CmoCh09G004250 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh09G004250
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionHXXXD-type acyl-transferase family protein
LocationCmo_Chr09: 1893978 .. 1898249 (+)
RNA-Seq ExpressionCmoCh09G004250
SyntenyCmoCh09G004250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATACCAACGAACTATCTTCATTTTGTCATGTGGGTATGCGTAAAAATGAACTTTTTACGTATAATTCACCCGAATAGACCATAAAGTTCTTATCTTCTTAAACAACACTAGCAAATTAATCTTCCATCTACCAAATGGGTATATGTAAAGCCATTAATTTGCATGCTTAGCTGCATATTAACACAAGAAATTGTTGATTATATAGTGATTTGGCATAGAATAGAGGAAGTGGAGGGTGTGGAATCTTAGGCAAAATAATACATTATGAGCCAATAGAAGATTGAAGCAAAAGATTGGGGAATTATTATTAGGTAAAGAGCAGAATATAAGGCTAAGTGTTGCCAAAGTTGGAGACATCATTCTTTCTTCTCTACCAATATTTTATGCCTTATGTGCCAATCCAACCTGTTTTTCCAACTCAAAACCCCGCTTACTAAAGTGTTCGTTAACATTTTGGTCCCTGAACTTTTGTAAATTTAATGATTTAGTCTCTAAACTTTAACGATTTAATCTATGTACTTTCAAACTTGTAATAATTCAATTTGCAGACACGCGGGGTTTGCATTATCTTAGTGTTTTTATGGTTTGAAACAATTTATAGGTTAACATGTCGATAAAGAAGCTCGATATTAATAAGATATTATTAACCCTAAACTCGAAATTCTACTTATCAAAGGTTCAGATCTCTCTATTCTCACGAAAGGGTATATGACGGATGAGTAAAAACTATAAACCCTAGAATTCTTGATCGTTGACATATAAAACCTACGTTTTACGTATCATGCACATTTATTCTTATAAAGTTGGATTCATTAACCCTAAACCCTAAATTCTACGTACCGAACACTCAAATCTTTCTATTCTCACGAAAAAGTATTTGACGGATAAGTAAAAGCTATACAAACCCTAGAATTCCTAGTCGTTGACGTATACAACCTACATTGTCAGTTGGATTTATTAACCCTAAACCCTAAATTCTACCGATCAAAAATGCAAATCTTTCTATACAAACCCTAGAATTCCAGGTCGTTGACATACGTAACCTACTTTTTACGTGTGATACATATTTATTACAAAATTGGATTTATTAACCGTAAACCCTAAACTGTACCGATCAAAGATTCAAATCTCTTTATTCTCGCGAAAAAAGTATTTAACGGACGAGTAAAAGCTATATGAACCCTAGAAGTCTTAGCCGTTGATATATACAACCTACTTTTTACGTATTATATACATTTATTCGGAATAGGTGTATAGATATCATTAATAAAAAAAATAAAAAAAATAAAAAAAATAAAAAAAGAAAAAGAAAAGGAATTTTATAATTGTTAATTACCAACCATAACGTAGCACCTAAGTTAATGAAAAGAATTCACAAATTAAATTACAAGTTTAGGGAAACCCCAAACTCCTTATAATTACCCAACAAACTCCTCTTCTCTTCTCCACAGACAAAGACAAAGCCCTTCAAATAAAACAGCACCATAAATGGCCACTCCCCATGTTTCTCTGACAAAATCTCTTACTGTCTTCCCCAAAACCTTAAACCCCTCGAAACTTTTGGAGCTCTCAAATTTAGACAGGCAGTGTCCTTTACATATGTACCTTATTTTCTTCTACAATCCTTCTTCTATTTACACTAACTCATCACTTGATTCAGTGTTCACCAAACTGAAGATTGGTCTGGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCAGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGGTGAGAGGCTGTTTAACTTTTTTGTTGTCAAATTTTACCTTAAAAACATGGTTTTTATTGGTCAGATTTTATTGTATGGGACAAAGAGACAGAGAGACAAAGAGACAAGCTTTGGATTACACAATGAACACATTCCACAGTCTGTAAAGGCCAATTATTTTTAATAATATTGCATTAAAAATTTAGGCTAAATTAATAAAAACACCCAATCTTTTGTAGTTTTAAAAATATAAGCGTTTGGGAAAAAAAAATTCAAAATATTTTTACGGTTAGTTTTGAATAGGAATGTTAATATTTTGTTTAAAAAATACGTTTGAATCTTACGAAAAATGTTTTATTAATACTCTTATATCTTTTAAACCGTTAGTGCCGACTCATTCTTCAACGGGCATACGGTCAAAATCCTAGTATCCTCCTACTGTTTGGAAGCCTACGGTGTCGAGAAAATTTGTATGTATTTTTGAAATATGGTACTAACGGTTTCATTCATAGGTGACAAGATTTGGGTGCGGAGGATACTCCATAGGAACTGGAATAAGCCATTCATTATTCGATGGACCAGCTGCATACGAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCCATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGCTTTTGGGGAAAATGGTGGCTTTCCTTGTTCTTCATTTGATGTTATTGCTGCTCATCTATGGAAGGTACTTCTAACTTATTTTCAAAACTAATGGGAATGTTCGTACAGGCTAATGTTTTTAAAATATGTCGACTAGGAAAAAGTTTTCACACTTTTATAAAAAATGTTTCGTTCTTTTCCTCAACTAACGTAGGATCTCACAATCTACCCCTTTAAGGCCCAGCGTTCTTGCTGACATACTGTCTCATGTCCACCCCCTTCGTGGCCCAGCGTCCTTGCTGACACACTGTCTCATGTCCATCCCCTTTCGAGGCCCAACGTCTTTGCTGACACACCACCTCATGTCCACCTCCCTTCGGAGCTCAGCCTCCATGCTGGCACATTGCCCGATGTCTGGTTCTGATACTGTTTGTAACAACTCAAGCCCCCCTAGCAGATATTGTCCTCTTCGGACTTTCATTTTCAAGCTTCTTCTCAAGGTTTTTAAAACAGCTAGGGAGAGGTTCCCACACTCTTATAAAGAATGTTTCATTCTCCTTCTCAACCGATGTGGGATCTCACAATCCACCCCCCGTTGGAGTCCAATGGATCTCACAATCCACCCCTCTTTGGGGTCCAATGTCCTTATTGGCACACCGCCTTGTGTCCACCCCCTTCGGGGCTCAGCCTCCACGCTGACACATCGCTTGGTGTCTAGTTCTGATACCATTTGTAACAGCTCAAGTTCACTGTTAGCTGATATTGTCCAAAAATGCGTCTGTCAATGAGAGATTTCCACACCCTTACGGAGAATGTTTCATTCTCCTCCTCAACCGATGTAGGATCTCACACCCACCCTAAGCTATTCTCAAATTGATTGTGGAGGGTATCATTTGAAGTTAATTAATGTGTGTTTGCATGGATTTGTTGTTATTTGGAATTAATTAAGTTAATTAAGTTGGGAAAATTGGTTGGAGATTTTGATGTCTATTATTGTGAGTACAGGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATAGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTGGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAATGCATACCAACACGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCAATCCTCGACGTCGCTTACTTTCTGCAGAACCCCACAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACATACTTTTTTACTAGCATGCATTAA

mRNA sequence

AATACCAACGAACTATCTTCATTTTGTCATGTGGTGTTCACCAAACTGAAGATTGGTCTGGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCAGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGGTGACAAGATTTGGGTGCGGAGGATACTCCATAGGAACTGGAATAAGCCATTCATTATTCGATGGACCAGCTGCATACGAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCCATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGCTTTTGGGGAAAATGGTGGCTTTCCTTGTTCTTCATTTGATGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATAGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTGGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAATGCATACCAACACGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCAATCCTCGACGTCGCTTACTTTCTGCAGAACCCCACAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACATACTTTTTTACTAGCATGCATTAA

Coding sequence (CDS)

AATACCAACGAACTATCTTCATTTTGTCATGTGGTGTTCACCAAACTGAAGATTGGTCTGGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCAGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGGTGACAAGATTTGGGTGCGGAGGATACTCCATAGGAACTGGAATAAGCCATTCATTATTCGATGGACCAGCTGCATACGAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCCATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGCTTTTGGGGAAAATGGTGGCTTTCCTTGTTCTTCATTTGATGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATAGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTGGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAATGCATACCAACACGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCAATCCTCGACGTCGCTTACTTTCTGCAGAACCCCACAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACATACTTTTTTACTAGCATGCATTAA

Protein sequence

NTNELSSFCHVVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH
Homology
BLAST of CmoCh09G004250 vs. ExPASy Swiss-Prot
Match: Q9SZ58 (Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT1 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.3e-96
Identity = 198/409 (48.41%), Postives = 270/409 (66.01%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAV-TDVKISELGDLS 71
           VF+ LK+GLEET+SVW PAAGRL  +    KLN+RC++ GA++  AV T VK+SELGDL+
Sbjct: 60  VFSNLKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLT 119

Query: 72  NYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAW 131
            YNEF+E LV+KP LDGDF   PLVVAQVTRF CGGYSIG G SHSLFDG +AYEF+ AW
Sbjct: 120 QYNEFYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAW 179

Query: 132 ATKSAIFKDDKEQYGVELQ----KPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHL 191
           A+ S I      +   + +    KPVH+R  LL+    NR + + +       A AI HL
Sbjct: 180 ASNSHIHNKSNSKITNKKEDVVIKPVHDRRNLLV----NRDAVRET------NAAAICHL 239

Query: 192 YKLIMQSAM------GNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--- 251
           Y+LI Q+ M       N E+    +  +T  ++   IE++KKK+     GF CSSF+   
Sbjct: 240 YQLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLA 299

Query: 252 -----ARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQ-LEEE 311
                ART+AL + ++ +VCLQFAVD R + + PLP+GFSGN++VLASVA  A++ LEE 
Sbjct: 300 AHLWKARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEEL 359

Query: 312 SYKTIIEMIRDAKSQVDDDYVNAYQHGLDGP-QASLPPLKELTVISDWTRMPFHKIEFLH 371
           + ++I+  IR+AK  +D  Y+N+Y   L G    +LPPLKELT+ISDWT+MPFH + F +
Sbjct: 360 TLESIVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGN 419

Query: 372 G--EAAYASPLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF 398
           G   A Y +PL PP+  VAYF++NP D++ + VR+ L  + ++ FS +F
Sbjct: 420 GGEPADYMAPLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHF 455

BLAST of CmoCh09G004250 vs. ExPASy Swiss-Prot
Match: Q94CD1 (Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN=HHT1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.2e-35
Identity = 113/395 (28.61%), Postives = 184/395 (46.58%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-E 75
           +K  L + L  + P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E
Sbjct: 85  IKKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPE 144

Query: 76  FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 135
              +LV+  V   +  E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +
Sbjct: 145 TLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 204

Query: 136 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 195
                     G+ L  P      +L    P +                +   ++ I   +
Sbjct: 205 R---------GLPLTTPPFSDRTILNARNPPKIEN-------------LHQEFEEIEDKS 264

Query: 196 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENG----GFPCSSFD--------ARTKA 255
             N    K+    R+       I+ LK +A  EN     G  C+SF+        ARTK+
Sbjct: 265 NINSLYTKEPTLYRSFCFDPEKIKKLKLQA-TENSESLLGNSCTSFEALSAFVWRARTKS 324

Query: 256 LSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRD 315
           L +  ++   L FAVD R K +P LPKG+ GN  VL +    A +L E+     + ++R+
Sbjct: 325 LKMLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVRE 384

Query: 316 AKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPP 375
           A   V D Y+ +     +  +A  P L    +I+ W+R+ FH  +F  GE   + P+  P
Sbjct: 385 AIKMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALP 444

Query: 376 ILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF 398
             +V  FL +    ++I+V + L +  +D F   F
Sbjct: 445 EKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQF 454

BLAST of CmoCh09G004250 vs. ExPASy Swiss-Prot
Match: Q9FFQ7 (Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FACT PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.2e-30
Identity = 111/387 (28.68%), Postives = 183/387 (47.29%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDV---KISELGDLSNY 75
           +K  L E L  + PAAGRL  +P +GK+ + C   G ++  A  +    KI +     + 
Sbjct: 61  IKKSLSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQ 120

Query: 76  NEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAT 135
            E  E+LV+      +  E P VV QVT F CGG+ +G G++H++FDG AA EFL++WA 
Sbjct: 121 PETLEKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAE 180

Query: 136 KSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAG-----SGAGAVAIDHLY 195
            +          G+ L  P      LL    P +     +        SG G +  D   
Sbjct: 181 TAR---------GLPLSVPPFLDRTLLRPRTPPKIEFPHNEFEDLEDISGTGKLYSDE-- 240

Query: 196 KLIMQSAM-GNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDARTKALSVS 255
           KL+ +S + G E++E+       L++   M ET +   F    GF    + AR +AL + 
Sbjct: 241 KLVYKSFLFGPEKLER-------LKI---MAET-RSTTFQTLTGF---LWRARCQALGLK 300

Query: 256 KEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 315
            ++ + L FA D R++  P LPKG+SGN  V       A ++        + +++ A   
Sbjct: 301 PDQRIKLLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEM 360

Query: 316 VDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 375
           V+D ++ +     +  +A  P L    +I+ W ++ FH  +F  GE   + P+  P  +V
Sbjct: 361 VNDGFMRSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEV 420

Query: 376 AYFLQNPTDSQAIDVRVTLLSQTLDAF 394
             FL   +D+++I+V + L    +  F
Sbjct: 421 ILFLPCGSDTKSINVLLGLPGSAMKVF 420

BLAST of CmoCh09G004250 vs. ExPASy Swiss-Prot
Match: Q8LL69 (3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase OS=Taxus canadensis OX=88032 GN=TAX10 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-25
Identity = 110/394 (27.92%), Postives = 166/394 (42.13%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEF 75
           ++  L + L  + P AGRL      G+L + C   GA+   A+ +  IS L DL + N  
Sbjct: 65  IREALSKVLVYYFPLAGRLRSKEI-GELEVECTGDGALFVEAMVEDTISVLRDLDDLNPS 124

Query: 76  FEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKSA 135
           F++LV    LD    +  LV+ QVTRF CGG ++G  + HS+ DG  A +F++A A  + 
Sbjct: 125 FQQLVFWHPLDTAIEDLHLVIVQVTRFTCGGIAVGVTLPHSVCDGRGAAQFVTALAEMA- 184

Query: 136 IFKDDKEQYGVELQKPVHERGRLLMGSVP--NRCSGKPSAAGSGAGAVAIDHLYKLIMQS 195
                              RG +     P  NR    P            D L+  + Q 
Sbjct: 185 -------------------RGEVKPSLEPIWNRELLNPE-----------DPLHLQLNQF 244

Query: 196 AMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKALSV 255
                    +E G  +  ++   IE +K+    E   F CSSF+        ARTKAL +
Sbjct: 245 DSICPPPMLEELGQASFVINVDTIEYMKQCVMEECNEF-CSSFEVVAALVWIARTKALQI 304

Query: 256 SKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKS 315
              + V L FA+D R    PPLP G+ GN+   A      + L   S    I +I+ AK+
Sbjct: 305 PHTENVKLLFAMDLRKLFNPPLPNGYYGNAIGTAYAMDNVQDLLNGSLLRAIMIIKKAKA 364

Query: 316 QVDDDY----VNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLK- 375
            + D+Y    V    + LD  + S      +  +SDW R+ F++ +F  G     S L+ 
Sbjct: 365 DLKDNYSRSRVVTNPYSLDVNKKS----DNILALSDWRRLGFYEADFGWGGPLNVSSLQR 421

Query: 376 -----PPILDVAYFLQNPTDSQAIDVRVTLLSQT 390
                P      Y L     S  I + ++ +  T
Sbjct: 425 LENGLPMFSTFLYLLPAKNKSDGIKLLLSCMPPT 421

BLAST of CmoCh09G004250 vs. ExPASy Swiss-Prot
Match: Q8S9G6 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=29808 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.9e-24
Identity = 90/353 (25.50%), Postives = 157/353 (44.48%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEF 75
           ++  L + L  + P AGRL     +G L + C   GA+   A+ D ++S LGD  + N  
Sbjct: 66  IREALAKILVYYPPFAGRLR-ETENGDLEVECTGEGAMFLEAMADNELSVLGDFDDSNPS 125

Query: 76  FEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKSA 135
           F++L+    LD +F + PL+V QVTRF CGG+ +G    H + DG  A +FL   A  + 
Sbjct: 126 FQQLLFSLPLDTNFKDLPLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMA- 185

Query: 136 IFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSAM 195
                + +  + L+ P+  R  + +               + +    I   Y +I     
Sbjct: 186 -----RGEVKLSLE-PIWNRELVKLDDPKYLQFFHFEFLRAPSIVEKIVQTYFII----- 245

Query: 196 GNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKALSVSK 255
              + E   Y      + + ++E  K+          CSSF+        ART+A  + +
Sbjct: 246 ---DFETINY------IKQSVMEECKEF---------CSSFEVASAMTWIARTRAFQIPE 305

Query: 256 EKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQV 315
            + V + F +D RN   PPLP G+ GNS   A      + L   S    I +I+ +K  +
Sbjct: 306 SEYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSL 365

Query: 316 DDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLK 361
           +D++ +           ++   + +   +DW+R+ F +++F  G A   SP++
Sbjct: 366 NDNFKSRAVVKPSELDVNMNH-ENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of CmoCh09G004250 vs. ExPASy TrEMBL
Match: A0A6J1F9T5 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443397 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.5e-223
Identity = 391/399 (97.99%), Postives = 391/399 (97.99%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM
Sbjct: 178 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH 403
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH 456

BLAST of CmoCh09G004250 vs. ExPASy TrEMBL
Match: A0A6J1IG59 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476630 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.4e-212
Identity = 378/398 (94.97%), Postives = 380/398 (95.48%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKD+KEQ  VELQKPVHERGRLLMGSVPNRCSGK SAAGSGA AVAIDHLYKLIM
Sbjct: 178 TKSAIFKDNKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIE LKKKAFGENGGF CSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIERLKKKAFGENGGFACSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEK VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQ GLDGPQASLPPL ELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF TS+
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSI 455

BLAST of CmoCh09G004250 vs. ExPASy TrEMBL
Match: A0A6J1IT61 (brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111480377 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 6.7e-176
Identity = 315/404 (77.97%), Postives = 349/404 (86.39%), Query Frame = 0

Query: 6   SSFCHVVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISE 65
           SS    +FT LKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+++ISE
Sbjct: 49  SSSLDSIFTNLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISE 108

Query: 66  LGDLSNYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE 125
           LGDLSNYNEFFE+LV+KPVL+G+F EFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE
Sbjct: 109 LGDLSNYNEFFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE 168

Query: 126 FLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDH 185
           FLSAWA KSAIFKD KE YGVEL  PVHERGRLLM     R S K +   S +GAVAIDH
Sbjct: 169 FLSAWAAKSAIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDH 228

Query: 186 LYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD-------- 245
           LY+LIMQS  G EEM KKEY  RT R+SRGMIE LKK A GENGGF CSSFD        
Sbjct: 229 LYRLIMQSVTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWK 288

Query: 246 ARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTII 305
           ARTKA+SVS EK+VCLQFAV+ARNKL PPLPKGFSGN+FVLASVAL A+QLE+ESYKTI+
Sbjct: 289 ARTKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIV 348

Query: 306 EMIRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYAS 365
           EMIR+AKS+VD+DY+NAY+ GL+GPQASLPPLKELTV+SDWTRMPFHKIEFLHG+A YAS
Sbjct: 349 EMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYAS 408

Query: 366 PLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           PLKPPILDVAYFLQ+P DSQAIDVRV LL  TL+AFS+YF T M
Sbjct: 409 PLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGM 452

BLAST of CmoCh09G004250 vs. ExPASy TrEMBL
Match: A0A6J1FK90 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446510 PE=3 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.3e-175
Identity = 317/404 (78.47%), Postives = 350/404 (86.63%), Query Frame = 0

Query: 6   SSFCHVVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISE 65
           SS    VFTKLKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+V+IS+
Sbjct: 49  SSSFDSVFTKLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISK 108

Query: 66  LGDLSNYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE 125
           LGDLSNYNEFFE+LV+KPVL+G+F EFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE
Sbjct: 109 LGDLSNYNEFFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE 168

Query: 126 FLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDH 185
           FLSAWA KSAIFKD KE YGVEL  PVHERGRLLMG    R   K +   S  GAVAIDH
Sbjct: 169 FLSAWAAKSAIFKDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVS--GAVAIDH 228

Query: 186 LYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD-------- 245
           LY+LIMQS  G EEM KKEY  RT R+SRGMIE LKK A GENGGF CSSFD        
Sbjct: 229 LYRLIMQSMTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWK 288

Query: 246 ARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTII 305
           ARTKA+SVSKEK+VCLQFAV+ARNKL PPLPKGFSGN+FVLASVAL A+QLE+ESYKTI+
Sbjct: 289 ARTKAMSVSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIV 348

Query: 306 EMIRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYAS 365
           +MIR+AKS+VD+DY+NAY+ GL+GPQASLPPLKELTV+SDWTRMPFHKIEFLHG+A YAS
Sbjct: 349 DMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYAS 408

Query: 366 PLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           PLKPPILDVAYFLQ+P DSQAIDVRV LL  TL+AFS+YF T M
Sbjct: 409 PLKPPILDVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGM 450

BLAST of CmoCh09G004250 vs. ExPASy TrEMBL
Match: A0A5A7VFE3 (Omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001100 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.4e-172
Identity = 314/406 (77.34%), Postives = 345/406 (84.98%), Query Frame = 0

Query: 6   SSFCHVVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISE 65
           +S   +VF KLKIGLEETLS+WSPAAGRL+ NP DGKLNLRCDNRGAIL +A+T+VKISE
Sbjct: 15  NSSLDLVFNKLKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKISE 74

Query: 66  LGDLSNYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYE 125
           LGDLSNYN+FFE+LV KPVL+G+F EFPLVVAQVTRFGCGGY+IGTGISHSLFDGPAAY+
Sbjct: 75  LGDLSNYNDFFEKLVQKPVLNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYD 134

Query: 126 FLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDH 185
           FLSAWAT SAIFKDD   Y ++   PVHERGRLL G+V NRCS          G VAIDH
Sbjct: 135 FLSAWATNSAIFKDD--NYKMQTISPVHERGRLLTGTVANRCS----------GTVAIDH 194

Query: 186 LYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGE-NGG-FPCSSFD------ 245
           LYKLIMQSAMGNEE+ +KEY CRT RVS+ MIE LK  A GE NGG F CSSFD      
Sbjct: 195 LYKLIMQSAMGNEEVGRKEYVCRTFRVSKEMIERLKMNALGEINGGAFACSSFDLIAAHL 254

Query: 246 --ARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKT 305
             ARTKALSVSKEK+VCLQFAVDARNKL PPLPKGFSGN+FVLASVAL AKQL+EESYKT
Sbjct: 255 WKARTKALSVSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKT 314

Query: 306 IIEMIRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAY 365
           IIEMI+ AKS+VD+DY+NAYQ GL+GPQASLPPLKELTV+SDWTRMPFHKI+FLHGEAAY
Sbjct: 315 IIEMIQKAKSRVDNDYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAY 374

Query: 366 ASPLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           ASPLKPPIL+VAYFLQNPTD QAIDV V LL  T DAFS+YF T M
Sbjct: 375 ASPLKPPILEVAYFLQNPTDGQAIDVHVPLLEHTFDAFSSYFLTRM 408

BLAST of CmoCh09G004250 vs. NCBI nr
Match: XP_022936964.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 782.3 bits (2019), Expect = 2.0e-222
Identity = 391/399 (97.99%), Postives = 391/399 (97.99%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM
Sbjct: 178 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH 403
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH 456

BLAST of CmoCh09G004250 vs. NCBI nr
Match: KAG6591543.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 765.0 bits (1974), Expect = 3.2e-217
Identity = 384/398 (96.48%), Postives = 385/398 (96.73%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGK SAAGSGAGAVAIDHLYKLIM
Sbjct: 178 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKK FGENGGFPCSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENGGFPCSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEK+VCLQFAVDARNKLKPPLPKGFSGNSFVLASV LPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVTLPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQ GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF TSM
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSM 455

BLAST of CmoCh09G004250 vs. NCBI nr
Match: XP_023535946.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 7.2e-217
Identity = 383/398 (96.23%), Postives = 386/398 (96.98%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILA+AVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILAQAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKP LDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPALDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGK SAAGSGAGAVAIDHLYKLIM
Sbjct: 178 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEK+VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQ GLDGPQA+LPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQLGLDGPQATLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF TSM
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSM 455

BLAST of CmoCh09G004250 vs. NCBI nr
Match: XP_022976161.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 9.1e-212
Identity = 378/398 (94.97%), Postives = 380/398 (95.48%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKD+KEQ  VELQKPVHERGRLLMGSVPNRCSGK SAAGSGA AVAIDHLYKLIM
Sbjct: 178 TKSAIFKDNKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--------ARTKAL 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIE LKKKAFGENGGF CSSFD        ARTKAL
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIERLKKKAFGENGGFACSSFDVIAAHLWKARTKAL 297

Query: 252 SVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 311
           SVSKEK VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA
Sbjct: 298 SVSKEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDA 357

Query: 312 KSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 371
           KSQVDDDYVNAYQ GLDGPQASLPPL ELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI
Sbjct: 358 KSQVDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPI 417

Query: 372 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 402
           LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF TS+
Sbjct: 418 LDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSI 455

BLAST of CmoCh09G004250 vs. NCBI nr
Match: KAG7024428.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675.6 bits (1742), Expect = 2.6e-190
Identity = 349/391 (89.26%), Postives = 350/391 (89.51%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 71
           VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN
Sbjct: 58  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 117

Query: 72  YNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA 131
           YNEFFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWA
Sbjct: 118 YNEFFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWA 177

Query: 132 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIM 191
           TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGK SAAGSGAGAVAIDHLYKLIM
Sbjct: 178 TKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIM 237

Query: 192 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDARTKALSVSKEKIV 251
           QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKK FGEN         ARTKALSVSKEK+V
Sbjct: 238 QSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN-------VQARTKALSVSKEKMV 297

Query: 252 CLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDY 311
           CLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDY
Sbjct: 298 CLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDY 357

Query: 312 VNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQ 371
           VNAYQ GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQ
Sbjct: 358 VNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQ 414

Query: 372 NPTDSQAIDVRVTLLSQTLDAFSTYFFTSMH 403
           NP DSQAIDVRVTLLSQTLDAFSTYF TSMH
Sbjct: 418 NPADSQAIDVRVTLLSQTLDAFSTYFLTSMH 414

BLAST of CmoCh09G004250 vs. TAIR 10
Match: AT4G31910.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 354.8 bits (909), Expect = 9.4e-98
Identity = 198/409 (48.41%), Postives = 270/409 (66.01%), Query Frame = 0

Query: 12  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAV-TDVKISELGDLS 71
           VF+ LK+GLEET+SVW PAAGRL  +    KLN+RC++ GA++  AV T VK+SELGDL+
Sbjct: 60  VFSNLKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLT 119

Query: 72  NYNEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAW 131
            YNEF+E LV+KP LDGDF   PLVVAQVTRF CGGYSIG G SHSLFDG +AYEF+ AW
Sbjct: 120 QYNEFYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAW 179

Query: 132 ATKSAIFKDDKEQYGVELQ----KPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHL 191
           A+ S I      +   + +    KPVH+R  LL+    NR + + +       A AI HL
Sbjct: 180 ASNSHIHNKSNSKITNKKEDVVIKPVHDRRNLLV----NRDAVRET------NAAAICHL 239

Query: 192 YKLIMQSAM------GNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFD--- 251
           Y+LI Q+ M       N E+    +  +T  ++   IE++KKK+     GF CSSF+   
Sbjct: 240 YQLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLA 299

Query: 252 -----ARTKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQ-LEEE 311
                ART+AL + ++ +VCLQFAVD R + + PLP+GFSGN++VLASVA  A++ LEE 
Sbjct: 300 AHLWKARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEEL 359

Query: 312 SYKTIIEMIRDAKSQVDDDYVNAYQHGLDGP-QASLPPLKELTVISDWTRMPFHKIEFLH 371
           + ++I+  IR+AK  +D  Y+N+Y   L G    +LPPLKELT+ISDWT+MPFH + F +
Sbjct: 360 TLESIVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGN 419

Query: 372 G--EAAYASPLKPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF 398
           G   A Y +PL PP+  VAYF++NP D++ + VR+ L  + ++ FS +F
Sbjct: 420 GGEPADYMAPLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHF 455

BLAST of CmoCh09G004250 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-36
Identity = 113/395 (28.61%), Postives = 184/395 (46.58%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-E 75
           +K  L + L  + P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E
Sbjct: 85  IKKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPE 144

Query: 76  FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 135
              +LV+  V   +  E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +
Sbjct: 145 TLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 204

Query: 136 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 195
                     G+ L  P      +L    P +                +   ++ I   +
Sbjct: 205 R---------GLPLTTPPFSDRTILNARNPPKIEN-------------LHQEFEEIEDKS 264

Query: 196 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENG----GFPCSSFD--------ARTKA 255
             N    K+    R+       I+ LK +A  EN     G  C+SF+        ARTK+
Sbjct: 265 NINSLYTKEPTLYRSFCFDPEKIKKLKLQA-TENSESLLGNSCTSFEALSAFVWRARTKS 324

Query: 256 LSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRD 315
           L +  ++   L FAVD R K +P LPKG+ GN  VL +    A +L E+     + ++R+
Sbjct: 325 LKMLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVRE 384

Query: 316 AKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPP 375
           A   V D Y+ +     +  +A  P L    +I+ W+R+ FH  +F  GE   + P+  P
Sbjct: 385 AIKMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALP 444

Query: 376 ILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF 398
             +V  FL +    ++I+V + L +  +D F   F
Sbjct: 445 EKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQF 454

BLAST of CmoCh09G004250 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-36
Identity = 113/395 (28.61%), Postives = 184/395 (46.58%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-E 75
           +K  L + L  + P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E
Sbjct: 69  IKKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPE 128

Query: 76  FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 135
              +LV+  V   +  E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +
Sbjct: 129 TLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVA 188

Query: 136 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 195
                     G+ L  P      +L    P +                +   ++ I   +
Sbjct: 189 R---------GLPLTTPPFSDRTILNARNPPKIEN-------------LHQEFEEIEDKS 248

Query: 196 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENG----GFPCSSFD--------ARTKA 255
             N    K+    R+       I+ LK +A  EN     G  C+SF+        ARTK+
Sbjct: 249 NINSLYTKEPTLYRSFCFDPEKIKKLKLQA-TENSESLLGNSCTSFEALSAFVWRARTKS 308

Query: 256 LSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRD 315
           L +  ++   L FAVD R K +P LPKG+ GN  VL +    A +L E+     + ++R+
Sbjct: 309 LKMLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVRE 368

Query: 316 AKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPP 375
           A   V D Y+ +     +  +A  P L    +I+ W+R+ FH  +F  GE   + P+  P
Sbjct: 369 AIKMVTDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALP 428

Query: 376 ILDVAYFLQNPTDSQAIDVRVTLLSQTLDAFSTYF 398
             +V  FL +    ++I+V + L +  +D F   F
Sbjct: 429 EKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQF 438

BLAST of CmoCh09G004250 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-31
Identity = 103/394 (26.14%), Postives = 175/394 (44.42%), Query Frame = 0

Query: 13  FTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNY 72
           +  +K  L E L  + P AGRL  +P +GK+ + C   G ++  A  +  I  + +  + 
Sbjct: 58  YNVIKKSLSEVLVHYYPVAGRLTISP-EGKIAVNCTGEGVVVVEAEANCGIDTIKEAISE 117

Query: 73  N--EFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAW 132
           N  E  E+LV+      +  E P VV QVT F CGG+ +G G+SH++FDG AA EFL++W
Sbjct: 118 NRMETLEKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMSHNMFDGVAAAEFLNSW 177

Query: 133 ATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLY--- 192
              +          G+ L  P      +L    P +     +             +Y   
Sbjct: 178 CEMAK---------GLPLSVPPFLDRTILRSRNPPKIEFPHNEFDEIEDISDTGKIYDEE 237

Query: 193 KLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDA--------R 252
           KLI +S +   E                 +E LK  A  EN     S+F A        R
Sbjct: 238 KLIYKSFLFEPE----------------KLEKLKIMAIEENNNNKVSTFQALTGFLWKSR 297

Query: 253 TKALSVSKEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEM 312
            +AL    ++ V L FA D R++  P LP+G+ GN  VL  +   + +L        + +
Sbjct: 298 CEALRFKPDQRVKLLFAADGRSRFIPRLPQGYCGNGIVLTGLVTSSGELVGNPLSHSVGL 357

Query: 313 IRDAKSQVDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPL 372
           ++     V D ++ +     +    + P +    +I+ W+++  HK++F  GE  ++ P+
Sbjct: 358 VKRLVELVTDGFMRSAMDYFE-VNRTRPSMNATLLITSWSKLTLHKLDFGWGEPVFSGPV 417

Query: 373 KPPILDVAYFLQNPTDSQAIDVRVTLLSQTLDAF 394
             P  +V  FL +  D ++I+V + L +  ++ F
Sbjct: 418 GLPGREVILFLPSGDDMKSINVFLGLPTSAMEVF 424

BLAST of CmoCh09G004250 vs. TAIR 10
Match: AT5G63560.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.5e-31
Identity = 111/387 (28.68%), Postives = 183/387 (47.29%), Query Frame = 0

Query: 16  LKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDV---KISELGDLSNY 75
           +K  L E L  + PAAGRL  +P +GK+ + C   G ++  A  +    KI +     + 
Sbjct: 61  IKKSLSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQ 120

Query: 76  NEFFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAT 135
            E  E+LV+      +  E P VV QVT F CGG+ +G G++H++FDG AA EFL++WA 
Sbjct: 121 PETLEKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAE 180

Query: 136 KSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAG-----SGAGAVAIDHLY 195
            +          G+ L  P      LL    P +     +        SG G +  D   
Sbjct: 181 TAR---------GLPLSVPPFLDRTLLRPRTPPKIEFPHNEFEDLEDISGTGKLYSDE-- 240

Query: 196 KLIMQSAM-GNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDARTKALSVS 255
           KL+ +S + G E++E+       L++   M ET +   F    GF    + AR +AL + 
Sbjct: 241 KLVYKSFLFGPEKLER-------LKI---MAET-RSTTFQTLTGF---LWRARCQALGLK 300

Query: 256 KEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 315
            ++ + L FA D R++  P LPKG+SGN  V       A ++        + +++ A   
Sbjct: 301 PDQRIKLLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEM 360

Query: 316 VDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 375
           V+D ++ +     +  +A  P L    +I+ W ++ FH  +F  GE   + P+  P  +V
Sbjct: 361 VNDGFMRSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEV 420

Query: 376 AYFLQNPTDSQAIDVRVTLLSQTLDAF 394
             FL   +D+++I+V + L    +  F
Sbjct: 421 ILFLPCGSDTKSINVLLGLPGSAMKVF 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ581.3e-9648.41Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT... [more]
Q94CD12.2e-3528.61Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FFQ72.2e-3028.68Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FA... [more]
Q8LL692.7e-2527.923'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase OS=Taxus canadensis OX=88032 G... [more]
Q8S9G63.9e-2425.50Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=2... [more]
Match NameE-valueIdentityDescription
A0A6J1F9T59.5e-22397.99brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IG594.4e-21294.97brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1IT616.7e-17677.97brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FK903.3e-17578.47brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5A7VFE33.4e-17277.34Omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
XP_022936964.12.0e-22297.99brassinosteroid-related acyltransferase 1-like [Cucurbita moschata][more]
KAG6591543.13.2e-21796.48Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023535946.17.2e-21796.23brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022976161.19.1e-21294.97brassinosteroid-related acyltransferase 1-like [Cucurbita maxima][more]
KAG7024428.12.6e-19089.26Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
AT4G31910.19.4e-9848.41HXXXD-type acyl-transferase family protein [more]
AT5G41040.11.6e-3628.61HXXXD-type acyl-transferase family protein [more]
AT5G41040.21.6e-3628.61HXXXD-type acyl-transferase family protein [more]
AT3G48720.11.2e-3126.14HXXXD-type acyl-transferase family protein [more]
AT5G63560.11.5e-3128.68HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 188..399
e-value: 5.3E-38
score: 132.3
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 2..168
e-value: 2.0E-28
score: 101.3
IPR003480TransferasePFAMPF02458Transferasecoord: 9..395
e-value: 4.2E-47
score: 160.8
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 8..400
NoneNo IPR availablePANTHERPTHR31642:SF145BRASSINOSTEROID-RELATED ACYLTRANSFERASE 1coord: 8..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh09G004250.1CmoCh09G004250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups