CmoCh10G006820 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh10G006820
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	RNA methyltransferase
Location	Cmo_Chr10: 3113394 .. 3117486 (-)
RNA-Seq Expression	CmoCh10G006820
Synteny	CmoCh10G006820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGCTGCCCATGGGTTTGCCGCGAAAATTAGGAAGCGCATAACAGTGCTGGGGGAAGCTGGTTTAAACCCTAGAGATATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTATTCACCTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGGTATTTCTCCTGACCTATTCTTCCCCACTTACCTCGGAGTTCTAGGTTGCCCTTCTTGGGTCTTCACGTGGTAAATATAGTTCTGAGAAGTATTCTCGATTGTCTCCGGTTTCTGGTTAAGATTCGAACTATTATCGGTTAGTGATAGAAACGAGAATTTAAAATTTCGAGAATTGGTGTAGACCAGTCAAGCTTGTTTTTCCAACTTCAGTGCTCTAATAATTTATGATTCGTTCTTATTCGGCCGACAGAATGTGCGTTACCCCTAGTTGCGTTTTCTTATTTTTTGTGTGGCAGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGGTAGCAATACTTCAATCTCAGTATTTATTTATTTATATATATATATATATATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAAAAAAAAAAAAAAAAAAAGGATTGATACGTCCTTATCATGTGCCGTAATTTTATGAACTTCTTACTCATTTTACCAAAGGATATGCATGGAAGTCTCAAGTCTGAAAAGAAGTTTATATGCATGTTGCAGCAAAGACAATAAAACTATAGCAACTTCTTGTCCTTTCGCTATGAACTTAAAACTGTCTTTTGTGCATCTCAGTTCAAGAAAGCTCTGTTATCACTGACTATTTTATTGTTCATTGAAGATTGTGGAAGTGCTTTTTCTCAAATACTAGAGCTCTTTACTTATGCGGATAACTTTCTTTTCGCAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGGTAAGATTTACTCTAAAAGCTCTACAACTTCCATTGCAGATCCACTTTATACTTCTACAAAGTACATGAAAGCGGTTTTGTGAATAACTATCTTCTTTGGAGGTCTAAATCTTAATTGATGATGCATTCTATCATTAACTATTTTAAGCACTTGTAAATTAGTCATGTAAATTATAGCCTGGGATGGTTGGATGCTGATTTATTGTTCGACTATATTATTAGGGAGATTAGTTGGAGAATTCTTATACAAAAAAAGTAATTGAAGGGTTTTACAGAAAAGGGTAGATATTAAAGGAGGTTGTTGATAAGGGTACGTGTGATAAATATTTAGATCTAAATAGTCTTGAGTGGTATGCCCTTTCCCGGTCCCCTATGGGCTGTAATTATAACCTGTAACACACACATATTTTTTTGGCTTTGTCCTATCGGATTGCCTCTTAGTAACTTACTTCATGGGTGGGAACTCGAATCTCTGTTCCTCCTTTATATTGATAGAATCAAAGCTCGTGACCTGAAATTATTGAGGGAACTTACATTGTTTGTTGAACTACATGGATCATAGTGACTTCATCAATACGAACTCTATCTTTTACTGGTGGCTTTAGTAATAATCACATCATGTTCTGGTAGTTATGTTAGGAGAAAATATATCTCAAAAGTGAAAGAATTGTTTGTATACCAAGGTACTTAGCCAGAGTTGATCTCCCAGTCTTTCTATACAAATGATTGCTGAGTTTCCAACCTGAACTTCTCTTTATGCAAGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGATAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGGTAAGTTAATCTTTTTATCTTGTGTTGCGTATTAATGCTATTTGTTTTCTTGACCCTTGATACATCTCCCTTCTGGGGTCACAATATGCGTTTTTTGTTTTTTTTGTTTTTTTTCTGTACTGCAGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGTACTTGCCTTGTCATACACCTATGCACTTTGTGTTAAATGGTCCTAAGATCAATAATAACCGTCCTAAGATCAATAATAACCTTGGTCAGTTATATGTTTGTATTTACTTTAATGTTCGATTAAGAACCTTCTTAATTATGCATTCATTCTGAAAAGGCAGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGGTAAAAAATTTCATGCATCCAAATTGTTAATCAATAATTACTTTTAGTCAAACTTGAATTTTTTTTTGGGTGCTCTTCTAGCATCTCATATCATATTGCCTTAGATCGCTGATCGTTCTAATGCAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGGTATTTTTATGATTTATTTGGCTTAGTTTTACAAACAGCATCAGTTTTCACTAACTTCTGGACAAAATACCACCCCAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTAATCTGGACATCTGAACCAAATTGAATGCAGAGAGCCATCAGCTGGTCTTTAGAAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATATTCCAGACATGGTAAAGAGTGTTTGTTTTTGAAGGGTGCCGTCTGTAGTGGGATCCCTGTCACTTATGCGTAGGTGCTTTTTGAAGGCTTGTTGTAAGCTTCTGCTTTTTGATATTTACATCGTTATCCTTTTGAACAATCTGAACGCTGATATATATTACTATTCATGTACCGTTCA

mRNA sequence

GCGCTGCCCATGGGTTTGCCGCGAAAATTAGGAAGCGCATAACAGTGCTGGGGGAAGCTGGTTTAAACCCTAGAGATATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTATTCACCTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGATAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTAATCTGGACATCTGAACCAAATTGAATGCAGAGAGCCATCAGCTGGTCTTTAGAAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATATTCCAGACATGGTAAAGAGTGTTTGTTTTTGAAGGGTGCCGTCTGTAGTGGGATCCCTGTCACTTATGCGTAGGTGCTTTTTGAAGGCTTGTTGTAAGCTTCTGCTTTTTGATATTTACATCGTTATCCTTTTGAACAATCTGAACGCTGATATATATTACTATTCATGTACCGTTCA

Coding sequence (CDS)

ATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGATAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGA

Protein sequence

MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASGASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK

Homology

[Close]

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot
Match: Q6NPC9 (Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g51130 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.0e-80
Identity = 152/279 (54.48%), Postives = 198/279 (70.97%), Query Frame = 0


Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR-ARINVGKPDNGLELGASGA 122
           IGCNSG++TI IA+KF CR ILGVDID  R++DA+ +LR+  R+      +  +  + GA
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 SKI--DTEASTSIS-------SSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAK 182
             +    E S S+S       S++  DL   VSFQ++NF+ ++   +  YDTILCLSV K
Sbjct: 161 DGVHGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVTK 220

Query: 183 WIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPE 242
           W+HLNWGDDGLITLF KIWRLL PGGI V+EPQPWKSYE N  VSETT  N+++I + P+
Sbjct: 221 WVHLNWGDDGLITLFSKIWRLLQPGGIFVMEPQPWKSYENNRRVSETTAMNYRTIVLRPD 280

Query: 243 EFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
            FQEILLDKIGFRTVE LT+ LS ++  GF+R I  FQK
Sbjct: 281 RFQEILLDKIGFRTVEDLTSSLSGASK-GFDRQILAFQK 318

[Close]

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot
Match: A3KQ55 (7SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 4.2e-46
Identity = 106/293 (36.18%), Postives = 152/293 (51.88%), Query Frame = 0


Query: 14  QNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQ 73
           QNP++ KK  F YGNY  YYGYR      EDPR++V   +WF GKD LD+GCN+G +T+ 
Sbjct: 352 QNPQQPKK-KFQYGNYNKYYGYR-NPGMSEDPRIRVMNPDWFRGKDVLDLGCNTGHLTLF 411

Query: 74  IARKFNCRRILGVDIDPDRVDDAYRNLRRARINV-----------GKPDNGLELGASGAS 133
           IA+ +    I+G+DID   +  A +N+R     V            K D G E+      
Sbjct: 412 IAKNWRPASIVGLDIDGSLIHAARQNIRHYLSEVQVQHSRRSGENTKADRG-EVSGEEKD 471

Query: 134 KIDTE--------------------ASTSISSSKEHDLHTDVSFQQQNFIFSQ----YPP 193
           K  T                       T+  S    D   +V+F + N++          
Sbjct: 472 KDKTSKHSFPVSLRISRGPIAGPPLPETNTHSLPPGDFPANVTFIKGNYVLESDVLLQTQ 531

Query: 194 EKHYDTILCLSVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSE 253
            + YD ILCLSV KW+HLNWGD GL   F+++++ L PGG+ +LEPQPW SY     ++E
Sbjct: 532 REEYDVILCLSVTKWVHLNWGDAGLKRFFHRVYKHLRPGGLFILEPQPWSSYNKRKKLTE 591

Query: 254 TTKKNFKSIRILPEEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
              KN+ SIR+ P++F   L  ++GF + E + T  ++S   GF RPI ++ K
Sbjct: 592 AICKNYHSIRLKPDQFSSFLTTEVGFSSYELIGTSQNYSK--GFQRPISLYHK 639

[Close]

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot
Match: Q8K3A9 (7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.0e-44
Identity = 104/284 (36.62%), Postives = 156/284 (54.93%), Query Frame = 0


Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF+G+D LD+GCN G +T+ IA K
Sbjct: 384 KKQQLKFQYGNYCKYYGYR--NPSCEDVRLRVLKPEWFQGRDVLDLGCNVGHLTLSIACK 443

Query: 78  FNCRRILGVDIDPDRVDDAYRNLR-------RARINVGKPDNGLE--------------- 137
           +   R++G+DIDP  +  A +N+R       R +    + D G E               
Sbjct: 444 WGPARMVGLDIDPRLIHSARQNIRHYLSEELRLQAQTSEGDPGTEGEEGTITVRKRSCFP 503

Query: 138 ------LGASGASKIDTE-ASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCL 197
                  G   A ++  + A TS+  +    +  +    +   + +Q P    YD +LC 
Sbjct: 504 ASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDELVDAQRP---EYDVVLCF 563

Query: 198 SVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIR 257
           S+ KW+HLNWGD+GL  +F +I+R L PGGILVLEPQPW SY    S++ET  KN+  I+
Sbjct: 564 SLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYCKRKSLTETIYKNYFRIQ 623

Query: 258 ILPEEFQEILLD-KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           + PE+F   L   ++GF + E + T   ++ + GF RP+++F K
Sbjct: 624 LKPEQFSSYLTSPEVGFSSYELVAT--PNNTSRGFQRPVYLFHK 660

[Close]

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot
Match: Q7L2J0 (7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 5.1e-44
Identity = 104/285 (36.49%), Postives = 153/285 (53.68%), Query Frame = 0


Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF G+D LD+GCN G +T+ IA K
Sbjct: 407 KKQQRKFQYGNYCKYYGYR--NPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACK 466

Query: 78  FNCRRILGVDIDPDRVDDAYRNLR-------RARINVGKPDNGLELGASGASKIDTE--- 137
           +   R++G+DID   +  A +N+R       R      + D G E G  G + +      
Sbjct: 467 WGPSRMVGLDIDSRLIHSARQNIRHYLSEELRLPPQTLEGDPGAE-GEEGTTTVRKRSCF 526

Query: 138 --------------------ASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILC 197
                               A TS+  +    +  +    + + + +Q P    YD +LC
Sbjct: 527 PASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDDLVEAQTP---EYDVVLC 586

Query: 198 LSVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSI 257
           LS+ KW+HLNWGD+GL  +F +I+R L PGGILVLEPQPW SY    +++ET  KN+  I
Sbjct: 587 LSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLTETIYKNYYRI 646

Query: 258 RILPEEFQEILLD-KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           ++ PE+F   L    +GF + E + T   H+ + GF RP+++F K
Sbjct: 647 QLKPEQFSSYLTSPDVGFSSYELVAT--PHNTSKGFQRPVYLFHK 683

[Close]

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot
Match: Q9U2R0 (Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y17G7B.18 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.5e-43
Identity = 102/278 (36.69%), Postives = 156/278 (56.12%), Query Frame = 0


Query: 4   EQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDI 63
           ++EK+ AE ++         + YGN+  YYG RL      D RL VF+K+WFE K  LDI
Sbjct: 97  KEEKSAAENRKQKTEYFNKKYRYGNFDRYYGIRLNPG-ESDKRLSVFQKDWFEHKQALDI 156

Query: 64  GCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLR-----RARINVGKPDN-GLELG 123
           GCN+G +T+ IA+ F+ RRI+G+DID   +  A +N+R        ++   P + G++ G
Sbjct: 157 GCNAGFLTLSIAKDFSPRRIIGIDIDEHLIGVARKNIRHYCDHETEVSGKFPASFGVQFG 216

Query: 124 ASGASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPP----EKHYDTILCLSVAKW 183
                    EA  S S+        ++ F+++N++          +  +D IL LS+ KW
Sbjct: 217 TVSQR---NEAPRSFSTK----FPDNIWFKKENYVLESDEMLDMIQPEFDVILALSITKW 276

Query: 184 IHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEE 243
           IHLNWGDDG+   F + +  LHPGG L++EPQ + SY+    +SE  K N+  I   PE+
Sbjct: 277 IHLNWGDDGMRRFFRRAYAQLHPGGRLIIEPQAFDSYKKRAKMSEELKANYSKIEFKPED 336

Query: 244 FQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           F+  L++ +GF +VE L  G+  + + GF RPI V+ K
Sbjct: 337 FEMWLIETVGFESVEKL--GVVGAKSKGFERPIDVYLK 364

[Close]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL
Match: A0A6J1HBE1 (RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.5e-155
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271

[Close]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL
Match: A0A6J1JBF8 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 3.7e-154
Identity = 268/271 (98.89%), Postives = 270/271 (99.63%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELKQNPKRRKKHAFPYGNY+SYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKQNPKRRKKHAFPYGNYRSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           KIGFRTVEALTTGLSHSNTGGFNRPIFVF+K
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFRK 271

[Close]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL
Match: A0A5A7V9R8 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G001350 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.9e-124
Identity = 219/272 (80.51%), Postives = 244/272 (89.71%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR-ARINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ID EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

[Close]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL
Match: A0A5D3BG34 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold369G00080 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.9e-124
Identity = 219/272 (80.51%), Postives = 244/272 (89.71%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR-ARINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ID EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

[Close]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL
Match: A0A1S3CJ98 (RNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103501041 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.9e-124
Identity = 219/272 (80.51%), Postives = 244/272 (89.71%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR-ARINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ID EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

[Close]

BLAST of CmoCh10G006820 vs. TAIR 10
Match: AT5G51130.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.4e-81
Identity = 152/279 (54.48%), Postives = 198/279 (70.97%), Query Frame = 0


Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR-ARINVGKPDNGLELGASGA 122
           IGCNSG++TI IA+KF CR ILGVDID  R++DA+ +LR+  R+      +  +  + GA
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 SKI--DTEASTSIS-------SSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAK 182
             +    E S S+S       S++  DL   VSFQ++NF+ ++   +  YDTILCLSV K
Sbjct: 161 DGVHGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVTK 220

Query: 183 WIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPE 242
           W+HLNWGDDGLITLF KIWRLL PGGI V+EPQPWKSYE N  VSETT  N+++I + P+
Sbjct: 221 WVHLNWGDDGLITLFSKIWRLLQPGGIFVMEPQPWKSYENNRRVSETTAMNYRTIVLRPD 280

Query: 243 EFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
            FQEILLDKIGFRTVE LT+ LS ++  GF+R I  FQK
Sbjct: 281 RFQEILLDKIGFRTVEDLTSSLSGASK-GFDRQILAFQK 318

The following BLAST results are available for this feature:

BLAST of CmoCh10G006820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPC9	2.0e-80	54.48	Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
A3KQ55	4.2e-46	36.18	7SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV...	[more]
Q8K3A9	1.0e-44	36.62	7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 ...	[more]
Q7L2J0	5.1e-44	36.49	7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 S...	[more]
Q9U2R0	1.5e-43	36.69	Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y1...	[more]

BLAST of CmoCh10G006820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBE1	1.5e-155	100.00	RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1	[more]
A0A6J1JBF8	3.7e-154	98.89	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]
A0A5A7V9R8	8.9e-124	80.51	RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79...	[more]
A0A5D3BG34	8.9e-124	80.51	RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold36...	[more]
A0A1S3CJ98	8.9e-124	80.51	RNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103501041 PE=3 SV=1	[more]

BLAST of CmoCh10G006820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51130.1	1.4e-81	54.48	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041698	Methyltransferase domain 25	PFAM	PF13649	Methyltransf_25	coord: 61..107 e-value: 3.4E-6 score: 27.7
IPR010675	RNA methyltransferase bin3, C-terminal	PFAM	PF06859	Bin3	coord: 162..271 e-value: 8.7E-40 score: 135.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 22..271 e-value: 1.9E-74 score: 252.0
None	No IPR available	PANTHER	PTHR12315:SF0	7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME	coord: 4..271
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..203 e-value: 1.36007E-6 score: 43.9579
IPR039772	RNA methyltransferase Bin3-like	PANTHER	PTHR12315	BICOID-INTERACTING PROTEIN RELATED	coord: 4..271
IPR024160	Bin3-type S-adenosyl-L-methionine binding domain	PROSITE	PS51515	BIN3_SAM	coord: 44..271 score: 74.581657
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 25..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh10G006820.1	CmoCh10G006820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001510	RNA methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity