CmoCh08G010810 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G010810
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein SIEVE ELEMENT OCCLUSION B-like
LocationCmo_Chr08: 6957833 .. 6960935 (+)
RNA-Seq ExpressionCmoCh08G010810
SyntenyCmoCh08G010810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCTTCCTGTTTACATTTGTTCTTAGTTTCCCAATCCCTCCTTTGTGAAATCATGTCTCTATCAGTTCCCAACAACCCCGTTGCTTCATTGCACCATCCAAAGCTTTCAACCTCCACCAAGGACGACCAAAGCATAAGTCACTTATCCGATGAGACAGTGACCGACTACATTTATACTAAACATCATGAGGACGATAGTGTGAAGATCGACGTCGATAATTACATTGCACTCGTTGAAAGTATCATCACAACTGCTGACCGAATCACCGAAACTGTTACTCACGTACCCATTTCTACATATCATCGTCTATTTTCTCGTCATTAATTTCTATCTTTCGAGTTTAGTTTATGTATGTATGTATGTGATTGATCCATAACCAAAATTGTAGGGCACCGAGGGCCGTTTGATATTCTCAGATGATTTCTTGAAGGTCAATGCTGTTGATCCACCACTTTGCACCCTTCACAAAGTGTCAAGCCAGGTCATTCTCAAAAATTAATATCTAACCAATCATTAAATGATTAAATATTTCCAAATCATTTTCTTGACGATTTTTAAACATACAAAAGCTTTAGGTTAAGAAAAGAAGTTAGCATGGGACATTCATATATGTATTAATTGGTGAAATGAATTGTGTATCAGCTGTCTTGCAAAGCTCCAGGTGTAGAACAAGCGCACAAGACGACCCTTGACATTCTTGATATATTGGCAAGTTACTCATGGGAGGCTAAAGCAGTGCTAACATTGACTGCCTTTGCGGCTGAATACGGAGACATATGGCACCTCAACCATTACTCATCGTTGGATCCACTCGCAAAATCATTGTCAATGATCAAGCGAGTTCCTTTGTTGAAGAAGCAATTGGAGGGTATGAAGTACCGGCAAGTGCTTGTTAGCCCCAACAGTTTGATCTACAGCTGCTTGCGGGCCATGAAATACATTACCCAACTCAAGAATTTCTCCAAATATGATGTCGATGAACTGCCGGAGTTATCTTCCGTCCGCCGCCAAATTCCTTTCTTTTCTTATTGGATTATTCATATTATCGTTGCTACCCGAATTGAAATTTCAAGTTACTTGAATGAGACCGAGTAAGTTCATAATTATTCTTCTCTTGTTTATCTCCATTGTTTTAAATCACTTTTTTCATATTTATGAAATCATATTGGTAATTCATTCTTTATTGAATGAATTTATTTTAATTTTTCAAAAATAAAACAATGTAGCTTTTCAAGCTCAACTTTTTGCCAAATTTATTTGATTCTTCAATCAAACTCAATGATTTTGTAATAATATTTTCAGGGGCCAATCACAAAGGTATCTGAATGACTTGGCCGAAAAGATCAACCGTTTACTCTGTACGATTGAAGATTATCTCAACGTCATTCGAGAACAACAAGGTCAATTTTCAATTTAAACATGTTTGGAGAGAAGAGAGAGTAAAATATCTTTTCAACCAAAACTTAGATTTTTACTACATATGTATATATATTATGGATGCAGAGGAGATTGATCTTTATAGATGGCTTGTGGGTCACATTAATAATTCTCCCACTCGGATAACTTTAGTTCTGCCTAAGCTTATTGAAGGCAAGGTCGAGGCAAAGCCTTTCATAGATGGTTCTACTCGATTGCAGGTCTTTGCCTTAAATTTTATAGTACAAACGAACATTTTGTTTTTATAATTAGATATATTTAACATACACACGTGATATCTTATGCGTTTGATTTTTTTTTTTTTTTATTTAACATACACACGTGATATCTTATGCGTTTGTAGGTTAGTGTTGAAGATGCTTTGAGAGACAAGAACGTGATCTTGTTAATTTCTGGATTAGATATCTCGGATGATGATATTAGAGCTGTTGATTTAGTTTACAATGAAGTGAGAAAGGAGGATAAGTATAAGATTGTTTGGATTCCAGTTATTCCTGATTATTTTCGAGAAGAAGAAGCTCGAAAGATATATGAGTACTTAAGCTCTTTAATGAAGTGGTACATTGTGCCATACACAACAAAGATTGCAGGTTGGAGATATCTGGAGGACAAATGGCAATTTAGACAAGACCCATTAGTTGTTGTTATGAACTCCGAATCAAAGGTTGAATTTGCAAATGCAATCCATTTAATCCGAGTTTGGGGAGCTGAAGCCATTCCGTTCACGATGGATAGAACTCTTGCTTTATTGAAAAAGAATTGGCCTGAGTCCACCCTTCTAAAATTTATTGAGGAACCAAGATTGCGAAGTTGGGTATGTAAATATAACGTTTTTTCATTAATTTCCACATATTTATGCGTTTAGCAATGTTGTTTTGACTCGTACTTCATGTCTTGACATATCCAATATTTCATCTTTGTTAGGTCAATCAAGATAGGACCATCATATTTTATGGAGGAAAAGATCCAAGTTGGATTCAACAATTCGAAGAGAAAGTAGTAGAAATTAAGAACGACCCATATACTAAAGAAAAAGGATATACATTTGAAATTGTTGAGGTGAATGATTTTACATTGACACCTCATTTTTGGATCACACAATGGGGTTATTTCGTAATCAAGAGTCAAATAAAAGATTCAAGTGCAACTGAAACAACGGAAGATATTTTACGACTAATATCTTATAAAGATGAAAATGGTTGGGCAGTTTTAACAGTTGGCTCAGCTCCATTGGTTGTAGGTCGAGGCGATTTGATTTTGGGAGTTCTCGAAGATTTTAACAAATGGAAAACAACTTTGACAGCCTTCCATGATGTCTTTAAAGACAAGTTCGGCCTGCAAAGCCTGTTATATCGCCCATGCGAAAGAATTACACTTCCTCAATTTAGTGGGTGGATTCCAATAAAAGTAAATTGTCCTGAATGTCCTCTCTTCATGAAGGTTGGTATAAGCTTGAAGTGTAACAGCGGGGGTCGTGAGGACTAATGATGTCAACAATAATATATTGTTTATAATACTATATGTACTACTACTAAATAATATGAGGTGATCCGACAACATGGAATCCTACTATTTGTATCATCAAGTTTGAGCCTGTTATGCTACAACTAAACGTGATTATTTCTTATCGAGGAAAGCCTGTTGGTTCTTCTTTCTCTTCTCC

mRNA sequence

CCCTCTTCCTGTTTACATTTGTTCTTAGTTTCCCAATCCCTCCTTTGTGAAATCATGTCTCTATCAGTTCCCAACAACCCCGTTGCTTCATTGCACCATCCAAAGCTTTCAACCTCCACCAAGGACGACCAAAGCATAAGTCACTTATCCGATGAGACAGTGACCGACTACATTTATACTAAACATCATGAGGACGATAGTGTGAAGATCGACGTCGATAATTACATTGCACTCGTTGAAAGTATCATCACAACTGCTGACCGAATCACCGAAACTGTTACTCACGGCACCGAGGGCCGTTTGATATTCTCAGATGATTTCTTGAAGGTCAATGCTGTTGATCCACCACTTTGCACCCTTCACAAAGTGTCAAGCCAGCTGTCTTGCAAAGCTCCAGGTGTAGAACAAGCGCACAAGACGACCCTTGACATTCTTGATATATTGGCAAGTTACTCATGGGAGGCTAAAGCAGTGCTAACATTGACTGCCTTTGCGGCTGAATACGGAGACATATGGCACCTCAACCATTACTCATCGTTGGATCCACTCGCAAAATCATTGTCAATGATCAAGCGAGTTCCTTTGTTGAAGAAGCAATTGGAGGGTATGAAGTACCGGCAAGTGCTTGTTAGCCCCAACAGTTTGATCTACAGCTGCTTGCGGGCCATGAAATACATTACCCAACTCAAGAATTTCTCCAAATATGATGTCGATGAACTGCCGGAGTTATCTTCCGTCCGCCGCCAAATTCCTTTCTTTTCTTATTGGATTATTCATATTATCGTTGCTACCCGAATTGAAATTTCAAGTTACTTGAATGAGACCGAGGGCCAATCACAAAGGTATCTGAATGACTTGGCCGAAAAGATCAACCGTTTACTCTGTACGATTGAAGATTATCTCAACGTCATTCGAGAACAACAAGAGGAGATTGATCTTTATAGATGGCTTGTGGGTCACATTAATAATTCTCCCACTCGGATAACTTTAGTTCTGCCTAAGCTTATTGAAGGCAAGGTCGAGGCAAAGCCTTTCATAGATGGTTCTACTCGATTGCAGGTTAGTGTTGAAGATGCTTTGAGAGACAAGAACGTGATCTTGTTAATTTCTGGATTAGATATCTCGGATGATGATATTAGAGCTGTTGATTTAGTTTACAATGAAGTGAGAAAGGAGGATAAGTATAAGATTGTTTGGATTCCAGTTATTCCTGATTATTTTCGAGAAGAAGAAGCTCGAAAGATATATGAGTACTTAAGCTCTTTAATGAAGTGGTACATTGTGCCATACACAACAAAGATTGCAGGTTGGAGATATCTGGAGGACAAATGGCAATTTAGACAAGACCCATTAGTTGTTGTTATGAACTCCGAATCAAAGGTTGAATTTGCAAATGCAATCCATTTAATCCGAGTTTGGGGAGCTGAAGCCATTCCGTTCACGATGGATAGAACTCTTGCTTTATTGAAAAAGAATTGGCCTGAGTCCACCCTTCTAAAATTTATTGAGGAACCAAGATTGCGAAGTTGGGTCAATCAAGATAGGACCATCATATTTTATGGAGGAAAAGATCCAAGTTGGATTCAACAATTCGAAGAGAAAGTAGTAGAAATTAAGAACGACCCATATACTAAAGAAAAAGGATATACATTTGAAATTGTTGAGGTGAATGATTTTACATTGACACCTCATTTTTGGATCACACAATGGGGTTATTTCGTAATCAAGAGTCAAATAAAAGATTCAAGTGCAACTGAAACAACGGAAGATATTTTACGACTAATATCTTATAAAGATGAAAATGGTTGGGCAGTTTTAACAGTTGGCTCAGCTCCATTGGTTGTAGGTCGAGGCGATTTGATTTTGGGAGTTCTCGAAGATTTTAACAAATGGAAAACAACTTTGACAGCCTTCCATGATGTCTTTAAAGACAAGTTCGGCCTGCAAAGCCTGTTATATCGCCCATGCGAAAGAATTACACTTCCTCAATTTAGTGGGTGGATTCCAATAAAAGTAAATTGTCCTGAATGTCCTCTCTTCATGAAGGTTGGTATAAGCTTGAAGTGTAACAGCGGGGGTCGTGAGGACTAATGATGTCAACAATAATATATTGTTTATAATACTATATGTACTACTACTAAATAATATGAGGTGATCCGACAACATGGAATCCTACTATTTGTATCATCAAGTTTGAGCCTGTTATGCTACAACTAAACGTGATTATTTCTTATCGAGGAAAGCCTGTTGGTTCTTCTTTCTCTTCTCC

Coding sequence (CDS)

ATGTCTCTATCAGTTCCCAACAACCCCGTTGCTTCATTGCACCATCCAAAGCTTTCAACCTCCACCAAGGACGACCAAAGCATAAGTCACTTATCCGATGAGACAGTGACCGACTACATTTATACTAAACATCATGAGGACGATAGTGTGAAGATCGACGTCGATAATTACATTGCACTCGTTGAAAGTATCATCACAACTGCTGACCGAATCACCGAAACTGTTACTCACGGCACCGAGGGCCGTTTGATATTCTCAGATGATTTCTTGAAGGTCAATGCTGTTGATCCACCACTTTGCACCCTTCACAAAGTGTCAAGCCAGCTGTCTTGCAAAGCTCCAGGTGTAGAACAAGCGCACAAGACGACCCTTGACATTCTTGATATATTGGCAAGTTACTCATGGGAGGCTAAAGCAGTGCTAACATTGACTGCCTTTGCGGCTGAATACGGAGACATATGGCACCTCAACCATTACTCATCGTTGGATCCACTCGCAAAATCATTGTCAATGATCAAGCGAGTTCCTTTGTTGAAGAAGCAATTGGAGGGTATGAAGTACCGGCAAGTGCTTGTTAGCCCCAACAGTTTGATCTACAGCTGCTTGCGGGCCATGAAATACATTACCCAACTCAAGAATTTCTCCAAATATGATGTCGATGAACTGCCGGAGTTATCTTCCGTCCGCCGCCAAATTCCTTTCTTTTCTTATTGGATTATTCATATTATCGTTGCTACCCGAATTGAAATTTCAAGTTACTTGAATGAGACCGAGGGCCAATCACAAAGGTATCTGAATGACTTGGCCGAAAAGATCAACCGTTTACTCTGTACGATTGAAGATTATCTCAACGTCATTCGAGAACAACAAGAGGAGATTGATCTTTATAGATGGCTTGTGGGTCACATTAATAATTCTCCCACTCGGATAACTTTAGTTCTGCCTAAGCTTATTGAAGGCAAGGTCGAGGCAAAGCCTTTCATAGATGGTTCTACTCGATTGCAGGTTAGTGTTGAAGATGCTTTGAGAGACAAGAACGTGATCTTGTTAATTTCTGGATTAGATATCTCGGATGATGATATTAGAGCTGTTGATTTAGTTTACAATGAAGTGAGAAAGGAGGATAAGTATAAGATTGTTTGGATTCCAGTTATTCCTGATTATTTTCGAGAAGAAGAAGCTCGAAAGATATATGAGTACTTAAGCTCTTTAATGAAGTGGTACATTGTGCCATACACAACAAAGATTGCAGGTTGGAGATATCTGGAGGACAAATGGCAATTTAGACAAGACCCATTAGTTGTTGTTATGAACTCCGAATCAAAGGTTGAATTTGCAAATGCAATCCATTTAATCCGAGTTTGGGGAGCTGAAGCCATTCCGTTCACGATGGATAGAACTCTTGCTTTATTGAAAAAGAATTGGCCTGAGTCCACCCTTCTAAAATTTATTGAGGAACCAAGATTGCGAAGTTGGGTCAATCAAGATAGGACCATCATATTTTATGGAGGAAAAGATCCAAGTTGGATTCAACAATTCGAAGAGAAAGTAGTAGAAATTAAGAACGACCCATATACTAAAGAAAAAGGATATACATTTGAAATTGTTGAGGTGAATGATTTTACATTGACACCTCATTTTTGGATCACACAATGGGGTTATTTCGTAATCAAGAGTCAAATAAAAGATTCAAGTGCAACTGAAACAACGGAAGATATTTTACGACTAATATCTTATAAAGATGAAAATGGTTGGGCAGTTTTAACAGTTGGCTCAGCTCCATTGGTTGTAGGTCGAGGCGATTTGATTTTGGGAGTTCTCGAAGATTTTAACAAATGGAAAACAACTTTGACAGCCTTCCATGATGTCTTTAAAGACAAGTTCGGCCTGCAAAGCCTGTTATATCGCCCATGCGAAAGAATTACACTTCCTCAATTTAGTGGGTGGATTCCAATAAAAGTAAATTGTCCTGAATGTCCTCTCTTCATGAAGGTTGGTATAAGCTTGAAGTGTAACAGCGGGGGTCGTGAGGACTAA

Protein sequence

MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAHKTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWIIHIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLVGHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDDIRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVNDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRGDLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECPLFMKVGISLKCNSGGRED
Homology
BLAST of CmoCh08G010810 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 8.0e-54
Identity = 180/703 (25.60%), Postives = 315/703 (44.81%), Query Frame = 0

Query: 32  SDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHG-----TEGRLIFS 91
           SDE++   +  + H  D+ ++ V   ++LVE I+  A   +E          TE +L+ S
Sbjct: 38  SDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATLDSEDTNASMLPLPTEDKLMQS 97

Query: 92  DDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAHKTTLDILDILASYSWEAKAVLTLTAF 151
                ++ +D     + +V+ +++ K+     +H+ T+ + + L+S+ W+ K VLTL AF
Sbjct: 98  S---MMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAF 157

Query: 152 AAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSLIYSCLRAMK 211
           A  YG+ W L  + S + LAKSL+M+K VP        ++ R  L S +  +   +R MK
Sbjct: 158 ALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--------VQNRVTLESVSQGLNDLIREMK 217

Query: 212 YIT----QLKNF-SKYDVDELPELSSVRRQIPFFSYWIIHIIVA--TRIEISSYLNETEG 271
            +T    +L     +Y   ++P+LS +   IP   YW I  ++A  ++I + + +     
Sbjct: 218 SVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITAMGHEMM 277

Query: 272 QSQRYLNDLAEKINRLLCTIEDYLNVIR------EQQEEIDLYRWLVGHINNSPTRITLV 331
            +Q  L + +   N+L    +     +R      E+Q   +  + L    + +      +
Sbjct: 278 NTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTTHIDNMKI 337

Query: 332 LPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDDIRAVDLVYNEVRK 391
           L  L+  K    P  DG T+ +V + D LR K V+LLIS L+I  D++   + +Y E R+
Sbjct: 338 LTALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLISDLNILQDELSIFEQIYTESRR 397

Query: 392 ---------EDKYKIVWIPV---IPDYFREEEARKIYEYLSSLMKWYIV--PYTTKIAGW 451
                       Y++VW+PV   I D+ R    +K +E L   M WY V  P   +    
Sbjct: 398 NLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHVV 457

Query: 452 RYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPEST 511
            ++  +W F   P++VV++ +      NA+H+I +WG EA PFT  R   L ++      
Sbjct: 458 EFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSLN 517

Query: 512 LLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQF---------------EEKVVEIKNDP 571
           L+    +  + +W+  D  I  YGG D  WI++F               E   V  +N  
Sbjct: 518 LIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYVGKRNHS 577

Query: 572 YTKEKGYTFEIVEVNDFT-------LTPHFWITQWGYFVIKSQI-KDSSATETTEDILRL 631
           + ++     E++   + +       L   FW         K Q+ K     +  + I ++
Sbjct: 578 HREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVMQGIKKI 637

Query: 632 ISYKDENGWAVLTVGSAPLVVGRGDLILGVLEDFNKWKTTL------TAFHDVFKDKFGL 672
           +SY    GWA+L+ G   +++  G +   +      WKT +       A  D   D+   
Sbjct: 638 LSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMSDHHHDEVLR 697

BLAST of CmoCh08G010810 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 5.2e-37
Identity = 165/715 (23.08%), Postives = 306/715 (42.80%), Query Frame = 0

Query: 31  LSDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHGTEGRLIFSDDFL 90
           LSD+ V      K H  D +  DV + +++V  I  +   +    +   +  L+F  D+ 
Sbjct: 136 LSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKS--HVPSIDSSAPKPSLVFK-DYA 195

Query: 91  KVNAVDPPLCTLHKVSSQLSCKAPGVEQAH-------------KTTLDILDILASYSWEA 150
              + +     + ++S ++ CK     ++H              TT  +L +++ Y W+A
Sbjct: 196 DHTSFETFADLIDQISCEIDCKCLHGGESHGMMTSGLHLDSRNTTTFSVLSLVSKYRWDA 255

Query: 151 KAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSL 210
           K VL L+A A +YG    L    + + L KSL++IK++P +  +   +  R  L     L
Sbjct: 256 KLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLPSIFSRQNALHQR--LDKTRIL 315

Query: 211 IYSCLRAMKYITQLKNFSKYDVDELPE---LSSVRRQIPFFSYWIIHIIVATRIEIS--- 270
           +   +     I         D+ +LP     ++    IP   YWI+  ++     IS   
Sbjct: 316 MQDMVDLTTTI--------IDIYQLPPNHITAAFTDHIPTAVYWIVRCVLICVSHISGAS 375

Query: 271 -------------SYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRW 330
                        S ++E   + ++    L E+  +   TIE+   +I E+ +E+     
Sbjct: 376 GFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIEE--GIIEEEYQELIQTFT 435

Query: 331 LVGHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISD 390
            + H++  P  + L+ P      ++      G ++ +V + + L  K+V+LLIS L+  +
Sbjct: 436 TIIHVDVVPPLLRLLRP------IDFLYHGAGVSKRRVGI-NVLTQKHVLLLISDLENIE 495

Query: 391 DDIRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIV--PYTTKI 450
            ++  ++ +Y E  ++  ++I+W+PV  D++ E +  K +E L   M+WY++  P   + 
Sbjct: 496 KELYILESLYTEAWQQ-SFEILWVPV-QDFWTEADDAK-FEALHMNMRWYVLGEPRKLRR 555

Query: 451 AGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLAL-LKKNW 510
           A  R++ + W F+  P++V ++ + +V   NA  ++ +W   A PFT  R   L  ++ W
Sbjct: 556 AAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHPFTTARERDLWSEQEW 615

Query: 511 PESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFE--------------EKVVEIK 570
               L+   +   L   V+  + I  YGG+D  WI+ F               E V   K
Sbjct: 616 NLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQWIKNFTSLWRNVAKAANIQLEMVYVGK 675

Query: 571 NDPYTKEKGYTFEIVEVN------DFTLTPHFWITQWGYFVIKSQI------------KD 630
            +P    +     I E N      D      FW      +  K ++            K+
Sbjct: 676 RNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESMWESKQRMLKAHGIKGREGFKE 735

Query: 631 SSATETTEDILRLISYKDE-NGWAVLTVGSAPLVVGRGDLILGVLEDFNKW------KTT 672
                  ++++ ++ Y  E +GW +++  S  +V  +G+L    L +FN+W      K  
Sbjct: 736 EEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGNLFSRGLAEFNEWEVNIPTKGF 795

BLAST of CmoCh08G010810 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 4.3e-23
Identity = 135/618 (21.84%), Postives = 259/618 (41.91%), Query Frame = 0

Query: 26  QSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHGTEGRLIF 85
           + IS L+++ + + +  + H+ D   +D +  +  VE+I++        V      R + 
Sbjct: 5   RDISALNEDIIVEQL-LRSHDPDGRWLDSEMLLQEVETILS-------FVLQNDVSRPLL 64

Query: 86  SDDFLKVNAVDPPLCTL----HKVSSQLSCKAPGVEQAHKTTLDILDILASYSWEAKAVL 145
           +++ +    V     TL     ++S Q+ C   G  +  K T+ + D+L  Y W+AKAVL
Sbjct: 65  TENCITTIEVFDSKETLPYAIFRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAVL 124

Query: 146 TLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSLIYSC 205
            L   AA YG +    H +  DP+A S++ + ++P     +E  K+R  L S N LI + 
Sbjct: 125 VLGVLAATYGGLLLPVHLAICDPVAASIAKLNQLP-----IERTKFRPWLESLNLLIKAM 184

Query: 206 LRAMKYITQLKNF----SKYDVDELPE-LSSV--------------RRQIPFFSY-WIIH 265
           +   K I + +      +K D + L E LS++               +QIP+F       
Sbjct: 185 VDVTKCIIKFEKIPFKQAKLDNNILGETLSNIYLTTYRVVKSALTCMQQIPYFKQTQQAK 244

Query: 266 IIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLVG 325
               T  E+S       G+       L     RL   +ED    I E+  +       + 
Sbjct: 245 KSRKTAAELSIESRRAAGELSSLGYQLLNIHTRLNKQVEDCSTQIEEEINQ------RLR 304

Query: 326 HINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDDI 385
           +IN    +    +  L+    +  P    S ++ ++    ++DK  +LL+S   +     
Sbjct: 305 NINIETHQDNQDVLHLLFSLQDDLPLQQYSRQISIT---EVQDKVTLLLLSKPPVEPLFF 364

Query: 386 RAVDLV--YNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIV--PYTTKIA 445
               L    +    E  Y+I+W+P+       +E ++I+++ S+ + W  V  P+     
Sbjct: 365 LLQQLYDHPSNTNTEQNYEIIWVPIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSST 424

Query: 446 GWRYLEDKWQFR-QDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN-W 505
              + + +W ++  + ++VV++S  +    NA+ ++ +WG +A PF++ R   L K++ W
Sbjct: 425 ILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGW 484

Query: 506 PESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEI 565
             + LL  I          + R I  +G ++  WI +F     +I+N       G+  E+
Sbjct: 485 SINLLLDGIHP------TFEGREICIFGSENLDWIDEFVSLARKIQN------LGFQLEL 544

Query: 566 VEVNDF----------------TLTPHFWITQWGYFVIKSQ---IKDSSATETTEDILRL 593
           + +++                 TL   FW+        K +   I+ S      E++  L
Sbjct: 545 IYLSNQRRDERAMEESSILFSPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNL 588

BLAST of CmoCh08G010810 vs. ExPASy TrEMBL
Match: A0A6J1EQZ1 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436750 PE=4 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV
Sbjct: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
           GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420
           IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420

Query: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480
           YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL
Sbjct: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480

Query: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540
           LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND
Sbjct: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540

Query: 541 FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600
           FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG
Sbjct: 541 FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600

Query: 601 DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP 660
           DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP
Sbjct: 601 DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP 660

Query: 661 LFMKVGISLKCNSGGRED 679
           LFMKVGISLKCNSGGRED
Sbjct: 661 LFMKVGISLKCNSGGRED 678

BLAST of CmoCh08G010810 vs. ExPASy TrEMBL
Match: A0A6J1EX23 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436942 PE=4 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 585/692 (84.54%), Postives = 620/692 (89.60%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNP+ASLHHPKLSTSTKDDQS+ HLSD TVT +IYTKH EDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPIASLHHPKLSTSTKDDQSVRHLSDNTVTGHIYTKHREDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPG+EQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGIEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDIL SYSWEAKAVLTLTAFA EYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILVSYSWEAKAVLTLTAFATEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYD  EL  LSSV RQIP  SYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDTKELSVLSSVLRQIPLVSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVA RIEISSYLNETEGQSQ+YLN+LAEKIN +L T+E++LN IR  QEEIDLYRWLV
Sbjct: 241 HIIVAARIEISSYLNETEGQSQKYLNELAEKINSILDTLENHLNTIRIDQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
            HI+N PT +TLV+PKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 DHIDNFPTELTLVVPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYF------REEEARKIYEYLSSLMKWYIVPYTT 420
           IRAVDLVYNEVRKEDKYKIVWIPV+P++        E+EARK YEYLSSLMKWYIVPYTT
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVVPEHSLEHSQEEEKEARKKYEYLSSLMKWYIVPYTT 420

Query: 421 KIAGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN 480
           KIAGWRYLE+ WQ RQDPLVVVMNS+S+VEF NAIHLIRVWG EAIPFT  RT ALL+KN
Sbjct: 421 KIAGWRYLEENWQLRQDPLVVVMNSQSRVEFTNAIHLIRVWGTEAIPFTNGRTHALLEKN 480

Query: 481 WPESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFE 540
           WPESTLLKFIE+PRLRSWVNQDRTIIFYGGKDP+WIQQFEEKVVEIKNDPY  EKG TFE
Sbjct: 481 WPESTLLKFIEQPRLRSWVNQDRTIIFYGGKDPNWIQQFEEKVVEIKNDPYIIEKGNTFE 540

Query: 541 IV-------EVNDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAV 600
           IV       EVNDFTLTP FWITQWGYFVIKS+IK SSA ETTEDILRLISY++ENGWAV
Sbjct: 541 IVRVGKKINEVNDFTLTPRFWITQWGYFVIKSRIKGSSAAETTEDILRLISYENENGWAV 600

Query: 601 LTVGSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQSLLYRPCERITLPQ 660
           L+VGSAPL+VGRG+LILGVLEDFN+WK TL   AF D FKD F   +L +  CER+TLP 
Sbjct: 601 LSVGSAPLLVGRGNLILGVLEDFNQWKRTLNIKAFPDAFKDYFNELNLKFHTCERMTLPG 660

Query: 661 FSGWIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           FSGWIP+ VNCPECP FM+ GIS KCN G  E
Sbjct: 661 FSGWIPMIVNCPECPRFMETGISFKCNHGRPE 692

BLAST of CmoCh08G010810 vs. ExPASy TrEMBL
Match: A0A6J1KK25 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111494729 PE=4 SV=1)

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 581/692 (83.96%), Postives = 615/692 (88.87%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNP+ASLHHPKLSTSTKDDQSI HLSDETVT +IYTKH EDDSV+IDVDNYIAL
Sbjct: 1   MSLSVPNNPIASLHHPKLSTSTKDDQSIRHLSDETVTGHIYTKHREDDSVRIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESII+TADRITETVTHGTEGRLIFSDDFLKVN VDPPLCTLHKVSSQLSCKAPG+EQAH
Sbjct: 61  VESIISTADRITETVTHGTEGRLIFSDDFLKVNVVDPPLCTLHKVSSQLSCKAPGIEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDIL SYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILVSYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVL+SPNSLIYSCLRAMKYITQLKNFSKYD  EL ELSSV RQIP  SYWII
Sbjct: 181 QLEGMKYRQVLLSPNSLIYSCLRAMKYITQLKNFSKYDTKELSELSSVLRQIPLVSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVA RIEISSYLNETEGQSQ+YLN+LAEKIN +L  +E +LN IR QQEEIDLYRWLV
Sbjct: 241 HIIVAARIEISSYLNETEGQSQKYLNELAEKINSILNMLEIHLNTIRIQQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
            HI+N PT ITLVLPKL+EGKVEAKPFIDGSTRLQVSVED LRDKNVILLISGLDIS+DD
Sbjct: 301 DHIDNFPTEITLVLPKLVEGKVEAKPFIDGSTRLQVSVEDGLRDKNVILLISGLDISEDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYF------REEEARKIYEYLSSLMKWYIVPYTT 420
           IRA+ LVYNEVRKEDKYKIVWIPVI ++        EEEARK YEYLSSLMKWYIVPYTT
Sbjct: 361 IRALHLVYNEVRKEDKYKIVWIPVIHEHSPEHSQEEEEEARKKYEYLSSLMKWYIVPYTT 420

Query: 421 KIAGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN 480
           KIAGWRYLE+ WQ RQDPLVVVMNS+S+VEF NAIHLIRVWG EAIPFT  RT ALL+KN
Sbjct: 421 KIAGWRYLEENWQLRQDPLVVVMNSQSRVEFTNAIHLIRVWGTEAIPFTNGRTHALLEKN 480

Query: 481 WPESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFE 540
           WPESTLLKFIE+PRLRSWVNQDR IIFYGGKDP+WIQQFEEKVVEIKNDPY K+KG TFE
Sbjct: 481 WPESTLLKFIEQPRLRSWVNQDRAIIFYGGKDPNWIQQFEEKVVEIKNDPYIKDKGNTFE 540

Query: 541 IV-------EVNDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAV 600
           IV       EVNDF LTP FWITQWGYFVIKSQ+K SSA ETTEDILRLISY++ENGWAV
Sbjct: 541 IVRVGKKINEVNDFALTPLFWITQWGYFVIKSQLKGSSAAETTEDILRLISYENENGWAV 600

Query: 601 LTVGSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQSLLYRPCERITLPQ 660
           L VGSAPL+VGRG+LILGVLEDFNKWK  L   AF D FKD F   +L +  CER+TLP 
Sbjct: 601 LAVGSAPLLVGRGNLILGVLEDFNKWKRNLNIEAFPDAFKDYFNELNLKFHTCERMTLPG 660

Query: 661 FSGWIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           FSGWIP+ VNCPECP FM+ GIS KCN G  E
Sbjct: 661 FSGWIPMIVNCPECPRFMETGISFKCNHGRPE 692

BLAST of CmoCh08G010810 vs. ExPASy TrEMBL
Match: A0A6J1CAZ1 (protein SIEVE ELEMENT OCCLUSION B-like OS=Momordica charantia OX=3673 GN=LOC111009572 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.6e-307
Identity = 525/691 (75.98%), Postives = 600/691 (86.83%), Query Frame = 0

Query: 1   MSLSVPNNPVAS-LHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIA 60
           MSL  P NP+A+ L HPKLST+TK+D S+ HLSDET+T +IYTKH EDD VKIDVDNY A
Sbjct: 1   MSLPHPTNPIAAPLVHPKLSTTTKEDPSLRHLSDETITGHIYTKHREDDRVKIDVDNYTA 60

Query: 61  LVESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQA 120
           LVESIITTADRITETV+ GTEGRLIFSDDFLKVNAVDPPLCTLH++SSQL+CKAPG+E+A
Sbjct: 61  LVESIITTADRITETVSQGTEGRLIFSDDFLKVNAVDPPLCTLHQISSQLACKAPGIEKA 120

Query: 121 HKTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLK 180
           H+TTL ILDIL SY WEAKAVLTLTAFA EYGDIWHLNHYS LDPLAKSL+MIKRVPLLK
Sbjct: 121 HQTTLYILDILVSYPWEAKAVLTLTAFATEYGDIWHLNHYSLLDPLAKSLAMIKRVPLLK 180

Query: 181 KQLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWI 240
           KQL+ +KYRQVL+SPNSLIYSCLRAM YI +LKNFSKYD+ EL ELSSV RQIP  SYWI
Sbjct: 181 KQLDSIKYRQVLLSPNSLIYSCLRAMNYINKLKNFSKYDIKELTELSSVLRQIPLVSYWI 240

Query: 241 IHIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWL 300
           IHIIVA+R EISSYLNETEGQSQ+YLN+LA+KI+ +L T+E++LN+I  QQ+EI LYRWL
Sbjct: 241 IHIIVASRTEISSYLNETEGQSQKYLNELADKIHSILNTLENHLNIITAQQDEIGLYRWL 300

Query: 301 VGHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDD 360
           V HI+N PT IT V+PKLIEGKV+AKPFIDGSTR QVS++DALR+KNVIL+ISGLDISDD
Sbjct: 301 VDHIDNFPTEITSVVPKLIEGKVDAKPFIDGSTRSQVSIQDALREKNVILVISGLDISDD 360

Query: 361 DIRAVDLVYNEVRKEDKYKIVWIPVIPDYF---REEEARKIYEYLSSLMKWYIVPYTTKI 420
           DIRA+ LVYNEV+KE+KYKIVWIP+IP++     EEEARK YEY+SS MKWYIVPYTTKI
Sbjct: 361 DIRALHLVYNEVKKEEKYKIVWIPIIPEHSVEDNEEEARKRYEYISSSMKWYIVPYTTKI 420

Query: 421 AGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWP 480
           AGWRYLE+ WQ RQDPLVVV++S+S++EF NAIHLIRVWG EAIPFT  RT  LL KNWP
Sbjct: 421 AGWRYLEENWQLRQDPLVVVLSSQSRIEFTNAIHLIRVWGTEAIPFTNGRTNTLLGKNWP 480

Query: 481 ESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIV 540
           ESTL KFI++PRL+SWVNQ+R+IIFYGGKDP+WIQQFEEKVVEIKNDP+ KEKG TFEIV
Sbjct: 481 ESTLFKFIDQPRLQSWVNQERSIIFYGGKDPNWIQQFEEKVVEIKNDPFIKEKGITFEIV 540

Query: 541 EV-------NDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLT 600
            V       NDFTL+P FWITQWGYFVIKSQ++ SSATETTEDILRLISY+++NGWAVL 
Sbjct: 541 RVGKNIKGQNDFTLSPRFWITQWGYFVIKSQLRGSSATETTEDILRLISYENDNGWAVLA 600

Query: 601 VGSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQ-SLLYRPCERITLPQF 660
           VGSAPL+V RG+L+LGV EDFNKWK  L   AF D F+D F  + +L +  CER+TLP F
Sbjct: 601 VGSAPLLVARGNLVLGVFEDFNKWKRNLNIKAFPDAFRDYFSNELNLKFHTCERMTLPGF 660

Query: 661 SGWIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           SGWIP+ VNCPECP FM+ GIS KC+ G  E
Sbjct: 661 SGWIPMIVNCPECPRFMETGISFKCSHGRPE 691

BLAST of CmoCh08G010810 vs. ExPASy TrEMBL
Match: A0A0A0LNQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193350 PE=4 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 8.3e-304
Identity = 517/689 (75.04%), Postives = 591/689 (85.78%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSL +PNNP+A    PKLS +TKDDQS+ H SDE VT +IYTKH ED+ +KIDVDNYIAL
Sbjct: 1   MSLPLPNNPLAPSVLPKLS-ATKDDQSLRHYSDEIVTSHIYTKHREDNRIKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETV  GTEGRLIFSD+FL VNAVDPPLCTLH VSSQLSCKAPG+E AH
Sbjct: 61  VESIITTADRITETVAQGTEGRLIFSDEFLNVNAVDPPLCTLHHVSSQLSCKAPGIETAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           +TTL+ILDIL SY WEAKAVLTLTAFA EYGDIWHLNHYS LDPLAKSL+MIKRVPLLKK
Sbjct: 121 ETTLEILDILVSYPWEAKAVLTLTAFATEYGDIWHLNHYSLLDPLAKSLAMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QL+ +KYRQ+L++PNSLIYSCL+AMKYI+ LKNFSKYD+ EL ELSSV RQIP  +YWII
Sbjct: 181 QLDSIKYRQLLLTPNSLIYSCLKAMKYISILKNFSKYDIKELSELSSVLRQIPLVAYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVA+RIEISSYLNETEGQSQ+Y+N+L+EKIN +L T+E++L +I+EQQ+EIDLYRWLV
Sbjct: 241 HIIVASRIEISSYLNETEGQSQKYMNELSEKINSILYTLENHLKIIKEQQDEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
            HI+N PT IT V+PKLIEGK +AKPFIDGST+LQVSVED LRDKNVIL+ISGLDIS+DD
Sbjct: 301 DHIDNFPTEITAVVPKLIEGKFDAKPFIDGSTKLQVSVEDGLRDKNVILVISGLDISEDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVI--PDYFREEEARKIYEYLSSLMKWYIVPYTTKIAG 420
           IRA+  +YNEV++EDKYKIVWIPVI       EEEARK YEY SSLMKWYIVPYT KIAG
Sbjct: 361 IRALHSIYNEVKREDKYKIVWIPVITVETEDEEEEARKKYEYASSLMKWYIVPYTRKIAG 420

Query: 421 WRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPES 480
           WRYLE+ WQ RQDPL+VVMNS+S+VEF NAIHLIRVWG +AIPFT  RT ALL KNWPES
Sbjct: 421 WRYLEENWQLRQDPLIVVMNSKSRVEFNNAIHLIRVWGIDAIPFTNGRTNALLAKNWPES 480

Query: 481 TLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEV 540
           TL KFI++PRL +WVNQ+R IIFYGGK+P WIQQFE+++VEIKNDPY KEKG TFEI+ V
Sbjct: 481 TLFKFIDQPRLMNWVNQERNIIFYGGKEPKWIQQFEDRIVEIKNDPYLKEKGNTFEIIRV 540

Query: 541 --------NDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTV 600
                   NDFTLTP FW+TQWGYFVIKSQ+K SSATETTEDILRLISY++ENGWA++ V
Sbjct: 541 GQNIKGDSNDFTLTPQFWLTQWGYFVIKSQLKGSSATETTEDILRLISYENENGWAIVAV 600

Query: 601 GSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQSLLYRPCERITLPQFSG 660
           GS PL+VGRG+LI+GVL+DFNKWK  +   AF D F+D F   +L +  CER+TLP FSG
Sbjct: 601 GSTPLLVGRGNLIMGVLQDFNKWKRNMNIKAFPDAFRDYFNELNLNFHICERMTLPGFSG 660

Query: 661 WIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           WIP+ VNCPECP FM+ GIS KCN G  E
Sbjct: 661 WIPMIVNCPECPRFMETGISFKCNHGRYE 688

BLAST of CmoCh08G010810 vs. NCBI nr
Match: XP_022930234.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV
Sbjct: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
           GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420
           IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420

Query: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480
           YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL
Sbjct: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480

Query: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540
           LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND
Sbjct: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540

Query: 541 FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600
           FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG
Sbjct: 541 FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600

Query: 601 DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP 660
           DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP
Sbjct: 601 DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP 660

Query: 661 LFMKVGISLKCNSGGRED 679
           LFMKVGISLKCNSGGRED
Sbjct: 661 LFMKVGISLKCNSGGRED 678

BLAST of CmoCh08G010810 vs. NCBI nr
Match: KAG6594041.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 631/678 (93.07%), Postives = 651/678 (96.02%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNPVASLHHPKLSTS KDDQS+ HLSD TVT +IYTKH EDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPVASLHHPKLSTSIKDDQSVRHLSDNTVTGHIYTKHREDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPG+EQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGIEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDIL SYSWEAKAVLTLTAFA EYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILVSYSWEAKAVLTLTAFATEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNF+KYDVDELPELSSV RQIPFFSYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFAKYDVDELPELSSVLRQIPFFSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVATRIEISSYLNETEGQSQRYLNDL EKIN LLCTIE +LNVIREQQE IDLYRWLV
Sbjct: 241 HIIVATRIEISSYLNETEGQSQRYLNDLTEKINHLLCTIEHHLNVIREQQEWIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
           GHI+NSPTRITLVL KLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 GHIHNSPTRITLVLAKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420
           IRAVDLVYNEVRKEDKYKIVWIPVIP+YFREEEARK YEYLSSLMKWYIVPYT KIAGWR
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPEYFREEEARKKYEYLSSLMKWYIVPYTRKIAGWR 420

Query: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480
           YLE+ WQFRQDPLVVVMNS+S+VEF NAIHLIRVWGAEAIPFTMDRTLALLKKNWPE TL
Sbjct: 421 YLEENWQFRQDPLVVVMNSQSRVEFRNAIHLIRVWGAEAIPFTMDRTLALLKKNWPEFTL 480

Query: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540
           LKFIEEPRLRSWVNQDRTIIFYGGKDP+WIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND
Sbjct: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPNWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVND 540

Query: 541 FTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600
           FTLTP FWITQWGYF+IKSQ+ +SS TETTEDILRLISYKDENGWAVLTVGSAPLVVGRG
Sbjct: 541 FTLTPRFWITQWGYFIIKSQLTNSSETETTEDILRLISYKDENGWAVLTVGSAPLVVGRG 600

Query: 601 DLILGVLEDFNKWKTTLTAFHDVFKDKFGLQSLLYRPCERITLPQFSGWIPIKVNCPECP 660
           +LILGVL+DFNKWKTTLTAFHDVFK+KFGLQSLL RPCERITLPQFSGWIP +VNCPECP
Sbjct: 601 NLILGVLKDFNKWKTTLTAFHDVFKEKFGLQSLLNRPCERITLPQFSGWIPTEVNCPECP 660

Query: 661 LFMKVGISLKCNSGGRED 679
             M++GIS KCN GGRED
Sbjct: 661 FVMEIGISFKCNGGGRED 678

BLAST of CmoCh08G010810 vs. NCBI nr
Match: KAG7026202.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 607/691 (87.84%), Postives = 630/691 (91.17%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNPVASLHHPKLSTSTKDDQSI HLSDETV  +IYTKH EDDSVKIDVDNY+AL
Sbjct: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSIRHLSDETVIGHIYTKHREDDSVKIDVDNYLAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITT DRITETVTHGTEGRLIFS DFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH
Sbjct: 61  VESIITTGDRITETVTHGTEGRLIFSGDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSM+KRVPLLKK
Sbjct: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMVKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQV++SPNSLIYSCLRAMKYITQLKNFSKYDV ELPELSSVRRQIPF SYWII
Sbjct: 181 QLEGMKYRQVVLSPNSLIYSCLRAMKYITQLKNFSKYDVKELPELSSVRRQIPFVSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVATRIEISSYLNETE QSQRYLNDLAEKINRLLCTIEDYLNVIREQQE IDLYRWLV
Sbjct: 241 HIIVATRIEISSYLNETESQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEWIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
           GHI+NSPTRITLVL KLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 GHIHNSPTRITLVLAKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWR 420
           IRAVDLVYNEVRKEDKYKIVWIPVIP+YFREEEARK YEYLSSLMKWYIVPYT KIAGWR
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPEYFREEEARKKYEYLSSLMKWYIVPYTRKIAGWR 420

Query: 421 YLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480
           YLE+ WQFRQDPLVVVM SESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL
Sbjct: 421 YLEENWQFRQDPLVVVMTSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPESTL 480

Query: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVE--- 540
           LKFIEEPRLRSWVNQDRTIIFYGGKDP+WIQQFE+KVVEIKNDPY KE G TFEIV    
Sbjct: 481 LKFIEEPRLRSWVNQDRTIIFYGGKDPNWIQQFEKKVVEIKNDPYIKETGNTFEIVRVGK 540

Query: 541 ----VNDFTLTPHFWITQWGYFVIKSQ----IKDSSATETTEDILRLISYKDENGWAVLT 600
               VNDF+LTP FWITQWGYFVIKSQ    +K  SATE   DI RLISYKDENGWA+L 
Sbjct: 541 KINGVNDFSLTPSFWITQWGYFVIKSQKESLLKSPSATEIRSDIFRLISYKDENGWAILA 600

Query: 601 VGSAPLVVGRGDLILGVLEDFNKWKTTL--TAFHDVFKDKFGLQSLLYRPCERITLPQFS 660
           VGSAPLVVGRG  ILGVLE+FN+WK TL   AF + FKD F      ++   RITLP+FS
Sbjct: 601 VGSAPLVVGRGPSILGVLEEFNQWKITLNKNAFPNAFKDYFDPFDFSFQTSGRITLPKFS 660

Query: 661 GWIPIKVNCPECPLFMKVGISLKCNSGGRED 679
           GWIP+KVNCPEC  FMK+GI+ +CN  GRED
Sbjct: 661 GWIPMKVNCPECHSFMKIGINFECNH-GRED 690

BLAST of CmoCh08G010810 vs. NCBI nr
Match: KAG6593861.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia] >KAG6594042.1 Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 585/692 (84.54%), Postives = 622/692 (89.88%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNP+ASLHHPKLSTSTKDDQS+ HLSD TVT +IYTKH EDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPIASLHHPKLSTSTKDDQSVRHLSDNTVTGHIYTKHREDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPG+EQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGIEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDIL SYSWEAKAVLTLTAFA EYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILVSYSWEAKAVLTLTAFATEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNF+KYD  EL  LSSV RQIP  SYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFAKYDTKELSVLSSVLRQIPLVSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVA RIEISSYLNETEGQSQ+YLN+LAEKIN +L T+E++LN IR +QEEIDLYRWLV
Sbjct: 241 HIIVAARIEISSYLNETEGQSQKYLNELAEKINSILDTLENHLNTIRIEQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
            HI+N PT +TLV+PKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISD+D
Sbjct: 301 DHIDNFPTELTLVVPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDED 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYF------REEEARKIYEYLSSLMKWYIVPYTT 420
           IRAVDLVYNEVRKEDKYKIVWIPV+P++        E+EARK YEYLSSLMKWYIVPYTT
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVVPEHSLEHSQEEEKEARKKYEYLSSLMKWYIVPYTT 420

Query: 421 KIAGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN 480
           KIAGWRYLE+ WQ RQDPLVVVMNS+S+VEF NAIHLIRVWG EAIPFT  RT ALL+KN
Sbjct: 421 KIAGWRYLEENWQLRQDPLVVVMNSQSRVEFRNAIHLIRVWGTEAIPFTNGRTHALLEKN 480

Query: 481 WPESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFE 540
           WPESTLLKFIE+PRLRSWVNQDRTIIFYGGKDP+WIQQFEEKVVEIKNDPY KEKG TFE
Sbjct: 481 WPESTLLKFIEQPRLRSWVNQDRTIIFYGGKDPNWIQQFEEKVVEIKNDPYIKEKGNTFE 540

Query: 541 IV-------EVNDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAV 600
           IV       EVND+TLTP FWITQWGYFVIKSQIK SSA ETTEDILRLISY++ENGWAV
Sbjct: 541 IVRVGKKINEVNDYTLTPRFWITQWGYFVIKSQIKGSSAAETTEDILRLISYENENGWAV 600

Query: 601 LTVGSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQSLLYRPCERITLPQ 660
           L+VGSAPL+VGRG+LILGVLEDFNKWK TL   AF D FKD F   +L +  CER+TLP 
Sbjct: 601 LSVGSAPLLVGRGNLILGVLEDFNKWKRTLNIKAFPDAFKDYFNELNLKFHTCERMTLPG 660

Query: 661 FSGWIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           FSGWIP+ VNCPECP FM+ GIS KCN G  E
Sbjct: 661 FSGWIPMIVNCPECPRFMETGISFKCNHGRPE 692

BLAST of CmoCh08G010810 vs. NCBI nr
Match: XP_023515205.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 584/692 (84.39%), Postives = 620/692 (89.60%), Query Frame = 0

Query: 1   MSLSVPNNPVASLHHPKLSTSTKDDQSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIAL 60
           MSLSVPNNP+ASLHHPKLSTSTKDDQS+ HLSD TVT +IYTKH EDDSVKIDVDNYIAL
Sbjct: 1   MSLSVPNNPIASLHHPKLSTSTKDDQSVRHLSDNTVTGHIYTKHREDDSVKIDVDNYIAL 60

Query: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAH 120
           VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPG+EQAH
Sbjct: 61  VESIITTADRITETVTHGTEGRLIFSDDFLKVNAVDPPLCTLHKVSSQLSCKAPGIEQAH 120

Query: 121 KTTLDILDILASYSWEAKAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180
           KTTLDILDIL SYSWEAKAVLTLTAFA EYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK
Sbjct: 121 KTTLDILDILVSYSWEAKAVLTLTAFATEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKK 180

Query: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWII 240
           QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNF+KYD  EL  LSSV RQIP  SYWII
Sbjct: 181 QLEGMKYRQVLVSPNSLIYSCLRAMKYITQLKNFAKYDTKELSVLSSVLRQIPLVSYWII 240

Query: 241 HIIVATRIEISSYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLV 300
           HIIVA RIEISSYLNETEGQSQ+YLN+LAEKIN +L T+E++LN IR +QEEIDLYRWLV
Sbjct: 241 HIIVAARIEISSYLNETEGQSQKYLNELAEKINSILDTLENHLNTIRIEQEEIDLYRWLV 300

Query: 301 GHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDD 360
            HI+N PT +TLV+PKLIEGKVEAKPFIDGST LQVSVEDALRDKNVILLISGLDISDDD
Sbjct: 301 DHIDNFPTELTLVVPKLIEGKVEAKPFIDGSTGLQVSVEDALRDKNVILLISGLDISDDD 360

Query: 361 IRAVDLVYNEVRKEDKYKIVWIPVIPDYF------REEEARKIYEYLSSLMKWYIVPYTT 420
           IRAVDLVYNEVRKEDKYKIVWIPV P++        E+EARK YEYLSSLMKWYIVPYTT
Sbjct: 361 IRAVDLVYNEVRKEDKYKIVWIPVAPEHSLEHSQEEEKEARKKYEYLSSLMKWYIVPYTT 420

Query: 421 KIAGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN 480
           KIAGWRYLE+ WQ RQDPLVVVMNS+S++EF NAIHLIRVWG EAIPFT  RT ALL+KN
Sbjct: 421 KIAGWRYLEENWQLRQDPLVVVMNSQSRIEFRNAIHLIRVWGTEAIPFTNGRTHALLEKN 480

Query: 481 WPESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFE 540
           WPESTLLKFIE+PRLRSWVNQDRTIIFYGGKDP+WIQQFEEKVVEIKNDPY KEKG TFE
Sbjct: 481 WPESTLLKFIEQPRLRSWVNQDRTIIFYGGKDPNWIQQFEEKVVEIKNDPYIKEKGNTFE 540

Query: 541 IV-------EVNDFTLTPHFWITQWGYFVIKSQIKDSSATETTEDILRLISYKDENGWAV 600
           IV       EVND+TLTP FWITQWGYFVIKSQIK SSA ETTEDILRLISY++ENGWAV
Sbjct: 541 IVRVGKKINEVNDYTLTPRFWITQWGYFVIKSQIKGSSAAETTEDILRLISYENENGWAV 600

Query: 601 LTVGSAPLVVGRGDLILGVLEDFNKWKTTLT--AFHDVFKDKFGLQSLLYRPCERITLPQ 660
           L+VGSAPL+VGRG+LILGVLEDFNKWK TL   AF D FKD F   +L +  CER+TLP 
Sbjct: 601 LSVGSAPLLVGRGNLILGVLEDFNKWKRTLNIKAFPDAFKDYFNELNLKFHTCERMTLPG 660

Query: 661 FSGWIPIKVNCPECPLFMKVGISLKCNSGGRE 678
           FSGWIP+ VNCPECP FM+ GIS KCN G  E
Sbjct: 661 FSGWIPMIVNCPECPRFMETGISFKCNHGRSE 692

BLAST of CmoCh08G010810 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 213.4 bits (542), Expect = 5.7e-55
Identity = 180/703 (25.60%), Postives = 315/703 (44.81%), Query Frame = 0

Query: 32  SDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHG-----TEGRLIFS 91
           SDE++   +  + H  D+ ++ V   ++LVE I+  A   +E          TE +L+ S
Sbjct: 38  SDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATLDSEDTNASMLPLPTEDKLMQS 97

Query: 92  DDFLKVNAVDPPLCTLHKVSSQLSCKAPGVEQAHKTTLDILDILASYSWEAKAVLTLTAF 151
                ++ +D     + +V+ +++ K+     +H+ T+ + + L+S+ W+ K VLTL AF
Sbjct: 98  S---MMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAF 157

Query: 152 AAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSLIYSCLRAMK 211
           A  YG+ W L  + S + LAKSL+M+K VP        ++ R  L S +  +   +R MK
Sbjct: 158 ALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--------VQNRVTLESVSQGLNDLIREMK 217

Query: 212 YIT----QLKNF-SKYDVDELPELSSVRRQIPFFSYWIIHIIVA--TRIEISSYLNETEG 271
            +T    +L     +Y   ++P+LS +   IP   YW I  ++A  ++I + + +     
Sbjct: 218 SVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITAMGHEMM 277

Query: 272 QSQRYLNDLAEKINRLLCTIEDYLNVIR------EQQEEIDLYRWLVGHINNSPTRITLV 331
            +Q  L + +   N+L    +     +R      E+Q   +  + L    + +      +
Sbjct: 278 NTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTTHIDNMKI 337

Query: 332 LPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDDIRAVDLVYNEVRK 391
           L  L+  K    P  DG T+ +V + D LR K V+LLIS L+I  D++   + +Y E R+
Sbjct: 338 LTALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLISDLNILQDELSIFEQIYTESRR 397

Query: 392 ---------EDKYKIVWIPV---IPDYFREEEARKIYEYLSSLMKWYIV--PYTTKIAGW 451
                       Y++VW+PV   I D+ R    +K +E L   M WY V  P   +    
Sbjct: 398 NLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHVV 457

Query: 452 RYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKNWPEST 511
            ++  +W F   P++VV++ +      NA+H+I +WG EA PFT  R   L ++      
Sbjct: 458 EFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSLN 517

Query: 512 LLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQF---------------EEKVVEIKNDP 571
           L+    +  + +W+  D  I  YGG D  WI++F               E   V  +N  
Sbjct: 518 LIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYVGKRNHS 577

Query: 572 YTKEKGYTFEIVEVNDFT-------LTPHFWITQWGYFVIKSQI-KDSSATETTEDILRL 631
           + ++     E++   + +       L   FW         K Q+ K     +  + I ++
Sbjct: 578 HREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVMQGIKKI 637

Query: 632 ISYKDENGWAVLTVGSAPLVVGRGDLILGVLEDFNKWKTTL------TAFHDVFKDKFGL 672
           +SY    GWA+L+ G   +++  G +   +      WKT +       A  D   D+   
Sbjct: 638 LSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMSDHHHDEVLR 697

BLAST of CmoCh08G010810 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 157.5 bits (397), Expect = 3.7e-38
Identity = 165/715 (23.08%), Postives = 306/715 (42.80%), Query Frame = 0

Query: 31  LSDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHGTEGRLIFSDDFL 90
           LSD+ V      K H  D +  DV + +++V  I  +   +    +   +  L+F  D+ 
Sbjct: 136 LSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKS--HVPSIDSSAPKPSLVFK-DYA 195

Query: 91  KVNAVDPPLCTLHKVSSQLSCKAPGVEQAH-------------KTTLDILDILASYSWEA 150
              + +     + ++S ++ CK     ++H              TT  +L +++ Y W+A
Sbjct: 196 DHTSFETFADLIDQISCEIDCKCLHGGESHGMMTSGLHLDSRNTTTFSVLSLVSKYRWDA 255

Query: 151 KAVLTLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSL 210
           K VL L+A A +YG    L    + + L KSL++IK++P +  +   +  R  L     L
Sbjct: 256 KLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLPSIFSRQNALHQR--LDKTRIL 315

Query: 211 IYSCLRAMKYITQLKNFSKYDVDELPE---LSSVRRQIPFFSYWIIHIIVATRIEIS--- 270
           +   +     I         D+ +LP     ++    IP   YWI+  ++     IS   
Sbjct: 316 MQDMVDLTTTI--------IDIYQLPPNHITAAFTDHIPTAVYWIVRCVLICVSHISGAS 375

Query: 271 -------------SYLNETEGQSQRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRW 330
                        S ++E   + ++    L E+  +   TIE+   +I E+ +E+     
Sbjct: 376 GFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIEE--GIIEEEYQELIQTFT 435

Query: 331 LVGHINNSPTRITLVLPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISD 390
            + H++  P  + L+ P      ++      G ++ +V + + L  K+V+LLIS L+  +
Sbjct: 436 TIIHVDVVPPLLRLLRP------IDFLYHGAGVSKRRVGI-NVLTQKHVLLLISDLENIE 495

Query: 391 DDIRAVDLVYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIV--PYTTKI 450
            ++  ++ +Y E  ++  ++I+W+PV  D++ E +  K +E L   M+WY++  P   + 
Sbjct: 496 KELYILESLYTEAWQQ-SFEILWVPV-QDFWTEADDAK-FEALHMNMRWYVLGEPRKLRR 555

Query: 451 AGWRYLEDKWQFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDRTLAL-LKKNW 510
           A  R++ + W F+  P++V ++ + +V   NA  ++ +W   A PFT  R   L  ++ W
Sbjct: 556 AAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHPFTTARERDLWSEQEW 615

Query: 511 PESTLLKFIEEPRLRSWVNQDRTIIFYGGKDPSWIQQFE--------------EKVVEIK 570
               L+   +   L   V+  + I  YGG+D  WI+ F               E V   K
Sbjct: 616 NLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQWIKNFTSLWRNVAKAANIQLEMVYVGK 675

Query: 571 NDPYTKEKGYTFEIVEVN------DFTLTPHFWITQWGYFVIKSQI------------KD 630
            +P    +     I E N      D      FW      +  K ++            K+
Sbjct: 676 RNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESMWESKQRMLKAHGIKGREGFKE 735

Query: 631 SSATETTEDILRLISYKDE-NGWAVLTVGSAPLVVGRGDLILGVLEDFNKW------KTT 672
                  ++++ ++ Y  E +GW +++  S  +V  +G+L    L +FN+W      K  
Sbjct: 736 EEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGNLFSRGLAEFNEWEVNIPTKGF 795

BLAST of CmoCh08G010810 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 96.3 bits (238), Expect = 1.0e-19
Identity = 120/598 (20.07%), Postives = 239/598 (39.97%), Query Frame = 0

Query: 26  QSISHLSDETVTDYIYTKHHEDDSVKIDVDNYIALVESIITTADRITETVTHGTEGRLIF 85
           + IS L+++ + + +  + H+ D   +D +  +  VE+I++        V      R + 
Sbjct: 5   RDISALNEDIIVEQL-LRSHDPDGRWLDSEMLLQEVETILS-------FVLQNDVSRPLL 64

Query: 86  SDDFLKVNAVDPPLCTL----HKVSSQLSCKAPGVEQAHKTTLDILDILASYSWEAKAVL 145
           +++ +    V     TL     ++S Q+ C   G  +  K T+ + D+L  Y W+AKAVL
Sbjct: 65  TENCITTIEVFDSKETLPYAIFRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAVL 124

Query: 146 TLTAFAAEYGDIWHLNHYSSLDPLAKSLSMIKRVPLLKKQLEGMKYRQVLVSPNSLIYSC 205
            L   AA YG +    H +  DP+A S++ + ++P     +E  K+R  L S N LI + 
Sbjct: 125 VLGVLAATYGGLLLPVHLAICDPVAASIAKLNQLP-----IERTKFRPWLESLNLLIKAM 184

Query: 206 LRAMKYITQLKNFSKYDVDELPELSSVRRQIPFFSYWIIHIIVATRIEISSYLNETEGQS 265
           +   K I + +                  +IPF                         Q+
Sbjct: 185 VDVTKCIIKFE------------------KIPF------------------------KQA 244

Query: 266 QRYLNDLAEKINRLLCTIEDYLNVIREQQEEIDLYRWLVGHINNSPTRITLVLPKLIEGK 325
           +   N L E ++ +      YL   R  +  +   + +                      
Sbjct: 245 KLDNNILGETLSNI------YLTTYRVVKSALTCMQQI---------------------- 304

Query: 326 VEAKPFIDGSTRLQVSVEDALRDKNVILLISGLDISDDDIRAVDLV--YNEVRKEDKYKI 385
               P+   + ++ ++    ++DK  +LL+S   +         L    +    E  Y+I
Sbjct: 305 ----PYFKQTQQISIT---EVQDKVTLLLLSKPPVEPLFFLLQQLYDHPSNTNTEQNYEI 364

Query: 386 VWIPVIPDYFREEEARKIYEYLSSLMKWYIV--PYTTKIAGWRYLEDKWQFR-QDPLVVV 445
           +W+P+       +E ++I+++ S+ + W  V  P+        + + +W ++  + ++VV
Sbjct: 365 IWVPIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDNEAMLVV 424

Query: 446 MNSESKVEFANAIHLIRVWGAEAIPFTMDRTLALLKKN-WPESTLLKFIEEPRLRSWVNQ 505
           ++S  +    NA+ ++ +WG +A PF++ R   L K++ W  + LL  I          +
Sbjct: 425 IDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHP------TFE 484

Query: 506 DRTIIFYGGKDPSWIQQFEEKVVEIKNDPYTKEKGYTFEIVEVNDF-------------- 565
            R I  +G ++  WI +F     +I+N       G+  E++ +++               
Sbjct: 485 GREICIFGSENLDWIDEFVSLARKIQN------LGFQLELIYLSNQRRDERAMEESSILF 500

Query: 566 --TLTPHFWITQWGYFVIKSQ---IKDSSATETTEDILRLI--SYKDENGWAVLTVGS 593
             TL   FW+        K +   I+ S      E++  L+   Y    GW ++  GS
Sbjct: 545 SPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIGNGS 500

BLAST of CmoCh08G010810 vs. TAIR 10
Match: AT1G60420.1 (DC1 domain-containing protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.5e-04
Identity = 28/100 (28.00%), Postives = 53/100 (53.00%), Query Frame = 0

Query: 367 VYNEVRKEDKYKIVWIPVIPDYFREEEARKIYEYLSSLMKWYIVPYTTKIAGWRYLEDKW 426
           VYNE+  +  ++IV++        +E+     +Y    M W  VP+T      R L++ +
Sbjct: 68  VYNELSSKVGFEIVFVS------GDEDEESFGDYFRK-MPWLAVPFTDSETRDR-LDELF 127

Query: 427 QFRQDPLVVVMNSESKVEFANAIHLIRVWGAEAIPFTMDR 467
           + R  P +V+++   K+   N + +IR +GA+A PFT ++
Sbjct: 128 KVRGIPNLVMVDDHGKLVNENGVGVIRSYGADAYPFTPEK 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SS878.0e-5425.60Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 S... [more]
Q93XX25.2e-3723.08Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 S... [more]
Q9FXE24.3e-2321.84Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 S... [more]
Match NameE-valueIdentityDescription
A0A6J1EQZ10.0e+00100.00protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1EX230.0e+0084.54protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KK250.0e+0083.96protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC1114947... [more]
A0A6J1CAZ11.6e-30775.98protein SIEVE ELEMENT OCCLUSION B-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0LNQ78.3e-30475.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193350 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022930234.10.0e+00100.00protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata][more]
KAG6594041.10.0e+0093.07Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7026202.10.0e+0087.84Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6593861.10.0e+0084.54Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023515205.10.0e+0084.39protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G01680.15.7e-5525.60CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640);... [more]
AT3G01670.13.7e-3823.08unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G67790.11.0e-1920.07unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G60420.13.5e-0428.00DC1 domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 265..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR33232:SF18SIEVE ELEMENT OCCLUSION-RELATEDcoord: 21..628
IPR027944Sieve element occlusion, C-terminalPFAMPF14577SEO_Ccoord: 482..672
e-value: 2.7E-21
score: 76.1
IPR027942Sieve element occlusion, N-terminalPFAMPF14576SEO_Ncoord: 32..298
e-value: 2.2E-50
score: 171.5
IPR039299Protein SIEVE ELEMENT OCCLUSIONPANTHERPTHR33232PROTEIN SIEVE ELEMENT OCCLUSION B-LIKEcoord: 21..628

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G010810.1CmoCh08G010810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010088 phloem development