CmoCh08G009060 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G009060
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPeroxisomal membrane protein PEX16
LocationCmo_Chr08: 5896389 .. 5899901 (+)
RNA-Seq ExpressionCmoCh08G009060
SyntenyCmoCh08G009060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCAAATTTGTAGAGAGAGAGGGAGGTTTTTGAGAATGGCGGCGGTAATTGGGGCTGTGGGTCTGATGAAATTTACAGTTTCCGTTTCATAAAATCCCAGTTTGGTTGCTCCTTGTCTCTTCCATTCTTCTCCTAGAATTTCTCCCTTTGTCTGAACCTTTTGGTTTCAACTTTCCCCAAAATTTCACGAACAAGATTCATCTATTTGAATGACCGTACAATTTCCTGTTGGCTATGTGGGAGTCTTTCCCAGTTGAATCTTAGGGTTTTAGTTTGTATCAGGTGCTTGGGCGATTCAAGTGTTTGTTTCCTCTGTTGCTGCAGCGTTGGGATTGTTTTGAAGTCATGGAAGCTTATAAGAAGTGGGTCAGGGATAACAGGAACTATCTTTACTCTTTTGAGTCTCTTGCAAATGTGAGCACTCTCGAATTCTCATCAGAATCTGCTGTTTTATCTGTTTGATTTCATTTGGGAATTGATTTGCTTGGCTTTTCTGACTTTCTCTGTAAGTTATCTTGGAAGCATGCTTATCAACTAGTTTATTTGAATTGCTGTTTAACGATTTTCTTCCCTCAGTTCAATAATCATTGGTTACTTCATTTTCTGGACATACCAATGAAGATTGTATTGCTTACTTATAAACGCATGATCTTCCCCTTAATTAGTCAATGTAGGACTCCCTCCCTACAATTCTCCTCAAATTCTCCCCTCGAACAAATTACATTATAGAGCTTCCCTTGAGACCTATGTAGTGGTCGAACAACTCCCCCTTCATGAGGCTCGACTCCTTTCTCTAGAGCCCTCGAACAAAGTACACTCTTTGTTCGACACTTGAGTCATTTTTTATTACACCTTGGAGGTTAGCAACTTCTTTGTTCGACACTTGAAGATTCGATTAACATGGCTAAGTTAAGGGCAAGACCCTGATACTATATAAGAAATTACGACTCTCTATAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCGTGGTTTTGCTTTTGGTTTCTCCAAAAGGGCTCATACCAATGGAGATAGCATTACTTACTCATAAACCTATGATCTTCCCCTTAATTAGCTAACGTGGGACTCCCTCCCAACTATCCTCATCGTGGATTTGTCATCTTATTTTTGAAAAACAAGATATATAACCAACAATAAGAATCAATTCATCTGAAATTTCTACGTCAATGGATGCCATGTTGTCCATTGTTTCCTAAGGATGCCATGTTTCTCTTATCTTTGATTAGAGAATCTGAGCCTAGACCAGAGACATGACATATCTTTTTCATCTTTGTTTGACATTGAACACCTTAATTTCTTGATAGTTTTTGAGAACTGTTATATTACCAAGCGAAAGTCAACTGTTTGTAGATCTGTTAGTAATAACAACTGGTATAAGTTTGTGGACTTTAACCTCGTATACGGTTCTTAATATTGTACAGGGCTTGACGTGGCTTCTTCCCGAACGGTTTTCTAGTTCAGAAATTGGACCAGAAGCAGGTTTTGTGAAACTTTGGTAACGCTTGACTGCTTCTTAGCTTGGAATATGTGGTGCCCTTGGAGCCCCTTTGATGAAAATGCATGTTTTTATTATTTCTCACTTAAAAAAAAACCATTTTATTCCCTAAAACAGAGAACTAAAACGACTCGATTGTTCTTACGACATTGTTCGACTATGTATACATTTTGATGCCTGTGGTACTGGGTTCTTCTCCTTGCATTATGCTCCGATCTTTGATCTTTGTGGAGGTTTCACCCTTTGATTCTGATACCCAGCAAGGGTTCTTACGTTATTATTAAGCCGTTGTCTTGTGAAATGAACACTGGTTACTGGATCTCATTTGTAAGTTTCAAGCAATTATTCATGAGTTTAGCGATTTCTGCAGTTTCTGCCGCTGTTGGTGTTATCACTGCAGTCAATGAACACATAATTGAAACGGCTCCAACCCAGATGCGTGTAAACGCGACCGTGCCACCTTCATTTCCCTATTCGTTGTGCATATCTGCTCTAAAAAACTTGGAGCCATTGATCGAAGTTGCTGCACAACAATATTATGGCGACGATAAACGCTGGAACTTCATTGCCGTTACGGAGGCTATGAAGTAAGACTCGTTCGAGTATGGGAGTGCCATTTTTGTATCTTAATATACATTTTTATTGCATATTCTTTGCCATTGGATTTCAGGGTACTATTTAGATTAGCTCTGTTCAAGAATAGTGGATACAGAATGATACTCGAGGGAGGCGAAACGACAAATAACGAAACGCATCTTGAAACTTCAACTTCTCAGCACAGAGCTAATGGTGGGCATCATGCATCTGGTTATTTAGGATATGTTAATGGACATAACCAGTCGAGTCTAGAAGGGAGGGCAATGTCTGCTTTAAGTATGTTCGGGGATAATGCTCGGATAAATTCGACACCAGCATGGTCATACCGGGTTCAACACGAGCGTGCAACGTTCAATCCTCCAGGTCGGTATGGATATTATGTATGATTATGATCCCAAGTTGCTAGAGCTTTGTTGGGGATGTCTAATGACAATCCATTCTGTGCCTCTACAGCTACAATACCTGAGAGGTCAACTCTTTCGACTATTTTGTCCGAGCAGGGTCATCTCGGGGCTCTTTTCGTGATAGGAGAAGTGCTTTTTATAACAAGACCGCTAATATACGTTCTTCTAATCCGAAAATACGGGATTCGGTCGTGGACTCCTTGGTTTCTTTCTCTGGCTGTGGACCTGATTGGAACAAGCTTCTTATCATATGCTACCTCAACCCCCAAGAGTAGAAAAGACCAAAGATTTCACCTCTCTGATTCTGAGAAGGACGAGGTCAGGGGTTCTCTCATGCATCATTCATCCCCCATGAATTTGATTGTCATACGTACAGACTAGTTTTCTCGGGTCCGTGCTCGACCCTTTGAACTATCATTCTCGTATTAACATCAACGTGTTCGATTTGGTTCAGCTGAGACGACGAAAGATGCTATGGGCATTTTACATCATGAGAGACCCATTCTTTGAGAGATTCACAAGGTGCATACATACATACTTTAATCTCTCCACTCCAAACTTTAAACCACGACTTGAACTCACTTCGGTTGAGCGTTTTTCTCAGGCGAAAACTCGAAGGAGCCGGGAAAGTGTTGCAACCCGTACCGTTTGTTGGATTCCTCACAGGTTCTGTTTTATCATTATGTTTATGCAGTTCTTCTCATGGTTGTTACAATGTGATTAGTTTTGATGTGCAGCAAAAATGGTGGAGCTTATGGTTGGAGCTCAGACAAGATACGCTTACATGTCTGCTTCATGAGCTGATTGGTTTTTGAAGCTCCAAACTCTTCCTTCCTGCTATCTTTTCTGCTGTTCATAGAAATATTTAGTGTTTTCTTTTAATCAATCTGCTTTGAATAATAATTAGGAATGTATATTAGTATTTTATTTAAAATATGTAATTTAATCACTAATTAAGCCACTAATTGAGTGATTTATGAGTATATTG

mRNA sequence

CAACCAAATTTGTAGAGAGAGAGGGAGGTTTTTGAGAATGGCGGCGGTAATTGGGGCTGTGGGTCTGATGAAATTTACAGTTTCCGTTTCATAAAATCCCAGTTTGGTTGCTCCTTGTCTCTTCCATTCTTCTCCTAGAATTTCTCCCTTTGTCTGAACCTTTTGGTTTCAACTTTCCCCAAAATTTCACGAACAAGATTCATCTATTTGAATGACCGTACAATTTCCTGTTGGCTATGTGGGAGTCTTTCCCAGTTGAATCTTAGGGTTTTAGTTTGTATCAGGTGCTTGGGCGATTCAAGTGTTTGTTTCCTCTGTTGCTGCAGCGTTGGGATTGTTTTGAAGTCATGGAAGCTTATAAGAAGTGGGTCAGGGATAACAGGAACTATCTTTACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCCGAACGGTTTTCTAGTTCAGAAATTGGACCAGAAGCAGTTTCTGCCGCTGTTGGTGTTATCACTGCAGTCAATGAACACATAATTGAAACGGCTCCAACCCAGATGCGTGTAAACGCGACCGTGCCACCTTCATTTCCCTATTCGTTGTGCATATCTGCTCTAAAAAACTTGGAGCCATTGATCGAAGTTGCTGCACAACAATATTATGGCGACGATAAACGCTGGAACTTCATTGCCGTTACGGAGGCTATGAAGGTACTATTTAGATTAGCTCTGTTCAAGAATAGTGGATACAGAATGATACTCGAGGGAGGCGAAACGACAAATAACGAAACGCATCTTGAAACTTCAACTTCTCAGCACAGAGCTAATGGTGGGCATCATGCATCTGGTTATTTAGGATATGTTAATGGACATAACCAGTCGAGTCTAGAAGGGAGGGCAATGTCTGCTTTAAGTATGTTCGGGGATAATGCTCGGATAAATTCGACACCAGCATGGTCATACCGGGTTCAACACGAGCGTGCAACGTTCAATCCTCCAGGTCGGTATGGATATTATGGTCATCTCGGGGCTCTTTTCGTGATAGGAGAAGTGCTTTTTATAACAAGACCGCTAATATACGTTCTTCTAATCCGAAAATACGGGATTCGGTCGTGGACTCCTTGGTTTCTTTCTCTGGCTGTGGACCTGATTGGAACAAGCTTCTTATCATATGCTACCTCAACCCCCAAGAGTAGAAAAGACCAAAGATTTCACCTCTCTGATTCTGAGAAGGACGAGCTGAGACGACGAAAGATGCTATGGGCATTTTACATCATGAGAGACCCATTCTTTGAGAGATTCACAAGGCGAAAACTCGAAGGAGCCGGGAAAGTGTTGCAACCCGTACCGTTTGTTGGATTCCTCACAGGTTCTGTTTTATCATTATGTTTATGCAGTTCTTCTCATGGTTGTTACAATGTGATTAGTTTTGATGTGCAGCAAAAATGGTGGAGCTTATGGTTGGAGCTCAGACAAGATACGCTTACATGTCTGCTTCATGAGCTGATTGGTTTTTGAAGCTCCAAACTCTTCCTTCCTGCTATCTTTTCTGCTGTTCATAGAAATATTTAGTGTTTTCTTTTAATCAATCTGCTTTGAATAATAATTAGGAATGTATATTAGTATTTTATTTAAAATATGTAATTTAATCACTAATTAAGCCACTAATTGAGTGATTTATGAGTATATTG

Coding sequence (CDS)

ATGGAAGCTTATAAGAAGTGGGTCAGGGATAACAGGAACTATCTTTACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCCGAACGGTTTTCTAGTTCAGAAATTGGACCAGAAGCAGTTTCTGCCGCTGTTGGTGTTATCACTGCAGTCAATGAACACATAATTGAAACGGCTCCAACCCAGATGCGTGTAAACGCGACCGTGCCACCTTCATTTCCCTATTCGTTGTGCATATCTGCTCTAAAAAACTTGGAGCCATTGATCGAAGTTGCTGCACAACAATATTATGGCGACGATAAACGCTGGAACTTCATTGCCGTTACGGAGGCTATGAAGGTACTATTTAGATTAGCTCTGTTCAAGAATAGTGGATACAGAATGATACTCGAGGGAGGCGAAACGACAAATAACGAAACGCATCTTGAAACTTCAACTTCTCAGCACAGAGCTAATGGTGGGCATCATGCATCTGGTTATTTAGGATATGTTAATGGACATAACCAGTCGAGTCTAGAAGGGAGGGCAATGTCTGCTTTAAGTATGTTCGGGGATAATGCTCGGATAAATTCGACACCAGCATGGTCATACCGGGTTCAACACGAGCGTGCAACGTTCAATCCTCCAGGTCGGTATGGATATTATGGTCATCTCGGGGCTCTTTTCGTGATAGGAGAAGTGCTTTTTATAACAAGACCGCTAATATACGTTCTTCTAATCCGAAAATACGGGATTCGGTCGTGGACTCCTTGGTTTCTTTCTCTGGCTGTGGACCTGATTGGAACAAGCTTCTTATCATATGCTACCTCAACCCCCAAGAGTAGAAAAGACCAAAGATTTCACCTCTCTGATTCTGAGAAGGACGAGCTGAGACGACGAAAGATGCTATGGGCATTTTACATCATGAGAGACCCATTCTTTGAGAGATTCACAAGGCGAAAACTCGAAGGAGCCGGGAAAGTGTTGCAACCCGTACCGTTTGTTGGATTCCTCACAGGTTCTGTTTTATCATTATGTTTATGCAGTTCTTCTCATGGTTGTTACAATGTGATTAGTTTTGATGTGCAGCAAAAATGGTGGAGCTTATGGTTGGAGCTCAGACAAGATACGCTTACATGTCTGCTTCATGAGCTGATTGGTTTTTGA

Protein sequence

MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIETAPTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRLALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMSALSMFGDNARINSTPAWSYRVQHERATFNPPGRYGYYGHLGALFVIGEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKDELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCYNVISFDVQQKWWSLWLELRQDTLTCLLHELIGF
Homology
BLAST of CmoCh08G009060 vs. ExPASy Swiss-Prot
Match: Q8S8S1 (Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.9e-97
Identity = 197/366 (53.83%), Postives = 250/366 (68.31%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYK+WV  NR Y+ SF S ANGLTWLLPE+FS+SEIGPEAV+A +G+ + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APTQMR--VNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLF 120
           APT      ++   PS  Y L I+ LK+LE ++EVAA+ +YG DK+WN+I +TEAMK + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNSGYRMILEGGETTNNETHLETSTSQHRA-NGGHHASGY-LGYVNGHNQSSLEG 180
           RLALF+NSGY+M+L+GGET N E     S SQ+RA N G +   + LG  N HN  +LEG
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAGNSGRNLGPHGLGNQNHHNPWNLEG 180

Query: 181 RAMSALSMFGDNAR--INSTPAWSYRVQHERATFNPP----------GRYGYYGHLGALF 240
           RAMSALS FG NAR   +STP WS R+QH++A   PP                G  GALF
Sbjct: 181 RAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEPPMIKERRRTMSELLTEKGVNGALF 240

Query: 241 VIGEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHL 300
            IGEVL+ITRPLIYVL IRKYG+RSW PW +SL+VD +G   L  A S     K ++ H 
Sbjct: 241 AIGEVLYITRPLIYVLFIRKYGVRSWIPWAISLSVDTLGMGLL--ANSKWWGEKSKQVHF 300

Query: 301 SDSEKDELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCS 351
           S  EKDELRRRK++WA Y+MRDPFF ++TR+KLE + K L+ +P +GFLT  ++ L   +
Sbjct: 301 SGPEKDELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVELLEGA 360

BLAST of CmoCh08G009060 vs. ExPASy Swiss-Prot
Match: Q2KII7 (Peroxisomal membrane protein PEX16 OS=Bos taurus OX=9913 GN=PEX16 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 4.0e-18
Identity = 90/335 (26.87%), Postives = 151/335 (45.07%), Query Frame = 0

Query: 4   YKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIETAPT 63
           Y+++V  +       E+   GL++LL  RF+ S    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGLSYLLAGRFADSHELSELVYSASNLLVLLNDGIL----- 70

Query: 64  QMRVNATVPPSFPYS---LCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 123
           +  +   +P S         +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+
Sbjct: 71  RKELRKKLPMSLSQQRLLTWLSVLECVEVFMEMGATKVWGEVGRWLVIALIQLAKAVLRM 130

Query: 124 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 183
            L       +  + G  T+          Q ++  G H+SG         QS +  R+  
Sbjct: 131 FLL------IWFKAGLQTSPPIVPLDREIQAQSRDGDHSSG------SQEQSYVGKRSNR 190

Query: 184 ALSMFGDNARINSTPAWSYRVQHERATFNPPGRYGYYGHLGALFVIGEVLFITRPLIYVL 243
            +    +   ++S   W    Q E     P         LG    I E L I RPL+++L
Sbjct: 191 VVRTLQNTPSLHSR-HWGAPQQREELGVAPT-------PLGLQETIAESLHIARPLLHLL 250

Query: 244 LIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKDELRRRKMLWA 303
            +  +G RSWTPW LS  VD+   S LS            R  L+  E+ ELRRR +L  
Sbjct: 251 SLGLWGQRSWTPWLLSGVVDVTSLSLLS-----------DRKGLTRRERLELRRRTILLL 309

Query: 304 FYIMRDPFFERFTRRKLEGAGKVL-QPVPFVGFLT 335
           +Y++R PF++RF+  K+    ++L   VP +G +T
Sbjct: 311 YYLLRSPFYDRFSEAKILFLLQLLADHVPGIGLVT 309

BLAST of CmoCh08G009060 vs. ExPASy Swiss-Prot
Match: Q91XC9 (Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 4.0e-18
Identity = 87/322 (27.02%), Postives = 144/322 (44.72%), Query Frame = 0

Query: 4   YKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIETAPT 63
           Y+++V  +       E+   GL++LL  RFS S    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGLSYLLAGRFSDSHELSELVYSASNLLVLLNDGILR---K 70

Query: 64  QMRVNATVPPSFPYSLC-ISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRLAL 123
           ++R    V  S    L  +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+ L
Sbjct: 71  ELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALIQLAKAVLRMLL 130

Query: 124 FKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMSAL 183
                      G +T+     L+  T     +G H+         G  + S  G+    +
Sbjct: 131 L-----IWFKAGIQTSPPIVPLDRETQAQPLDGDHNP--------GSQEPSYVGKRSHRV 190

Query: 184 SMFGDNARINSTPAWS-------YRVQHERATFNPPGRYGYYGHLGALFVIGEVLFITRP 243
                N+    +  W         + Q +     PP        LG    I E L+I RP
Sbjct: 191 VRTLQNSPSLHSRYWGAPQQREIRQKQQQEELSTPP------TPLGLQETIAESLYIARP 250

Query: 244 LIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKDELRRR 303
           L+++L +  +G RSWTPW LS  VD+   S LS            R +L+  E+ ELRRR
Sbjct: 251 LLHLLSLGLWGQRSWTPWLLSGVVDMTSLSLLS-----------DRKNLTRRERLELRRR 299

Query: 304 KMLWAFYIMRDPFFERFTRRKL 318
            +L  +Y++R PF++RF+  K+
Sbjct: 311 TILLLYYLLRSPFYDRFSEAKI 299

BLAST of CmoCh08G009060 vs. ExPASy Swiss-Prot
Match: Q9Y5Y5 (Peroxisomal membrane protein PEX16 OS=Homo sapiens OX=9606 GN=PEX16 PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 2.6e-17
Identity = 85/333 (25.53%), Postives = 147/333 (44.14%), Query Frame = 0

Query: 4   YKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIETAPT 63
           Y+++V  +       E+   G ++LL  RF+ S    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGFSYLLAGRFADSHELSELVYSASNLLVLLNDGILR---K 70

Query: 64  QMRVNATVPPSFPYSLC-ISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRLAL 123
           ++R    V  S    L  +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+ L
Sbjct: 71  ELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALVQLAKAVLRMLL 130

Query: 124 FKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMSAL 183
                      G +T+     L+  T     +G H    +     G   + +     +  
Sbjct: 131 L-----LWFKAGLQTSPPIVPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTP 190

Query: 184 SMFGDNARINSTPAWSYRVQHERATFNPPGRYGYYGHLGALFVIGEVLFITRPLIYVLLI 243
           S+   +           +  HE  +  P         LG    I E L+I RPL+++L +
Sbjct: 191 SLHSRHWGAPQQREGRQQQHHEELSATPT-------PLGLQETIAEFLYIARPLLHLLSL 250

Query: 244 RKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKDELRRRKMLWAFY 303
             +G RSW PW L+  VD+   S LS            R  L+  E+ ELRRR +L  +Y
Sbjct: 251 GLWGQRSWKPWLLAGVVDVTSLSLLS-----------DRKGLTRRERRELRRRTILLLYY 310

Query: 304 IMRDPFFERFTRRKLEGAGKVL-QPVPFVGFLT 335
           ++R PF++RF+  ++    ++L   VP VG +T
Sbjct: 311 LLRSPFYDRFSEARILFLLQLLADHVPGVGLVT 317

BLAST of CmoCh08G009060 vs. ExPASy Swiss-Prot
Match: B0JYZ2 (Peroxisomal membrane protein PEX16 OS=Xenopus tropicalis OX=8364 GN=pex16 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 8.4e-16
Identity = 83/321 (25.86%), Postives = 139/321 (43.30%), Query Frame = 0

Query: 2   EAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIETA 61
           + YK +V  N       ES    L++L+  RF+ S    E V +A  ++  +N+ I    
Sbjct: 14  QKYKDYVIQNPTGATQLESAVRMLSYLIAGRFADSHELSELVYSASNLLALLNDGI---- 73

Query: 62  PTQMRVNATVPPSFPYS-----LCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKV 121
              +R     PP    S       +  L++LE  IE+ A + +GD  RW  I + + +K 
Sbjct: 74  ---LRKELLAPPPTEGSRRRLLTWLGVLESLEVFIEIGAARAWGDRTRWAAILIIQLLKA 133

Query: 122 LFRLALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEG 181
             R+ L     YR  ++           E   +Q   N    +S ++G  +     SL+ 
Sbjct: 134 CLRIVLL--FWYRAGIQSSPPV-TPLDREGILNQAEDNSNSGSSCFVGRRSSRAVRSLDD 193

Query: 182 RAMSALSMFGDNARINSTPAWSYRVQHERATFNPPGRYGYYGHLGALFVIGEVLFITRPL 241
            A S+   F  + +I+         +          + G    LG L  + E + I RP+
Sbjct: 194 SA-SSHRRFWRSPQIHDG-------KQRNTGETESDKDG--SELGTLGTLAEAIHILRPI 253

Query: 242 IYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKDELRRRK 301
            ++L +  +G +SW PW ++ A+D+   S LS          D R +LS  E+ ELRRR 
Sbjct: 254 THLLSLATWGQKSWKPWMVAAALDITSISLLS----------DVR-NLSHRERAELRRRM 303

Query: 302 MLWAFYIMRDPFFERFTRRKL 318
            L  +Y++R PF+  +T  +L
Sbjct: 314 FLLLYYLLRSPFYNHYTETRL 303

BLAST of CmoCh08G009060 vs. ExPASy TrEMBL
Match: A0A6J1HHK8 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111464386 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.1e-183
Identity = 330/360 (91.67%), Postives = 335/360 (93.06%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS
Sbjct: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300

Query: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCY 351
           ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLT  ++ L + + +   Y
Sbjct: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTAKMVELMVGAQTRYAY 360

BLAST of CmoCh08G009060 vs. ExPASy TrEMBL
Match: A0A6J1HIN9 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111464386 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.7e-179
Identity = 330/389 (84.83%), Postives = 335/389 (86.12%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS
Sbjct: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300

Query: 301 E-----------------------------LRRRKMLWAFYIMRDPFFERFTRRKLEGAG 351
           E                             LRRRKMLWAFYIMRDPFFERFTRRKLEGAG
Sbjct: 301 EVRGSLMHHSSPMNLNYHSRININVFDLVQLRRRKMLWAFYIMRDPFFERFTRRKLEGAG 360

BLAST of CmoCh08G009060 vs. ExPASy TrEMBL
Match: A0A6J1KBV6 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.4e-177
Identity = 320/360 (88.89%), Postives = 329/360 (91.39%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFS+SEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSNSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYG+DKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGNDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGY+MILEGGETTNNETHLETSTSQHRAN GHHASGYLGYV GHNQ SLEGRAMS
Sbjct: 121 ALFKNSGYKMILEGGETTNNETHLETSTSQHRANDGHHASGYLGYVKGHNQWSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                G LGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERPTLLTILSEQGRLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSR+DQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSREDQRFHLSDSEKD 300

Query: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCY 351
           ELRRRKMLWAFYIMRDPFF RFTRRKLEGA KVLQPVPFVGFLT  ++ L + + +   Y
Sbjct: 301 ELRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQTRYTY 360

BLAST of CmoCh08G009060 vs. ExPASy TrEMBL
Match: A0A6J1KE71 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.9e-172
Identity = 320/389 (82.26%), Postives = 329/389 (84.58%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFS+SEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSNSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYG+DKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGNDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGY+MILEGGETTNNETHLETSTSQHRAN GHHASGYLGYV GHNQ SLEGRAMS
Sbjct: 121 ALFKNSGYKMILEGGETTNNETHLETSTSQHRANDGHHASGYLGYVKGHNQWSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                G LGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERPTLLTILSEQGRLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSR+DQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSREDQRFHLSDSEKD 300

Query: 301 E-----------------------------LRRRKMLWAFYIMRDPFFERFTRRKLEGAG 351
           E                             LRRRKMLWAFYIMRDPFF RFTRRKLEGA 
Sbjct: 301 EVRGSLIHHSSPMNLNYHSRINVNEFDLVQLRRRKMLWAFYIMRDPFFGRFTRRKLEGAE 360

BLAST of CmoCh08G009060 vs. ExPASy TrEMBL
Match: A0A1S3CCB9 (Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.5e-156
Identity = 283/365 (77.53%), Postives = 312/365 (85.48%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAY+KWVRDNR+YL+SFESLANGLTWLLPERFS SEIGPEAVSAAVG+ITAVNEHIIET
Sbjct: 1   MEAYRKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQM VNAT+P SFPYSLCI+ALKNLE LIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMHVNATMPSSFPYSLCIAALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRAN-----GGHHASGYLGYVNGHNQSSLE 180
           ALFKNSGY+++LEGGETTNNE HLETSTSQH+AN     GGHHASG+ GY+NGHN  +LE
Sbjct: 121 ALFKNSGYKILLEGGETTNNERHLETSTSQHKANAFTKHGGHHASGFSGYLNGHNPWNLE 180

Query: 181 GRAMSALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFV 240
           GRA+SALS FG+NA++ S PAWS+RVQH+ A  NPP                GH GALF+
Sbjct: 181 GRALSALSRFGENAQMISPPAWSHRVQHQHAILNPPATIPERPTLSTILSEQGHRGALFI 240

Query: 241 IGEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLS 300
            GEVLFITRPLIYVLLIRKYG RSWTPWFLSLAVDL+GTSFLSYATS   SRKDQR  LS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASASRKDQRLRLS 300

Query: 301 DSEKDELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSS 351
           DSEKDELRRRKMLWAFY+MRDPFFER+TRRKLEGA KVL+PVPFVGFLT  ++ L + + 
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIVGAQ 360

BLAST of CmoCh08G009060 vs. NCBI nr
Match: XP_022964352.1 (peroxisome biogenesis protein 16 isoform X2 [Cucurbita moschata])

HSP 1 Score: 652.5 bits (1682), Expect = 2.2e-183
Identity = 330/360 (91.67%), Postives = 335/360 (93.06%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS
Sbjct: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300

Query: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCY 351
           ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLT  ++ L + + +   Y
Sbjct: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTAKMVELMVGAQTRYAY 360

BLAST of CmoCh08G009060 vs. NCBI nr
Match: KAG6593694.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 641.3 bits (1653), Expect = 5.1e-180
Identity = 324/360 (90.00%), Postives = 332/360 (92.22%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQ+RVN+TVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQLRVNSTVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQ SLEGRAMS
Sbjct: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQWSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFV+GEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVMGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300

Query: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCY 351
           ELRRRKM WAFYIMRDPFFERFTRRKLEGA KVLQPVPFVGFLT  ++ L + + +   Y
Sbjct: 301 ELRRRKMQWAFYIMRDPFFERFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQTRYTY 360

BLAST of CmoCh08G009060 vs. NCBI nr
Match: XP_022964351.1 (peroxisome biogenesis protein 16 isoform X1 [Cucurbita moschata])

HSP 1 Score: 637.1 bits (1642), Expect = 9.7e-179
Identity = 330/389 (84.83%), Postives = 335/389 (86.12%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS
Sbjct: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300

Query: 301 E-----------------------------LRRRKMLWAFYIMRDPFFERFTRRKLEGAG 351
           E                             LRRRKMLWAFYIMRDPFFERFTRRKLEGAG
Sbjct: 301 EVRGSLMHHSSPMNLNYHSRININVFDLVQLRRRKMLWAFYIMRDPFFERFTRRKLEGAG 360

BLAST of CmoCh08G009060 vs. NCBI nr
Match: XP_022999827.1 (peroxisome biogenesis protein 16 isoform X2 [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 9.1e-177
Identity = 320/360 (88.89%), Postives = 329/360 (91.39%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFS+SEIGPEAVSAAVGVITAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSNSEIGPEAVSAAVGVITAVNEHIIET 60

Query: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120
           APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYG+DKRWNFIAVTEAMKVLFRL
Sbjct: 61  APTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGNDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEGRAMS 180
           ALFKNSGY+MILEGGETTNNETHLETSTSQHRAN GHHASGYLGYV GHNQ SLEGRAMS
Sbjct: 121 ALFKNSGYKMILEGGETTNNETHLETSTSQHRANDGHHASGYLGYVKGHNQWSLEGRAMS 180

Query: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVIGEVL 240
           ALSMFGDNARINSTPAWSYRVQHERATFNPP                G LGALFVIGEVL
Sbjct: 181 ALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERPTLLTILSEQGRLGALFVIGEVL 240

Query: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSDSEKD 300
           FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSR+DQRFHLSDSEKD
Sbjct: 241 FITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSREDQRFHLSDSEKD 300

Query: 301 ELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCSSSHGCY 351
           ELRRRKMLWAFYIMRDPFF RFTRRKLEGA KVLQPVPFVGFLT  ++ L + + +   Y
Sbjct: 301 ELRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQTRYTY 360

BLAST of CmoCh08G009060 vs. NCBI nr
Match: KAG7026034.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.2 bits (1598), Expect = 1.2e-173
Identity = 324/393 (82.44%), Postives = 332/393 (84.48%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPE----AVSAAVGVITAVNEH 60
           MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPE    AVSAAVGVITAVNEH
Sbjct: 11  MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAAISAVSAAVGVITAVNEH 70

Query: 61  IIETAPTQMRVNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKV 120
           IIETAPTQ+RVN+TVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKV
Sbjct: 71  IIETAPTQLRVNSTVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKV 130

Query: 121 LFRLALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQSSLEG 180
           LFRLALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQ SLEG
Sbjct: 131 LFRLALFKNSGYRMILEGGETTNNETHLETSTSQHRANGGHHASGYLGYVNGHNQWSLEG 190

Query: 181 RAMSALSMFGDNARINSTPAWSYRVQHERATFNPPGR----------YGYYGHLGALFVI 240
           RAMSALSMFGDNARINSTPAWSYRVQHERATFNPP                GHLGALFV+
Sbjct: 191 RAMSALSMFGDNARINSTPAWSYRVQHERATFNPPATIPERSTLSTILSEQGHLGALFVM 250

Query: 241 GEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSD 300
           GEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSD
Sbjct: 251 GEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHLSD 310

Query: 301 SEKDE-----------------------------LRRRKMLWAFYIMRDPFFERFTRRKL 351
           SEKDE                             LRRRKM WAFYIMRDPFFERFTRRKL
Sbjct: 311 SEKDEVRGSLIHHSSPMNLNYHSRINVNVFDLVQLRRRKMQWAFYIMRDPFFERFTRRKL 370

BLAST of CmoCh08G009060 vs. TAIR 10
Match: AT2G45690.1 (shrunken seed protein (SSE1) )

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-98
Identity = 197/366 (53.83%), Postives = 250/366 (68.31%), Query Frame = 0

Query: 1   MEAYKKWVRDNRNYLYSFESLANGLTWLLPERFSSSEIGPEAVSAAVGVITAVNEHIIET 60
           MEAYK+WV  NR Y+ SF S ANGLTWLLPE+FS+SEIGPEAV+A +G+ + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APTQMR--VNATVPPSFPYSLCISALKNLEPLIEVAAQQYYGDDKRWNFIAVTEAMKVLF 120
           APT      ++   PS  Y L I+ LK+LE ++EVAA+ +YG DK+WN+I +TEAMK + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNSGYRMILEGGETTNNETHLETSTSQHRA-NGGHHASGY-LGYVNGHNQSSLEG 180
           RLALF+NSGY+M+L+GGET N E     S SQ+RA N G +   + LG  N HN  +LEG
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAGNSGRNLGPHGLGNQNHHNPWNLEG 180

Query: 181 RAMSALSMFGDNAR--INSTPAWSYRVQHERATFNPP----------GRYGYYGHLGALF 240
           RAMSALS FG NAR   +STP WS R+QH++A   PP                G  GALF
Sbjct: 181 RAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEPPMIKERRRTMSELLTEKGVNGALF 240

Query: 241 VIGEVLFITRPLIYVLLIRKYGIRSWTPWFLSLAVDLIGTSFLSYATSTPKSRKDQRFHL 300
            IGEVL+ITRPLIYVL IRKYG+RSW PW +SL+VD +G   L  A S     K ++ H 
Sbjct: 241 AIGEVLYITRPLIYVLFIRKYGVRSWIPWAISLSVDTLGMGLL--ANSKWWGEKSKQVHF 300

Query: 301 SDSEKDELRRRKMLWAFYIMRDPFFERFTRRKLEGAGKVLQPVPFVGFLTGSVLSLCLCS 351
           S  EKDELRRRK++WA Y+MRDPFF ++TR+KLE + K L+ +P +GFLT  ++ L   +
Sbjct: 301 SGPEKDELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVELLEGA 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S8S11.9e-9753.83Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 S... [more]
Q2KII74.0e-1826.87Peroxisomal membrane protein PEX16 OS=Bos taurus OX=9913 GN=PEX16 PE=2 SV=2[more]
Q91XC94.0e-1827.02Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2[more]
Q9Y5Y52.6e-1725.53Peroxisomal membrane protein PEX16 OS=Homo sapiens OX=9606 GN=PEX16 PE=1 SV=2[more]
B0JYZ28.4e-1625.86Peroxisomal membrane protein PEX16 OS=Xenopus tropicalis OX=8364 GN=pex16 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1HHK81.1e-18391.67Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111464386... [more]
A0A6J1HIN94.7e-17984.83Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111464386... [more]
A0A6J1KBV64.4e-17788.89Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 P... [more]
A0A6J1KE711.9e-17282.26Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 P... [more]
A0A1S3CCB92.5e-15677.53Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_022964352.12.2e-18391.67peroxisome biogenesis protein 16 isoform X2 [Cucurbita moschata][more]
KAG6593694.15.1e-18090.00Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022964351.19.7e-17984.83peroxisome biogenesis protein 16 isoform X1 [Cucurbita moschata][more]
XP_022999827.19.1e-17788.89peroxisome biogenesis protein 16 isoform X2 [Cucurbita maxima][more]
KAG7026034.11.2e-17382.44Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
AT2G45690.11.4e-9853.83shrunken seed protein (SSE1) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013919Peroxisome membrane protein, Pex16PFAMPF08610Pex16coord: 2..338
e-value: 8.9E-71
score: 239.0
IPR013919Peroxisome membrane protein, Pex16PANTHERPTHR13299UNCHARACTERIZEDcoord: 1..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G009060.1CmoCh08G009060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007031 peroxisome organization
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005778 peroxisomal membrane