CmoCh09G009770 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh09G009770
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	MBOAT (membrane bound O-acyl transferase) family protein
Location	Cmo_Chr09: 5174793 .. 5175746 (-)
RNA-Seq Expression	CmoCh09G009770
Synteny	CmoCh09G009770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTGATACTCATATCAATGACCTCTCTATTGTATTGCTATTTCATAGCTCCAAAACTTCCCAAAGGCAAATTCAGGCTCATTTCGTTCCTCCCCGTCTTCTCCCTCTTCCTCGTCCTCCCTCTCTATGTCTCGTCCGCCTTACTCTCGAGTAGCTTTGCCTTCTTCATCACCTGGCTCACGACCTTTAAGCTCGTTCTATTCTCATTCAATTTAGGTCCTTTAGCTTTCGACTCGAAAGTATCGTTCACTTTGTTTGCTTCCATTGCGTTCTTACCCACTAGAATCAAGCATAACAAAACAGCCAAATACGTTCAAAATCATGACGATCTTGGCAACGATCCACACCCTAAGCTGCCTCTAAGCTTGCCAGCCAAGGTGGTGATATTTGCTATGTTGGTCATGGGTAAGAGGCACTTAGAAATTATGGATCCCATTGCAAATTTGTACGTGAATTGTGCAACGTTGTATTTCTATTTAGACATGACTATGAGTCTTTCTAATGTGTTTGTTCGATCGAGATTTGGCGTTGAGGTTAGACAACCGTTTGACGAGCCATATCTCGCGACGTCGCTTCAAAATTTTTGGGGACGGAGATGGAATCGATCTGTGTCGGAAACTTTGCATACCGCTGTTTATCAACCCGTTCGTTATGAAATCGGAGTGCCACGGTGGATGGCGGTGGTCATTGTGTTTGTGGTGTCCGGGTTGATGCATGAGCTTTTGCTTTACTATATCATTAGAGTGATCCCAACGTGGGAAGTTACATGGTTCTTTGTTGTTCATGGACTGTGTGTGGCTGTGGAGATTGAGCTGAAAAGGGCTTTGGGGAAAAGGTGGCGGATTCATTCGGCGGTGTCCCGACCACTCACGATAGCGTTTTTCGTAGCCACCGTTCATTGGTTGTTTTTTCCACCACTGTTGAGGATAAAACACTTCCCAAGTGGCTAG

mRNA sequence

ATGAAGGTGATACTCATATCAATGACCTCTCTATTGTATTGCTATTTCATAGCTCCAAAACTTCCCAAAGGCAAATTCAGGCTCATTTCGTTCCTCCCCGTCTTCTCCCTCTTCCTCGTCCTCCCTCTCTATGTCTCGTCCGCCTTACTCTCGAGTAGCTTTGCCTTCTTCATCACCTGGCTCACGACCTTTAAGCTCGTTCTATTCTCATTCAATTTAGGTCCTTTAGCTTTCGACTCGAAAGTATCGTTCACTTTGTTTGCTTCCATTGCGTTCTTACCCACTAGAATCAAGCATAACAAAACAGCCAAATACGTTCAAAATCATGACGATCTTGGCAACGATCCACACCCTAAGCTGCCTCTAAGCTTGCCAGCCAAGGTGGTGATATTTGCTATGTTGGTCATGGGTAAGAGGCACTTAGAAATTATGGATCCCATTGCAAATTTGTACGTGAATTGTGCAACGTTGTATTTCTATTTAGACATGACTATGAGTCTTTCTAATGTGTTTGTTCGATCGAGATTTGGCGTTGAGGTTAGACAACCGTTTGACGAGCCATATCTCGCGACGTCGCTTCAAAATTTTTGGGGACGGAGATGGAATCGATCTGTGTCGGAAACTTTGCATACCGCTGTTTATCAACCCGTTCGTTATGAAATCGGAGTGCCACGGTGGATGGCGGTGGTCATTGTGTTTGTGGTGTCCGGGTTGATGCATGAGCTTTTGCTTTACTATATCATTAGAGTGATCCCAACGTGGGAAGTTACATGGTTCTTTGTTGTTCATGGACTGTGTGTGGCTGTGGAGATTGAGCTGAAAAGGGCTTTGGGGAAAAGGTGGCGGATTCATTCGGCGGTGTCCCGACCACTCACGATAGCGTTTTTCGTAGCCACCGTTCATTGGTTGTTTTTTCCACCACTGTTGAGGATAAAACACTTCCCAAGTGGCTAG

Coding sequence (CDS)

ATGAAGGTGATACTCATATCAATGACCTCTCTATTGTATTGCTATTTCATAGCTCCAAAACTTCCCAAAGGCAAATTCAGGCTCATTTCGTTCCTCCCCGTCTTCTCCCTCTTCCTCGTCCTCCCTCTCTATGTCTCGTCCGCCTTACTCTCGAGTAGCTTTGCCTTCTTCATCACCTGGCTCACGACCTTTAAGCTCGTTCTATTCTCATTCAATTTAGGTCCTTTAGCTTTCGACTCGAAAGTATCGTTCACTTTGTTTGCTTCCATTGCGTTCTTACCCACTAGAATCAAGCATAACAAAACAGCCAAATACGTTCAAAATCATGACGATCTTGGCAACGATCCACACCCTAAGCTGCCTCTAAGCTTGCCAGCCAAGGTGGTGATATTTGCTATGTTGGTCATGGGTAAGAGGCACTTAGAAATTATGGATCCCATTGCAAATTTGTACGTGAATTGTGCAACGTTGTATTTCTATTTAGACATGACTATGAGTCTTTCTAATGTGTTTGTTCGATCGAGATTTGGCGTTGAGGTTAGACAACCGTTTGACGAGCCATATCTCGCGACGTCGCTTCAAAATTTTTGGGGACGGAGATGGAATCGATCTGTGTCGGAAACTTTGCATACCGCTGTTTATCAACCCGTTCGTTATGAAATCGGAGTGCCACGGTGGATGGCGGTGGTCATTGTGTTTGTGGTGTCCGGGTTGATGCATGAGCTTTTGCTTTACTATATCATTAGAGTGATCCCAACGTGGGAAGTTACATGGTTCTTTGTTGTTCATGGACTGTGTGTGGCTGTGGAGATTGAGCTGAAAAGGGCTTTGGGGAAAAGGTGGCGGATTCATTCGGCGGTGTCCCGACCACTCACGATAGCGTTTTTCGTAGCCACCGTTCATTGGTTGTTTTTTCCACCACTGTTGAGGATAAAACACTTCCCAAGTGGCTAG

Protein sequence

MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITWLTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKLPLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEVRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATVHWLFFPPLLRIKHFPSG

Homology

[Close]

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 128/318 (40.25%), Postives = 184/318 (57.86%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +KV + ++ S+ YCY+I P++  G  R +S  PV +LFLVLPL+ SS  LS   AFF+TW
Sbjct: 9   IKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITAFFLTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL      +F  F      P +++ N  +   QNH        PKL
Sbjct: 69  LANFKLILFSFDKGPL-IPIPTNFPRFFCFTCFPIKVQQNPKS---QNH-------LPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
             ++  K+ IFA+L+    + + + P   L +    LY  +++ ++   V V    G ++
Sbjct: 129 VFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDL 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVR------YEIGVPRWMAVVIVFV 240
               ++PYLATSLQ+FWGRRWN  V   L  AVY P+R         G   +  ++  F+
Sbjct: 189 EPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFI 248

Query: 241 VSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKR--ALGKRWRIHSAVSRPLT 300
           VSGL+HELL +Y+IR +PT EVT FFV+HG+C AVE+ +K+   + +RWR+   VSR LT
Sbjct: 249 VSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLT 308

Query: 301 IAFFVATVHWLFFPPLLR 311
           + F   T  WLF P L R
Sbjct: 309 VGFVFVTGGWLFTPQLKR 313

[Close]

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 120/315 (38.10%), Postives = 176/315 (55.87%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +K+ + ++ S+ YCY+++  +  G FRL+S LPV +LFLV PL+ S    S   AFF++W
Sbjct: 9   IKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMAFFLSW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL+     + + F  I   P + + N     +QN+         K+
Sbjct: 69  LANFKLILFSFDQGPLS-PLPRTLSRFICITCFPIKPQQNPN---IQNY---------KI 128

Query: 121 PLSLPA-KVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVE 180
           P+ L A KVVIF +L+    + + + P   L  N   ++  L++   L    V    G +
Sbjct: 129 PIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCD 188

Query: 181 VRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVR------YEIGVPRWMAVVIVF 240
           +    +EPYLATSLQ+FWGRRWN  V   L  AVY P R             ++ V   F
Sbjct: 189 LEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAF 248

Query: 241 VVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKR--WRIHSAVSRPL 300
           +VSG +HE+L +Y+ R +PT EVTWFF++HG+C   E+ +K++   R  WR+   VSR L
Sbjct: 249 LVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLL 308

Query: 301 TIAFFVATVHWLFFP 307
           T+ F V T  W FFP
Sbjct: 309 TVGFVVVTSGWFFFP 310

[Close]

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.8e-49
Identity = 123/318 (38.68%), Postives = 175/318 (55.03%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +KV + ++ S+ YCY+ + ++  G FRL+S LPV  LFLVLPL+VSS   S S AFF++W
Sbjct: 9   IKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTAFFLSW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL F    + + F      P +++ N               P  ++
Sbjct: 69  LANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQNP-------------KPQNQM 128

Query: 121 P-LSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVE 180
           P      K+  F +L+    + + M P   L +    +Y   ++ ++   V +      +
Sbjct: 129 PKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 188

Query: 181 VRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIG------VPRWMAVVIVF 240
           +   F+EPYL+TSLQ+FWGRRWN  V   L  AVY PVR   G         ++ V   F
Sbjct: 189 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 248

Query: 241 VVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRA-LGKRWRIHSAVSRPLT 300
           +VSG++HEL+ +Y  R  PT EVT FFV+HG+C A E   KR  L +RW++   VSR LT
Sbjct: 249 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLT 308

Query: 301 IAFFVATVHWLFFPPLLR 311
           + F V T  WLFFP L R
Sbjct: 309 VGFVVMTGGWLFFPHLAR 312

[Close]

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-48
Identity = 120/318 (37.74%), Postives = 183/318 (57.55%), Query Frame = 0


Query: 2   KVILISMTSLLYCYFIAPKLPK-GKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 61
           +V + ++ +  YC F+    P  G  RL+  LPV  LF+ LPL +SS  L    A ++ W
Sbjct: 12  EVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPTALYLVW 71

Query: 62  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 121
           L  FKL+LF+F+LGPL+ +  +S   F S   LP + + + +  + +N   L  +   K+
Sbjct: 72  LANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLSFE-WRKV 131

Query: 122 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 181
            L + AK+V FA ++      + +       + C   Y   ++T++ S V  R+  G+++
Sbjct: 132 VLFV-AKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARATLGLDL 191

Query: 182 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWM--------AVVIV 241
              F+EPYLATSLQ+FWGRRWN  VS+ L    YQPVR  +   RW+        A+++ 
Sbjct: 192 YPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLS--RWVRLRWEVAGAMLVA 251

Query: 242 FVVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLT 301
           F VSGLMHE+  +Y+ R  P+WEVT FFV+HG+C AVE+ +K+A+  + R+   VS  LT
Sbjct: 252 FTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSGALT 311

Query: 302 IAFFVATVHWLFFPPLLR 311
           + F + T  WLF P L+R
Sbjct: 312 VGFVMVTGGWLFLPQLVR 324

[Close]

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.9e-48
Identity = 112/307 (36.48%), Postives = 172/307 (56.03%), Query Frame = 0


Query: 10  SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITWLTTFKLVLF 69
           SL Y +FIA  +PKG  RLI F PVF +F ++P  + S  L    AFFI WL  FKL+LF
Sbjct: 14  SLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWLANFKLLLF 73

Query: 70  SFNLGPLAFDSK-VSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKLPLSLPAKV 129
           +   GPL+ + K +S  +F +++ LP +I+ +       +H+        + PL    K 
Sbjct: 74  ALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEG-----STEGPLIYTIKA 133

Query: 130 VIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEVRQPFDEPY 189
           V   +++    +   +     L +    +YF L++ ++ +   VR+   +E+   F++PY
Sbjct: 134 VFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPY 193

Query: 190 LATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGV--PRW---MAVVIVFVVSGLMHELL 249
           LATSLQ+FWGRRWN  V+  L   VY+P      V  P +   +A    FVVSG+MHEL+
Sbjct: 194 LATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFVVSGIMHELI 253

Query: 250 LYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATVHWL 309
            +Y+ R+ P W++ WFF+++G C  VEI +K+ +  RWR   A+S+ LT+ F + T  WL
Sbjct: 254 FFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALWL 313

Query: 310 FFPPLLR 311
           F P   R
Sbjct: 314 FLPEFNR 315

[Close]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL
Match: A0A6J1FFF7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3662 GN=LOC111443462 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW
Sbjct: 9   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV
Sbjct: 129 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
           RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH
Sbjct: 189 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV
Sbjct: 249 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 308

Query: 301 HWLFFPPLLRIKHFPSG 318
           HWLFFPPLLRIKHFPSG
Sbjct: 309 HWLFFPPLLRIKHFPSG 325

[Close]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL
Match: A0A6J1ILW9 (probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111476789 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.5e-159
Identity = 284/317 (89.59%), Postives = 298/317 (94.01%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKV+LISM SLLYCYFIA KL KGKFRLISFLPVFSLFLVLPLYVSSA LSS+ +FF+TW
Sbjct: 9   MKVLLISMVSLLYCYFIAQKLSKGKFRLISFLPVFSLFLVLPLYVSSAFLSSNISFFVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKL+LFSFNLGPLAFD K+S +LF SIAFLPTRIKHNKTAKYVQNHDDLGN+P PKL
Sbjct: 69  LTTFKLILFSFNLGPLAFDPKLSLSLFVSIAFLPTRIKHNKTAKYVQNHDDLGNEPRPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PLSLPAKVVIFAMLVMG RHLEI  PIA LY+NCATLYFYLDM MSLSNVFVRSRFGVEV
Sbjct: 129 PLSLPAKVVIFAMLVMGGRHLEIGGPIAKLYLNCATLYFYLDMIMSLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
           R+PFDEPYLATSLQNFWGRRWN+SVSETLHTAVYQPVRYEIGVPRWMAVV+VFVVSGLMH
Sbjct: 189 RRPFDEPYLATSLQNFWGRRWNQSVSETLHTAVYQPVRYEIGVPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIR IPTWE+TWFFV HGLCVAVEIELKRALGKRWRIH AVSRPLTIAF VAT+
Sbjct: 249 ELLLYYIIRAIPTWEITWFFVAHGLCVAVEIELKRALGKRWRIHWAVSRPLTIAFLVATI 308

Query: 301 HWLFFPPLLRIKHFPSG 318
           HWLFFPPLLRIKH PSG
Sbjct: 309 HWLFFPPLLRIKHSPSG 325

[Close]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL
Match: A0A6J1FEY7 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443337 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.4e-150
Identity = 268/310 (86.45%), Postives = 287/310 (92.58%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSSSFAF +TW
Sbjct: 9   MKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSSFAFLVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSK+SF LFASIAFLPTRIKH+KTAKY QN +++G +P PK+
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYTQNQEEVGKEPRPKI 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PL+LPAKVVIF ML+MGK++ EI+DPIA L +NCATLYFYLDM M LSNVFVRSRFGVEV
Sbjct: 129 PLNLPAKVVIFVMLIMGKKYYEIVDPIAKLCMNCATLYFYLDMIMCLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
            +PFDEPYLATSLQNFWGRRWNR VSETLH AVYQPVRYE+G PRWMAVV+VFVVSGLMH
Sbjct: 189 SRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIR IPTWEVTWFFV+HGLCVAVEIELKRALGKRWRIH AVS PLT+AF VAT 
Sbjct: 249 ELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPLTMAFLVATA 308

Query: 301 HWLFFPPLLR 311
           HWLFFPPLLR
Sbjct: 309 HWLFFPPLLR 318

[Close]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL
Match: A0A6J1IJ93 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111476790 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.7e-148
Identity = 266/310 (85.81%), Postives = 285/310 (91.94%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSSSFAF +TW
Sbjct: 9   MKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSSFAFIVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSK+SF LFASIAFLPTRIKH+KTAKYVQN +++G +PHPK+
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQNQEEVGKEPHPKI 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PL+LPAKVVIF ML+MGK++ EI+DPIA   +NCA LYFYLDM M LSNVFVRSRFGVEV
Sbjct: 129 PLNLPAKVVIFVMLIMGKKYYEIVDPIAKSCMNCAMLYFYLDMIMCLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
            +PF+EPYLATSLQNFWGRRWNR VSETLH AVYQPVRYE+G PRWMAVV+VFVVSGLMH
Sbjct: 189 SRPFNEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIRVIPTWEVTWFFV+HGLCVAVEIELKRALGKRWRIH AVS PLT  F VAT 
Sbjct: 249 ELLLYYIIRVIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPLTTVFLVATA 308

Query: 301 HWLFFPPLLR 311
           HWL FPPLLR
Sbjct: 309 HWLLFPPLLR 318

[Close]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL
Match: A0A6J1FFC5 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3662 GN=LOC111443440 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.2e-145
Identity = 260/310 (83.87%), Postives = 283/310 (91.29%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           M +ILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSS+FAF +TW
Sbjct: 9   MNMILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSNFAFLVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSK+SF LFASIAFLPTRIKH+KTAKYVQN  ++G +P PK+
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQNQKEVGKEPRPKI 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PL LPAKVVIF ML+MG+++ EI+DPIA L++NCATLYFYLDM M LSNVFVRSRFGVEV
Sbjct: 129 PLKLPAKVVIFVMLIMGQKYYEIVDPIAKLFMNCATLYFYLDMIMCLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
            Q F+EPYLATSLQNFWGRRWN+ VSETLH  VYQPVRYE+G PRWMAVV+VFVVSGLMH
Sbjct: 189 SQSFNEPYLATSLQNFWGRRWNQLVSETLHNVVYQPVRYEVGAPRWMAVVMVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYY IRVIPTWEVTWFFV+HGLC+AVEIELKRALGKRWRIH AVS PLT+AF VAT 
Sbjct: 249 ELLLYYTIRVIPTWEVTWFFVMHGLCLAVEIELKRALGKRWRIHWAVSGPLTMAFLVATA 308

Query: 301 HWLFFPPLLR 311
           HWL FPPLLR
Sbjct: 309 HWLLFPPLLR 318

[Close]

BLAST of CmoCh09G009770 vs. NCBI nr
Match: XP_022937045.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita moschata])

HSP 1 Score: 629.0 bits (1621), Expect = 2.2e-176
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW
Sbjct: 9   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV
Sbjct: 129 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
           RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH
Sbjct: 189 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV
Sbjct: 249 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 308

Query: 301 HWLFFPPLLRIKHFPSG 318
           HWLFFPPLLRIKHFPSG
Sbjct: 309 HWLFFPPLLRIKHFPSG 325

[Close]

BLAST of CmoCh09G009770 vs. NCBI nr
Match: XP_023536379.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 311/317 (98.11%), Postives = 311/317 (98.11%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSSSFAFFITW
Sbjct: 9   MKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSSFAFFITW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHP L
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPML 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PLSLPAKVVIFAMLVMGKRHLEIMDPIA LYVNCATLYFYLDMTMSLSNVFVRSRFGVEV
Sbjct: 129 PLSLPAKVVIFAMLVMGKRHLEIMDPIAKLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
           RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH
Sbjct: 189 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIR IPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV
Sbjct: 249 ELLLYYIIRAIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 308

Query: 301 HWLFFPPLLRIKHFPSG 318
           HWLFFPPLLRIKH PSG
Sbjct: 309 HWLFFPPLLRIKHSPSG 325

[Close]

BLAST of CmoCh09G009770 vs. NCBI nr
Match: XP_022976363.1 (probable long-chain-alcohol O-fatty-acyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 570.5 bits (1469), Expect = 9.2e-159
Identity = 284/317 (89.59%), Postives = 298/317 (94.01%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKV+LISM SLLYCYFIA KL KGKFRLISFLPVFSLFLVLPLYVSSA LSS+ +FF+TW
Sbjct: 9   MKVLLISMVSLLYCYFIAQKLSKGKFRLISFLPVFSLFLVLPLYVSSAFLSSNISFFVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKL+LFSFNLGPLAFD K+S +LF SIAFLPTRIKHNKTAKYVQNHDDLGN+P PKL
Sbjct: 69  LTTFKLILFSFNLGPLAFDPKLSLSLFVSIAFLPTRIKHNKTAKYVQNHDDLGNEPRPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PLSLPAKVVIFAMLVMG RHLEI  PIA LY+NCATLYFYLDM MSLSNVFVRSRFGVEV
Sbjct: 129 PLSLPAKVVIFAMLVMGGRHLEIGGPIAKLYLNCATLYFYLDMIMSLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
           R+PFDEPYLATSLQNFWGRRWN+SVSETLHTAVYQPVRYEIGVPRWMAVV+VFVVSGLMH
Sbjct: 189 RRPFDEPYLATSLQNFWGRRWNQSVSETLHTAVYQPVRYEIGVPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIR IPTWE+TWFFV HGLCVAVEIELKRALGKRWRIH AVSRPLTIAF VAT+
Sbjct: 249 ELLLYYIIRAIPTWEITWFFVAHGLCVAVEIELKRALGKRWRIHWAVSRPLTIAFLVATI 308

Query: 301 HWLFFPPLLRIKHFPSG 318
           HWLFFPPLLRIKH PSG
Sbjct: 309 HWLFFPPLLRIKHSPSG 325

[Close]

BLAST of CmoCh09G009770 vs. NCBI nr
Match: XP_022936880.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 539.7 bits (1389), Expect = 1.7e-149
Identity = 268/310 (86.45%), Postives = 287/310 (92.58%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSSSFAF +TW
Sbjct: 9   MKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSSFAFLVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSK+SF LFASIAFLPTRIKH+KTAKY QN +++G +P PK+
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYTQNQEEVGKEPRPKI 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PL+LPAKVVIF ML+MGK++ EI+DPIA L +NCATLYFYLDM M LSNVFVRSRFGVEV
Sbjct: 129 PLNLPAKVVIFVMLIMGKKYYEIVDPIAKLCMNCATLYFYLDMIMCLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
            +PFDEPYLATSLQNFWGRRWNR VSETLH AVYQPVRYE+G PRWMAVV+VFVVSGLMH
Sbjct: 189 SRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIR IPTWEVTWFFV+HGLCVAVEIELKRALGKRWRIH AVS PLT+AF VAT 
Sbjct: 249 ELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPLTMAFLVATA 308

Query: 301 HWLFFPPLLR 311
           HWLFFPPLLR
Sbjct: 309 HWLFFPPLLR 318

[Close]

BLAST of CmoCh09G009770 vs. NCBI nr
Match: XP_022976365.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 534.6 bits (1376), Expect = 5.6e-148
Identity = 266/310 (85.81%), Postives = 285/310 (91.94%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           MKVILISM SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSA LSSSFAF +TW
Sbjct: 9   MKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSSSFAFIVTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           LTTFKLVLFSFNLGPLAFDSK+SF LFASIAFLPTRIKH+KTAKYVQN +++G +PHPK+
Sbjct: 69  LTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQNQEEVGKEPHPKI 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
           PL+LPAKVVIF ML+MGK++ EI+DPIA   +NCA LYFYLDM M LSNVFVRSRFGVEV
Sbjct: 129 PLNLPAKVVIFVMLIMGKKYYEIVDPIAKSCMNCAMLYFYLDMIMCLSNVFVRSRFGVEV 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGVPRWMAVVIVFVVSGLMH 240
            +PF+EPYLATSLQNFWGRRWNR VSETLH AVYQPVRYE+G PRWMAVV+VFVVSGLMH
Sbjct: 189 SRPFNEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVVVFVVSGLMH 248

Query: 241 ELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATV 300
           ELLLYYIIRVIPTWEVTWFFV+HGLCVAVEIELKRALGKRWRIH AVS PLT  F VAT 
Sbjct: 249 ELLLYYIIRVIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPLTTVFLVATA 308

Query: 301 HWLFFPPLLR 311
           HWL FPPLLR
Sbjct: 309 HWLLFPPLLR 318

[Close]

BLAST of CmoCh09G009770 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 128/318 (40.25%), Postives = 184/318 (57.86%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +KV + ++ S+ YCY+I P++  G  R +S  PV +LFLVLPL+ SS  LS   AFF+TW
Sbjct: 9   IKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITAFFLTW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL      +F  F      P +++ N  +   QNH        PKL
Sbjct: 69  LANFKLILFSFDKGPL-IPIPTNFPRFFCFTCFPIKVQQNPKS---QNH-------LPKL 128

Query: 121 PLSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEV 180
             ++  K+ IFA+L+    + + + P   L +    LY  +++ ++   V V    G ++
Sbjct: 129 VFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDL 188

Query: 181 RQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVR------YEIGVPRWMAVVIVFV 240
               ++PYLATSLQ+FWGRRWN  V   L  AVY P+R         G   +  ++  F+
Sbjct: 189 EPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFI 248

Query: 241 VSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKR--ALGKRWRIHSAVSRPLT 300
           VSGL+HELL +Y+IR +PT EVT FFV+HG+C AVE+ +K+   + +RWR+   VSR LT
Sbjct: 249 VSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLT 308

Query: 301 IAFFVATVHWLFFPPLLR 311
           + F   T  WLF P L R
Sbjct: 309 VGFVFVTGGWLFTPQLKR 313

[Close]

BLAST of CmoCh09G009770 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-50
Identity = 120/315 (38.10%), Postives = 176/315 (55.87%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +K+ + ++ S+ YCY+++  +  G FRL+S LPV +LFLV PL+ S    S   AFF++W
Sbjct: 9   IKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMAFFLSW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL+     + + F  I   P + + N     +QN+         K+
Sbjct: 69  LANFKLILFSFDQGPLS-PLPRTLSRFICITCFPIKPQQNPN---IQNY---------KI 128

Query: 121 PLSLPA-KVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVE 180
           P+ L A KVVIF +L+    + + + P   L  N   ++  L++   L    V    G +
Sbjct: 129 PIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCD 188

Query: 181 VRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVR------YEIGVPRWMAVVIVF 240
           +    +EPYLATSLQ+FWGRRWN  V   L  AVY P R             ++ V   F
Sbjct: 189 LEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAF 248

Query: 241 VVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKR--WRIHSAVSRPL 300
           +VSG +HE+L +Y+ R +PT EVTWFF++HG+C   E+ +K++   R  WR+   VSR L
Sbjct: 249 LVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLL 308

Query: 301 TIAFFVATVHWLFFP 307
           T+ F V T  W FFP
Sbjct: 309 TVGFVVVTSGWFFFP 310

[Close]

BLAST of CmoCh09G009770 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.0e-50
Identity = 123/318 (38.68%), Postives = 175/318 (55.03%), Query Frame = 0


Query: 1   MKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITW 60
           +KV + ++ S+ YCY+ + ++  G FRL+S LPV  LFLVLPL+VSS   S S AFF++W
Sbjct: 9   IKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTAFFLSW 68

Query: 61  LTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKL 120
           L  FKL+LFSF+ GPL F    + + F      P +++ N               P  ++
Sbjct: 69  LANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQNP-------------KPQNQM 128

Query: 121 P-LSLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVE 180
           P      K+  F +L+    + + M P   L +    +Y   ++ ++   V +      +
Sbjct: 129 PKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 188

Query: 181 VRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIG------VPRWMAVVIVF 240
           +   F+EPYL+TSLQ+FWGRRWN  V   L  AVY PVR   G         ++ V   F
Sbjct: 189 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 248

Query: 241 VVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRA-LGKRWRIHSAVSRPLT 300
           +VSG++HEL+ +Y  R  PT EVT FFV+HG+C A E   KR  L +RW++   VSR LT
Sbjct: 249 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLT 308

Query: 301 IAFFVATVHWLFFPPLLR 311
           + F V T  WLFFP L R
Sbjct: 309 VGFVVMTGGWLFFPHLAR 312

[Close]

BLAST of CmoCh09G009770 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 192.6 bits (488), Expect = 4.9e-49
Identity = 112/307 (36.48%), Postives = 172/307 (56.03%), Query Frame = 0


Query: 10  SLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITWLTTFKLVLF 69
           SL Y +FIA  +PKG  RLI F PVF +F ++P  + S  L    AFFI WL  FKL+LF
Sbjct: 14  SLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWLANFKLLLF 73

Query: 70  SFNLGPLAFDSK-VSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKLPLSLPAKV 129
           +   GPL+ + K +S  +F +++ LP +I+ +       +H+        + PL    K 
Sbjct: 74  ALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEG-----STEGPLIYTIKA 133

Query: 130 VIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEVRQPFDEPY 189
           V   +++    +   +     L +    +YF L++ ++ +   VR+   +E+   F++PY
Sbjct: 134 VFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPY 193

Query: 190 LATSLQNFWGRRWNRSVSETLHTAVYQPVRYEIGV--PRW---MAVVIVFVVSGLMHELL 249
           LATSLQ+FWGRRWN  V+  L   VY+P      V  P +   +A    FVVSG+MHEL+
Sbjct: 194 LATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFVVSGIMHELI 253

Query: 250 LYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKRWRIHSAVSRPLTIAFFVATVHWL 309
            +Y+ R+ P W++ WFF+++G C  VEI +K+ +  RWR   A+S+ LT+ F + T  WL
Sbjct: 254 FFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALWL 313

Query: 310 FFPPLLR 311
           F P   R
Sbjct: 314 FLPEFNR 315

[Close]

BLAST of CmoCh09G009770 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-48
Identity = 117/316 (37.03%), Postives = 177/316 (56.01%), Query Frame = 0


Query: 3   VILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSSSFAFFITWLT 62
           V + ++ S+ YCY+I+  +  G  RL S LP+  LF VLPL+ SS   SSS AF+++ + 
Sbjct: 11  VWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSSSTAFYLSEMA 70

Query: 63  TFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQNHDDLGNDPHPKLPL 122
           + KL+LF+F+ GPL F    +   F      P +++ N  ++  QNH       H +   
Sbjct: 71  SLKLILFAFDQGPL-FPVAPNLIQFVCFTCFPIKLQRNPKSQPSQNHF------HKR--- 130

Query: 123 SLPAKVVIFAMLVMGKRHLEIMDPIANLYVNCATLYFYLDMTMSLSNVFVRSRFGVEVRQ 182
           +   K++IF +++    +   +     L +    LY  L++ +    V +    G ++  
Sbjct: 131 AFAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEILLGPLKVLLSMALGCDLEP 190

Query: 183 PFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVR------YEIGVPRWMAVVIVFVVS 242
            F++PYLATSLQ+FWGRRWN  VS  L + +Y PVR         G  R+M  ++ F+VS
Sbjct: 191 QFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRPMNSGWARFMGYLVTFLVS 250

Query: 243 GLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRA--LGKRWRIHSAVSRPLTIA 302
           GL HEL+ +YI R  PTWEVT FFV++G+C   E+ +KR   L + W + S+VSR LT+ 
Sbjct: 251 GLFHELVYFYITRETPTWEVTLFFVLNGVCTGTEVAVKRTAFLQRWWPVRSSVSRLLTMG 310

Query: 303 FFVATVHWLFFPPLLR 311
           F V T   LFFP  +R
Sbjct: 311 FVVVTGGLLFFPLFIR 316

The following BLAST results are available for this feature:

BLAST of CmoCh09G009770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ76	1.7e-51	40.25	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	1.6e-49	38.10	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	2.8e-49	38.68	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	2.4e-48	37.74	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9SV07	6.9e-48	36.48	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]

BLAST of CmoCh09G009770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FFF7	1.1e-176	100.00	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3...	[more]
A0A6J1ILW9	4.5e-159	89.59	probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=366...	[more]
A0A6J1FEY7	8.4e-150	86.45	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IJ93	2.7e-148	85.81	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366...	[more]
A0A6J1FFC5	2.2e-145	83.87	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3...	[more]

BLAST of CmoCh09G009770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022937045.1	2.2e-176	100.00	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita moschata]	[more]
XP_023536379.1	1.5e-172	98.11	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep...	[more]
XP_022976363.1	9.2e-159	89.59	probable long-chain-alcohol O-fatty-acyltransferase 3 [Cucurbita maxima]	[more]
XP_022936880.1	1.7e-149	86.45	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_022976365.1	5.6e-148	85.81	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima]	[more]

BLAST of CmoCh09G009770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55340.1	1.2e-52	40.25	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.2e-50	38.10	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	2.0e-50	38.68	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT3G51970.1	4.9e-49	36.48	acyl-CoA sterol acyl transferase 1	[more]
AT1G34500.1	1.4e-48	37.03	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 183..261 e-value: 7.7E-16 score: 58.1
None	No IPR available	PANTHER	PTHR31595:SF8	(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATED	coord: 1..311
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 1..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh09G009770.1	CmoCh09G009770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity