVT 138

Query: 132 GVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRF 191
            V NF  YGGT +GI+KG+LGLSGA+L+Q  + F  GDP +Y+L+LA++P+L  + LM F
Sbjct: 139 AVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLLAVVPSLLILTLMPF 198

Query: 192 VVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASP 251
           V    T I  +  HLN LS I+LII  YL ++I+++N+  +   +++ +F  LL+LLASP
Sbjct: 199 VRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMKICSFTFLLLLLASP 258

Query: 252 LGIANRAQTEDSVSKTKLQ-STDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNF 311
           L +A RAQ E+      L             P     +D   ++TN +MNV+EAI T NF
Sbjct: 259 LLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKDVMTN-DMNVLEAICTTNF 318

Query: 312 WLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLL 371
           WLLF AM+CGMGSGLATINN+ Q+G+SL Y TV++++ VSLWSIWNFLGR GSGY SD  
Sbjct: 319 WLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSIWNFLGRFGSGYISDTY 378

Query: 372 LRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIF 431
           L   GW RP+ MA+ L  M++GHI++ASG  G+LY+GS++VG+ YGSQWSLMP IT+EIF
Sbjct: 379 LHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLAYGSQWSLMPTITSEIF 438

Query: 432 GIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVA 491
           G+ HMGTI+ TI++ASP+GSY  SV+VIGY+YD+ AS  D+SC G HCF TSFLIMAA+A
Sbjct: 439 GVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDHSCYGNHCFRTSFLIMAAMA 498

Query: 492 FLGFIVAVALFLRTRRFYQLLVQRQL 512
            LG +VA+ L LRT++FY  LV +++
Sbjct: 499 LLGSLVALVLLLRTKKFYATLVAKRI 523

BLAST of CmoCh11G001400 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 270/504 (53.57%), Postives = 362/504 (71.83%), Query Frame = 0

Query: 15  GPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPR----- 74
           G SYTFGIYS+ LKS+Q+YDQSTLDTVSVFKDIG   GVL+GL+Y+A     R R     
Sbjct: 24  GGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLSGLVYTAATFNRRRRDGRER 83

Query: 75  --RPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTG 134
              PW+V+ +GAI  F GY  +WA+V+GLI RPPV  MCLFMF+A  +  F NTANVV+ 
Sbjct: 84  RGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLFMFIAAQSLTFLNTANVVSS 143

Query: 135 VHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFV 194
           + NF  YGGT +GI+KGF+GLSGA+LIQ       GDP +++L+LAI+P+L +VL+M  V
Sbjct: 144 LENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKTFILLLAIVPSLLSVLVMPLV 203

Query: 195 VIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPL 254
            + KT   +E  HL+ LS ++LII+ YL I IIL +   LP+W    T  +LL+LL+SPL
Sbjct: 204 RVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSLPSWANAVTLAVLLVLLSSPL 263

Query: 255 GIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWL 314
            +A RA   DS+ K         V+  +        + LM+  +  +N+++A+  V+FWL
Sbjct: 264 LVAVRAH-RDSIEKPLSSVYSPLVDNLE---ATTSGEILMLDEDKSLNLLQAMCNVDFWL 323

Query: 315 LFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLR 374
           LF AM+CGMGSG++TINN+ Q+G+SL Y +VEI++ ++LW+IWNF+GR G GY SD LL 
Sbjct: 324 LFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLH 383

Query: 375 ELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGI 434
             GW RPLLMA  L TM++GH+IIASGF GNLY GS+IVGICYGSQWSLMP IT+E+FG+
Sbjct: 384 RKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGV 443

Query: 435 RHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFL 494
           +HMGTIYNTI++ASP+GSY+ SVR+IGYIYDR      N+C G HCF  +++++A+VAFL
Sbjct: 444 KHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTIIGEGNTCYGPHCFRLAYVVIASVAFL 503

Query: 495 GFIVAVALFLRTRRFYQLLVQRQL 512
           GF+V+  L  RT+  Y+ + ++ L
Sbjct: 504 GFLVSCVLVFRTKTIYRQIFEKIL 523

BLAST of CmoCh11G001400 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.5e-145
Identity = 278/509 (54.62%), Postives = 363/509 (71.32%), Query Frame = 0

Query: 12  SICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRP-RR 71
           S  G +YTF IYSS LKSSQ+YDQSTLD VSVFKDIG T G+++G LY+A+ S  R    
Sbjct: 19  SFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLYTAMTSKSRGFGG 78

Query: 72  PWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHN 131
           PW+V+ VG +Q F+G+ FIWA+V GLI  PPV  MCLF+FLA H+  FFNTANVVT   N
Sbjct: 79  PWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPFFNTANVVTAARN 138

Query: 132 FQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGD--PISYLLMLAILPALTTVLLMRFVV 191
           F  YGGT +GI++GFLGLSGA+LIQ  +    G+  P +++L+LAI+P L   L M FV 
Sbjct: 139 FSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVPTLVMFLAMPFVR 198

Query: 192 IEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLG 251
           + +T   ++  HL+ LS I++II+ YL ++I ++NV  L   +++F+F+L+L+LLASPL 
Sbjct: 199 VYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFSFILVLLLLASPLL 258

Query: 252 IANRAQTEDSVSKTKLQSTDNSV----EYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVN 311
           +A RA  E    +  L S D  V         P      D   +V   + N++EA+ TVN
Sbjct: 259 VAVRALRE---KRQTLSSLDGPVLDTSALLDPPSSNIFPDGDHLVAE-DSNILEAMSTVN 318

Query: 312 FWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDL 371
           FWLLF AM+CGMGSG AT+NNM Q+G+SL Y +V++++ VSLWSIWNFLGR G+GY SD 
Sbjct: 319 FWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNFLGRFGAGYVSDT 378

Query: 372 LLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEI 431
            L +  W RP+ MA+ L  M++GHII+ASG  G+LY GSV++G+ YGSQWSLMP IT+EI
Sbjct: 379 FLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGSQWSLMPTITSEI 438

Query: 432 FGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAV 491
           FGIRHMGTIY TI++A PIGSY+LSV+VIGY YD+ AS  DNSC G  CF TSF+IMA+V
Sbjct: 439 FGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASEDDNSCFGSQCFRTSFMIMASV 498

Query: 492 AFLGFIVAVALFLRTRRFYQ-LLVQRQLK 513
           A  G +VA  LF RT +FY+ L+ +R LK
Sbjct: 499 ALFGSLVASVLFFRTHKFYKNLVAKRNLK 523

BLAST of CmoCh11G001400 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 4.0e-85
Identity = 198/544 (36.40%), Postives = 305/544 (56.07%), Query Frame = 0

Query: 12  SICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRP 71
           S  G +Y FGIYS  +K +  YDQ+TL+ +S FKD+GA  GVLAGLL       +    P
Sbjct: 33  STAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLL-------NEVTPP 92

Query: 72  WIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNF 131
           W +L +GAI  F GY  IW AV+  I +P V  MCL++ +  ++Q F NT ++VT V NF
Sbjct: 93  WFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNF 152

Query: 132 QLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEK 191
               G ++GILKG++GLSGA++ Q    FY  D    +LM+  LPA+ +   +R + I K
Sbjct: 153 PESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRIMK 212

Query: 192 TE-IGNESTHLNSLSKIALIISCYLTILIILDNV--FFLPTWVRLFTFVLLLILL----- 251
            +   NE     +   I+L ++ +L ++II++ +  F    +      V++L+LL     
Sbjct: 213 VKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVV 272

Query: 252 ---------ASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVT---- 311
                       + + + A       K KL S+    E+    GEE +     + T    
Sbjct: 273 ILEEKKLWKEKQVALNDPAPINVVTEKPKLDSS----EFKDDDGEESKEVVEKVKTPSCW 332

Query: 312 ---------NGEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIH 371
                      +  +++A+ +V+  +LF A +CG+G  L  I+N+ Q+G SLGY    + 
Sbjct: 333 TTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVS 392

Query: 372 TFVSLWSIWNFLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYL 431
           TFVSL SIWN+ GR+ SG  S++ L +  + RPL++ + LL    GH++IA    G LY+
Sbjct: 393 TFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYV 452

Query: 432 GSVIVGICYGSQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA 491
            SVI+G C+G+QW L+ AI +EIFG+++  T+YN  +VASPIGSY+L+VRV GY+YD EA
Sbjct: 453 ASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEA 512

Query: 492 ------------SAGDNSCSGKHCFMTSFLIMAAVAFLGFIVAVALFLRTRRFYQLLVQR 514
                          D +C G  CF  SF+I+AAV   G +V++ L +RT++FY+  + +
Sbjct: 513 GKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYK 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E17.5e-2023.76Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU88.3e-1129.41Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Q6FWD46.6e-0823.99Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ... [more]
A0LNN54.0e-0526.17L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) ... [more]
Q5AXV17.5e-0424.03Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / ... [more]
Match NameE-valueIdentityDescription
A0A6J1EZJ62.9e-285100.00uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC1114407... [more]
A0A6J1BYJ51.0e-22181.71LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia... [more]
A0A5A7UT751.7e-18465.90Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BWC21.7e-18465.90protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ... [more]
A0A0A0LUE87.2e-18366.03Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847... [more]
Match NameE-valueIdentityDescription
XP_022933362.16.0e-285100.00uncharacterized protein LOC111440723 [Cucurbita moschata][more]
KAG6587515.11.5e-27298.80Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023530984.17.2e-27097.60uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo][more]
KAG7021495.14.7e-26998.00Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022134701.12.1e-22181.71LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G74780.13.4e-16158.90Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.14.5e-15356.32Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.11.1e-14653.57Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.11.5e-14554.62Major facilitator superfamily protein [more]
AT2G39210.14.0e-8536.40Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 12..249
e-value: 7.3E-55
score: 186.2
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 307..500
e-value: 6.8E-10
score: 38.4
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 296..504
e-value: 4.4E-9
score: 38.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 230..501
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 13..512
NoneNo IPR availablePANTHERPTHR21576:SF22F25A4.25 PROTEINcoord: 13..512
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 12..488
e-value: 3.29628E-103
score: 312.649
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 303..513
score: 9.676043

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G001400.1CmoCh11G001400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity