CmoCh09G011940 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh09G011940
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationCmo_Chr09: 8815240 .. 8818889 (-)
RNA-Seq ExpressionCmoCh09G011940
SyntenyCmoCh09G011940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAATAGAAGGTCTCAACCACAGGCCATTAAAAGTGCTGGCAGATGAGGCAATCAAGTTGAGGTTTAATTGTGTACGACTCACATATGCAACTCAAATGTTCACTCGTTATGCAAATAGGACAGTTGAAGAGAATTTTGACCTTCTCGATTTAGAGCAAGCAAAAGCCGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCCTATGAAGCTGTTGTTGATGTGCTAGGAGAGAGTGGTTTAATGGTGATTGCCGACAACCATATGAGTCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGATTTTTTGGAGACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTTCCAAGAAACCAACGGTACGTACAAATTAAGGAAAATTCTCACACTCTTGAAACTAAACTCACACATTTAAAGACTTGTACAATTTTTCACTAACAAAAGAACATTCTTTTGTTATGACATTTAGGTGGTTGGAATGAGCCTACGAAACGAGATACGAGGAACCAATGAAAATGCAAATGATTGGAACAATTATGTTACTCAAGGAGTAACAACAATCCACAACATTAACCCAAATGTTTTAGTGATTGTTTCGGGCCTAAATTTTGATAATGATCTTCGATGCTTAAAAGAGAAGCCCTTGAACGTAAGCACCTTAGACAACAAGTTGGTTTTTGAGGTGCACTTGTATTCGTTCAGTGGAGATCAAGAGAGTAAATTTATAAACCAGCCACTAAATAATATTTGTGCAAATATTATCAATGGCTTTGTAGACCATGCTGAATTTGTTAGAGAAGGACCAAACCCATTTCCTTTGTTTGTCAGCGAATATGGGTACGATCAGAGAGAGGTTAACCATGCAGAAAATCGGTTCATGAGTTGCTTCACAGCTCATCTTGCACAAGAAGATTTAGATTGGGCATTATGGACTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAACAGGGCCGGCAGAAACTTTTGGAGTTCTCGACTCCAATTGGACTCAAATTAAAAATCCTAACTTTGTCCAGAAGTTTCAACTATTGCAGACTATGTTGCAAGGTATGTATAAATATATTAACGATTTTGAATATATGGATCTATAACAAGTGTATAAGACATTTTTTTACGGTGCACAGATCCAAATTCCAATGCATCGTTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCAAATGACAACAAAGATATTTTCATGGGTAATTGCTCCATCTCAAGTCGATGGACTCATGACAATGATAGCACTCCAATTAGGATGTCAAGCACTGGTTTATGCTTAAAGACGAGCGGAGAAGGCCTTATGCCATCTCTCTCAACAGATTGCTTCGGCCCTCAGAGTTCTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCGTAACTCAAGATGGGAAGAGTCTTTGCTTGCAGGTTGAAAGCTCTAATTCTTCAAAAATTGTGACAAACTCATGTATTTGCACTGACGGTGCTCCAAATTGCCTCCAAGACACCCAAAGTCAATGGTTTGAACTCGTTGAAACCAACACATTGTGAGTTGCTTATGTATTGGGAAGAATAAATATATGACATGCCCGACTTTGAATTGGCCCCTATTTTATCTCTAATTCTATTCATCCTCGTGCTTAATTATAACCGATTTTATTTATCGTACAAACATATATGGAAAATAATACCAACAATAATGATCGTATAAAAGATTGGACATATAATAAATAATAGAAAATAATTATTGACCATTTAACTACGAATTTAAGCATAAAATCGACAACCCTAATATGACTCCCGCACTTTCAAAACTCTCATGCCAACCCTAAATTCAAATTCCAACAACTACTAGTATATTGGACCAAATTCTAAATTTCTATTTTAAACTAATTAATAGACTAAGCAACATTTGTTGGAACAAGTTTGAACCATTGGGGTTGAGGATCATCCGNAAACAAATCTGAGAAATATTTAAACTAAATTTCTTAAAAGACTGTAACTCTTTTTCATCTAATCAAATTCTTCTGACCATTTCTCCCGTGAACTTATTCTTCTTTGTCAAGAATTAGTTCAACTCAGAAAAATCTGCACGCTAAGTTCTTCACGCAACTTTCGAAATGCTTTCATATGTGTTTGGAACGTCCGTTTAGAACTGGATTTTTGGATAGCTTAATAGTTGAAGTATTACACACATTAGCTTTGTTCCTTCTATTTGAGAGTGACAAATCTCTTTGAGTATCCTCCTCGTCCAAACAGCTTGACATGCACAGGAACTAGCAGCAACGAACTCAGCTTCTGTGGTTCATAAGGTGACTATAGGCTGCTTACGAGAAGACCAAGCTACTGCTCCTCCACTAATCACAAGCACATGGCCTGAAGTACTCTTGCTTTCTTCCATGTCTCCGGCACAGTCACTACCAGTCAATCAAGTCAAATCACCGGCTCCTCCTTTTTTATAAAATACACCATAATCTACCATACCTTTCAAGTACCTTAAAACCGTTTTACCTACTGCAAAATGTTGTTGTGTTGGACATGCCATAAAACGACTAAACAAACTTACAGCAAGCATGAGATCAAGGCAAATAGTTGTGAGATACATCAGACTTCCCACTATTTGTTTATACAAAGTTACATATACTGTTGCACCATTTTCATCTTTGCCAATTTTCTTTCCTGGAACAGTGGGATTACAAACAGAATTGTAATTCTCTATGCCATGTCGGTTTAAAACTTCAACAGCATACTTTCTTTGACATGTGAAAATAGGCATCTAATCTTTGTATTACCTCTATTCCAAGAAAAAATCTCATCTGACCTAAGTCAGTCATATCAAACTCTTTCTTCAAGGAACGTTTAAACTCGTCCAATAGTTCTTCATCTGTTCTCCAGTAAATAAAAAATCATCAACATAAATGCTTACAATAAGAATTTTACCTCCCTTCCTCTTGATGAAGAGTGTTTGCTCACTGGAACTCCTTTTGAATCCTTCCTTGTTTTAATCCGTAGAGAGACTTTTAGAGTTTGTACACCATCTCCTCACTTCCCTTTTTCTCATAGCCTTCACCGGAATCTGCTAACCATACAGGTGGATGTCGATTTCTCCCCTCCCTTACTGTCAAATCTGGTGCGACGGTCACTGGAAAAATTATTTTCAGCAACTGGATCAATTGTTTCAGCAGCCGATTCAGTCGTTTCTTCCGTGATTTTTCTTTCTCCGTTTTCAGCAACTGGTTCAATTGTTTCAGCAACCGGTTCAGTCGTTTCCTCTGTTATTCCTCCTCCTTCGACTGTGTTCTTATCAATTTCAATGTTTTCCCCCAGTCTAATTTTTCCATTGCTCTCCATTTACCCATTTTTTGAAATGGAGTCCGATGTTGCTTGCCTTTCATACATGCCTCATAGGTGGTGTTTGGAGCCCCAATTTGCGGCAGGCCTTTCACCATGTTTTTGTGTTTCAAGGTCCACAAGCCTTTGTAGCCGAGATGACCATATCGATGGTGCCGAAGTGTTGACTGGTCCGTATTTGAAACTTGGAGGCATCTTCCTTCATTTGTTGTGGTAG

mRNA sequence

ATGCTAATAGAAGGTCTCAACCACAGGCCATTAAAAGTGCTGGCAGATGAGGCAATCAAGTTGAGGTTTAATTGTGTACGACTCACATATGCAACTCAAATGTTCACTCGTTATGCAAATAGGACAGTTGAAGAGAATTTTGACCTTCTCGATTTAGAGCAAGCAAAAGCCGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCCTATGAAGCTGTTGTTGATGTGCTAGGAGAGAGTGGTTTAATGGTGATTGCCGACAACCATATGAGTCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGATTTTTTGGAGACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTTCCAAGAAACCAACGGTGGTTGGAATGAGCCTACGAAACGAGATACGAGGAACCAATGAAAATGCAAATGATTGGAACAATTATGTTACTCAAGGAGTAACAACAATCCACAACATTAACCCAAATGTTTTAGTGATTGTTTCGGGCCTAAATTTTGATAATGATCTTCGATGCTTAAAAGAGAAGCCCTTGAACGTAAGCACCTTAGACAACAAGTTGGTTTTTGAGGTGCACTTGTATTCGTTCAGTGGAGATCAAGAGAGTAAATTTATAAACCAGCCACTAAATAATATTTGTGCAAATATTATCAATGGCTTTGTAGACCATGCTGAATTTGTTAGAGAAGGACCAAACCCATTTCCTTTGTTTGTCAGCGAATATGGGTACGATCAGAGAGAGGTTAACCATGCAGAAAATCGGTTCATGAGTTGCTTCACAGCTCATCTTGCACAAGAAGATTTAGATTGGGCATTATGGACTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAACAGGGCCGGCAGAAACTTTTGGAGTTCTCGACTCCAATTGGACTCAAATTAAAAATCCTAACTTTGTCCAGAAGTTTCAACTATTGCAGACTATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCAAATGACAACAAAGATATTTTCATGGGTAATTGCTCCATCTCAAGTCGATGGACTCATGACAATGATAGCACTCCAATTAGGATGTCAAGCACTGGTTTATGCTTAAAGACGAGCGGAGAAGGCCTTATGCCATCTCTCTCAACAGATTGCTTCGGCCCTCAGAGTTCTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCGTAACTCAAGATGGGAAGAGTCTTTGCTTGCAGGTGGATGTCGATTTCTCCCCTCCCTTACTGTCAAATCTGGTGCGACGGTCACTGGAAAAATTATTTTCAGCAACTGGATCAATTGTTTCAGCAGCCGATTCAGTCGTTTCTTCCGTGATTTTTCTTTCTCCGTTTTCAGCAACTGGTTCAATTGTTTCAGCAACCGGTTCAGTCGTTTCCTCTGTTATTCCTCCTCCTTCGACTGTGTTCTTATCAATTTCAATGTTTTCCCCCAGTCTAATTTTTCCATTGCTCTCCATTTACCCATTTTTTGAAATGGAGTCCGATGTTGCTTGCCTTTCATACATGCCTCATAGGTGGTGTTTGGAGCCCCAATTTGCGGCAGGCCTTTCACCATGTTTTTGTGTTTCAAGGTCCACAAGCCTTTGTAGCCGAGATGACCATATCGATGGTGCCGAAGTGTTGACTGGTCCGTATTTGAAACTTGGAGGCATCTTCCTTCATTTGTTGTGGTAG

Coding sequence (CDS)

ATGCTAATAGAAGGTCTCAACCACAGGCCATTAAAAGTGCTGGCAGATGAGGCAATCAAGTTGAGGTTTAATTGTGTACGACTCACATATGCAACTCAAATGTTCACTCGTTATGCAAATAGGACAGTTGAAGAGAATTTTGACCTTCTCGATTTAGAGCAAGCAAAAGCCGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCCTATGAAGCTGTTGTTGATGTGCTAGGAGAGAGTGGTTTAATGGTGATTGCCGACAACCATATGAGTCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGATTTTTTGGAGACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTTCCAAGAAACCAACGGTGGTTGGAATGAGCCTACGAAACGAGATACGAGGAACCAATGAAAATGCAAATGATTGGAACAATTATGTTACTCAAGGAGTAACAACAATCCACAACATTAACCCAAATGTTTTAGTGATTGTTTCGGGCCTAAATTTTGATAATGATCTTCGATGCTTAAAAGAGAAGCCCTTGAACGTAAGCACCTTAGACAACAAGTTGGTTTTTGAGGTGCACTTGTATTCGTTCAGTGGAGATCAAGAGAGTAAATTTATAAACCAGCCACTAAATAATATTTGTGCAAATATTATCAATGGCTTTGTAGACCATGCTGAATTTGTTAGAGAAGGACCAAACCCATTTCCTTTGTTTGTCAGCGAATATGGGTACGATCAGAGAGAGGTTAACCATGCAGAAAATCGGTTCATGAGTTGCTTCACAGCTCATCTTGCACAAGAAGATTTAGATTGGGCATTATGGACTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAACAGGGCCGGCAGAAACTTTTGGAGTTCTCGACTCCAATTGGACTCAAATTAAAAATCCTAACTTTGTCCAGAAGTTTCAACTATTGCAGACTATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCAAATGACAACAAAGATATTTTCATGGGTAATTGCTCCATCTCAAGTCGATGGACTCATGACAATGATAGCACTCCAATTAGGATGTCAAGCACTGGTTTATGCTTAAAGACGAGCGGAGAAGGCCTTATGCCATCTCTCTCAACAGATTGCTTCGGCCCTCAGAGTTCTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCGTAACTCAAGATGGGAAGAGTCTTTGCTTGCAGGTGGATGTCGATTTCTCCCCTCCCTTACTGTCAAATCTGGTGCGACGGTCACTGGAAAAATTATTTTCAGCAACTGGATCAATTGTTTCAGCAGCCGATTCAGTCGTTTCTTCCGTGATTTTTCTTTCTCCGTTTTCAGCAACTGGTTCAATTGTTTCAGCAACCGGTTCAGTCGTTTCCTCTGTTATTCCTCCTCCTTCGACTGTGTTCTTATCAATTTCAATGTTTTCCCCCAGTCTAATTTTTCCATTGCTCTCCATTTACCCATTTTTTGAAATGGAGTCCGATGTTGCTTGCCTTTCATACATGCCTCATAGGTGGTGTTTGGAGCCCCAATTTGCGGCAGGCCTTTCACCATGTTTTTGTGTTTCAAGGTCCACAAGCCTTTGTAGCCGAGATGACCATATCGATGGTGCCGAAGTGTTGACTGGTCCGTATTTGAAACTTGGAGGCATCTTCCTTCATTTGTTGTGGTAG

Protein sequence

MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYREGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTAISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSNLVRRSLEKLFSATGSIVSAADSVVSSVIFLSPFSATGSIVSATGSVVSSVIPPPSTVFLSISMFSPSLIFPLLSIYPFFEMESDVACLSYMPHRWCLEPQFAAGLSPCFCVSRSTSLCSRDDHIDGAEVLTGPYLKLGGIFLHLLW
Homology
BLAST of CmoCh09G011940 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 9.5e-99
Identity = 192/465 (41.29%), Postives = 278/465 (59.78%), Query Frame = 0

Query: 2   LIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTR--YANRTVEENFDLLDLEQAKAGL 61
           L EGLN  P+  +A     L FNCVRLTY+  M TR  Y N TV + F  L+L +A +G+
Sbjct: 62  LPEGLNRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGI 121

Query: 62  AQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQ 121
              NP +L+     AY  VV  L E+G+MVI DNH+S+P+WCC++DDGNGFFGDRYF+P 
Sbjct: 122 EHNNPELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPN 181

Query: 122 EWLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIV 181
            W++GL L+A  F+  P VV MSLRNE+RG       W+ ++  G  T+H  NP VLVI+
Sbjct: 182 TWVEGLGLMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVIL 241

Query: 182 SGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFV 241
           SGL FD DL  L   P+ +     K+V+E H YSF     +       N++C N    F 
Sbjct: 242 SGLQFDTDLSFLPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGL----PNDVCKNETGRFK 301

Query: 242 DHAEFVREGPN--PFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSY 301
            +  FV    N    PLF+SE+G DQR VN  +NR+++C  A+LA+EDLDWALWT  GSY
Sbjct: 302 SNVGFVTSSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSY 361

Query: 302 YYREGQTGP---AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPN-SNASFSYVIYHPQ 361
           YYR  +       ET+G  + +W++I+NP+F+ + + +Q  +QDP  +   +  +IYHP 
Sbjct: 362 YYRSDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPA 421

Query: 362 SGQCIQVSNDNKDIFMGNC-SISSRWTHD-NDSTPIRMSSTGLCLKTSGEGLMPSLSTDC 421
           SG C++ S     + +G+C S+ SRW +D +   PI +  +  C+ T G GL   ++ +C
Sbjct: 422 SGLCVE-SGIGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENC 481

Query: 422 FGPQSS-WTAISNTKLHLAT--VTQDGKSLCLQVDVDFSPPLLSN 454
             P ++ W+ +S+ +L L T  + +DGK   + +D   SP + +N
Sbjct: 482 SAPNNTLWSTVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTN 520

BLAST of CmoCh09G011940 vs. ExPASy Swiss-Prot
Match: P54583 (Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0614 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.7e-13
Identity = 79/339 (23.30%), Postives = 138/339 (40.71%), Query Frame = 0

Query: 2   LIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQ 61
           ++ GL  R  + + D+   L +N +RL Y+  +       T+  + +   + Q   GL  
Sbjct: 78  VVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDIL---KPGTMPNSINFYQMNQDLQGLTS 137

Query: 62  YNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQEW 121
               V++K +A A        G+ GL +I D H    R  CS    +  +         W
Sbjct: 138 LQ--VMDKIVAYA--------GQIGLRIILDRH----RPDCS--GQSALWYTSSVSEATW 197

Query: 122 LQGLSLVAQRFSKKPTVVGMSLRNEIR-----GTNENANDWNNYVTQGVTTIHNINPNVL 181
           +  L  +AQR+   PTVVG  L NE       G  + + DW     +    + ++NPN+L
Sbjct: 198 ISDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLL 257

Query: 182 VIVSGLNFDN--------DLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLN 241
           + V G+   N        +L+   + P+ V  + N+LV+  H Y+ S   ++ F +    
Sbjct: 258 IFVEGVQSYNGDSYWWGGNLQGAGQYPV-VLNVPNRLVYSAHDYATSVYPQTWFSDPTFP 317

Query: 242 NICANIIN---GFVDHAEFVREGPNPFPLFVSEYGYD-QREVNHAENRFMSCF---TAHL 301
           N    I N   G++ +        N  P+++ E+G   Q   +    + +  +   TA  
Sbjct: 318 NNMPGIWNKNWGYLFN-------QNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTAQY 377

Query: 302 AQEDLDWALWTWQGSYYYREGQTGPAETFGVLDSNWTQI 321
             +   W  W+W        G TG     G+L  +W  +
Sbjct: 378 GADSFQWTFWSWNPD----SGDTG-----GILKDDWQTV 380

BLAST of CmoCh09G011940 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 3.6e-13
Identity = 72/341 (21.11%), Postives = 135/341 (39.59%), Query Frame = 0

Query: 3   IEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQY 62
           + GL  R +  + D+  K  +N +RL Y+ Q+F   +        D +D  +        
Sbjct: 71  LHGLWSRSMDDMLDQVKKEGYNLIRLPYSNQLFDSSSRP------DSIDYHK-------- 130

Query: 63  NPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQ--- 122
           NP ++     +  + +++  G+ G+ +I D H            G+G   + ++  Q   
Sbjct: 131 NPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHR----------PGSGGQSELWYTSQYPE 190

Query: 123 -EWLQGLSLVAQRFSKKPTVVGMSLRNEIR-----GTNENANDWNNYVTQGVTTIHNINP 182
             W+    ++A R+   PTV+G  L NE       GT   + DW     +    I ++NP
Sbjct: 191 SRWISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQRAGNAILSVNP 250

Query: 183 NVLVIVSGLN-----------FDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKF 242
           N L++V G++           +  +L  +   P+ V  + N++V+  H Y   G     +
Sbjct: 251 NWLILVEGVDHNVQGNNSQYWWGGNLTGVANYPV-VLDVPNRVVYSPHDYG-PGVSSQPW 310

Query: 243 INQPLNNICANIINGFVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLA 302
            N P      + +    D         N  P+ V E+G    +++  E ++ +    ++ 
Sbjct: 311 FNDP---AFPSNLPAIWDQTWGYISKQNIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIG 370

Query: 303 QEDLDWALWTWQGSYYYREGQTGPAETFGVLDSNWTQIKNP 324
             +L +  W    S     G TG     G+L  +WT    P
Sbjct: 371 ANNLYFTYW----SLNPNSGDTG-----GLLLDDWTTWNRP 373

BLAST of CmoCh09G011940 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 1.3e-10
Identity = 85/356 (23.88%), Postives = 138/356 (38.76%), Query Frame = 0

Query: 2   LIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMF---TRYANRTVEENFDLLDLEQAKAG 61
           ++ GL  R  K +  +   L FN VRL +        T  A+     N DL  L   +  
Sbjct: 58  VMHGLWARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ-- 117

Query: 62  LAQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDP 121
                  +L+K IAE            G+ V+ D+H      C  + +   ++   Y + 
Sbjct: 118 -------ILDKVIAE--------FNARGMYVLLDHHTPD---CAGISE--LWYTGSYTEA 177

Query: 122 QEWLQGLSLVAQRFSKKPTVVGMSLRNEIR-----GTNENANDWNNYVTQGVTTIHNINP 181
           Q WL  L  VA R+   P V+G+ L+NE       GT   A DWN    +G   +  + P
Sbjct: 178 Q-WLADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAP 237

Query: 182 NVLVIVSGLN------------FDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESK 241
             L+ V G+             +  +L+ L   PLN+    N+L+   H+Y      +S 
Sbjct: 238 KWLIAVEGITDNPVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVYGPDVFVQSY 297

Query: 242 FINQPL-NNICANIINGFVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAH 301
           F +    NN+ A     F   A           L + E+G    E +  +  +      +
Sbjct: 298 FNDSNFPNNMPAIWERHFGQFA-------GTHALLLGEFGGKYGEGDARDKTWQDALVKY 357

Query: 302 LAQEDLDWAL-WTWQGSYYYREGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTM 336
           L  + ++    W+W  +     G TG     G+L  +WT ++      K  LL+T+
Sbjct: 358 LRSKGINQGFYWSWNPN----SGDTG-----GILRDDWTSVRQ----DKMTLLRTL 368

BLAST of CmoCh09G011940 vs. ExPASy TrEMBL
Match: A0A6J1EXF1 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111439273 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.3e-265
Identity = 444/453 (98.01%), Postives = 448/453 (98.90%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. ExPASy TrEMBL
Match: A0A6J1F4E4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111440529 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 4.1e-262
Identity = 440/453 (97.13%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 54  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 113

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 114 QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 173

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 174 WLQGLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 233

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 234 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 293

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 294 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 353

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 354 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 413

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 414 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 473

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 474 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 506

BLAST of CmoCh09G011940 vs. ExPASy TrEMBL
Match: A0A6J1EXF6 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111439277 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 4.1e-262
Identity = 440/453 (97.13%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. ExPASy TrEMBL
Match: A0A6J1EPM0 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111436676 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.9e-260
Identity = 437/453 (96.47%), Postives = 441/453 (97.35%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLS VAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSFVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
            LNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 SLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLS DCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSKDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. ExPASy TrEMBL
Match: A0A6J1IC06 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111474044 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.6e-258
Identity = 432/453 (95.36%), Postives = 439/453 (96.91%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 72  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 131

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 132 QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 191

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 192 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 251

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLN S LDNKLVFEVHLYSFSGD ESKFINQPLNNICA+IINGFVD
Sbjct: 252 GLNFDNDLRCLKEKPLNASALDNKLVFEVHLYSFSGDAESKFINQPLNNICADIINGFVD 311

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 312 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 371

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 372 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 431

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKD+FMGNCS S RWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSW A
Sbjct: 432 SNDNKDMFMGNCSNSGRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWRA 491

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLAT+TQDGKSLCLQV+   S  +++N
Sbjct: 492 ISNTKLHLATITQDGKSLCLQVENSNSSKIVTN 524

BLAST of CmoCh09G011940 vs. NCBI nr
Match: XP_022932811.1 (uncharacterized protein LOC111439273 [Cucurbita moschata])

HSP 1 Score: 924.5 bits (2388), Expect = 4.8e-265
Identity = 444/453 (98.01%), Postives = 448/453 (98.90%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. NCBI nr
Match: XP_022933313.1 (uncharacterized protein LOC111440529 [Cucurbita moschata])

HSP 1 Score: 913.7 bits (2360), Expect = 8.4e-262
Identity = 440/453 (97.13%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 54  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 113

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 114 QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 173

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 174 WLQGLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 233

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 234 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 293

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 294 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 353

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 354 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 413

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 414 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 473

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 474 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 506

BLAST of CmoCh09G011940 vs. NCBI nr
Match: XP_022932816.1 (uncharacterized protein LOC111439277 [Cucurbita moschata])

HSP 1 Score: 913.7 bits (2360), Expect = 8.4e-262
Identity = 440/453 (97.13%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. NCBI nr
Match: XP_022930167.1 (uncharacterized protein LOC111436676 [Cucurbita moschata])

HSP 1 Score: 907.5 bits (2344), Expect = 6.0e-260
Identity = 437/453 (96.47%), Postives = 441/453 (97.35%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLS VAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 121 WLQGLSFVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
            LNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD
Sbjct: 181 SLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 301 EGQAGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLS DCFGPQSSWTA
Sbjct: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSKDCFGPQSSWTA 420

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLATVTQDGKSLCLQV+   S  +++N
Sbjct: 421 ISNTKLHLATVTQDGKSLCLQVESSNSSKIVTN 453

BLAST of CmoCh09G011940 vs. NCBI nr
Match: KAG6592274.1 (hypothetical protein SDJN03_14620, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907.5 bits (2344), Expect = 6.0e-260
Identity = 436/453 (96.25%), Postives = 442/453 (97.57%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 60
           MLIEGLNHRPLK LADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA
Sbjct: 75  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLA 134

Query: 61  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 120
           QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE
Sbjct: 135 QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 194

Query: 121 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 180
           WLQGLSLVAQRFSKK TVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS
Sbjct: 195 WLQGLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 254

Query: 181 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 240
           GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANII+GFVD
Sbjct: 255 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIIDGFVD 314

Query: 241 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 300
           HAEFVREGPNPFPLFVSEYGYDQREVN AENRFMSCFTAHLAQEDLDWALWTWQGSYYYR
Sbjct: 315 HAEFVREGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 374

Query: 301 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQ GP ETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV
Sbjct: 375 EGQAGPVETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 434

Query: 361 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWTA 420
           SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSW A
Sbjct: 435 SNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGPQSSWRA 494

Query: 421 ISNTKLHLATVTQDGKSLCLQVDVDFSPPLLSN 454
           ISNTKLHLAT+TQDGKSLCLQV+   S  +++N
Sbjct: 495 ISNTKLHLATITQDGKSLCLQVESSNSSKIVTN 527

BLAST of CmoCh09G011940 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 359.4 bits (921), Expect = 5.7e-99
Identity = 183/448 (40.85%), Postives = 272/448 (60.71%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYA---NRTVEENFDLLDLEQAKA 60
           ++ EGL+ +P+  +A + +++ FNCVRLT+   + T      N TV ++F  L L     
Sbjct: 64  VVAEGLSKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIV 123

Query: 61  GLAQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFD 120
           G    NP +++  + EAY+ VV  LG + +MVI DNH+++P WCC+ DDGNGFFGD++FD
Sbjct: 124 GFQTNNPSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFD 183

Query: 121 PQEWLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLV 180
           P  W+  L  +A  F+    VVGMSLRNE+RG  +N NDW  Y+ QG   +H+ N  VLV
Sbjct: 184 PTVWVAALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLV 243

Query: 181 IVSGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIING 240
           I+SGL+FD DL  ++ +P+ +S    KLVFE+H YSFS D  S   N P N+IC  ++N 
Sbjct: 244 ILSGLSFDADLSFVRSRPVKLS-FTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNR 303

Query: 241 FVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSY 300
             +   ++      FPLF+SE+G D+R VN  +NR+  C T   A+ D+DW+LW   GSY
Sbjct: 304 IGNGGGYLLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSY 363

Query: 301 YYREGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y R+G+ G  E +GVLDS+W  ++N +F+QK   LQ+ LQ P        +++HP +G C
Sbjct: 364 YLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLC 423

Query: 361 IQVS-NDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSG-EGLMPSLSTDCFGPQ 420
           I  S +D K + +G C+ S  W++   +  +R+    LCL+++G +  +    T C    
Sbjct: 424 IVRSLDDPKMLTLGPCNSSEPWSYTKKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSG 483

Query: 421 SSWTAISNTKLHLATVTQDGKSLCLQVD 444
           S W  IS +++HLA+ T +  SLCL VD
Sbjct: 484 SKWQTISASRMHLASTTSNKTSLCLDVD 504

BLAST of CmoCh09G011940 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.7e-90
Identity = 172/449 (38.31%), Postives = 265/449 (59.02%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTRYA---NRTVEENFDLLDLEQAKA 60
           ++ EGL+ + +  LA + + + FNCVR T+   + T      N TV ++F  L L    +
Sbjct: 34  VVAEGLSKQSVDDLAKKIMAMGFNCVRFTWPLDLATNETLANNVTVRQSFQSLGLNDDIS 93

Query: 61  GLAQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFD 120
           G    NP +++  + EAY+ VV  LG + +MVI DNH+++P WCC  +DGNGFFGD +FD
Sbjct: 94  GFETKNPSMIDLPLIEAYKKVVAKLGNNNVMVILDNHVTKPGWCCGYNDGNGFFGDTFFD 153

Query: 121 PQEWLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLV 180
           P  W+ GL+ +A  F     VVGMSLRNE+RG  +N +DW  Y+ QG   +H  NPNVLV
Sbjct: 154 PTTWIAGLTKIAMTFKGATNVVGMSLRNELRGPKQNVDDWFKYMQQGAEAVHEANPNVLV 213

Query: 181 IVSGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIING 240
           I+SGL++D DL  ++ + +N+ T   KLVFE+H YSF+    + + ++  N  C  I+  
Sbjct: 214 ILSGLSYDTDLSFVRSRHVNL-TFTRKLVFELHRYSFT--NTNTWSSKNPNEACGEILKS 273

Query: 241 FVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSY 300
             +   F       FP+F+SE+G D R  N  +NR++ C     A+ D+DW++WT QGSY
Sbjct: 274 IENGGGF---NLRDFPVFLSEFGIDLRGKNVNDNRYIGCILGWAAENDVDWSIWTLQGSY 333

Query: 301 YYREGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y REG  G +E +G+LDS+W ++++ +F+Q+  L+ + LQ P S +    +++HP +G C
Sbjct: 334 YLREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLILSPLQGPGSQSKVYNLVFHPLTGLC 393

Query: 361 -IQVSNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLS-TDCFGPQ 420
            +Q   D   + +G C+ S  W++   +T + +    LCL+++G      LS T C  P 
Sbjct: 394 MLQSILDPTKVTLGLCNESQPWSYTPQNT-LTLKDKSLCLESTGPNAPVKLSETSCSSPN 453

Query: 421 -SSWTAISNTKLHLATVTQDGKSLCLQVD 444
            S W  IS + + LA  + +  SLCL VD
Sbjct: 454 LSEWETISASNMLLAAKSTN-NSLCLDVD 474

BLAST of CmoCh09G011940 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 324.3 bits (830), Expect = 2.0e-88
Identity = 180/454 (39.65%), Postives = 259/454 (57.05%), Query Frame = 0

Query: 4   EGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTR---YANRTVEENFDLLDLEQAKAGLA 63
           EGL+ +PL  +A++ + + FNCVRLT+   + T     A  TV ++     L +A +G  
Sbjct: 57  EGLSKQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQ 116

Query: 64  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 123
            +NP +L+  + +A++ VV  L +  +MVI DNH+SQP WCCS +DGNGFFGD++ +PQ 
Sbjct: 117 THNPTILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQV 176

Query: 124 WLQGLSLVAQRFSK-KPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIV 183
           W++GL  +A  F+     VVGMSLRNE+RG  +N  DW  Y+ +G   +H++NPNVLVIV
Sbjct: 177 WIKGLKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIV 236

Query: 184 SGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFV 243
           SGLN+  DL  L+E+P  VS    K+VFE+H Y F    E       LN IC       +
Sbjct: 237 SGLNYATDLSFLRERPFEVS-FRRKVVFEIHWYGFWNTWE----GDNLNKICGKETEKMM 296

Query: 244 DHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYY 303
             + F+ E     PLFVSE+G DQR  N  +N+F+SCF A  A  DLDW+LWT  GSYY 
Sbjct: 297 KMSGFLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYI 356

Query: 304 REGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQT-----MLQDPNSNASFSYVIYHPQS 363
           RE   G  E++GVLD NW+ I+N   +Q    +QT     M   P        +++HP +
Sbjct: 357 REKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPST 416

Query: 364 GQCIQVSNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCFGP 423
           G CI V      + +G+C+ S  W   +           LCLK   +G   S+    F  
Sbjct: 417 GLCI-VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKG--KSVKLRLFFS 476

Query: 424 Q---SSWTAISNTKLHLATVTQDGKSLCLQVDVD 446
           +   S W   S++K+ L+++T++G S+CL VD +
Sbjct: 477 ESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTE 494

BLAST of CmoCh09G011940 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 4.4e-83
Identity = 165/451 (36.59%), Postives = 254/451 (56.32%), Query Frame = 0

Query: 1   MLIEGLNHRPLKVLADEAIKLRFNCVRLTYATQMF---TRYANRTVEENFDLLDLEQAKA 60
           ++ EGL+ +P+  ++ +   + FNCVRLT+  ++    T   N TV+++F+   L+    
Sbjct: 57  VVAEGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQ 116

Query: 61  GLAQYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFD 120
           G+  +NP+++N  +   ++AVV  LG   +MVI DNH + P WCCS DD + FFGD  F+
Sbjct: 117 GIYTHNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFN 176

Query: 121 PQEWLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLV 180
           P  W+ GL  +A  F     VVGMSLRNE+RG N  + DW  Y+ +G   +H  NPNVLV
Sbjct: 177 PDLWMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLV 236

Query: 181 IVSGLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIING 240
           I+SGLNFD DL  LK++P+N+S    KLV E+H YSF+ D   ++ +  +N+ C+ + + 
Sbjct: 237 ILSGLNFDADLSFLKDRPVNLS-FKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSK 296

Query: 241 FVDHAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSY 300
                 FV +    FPLF+SE+G DQR  +   NR+M+C  A  A++DLDWA+W   G Y
Sbjct: 297 ERRTGGFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVY 356

Query: 301 YYREGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y+REG+ G  E +G+LD+NW  + N  ++++  ++Q     P    +    I+HP +G C
Sbjct: 357 YFREGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLC 416

Query: 361 IQVSN--DNKDIFMGNCSISSRWTHDNDS-TPIRMSSTGLCLKTSGEGLMPSLSTDCFGP 420
           +   +     ++ +G C+    W++ +     IR          +  G    L   C   
Sbjct: 417 LVRKSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRIC--- 476

Query: 421 QSSWTAISNTKLHLATVTQDGKSLCLQVDVD 446
            +    IS TK+HL+  T DG  +CL VD D
Sbjct: 477 -TKIEQISATKMHLSFNTSDGSLVCLDVDSD 499

BLAST of CmoCh09G011940 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 4.4e-67
Identity = 155/448 (34.60%), Postives = 237/448 (52.90%), Query Frame = 0

Query: 4   EGLNHRPLKVLADEAIKLRFNCVRLTYATQMFTR---YANRTVEENFDLLDLEQAKAGLA 63
           EGL+ +PL  ++ + + + FNCVRLT+   + T        TV+++F+ L L +   G+ 
Sbjct: 58  EGLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQ 117

Query: 64  QYNPFVLNKTIAEAYEAVVDVLGESGLMVIADNHMSQPRWCCSLDDGNGFFGDRYFDPQE 123
            +NP +L+  +  A++ VV  LGE+G+MVI DNH++ P WCC  +D + FFG  +FDP  
Sbjct: 118 THNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLV 177

Query: 124 WLQGLSLVAQRFSKKPTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVS 183
           W +GL  +A  F     V+GMSLRNE RG  +  + W  ++ QG   +H  NP +LVI+S
Sbjct: 178 WAKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILS 237

Query: 184 GLNFDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDQESKFINQPLNNICANIINGFVD 243
           G++FD +L  L+++ +NVS  D KLVFE+H YSFS  ++S +     N+ C  II     
Sbjct: 238 GIDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFSDGRDS-WRKHNSNDFCVKIIEKVTH 297

Query: 244 HAEFVREGPNPFPLFVSEYGYDQREVNHAENRFMSCFTAHLAQEDLDWALWTWQGSYYYR 303
           +  F+      FPL +SE+G DQR  + + NR+M+C  A  A+ DLDWA+W   G YY R
Sbjct: 298 NGGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLR 357

Query: 304 EGQTGPAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI-- 363
              TGP                                PN N     +++HP +G C+  
Sbjct: 358 ---TGPG-----------------------------LRPNKN-----LLFHPSTGLCVTN 417

Query: 364 QVSNDNKDIFMGNCSISSRWTHDNDSTPIRMSSTGLCLKTS---GEGLMPSLSTDCFGPQ 423
             S++   + +G C  S  WT +     + ++   +C++     G+ +   + T C    
Sbjct: 418 NPSDNIPTLRLGPCPKSDPWTFNPSEGILWINK--MCVEAPNVVGQKVKLGVGTKC---- 458

Query: 424 SSWTAISNTKLHLATVTQDGKSLCLQVD 444
           S    IS TK+HL+  T +G  LCL VD
Sbjct: 478 SKLGQISATKMHLSFKTSNGLLLCLDVD 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA09.5e-9941.29Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P545832.7e-1323.30Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351... [more]
P235483.6e-1321.11Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
P194871.3e-1023.88Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
A0A6J1EXF12.3e-26598.01Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111439273 P... [more]
A0A6J1F4E44.1e-26297.13Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111440529 P... [more]
A0A6J1EXF64.1e-26297.13Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111439277 P... [more]
A0A6J1EPM02.9e-26096.47Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111436676 P... [more]
A0A6J1IC063.6e-25895.36Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111474044 PE=... [more]
Match NameE-valueIdentityDescription
XP_022932811.14.8e-26598.01uncharacterized protein LOC111439273 [Cucurbita moschata][more]
XP_022933313.18.4e-26297.13uncharacterized protein LOC111440529 [Cucurbita moschata][more]
XP_022932816.18.4e-26297.13uncharacterized protein LOC111439277 [Cucurbita moschata][more]
XP_022930167.16.0e-26096.47uncharacterized protein LOC111436676 [Cucurbita moschata][more]
KAG6592274.16.0e-26096.25hypothetical protein SDJN03_14620, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT1G13130.15.7e-9940.85Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.7e-9038.31Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.12.0e-8839.65Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.14.4e-8336.59Glycosyl hydrolase superfamily protein [more]
AT5G16700.14.4e-6734.60Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 11..294
e-value: 3.4E-25
score: 89.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..336
e-value: 5.4E-59
score: 202.1
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 1..444
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 3..330
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 343..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh09G011940.1CmoCh09G011940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds