CmoCh09G013360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh09G013360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDLH domain-containing protein
LocationCmo_Chr09: 12113553 .. 12115087 (-)
RNA-Seq ExpressionCmoCh09G013360
SyntenyCmoCh09G013360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATGAAAGATATTTGTTTTGCACTGCAAATCTTCTCTTGGCTAGAGAGAGAGAGAGAATGGGATCGGCCACCGCCGGAGCTCAGTGGATTTCCGCCCCACCGACCGGGAGAAGTGGCCGACGCATGGGGATCCCATTTCAACCTTCCTTCCGATTTGCTTTTCCGATTAGCAATAGTATTGGGCAGTTGAAGGGCAAGAGAGAGATAAAGGGAGGCAAGGTGTGTTACAGCGCAGAGGGAGTGAAGGATGAGATAGAGGAGGAGGAGGCTTGTGAGCTTGTGAATGGAATGGAGCTTTCCATTGGAGAAGAAGCTCACCATTCAATCACTGCATATCTCTTCAAGGCAGTCAAGAACAATAATGGAACAGCTATTCTGCTCTTGACGGATCTCTTTGCCTTTCAAGACTCTTCTGTTCGAGACTTCGCCTATCGTCTCGCTTGCAATGGCTTCAAGTATGCTATCTAACCTTATGCTCCCTTCAAGCTTTTTCATTATAATTCATATCTTTGTTAAGAACCACAACTCTCCACAATTATATGATATTGTCCACTTTGAGCTTAAGCTCTCATAGTTTTGCTCTGTGCTTTCCTAAAAAGACTCACGTCAACAAAGATATATTCCTTACCTATAAACACATGATCGTGGGACTCTCTCTCTCTCTCGATCAGGCTTTTAATGTTTTCTCAACTTGAACTACAGTGTCTTGCTTCCAGATGTATTTCATGGAGATTCATGTGAGGAACAAAAGACTGTGGAAGACATAGCAGCAGCAACAAAATGGTTGGTTGACGAGTTTTCAGCAGCAGGGATCTCGAGGAAGCTTGGGATCGTGGGATTTGGGTACGGGGGAGGCCGGGTAATCGACGTTCTAGCTCAAGACAAGGGTTCCTATTTTTCCATTGGCGCCTCCTTATATGGCACTGAGGTTAATCCTTCTCTGGCTACCAAAGTCGAGGTTCCAGTATTGCTCATCTCAGGGGATAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAGGGGATGGGAGAAGGTTCTAAGTTGGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAAACCTGGATCTCCAGAAGAAGATGAAGATGCTGAGCAGGCTTTTCTTATGCTAAGAAATTGGCTGCATGATGGATTGGTTGCAACTAATATTGAGCCAACTGCATAGGTAATTAGGTAATAAATGAAGTAGCTAACTGGTGCTACTTGGGTTCCAACACGCCTAAATGGGGGATGGCTCACTTTTATGCATTTCAGCAATGGTGTAGCTAATCCTCTGTACCTATTTTTCAGGCTAAAAATGGCAGTTATCAAACAAGACGACTCCCTGCTACCTTAGCTCCCCAGCACTAAGGGGGTCTGGGTCTTTTCTACGAAATCATTGATGATAAACGTGAAAGAAAACATAGAAGCAATAAATTTCATCCTAACAAAGACATTATGGAAGGACATCAACCTAAAAAAGTAAAGTTTCCTTTGGAAAAGCACATAAATCCATTAGC

mRNA sequence

CAAAATGAAAGATATTTGTTTTGCACTGCAAATCTTCTCTTGGCTAGAGAGAGAGAGAGAATGGGATCGGCCACCGCCGGAGCTCAGTGGATTTCCGCCCCACCGACCGGGAGAAGTGGCCGACGCATGGGGATCCCATTTCAACCTTCCTTCCGATTTGCTTTTCCGATTAGCAATAGTATTGGGCAGTTGAAGGGCAAGAGAGAGATAAAGGGAGGCAAGGTGTGTTACAGCGCAGAGGGAGTGAAGGATGAGATAGAGGAGGAGGAGGCTTGTGAGCTTGTGAATGGAATGGAGCTTTCCATTGGAGAAGAAGCTCACCATTCAATCACTGCATATCTCTTCAAGGCAGTCAAGAACAATAATGGAACAGCTATTCTGCTCTTGACGGATCTCTTTGCCTTTCAAGACTCTTCTGTTCGAGACTTCGCCTATCGTCTCGCTTGCAATGGCTTCAATGTCTTGCTTCCAGATGTATTTCATGGAGATTCATGTGAGGAACAAAAGACTGTGGAAGACATAGCAGCAGCAACAAAATGGTTGGTTGACGAGTTTTCAGCAGCAGGGATCTCGAGGAAGCTTGGGATCGTGGGATTTGGGTACGGGGGAGGCCGGGTAATCGACGTTCTAGCTCAAGACAAGGGTTCCTATTTTTCCATTGGCGCCTCCTTATATGGCACTGAGGTTAATCCTTCTCTGGCTACCAAAGTCGAGGTTCCAGTATTGCTCATCTCAGGGGATAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAGGGGATGGGAGAAGGTTCTAAGTTGGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAAACCTGGATCTCCAGAAGAAGATGAAGATGCTGAGCAGGCTTTTCTTATGCTAAGAAATTGGCTGCATGATGGATTGGTTGCAACTAATATTGAGCCAACTGCATAGGTAATTAGGTAATAAATGAAGTAGCTAACTGGTGCTACTTGGGTTCCAACACGCCTAAATGGGGGATGGCTCACTTTTATGCATTTCAGCAATGGTGTAGCTAATCCTCTGTACCTATTTTTCAGGCTAAAAATGGCAGTTATCAAACAAGACGACTCCCTGCTACCTTAGCTCCCCAGCACTAAGGGGGTCTGGGTCTTTTCTACGAAATCATTGATGATAAACGTGAAAGAAAACATAGAAGCAATAAATTTCATCCTAACAAAGACATTATGGAAGGACATCAACCTAAAAAAGTAAAGTTTCCTTTGGAAAAGCACATAAATCCATTAGC

Coding sequence (CDS)

ATGGGATCGGCCACCGCCGGAGCTCAGTGGATTTCCGCCCCACCGACCGGGAGAAGTGGCCGACGCATGGGGATCCCATTTCAACCTTCCTTCCGATTTGCTTTTCCGATTAGCAATAGTATTGGGCAGTTGAAGGGCAAGAGAGAGATAAAGGGAGGCAAGGTGTGTTACAGCGCAGAGGGAGTGAAGGATGAGATAGAGGAGGAGGAGGCTTGTGAGCTTGTGAATGGAATGGAGCTTTCCATTGGAGAAGAAGCTCACCATTCAATCACTGCATATCTCTTCAAGGCAGTCAAGAACAATAATGGAACAGCTATTCTGCTCTTGACGGATCTCTTTGCCTTTCAAGACTCTTCTGTTCGAGACTTCGCCTATCGTCTCGCTTGCAATGGCTTCAATGTCTTGCTTCCAGATGTATTTCATGGAGATTCATGTGAGGAACAAAAGACTGTGGAAGACATAGCAGCAGCAACAAAATGGTTGGTTGACGAGTTTTCAGCAGCAGGGATCTCGAGGAAGCTTGGGATCGTGGGATTTGGGTACGGGGGAGGCCGGGTAATCGACGTTCTAGCTCAAGACAAGGGTTCCTATTTTTCCATTGGCGCCTCCTTATATGGCACTGAGGTTAATCCTTCTCTGGCTACCAAAGTCGAGGTTCCAGTATTGCTCATCTCAGGGGATAATGATCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAGGGGATGGGAGAAGGTTCTAAGTTGGTGATATTTGGGGGAAGAGGCCATGGCTTTGTTCACAAACCTGGATCTCCAGAAGAAGATGAAGATGCTGAGCAGGCTTTTCTTATGCTAAGAAATTGGCTGCATGATGGATTGGTTGCAACTAATATTGAGCCAACTGCATAG

Protein sequence

MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNSIGQLKGKREIKGGKVCYSAEGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEPTA
Homology
BLAST of CmoCh09G013360 vs. ExPASy Swiss-Prot
Match: Q96DG6 (Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 6.7e-13
Identity = 66/240 (27.50%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 72  CELVNGMEL-SIGEEAH-HSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDFAYRLAC 131
           C++ + +E   +G E     I AY+ K+   + G A++++ D+F +Q  + R  A  ++ 
Sbjct: 10  CDIGHRLEYGGLGREVQVEHIKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYIADMISG 69

Query: 132 NGFNVLLPDVFHGD-----------------SCEEQKTVEDIAAATKWLVDEFSAAGISR 191
           NG+  ++PD F G                  +   QK   +I+A  K+L  +  A    +
Sbjct: 70  NGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----Q 129

Query: 192 KLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLC 251
           K+GIVGF +GG  V  ++   K S F  G S+YG   +      ++ P L I  +ND + 
Sbjct: 130 KIGIVGFCWGGTAVHHLMM--KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVI 189

Query: 252 PVSVMKDLEKGMGEGSK----LVIFGGRGHGFVHKPG---SPEEDEDAEQAFLMLRNWLH 286
           P+  +  L + + E  K    +  F G+ HGFVH+     SP +    ++A   L  WL+
Sbjct: 190 PLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of CmoCh09G013360 vs. ExPASy Swiss-Prot
Match: Q5RBU3 (Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-12
Identity = 66/240 (27.50%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 72  CELVNGMEL-SIGEEAH-HSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDFAYRLAC 131
           C++ + +E   +G E     I AY+ K+   + G A++++ D+F +Q  + R  A  ++ 
Sbjct: 10  CDIGHRLEYGGLGREVQVEHIKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYMADMISG 69

Query: 132 NGFNVLLPDVFHGD-----------------SCEEQKTVEDIAAATKWLVDEFSAAGISR 191
           NG+  ++PD F G                  +   QK   +I+A  K+L  +  A    +
Sbjct: 70  NGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----Q 129

Query: 192 KLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLC 251
           K+GIVGF +GG  V  ++   K S F  G S+YG   +      ++ P L I  +ND + 
Sbjct: 130 KIGIVGFCWGGIAVHHLMM--KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVI 189

Query: 252 PVSVMKDLEKGMGEGSK----LVIFGGRGHGFVHKPG---SPEEDEDAEQAFLMLRNWLH 286
           P+  +  L + + E  K    +  F G+ HGFVH+     SP +    ++A   L  WL+
Sbjct: 190 PLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of CmoCh09G013360 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.2e-11
Identity = 66/248 (26.61%), Postives = 110/248 (44.35%), Query Frame = 0

Query: 68  EEEACELVNGMELSIGEEAH----HSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDF 127
           E   C    G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + R  
Sbjct: 4   EANPCPCDIGHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNTRYM 63

Query: 128 AYRLACNGFNVLLPDVFHGD-----------------SCEEQKTVEDIAAATKWLVDEFS 187
           A  +A NG+  ++PD F G                  S   +K   ++ A  ++L  +  
Sbjct: 64  ADMIARNGYTTIVPDFFVGQEPWDPAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQQCH 123

Query: 188 AAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSI--GASLYGTEVNPSLATKVEVPVLLI 247
           A    +K+GIVGF +GG  V  V+     +Y  I  G S+YG   +      ++ P L I
Sbjct: 124 A----QKIGIVGFCWGGVVVHQVMT----AYPDIRAGVSVYGIIRDSEDVYNLKNPTLFI 183

Query: 248 SGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFVHKPG---SPEEDEDAEQAF 286
             +ND + P+  +  L + + E      ++  F G+ HGFVH+     SP +    E+A 
Sbjct: 184 FAENDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEAR 242

BLAST of CmoCh09G013360 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.8e-10
Identity = 57/209 (27.27%), Postives = 97/209 (46.41%), Query Frame = 0

Query: 103 GTAILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGD-----------------SC 162
           G A++++ D+F +Q S+ R  A  +A NG+  ++PD F G                  S 
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWDPAGDWSTFPEWLKSR 101

Query: 163 EEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSI--GAS 222
             +K   ++ A  ++L  +  A    +K+GIVGF +GG  V  V+     +Y  +  G S
Sbjct: 102 NARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVVHHVMT----TYPEVRAGVS 161

Query: 223 LYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHG 282
           +YG   +      ++ P L I  +ND + P+  +  L + + E      ++  F G+ HG
Sbjct: 162 VYGIIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHG 221

Query: 283 FVHKPG---SPEEDEDAEQAFLMLRNWLH 286
           FVH+     SP +    E+A   L  WL+
Sbjct: 222 FVHRKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of CmoCh09G013360 vs. ExPASy Swiss-Prot
Match: B8M9K0 (Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=tropI PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 8.8e-05
Identity = 38/179 (21.23%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 106 ILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQK---------------- 165
           +L+LTD+   + S+ +  A + A NG+ V++PD+F GD+    K                
Sbjct: 46  VLILTDIVGHRFSNAQIIADQFAENGYFVMMPDLFLGDAVPLNKPGEFDMGKWRSGAYHP 105

Query: 166 -----TVEDIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASL 225
                  E +    +  + E  +    +K+G VG+ +GG  V+  L   K     +G + 
Sbjct: 106 QGKNHLPETVDPIVEVCLSEMRSKYQCKKIGAVGYCFGGKYVVRHLIPGK---MDVGYTA 165

Query: 226 YGTEVNPSLATKVEVPVLLISGDNDPLCPVS---VMKDLEKGMGEGSKLVIFGGRGHGF 261
           + + ++ S    ++ P+ + +   D + P     V +++ + +G   ++ ++ G  HGF
Sbjct: 166 HPSHIDESELKGIKGPLAIAAAAKDNIFPAEKRHVSEEILQEVGFPYQINLYSGVSHGF 221

BLAST of CmoCh09G013360 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.9e-168
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNSIGQLKGKREIKGGKVCYSAE 60
           MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNSIGQLKGKREIKGGKVCYSAE
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNSIGQLKGKREIKGGKVCYSAE 60

Query: 61  GVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSV 120
           GVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSV
Sbjct: 61  GVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSV 120

Query: 121 RDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFG 180
           RDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFG
Sbjct: 121 RDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFG 180

Query: 181 YGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL 240
           YGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL
Sbjct: 181 YGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL 240

Query: 241 EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEPTA 298
           EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEPTA
Sbjct: 241 EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEPTA 297

BLAST of CmoCh09G013360 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.4e-159
Identity = 282/297 (94.95%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNSIGQLKGKREIKGGKVCYSAE 60
           MGSA+AGAQWISAPPTGRSGRRMGIPFQPSFR AFPISN+IGQLKGKRE KGGKVCYSAE
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQPSFRCAFPISNNIGQLKGKREKKGGKVCYSAE 60

Query: 61  GVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSV 120
           GVKDEI+ EEACELV+GMELSIGEEAHHSITA+LFKAVKNNNGTAILLLTDLF+FQDSSV
Sbjct: 61  GVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDLFSFQDSSV 120

Query: 121 RDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEFSAAGISRKLGIVGFG 180
           RDFAYRLACNGFNVLLPDVFHGDSCEEQK VEDI AATKWLVDEF AAGISRKLGIVGFG
Sbjct: 121 RDFAYRLACNGFNVLLPDVFHGDSCEEQKAVEDIGAATKWLVDEFLAAGISRKLGIVGFG 180

Query: 181 YGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL 240
           YGGGRVIDVLAQD+GSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL
Sbjct: 181 YGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDL 240

Query: 241 EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEPTA 298
           EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIE +A
Sbjct: 241 EKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGLVATNIEASA 297

BLAST of CmoCh09G013360 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.8e-114
Identity = 216/304 (71.05%), Postives = 247/304 (81.25%), Query Frame = 0

Query: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPS-FRFAFPISNSIGQLKGKREIKGGKVCYSA 60
           MG A+AGAQ IS PPTG S RR+G PFQP  F+FA+P+ +      G    K GK+ YS 
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWK-GKLRYSV 60

Query: 61  EGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSS 120
           EG   + ++++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFAFQ SS
Sbjct: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120

Query: 121 VRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVDEFSAA 180
           +RDFAYRLAC GFNVLLPD+ H D  E+           Q+  EDI   TKWL +EFSAA
Sbjct: 121 IRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAA 180

Query: 181 GISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGDN 240
           G+S KLGI+GFGYGGGRVIDVLA+D+G+ F IGAS+YGT+V+PSLATKV+VPVLLISGDN
Sbjct: 181 GLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDN 240

Query: 241 DPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDGL 293
           DPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVH PGSPEED+DAE+AFLMLRNWLH GL
Sbjct: 241 DPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGL 300

BLAST of CmoCh09G013360 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 4.1e-114
Identity = 220/305 (72.13%), Postives = 249/305 (81.64%), Query Frame = 0

Query: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPISNSIGQLKGKREIKGGKVCYSA 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+ +      G  + KG    YS 
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDS 120
           +G VKD  ++++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFAFQDS
Sbjct: 61  KGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDS 120

Query: 121 SVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVDEFSA 180
           S+RDFAYRLACNGFNVLLPD+ + D  E+           Q+  EDI   TKWL DEFSA
Sbjct: 121 SIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSA 180

Query: 181 AGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 240
           AG+SRKLGI+GFG+GGGRVIDVLA+DKG+ F IGASLYGTEV+PSLATKV+VPVLLISGD
Sbjct: 181 AGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGD 240

Query: 241 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 293
           +DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRNWLH G
Sbjct: 241 DDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHG 300

BLAST of CmoCh09G013360 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 4.1e-114
Identity = 220/305 (72.13%), Postives = 249/305 (81.64%), Query Frame = 0

Query: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPISNSIGQLKGKREIKGGKVCYSA 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+ +      G  + KG    YS 
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  EG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDS 120
           +G VKD  ++++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFAFQDS
Sbjct: 61  KGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDS 120

Query: 121 SVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVDEFSA 180
           S+RDFAYRLACNGFNVLLPD+ + D  E+           Q+  EDI   TKWL DEFSA
Sbjct: 121 SIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSA 180

Query: 181 AGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 240
           AG+SRKLGI+GFG+GGGRVIDVLA+DKG+ F IGASLYGTEV+PSLATKV+VPVLLISGD
Sbjct: 181 AGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGD 240

Query: 241 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 293
           +DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRNWLH G
Sbjct: 241 DDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHG 300

BLAST of CmoCh09G013360 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.1e-74
Identity = 130/242 (53.72%), Postives = 175/242 (72.31%), Query Frame = 0

Query: 64  DEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDF 123
           D+ EEEEACELVNG E+S+       +  YL  AVKNNNGT +LLL+D+F FQDS+ RDF
Sbjct: 74  DDDEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDF 133

Query: 124 AYRLACNGFNVLLPDVFHGDSCEEQKTV----------------EDIAAATKWLVDEFSA 183
           AYR+ACNG+NVL+PD+F GD   + +                  +D    TKW+V+EF+A
Sbjct: 134 AYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAA 193

Query: 184 AGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 243
           AGIS+KLG++GF +GGGRV+DVLA D+  YFS G S YGT ++ ++A  V+VPVL I+GD
Sbjct: 194 AGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGD 253

Query: 244 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 290
            DPLC V  + ++E+ +GEGSK+V++ GRGHGFVH+P +PE+D DAE+AF ++RNWLH  
Sbjct: 254 RDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHH 310

BLAST of CmoCh09G013360 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.1e-74
Identity = 130/242 (53.72%), Postives = 175/242 (72.31%), Query Frame = 0

Query: 64  DEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFAFQDSSVRDF 123
           D+ EEEEACELVNG E+S+       +  YL  AVKNNNGT +LLL+D+F FQDS+ RDF
Sbjct: 79  DDDEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDF 138

Query: 124 AYRLACNGFNVLLPDVFHGDSCEEQKTV----------------EDIAAATKWLVDEFSA 183
           AYR+ACNG+NVL+PD+F GD   + +                  +D    TKW+V+EF+A
Sbjct: 139 AYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAA 198

Query: 184 AGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 243
           AGIS+KLG++GF +GGGRV+DVLA D+  YFS G S YGT ++ ++A  V+VPVL I+GD
Sbjct: 199 AGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGD 258

Query: 244 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 290
            DPLC V  + ++E+ +GEGSK+V++ GRGHGFVH+P +PE+D DAE+AF ++RNWLH  
Sbjct: 259 RDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHH 315

BLAST of CmoCh09G013360 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 5.6e-07
Identity = 49/202 (24.26%), Postives = 88/202 (43.56%), Query Frame = 0

Query: 105 AILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQK------------TVE 164
           A+LL+  +F ++  ++R  A ++A  GF  ++PD FHGD    +              +E
Sbjct: 41  AVLLVPHVFGYETPNLRKLADKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELE 100

Query: 165 DIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPS 224
                +K +V+     GI+  +G  GF + G +V   LA++K         L+   V   
Sbjct: 101 KGFEESKPIVEALKNKGIT-SIGAAGFCW-GAKVAVELAKEK--LVDATVLLHPARVTVD 160

Query: 225 LATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGS 284
              +V +P+ ++  + D + P  +++  E  +       S + IF    HG+   +    
Sbjct: 161 DIKEVNLPIAVLGAEIDQVSPPELVRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNEND 220

Query: 285 PEEDEDAEQAFLMLRNWLHDGL 289
           P E E A +A   +  WL D L
Sbjct: 221 PSEVEAAMEAHKDMLAWLIDYL 238

BLAST of CmoCh09G013360 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 7.4e-07
Identity = 44/193 (22.80%), Postives = 79/193 (40.93%), Query Frame = 0

Query: 106 ILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAAATKWLVDEF 165
           +LL++D+F F+  ++R  A ++A +GF V++PD F GD          I     W+ D  
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPV---WIKDHG 101

Query: 166 SAAGIS---------RKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATK 225
              G           +  GI   G   G    V+   K         L+ + VN      
Sbjct: 102 CDKGFENTKPVLETIKNKGITAIG-AAGMCWGVVELSKEELIQAAVLLHPSFVNVDDIKG 161

Query: 226 VEVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGSPEED 284
            + P+ ++  + D + P +++K  E+ +       S + I     HG+   +    PE  
Sbjct: 162 GKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAV 221

BLAST of CmoCh09G013360 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 9.6e-07
Identity = 44/196 (22.45%), Postives = 85/196 (43.37%), Query Frame = 0

Query: 106 ILLLTDLFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQKTVEDIAA--------- 165
           +LL++D+F F+  ++R  A ++A +GF V++PD F GD          I           
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 166 ---ATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSL 225
               TK +++     GI+  +G  G  +G   V+++    K         L+ + VN   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWGAKVVVEL---SKEELIQAAVLLHPSFVNVDD 161

Query: 226 ATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGSP 284
               + P+ ++  + D + P +++K  E+ +       S + I     HG+   +    P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q96DG66.7e-1327.50Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1[more]
Q5RBU31.1e-1227.50Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1[more]
Q8R1G28.2e-1126.61Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1[more]
Q7TP521.8e-1027.27Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 ... [more]
B8M9K08.8e-0521.23Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6... [more]
Match NameE-valueIdentityDescription
A0A6J1EC402.9e-168100.00carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC1114327... [more]
A0A6J1I9081.4e-15994.95carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595... [more]
A0A0A0KT041.8e-11471.05DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV... [more]
A0A5A7TB034.1e-11472.13Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CB894.1e-11472.13uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=... [more]
Match NameE-valueIdentityDescription
AT1G35420.13.1e-7453.72alpha/beta-Hydrolases superfamily protein [more]
AT1G35420.23.1e-7453.72alpha/beta-Hydrolases superfamily protein [more]
AT3G23570.15.6e-0724.26alpha/beta-Hydrolases superfamily protein [more]
AT3G23600.27.4e-0722.80alpha/beta-Hydrolases superfamily protein [more]
AT3G23600.19.6e-0722.45alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 78..287
e-value: 9.7E-34
score: 118.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 89..286
IPR002925Dienelactone hydrolasePFAMPF01738DLHcoord: 91..285
e-value: 1.0E-16
score: 61.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR17630:SF88DIENELACTONE HYDROLASE FAMILY PROTEINcoord: 51..290
NoneNo IPR availablePANTHERPTHR17630DIENELACTONE HYDROLASEcoord: 51..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh09G013360.1CmoCh09G013360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity