CmoCh08G012130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G012130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr08: 7701057 .. 7702855 (-)
RNA-Seq ExpressionCmoCh08G012130
SyntenyCmoCh08G012130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAATGGATTTGACGCCGAAGGTGACAACGAGAAAAAGAAAATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCAACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAGCCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGGGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAACTGAAGAACATGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCCGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCAGCGATCACACCGAATGTGGTGGTGGCGCAAGACGGAAGTGGAAAATATAAGACGATTAATGAAGCGTTGGTTGAGGTACTGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGACGGCATTCAAACCTTCAAGACAGCAACATTTGGTAAGTCAATAAAAGTCTTAATTTTGTTTATATTAAAAAATATTATCTTTTCTTTTAATGTTGTTTTTGTCGCATTTAATTAGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTCGAGAATACCGCTGGTGCGACGAAACATCAAGCCGTGGCGCTTCGAGTCCAATCTGACAGATCAATTTTCTTCAACTGTCATATGGACGGCCACCAAGATACTCTCTACGCCCAAACTCACCGTCAATTTTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCGGGAATGATGTGA

mRNA sequence

ATGTCGAATGGATTTGACGCCGAAGGTGACAACGAGAAAAAGAAAATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCAACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAGCCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGGGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAACTGAAGAACATGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCCGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCAGCGATCACACCGAATGTGGTGGTGGCGCAAGACGGAAGTGGAAAATATAAGACGATTAATGAAGCGTTGGTTGAGGTACTGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGACGGCATTCAAACCTTCAAGACAGCAACATTTGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTCGAGAATACCGCTGGTGCGACGAAACATCAAGCCGTGGCGCTTCGAGTCCAATCTGACAGATCAATTTTCTTCAACTGTCATATGGACGGCCACCAAGATACTCTCTACGCCCAAACTCACCGTCAATTTTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCGGGAATGATGTGA

Coding sequence (CDS)

ATGTCGAATGGATTTGACGCCGAAGGTGACAACGAGAAAAAGAAAATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCAACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAGCCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGGGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAACTGAAGAACATGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCCGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCAGCGATCACACCGAATGTGGTGGTGGCGCAAGACGGAAGTGGAAAATATAAGACGATTAATGAAGCGTTGGTTGAGGTACTGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGACGGCATTCAAACCTTCAAGACAGCAACATTTGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTCGAGAATACCGCTGGTGCGACGAAACATCAAGCCGTGGCGCTTCGAGTCCAATCTGACAGATCAATTTTCTTCAACTGTCATATGGACGGCCACCAAGATACTCTCTACGCCCAAACTCACCGTCAATTTTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCGGGAATGATGTGA

Protein sequence

MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM
Homology
BLAST of CmoCh08G012130 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 654.4 bits (1687), Expect = 1.1e-186
Identity = 307/573 (53.58%), Postives = 413/573 (72.08%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MS G+D E    K++  ++ +SS+ L++MVVAVTVGV+ + H G+        G  E+++
Sbjct: 1   MSYGYDDE-SKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGD------SKGKAEVNA 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           S KA++ +C PTDY++TCE++L+  G NTTDP ELVK  F V M Q+  A + S T+  L
Sbjct: 61  SVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMEL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
            K+ R   AL  C+EL++YA+D++++SF  +G F+   LD+ + +L +WLS A+++E+TC
Sbjct: 121 QKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           L+GF+ T G+AGE MKK LK+A E+T NGLAI++E+ N +G +Q      R L  +  PS
Sbjct: 181 LEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPS 240

Query: 241 WVSDEKRRLMQVPTA--AITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIY 300
           WV    R+L+Q   A   + P++VVAQDGSG+YKTINEAL  V KK N TFV+++KAG+Y
Sbjct: 241 WVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLY 300

Query: 301 KETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENT 360
           KE V + K M+H+   GDGP KT++SG+ N+ DGI T++TAT A+VG  F  +++GFENT
Sbjct: 301 KEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENT 360

Query: 361 AGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAV 420
           AGA KHQAVA+RVQSD SIFFNC  DG+QDTLY  +HRQF+RDC +SGTIDF+FG++AAV
Sbjct: 361 AGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAV 420

Query: 421 FQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRP 480
           FQNC L+VRKPL NQ C +TA GR + RE TG VFQ C   G+P YL  K  +KAYLGRP
Sbjct: 421 FQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRP 480

Query: 481 WKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKT 540
           WK++SRTIIM + I D +QP+GW PW+GDFGL TLFY+E QN GPG+ L NRV W GIKT
Sbjct: 481 WKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKT 540

Query: 541 LTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           L+ +    FTP  +I GDDWI   GVPY+ G++
Sbjct: 541 LSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CmoCh08G012130 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.2e-183
Identity = 305/570 (53.51%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MS G+D E    KK+  ++ +SS+ L++MVVAVT+GV  S +K +  G+       EI++
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGV--SVNKSDNAGD------EEITT 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           S KAI+ +C PTDYKETCE++L  +  +T+DP ELVK  F   M Q++   + S T+  L
Sbjct: 61  SVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIEL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
            K+PRA  AL  C+EL++YAI +++ SF  +G F+  K+D+ +  L +WLS  +++EQTC
Sbjct: 121 QKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGF+ T G+AGE +KK LK+A ++T NGLA+VTE+ N LG +Q  +   R L   E PS
Sbjct: 181 LDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           W+    RRL+  P + + P++VVAQDGSG+YKTINEAL  V KK N TFV+++K GIYKE
Sbjct: 241 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
            V + ++MTH+   GDGP KTV+SGS ++ DGI T+KTAT A+VG +F  +++ FENTAG
Sbjct: 301 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           A KHQAVA+RV +D SIF+NC  DG+QDTLYA +HRQFYRDC +SGTIDF+FG++AAVFQ
Sbjct: 361 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NC L+VRKPL NQ C +TA GR + RE TG V Q C  +G+P YL  K  +K YLGRPWK
Sbjct: 421 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           ++SRTIIM + I D + PEGW PW+G+FGLNTLFY+E QN GPGA +T RV W GIK L+
Sbjct: 481 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGM 571
            ++   FTP  +I GD WI   GVPY  G+
Sbjct: 541 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh08G012130 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 3.2e-165
Identity = 285/570 (50.00%), Postives = 386/570 (67.72%), Query Frame = 0

Query: 8   EGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQT 67
           +G+ +KKK  + GV +  LV MVVAV +  + ++   E +         +I ++T A++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKI------VPVQIKTATTAVEA 63

Query: 68  ICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRN-STTLQALAKEPRA 127
           +C PTDYKETC  SLM    ++T P +L+K+GF V +  +  +I+  S  L A A   + 
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 128 NQ-ALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFE 187
            + AL+ C +L+  A DD+    +    F + +++ FVEDL VWLSG++ Y+QTC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 188 NTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQ--------GSKAGRRLLAGDE 247
            T     + M+K  K+++E+TSNGLA++T + N+LG           G  A + L A D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 243

Query: 248 VPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGI 307
           +PSWV    RRLM      +  NVVVA DGSG+YKTINEAL  V K +   FVIY+K G+
Sbjct: 244 IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGV 303

Query: 308 YKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDG-IQTFKTATFAVVGANFFCRDMGFE 367
           Y E V + K MTHVT  GDGPTKT ++GSLN+  G ++T+ TAT A+ G NF  +++GFE
Sbjct: 304 YNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFE 363

Query: 368 NTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSA 427
           NTAG   HQAVALRV +D ++F+NC +DG+QDTLY  +HRQF+RDC VSGT+DF+FG+  
Sbjct: 364 NTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGI 423

Query: 428 AVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLG 487
            V QNCN+VVRKP+ +Q C++TAQGR + RE TGLV QNCH  G+PAY+P K++NKAYLG
Sbjct: 424 VVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLG 483

Query: 488 RPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGI 547
           RPWK+FSRTIIMG+ IDD+I P GWLPW GDF LNTL+YAE++N GPG++   RVKW GI
Sbjct: 484 RPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGI 543

Query: 548 KTLTPQQAADFTPKIFIDGDDWILNTGVPY 567
           K L+P+QA  FTP  F+ G+ WI    VPY
Sbjct: 544 KKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmoCh08G012130 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 541.6 bits (1394), Expect = 1.1e-152
Identity = 278/575 (48.35%), Postives = 377/575 (65.57%), Query Frame = 0

Query: 7   AEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQ 66
           ++GD +KKK  + G  S FLV MVV+V V  +  S K         D    I  +TKA+Q
Sbjct: 3   SDGD-KKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPK---------DDENHIRKTTKAVQ 62

Query: 67  TICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEP 126
            +C PTD+K+TC  SLM    ++ DP +L+K+GFKV +  +N ++ + S  ++A A K P
Sbjct: 63  AVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNP 122

Query: 127 RANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGF 186
            A  A + C +L+  AIDD+    +    F + +++ FVEDL VWLSG++ ++QTC+D F
Sbjct: 123 EAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSF 182

Query: 187 ENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNML--GSLQG-----SKAGRRLLA-GD 246
                +  + M K  K+++E++SN LA+VT +  ++   +L G     +K  R+LL+  D
Sbjct: 183 GEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED 242

Query: 247 EVPSWVSDEKRRLMQVPTAAITP---NVVVAQDGSGKYKTINEALVEVLKKSNITFVIYV 306
            +P+WV  E RRLM        P   N VVAQDG+G++KTI +AL  V K + + F+I++
Sbjct: 243 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHI 302

Query: 307 KAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNF-VDGIQTFKTATFAVVGANFFCRD 366
           K GIYKE V + K M HVT  GDGP KT+++GSLNF +  ++TF TAT  + G +F  ++
Sbjct: 303 KEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKN 362

Query: 367 MGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVF 426
           +G ENTAG    QAVALRV +D ++F +C +DGHQDTLY  +HRQFYRDC VSGT+DF+F
Sbjct: 363 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 422

Query: 427 GNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNK 486
           G++  + QNC +VVRKP   Q C+VTAQGR   RE TGLV   CH  GDPAY+P K+VNK
Sbjct: 423 GDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNK 482

Query: 487 AYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVK 546
           AYLGRPWK+FSRTIIM + IDD+I P GWLPW GDF L TL+YAE  N GPG++   RVK
Sbjct: 483 AYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVK 542

Query: 547 WQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYS 568
           W GIK LTPQ A  +T   F+ GD WI  T VPY+
Sbjct: 543 WPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of CmoCh08G012130 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 6.6e-134
Identity = 236/450 (52.44%), Postives = 304/450 (67.56%), Query Frame = 0

Query: 130 LQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTG 189
           ++ C E+L+YA+D I  S   +  F   KL ++  DL VWL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 190 DAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSK--AGRRLLAGDEVPSWVSDEKR 249
            AGE M K LK++ E++SN + ++  +  +L     S+    RRLL+ D +PSWV+D  R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 250 RLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVLIPKA 309
           RL+      + PN VVAQDGSG++KT+ +AL  V  K+ + FVI+VKAG+YKETV + K 
Sbjct: 121 RLL--AGGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 310 MTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATKHQAV 369
           M +VT+ GDGPTKT  +GSLN+ DGI T+ TATF V GANF  +D+GFENTAG  KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 370 ALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVR 429
           ALRV +D++IF+NC MDG QDTLY Q+ RQFYRDC +SGTIDFVFG    VFQNC LV R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 430 KPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTII 489
            P   QQC+VTA GR +    + LVFQ+ HF G+PA L       +YLGRPWKQ+S+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 490 MGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADF 549
           M S ID +  PEG++PWMG     T  Y E+ NKGPGAD   RVKW G+K LT   AA++
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 550 TPKIFID------GDDWILNTGVPYSPGMM 572
            P  F +       D WI+ +GVPYS G M
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of CmoCh08G012130 vs. ExPASy TrEMBL
Match: A0A6J1ER80 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS
Sbjct: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL
Sbjct: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
           AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC
Sbjct: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS
Sbjct: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE
Sbjct: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
           TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG
Sbjct: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ
Sbjct: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK
Sbjct: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT
Sbjct: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           PQQAADFTPKIFIDGDDWILNTGVPYSPGMM
Sbjct: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of CmoCh08G012130 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 541/571 (94.75%), Postives = 557/571 (97.55%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MSNGFDAEGDN+KKKMA VGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISS
Sbjct: 1   MSNGFDAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISS 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           STKAIQTICRPTDYKETCE+SLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL
Sbjct: 61  STKAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
           AKEPR NQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLS+WLSGALTYEQTC
Sbjct: 121 AKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGFENTTGDAGE+MK FLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS
Sbjct: 181 LDGFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           WVS EKRRLMQ+P +AITPNVVVAQDGSGKYKTINEALV+V KKSNITFVIYVKAGIYKE
Sbjct: 241 WVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
           TVLIPKA+THVTMYGDGPTKT+VSG+LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG
Sbjct: 301 TVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           + KHQAVALRVQSDRSIFFNC MDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ
Sbjct: 361 SAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NCNLVVRKPLANQQCIVTAQGRLE REPTG VFQ CH MGDPAYLPFKAVNKAYLGRPWK
Sbjct: 421 NCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           Q+SRTIIMGSEIDDLIQPEGWLPWMGDFGLN+LFYAEFQNKGPGADLTNRVKWQGIKTLT
Sbjct: 481 QYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTLT 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
            QQAADFTPK+FIDGD WILNTGVPYSPGMM
Sbjct: 541 AQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of CmoCh08G012130 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 5.1e-254
Identity = 429/575 (74.61%), Postives = 491/575 (85.39%), Query Frame = 0

Query: 1   MSNGF-DAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEIS 60
           MSNGF +AEGD +KKK+A++ VSSL LVAMVVAVTVG+NN+ ++  G G+       EIS
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEIS 60

Query: 61  SSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQA 120
           SSTKAIQ IC+PTDYKETCE+SLM+E  NTTDP+ELVK+GFK+    LN AIRNSTTLQ 
Sbjct: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120

Query: 121 LAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQT 180
           L+K+PR NQALQNCRELLEYAIDD+ +SF RIGTFQM+KLD+FV DL +WLSGALTYEQT
Sbjct: 121 LSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLAGD-- 240
           CLDGFENTTGDAG +M++FLKSAQEMT+NGL IV EL  +LGSLQ     GRRLL  D  
Sbjct: 181 CLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDH 240

Query: 241 EVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAG 300
           E+PSW+SD KRRLMQ   A + P+++VAQDGSGKYKTIN AL +V  KSN TFVIYVKAG
Sbjct: 241 EMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFE 360
           IYKE V+IPK MTH+TMYGDGPTKTVV+GSLNF+DGIQTFKTATF+ +GANF+ RDMGFE
Sbjct: 301 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360

Query: 361 NTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSA 420
           NTAG+ KHQAVALRVQSDRSIF+NC +DG+QDTLYA  HRQFYRDC +SGTIDFVFGN+A
Sbjct: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420

Query: 421 AVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLG 480
             FQNC LVVRKP+ NQQCIVTA GR+  +EPT L+FQ CHFMGDPAYLPFK++NKAYLG
Sbjct: 421 TNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLG 480

Query: 481 RPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGI 540
           RPWKQ+SRTII+GS+IDD+IQPEGWLPWMGDFGLNTLFYAE QNKGPGA  T RVKW+GI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGI 540

Query: 541 KTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           K +TPQ AADFTP+ FIDGD WI   G+PYS GMM
Sbjct: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of CmoCh08G012130 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.9e-253
Identity = 433/579 (74.78%), Postives = 494/579 (85.32%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHK----GEGLGNG----HG 60
           MSNGFDAE D +KKK+A++ VSSL LVAMVVAVTVGVNN+  +    G G G G    HG
Sbjct: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60

Query: 61  DGAGEISSSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIR 120
           D A +ISSSTKAI+TIC+PTDYKETCEESLM E  NTTDP+ELVK GFK+ +++LN+AIR
Sbjct: 61  DDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120

Query: 121 NSTTLQALAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSG 180
           NSTTLQ LA++PRANQALQNCRELLEYAIDD+ DS++RIGTF ++K ++FV DL +WLS 
Sbjct: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRL 240
           ALTYEQTCLDGFENTTGD+GE+MK+FLKSAQEMT+NGL IV+EL  +LG+L    A  R 
Sbjct: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGRR 240

Query: 241 LAGDEVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIY 300
           L   ++P WVSD KRRLMQV T AI P++VVAQDGSGKYKTINEALV V  KSN TFVIY
Sbjct: 241 LLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300

Query: 301 VKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRD 360
           VKAGIYKE V+IPK MTH+TMYGDGPTK+VV+G+LNFVDGIQTFKTATFA +GANFF RD
Sbjct: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360

Query: 361 MGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVF 420
           MGF NTAG TKHQAVALRVQSDR+I +NC +DG+QDTLYA  +RQFYRDC +SGTIDFVF
Sbjct: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420

Query: 421 GNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNK 480
           GNSAA+FQNC LVVRKPL NQQCIVTA GRL  REPT L+FQ CHFMGDP+YLPFK VNK
Sbjct: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480

Query: 481 AYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVK 540
           AYLGRPWKQ+SRTIIMGS+IDDLIQPEGWLPWMGDF LNTLFYAE QN+GPGAD ++RVK
Sbjct: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540

Query: 541 WQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           W+GI+ +TPQ AA +T + FIDGD WI   GVPYS GMM
Sbjct: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of CmoCh08G012130 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.6e-252
Identity = 433/576 (75.17%), Postives = 491/576 (85.24%), Query Frame = 0

Query: 1   MSNGF-DAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHG-DGAGEI 60
           MSNGF +AEGD +KKK+A++ VSSL LVAMVVAVTVGVNN+ H  E   + HG +   EI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  SSSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQ 120
           +SSTKAIQ IC+PTDYKETCE+SL +E  NTTDP+ELVK+GFK+    LN AI+NSTTL+
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ALAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQ 180
            LAK+PR NQALQNCRELLEYAIDD+  SF RIGTFQM+KLD FV DL +WLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLAGD- 240
           TCLDGFENTTGDAG +M++FLKSAQ+MT+NGL IV EL  +LGSLQ    +GRRLL  D 
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 -EVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKA 300
            E+PSWVSD KRRLMQ   AA+ P++VVAQDGSGKYKTIN AL +V  KSN TFVI+VKA
Sbjct: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300

Query: 301 GIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGF 360
           GIYKE V+IPK MTH+TMYGDGPTKTVV+GSLNF+DGIQTFKTATF+ +GANF+ RDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNS 420
           ENTAGA KHQAVALRVQSDRSIFFNC +DG+QDTLYA  HRQFYRDC +SGTIDFVFGN+
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 AAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYL 480
           A  FQNC LVVRKPL NQQCIVTA GRL  +EPT L+FQ+CHFMGDPAYLPFKA+NKAYL
Sbjct: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480

Query: 481 GRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQG 540
           GRPWK++SRTII+GS IDD+IQPEGWLPWMGDFGLNTLFYAE QNKG GAD + RVKW+G
Sbjct: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540

Query: 541 IKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           IK +TPQ AADFTP+ FIDGD WI   G+PYS GMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of CmoCh08G012130 vs. NCBI nr
Match: XP_022930359.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS
Sbjct: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL
Sbjct: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
           AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC
Sbjct: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS
Sbjct: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE
Sbjct: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
           TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG
Sbjct: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ
Sbjct: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK
Sbjct: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT
Sbjct: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           PQQAADFTPKIFIDGDDWILNTGVPYSPGMM
Sbjct: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of CmoCh08G012130 vs. NCBI nr
Match: XP_023515122.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 556/571 (97.37%), Postives = 565/571 (98.95%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MSNGFDAEGDN+KKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISS
Sbjct: 1   MSNGFDAEGDNKKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISS 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL
Sbjct: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
           AKEPR NQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC
Sbjct: 121 AKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSK GRRLLAGDEVPS
Sbjct: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKKGRRLLAGDEVPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           WV+DEKRRLMQ+PTAAITPNVVVAQDGSGKYKTINEALVEV KKSNITFVIYVKAGIYKE
Sbjct: 241 WVTDEKRRLMQLPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
           TVLIPKA+THVTMYGDGPTKTVVSG LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG
Sbjct: 301 TVLIPKALTHVTMYGDGPTKTVVSGGLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           + KHQAVALRVQSDRSIFFNC MDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ
Sbjct: 361 SAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NC+LVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK
Sbjct: 421 NCSLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKW+GIKTLT
Sbjct: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWKGIKTLT 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           PQQAADFTPKIFIDGDDWILNTGVPYSPGMM
Sbjct: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of CmoCh08G012130 vs. NCBI nr
Match: KAG6593983.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 549/556 (98.74%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 16  MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQTICRPTDYK 75
           MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60

Query: 76  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRANQALQNCRE 135
           ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR NQALQNCRE
Sbjct: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120

Query: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 195
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM
Sbjct: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180

Query: 196 KKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 255
           KKFLKSAQEMTSNGLAIVTELKN LGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA
Sbjct: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240

Query: 256 AITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 315
           AITPNVVVAQDGSGKYKTINEALVEV KKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG
Sbjct: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300

Query: 316 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATKHQAVALRVQSDR 375
           DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG+ KHQAVALRVQSDR
Sbjct: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360

Query: 376 SIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 435
           SIFFNCHMDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420

Query: 436 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 495
           IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL
Sbjct: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480

Query: 496 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 555
           IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG
Sbjct: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540

Query: 556 DDWILNTGVPYSPGMM 572
           DDWILNTGVPYSPGMM
Sbjct: 541 DDWILNTGVPYSPGMM 556

BLAST of CmoCh08G012130 vs. NCBI nr
Match: XP_023000149.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 541/571 (94.75%), Postives = 557/571 (97.55%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MSNGFDAEGDN+KKKMA VGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISS
Sbjct: 1   MSNGFDAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISS 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           STKAIQTICRPTDYKETCE+SLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL
Sbjct: 61  STKAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
           AKEPR NQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLS+WLSGALTYEQTC
Sbjct: 121 AKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGFENTTGDAGE+MK FLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS
Sbjct: 181 LDGFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           WVS EKRRLMQ+P +AITPNVVVAQDGSGKYKTINEALV+V KKSNITFVIYVKAGIYKE
Sbjct: 241 WVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
           TVLIPKA+THVTMYGDGPTKT+VSG+LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG
Sbjct: 301 TVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           + KHQAVALRVQSDRSIFFNC MDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ
Sbjct: 361 SAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NCNLVVRKPLANQQCIVTAQGRLE REPTG VFQ CH MGDPAYLPFKAVNKAYLGRPWK
Sbjct: 421 NCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           Q+SRTIIMGSEIDDLIQPEGWLPWMGDFGLN+LFYAEFQNKGPGADLTNRVKWQGIKTLT
Sbjct: 481 QYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTLT 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
            QQAADFTPK+FIDGD WILNTGVPYSPGMM
Sbjct: 541 AQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of CmoCh08G012130 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 887.9 bits (2293), Expect = 4.7e-254
Identity = 430/575 (74.78%), Postives = 494/575 (85.91%), Query Frame = 0

Query: 1   MSNGF-DAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEIS 60
           MSNGF +AEGD +KKKMA++ VSSL LVAMVVAVTVGVNN+    E  G+GHG    EIS
Sbjct: 1   MSNGFANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPK--EEHGDGHGGETHEIS 60

Query: 61  SSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQA 120
           S+TKAIQ IC+PTDYK+ CE++LM+E  NTTDP+EL K+G ++    LN AIRNSTTLQ 
Sbjct: 61  STTKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQE 120

Query: 121 LAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQT 180
           LAK+PR NQALQNCRELLEYAIDD+ +SF RIGTFQM+KLD+FV DL +WLSGALTYEQT
Sbjct: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLA--GD 240
           CLDGFENTTGDAG +M++FLKS+Q+MT+NGLAIV EL  MLGSLQ     GRRLL    D
Sbjct: 181 CLDGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDAD 240

Query: 241 EVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAG 300
           E+PSWVS+ KRRLMQ   A++ P++VVAQDGSGKYKTIN AL +V  KSN TFVIYVKAG
Sbjct: 241 EMPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFE 360
           IYKE V+IPKAMTH+TMYGDGPTKT+V+GSLNF+DGIQTFKTATFA +GANF+ RDMGFE
Sbjct: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFE 360

Query: 361 NTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSA 420
           NTAGA KHQAVALRVQSDRSIF+NC +DG+QDTLYA  HRQFYRDC +SGTIDFVFGN+A
Sbjct: 361 NTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAA 420

Query: 421 AVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLG 480
            +FQNC LVVRKP+ NQQCIVTA GRL  +EPT L+FQ+CHFMGDPAYLPFK +NKAYLG
Sbjct: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLG 480

Query: 481 RPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGI 540
           RPWKQ+SRTII+GS+IDD+IQPEGWLPWMGDFGLNTLFYAE QNKGPGAD + RVKW+GI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGI 540

Query: 541 KTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           + +TPQ AAD+TP+ FIDGD WI   G+PYS GMM
Sbjct: 541 RHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of CmoCh08G012130 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 654.4 bits (1687), Expect = 8.2e-188
Identity = 307/573 (53.58%), Postives = 413/573 (72.08%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MS G+D E    K++  ++ +SS+ L++MVVAVTVGV+ + H G+        G  E+++
Sbjct: 1   MSYGYDDE-SKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGD------SKGKAEVNA 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           S KA++ +C PTDY++TCE++L+  G NTTDP ELVK  F V M Q+  A + S T+  L
Sbjct: 61  SVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMEL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
            K+ R   AL  C+EL++YA+D++++SF  +G F+   LD+ + +L +WLS A+++E+TC
Sbjct: 121 QKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           L+GF+ T G+AGE MKK LK+A E+T NGLAI++E+ N +G +Q      R L  +  PS
Sbjct: 181 LEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPS 240

Query: 241 WVSDEKRRLMQVPTA--AITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIY 300
           WV    R+L+Q   A   + P++VVAQDGSG+YKTINEAL  V KK N TFV+++KAG+Y
Sbjct: 241 WVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLY 300

Query: 301 KETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENT 360
           KE V + K M+H+   GDGP KT++SG+ N+ DGI T++TAT A+VG  F  +++GFENT
Sbjct: 301 KEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENT 360

Query: 361 AGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAV 420
           AGA KHQAVA+RVQSD SIFFNC  DG+QDTLY  +HRQF+RDC +SGTIDF+FG++AAV
Sbjct: 361 AGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAV 420

Query: 421 FQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRP 480
           FQNC L+VRKPL NQ C +TA GR + RE TG VFQ C   G+P YL  K  +KAYLGRP
Sbjct: 421 FQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRP 480

Query: 481 WKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKT 540
           WK++SRTIIM + I D +QP+GW PW+GDFGL TLFY+E QN GPG+ L NRV W GIKT
Sbjct: 481 WKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKT 540

Query: 541 LTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 572
           L+ +    FTP  +I GDDWI   GVPY+ G++
Sbjct: 541 LSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CmoCh08G012130 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 644.4 bits (1661), Expect = 8.4e-185
Identity = 305/570 (53.51%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 60
           MS G+D E    KK+  ++ +SS+ L++MVVAVT+GV  S +K +  G+       EI++
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGV--SVNKSDNAGD------EEITT 60

Query: 61  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 120
           S KAI+ +C PTDYKETCE++L  +  +T+DP ELVK  F   M Q++   + S T+  L
Sbjct: 61  SVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIEL 120

Query: 121 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 180
            K+PRA  AL  C+EL++YAI +++ SF  +G F+  K+D+ +  L +WLS  +++EQTC
Sbjct: 121 QKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTC 180

Query: 181 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPS 240
           LDGF+ T G+AGE +KK LK+A ++T NGLA+VTE+ N LG +Q  +   R L   E PS
Sbjct: 181 LDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPS 240

Query: 241 WVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKE 300
           W+    RRL+  P + + P++VVAQDGSG+YKTINEAL  V KK N TFV+++K GIYKE
Sbjct: 241 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 300

Query: 301 TVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG 360
            V + ++MTH+   GDGP KTV+SGS ++ DGI T+KTAT A+VG +F  +++ FENTAG
Sbjct: 301 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 360

Query: 361 ATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQ 420
           A KHQAVA+RV +D SIF+NC  DG+QDTLYA +HRQFYRDC +SGTIDF+FG++AAVFQ
Sbjct: 361 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 420

Query: 421 NCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWK 480
           NC L+VRKPL NQ C +TA GR + RE TG V Q C  +G+P YL  K  +K YLGRPWK
Sbjct: 421 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 480

Query: 481 QFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLT 540
           ++SRTIIM + I D + PEGW PW+G+FGLNTLFY+E QN GPGA +T RV W GIK L+
Sbjct: 481 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 540

Query: 541 PQQAADFTPKIFIDGDDWILNTGVPYSPGM 571
            ++   FTP  +I GD WI   GVPY  G+
Sbjct: 541 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh08G012130 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 583.2 bits (1502), Expect = 2.3e-166
Identity = 285/570 (50.00%), Postives = 386/570 (67.72%), Query Frame = 0

Query: 8   EGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQT 67
           +G+ +KKK  + GV +  LV MVVAV +  + ++   E +         +I ++T A++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKI------VPVQIKTATTAVEA 63

Query: 68  ICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRN-STTLQALAKEPRA 127
           +C PTDYKETC  SLM    ++T P +L+K+GF V +  +  +I+  S  L A A   + 
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 128 NQ-ALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFE 187
            + AL+ C +L+  A DD+    +    F + +++ FVEDL VWLSG++ Y+QTC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 188 NTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQ--------GSKAGRRLLAGDE 247
            T     + M+K  K+++E+TSNGLA++T + N+LG           G  A + L A D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 243

Query: 248 VPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGI 307
           +PSWV    RRLM      +  NVVVA DGSG+YKTINEAL  V K +   FVIY+K G+
Sbjct: 244 IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGV 303

Query: 308 YKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDG-IQTFKTATFAVVGANFFCRDMGFE 367
           Y E V + K MTHVT  GDGPTKT ++GSLN+  G ++T+ TAT A+ G NF  +++GFE
Sbjct: 304 YNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFE 363

Query: 368 NTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSA 427
           NTAG   HQAVALRV +D ++F+NC +DG+QDTLY  +HRQF+RDC VSGT+DF+FG+  
Sbjct: 364 NTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGI 423

Query: 428 AVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLG 487
            V QNCN+VVRKP+ +Q C++TAQGR + RE TGLV QNCH  G+PAY+P K++NKAYLG
Sbjct: 424 VVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLG 483

Query: 488 RPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGI 547
           RPWK+FSRTIIMG+ IDD+I P GWLPW GDF LNTL+YAE++N GPG++   RVKW GI
Sbjct: 484 RPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGI 543

Query: 548 KTLTPQQAADFTPKIFIDGDDWILNTGVPY 567
           K L+P+QA  FTP  F+ G+ WI    VPY
Sbjct: 544 KKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmoCh08G012130 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 541.6 bits (1394), Expect = 7.7e-154
Identity = 278/575 (48.35%), Postives = 377/575 (65.57%), Query Frame = 0

Query: 7   AEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQ 66
           ++GD +KKK  + G  S FLV MVV+V V  +  S K         D    I  +TKA+Q
Sbjct: 3   SDGD-KKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPK---------DDENHIRKTTKAVQ 62

Query: 67  TICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEP 126
            +C PTD+K+TC  SLM    ++ DP +L+K+GFKV +  +N ++ + S  ++A A K P
Sbjct: 63  AVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNP 122

Query: 127 RANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGF 186
            A  A + C +L+  AIDD+    +    F + +++ FVEDL VWLSG++ ++QTC+D F
Sbjct: 123 EAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSF 182

Query: 187 ENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNML--GSLQG-----SKAGRRLLA-GD 246
                +  + M K  K+++E++SN LA+VT +  ++   +L G     +K  R+LL+  D
Sbjct: 183 GEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED 242

Query: 247 EVPSWVSDEKRRLMQVPTAAITP---NVVVAQDGSGKYKTINEALVEVLKKSNITFVIYV 306
            +P+WV  E RRLM        P   N VVAQDG+G++KTI +AL  V K + + F+I++
Sbjct: 243 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHI 302

Query: 307 KAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNF-VDGIQTFKTATFAVVGANFFCRD 366
           K GIYKE V + K M HVT  GDGP KT+++GSLNF +  ++TF TAT  + G +F  ++
Sbjct: 303 KEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKN 362

Query: 367 MGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVF 426
           +G ENTAG    QAVALRV +D ++F +C +DGHQDTLY  +HRQFYRDC VSGT+DF+F
Sbjct: 363 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 422

Query: 427 GNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNK 486
           G++  + QNC +VVRKP   Q C+VTAQGR   RE TGLV   CH  GDPAY+P K+VNK
Sbjct: 423 GDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNK 482

Query: 487 AYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVK 546
           AYLGRPWK+FSRTIIM + IDD+I P GWLPW GDF L TL+YAE  N GPG++   RVK
Sbjct: 483 AYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVK 542

Query: 547 WQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYS 568
           W GIK LTPQ A  +T   F+ GD WI  T VPY+
Sbjct: 543 WPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of CmoCh08G012130 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 466.5 bits (1199), Expect = 3.1e-131
Identity = 276/705 (39.15%), Postives = 372/705 (52.77%), Query Frame = 0

Query: 15  KMALVGVSSLFLVAMVVAVTVGVNNS-SHKGEGLGNGHGDGAGEISSSTKAIQTICRPTD 74
           K  L+GV++L ++AMV+ V     NS S K E +   H +    I+ +T +I  IC  TD
Sbjct: 3   KYVLLGVTAL-IMAMVICVEANDGNSPSRKMEEV---HRESKLMITKTTVSI--ICASTD 62

Query: 75  YKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEPRANQALQ 134
           YK+ C  SL +  S   DPR L++  F + +  + S I R    L++ A  +    +AL 
Sbjct: 63  YKQDCTTSLATVRS--PDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALN 122

Query: 135 NCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDA 194
            CREL++ AIDD+  + ++   F   +L  FVEDL VWLSG++TY+QTC+DGFE    +A
Sbjct: 123 TCRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEA 182

Query: 195 GEQMKKFLKSAQEMTSNGLAIVTELKNML------------------------------G 254
              M++ ++  Q +TSNGLAI   L  +L                              G
Sbjct: 183 AVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVG 242

Query: 255 SLQGSKAG---------------------------------------------------- 314
           S Q S                                                       
Sbjct: 243 SSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPK 302

Query: 315 ------------------------------------------------------------ 374
                                                                       
Sbjct: 303 KSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLD 362

Query: 375 ----RRLLAGDEVPSWVSDEKRRLM--QVPTAAITPNVVVAQDGSGKYKTINEALVEVLK 434
               R L    E P WV+   RRL+  +     I  NVVVA+DGSGK KTI +AL  V  
Sbjct: 363 SLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPM 422

Query: 435 KSNITFVIYVKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFV-DGIQTFKTATFA 494
           K+   FVI++K G+YKE V + K M HV   GDGPTKTV++G + F+ D + T++TA+ A
Sbjct: 423 KNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVA 482

Query: 495 VVGANFFCRDMGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDC 554
           V G  F  +D+GFENTAGA +HQAVALRV +D ++FFNCHM+G+QDTLY  THRQFYR+C
Sbjct: 483 VNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNC 542

Query: 555 KVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDP 568
           +VSGTIDFVFG++ AVFQNC  V+R+P+ +QQCIVTAQGR + RE TG+V  N    GD 
Sbjct: 543 RVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDA 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX861.1e-18653.58Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.2e-18353.51Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ213.2e-16550.00Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA11.1e-15248.35Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Q429206.6e-13452.44Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ER800.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1[more]
A0A6J1KJ310.0e+0094.75Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1[more]
A0A1S3C7B65.1e-25474.61Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1[more]
A0A6J1H4H81.9e-25374.78Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1[more]
A0A0A0KD241.6e-25275.17Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022930359.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata][more]
XP_023515122.10.0e+0097.37probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo][more]
KAG6593983.10.0e+0098.74putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp... [more]
XP_023000149.10.0e+0094.75probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima][more]
XP_038875843.14.7e-25474.78probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G05610.18.2e-18853.58Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.18.4e-18553.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.12.3e-16650.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.17.7e-15448.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.13.1e-13139.15Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 60..212
e-value: 1.6E-38
score: 143.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 61..216
e-value: 3.3E-25
score: 86.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 64..212
e-value: 1.3E-28
score: 100.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 260..556
e-value: 9.3E-139
score: 461.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 254..570
e-value: 7.4E-115
score: 385.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 59..218
e-value: 2.8E-36
score: 126.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 63..216
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 16..570
NoneNo IPR availablePANTHERPTHR31707:SF337PECTINESTERASEcoord: 16..570
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 67..216
e-value: 7.97488E-47
score: 159.526
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 404..413
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 255..569

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G012130.1CmoCh08G012130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity