CmoCh08G006540 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G006540
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Regulator of Vps4 activity in the MVB pathway protein, putative
Location	Cmo_Chr08: 4251602 .. 4254054 (-)
RNA-Seq Expression	CmoCh08G006540
Synteny	CmoCh08G006540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCATCTTCTTACACAGCTGGGTTTTCCGGTATCTTTTTGGCGGGTTTTCGATTCTTCTTCTTCTTCTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTCTTCTTTTTTAATAAACCCTTTTTTTTTGGAGTTCATTTTTGTCGGTGAGCTTCTCTGTTTCTCTTGCACTGTCGTGTCTGTGTTGTATGCTTCATGTTCATCTTCCATTTGGCTCATTATTACTCGTTCCTCCTTTTGATGTTTCGTAAACATTCTTCAATGGCGGATGTGGGATTATTCCATTTTCTTCATCCCTTCTTTTAATTGTTTTTGGAATCTTGGTTGTGGGTTTCTTGGGTTTTTCATTCTCCATTGCCTGATTCTTCGGTGTTCTTCCTCGTTTCAGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGGTATTGATTCTGATTCTGATTCTGATTCTGATTCTGATTCCGATTCCGATTCCGATTCCGATTCCGATTTTTATGGGTTTCTTTTGATTGATTCTTTCTTTTATTGTGATGTAGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCTTCTTCGTGTCTTGGATTAGATTCTTAATCTCTCCGAAACCCATAATTTTAATATCTGTCTTCTGTTGTGATTGTTCTTAAATTCTTTGTGTTTGGATTGACTGTAAGAGAATTTCTGGTTCATCTTCCCTGATTTTGGTCGTGCTTGTTGTGCTAATGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGGTAGTAGATATCATGATTTTTGGAACATGTCTGTGTTCTTAGGCTTCAATTTGTTCAAATCAGTTTAAATGTGGTTTCCATGTTTTTGTTTCTCTTCTAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGGTTGTTCTTGGTAGCTTAAGAACTCGGTTGTTTCGTCAGAGGGTCGTCCTAAAACTTGGTTTAATTCTTGTTTTTCCTTTTAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAAGTGAGTTTGTGTGCAATTCTGTTTTTGTGTGTTGATGAACTCTCTTCATTCTATTGATGGTTTTATGGTCGATCTTGTTGCAGCCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCGCGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAAAAGGTGTCGGTATCAGACGCTCGAGAAAATTATCGATGGAACATGGTTGACATGACATTAAGAAGGAGACACCATCCTAAAAGAAGATTCTTTGCTTCGAAACTAAACCAATAGGACACTTGCAATTCTCTAATCATTTCTTACAACCCACCTGTACTTTATAACTTTAAGAAAATGC

mRNA sequence

TCTTCATCTTCTTACACAGCTGGGTTTTCCGGTATCTTTTTGGCGGGTTTTCGATTCTTCTTCTTCTTCTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTCTTCTTTTTTAATAAACCCTTTTTTTTTGGAGTTCATTTTTGTCGGTGAGCTTCTCTGTTTCTCTTGCACTGTCGTGTCTGTGTTGTATGCTTCATGTTCATCTTCCATTTGGCTCATTATTACTCGTTCCTCCTTTTGATGTTTCGTAAACATTCTTCAATGGCGGATGTGGGATTATTCCATTTTCTTCATCCCTTCTTTTAATTGTTTTTGGAATCTTGGTTGTGGGTTTCTTGGGTTTTTCATTCTCCATTGCCTGATTCTTCGGTGTTCTTCCTCGTTTCAGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCGCGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAAAAGGTGTCGGTATCAGACGCTCGAGAAAATTATCGATGGAACATGGTTGACATGACATTAAGAAGGAGACACCATCCTAAAAGAAGATTCTTTGCTTCGAAACTAAACCAATAGGACACTTGCAATTCTCTAATCATTTCTTACAACCCACCTGTACTTTATAACTTTAAGAAAATGC

Coding sequence (CDS)

ATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCGCGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAA

Protein sequence

MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAALKEDK

Homology

[Close]

BLAST of CmoCh08G006540 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.3e-13
Identity = 47/181 (25.97%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 13  KCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCY 72
           K K  LKL  SR+  ++ KK  +++  K ++ ELL+ + + +A  R E  + +  ++ C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  ELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG- 132
           ++I+  C  +  +++++   +E P E  E++ +L+Y++ R   +PEL +++N    KYG 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 -------CSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPP 186
                  C      + +++ K         I  Q L EIA++  +DW         Y PP
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD-----YPPP 186

[Close]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL
Match: A0A6J1HLZ1 (uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC111464187 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.5e-231
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 426
           LKEDK
Sbjct: 421 LKEDK 425

[Close]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL
Match: A0A6J1KJN9 (uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.7e-214
Identity = 402/426 (94.37%), Postives = 409/426 (96.01%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD-EEEEEKMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD EEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEP   TELENK HTRTNSFVHP LPDYD+LAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEP---TELENKRHTRTNSFVHPNLPDYDELAARFA 420

Query: 421 ALKEDK 426
           ALKEDK
Sbjct: 421 ALKEDK 423

[Close]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 2.4e-139
Identity = 305/468 (65.17%), Postives = 342/468 (73.08%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD IRKKKN VLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST D STDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKE 360
           E N  +S PT E   KPKP+SVRRRNVK QP  +I I +V +S  D    ISSSRNKGKE
Sbjct: 301 ESNNPKS-PTEE---KPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGEGDD-EEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VR 420
            M GE     ++EE++LDGLLM YSKKK + ES                          R
Sbjct: 361 TMIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTR 420

Query: 421 AVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 424
           AVSL  D  E T       K HTRTNSFVHPKLP+YD+LAAR AALKE
Sbjct: 421 AVSLPTDQNEST-------KKHTRTNSFVHPKLPEYDQLAARIAALKE 454

[Close]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 3.4e-138
Identity = 295/472 (62.50%), Postives = 329/472 (69.70%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMF KPK++TKCKSC+KLLK RLD IRKKKN VLK+LK+DI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQLSVL KQSECPDEC EAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR+ FT KYG SFE FT+ ELIEK  A   +KE+KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YIPPPNEPD--------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETST 240
           Y PP N PD               +PV ERK S + K+D+SDD+S+FD RSE N TETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 VDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERN 300
            DSSTDQDV+KG  EDEVED+ PFYLR I PPYLK+KP+ KE   E              
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE-------------- 300

Query: 301 KQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKEAMK 360
               +P  EE  KPKP+SVRRRN KL+PT +I IGNV+NS ND     SSSRNKGKE M 
Sbjct: 301 ----KPAVEE--KPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMM 360

Query: 361 GEGDDEEEEEKMLDGLLMHYSKKKPSHESNEV---------------------------- 420
           GE     EEE+MLDGLLMHYSKKK + ESN                              
Sbjct: 361 GEEKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLS 420

Query: 421 ---RAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 424
              R+VSL  D+ EP     +  K HTR NSFVHPKLPDYD+LAARFAALKE
Sbjct: 421 VPGRSVSLPRDTNEPI----VPMKRHTRNNSFVHPKLPDYDQLAARFAALKE 448

[Close]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 4.8e-132
Identity = 292/467 (62.53%), Postives = 329/467 (70.45%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD  RKKKN VLKYLK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC E+VA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSR T Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+E+K +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE 300
           ST D STDQDV KG S DEV DQ PF  R ++PPYLKTKP   E   E       +ENDE
Sbjct: 241 STGD-STDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDE 300

Query: 301 RNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKEA 360
            N     P S  + KPKP+SVRRR VK QP  +I I +V +S  D    ISS RNKGKE 
Sbjct: 301 SN-----PKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKET 360

Query: 361 MKGEGDD-EEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VRA 420
           M GE     ++EE++LDGLLM YSKKK + ES                          RA
Sbjct: 361 MIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRA 420

Query: 421 VSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 424
           VS   D  EP        K HTRTNSFVHPKLP+YD+LAAR AALKE
Sbjct: 421 VSFPPDQNEPM-------KKHTRTNSFVHPKLPEYDQLAARIAALKE 453

[Close]

BLAST of CmoCh08G006540 vs. NCBI nr
Match: XP_022964044.1 (uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 811.6 bits (2095), Expect = 3.2e-231
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 426
           LKEDK
Sbjct: 421 LKEDK 425

[Close]

BLAST of CmoCh08G006540 vs. NCBI nr
Match: KAG6593450.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 800.0 bits (2065), Expect = 9.6e-228
Identity = 421/425 (99.06%), Postives = 422/425 (99.29%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEP   TELENKHHTRTNSFVHPKLPDYDKLAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEP---TELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 426
           LKEDK
Sbjct: 421 LKEDK 422

[Close]

BLAST of CmoCh08G006540 vs. NCBI nr
Match: XP_023514654.1 (uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 779.2 bits (2011), Expect = 1.8e-221
Identity = 411/425 (96.71%), Postives = 416/425 (97.88%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVH RKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDERNKQQ EPT EE  KP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEE--KP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPT+EIKI NV NSGND+SSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHH RTNSFVHPKLPDYD+LAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAA 420

Query: 421 LKEDK 426
           LKEDK
Sbjct: 421 LKEDK 423

[Close]

BLAST of CmoCh08G006540 vs. NCBI nr
Match: XP_023000359.1 (uncharacterized protein LOC111494616 [Cucurbita maxima])

HSP 1 Score: 755.0 bits (1948), Expect = 3.5e-214
Identity = 402/426 (94.37%), Postives = 409/426 (96.01%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD-EEEEEKMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD EEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEP   TELENK HTRTNSFVHP LPDYD+LAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEP---TELENKRHTRTNSFVHPNLPDYDELAARFA 420

Query: 421 ALKEDK 426
           ALKEDK
Sbjct: 421 ALKEDK 423

[Close]

BLAST of CmoCh08G006540 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 505.4 bits (1300), Expect = 4.9e-139
Identity = 305/468 (65.17%), Postives = 342/468 (73.08%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD IRKKKN VLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST D STDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKE 360
           E N  +S PT E   KPKP+SVRRRNVK QP  +I I +V +S  D    ISSSRNKGKE
Sbjct: 301 ESNNPKS-PTEE---KPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGEGDD-EEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VR 420
            M GE     ++EE++LDGLLM YSKKK + ES                          R
Sbjct: 361 TMIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTR 420

Query: 421 AVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 424
           AVSL  D  E T       K HTRTNSFVHPKLP+YD+LAAR AALKE
Sbjct: 421 AVSLPTDQNEST-------KKHTRTNSFVHPKLPEYDQLAARIAALKE 454

[Close]

BLAST of CmoCh08G006540 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.2e-60
Identity = 143/357 (40.06%), Postives = 214/357 (59.94%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD   KPK+YTKCKS +K+ K+R+D +++KKN+V KYLK+DIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           +LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YIPP--------PNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDSS 240
           Y PP          EP     +   + ++ KR S       D   + N +   TS  DS 
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDSM 240

Query: 241 TDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDERNKQQ 300
           +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +      
Sbjct: 241 STSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE------ 300

Query: 301 SEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVE---NSGNDISSSRNKGKEAMKG 344
             PT     KPKP+SVRRR     P  E +   ++    S N + +S + GK + +G
Sbjct: 301 -SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 346

[Close]

BLAST of CmoCh08G006540 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 152.5 bits (384), Expect = 7.5e-37
Identity = 105/298 (35.23%), Postives = 164/298 (55.03%), Query Frame = 0


Query: 60  EGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 179
           R+LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYIPP--------PNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDS 239
           LY PP          EP     +   + ++ KR S       D   + N +   TS  DS
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDS 225

Query: 240 STDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDERNKQ 299
            +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +     
Sbjct: 226 MSTSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE----- 285

Query: 300 QSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVE---NSGNDISSSRNKGKEAMKG 344
              PT     KPKP+SVRRR     P  E +   ++    S N + +S + GK + +G
Sbjct: 286 --SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 332

[Close]

BLAST of CmoCh08G006540 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-32
Identity = 82/223 (36.77%), Postives = 130/223 (58.30%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD FL   +  K K  +KL K+R+D +R+K+N  +K+LK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G L E   L   + +++ C  +  QLS + K  ECP++C EA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR  F  KY  S   F + EL+E   +   S E K++L++++A E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTE 224
                   + + V E  KS+  K    D       R E   +E
Sbjct: 181 V-----RQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSE 218

HSP 2 Score: 32.0 bits (71), Expect = 1.5e+00
Identity = 44/156 (28.21%), Postives = 75/156 (48.08%), Query Frame = 0


Query: 236 RKGGSEDEVEDQTPFYLRVIRPPYL-----KTKPTNKETMEEARKATDQVENDERNKQQS 295
           RK    +    ++PFY + + PPY+     K +    E ++  +   D  E +  +  ++
Sbjct: 502 RKVEERETERMKSPFY-KSLPPPYVKKSIAKARHEKAEALDNPKARFDGEEGNHPDNGKN 561

Query: 296 EPTSEEKPKPKPKSVRR-RNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKG-----E 355
              +E +       V    N  L+     +   VE+ G+D  SSR + + + KG     +
Sbjct: 562 VYGAERRNGAGHHEVNDIDNASLKRQTNRRKHIVESGGDDHISSRRR-ENSRKGLQVLID 621

Query: 356 GDDEEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLS 381
            D+++ EEKM+D LLMHYSKK  S+E + V+  S S
Sbjct: 622 EDEKDSEEKMMDKLLMHYSKKPSSYEKDNVQEESKS 655

[Close]

BLAST of CmoCh08G006540 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 136.7 bits (343), Expect = 4.3e-32
Identity = 97/304 (31.91%), Postives = 164/304 (53.95%), Query Frame = 0


Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+Y K KS    +K R+D +R+K+  +++  K+DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  IS  LS+++K+ ECP+EC EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSR-ATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 180
           +LR  FT ++G       + EL+EK+      S+E+K+Q ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYIPPPNEPDVVPVHERKKSSVG----------KRDSSDDESM------FDIRSEGNTTE 240
           L      E   + + ++ ++               + SDDES+       D  S+G+ + 
Sbjct: 181 LL----RETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLSKGSLSS 240

Query: 241 TSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVEND 288
           +S+  SS+ + V    S  + + +      +I  P  K    N E  +E ++   ++ + 
Sbjct: 241 SSSSSSSSLRRV----SVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEKRKIADQ 296

[Close]

BLAST of CmoCh08G006540 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 110.9 bits (276), Expect = 2.5e-24
Identity = 116/441 (26.30%), Postives = 207/441 (46.94%), Query Frame = 0


Query: 12  TKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRC 71
           +KCK+  K+  +R+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG 131
            E+I+ FC  I ++L+++ KQ +CP +  E +ASLI+AA R +++PEL +LR+ F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 CSF-------EPFTSIE--LIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYI 191
             F        P   +   LI+K        E KL++++EIA+E  +DW++   EQ+L +
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-L 208

Query: 192 PPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSED 251
            P  E    P      SS+    ++ +E +   ++   +T + ++++          +  
Sbjct: 209 KPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 EVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVEN-----------DERNKQQSE 311
           E+  Q     +V      +   +NKE    +  +T Q ++             R  + SE
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSE 328

Query: 312 PTS-EEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEE 371
            +S   KP  + + + RR+    P +     N  +   + +++  + KE M+        
Sbjct: 329 TSSYYAKPGAENRGMGRRHSYNNPGI-----NESDYEEEYTNTEAEAKETMRRRHSYNPR 388

Query: 372 E------EKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFV 426
                   ++      +Y ++    E ++ R  SL  +   P      E++  +  +  V
Sbjct: 389 SVPPPATSEIKFDESDYYEEETEPDEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQ-V 448

The following BLAST results are available for this feature:

BLAST of CmoCh08G006540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54I39	4.3e-13	25.97	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]

BLAST of CmoCh08G006540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HLZ1	1.5e-231	100.00	uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC1114641...	[more]
A0A6J1KJN9	1.7e-214	94.37	uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616...	[more]
A0A1S3CGT5	2.4e-139	65.17	uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=...	[more]
A0A6J1CWS8	3.4e-138	62.50	vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 ...	[more]
A0A0A0K926	4.8e-132	62.53	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1	[more]

BLAST of CmoCh08G006540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022964044.1	3.2e-231	100.00	uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-lik...	[more]
KAG6593450.1	9.6e-228	99.06	IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023514654.1	1.8e-221	96.71	uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo]	[more]
XP_023000359.1	3.5e-214	94.37	uncharacterized protein LOC111494616 [Cucurbita maxima]	[more]
XP_008462153.1	4.9e-139	65.17	PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo]	[more]

BLAST of CmoCh08G006540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G79910.1	2.2e-60	40.06	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G79910.2	7.5e-37	35.23	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G32350.1	1.1e-32	36.77	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G52315.1	4.3e-32	31.91	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G35730.1	2.5e-24	26.30	Regulator of Vps4 activity in the MVB pathway protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 143..163
None	No IPR available	COILS	Coil	Coil	coord: 266..286
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 265..302
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 234..249
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..216
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 364..406
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 217..233
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 182..352
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 319..334
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 373..387
None	No IPR available	PANTHER	PTHR12161:SF60	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 9..423
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 6..177 e-value: 1.6E-45 score: 156.9
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 15..169 e-value: 1.6E-39 score: 135.5
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 9..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G006540.1	CmoCh08G006540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport