CmoCh08G009810 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G009810
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionnon-specific phospholipase C3-like
LocationCmo_Chr08: 6386073 .. 6389648 (-)
RNA-Seq ExpressionCmoCh08G009810
SyntenyCmoCh08G009810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCCGGCTACCAGCGCCACCGCCACTGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAGATGAAAACCGATTCTCGAAACCTATCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATACACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCTTCTCAATCGAAATATCCTCTTCCGCGAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCGACGGTGATGAACGGATTCAAGCCGGAGGCGGTGCCGGTGTTCAAAGAGTTGGTGATGGAGTTCGGAGTGTGTGACCGGTGGTTCGCGTCGGTGCGGCGTCGACGCAGCCGAATCGACTGTTTGTGCACTCGGCGACGTCGTTTGGACTGAGTAGTAACGATACGAAGCAGTTGATCGGAGGACTTCCGCAGAAGACGATCTTCGAATCGTTGGAAGAAGAAGGCTTCAGTTTCGGGATTTACTACCAGCATCTTCCGTCTACTCTATTCTACCGGTACGTTTAATCGTTCATTATTCTCATATTTTTTTCAAATTATTGTTGTTTTTAATTACTTATTTTTATCTTTTCTTCAGAAGTAATTTGGACGTAATGTCTATAAAAATATTTGAAATAAAATATATATATACACACATCACTATCATTACTGTATTTGATGCAATATATTGGGGAATAAAAAAAAGATGGTGTTGGAGAATTCGTTTAAAGGTCAGGAGTATTTTTATTGGACAACTTTACCCCCATAATTTTATTAATATATATATATATATATACATATATCAAAATGCAATACAAGTAAAAAGTTTTAGAACGGAGAGCTAATAAAAGATATTCAGCGATTTGATCATTTGGTTTTGAAATTAGGTTTGTATTTTTTTATTTAATTCCTCAGATTTTTATATATAATTTTAAAAAAAAACATTTAAATTCTTAATTATATTTCAATAAAAATGAAGAATTACCTTTTTTCGGTTTTTAAAATTTTTCTTGGGTAGAAGAGAATATTTTTTTTTTAATAATAAAGTTATGTGGTTTTTCATAATTAGCTCTCAAGTTGAATCTTTAGAGGGGTGTTTCATTGCCACCATGCACAAAGCATATTTACTCATTACAATTCATAAAATAAATCTAAATGTACTGTTAGAAAAAAAAAAAATTTATCTCAAGTTTGATATATATATATATATATATATATATATATATTTATTTATTTATTTATTTATTGTTAAGGTTGGGTTGAATTTTTAATAAAATCGAAAATTTGGATCAATTCGTGGGTAGACATTTTTTTGTCGGATCAACCCGATCAATCCAACTAACTCAAAAATAGAGTTACAATCCAAATAAACTTAGGATTAAGAATATTTAACGTTTGTAATCAATATTTGTGATTAGTGATGTACGTTAATATTCAATGATATTTGAATTTTATGAAATTTTAATTATTAATATAAAGTTAGAATTTGTTTGAGTTATAGAATTTTAATTATTAATTTTAATTTTAATTATTTGGATAGATGGAAATCAAGGTATCAATCAATTATAGAATAATTTCAACAAAATAAATTGATTTGTTTTTCTTTGTTAGGAATCTAAGGAAACTGAAATACATAAAGAATTTTCATCCCTTTGGGATTGATTTCAAGAGACACTGTGAAGAAGGAAAGCTCCCAAACTACGTAGTGATAGAACAGAGATTCTTCGATTTAGCATCATTACCTGGAAACGACGATCATCCTTCTCACGACGTTGCAGAAGGCCAAAAACTCATCAAAGAAGTCTACGAAGCTTTGCGATCCAGTCCACAGTGGAATCAGATCCTGTTCTTGATCACCTACGATGAGCATGGAGGTTTCTTCGACCACGTCCCCACGCCGCTCGTCGGAGTCCCTAATCCCGACGGCCGCGTCGGTCCTCCTCCCTACAATTTCATGTTCGATCGCCTCGGAGTTAGGGTTCCGACGGTTTTTGTTTCTCCTTGGATTCAACCCGGAACAGGTAATCTTTTGCTTTCTGAATTTTGATTGATTCCTGGTTCTGTTATTTTATAGGATTTTTGGTGTTTGATATTTTGATTCCTAATTAATGACGGAGATAGTATGTGACATGATGCAGTGTTGCACAGGCCGTTGGGGCCAGATCCGTAATCGGAGTTCGAGCATTCGTCAATTCCTGCGACGGTGAAGAAGATTTTTGGTCTCGAGCAAATCTTGACGAAGCGCGACGAATGGGCCGGCACTTTTGAGATTGTTCTTAATCGACACAGCCCAAGAACTGATTGTCCAGGTCTGATTTCTCTTTCTTGATTGTTCCATTAATTCTTAATATCTATAATTGCATTGTCAGGAAGTCTACACCTCCATTAGTATCGAGTCTTTTAGGAAAACCAAAAGCAAACTTATGCTCAAAGTGAACAATATCATATCATTGTTGAGAGTCGTGGTTTCTAACATGGTATCAGAGTCATGCTCTTAACTTAACCATGTCAATAGAATTCTCAAATGTCGAACAAAGAAGTTGCGAGCCTCGTTTGTGTATCAAACAAAGGATGTACTTTGTTCGAGGACTCCAGAGAAGGAGTCGAATCTCTACTAAGGGGAGGTTGTTTGAGGGCTCCATAGGCCTTGGGAGAGGATCTATAGTGTATTTTGTTCGAGGGGAGGATTGTTGGGAGAAAATCCCGCGTTAGCTAACTTAGGGAAGGATCATGGGTTTATAACTAAGGAATACATATCCATTGATACGAGGCTTTTTGGGAAACCAAAAGCAAACCCATGAGAACTTATACTCAAAGTGGACAATATCATACTATCGTGGAGAGTCGCGGTTCCTAACATCATATCTATGGTCTGATTTGTCTGATTTCTCTTTCTTGATTATTGTTCAATTCTTAATATCCATAATCATATCCATGATTGGATTTCTTTTTGGATCGTTGGTTTAAGAACATGTAAACATATTCATGTTCAATATAAATCATCCTAATCCACCAACCAATATTACTCTGACTAATACAACAGTGAAGCTAAAGGATCCAGTGAAATTACGAGACGTGGAGGCGAACGAGACGAGGCGACTGAGCGAGTTCCAGGAGGAGTTGGTACAGTTAGCAGCAGTGCTGAAAGGAGATGAGAAGAAGGAAATAAATCTGGAGAAGGTAGCAGAGAAGATGAGAGTTGTTGAGGGAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCCTAAAAGAATGTGAGAAAGCCATGGAAAATGGAGGTGATGAGTGTGAGATTGTTGTTTGTGGAGAGAACCAACAAGTTCTTCAGCCTTCTTCCAAGTCTTTTGCTCGCAGGTTCCTTGCCTGCTTGGCTTGCCATGGTTGATTCAGTATTAAAATCATCTGATCTTGTTTATGCACCTTCAGTTTGA

mRNA sequence

ATGTCTCCGGCTACCAGCGCCACCGCCACTGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAGATGAAAACCGATTCTCGAAACCTATCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATACACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCTTCTCAATCGAAATATCCTCTTCCGCGAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCGACGGTGATGAACGGATTCAAGCCGGAGGCGGTGCCGGTGTTCAAAGAGTTGTTGATCGGAGGACTTCCGCAGAAGACGATCTTCGAATCGTTGGAAGAAGAAGGCTTCAGTTTCGGGATTTACTACCAGCATCTTCCGTCTACTCTATTCTACCGGAATCTAAGGAAACTGAAATACATAAAGAATTTTCATCCCTTTGGGATTGATTTCAAGAGACACTGTGAAGAAGGAAAGCTCCCAAACTACGTAGTGATAGAACAGAGATTCTTCGATTTAGCATCATTACCTGGAAACGACGATCATCCTTCTCACGACGTTGCAGAAGGCCAAAAACTCATCAAAGAAGTCTACGAAGCTTTGCGATCCAGTCCACAGTGGAATCAGATCCTGTTCTTGATCACCTACGATGAGCATGGAGGTTTCTTCGACCACGTCCCCACGCCGCTCGTCGGAGTCCCTAATCCCGACGGCCGCGTCGGTCCTCCTCCCTACAATTTCATGTTCGATCGCCTCGGAGTTAGGGTTCCGACGGTTTTTGTTTCTCCTTGGATTCAACCCGGAACAGTGAAGCTAAAGGATCCAGTGAAATTACGAGACGTGGAGGCGAACGAGACGAGGCGACTGAGCGAGTTCCAGGAGGAGTTGGTACAGTTAGCAGCAGTGCTGAAAGGAGATGAGAAGAAGGAAATAAATCTGGAGAAGGTAGCAGAGAAGATGAGAGTTGTTGAGGGAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCCTAAAAGAATGTGAGAAAGCCATGGAAAATGGAGGTTCCTTGCCTGCTTGGCTTGCCATGGTTGATTCAGTATTAAAATCATCTGATCTTGTTTATGCACCTTCAGTTTGA

Coding sequence (CDS)

ATGTCTCCGGCTACCAGCGCCACCGCCACTGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAGATGAAAACCGATTCTCGAAACCTATCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATACACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCTTCTCAATCGAAATATCCTCTTCCGCGAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCGACGGTGATGAACGGATTCAAGCCGGAGGCGGTGCCGGTGTTCAAAGAGTTGTTGATCGGAGGACTTCCGCAGAAGACGATCTTCGAATCGTTGGAAGAAGAAGGCTTCAGTTTCGGGATTTACTACCAGCATCTTCCGTCTACTCTATTCTACCGGAATCTAAGGAAACTGAAATACATAAAGAATTTTCATCCCTTTGGGATTGATTTCAAGAGACACTGTGAAGAAGGAAAGCTCCCAAACTACGTAGTGATAGAACAGAGATTCTTCGATTTAGCATCATTACCTGGAAACGACGATCATCCTTCTCACGACGTTGCAGAAGGCCAAAAACTCATCAAAGAAGTCTACGAAGCTTTGCGATCCAGTCCACAGTGGAATCAGATCCTGTTCTTGATCACCTACGATGAGCATGGAGGTTTCTTCGACCACGTCCCCACGCCGCTCGTCGGAGTCCCTAATCCCGACGGCCGCGTCGGTCCTCCTCCCTACAATTTCATGTTCGATCGCCTCGGAGTTAGGGTTCCGACGGTTTTTGTTTCTCCTTGGATTCAACCCGGAACAGTGAAGCTAAAGGATCCAGTGAAATTACGAGACGTGGAGGCGAACGAGACGAGGCGACTGAGCGAGTTCCAGGAGGAGTTGGTACAGTTAGCAGCAGTGCTGAAAGGAGATGAGAAGAAGGAAATAAATCTGGAGAAGGTAGCAGAGAAGATGAGAGTTGTTGAGGGAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCCTAAAAGAATGTGAGAAAGCCATGGAAAATGGAGGTTCCTTGCCTGCTTGGCTTGCCATGGTTGATTCAGTATTAAAATCATCTGATCTTGTTTATGCACCTTCAGTTTGA

Protein sequence

MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVMNGFKPEAVPVFKELLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTVKLKDPVKLRDVEANETRRLSEFQEELVQLAAVLKGDEKKEINLEKVAEKMRVVEGASYCENALKSFLKECEKAMENGGSLPAWLAMVDSVLKSSDLVYAPSV
Homology
BLAST of CmoCh08G009810 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.3e-136
Identity = 258/484 (53.31%), Postives = 305/484 (63.02%), Query Frame = 0

Query: 10  TGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPS 69
           T  G S + PIKTIV+LVQENRSFDH LGW K LN EIDGVT  +  S  +S+SD NS  
Sbjct: 4   TTKGGSGSYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLR 63

Query: 70  IFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMS 129
           + FG+ S YV+PDPGHSIQDI+EQ+F +PW        P     M GFAQNAER +KGMS
Sbjct: 64  VVFGDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNP-GHPNMSGFAQNAERNKKGMS 123

Query: 130 ATVMNGFKPEAVPVFKE----------------------------------------LLI 189
           + VMNGFKP A+PV+KE                                        LL+
Sbjct: 124 SAVMNGFKPNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLL 183

Query: 190 GGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKL 249
            G PQKTIFESL+E GFSFGIYYQ  PSTLFYRNLRKLKY+ +FH +GI FK+ C+EGKL
Sbjct: 184 EGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKL 243

Query: 250 PNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHG 309
           PNYVV+EQR+FDL S P NDDHPSHDV+EGQKL+KEVYEALRSSPQWN+ILF+ITYDEHG
Sbjct: 244 PNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHG 303

Query: 310 GFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV---------- 369
           GF+DHVPTP+ GVPNPDG +GPPPYNF F+RLGVRVPT F+SPWI+PGTV          
Sbjct: 304 GFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPR 363

Query: 370 ----------------KLKD-------------------------------PVKLRDVEA 397
                           KLKD                               P+KLR   A
Sbjct: 364 SQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETLSTPIKLRGTMA 423

BLAST of CmoCh08G009810 vs. ExPASy Swiss-Prot
Match: Q9S816 (Non-specific phospholipase C5 OS=Arabidopsis thaliana OX=3702 GN=NPC5 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 5.4e-133
Identity = 254/480 (52.92%), Postives = 301/480 (62.71%), Query Frame = 0

Query: 15  STASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGN 74
           S + PIKTIV+LVQENRSFDH LGW K LN EIDGV   ++   P  +SD NS ++ FG+
Sbjct: 8   SESYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGD 67

Query: 75  ASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVMN 134
            S YVDP+PGHSI+DI+EQ+F +PW        P P ATM GFAQNAER  KGMS+ VMN
Sbjct: 68  QSQYVDPNPGHSIRDIYEQVFGKPWDSGHPDPNPGP-ATMSGFAQNAERKMKGMSSAVMN 127

Query: 135 GFKPEAVPVFKE----------------------------------------LLIGGLPQ 194
           GFKP+A+PV+KE                                        LLI G PQ
Sbjct: 128 GFKPDALPVYKELVQNFAICDRWFASVPGATQPNRLFIHSATSHGTTNNERKLLIEGFPQ 187

Query: 195 KTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVV 254
           KTIFESL+E GF+FGIYYQ  P+TLFYRNLRKLKY+  FH +G+ FK+ C+EG LPNYVV
Sbjct: 188 KTIFESLDEAGFTFGIYYQCFPTTLFYRNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVV 247

Query: 255 IEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDH 314
           +EQR++DL   P NDDHPSHDV+EGQKL+KEVYEALRSSPQWN+ILF+ITYDEHGGF+DH
Sbjct: 248 VEQRWYDLLLNPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDH 307

Query: 315 VPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV--------------- 374
           VPTPL GVPNPDG +GPPPYNF F+RLGVRVPT F+SPWI+PGTV               
Sbjct: 308 VPTPLDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEH 367

Query: 375 -----------KLKD-------------------------------PVKLRDVEANETRR 398
                      KLKD                               PVK+R   A E   
Sbjct: 368 SSIPATVKKIFKLKDFLTKRDSWAGTFESVITRNSPRQDCPETLSNPVKMRGTVAKENAE 427

BLAST of CmoCh08G009810 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.5e-130
Identity = 252/490 (51.43%), Postives = 305/490 (62.24%), Query Frame = 0

Query: 8   TATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNS 67
           T++G G S+ASPIKTIV+LVQENRSFDHMLGW K LNPEIDGV++    S P+STSDPNS
Sbjct: 5   TSSGGG-SSASPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNS 64

Query: 68  PSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKG 127
             IFFG  S  +DPDPGHS Q I+EQ+F +P+SD  +S YP P+  M GF QNAE I KG
Sbjct: 65  AQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSD--ESPYPDPK--MNGFVQNAEAITKG 124

Query: 128 MS-ATVMNGFKPEAVPVFKEL--------------------------------------- 187
           MS   VM GF PE +PVFKEL                                       
Sbjct: 125 MSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTN 184

Query: 188 -LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 247
            L+ G PQ+T+FESLEE GF+FGIYYQ  P+ LFYRN+RKLKY+ NFH + + FKRHC+E
Sbjct: 185 TLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKE 244

Query: 248 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 307
           GKLPNYVVIE R+F + S P NDDHP +DV EGQ L+KE+YEALR+SPQWN+ILF++ YD
Sbjct: 245 GKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYD 304

Query: 308 EHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV------- 367
           EHGG++DHVPTP++GVPNPDG VGP PYNF FDRLGVRVP + +SPWI+PGTV       
Sbjct: 305 EHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGP 364

Query: 368 -------------------KLKD-------------------------PVKL------RD 397
                               LK                          PV L      RD
Sbjct: 365 EPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTLPELPRARD 424

BLAST of CmoCh08G009810 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 7.6e-111
Identity = 224/483 (46.38%), Postives = 280/483 (57.97%), Query Frame = 0

Query: 18  SPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGNASA 77
           SPIKTIV++V ENRSFDHMLGWMK LNPEI+GV  +   S P+S SDP+S  I FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGV--DGSESNPVSVSDPSSRKIKFGSGSH 84

Query: 78  YVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNA--ERIEKGMSATVMNG 137
           YVDPDPGHS Q I EQ+F    +D S    P     M GF Q A  E     MSA+VMNG
Sbjct: 85  YVDPDPGHSFQAIREQVFGS--NDTSMDPPP-----MNGFVQQAYSEDPSGNMSASVMNG 144

Query: 138 FKPEAVPVFKEL----------------------------------------LIGGLPQK 197
           F+P+ VPV+K L                                        L  G PQ+
Sbjct: 145 FEPDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQR 204

Query: 198 TIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVVI 257
           TIF++L++E FSFGIYYQ++P+ LFY++LRKLKY+  FH +G  FK H ++GKLP Y VI
Sbjct: 205 TIFDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVI 264

Query: 258 EQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDHV 317
           EQR+ D    P +DDHPSHDV +GQK IKEVYE LR+SPQWN+ L +ITYDEHGG+FDHV
Sbjct: 265 EQRYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHV 324

Query: 318 PTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV---------------- 377
           PTP+  VP+PDG VGP P+ F F+RLG+RVPT+ VSPWI+ GTV                
Sbjct: 325 PTPVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEH 384

Query: 378 --------------------------------------------KLKDPVKLRDVEANET 399
                                                        L +PVK+R  EANE 
Sbjct: 385 SSIPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEK 444

BLAST of CmoCh08G009810 vs. ExPASy Swiss-Prot
Match: Q8L7Y9 (Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.2e-105
Identity = 212/478 (44.35%), Postives = 274/478 (57.32%), Query Frame = 0

Query: 19  PIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGNASAY 78
           PIKTIV++V ENRSFDH+LGW+KS  PEIDG+T +   S P++ SDPNS  IF  + + +
Sbjct: 37  PIKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKE--SNPLNVSDPNSKKIFVSDDAVF 96

Query: 79  VDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVMNGFKP 138
           VD DPGHS Q I EQIF    +D S          M GFAQ +E +E GM+  VM+GFKP
Sbjct: 97  VDMDPGHSFQAIREQIFGS--NDTSGD------PKMNGFAQQSESMEPGMAKNVMSGFKP 156

Query: 139 EAVPVFKEL----------------------------------------LIGGLPQKTIF 198
           E +PV+ EL                                        L+ G PQKTIF
Sbjct: 157 EVLPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIF 216

Query: 199 ESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVVIEQR 258
           +SL+E G SFGIYYQ++P+T F+++LR+LK++  FH + + FK   + GKLPNY V+EQR
Sbjct: 217 DSLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQR 276

Query: 259 FFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDHVPTP 318
           +FD+   P NDDHPSHDVA GQ+ +KEVYE LRSSPQW ++  LITYDEHGGF+DHVPTP
Sbjct: 277 YFDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTP 336

Query: 319 LVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV------------------- 378
           + GVPNPDG +GP P+ F FDRLGVRVPT  +SPWI+ GTV                   
Sbjct: 337 VKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIP 396

Query: 379 ----------------------------KLKD------PVKLRDVE-------ANETRRL 397
                                       +++D      P KL +V+       A E  +L
Sbjct: 397 ATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKL 456

BLAST of CmoCh08G009810 vs. ExPASy TrEMBL
Match: A0A6J1HKJ6 (LOW QUALITY PROTEIN: non-specific phospholipase C4-like OS=Cucurbita moschata OX=3662 GN=LOC111464383 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.9e-216
Identity = 395/492 (80.28%), Postives = 395/492 (80.28%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI
Sbjct: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERIEKGMSATVMNGFKPEAVPVFKEL                                 
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVXASTQPNRLFVHSATSFGL 180

Query: 181 -------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240
                  LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF
Sbjct: 181 SSNDTKQLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240

Query: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300
           KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL
Sbjct: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300

Query: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-- 360
           FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT  
Sbjct: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTVL 360

Query: 361 -------------------------------------------------------VKLKD 396
                                                                  VKLKD
Sbjct: 361 HRPLGPDPXSEFEHSSIPATVKKIFGLEQILTKRDEWAGTFEIVLNRHSPRTDCPVKLKD 420

BLAST of CmoCh08G009810 vs. ExPASy TrEMBL
Match: A0A6J1KM42 (LOW QUALITY PROTEIN: non-specific phospholipase C3-like OS=Cucurbita maxima OX=3661 GN=LOC111494535 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.8e-208
Identity = 381/488 (78.07%), Postives = 387/488 (79.30%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           MSPA +ATA GDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVT+ENRFS P+
Sbjct: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDI+EQIFAEPWSDASQSK PLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERIEKGMSATVMN FKPEAVPVFKEL                                 
Sbjct: 121 AERIEKGMSATVMNXFKPEAVPVFKELVMEFGVCDRWFASVPXLFVHSATSFGLSSNDMK 180

Query: 181 -LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 240
            LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE
Sbjct: 181 QLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 240

Query: 241 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 300
           GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD
Sbjct: 241 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 300

Query: 301 EHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-------- 360
           EHGGFFDHVP PLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT        
Sbjct: 301 EHGGFFDHVPPPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTVLHRPLGS 360

Query: 361 -------------------------------------------------VKLKDPVKLRD 398
                                                            VKLKDPVKLRD
Sbjct: 361 DPSSEFEHSSIPATVKKIFGLEQFLTKRDEWAGTFEIVLNRHSPRTDCPVKLKDPVKLRD 420

BLAST of CmoCh08G009810 vs. ExPASy TrEMBL
Match: A0A6J1DR24 (non-specific phospholipase C3-like OS=Momordica charantia OX=3673 GN=LOC111022768 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.8e-171
Identity = 318/493 (64.50%), Postives = 350/493 (70.99%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           M+P    +  GD KS ++PIKTIV+LVQENRSFDHM+GWMK+LNPEIDGV+DE +FS PI
Sbjct: 1   MAPEIGGSDGGDAKSASNPIKTIVVLVQENRSFDHMIGWMKTLNPEIDGVSDETQFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPS+ FGNASA+VDPDPGHSIQDI EQIF +PWS A+ ++     ATMRGFAQN
Sbjct: 61  STSDPNSPSLHFGNASAFVDPDPGHSIQDIFEQIFGQPWSAAAAAQ--SSSATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERI KGMSATVMNGF+PEAVPVFKEL                                 
Sbjct: 121 AERIGKGMSATVMNGFRPEAVPVFKELVSEFGLCDRWFAAVPASTQPNRLYVHSATSFGL 180

Query: 181 -------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240
                  LIGGLPQKTIFESL+EEGFSFGIYYQ+LP+TLFYRNLRKLKY KNFH F IDF
Sbjct: 181 SSNDTKQLIGGLPQKTIFESLDEEGFSFGIYYQYLPTTLFYRNLRKLKYAKNFHQFDIDF 240

Query: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300
           K HC EGKLPNYVVIEQR+FDLAS+PGNDDHPSHDV+EGQK IK+VYEALRSSPQWN IL
Sbjct: 241 KSHCREGKLPNYVVIEQRYFDLASIPGNDDHPSHDVSEGQKFIKQVYEALRSSPQWNHIL 300

Query: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-- 360
           FLITYDEHGGFFDHVP PL GVPNPDG VGPPPYNF FDRLGVRVPT+F+SPWI+PGT  
Sbjct: 301 FLITYDEHGGFFDHVPPPLAGVPNPDGLVGPPPYNFNFDRLGVRVPTLFISPWIEPGTVL 360

Query: 361 -------------------------------------------------------VKLKD 397
                                                                  V L D
Sbjct: 361 HRAEGPDRTSEFEHSSIAATVKKIFGLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLND 420

BLAST of CmoCh08G009810 vs. ExPASy TrEMBL
Match: A0A1S3C4W0 (non-specific phospholipase C3-like OS=Cucumis melo OX=3656 GN=LOC103496908 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.4e-168
Identity = 321/519 (61.85%), Postives = 351/519 (67.63%), Query Frame = 0

Query: 14  KSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFG 73
           K +A PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVT++ +FS PISTS PNS SI FG
Sbjct: 7   KPSAYPIKTIVILVQENRSFDHMLGWMKTLNPQIDGVTNQTQFSNPISTSHPNSQSIPFG 66

Query: 74  NASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVM 133
           NASA+VDPDPGHSIQDI+EQ+FA PWSD     +PLP  TM+GFAQNAERI+KGMSATVM
Sbjct: 67  NASAFVDPDPGHSIQDIYEQVFAHPWSD---DLHPLPPPTMQGFAQNAERIQKGMSATVM 126

Query: 134 NGFKPEAVPVFKEL----------------------------------------LIGGLP 193
           NGFKPEAVPVFKEL                                        L+ G+P
Sbjct: 127 NGFKPEAVPVFKELVAEFGVCDRWFASVPASTQPNRLFLHSATSFGLTSNDTKQLMEGVP 186

Query: 194 QKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYV 253
           QKTIFESLEEEGFSFGIYYQ+ P+TLFYRNLRKLKYI NFH F +DFKR C EGKLPNYV
Sbjct: 187 QKTIFESLEEEGFSFGIYYQYFPATLFYRNLRKLKYIMNFHIFDLDFKRDCREGKLPNYV 246

Query: 254 VIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFD 313
           VIEQR+FDLASLPGNDDHPSHDV+EGQK IKEVYEALRSSPQWN+ILFLITYDEHGGFFD
Sbjct: 247 VIEQRYFDLASLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFD 306

Query: 314 HVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT--------------- 373
           HVP P  GVPNPD R+GPPPYNF FDRLGVRVPT+FVSPWI+PGT               
Sbjct: 307 HVPPPSAGVPNPDARLGPPPYNFNFDRLGVRVPTIFVSPWIEPGTVVHRPTGPDPTSEFE 366

Query: 374 ------------------------------------------VKLKDPVKLRDVEANETR 408
                                                     V L +PVKLR+VEAN+ R
Sbjct: 367 HSSIAATVKKIFGLKEFLTKRDQWAGTFDIVLNRHTPRTDCPVTLNNPVKLRNVEANDRR 426

BLAST of CmoCh08G009810 vs. ExPASy TrEMBL
Match: A0A5A7T833 (Non-specific phospholipase C3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold744G001040 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 3.1e-168
Identity = 313/480 (65.21%), Postives = 343/480 (71.46%), Query Frame = 0

Query: 14  KSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFG 73
           K +A PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVT++ +FS PISTS PNS SI FG
Sbjct: 7   KPSAYPIKTIVILVQENRSFDHMLGWMKTLNPQIDGVTNQTQFSNPISTSHPNSQSIPFG 66

Query: 74  NASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVM 133
           NASA+VDPDPGHSIQDI+EQ+FA PWSD     +PLP  TM+GFAQNAERI+KGMSATVM
Sbjct: 67  NASAFVDPDPGHSIQDIYEQVFAHPWSD---DLHPLPPPTMQGFAQNAERIQKGMSATVM 126

Query: 134 NGFKPEAVPVFKEL----------------------------------------LIGGLP 193
           NGFKPEAVPVFKEL                                        L+ G+P
Sbjct: 127 NGFKPEAVPVFKELVAEFGVCDRWFASVPASTQPNRLFLHSATSFGLTSNDTKQLMEGVP 186

Query: 194 QKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYV 253
           QKTIFESLEEEGFSFGIYYQ+ P+TLFYRNLRKLKYI NFH F +DFKR C EGKLPNYV
Sbjct: 187 QKTIFESLEEEGFSFGIYYQYFPATLFYRNLRKLKYIMNFHIFDLDFKRDCREGKLPNYV 246

Query: 254 VIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFD 313
           VIEQR+FDLASLPGNDDHPSHDV+EGQK IKEVYEALRSSPQWN+ILFLITYDEHGGFFD
Sbjct: 247 VIEQRYFDLASLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFD 306

Query: 314 HVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT--------------- 373
           HVP P  GVPNPD R+GPPPYNF FDRLGVRVPT+FVSPWI+PGT               
Sbjct: 307 HVPPPSAGVPNPDARLGPPPYNFNFDRLGVRVPTIFVSPWIEPGTVVHRPTGPDPTSEFE 366

Query: 374 ------------------------------------------VKLKDPVKLRDVEANETR 397
                                                     V L +PVKLR+VEAN+ R
Sbjct: 367 HSSIAATVKKIFGLKEFLTKRDQWAGTFDIVLNRHTPRTDCPVTLNNPVKLRNVEANDRR 426

BLAST of CmoCh08G009810 vs. NCBI nr
Match: XP_022964348.1 (LOW QUALITY PROTEIN: non-specific phospholipase C4-like [Cucurbita moschata])

HSP 1 Score: 759.6 bits (1960), Expect = 1.4e-215
Identity = 395/492 (80.28%), Postives = 395/492 (80.28%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI
Sbjct: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERIEKGMSATVMNGFKPEAVPVFKEL                                 
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVXASTQPNRLFVHSATSFGL 180

Query: 181 -------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240
                  LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF
Sbjct: 181 SSNDTKQLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240

Query: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300
           KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL
Sbjct: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300

Query: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-- 360
           FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT  
Sbjct: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTVL 360

Query: 361 -------------------------------------------------------VKLKD 396
                                                                  VKLKD
Sbjct: 361 HRPLGPDPXSEFEHSSIPATVKKIFGLEQILTKRDEWAGTFEIVLNRHSPRTDCPVKLKD 420

BLAST of CmoCh08G009810 vs. NCBI nr
Match: XP_023000253.1 (LOW QUALITY PROTEIN: non-specific phospholipase C3-like [Cucurbita maxima])

HSP 1 Score: 734.9 bits (1896), Expect = 3.8e-208
Identity = 381/488 (78.07%), Postives = 387/488 (79.30%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           MSPA +ATA GDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVT+ENRFS P+
Sbjct: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDI+EQIFAEPWSDASQSK PLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERIEKGMSATVMN FKPEAVPVFKEL                                 
Sbjct: 121 AERIEKGMSATVMNXFKPEAVPVFKELVMEFGVCDRWFASVPXLFVHSATSFGLSSNDMK 180

Query: 181 -LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 240
            LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE
Sbjct: 181 QLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 240

Query: 241 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 300
           GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD
Sbjct: 241 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 300

Query: 301 EHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-------- 360
           EHGGFFDHVP PLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT        
Sbjct: 301 EHGGFFDHVPPPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTVLHRPLGS 360

Query: 361 -------------------------------------------------VKLKDPVKLRD 398
                                                            VKLKDPVKLRD
Sbjct: 361 DPSSEFEHSSIPATVKKIFGLEQFLTKRDEWAGTFEIVLNRHSPRTDCPVKLKDPVKLRD 420

BLAST of CmoCh08G009810 vs. NCBI nr
Match: XP_038875468.1 (non-specific phospholipase C3-like [Benincasa hispida])

HSP 1 Score: 629.0 bits (1621), Expect = 2.9e-176
Identity = 328/494 (66.40%), Postives = 362/494 (73.28%), Query Frame = 0

Query: 1   MSPATSATATG-DGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKP 60
           MSP  + T  G DG+++A+PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVT++++FS P
Sbjct: 1   MSPEITPTGGGDDGEASANPIKTIVILVQENRSFDHMLGWMKTLNPQIDGVTNQSQFSNP 60

Query: 61  ISTSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQ 120
           ISTSDPNSPS+ FGNASA+VDPDPGHSIQDI+EQIFA+PWSD     +PLP  TM+GFAQ
Sbjct: 61  ISTSDPNSPSLPFGNASAFVDPDPGHSIQDIYEQIFAQPWSD---DLHPLPPPTMQGFAQ 120

Query: 121 NAERIEKGMSATVMNGFKPEAVPVFKEL-------------------------------- 180
           NAERI+KGMSATVMNGFKPEAVPVFKEL                                
Sbjct: 121 NAERIQKGMSATVMNGFKPEAVPVFKELVAEFGVCDRWFASVPASTQPNRLFVHSATSFG 180

Query: 181 --------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGID 240
                   LI GLPQKTIFESLEEEGFSFGIYYQ+LP+TLFYRNLRKLKYIKNFH F ID
Sbjct: 181 LTSNDTKQLIEGLPQKTIFESLEEEGFSFGIYYQYLPATLFYRNLRKLKYIKNFHVFDID 240

Query: 241 FKRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQI 300
           FKR C+EGKLPNYVVIEQR+FDLASLPGNDDHPSHDV+EGQK IK+VYEALRSSPQWN+I
Sbjct: 241 FKRDCQEGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKFIKQVYEALRSSPQWNEI 300

Query: 301 LFLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT- 360
           LF+ITYDEHGGFFDHVP P  GVPNPDGR+GPPP+NF FDRLGVRVPTVFVSPWI+PGT 
Sbjct: 301 LFVITYDEHGGFFDHVPPPSTGVPNPDGRLGPPPFNFKFDRLGVRVPTVFVSPWIEPGTV 360

Query: 361 --------------------------------------------------------VKLK 397
                                                                   V L 
Sbjct: 361 VHRPSGPDPTSEFEHSSIAATVKKIFSLKQFLTKRDQWAGTFDIVLNRHTARTDCPVTLN 420

BLAST of CmoCh08G009810 vs. NCBI nr
Match: KAG7026102.1 (Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 615.1 bits (1585), Expect = 4.3e-172
Identity = 340/494 (68.83%), Postives = 348/494 (70.45%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           MSPA SATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGV++ENRFS PI
Sbjct: 1   MSPAISATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVSNENRFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQD+HEQIFAEPWSDASQSK PLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDVHEQIFAEPWSDASQSKDPLPLATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERIEKGMSATVMNGFKPEAVPVFKEL                                 
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVPASTQPNRLFVHSATSFGL 180

Query: 181 -------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240
                  LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYR+  ++          I +
Sbjct: 181 SRNDTKQLIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRDRTEILRFSI-----ITW 240

Query: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300
           KR                              SHDV+EGQKLIKEVYEALRSSPQWNQIL
Sbjct: 241 KR----------------------------RSSHDVSEGQKLIKEVYEALRSSPQWNQIL 300

Query: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-- 360
           FLITYDEHGGFFDHVP PLVGVPNPDGRVGPPPYNFMFD LGVRVPTVFVSPWIQPGT  
Sbjct: 301 FLITYDEHGGFFDHVPPPLVGVPNPDGRVGPPPYNFMFDCLGVRVPTVFVSPWIQPGTVL 360

Query: 361 -------------------------------------------------------VKLKD 398
                                                                  VKLKD
Sbjct: 361 HRPLGPDPTSEFEHSSIPATVKKIFGLEQFLTKRDEWAATFEIVLNRHSPRTDCPVKLKD 420

BLAST of CmoCh08G009810 vs. NCBI nr
Match: XP_022155704.1 (non-specific phospholipase C3-like [Momordica charantia])

HSP 1 Score: 612.1 bits (1577), Expect = 3.7e-171
Identity = 318/493 (64.50%), Postives = 350/493 (70.99%), Query Frame = 0

Query: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60
           M+P    +  GD KS ++PIKTIV+LVQENRSFDHM+GWMK+LNPEIDGV+DE +FS PI
Sbjct: 1   MAPEIGGSDGGDAKSASNPIKTIVVLVQENRSFDHMIGWMKTLNPEIDGVSDETQFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120
           STSDPNSPS+ FGNASA+VDPDPGHSIQDI EQIF +PWS A+ ++     ATMRGFAQN
Sbjct: 61  STSDPNSPSLHFGNASAFVDPDPGHSIQDIFEQIFGQPWSAAAAAQ--SSSATMRGFAQN 120

Query: 121 AERIEKGMSATVMNGFKPEAVPVFKEL--------------------------------- 180
           AERI KGMSATVMNGF+PEAVPVFKEL                                 
Sbjct: 121 AERIGKGMSATVMNGFRPEAVPVFKELVSEFGLCDRWFAAVPASTQPNRLYVHSATSFGL 180

Query: 181 -------LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDF 240
                  LIGGLPQKTIFESL+EEGFSFGIYYQ+LP+TLFYRNLRKLKY KNFH F IDF
Sbjct: 181 SSNDTKQLIGGLPQKTIFESLDEEGFSFGIYYQYLPTTLFYRNLRKLKYAKNFHQFDIDF 240

Query: 241 KRHCEEGKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQIL 300
           K HC EGKLPNYVVIEQR+FDLAS+PGNDDHPSHDV+EGQK IK+VYEALRSSPQWN IL
Sbjct: 241 KSHCREGKLPNYVVIEQRYFDLASIPGNDDHPSHDVSEGQKFIKQVYEALRSSPQWNHIL 300

Query: 301 FLITYDEHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGT-- 360
           FLITYDEHGGFFDHVP PL GVPNPDG VGPPPYNF FDRLGVRVPT+F+SPWI+PGT  
Sbjct: 301 FLITYDEHGGFFDHVPPPLAGVPNPDGLVGPPPYNFNFDRLGVRVPTLFISPWIEPGTVL 360

Query: 361 -------------------------------------------------------VKLKD 397
                                                                  V L D
Sbjct: 361 HRAEGPDRTSEFEHSSIAATVKKIFGLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLND 420

BLAST of CmoCh08G009810 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-137
Identity = 258/484 (53.31%), Postives = 305/484 (63.02%), Query Frame = 0

Query: 10  TGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPS 69
           T  G S + PIKTIV+LVQENRSFDH LGW K LN EIDGVT  +  S  +S+SD NS  
Sbjct: 4   TTKGGSGSYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLR 63

Query: 70  IFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMS 129
           + FG+ S YV+PDPGHSIQDI+EQ+F +PW        P     M GFAQNAER +KGMS
Sbjct: 64  VVFGDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNP-GHPNMSGFAQNAERNKKGMS 123

Query: 130 ATVMNGFKPEAVPVFKE----------------------------------------LLI 189
           + VMNGFKP A+PV+KE                                        LL+
Sbjct: 124 SAVMNGFKPNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLL 183

Query: 190 GGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKL 249
            G PQKTIFESL+E GFSFGIYYQ  PSTLFYRNLRKLKY+ +FH +GI FK+ C+EGKL
Sbjct: 184 EGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKL 243

Query: 250 PNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHG 309
           PNYVV+EQR+FDL S P NDDHPSHDV+EGQKL+KEVYEALRSSPQWN+ILF+ITYDEHG
Sbjct: 244 PNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHG 303

Query: 310 GFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV---------- 369
           GF+DHVPTP+ GVPNPDG +GPPPYNF F+RLGVRVPT F+SPWI+PGTV          
Sbjct: 304 GFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPR 363

Query: 370 ----------------KLKD-------------------------------PVKLRDVEA 397
                           KLKD                               P+KLR   A
Sbjct: 364 SQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETLSTPIKLRGTMA 423

BLAST of CmoCh08G009810 vs. TAIR 10
Match: AT3G03540.1 (non-specific phospholipase C5 )

HSP 1 Score: 475.7 bits (1223), Expect = 3.8e-134
Identity = 254/480 (52.92%), Postives = 301/480 (62.71%), Query Frame = 0

Query: 15  STASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGN 74
           S + PIKTIV+LVQENRSFDH LGW K LN EIDGV   ++   P  +SD NS ++ FG+
Sbjct: 8   SESYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGD 67

Query: 75  ASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVMN 134
            S YVDP+PGHSI+DI+EQ+F +PW        P P ATM GFAQNAER  KGMS+ VMN
Sbjct: 68  QSQYVDPNPGHSIRDIYEQVFGKPWDSGHPDPNPGP-ATMSGFAQNAERKMKGMSSAVMN 127

Query: 135 GFKPEAVPVFKE----------------------------------------LLIGGLPQ 194
           GFKP+A+PV+KE                                        LLI G PQ
Sbjct: 128 GFKPDALPVYKELVQNFAICDRWFASVPGATQPNRLFIHSATSHGTTNNERKLLIEGFPQ 187

Query: 195 KTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVV 254
           KTIFESL+E GF+FGIYYQ  P+TLFYRNLRKLKY+  FH +G+ FK+ C+EG LPNYVV
Sbjct: 188 KTIFESLDEAGFTFGIYYQCFPTTLFYRNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVV 247

Query: 255 IEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDH 314
           +EQR++DL   P NDDHPSHDV+EGQKL+KEVYEALRSSPQWN+ILF+ITYDEHGGF+DH
Sbjct: 248 VEQRWYDLLLNPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDH 307

Query: 315 VPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV--------------- 374
           VPTPL GVPNPDG +GPPPYNF F+RLGVRVPT F+SPWI+PGTV               
Sbjct: 308 VPTPLDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEH 367

Query: 375 -----------KLKD-------------------------------PVKLRDVEANETRR 398
                      KLKD                               PVK+R   A E   
Sbjct: 368 SSIPATVKKIFKLKDFLTKRDSWAGTFESVITRNSPRQDCPETLSNPVKMRGTVAKENAE 427

BLAST of CmoCh08G009810 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 466.8 bits (1200), Expect = 1.8e-131
Identity = 252/490 (51.43%), Postives = 305/490 (62.24%), Query Frame = 0

Query: 8   TATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNS 67
           T++G G S+ASPIKTIV+LVQENRSFDHMLGW K LNPEIDGV++    S P+STSDPNS
Sbjct: 5   TSSGGG-SSASPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNS 64

Query: 68  PSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKG 127
             IFFG  S  +DPDPGHS Q I+EQ+F +P+SD  +S YP P+  M GF QNAE I KG
Sbjct: 65  AQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSD--ESPYPDPK--MNGFVQNAEAITKG 124

Query: 128 MS-ATVMNGFKPEAVPVFKEL--------------------------------------- 187
           MS   VM GF PE +PVFKEL                                       
Sbjct: 125 MSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTN 184

Query: 188 -LIGGLPQKTIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEE 247
            L+ G PQ+T+FESLEE GF+FGIYYQ  P+ LFYRN+RKLKY+ NFH + + FKRHC+E
Sbjct: 185 TLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKE 244

Query: 248 GKLPNYVVIEQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYD 307
           GKLPNYVVIE R+F + S P NDDHP +DV EGQ L+KE+YEALR+SPQWN+ILF++ YD
Sbjct: 245 GKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYD 304

Query: 308 EHGGFFDHVPTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV------- 367
           EHGG++DHVPTP++GVPNPDG VGP PYNF FDRLGVRVP + +SPWI+PGTV       
Sbjct: 305 EHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGP 364

Query: 368 -------------------KLKD-------------------------PVKL------RD 397
                               LK                          PV L      RD
Sbjct: 365 EPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTLPELPRARD 424

BLAST of CmoCh08G009810 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 402.1 bits (1032), Expect = 5.4e-112
Identity = 224/483 (46.38%), Postives = 280/483 (57.97%), Query Frame = 0

Query: 18  SPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGNASA 77
           SPIKTIV++V ENRSFDHMLGWMK LNPEI+GV  +   S P+S SDP+S  I FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGV--DGSESNPVSVSDPSSRKIKFGSGSH 84

Query: 78  YVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNA--ERIEKGMSATVMNG 137
           YVDPDPGHS Q I EQ+F    +D S    P     M GF Q A  E     MSA+VMNG
Sbjct: 85  YVDPDPGHSFQAIREQVFGS--NDTSMDPPP-----MNGFVQQAYSEDPSGNMSASVMNG 144

Query: 138 FKPEAVPVFKEL----------------------------------------LIGGLPQK 197
           F+P+ VPV+K L                                        L  G PQ+
Sbjct: 145 FEPDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQR 204

Query: 198 TIFESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVVI 257
           TIF++L++E FSFGIYYQ++P+ LFY++LRKLKY+  FH +G  FK H ++GKLP Y VI
Sbjct: 205 TIFDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVI 264

Query: 258 EQRFFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDHV 317
           EQR+ D    P +DDHPSHDV +GQK IKEVYE LR+SPQWN+ L +ITYDEHGG+FDHV
Sbjct: 265 EQRYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHV 324

Query: 318 PTPLVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV---------------- 377
           PTP+  VP+PDG VGP P+ F F+RLG+RVPT+ VSPWI+ GTV                
Sbjct: 325 PTPVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEH 384

Query: 378 --------------------------------------------KLKDPVKLRDVEANET 399
                                                        L +PVK+R  EANE 
Sbjct: 385 SSIPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEK 444

BLAST of CmoCh08G009810 vs. TAIR 10
Match: AT1G07230.1 (non-specific phospholipase C1 )

HSP 1 Score: 382.5 bits (981), Expect = 4.4e-106
Identity = 212/478 (44.35%), Postives = 274/478 (57.32%), Query Frame = 0

Query: 19  PIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPISTSDPNSPSIFFGNASAY 78
           PIKTIV++V ENRSFDH+LGW+KS  PEIDG+T +   S P++ SDPNS  IF  + + +
Sbjct: 37  PIKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKE--SNPLNVSDPNSKKIFVSDDAVF 96

Query: 79  VDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQNAERIEKGMSATVMNGFKP 138
           VD DPGHS Q I EQIF    +D S          M GFAQ +E +E GM+  VM+GFKP
Sbjct: 97  VDMDPGHSFQAIREQIFGS--NDTSGD------PKMNGFAQQSESMEPGMAKNVMSGFKP 156

Query: 139 EAVPVFKEL----------------------------------------LIGGLPQKTIF 198
           E +PV+ EL                                        L+ G PQKTIF
Sbjct: 157 EVLPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIF 216

Query: 199 ESLEEEGFSFGIYYQHLPSTLFYRNLRKLKYIKNFHPFGIDFKRHCEEGKLPNYVVIEQR 258
           +SL+E G SFGIYYQ++P+T F+++LR+LK++  FH + + FK   + GKLPNY V+EQR
Sbjct: 217 DSLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQR 276

Query: 259 FFDLASLPGNDDHPSHDVAEGQKLIKEVYEALRSSPQWNQILFLITYDEHGGFFDHVPTP 318
           +FD+   P NDDHPSHDVA GQ+ +KEVYE LRSSPQW ++  LITYDEHGGF+DHVPTP
Sbjct: 277 YFDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTP 336

Query: 319 LVGVPNPDGRVGPPPYNFMFDRLGVRVPTVFVSPWIQPGTV------------------- 378
           + GVPNPDG +GP P+ F FDRLGVRVPT  +SPWI+ GTV                   
Sbjct: 337 VKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIP 396

Query: 379 ----------------------------KLKD------PVKLRDVE-------ANETRRL 397
                                       +++D      P KL +V+       A E  +L
Sbjct: 397 ATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKL 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRQ72.3e-13653.31Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1[more]
Q9S8165.4e-13352.92Non-specific phospholipase C5 OS=Arabidopsis thaliana OX=3702 GN=NPC5 PE=1 SV=1[more]
Q9SRQ62.5e-13051.43Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1[more]
O810207.6e-11146.38Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1[more]
Q8L7Y96.2e-10544.35Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HKJ66.9e-21680.28LOW QUALITY PROTEIN: non-specific phospholipase C4-like OS=Cucurbita moschata OX... [more]
A0A6J1KM421.8e-20878.07LOW QUALITY PROTEIN: non-specific phospholipase C3-like OS=Cucurbita maxima OX=3... [more]
A0A6J1DR241.8e-17164.50non-specific phospholipase C3-like OS=Momordica charantia OX=3673 GN=LOC11102276... [more]
A0A1S3C4W02.4e-16861.85non-specific phospholipase C3-like OS=Cucumis melo OX=3656 GN=LOC103496908 PE=4 ... [more]
A0A5A7T8333.1e-16865.21Non-specific phospholipase C3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
XP_022964348.11.4e-21580.28LOW QUALITY PROTEIN: non-specific phospholipase C4-like [Cucurbita moschata][more]
XP_023000253.13.8e-20878.07LOW QUALITY PROTEIN: non-specific phospholipase C3-like [Cucurbita maxima][more]
XP_038875468.12.9e-17666.40non-specific phospholipase C3-like [Benincasa hispida][more]
KAG7026102.14.3e-17268.83Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022155704.13.7e-17164.50non-specific phospholipase C3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G03530.11.7e-13753.31non-specific phospholipase C4 [more]
AT3G03540.13.8e-13452.92non-specific phospholipase C5 [more]
AT3G03520.11.8e-13151.43non-specific phospholipase C3 [more]
AT2G26870.15.4e-11246.38non-specific phospholipase C2 [more]
AT1G07230.14.4e-10644.35non-specific phospholipase C1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 338..358
NoneNo IPR availablePANTHERPTHR31956:SF32NON-SPECIFIC PHOSPHOLIPASE C3coord: 319..398
coord: 148..319
coord: 13..148
IPR017850Alkaline-phosphatase-like, core domain superfamilyGENE3D3.40.720.10Alkaline Phosphatase, subunit Acoord: 11..150
e-value: 3.1E-17
score: 65.2
coord: 211..324
e-value: 2.3E-33
score: 118.2
IPR007312PhosphoesterasePFAMPF04185Phosphoesterasecoord: 20..147
e-value: 1.3E-21
score: 77.2
coord: 150..320
e-value: 1.6E-53
score: 182.2
IPR007312PhosphoesterasePANTHERPTHR31956NON-SPECIFIC PHOSPHOLIPASE C4-RELATEDcoord: 319..398
coord: 148..319
coord: 13..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G009810.1CmoCh08G009810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0003824 catalytic activity