CmoCh08G009710 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G009710
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionchaperone protein dnaJ 20, chloroplastic-like
LocationCmo_Chr08: 6303263 .. 6305106 (-)
RNA-Seq ExpressionCmoCh08G009710
SyntenyCmoCh08G009710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTAACGGGGGAAGCCAATTCAGACAAGAAATTCACAGGAATTTTTCTGCTTTTGCTCATTCCGTACTTACTTCCGCAGTCGACGAACCAGCGAGTGGTGAAGGTTCATCAGAGGAAGATTTCAGTGACATAATCGGAAGAAGAAATCACAGAGATGCGTGGTTACGGTTTGGCTTTATCTGGTACTGATTCACGTTGCTTTGTACCGGCGATGCCTTCGATTTTCTCTCCGAGAAGAGCCTCTCCGTCGGTAGGAAATTCGCAGGTTCTGTTTCCGAAATCGACACGGACTCCTTCTCTTTGCGTCAGAGCGAAGGCAGCCGTCGTCGACGAGGCGGTTGTCTCCGACGCTGTTGAGATTAGCTTCTACGATCTTTTAGGAATACCGGAGTCGGGGTCTATGGCGGAGATTAAGCAGGCGTACAAGCAGTTGGCTCTGAAATATCATCCGGACGTCTCGCCGCCGGATAGGATCGAGGAGTATACCAGAAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCTGATCCTAGTATGAGAGCTTTGTATGATAGAGACATGTTGGGAGGCCTTCACCTTGCTTTCTCTTCTCGAAGACGTGGCAACAACGAGGTACATTTCTTGCCGATTTTTGAGGTCAATTTGGTTTCTGAATCAATTAGGAATCGGATGCCTTCTCTACCTTATTTCATGGCAAATTATTTCGTTTTATTGAGTTGATTTTTGACCTGATTCGAACAATTTCGCGTCCTGATCCTGAATTAATTTCTATGACCTATTCGTTGATTCAACCTGGATTTCTGCAACCTCTCAATCGAGCTTTTCAATTGTTTATCCTACGCGTTCTGCTTGTACATGAATCGGCGTAGGGATTGCGGCTGCAAAAATCTTATGATTTGTTTTTGTATAGGTAATTAGATGAAGAACATAATCTGGTATTTCTTTAAAAAAAAAAAAAACTATTAGTTGAAACATAATTTACAAACTACAGAGGTATATTGACGATATACAAATGTTCTTTGAGGAATTTATTTTAGCAATTGATTAACCATAATTTTAGGATCTTTTGATTCTATCCTCTGTCTCACTTGCTAGTTGCTTAAGCTTGCCGCATGCTTCTATTATACTTTTCAAAATGCCAAAGTCACACAAGACGTTTTAGCGTTAAAATTCTTTACTTCACCATCGTTTCTTAAAATAATCTGTTCTTAATTCAAATGACTCAAGTGTTTCAAAACAGGGATAATCAAGACAAAATTATGATTCCTGGATATAATCATATGGCAGATATCAATTCTCGTAGTTTCTATATCAGAATTTCCTAAATTAAATATGCACTTGTCTGATTCTTTCCTTGAATTACTCAGGAAATGGAAGTGAAAAGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGGCTTGAGGAGAAGAAGCATGAACAAAGATTCAGGAGCAAGCATGTCGTGGGGAGCTCGAATGCGCAGGCAAAGGGACGAGCTTAACGACGACGACGACGACATGTAATTTGGTTTCTCCCTTGCTCTAAAACTTTCAACCTTCCTGCACATAGCTGATACAATATACAATAGCTGGATTCAAGAGCTTAATTAGCACTCTTTATACACAGCCTACTGAGTACTAGAACTTCTACTTCCACTATGAAACCCAAATTGTTTAGAGCCCAAAAATTGGGAAGTTTTTCATAGGGAATGTAAATCACTAGCAAACTAGTATTCAATGTATATATATATGGAAATTAAAATTTCTCGAGTACTTTGAGTTTCTAAACTGATCTCAGAATAATCTATCAGTGTTTACTTGATCATTCCTTGCTTGA

mRNA sequence

CCGTAACGGGGGAAGCCAATTCAGACAAGAAATTCACAGGAATTTTTCTGCTTTTGCTCATTCCGTACTTACTTCCGCAGTCGACGAACCAGCGAGTGGTGAAGGTTCATCAGAGGAAGATTTCAGTGACATAATCGGAAGAAGAAATCACAGAGATGCGTGGTTACGGTTTGGCTTTATCTGGTACTGATTCACGTTGCTTTGTACCGGCGATGCCTTCGATTTTCTCTCCGAGAAGAGCCTCTCCGTCGGTAGGAAATTCGCAGGTTCTGTTTCCGAAATCGACACGGACTCCTTCTCTTTGCGTCAGAGCGAAGGCAGCCGTCGTCGACGAGGCGGTTGTCTCCGACGCTGTTGAGATTAGCTTCTACGATCTTTTAGGAATACCGGAGTCGGGGTCTATGGCGGAGATTAAGCAGGCGTACAAGCAGTTGGCTCTGAAATATCATCCGGACGTCTCGCCGCCGGATAGGATCGAGGAGTATACCAGAAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCTGATCCTAGTATGAGAGCTTTGTATGATAGAGACATGTTGGGAGGCCTTCACCTTGCTTTCTCTTCTCGAAGACGTGGCAACAACGAGGAAATGGAAGTGAAAAGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGGCTTGAGGAGAAGAAGCATGAACAAAGATTCAGGAGCAAGCATGTCGTGGGGAGCTCGAATGCGCAGGCAAAGGGACGAGCTTAACGACGACGACGACGACATGTAATTTGGTTTCTCCCTTGCTCTAAAACTTTCAACCTTCCTGCACATAGCTGATACAATATACAATAGCTGGATTCAAGAGCTTAATTAGCACTCTTTATACACAGCCTACTGAGTACTAGAACTTCTACTTCCACTATGAAACCCAAATTGTTTAGAGCCCAAAAATTGGGAAGTTTTTCATAGGGAATGTAAATCACTAGCAAACTAGTATTCAATGTATATATATATGGAAATTAAAATTTCTCGAGTACTTTGAGTTTCTAAACTGATCTCAGAATAATCTATCAGTGTTTACTTGATCATTCCTTGCTTGA

Coding sequence (CDS)

ATGCGTGGTTACGGTTTGGCTTTATCTGGTACTGATTCACGTTGCTTTGTACCGGCGATGCCTTCGATTTTCTCTCCGAGAAGAGCCTCTCCGTCGGTAGGAAATTCGCAGGTTCTGTTTCCGAAATCGACACGGACTCCTTCTCTTTGCGTCAGAGCGAAGGCAGCCGTCGTCGACGAGGCGGTTGTCTCCGACGCTGTTGAGATTAGCTTCTACGATCTTTTAGGAATACCGGAGTCGGGGTCTATGGCGGAGATTAAGCAGGCGTACAAGCAGTTGGCTCTGAAATATCATCCGGACGTCTCGCCGCCGGATAGGATCGAGGAGTATACCAGAAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCTGATCCTAGTATGAGAGCTTTGTATGATAGAGACATGTTGGGAGGCCTTCACCTTGCTTTCTCTTCTCGAAGACGTGGCAACAACGAGGAAATGGAAGTGAAAAGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGGCTTGAGGAGAAGAAGCATGAACAAAGATTCAGGAGCAAGCATGTCGTGGGGAGCTCGAATGCGCAGGCAAAGGGACGAGCTTAACGACGACGACGACGACATGTAA

Protein sequence

MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDEAVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNKDSGASMSWGARMRRQRDELNDDDDDM
Homology
BLAST of CmoCh08G009710 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 9.6e-43
Identity = 93/157 (59.24%), Postives = 115/157 (73.25%), Query Frame = 0

Query: 44  TRTPSLCVRAKAAVVDEAVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSP 103
           TRT     R ++ +  +  V  + ++SFYDLLG+ ES ++ EIKQAYKQLA KYHPDVSP
Sbjct: 40  TRTRFSSTRIQSRLTHDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 99

Query: 104 PDRIEEYTRKFIKVQEAYETLSDPSMRALYDRDMLGGLHLAFSSRR--RGNNEEMEVKSG 163
           PDR+EEYT +FI+VQEAYETLSDP  R LYDRD+  G   +FS RR  R + E +E KS 
Sbjct: 100 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSE 159

Query: 164 WKSRWQDQLSGLRRRSMNKDSGASMSWGARMRRQRDE 199
           WK++WQ QLSGLRRRS  KD+  +MSW ARMRRQ+ E
Sbjct: 160 WKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQQQE 195

BLAST of CmoCh08G009710 vs. ExPASy Swiss-Prot
Match: Q9WZV3 (Chaperone protein DnaJ OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.1e-12
Identity = 32/65 (49.23%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 71  FYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMR 130
           +Y++LG+P   +  EIK+AYK+L  ++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 131 ALYDR 136
           A+YDR
Sbjct: 68  AMYDR 71

BLAST of CmoCh08G009710 vs. ExPASy Swiss-Prot
Match: A5IIT4 (Chaperone protein DnaJ OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1) OX=390874 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.1e-12
Identity = 32/65 (49.23%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 71  FYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMR 130
           +Y++LG+P   +  EIK+AYK+L  ++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 131 ALYDR 136
           A+YDR
Sbjct: 68  AMYDR 71

BLAST of CmoCh08G009710 vs. ExPASy Swiss-Prot
Match: B1LCI2 (Chaperone protein DnaJ OS=Thermotoga sp. (strain RQ2) OX=126740 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.1e-12
Identity = 32/65 (49.23%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 71  FYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMR 130
           +Y++LG+P   +  EIK+AYK+L  ++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 131 ALYDR 136
           A+YDR
Sbjct: 68  AMYDR 71

BLAST of CmoCh08G009710 vs. ExPASy Swiss-Prot
Match: Q05646 (Chaperone protein DnaJ OS=Erysipelothrix rhusiopathiae OX=1648 GN=dnaJ PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.7e-12
Identity = 36/65 (55.38%), Postives = 47/65 (72.31%), Query Frame = 0

Query: 71  FYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMR 130
           FY++LG+ +S + AEIK+AY+QLA KYHPD++  D  E    KF +VQEAYE LSD   R
Sbjct: 7   FYEILGVSKSATDAEIKKAYRQLAKKYHPDINKEDGAE---AKFKEVQEAYEVLSDSQKR 66

Query: 131 ALYDR 136
           A YD+
Sbjct: 67  ANYDQ 68

BLAST of CmoCh08G009710 vs. ExPASy TrEMBL
Match: A0A6J1HHM6 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111464407 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 7.3e-110
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDDDDDM
Sbjct: 181 DSGASMSWGARMRRQRDELNDDDDDM 206

BLAST of CmoCh08G009710 vs. ExPASy TrEMBL
Match: A0A6J1KFW2 (chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111494708 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.0e-104
Identity = 195/206 (94.66%), Postives = 202/206 (98.06%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMP IFSPRRASPSVGNSQVLFPKSTR PSLCVRAKAAVV+E
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASPSVGNSQVLFPKSTRNPSLCVRAKAAVVNE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSDAVEISFYDLLGIPESGSMAEIKQAYKQ+ALKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYDLLGIPESGSMAEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDP MRALYDRDMLGGLHLAFS+RRRGNNEEMEVKSGWKS WQDQLSGLRRRSMNK
Sbjct: 121 YETLSDPRMRALYDRDMLGGLHLAFSARRRGNNEEMEVKSGWKSLWQDQLSGLRRRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDD+D++
Sbjct: 181 DSGASMSWGARMRRQRDELNDDEDEV 206

BLAST of CmoCh08G009710 vs. ExPASy TrEMBL
Match: A0A6J1H3S2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460232 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.3e-86
Identity = 167/205 (81.46%), Postives = 183/205 (89.27%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MR  GLALSGTDSRC++PAMP IFSP RA P  GNSQVLFPK TR PS CVRAKA VV+E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSDAVEISFY+LLG+PESGSM EIKQAYKQ+A KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRG-NNEEMEVKSGWKSRWQDQLSGLRRRSMN 180
           YETLSDP MR LYDRDM  GLHLAFS+RRRG N+EEM+ K GWKSRWQDQLS L+RRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGASMSWGARMRRQRDELNDDDD 205
           K+S A+MSWGARMRRQRDEL++DD+
Sbjct: 181 KNSRANMSWGARMRRQRDELHNDDN 205

BLAST of CmoCh08G009710 vs. ExPASy TrEMBL
Match: A0A6J1KVG4 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499073 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.8e-85
Identity = 166/205 (80.98%), Postives = 180/205 (87.80%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MR  GLALSGTDSRC++PAMP IFSP RA P  GNSQVLFPK TR PS CVRAKA VV E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
            +VSDA+EISFY+LLG+PESGSM EIKQAYKQ+A KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRG-NNEEMEVKSGWKSRWQDQLSGLRRRSMN 180
           YETLSDP MR LYDRDM  GLHLAFS+RRRG N+EEME K GWKSRWQDQLS L+RRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGASMSWGARMRRQRDELNDDDD 205
           K+S A+MSWGARMRRQ DEL+DDD+
Sbjct: 181 KNSRANMSWGARMRRQGDELHDDDN 205

BLAST of CmoCh08G009710 vs. ExPASy TrEMBL
Match: A0A6J1CE65 (chaperone protein dnaJ 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111009883 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.9e-78
Identity = 155/198 (78.28%), Postives = 174/198 (87.88%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MR YGLALSGTDSRC++PAMP IFSPRRASP  GNSQV FPKS R PS  VRAK AVV+E
Sbjct: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPXEGNSQVSFPKSVRHPSASVRAK-AVVNE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
            +VS+AVE+SFY+LLG+PESGS+ EIKQAYKQ+A KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNN-EEMEVKSGWKSRWQDQLSGLRRRSMN 180
           YETLSDP  RALYDRDM  GLHLAFS+RRR +N E+ME KSGWK+RW+DQLS L+RRSM 
Sbjct: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180

Query: 181 KDSGASMSWGARMRRQRD 198
           KDS  +MSWGARMRRQ++
Sbjct: 181 KDSRENMSWGARMRRQKN 197

BLAST of CmoCh08G009710 vs. NCBI nr
Match: XP_022964372.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 406.4 bits (1043), Expect = 1.5e-109
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDDDDDM
Sbjct: 181 DSGASMSWGARMRRQRDELNDDDDDM 206

BLAST of CmoCh08G009710 vs. NCBI nr
Match: KAG6593757.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 199/206 (96.60%), Postives = 202/206 (98.06%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMP IFSPRRAS SVGNSQVLFPKSTRTPSLCVRAKAAVVDE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASSSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDPSMRALYDRDMLGGLHLAFS+RRRGNNEEMEVKSGWKSRWQDQLSGLR RSMNK
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSARRRGNNEEMEVKSGWKSRWQDQLSGLRTRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDD+DD+
Sbjct: 181 DSGASMSWGARMRRQRDELNDDEDDV 206

BLAST of CmoCh08G009710 vs. NCBI nr
Match: KAG7026089.1 (Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.8 bits (1000), Expect = 1.5e-104
Identity = 198/206 (96.12%), Postives = 201/206 (97.57%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMP IFSPRRAS SVGNSQVLFPKSTRTPSLCVRAKAAVVDE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASSSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLA KYHPDVSPP RIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLARKYHPDVSPPGRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDPSMRALYDRDMLGGLHLAFS+RRRGNNEEMEVKSGWKSRWQDQLSGLR RSMNK
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSARRRGNNEEMEVKSGWKSRWQDQLSGLRTRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDD+DD+
Sbjct: 181 DSGASMSWGARMRRQRDELNDDEDDV 206

BLAST of CmoCh08G009710 vs. NCBI nr
Match: XP_023000441.1 (chaperone protein dnaJ 20, chloroplastic [Cucurbita maxima])

HSP 1 Score: 388.3 bits (996), Expect = 4.2e-104
Identity = 195/206 (94.66%), Postives = 202/206 (98.06%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSRCFVPAMP IFSPRRASPSVGNSQVLFPKSTR PSLCVRAKAAVV+E
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASPSVGNSQVLFPKSTRNPSLCVRAKAAVVNE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSDAVEISFYDLLGIPESGSMAEIKQAYKQ+ALKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYDLLGIPESGSMAEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDP MRALYDRDMLGGLHLAFS+RRRGNNEEMEVKSGWKS WQDQLSGLRRRSMNK
Sbjct: 121 YETLSDPRMRALYDRDMLGGLHLAFSARRRGNNEEMEVKSGWKSLWQDQLSGLRRRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDD+D++
Sbjct: 181 DSGASMSWGARMRRQRDELNDDEDEV 206

BLAST of CmoCh08G009710 vs. NCBI nr
Match: XP_023513423.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.3 bits (991), Expect = 1.6e-103
Identity = 196/206 (95.15%), Postives = 201/206 (97.57%), Query Frame = 0

Query: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60
           MRGYGLALSGTDSR  +PAMP IFSPRRAS SVGNSQVLFPKSTRTPSLCVRAKAAVVDE
Sbjct: 1   MRGYGLALSGTDSRSLLPAMPPIFSPRRASSSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEMEVKSGWKSRWQDQLSGLRRRSMNK 180
           YETLSDPSMRALYDRDMLGGLHLAFS+RRRGNNEEMEVKSGWKSRW+DQLSGLRRRSMNK
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSARRRGNNEEMEVKSGWKSRWEDQLSGLRRRSMNK 180

Query: 181 DSGASMSWGARMRRQRDELNDDDDDM 207
           DSGASMSWGARMRRQRDELNDD+DD+
Sbjct: 181 DSGASMSWGARMRRQRDELNDDEDDV 206

BLAST of CmoCh08G009710 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 174.9 bits (442), Expect = 6.8e-44
Identity = 93/157 (59.24%), Postives = 115/157 (73.25%), Query Frame = 0

Query: 44  TRTPSLCVRAKAAVVDEAVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSP 103
           TRT     R ++ +  +  V  + ++SFYDLLG+ ES ++ EIKQAYKQLA KYHPDVSP
Sbjct: 40  TRTRFSSTRIQSRLTHDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 99

Query: 104 PDRIEEYTRKFIKVQEAYETLSDPSMRALYDRDMLGGLHLAFSSRR--RGNNEEMEVKSG 163
           PDR+EEYT +FI+VQEAYETLSDP  R LYDRD+  G   +FS RR  R + E +E KS 
Sbjct: 100 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSE 159

Query: 164 WKSRWQDQLSGLRRRSMNKDSGASMSWGARMRRQRDE 199
           WK++WQ QLSGLRRRS  KD+  +MSW ARMRRQ+ E
Sbjct: 160 WKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQQQE 195

BLAST of CmoCh08G009710 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 129.4 bits (324), Expect = 3.3e-30
Identity = 63/113 (55.75%), Postives = 82/113 (72.57%), Query Frame = 0

Query: 44  TRTPSLCVRAKAAVVDEAVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSP 103
           TRT     R ++ +  +  V  + ++SFYDLLG+ ES ++ EIKQAYKQLA KYHPDVSP
Sbjct: 40  TRTRFSSTRIQSRLTHDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 99

Query: 104 PDRIEEYTRKFIKVQEAYETLSDPSMRALYDRDMLGGLHLAFSSRRRGNNEEM 157
           PDR+EEYT +FI+VQEAYETLSDP  R LYDRD+  G   +FS RR+   +++
Sbjct: 100 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQV 152

BLAST of CmoCh08G009710 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.9e-12
Identity = 36/82 (43.90%), Postives = 53/82 (64.63%), Query Frame = 0

Query: 70  SFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSM 129
           S+YD+L +P+  S  +IK+AY++LALKYHPD +  +  EE TRKF ++  AYE LSD   
Sbjct: 26  SYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYEVLSDEEK 85

Query: 130 RALYDRDMLGGLHLAFSSRRRG 152
           R +Y++    GL    ++  RG
Sbjct: 86  REIYNKYGEEGLKQFSANGGRG 105

BLAST of CmoCh08G009710 vs. TAIR 10
Match: AT1G59980.1 (ARG1-like 2 )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 72  YDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPSMRA 131
           Y++LGIP + +  EIK AY+++AL+YHPD +P D +      F +V  AYE LSDP  R 
Sbjct: 25  YEVLGIPSNSTDQEIKSAYRRMALRYHPDKNPDDPVA--AEMFKEVTFAYEVLSDPENRR 84

Query: 132 LYD 135
           LYD
Sbjct: 85  LYD 85

BLAST of CmoCh08G009710 vs. TAIR 10
Match: AT5G01390.1 (DNAJ heat shock family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 31/76 (40.79%), Postives = 52/76 (68.42%), Query Frame = 0

Query: 69  ISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPS 128
           + FY +L +  S +  E+K+AY++LA+K+HPD +P ++ +E   KF ++ EAY+ LSDP 
Sbjct: 3   VDFYKVLEVDRSANDDELKKAYRKLAMKWHPDKNPNNK-KEAEAKFKQISEAYDVLSDPQ 62

Query: 129 MRALYDRDMLGGLHLA 145
            RA+Y++    GL+ A
Sbjct: 63  KRAIYEQYGEEGLNQA 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SDN09.6e-4359.24Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2... [more]
Q9WZV35.1e-1249.23Chaperone protein DnaJ OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JC... [more]
A5IIT45.1e-1249.23Chaperone protein DnaJ OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995... [more]
B1LCI25.1e-1249.23Chaperone protein DnaJ OS=Thermotoga sp. (strain RQ2) OX=126740 GN=dnaJ PE=3 SV=... [more]
Q056466.7e-1255.38Chaperone protein DnaJ OS=Erysipelothrix rhusiopathiae OX=1648 GN=dnaJ PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HHM67.3e-110100.00chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KFW22.0e-10494.66chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1H3S23.3e-8681.46chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KVG42.8e-8580.98chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1CE651.9e-7878.28chaperone protein dnaJ 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
XP_022964372.11.5e-109100.00chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata][more]
KAG6593757.12.2e-10596.60Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
KAG7026089.11.5e-10496.12Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_023000441.14.2e-10494.66chaperone protein dnaJ 20, chloroplastic [Cucurbita maxima][more]
XP_023513423.11.6e-10395.15chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G13830.26.8e-4459.24DNAJ-like 20 [more]
AT4G13830.13.3e-3055.75DNAJ-like 20 [more]
AT3G62600.16.9e-1243.90DNAJ heat shock family protein [more]
AT1G59980.11.0e-1049.21ARG1-like 2 [more]
AT5G01390.11.0e-1040.79DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 109..129
score: 41.42
coord: 90..105
score: 51.19
coord: 72..90
score: 43.13
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 69..129
e-value: 8.2E-22
score: 88.5
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 71..134
e-value: 1.9E-21
score: 75.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 70..137
score: 19.840027
IPR001623DnaJ domainCDDcd06257DnaJcoord: 71..126
e-value: 4.75581E-22
score: 82.9785
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 58..163
e-value: 1.3E-22
score: 82.0
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 69..140
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..206
NoneNo IPR availablePANTHERPTHR45090:SF4CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTICcoord: 9..199
NoneNo IPR availablePANTHERPTHR45090CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTICcoord: 9..199
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 114..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G009710.1CmoCh08G009710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061077 chaperone-mediated protein folding
cellular_component GO:0009507 chloroplast