CmoCh10G001360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh10G001360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionE3 ubiquitin-protein ligase SIS3-like
LocationCmo_Chr10: 625356 .. 629571 (-)
RNA-Seq ExpressionCmoCh10G001360
SyntenyCmoCh10G001360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCATATTGCTCATTTCTCTTCTTCTGCCTCCAAAATTTCGCTCCTGTTGTCCTACCCATGATCCTACGAGAACCAGCTCTTGTTCTCGTGATTATTTCCGATTCATTGCCATCAGATTCCATCAGGCTTCTGCACCGTCGTATGGCGGTTTGTGATTCTCCATCCAGCTTGTTCTAGTGAAAATTTCTCTCATCTATGGCTGCTCGAGGGGTCGATTTCAAGTGGTGAGTCTTTCAGGACTTGCTCTTCAATTCAATGAAATTCTGTTTTAGCATAATCTATAGTATGGTGCCGGAGCAGTACTTCGTAGTTAGCTTGATAGGCTTTTGCGACTTATTTCTTCTTTTTGCAGGTATGACGGTTTTTTCTTGTCGATGCTCGCGATTAGTGTGTATCCTTCAGTGTTTTGGGGAATTTTAGAGTTTGTTTGTATCGGATTCTTTTCAGTCTTTTACAATGGTTGAATGATGACTCATGACTGCTGCATTTACTTTTATGGTTTGCTTTTTCACTTATTGTTTAGCCATGATTTAAAGCTTGAGTGGATTTGCTCTTTATATTTCTTTGGTATTAGAGTTTGAAACGAGACATACTCATATCCTATGCTTTAAAATCAAGAGCAATGTCTTTCTCTTAACGTTTTTTATTTGTGTATTTTCTTTAACTATTTTGCTCGATAGAGTAGTTGTTTCGATCAACTGGAAGAGGTACCATACTTGTGTACATCCCTTGCATATATGGATTTTGGTAAGTTGTACTCAACTTTCAACAAGTATCAGATATTATTATTAATTTGTTGTTCTCTTGTAATTTTTTGGAATTCAACGATAGTTTGTTTGTTTATTTTTTTTATTGTTATTTGTTGTACAGGTTGATTATACTACCGTCTTTGTTTTCCGTCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGTAAGGCTTCCAACTTATAGTTTTACGTCAATTTGGGCTAGCAAAATCTTACAGTTCTTCTATTAATTCTCCATTTGTGAAAAGCTTTAAAGAGATATTGTGTTGTCAGAAAGTAGATTAAAGGAGAAAAATGTTTCTCCAATACCTTCATTATGGATGGTATGGTTTGATCTTAACGGTTTCAATTATTGGGGCAAAGATCAAAACAAACATCGATTCTGACGTGCAAAGATTGTGGACTTTTGACTTCTCATCAGAAGCATGGTGACTGAAGTGTGAAAAAGAAAAGAAAAGAACATCTGTTGCTGATTTAAAGAATTATGTTCTGTTTGCCAAATTTGTTTTATTCTCTTGGGGCTAATTATGAAGCTTCAAGTCATGAATGTTCACTTTCTGGTTTTGTGTTGAATTACTATAAACCTTATTTCATTTAGAAATGGGGATGCTGCGTAAGTTTTTCTAGTCAAGTCAATTACATTTGACCAAGAGGTCTAGAACTGTTTATACCCATGAATTAATTATTTAGTTATATACATCCACACCTGCCTCTCCTCCCCTATTTTAATTGACAATACATTTTTCTCGCTCTATTTTTCTTTTGCAATATTTCTGTTTTGGTAGATGCCATTGGAGATTTTCTTGTCTTCTAAACTTGTGTATCGTAGGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTCTGTGGAAGAATTGTGGTCCTTTCAATCCTTGCACTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATCGGTACTTTATGGTTTAGAGAAGCCCATAAATGTGTATGTTATTTTCTATTTCAACTCCCATCGCTACCATTTGAATTTTCATACTTTTAAAAAATATTTATGGAGGTATTGGTTTAAATCTGTCAGCTTTTTGAGCAAGGCCAGAAATGGGGATTCCTTATTTGGCTTCTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCGTGTCATTGGGAAAGGTTGGTTCTATTATGTTTACTATGCAGATTATCATTGGAGTTCATTATGAATTACGTCTAGACTCTCCAATAATTTGTAGCTTGGCTAACAGTTGTTTTTATGTGCCACTTACACTTTTTAGCGAGTTCATGTTGAAAATTCTCAAATATTTTTCTTCATTTATACATGAAATTCACGTATTTATCTTTGAAACCTCCATGTAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTAAGCAATTAAGCTTCCTGCCTTCTTAGATTTTGGAACTTAAAAGGATGTGCATTAATTTTCTTTCTACGTTATCAATTTTTCTGCCTGATATTTGCAATGACGTCCATAGATCACCTATATTATATGCATATCTACTTTTATAAATTTTTTCTACAATATATTCTTTGCTTTCAATTAAAAAAAAAAAAAAAGCCTTTTTTTTTTTTGCCTGGTGATTCTTACAAAATGCAAACACCCGATGAATTGGTCATGAAGGAAGTTGAAGTGGACATTTTGAACTGGATTACGATTCAATCAAAATCATAAATATATCCTTTAGGTTCTTTTTAGTTTGTGGTTCTATTCTTAAAAAAATATATATTTGAAAGTTTGATTTTCTTATATTTTTTTAACAAGAAATTAAACTTCTCATTGATGAAATGAAAAGAGACCAATGCATAAAAGATATGAACTCCACAGGTGAATGAAATTGAAAACAGTAAACGGAATAAATAAATAAAGAGAATCACAATGGAAAGATAAAGACATTCCGGCTAAGACATAAAACTTGTAAACAGAAATCAACAAAAGACTTGAGAAAGAGTACATGAAAAGCATGACCTTTAGGGATAAAATACTTCTCGGGCATCAAATAAACATTTGTATCTTTCATTTTTTTCCCTCTATAATTTTGCCCATGTGACAAAACTGATAAAATACTTCTCGGGCATCAAATAAACATTTGTATCTTTCATTTTTTTCCCTCTATAATTTTGCCCATGTGACAAAACTGATAGTGAACAGTCTTTTATTACAGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCCGCAGGACAAGAAACAAGAGTAATGGGCCAAGATGCTGCTGCATATCATCCTGGACTTTATTTGACTCCTGCTCAGGTAAGTCTATTATCCCTGAGTTGACATTTTGTCATCAATATTCGTGAGGTTTTCCCAACACCTGTTGTCTGGTAGCATAAGAGTAGTTTTGTGCTGTTGCTACCATTTTCCCTCTTAGTGGCTATAGCTCATTAAAAATTCGAGGGAAGTTGATTGACATGAAATGAGAGCATTGTGAATTCGGAAGTTCTTCCGAAAGAGGGCTTGGTTTCTTAGGTTCTCAGCCTTTAAATTTTTCATGAGAGGTCTTGTCTAATTCTTTAAGAAATCTCTACTATGTAAACCATTATGTGGGTCCGTATTATGACCAAATTTTTTGTTTTTAATCCTGATGGTCAAGCTATTCGTTGATGCTTGGACAGAGGGAAGCTGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAACGAGGTAATTGATTTTTCTCGTCTTACCATTTCATTTATAGAAGTTCAGAAAACCAGTGGGTGTACAAGAATGTCGCTTGTTTATGATTTGTATTTTACTGCATTTCATGCTCTTTCTGGGAACCTAGATTCATGGTTATAATAAATACATGAAAGCACGAACCTTTTGTTGGTAGATTACTGAAAATGAAGTTACGGTCATTATAAATTGGTGCAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGATGCATTGACGAGTGGCTTCGACTAAATGTGAAATGCCCTAGATGTCGCTGCTCAGTCTTCCCAAACCTCGATCTTAGTGCTCTGTCCAATCTTCATTCTGATACTGAAAGGCCATCTGCAAGTGTTGTTACGGCCAACCGTTATATGAGACCTCAACCTTCCAGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGGCCTGTTCGCACAGAAAACACTGTTGCACACTGTGATTTAGAGTCTGCTAGTGCTTTGGAAGTTGCTGAGAATGCTGAGAATGGATCTTCCCATGACCAAACTATACCGGAAAGGTCTCCCGTAAGACCCATGCCTTTGAGTCAGTCCACGTCACAACGCTAG

mRNA sequence

GCTCATATTGCTCATTTCTCTTCTTCTGCCTCCAAAATTTCGCTCCTGTTGTCCTACCCATGATCCTACGAGAACCAGCTCTTGTTCTCGTGATTATTTCCGATTCATTGCCATCAGATTCCATCAGGCTTCTGCACCGTCGTATGGCGGTTTGTGATTCTCCATCCAGCTTGTTCTAGTGAAAATTTCTCTCATCTATGGCTGCTCGAGGGGTCGATTTCAAGTGGTATGACGGTTTTTTCTTGTCGATGCTCGCGATTAGTGTAGTAGTTGTTTCGATCAACTGGAAGAGGTACCATACTTGTGTACATCCCTTGCATATATGGATTTTGGTTGATTATACTACCGTCTTTGTTTTCCGTCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTCTGTGGAAGAATTGTGGTCCTTTCAATCCTTGCACTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATCGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTTTTGAGCAAGGCCAGAAATGGGGATTCCTTATTTGGCTTCTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCGTGTCATTGGGAAAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCCGCAGGACAAGAAACAAGAGTAATGGGCCAAGATGCTGCTGCATATCATCCTGGACTTTATTTGACTCCTGCTCAGAGGGAAGCTGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAACGAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGATGCATTGACGAGTGGCTTCGACTAAATGTGAAATGCCCTAGATGTCGCTGCTCAGTCTTCCCAAACCTCGATCTTAGTGCTCTGTCCAATCTTCATTCTGATACTGAAAGGCCATCTGCAAGTGTTGTTACGGCCAACCGTTATATGAGACCTCAACCTTCCAGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGGCCTGTTCGCACAGAAAACACTGTTGCACACTGTGATTTAGAGTCTGCTAGTGCTTTGGAAGTTGCTGAGAATGCTGAGAATGGATCTTCCCATGACCAAACTATACCGGAAAGGTCTCCCGTAAGACCCATGCCTTTGAGTCAGTCCACGTCACAACGCTAG

Coding sequence (CDS)

ATGGCTGCTCGAGGGGTCGATTTCAAGTGGTATGACGGTTTTTTCTTGTCGATGCTCGCGATTAGTGTAGTAGTTGTTTCGATCAACTGGAAGAGGTACCATACTTGTGTACATCCCTTGCATATATGGATTTTGGTTGATTATACTACCGTCTTTGTTTTCCGTCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTCTGTGGAAGAATTGTGGTCCTTTCAATCCTTGCACTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATCGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTTTTGAGCAAGGCCAGAAATGGGGATTCCTTATTTGGCTTCTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCGTGTCATTGGGAAAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCCGCAGGACAAGAAACAAGAGTAATGGGCCAAGATGCTGCTGCATATCATCCTGGACTTTATTTGACTCCTGCTCAGAGGGAAGCTGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAACGAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGATGCATTGACGAGTGGCTTCGACTAAATGTGAAATGCCCTAGATGTCGCTGCTCAGTCTTCCCAAACCTCGATCTTAGTGCTCTGTCCAATCTTCATTCTGATACTGAAAGGCCATCTGCAAGTGTTGTTACGGCCAACCGTTATATGAGACCTCAACCTTCCAGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGGCCTGTTCGCACAGAAAACACTGTTGCACACTGTGATTTAGAGTCTGCTAGTGCTTTGGAAGTTGCTGAGAATGCTGAGAATGGATCTTCCCATGACCAAACTATACCGGAAAGGTCTCCCGTAAGACCCATGCCTTTGAGTCAGTCCACGTCACAACGCTAG

Protein sequence

MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQGQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSASVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQTIPERSPVRPMPLSQSTSQR
Homology
BLAST of CmoCh10G001360 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 539.3 bits (1388), Expect = 3.6e-152
Identity = 254/354 (71.75%), Postives = 291/354 (82.20%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLA SV++V++NW RY  C +PLHIWI+VDYTTVF+FR+ MFV
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLA+G+GLDFG QQR A FCGR+VVLS+L+LLLYPFLWAWT IGT WF ++  CL E+
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC L+CIA + +GKWLTRRQ  L RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQE R + QDAA YHPGLYLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFH+G+EVRGLPCAHNFHV CID+WLRLNVKCPRCRCSVFP+LDLSALSNL S      +
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 SVVTAN---RYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAEN 352
            V T     RY+R QP S+SY +R+Q L+ PV T+           +ALE AEN
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVHTD-----------TALETAEN 343

BLAST of CmoCh10G001360 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-10
Identity = 50/193 (25.91%), Postives = 86/193 (44.56%), Query Frame = 0

Query: 98  FLWAWTAIGTLWFREAHKCLFEQGQK--WGFLIWLLFS-----YC-ALVCIACVSLGKWL 157
           F + W  IG  W     + L     +  W  +I+L F      +C AL C+  +++   L
Sbjct: 213 FSFIWWIIGFYWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCL 272

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++P + F   G   ++ G+       G+      
Sbjct: 273 PCIIAILY----AVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR-----GIMTECGT 332

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E           +++  + +EC ICL E+  G E+R LPC H+FH  CID+WL +N
Sbjct: 333 DSPIE-----------RSLSPEDAECCICLCEYEDGVELRELPCNHHFHCTCIDKWLHIN 385

Query: 278 VKCPRCRCSVFPN 283
            +CP C+ ++  N
Sbjct: 393 SRCPLCKFNILKN 385

BLAST of CmoCh10G001360 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.4e-10
Identity = 45/196 (22.96%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 98  FLWAWTAIGTLWFREAHKCLFEQGQK--W------GFLIWLLFSYCALVCIACVSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +    +A     G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 343 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 402

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 403 ATCPLCKYNILKSSNL 403

BLAST of CmoCh10G001360 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.8e-10
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 227 AVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSV 280
           A+P + +EC ICL  +    E+R LPC H+FH GC+D+WL +N  CP C+ ++
Sbjct: 317 ALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

BLAST of CmoCh10G001360 vs. ExPASy Swiss-Prot
Match: Q9SK92 (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-10
Identity = 31/73 (42.47%), Postives = 39/73 (53.42%), Query Frame = 0

Query: 215 ALIEELPKFRLKAVPT-----DCSECPICLEEFHVGNEVRGLP-CAHNFHVGCIDEWLRL 274
           ++IE  P F    V T     +  ECP+CL EF     +R +P C H FH GCID WLR 
Sbjct: 93  SVIETFPTFPYSTVKTLRIGKEALECPVCLNEFEDDETLRLIPQCCHVFHPGCIDAWLRS 152

Query: 275 NVKCPRCRCSVFP 282
              CP CR ++ P
Sbjct: 153 QTTCPLCRANLVP 165

BLAST of CmoCh10G001360 vs. ExPASy TrEMBL
Match: A0A6J1E7C4 (E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430080 PE=4 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 8.1e-224
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ
Sbjct: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQR 381
           TIPERSPVRPMPLSQSTSQR
Sbjct: 361 TIPERSPVRPMPLSQSTSQR 380

BLAST of CmoCh10G001360 vs. ExPASy TrEMBL
Match: A0A6J1JJE6 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485643 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.1e-217
Identity = 370/379 (97.63%), Postives = 373/379 (98.42%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDC ECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCCECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYMRPQPSSQSYLVRLQGLLRPV TENTVAH DLESASALEVAENAENGSSHDQ
Sbjct: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVHTENTVAHGDLESASALEVAENAENGSSHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQ 380
           T+PERSPVRP+  SQST Q
Sbjct: 361 TVPERSPVRPVSSSQSTPQ 379

BLAST of CmoCh10G001360 vs. ExPASy TrEMBL
Match: A0A1S3BVE9 (E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.0e-205
Identity = 352/379 (92.88%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQG PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGFPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELP+FRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPEFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRY+  QPSSQSYLVRLQGLLRPVRTEN  AH DL  ASALE    AENGS+HDQ
Sbjct: 301 SVVTANRYVGSQPSSQSYLVRLQGLLRPVRTENNDAHGDLGPASALEA---AENGSAHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQ 380
           TIPERSPVRP+ L QSTSQ
Sbjct: 361 TIPERSPVRPLSLIQSTSQ 376

BLAST of CmoCh10G001360 vs. ExPASy TrEMBL
Match: A0A0A0LS35 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.8e-205
Identity = 350/379 (92.35%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWKRYHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKRYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LA+LLYPFLWAWTAIGT+WFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLAVLLYPFLWAWTAIGTIWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDM+RVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMVRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SV+TANRY+  QPSSQSYLVRLQGLLRPVRTE   AH DL  ASALE    AENGS+H+Q
Sbjct: 301 SVLTANRYVGSQPSSQSYLVRLQGLLRPVRTEENDAHGDLGPASALEA---AENGSAHEQ 360

Query: 361 TIPERSPVRPMPLSQSTSQ 380
           TIPERSPVRPM L QSTSQ
Sbjct: 361 TIPERSPVRPMSLIQSTSQ 376

BLAST of CmoCh10G001360 vs. ExPASy TrEMBL
Match: A0A6J1F591 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440966 PE=4 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 3.7e-200
Identity = 344/381 (90.29%), Postives = 356/381 (93.44%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLA SVV+VSI+WKRYHTCVHPLHIWI+VDYT VF+FRLLMFV
Sbjct: 1   MATRGVDFKWYDGFFLSMLATSVVIVSISWKRYHTCVHPLHIWIVVDYTAVFLFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLL+PFLW WTAIGTLWF++AH CL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLFPFLWVWTAIGTLWFKQAHNCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAY--HPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC 240
           AFEAAG ETRVMGQDAA Y  HPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC
Sbjct: 181 AFEAAGIETRVMGQDAATYHPHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC 240

Query: 241 LEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERP 300
           LEEFH GNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RP
Sbjct: 241 LEEFHEGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRP 300

Query: 301 SASVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSH 360
           SASVVTAN YM   P SQSYLVRLQGLLRPVRTENT  H DLESASA+EV    ENGS+H
Sbjct: 301 SASVVTANSYMISHPLSQSYLVRLQGLLRPVRTENTGTHGDLESASAVEV---TENGSAH 360

Query: 361 DQTIPERSPVRPMPLSQSTSQ 380
           DQTIPERSPVRPMPLSQST Q
Sbjct: 361 DQTIPERSPVRPMPLSQSTPQ 378

BLAST of CmoCh10G001360 vs. NCBI nr
Match: XP_022921995.1 (E3 ubiquitin-protein ligase SIS3-like [Cucurbita moschata])

HSP 1 Score: 785.8 bits (2028), Expect = 1.7e-223
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ
Sbjct: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQR 381
           TIPERSPVRPMPLSQSTSQR
Sbjct: 361 TIPERSPVRPMPLSQSTSQR 380

BLAST of CmoCh10G001360 vs. NCBI nr
Match: XP_023516682.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.0 bits (2000), Expect = 3.0e-220
Identity = 376/380 (98.95%), Postives = 376/380 (98.95%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAH DLESA ALE AENAENGSSHDQ
Sbjct: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHGDLESAGALEAAENAENGSSHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQR 381
           TIPERSPVRPM LSQSTSQR
Sbjct: 361 TIPERSPVRPMSLSQSTSQR 380

BLAST of CmoCh10G001360 vs. NCBI nr
Match: XP_022988385.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 765.4 bits (1975), Expect = 2.3e-217
Identity = 370/379 (97.63%), Postives = 373/379 (98.42%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDC ECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCCECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYMRPQPSSQSYLVRLQGLLRPV TENTVAH DLESASALEVAENAENGSSHDQ
Sbjct: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVHTENTVAHGDLESASALEVAENAENGSSHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQ 380
           T+PERSPVRP+  SQST Q
Sbjct: 361 TVPERSPVRPVSSSQSTPQ 379

BLAST of CmoCh10G001360 vs. NCBI nr
Match: KAG6589451.1 (E3 ubiquitin-protein ligase SIS3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 747.7 bits (1929), Expect = 5.1e-212
Identity = 361/364 (99.18%), Postives = 361/364 (99.18%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYH CVHPLHIWILVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHPCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTANRYM PQPSSQSYLVRLQGLLRPVRTENTVAH DLESASALEVAENAENGSSHDQ
Sbjct: 301 SVVTANRYMSPQPSSQSYLVRLQGLLRPVRTENTVAHGDLESASALEVAENAENGSSHDQ 360

Query: 361 TIPE 365
           TIPE
Sbjct: 361 TIPE 364

BLAST of CmoCh10G001360 vs. NCBI nr
Match: XP_038880106.1 (E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida])

HSP 1 Score: 727.2 bits (1876), Expect = 7.1e-206
Identity = 355/379 (93.67%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWKRYHTCVHPLHIWI+VDYT VFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVMVSINWKRYHTCVHPLHIWIVVDYTAVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SD +RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDVDRPSA 300

Query: 301 SVVTANRYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQ 360
           SVVTAN YMR QPSSQSYLVRLQGLLRPVRTE+T A  DLESA+ALE AENA   S HDQ
Sbjct: 301 SVVTANHYMRSQPSSQSYLVRLQGLLRPVRTESTDAQSDLESANALEAAENA---SIHDQ 360

Query: 361 TIPERSPVRPMPLSQSTSQ 380
           TIP RSPVRPM LSQSTSQ
Sbjct: 361 TIPGRSPVRPMSLSQSTSQ 376

BLAST of CmoCh10G001360 vs. TAIR 10
Match: AT3G47990.1 (SUGAR-INSENSITIVE 3 )

HSP 1 Score: 539.3 bits (1388), Expect = 2.5e-153
Identity = 254/354 (71.75%), Postives = 291/354 (82.20%), Query Frame = 0

Query: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLA SV++V++NW RY  C +PLHIWI+VDYTTVF+FR+ MFV
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120
           DNGLA+G+GLDFG QQR A FCGR+VVLS+L+LLLYPFLWAWT IGT WF ++  CL E+
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC L+CIA + +GKWLTRRQ  L RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQE R + QDAA YHPGLYLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300
           EFH+G+EVRGLPCAHNFHV CID+WLRLNVKCPRCRCSVFP+LDLSALSNL S      +
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 SVVTAN---RYMRPQPSSQSYLVRLQGLLRPVRTENTVAHCDLESASALEVAEN 352
            V T     RY+R QP S+SY +R+Q L+ PV T+           +ALE AEN
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVHTD-----------TALETAEN 343

BLAST of CmoCh10G001360 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 4.4e-12
Identity = 31/72 (43.06%), Postives = 40/72 (55.56%), Query Frame = 0

Query: 207 PAQREAVEALIEELPKFRL--KAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDE 266
           P    A E  I  LP  ++  + +  D S+C +C+EEF VG +   LPC H +H  CI  
Sbjct: 194 PGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVP 253

Query: 267 WLRLNVKCPRCR 277
           WLRLN  CP CR
Sbjct: 254 WLRLNNSCPICR 265

BLAST of CmoCh10G001360 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 69.3 bits (168), Expect = 7.5e-12
Identity = 50/193 (25.91%), Postives = 86/193 (44.56%), Query Frame = 0

Query: 98  FLWAWTAIGTLWFREAHKCLFEQGQK--WGFLIWLLFS-----YC-ALVCIACVSLGKWL 157
           F + W  IG  W     + L     +  W  +I+L F      +C AL C+  +++   L
Sbjct: 213 FSFIWWIIGFYWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCL 272

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++P + F   G   ++ G+       G+      
Sbjct: 273 PCIIAILY----AVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR-----GIMTECGT 332

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E           +++  + +EC ICL E+  G E+R LPC H+FH  CID+WL +N
Sbjct: 333 DSPIE-----------RSLSPEDAECCICLCEYEDGVELRELPCNHHFHCTCIDKWLHIN 385

Query: 278 VKCPRCRCSVFPN 283
            +CP C+ ++  N
Sbjct: 393 SRCPLCKFNILKN 385

BLAST of CmoCh10G001360 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 68.9 bits (167), Expect = 9.7e-12
Identity = 45/196 (22.96%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 98  FLWAWTAIGTLWFREAHKCLFEQGQK--W------GFLIWLLFSYCALVCIACVSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +    +A     G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 343 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 402

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 403 ATCPLCKYNILKSSNL 403

BLAST of CmoCh10G001360 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 68.9 bits (167), Expect = 9.7e-12
Identity = 45/196 (22.96%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 98  FLWAWTAIGTLWFREAHKCLFEQGQK--W------GFLIWLLFSYCALVCIACVSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 152 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 211

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +    +A     G+      
Sbjct: 212 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 271

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 272 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 331

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 332 ATCPLCKYNILKSSNL 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GYT93.6e-15271.75E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV... [more]
Q93Z921.0e-1025.91E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q9LN711.4e-1022.96E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q8LDB81.8e-1045.28E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q9SK922.3e-1042.47E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E7C48.1e-224100.00E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430... [more]
A0A6J1JJE61.1e-21797.63E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A1S3BVE91.0e-20592.88E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE... [more]
A0A0A0LS353.8e-20592.35RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 P... [more]
A0A6J1F5913.7e-20090.29E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
XP_022921995.11.7e-223100.00E3 ubiquitin-protein ligase SIS3-like [Cucurbita moschata][more]
XP_023516682.13.0e-22098.95E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022988385.12.3e-21797.63E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita maxima][more]
KAG6589451.15.1e-21299.18E3 ubiquitin-protein ligase SIS3, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_038880106.17.1e-20693.67E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G47990.12.5e-15371.75SUGAR-INSENSITIVE 3 [more]
AT1G60360.14.4e-1243.06RING/U-box superfamily protein [more]
AT4G11680.17.5e-1225.91Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.19.7e-1222.96Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.29.7e-1222.96Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 235..275
e-value: 5.4E-7
score: 39.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 234..276
e-value: 3.3E-13
score: 49.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 235..276
score: 12.622055
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 204..281
e-value: 3.9E-19
score: 70.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 349..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 349..363
NoneNo IPR availablePANTHERPTHR47179:SF1E3 UBIQUITIN-PROTEIN LIGASE SIS3coord: 1..354
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 234..278
e-value: 2.15537E-18
score: 76.1961
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 216..284
IPR044793E3 ubiquitin-protein ligase SIS3PANTHERPTHR47179E3 UBIQUITIN-PROTEIN LIGASE SIS3coord: 1..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh10G001360.1CmoCh10G001360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0010182 sugar mediated signaling pathway
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity