CmoCh08G006130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G006130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAcyl-[acyl-carrier-protein] hydrolase
LocationCmo_Chr08: 3737739 .. 3740348 (+)
RNA-Seq ExpressionCmoCh08G006130
SyntenyCmoCh08G006130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTTTGTGGAGGACCATTACTTTACTGGAACTCAAAATCTGTTCTTTCTTCTCTAACTGCAACTCCCAAGGATATTGTAGCTATGATTTTTTCGATTCAAGTATAGAATTTCGCTCGAGGCTAATACTTGGAAGTCAATCTTATCAATAATATCATATTAACGAGTGATGGTTGGATCCAAATAAATAAAAAATTTTGAGTTCATACATCTTATTAAATATTTGAAAATAAATAAATGGGTGTTATACAAACTAACATTTACTTTTGTTAAATAAAGTTTTTGAATAAATGTTTCAAATAACTTAATCAACTCAACTCAAAAAATTTATGGTTGGGCTGAGTTGGATTCATTATTTATTGACTGGACTTAGAAATTTGCATTAAGTGGATCATAAATTTCTCTATAATAGAACGTCTTAGAGGGGAAACTACGAGGACATCGAAAAGATGTTGATATTGACAATGGATATAGTTTCCTCCAATGAGATTGTGATTGCCTTTTAAACATGGGTTGGCATTGTTGTTTTATATAAAAAGTATATATATGTAATGATTCAAACTTTTTTTCGACTTAGATCACCACGAAATCTATTAAGAAACAATGAAAACCGTTAGTAATAACTTTGAACTTAAAAAACGTTTCTTAAAAATTTAAAAGTTTCATTAATAGTTAGTGTTAAAAGTAACATTAATATACAAAATCCCTCGGAACTAAATATTAAAAAAAATAGAATTTAAAAGAAGGTAAAAAAATTATGAATTGATTATAAAAACTAGAACGTCTTCCAACATCAGTTTAAAATTAATGAAAAACAACATTAATGAAACAGCTTTAATGTAATATTAAAAAAAAAACTGAAATTCAAAAAAAAAAAATCCTATAAATGGTTCTCTTGTGTCATCTAAGGTCCAACTCCATCAAATCTTATGGTAAGTTTGATATCATGGCACTCATATCTTCATCAAATGGCTCCCATTTTGGTATGGAATTAGTATCAATTTCAATGAGGGAACCAACATTTGGACATGCCACCACACCCAGTTTGCACCTCAAAGGCCGACCCAAAAGTGCCTCTCTTACCCTCAAATCTTCTCACATTTCTCAACCCACTGCAGGTAAGTTGATGATGATATTAAAGATCTTGCCTTTTCCAACCCTTCTTTTGTGGGTCACTCTCCTTTTTTGGTAGGTGGGTTCGAGAGTATGGATGATTACATGTCGAGAAAACCCGAGATAACTCGTGAGAATTTAATGGTGGAAGAGTTTTTGAACGATGGTTTGGTTTACCAATGTCATTTTTTGGTGAGATCATACGAGTCGGGACCTGATAATAAGATGACAATGTTTTTCGTCATCAACCAGTTACAGGTTCTAAGTTTATTTGTTAAAAAAAAACATGTCTATAAATGAAATGTGAATCTCTTTTGATACGCGGGTTTTGATTAGGAGTCGAAACTAAATCACTTCATAAGTTGTGGATTGGTCGAAAGGGGATTGGGCAAGACAGGATTTGGCACAACACATGAAATGACTAAAAGGGACCTCATATGGGTCGTGCATAAATTGCAAGTGGTGGTCGACCAATTTCCTTCTTGGTAAATATAACATATTTTTTTACATTCCCGTGTTGCTAAGATGTTCGAGTAACGTGGTTTTAAAACACGAATTTGAAGGAACGACGTCGTTGAACTTCGTACGTGGATATGTAAACATGGGAAGAATAGCTATCGTCGAAACTGGATCGCTCGTGACAAGAAAAGTGGAAAAGTTTTGATCCGTGCAATTTGGTTTGTTCTTGATCCCGATACAAACGCCGTTGAAATTGAAACAACTCTATGTTTTGATATTTCGTTTGCGTTGCAGTTTCTATGTTTTGATGAACAGAAGAACAAGAAAATTATCCAAATTTTGTGAAGAATTAAAGGAGGAAATGAATCCATTCTTCTTAGACAGCCATCCAATCCTTGAGGAAGATAACATCGTCTCACAAAAACTACATTTTCATCAACATTATTGCACCCATGCATCTTTAGTTGTAAGTTATAATTTTAATAACTCTTTTTTTATTTTTTTTTATTTAACTTAATTTTACTCGTGACTCTTCAAATATTGGTAGACGAGACTGAGCGATTTGGATATGAATCAGCACATTAACAATGCCAGTTATATCAACTTCATTCTCGAGGTATGTTTACCTACCTTTTTCTTGTTATAAATAATTTCTTTTACTTATTTAAATCTTTAATTTGTTTTTCAAATTCAATTAAAATGTTTGAAAAAAACATAGAACTAACTGTGCGACTATGAAAAACACTACGAAAACAATTTTCTAACGAGAATGAGAAAAAAAATGTCAATATTTTGGTTTTAGGGTACTCCTCGATCGGTGTTGGAGAAATACCAGCTCTCGGGTATAACAATCAAGTACTTAAAAGAATGTAGTGTGGACCAACAATTGCAGTCGCTTTCTGTGCCCTTAGAAAACGACGTTGTGAATGGTACTCCTCTCATCAAAGATAAAGACATCAAACTCCACCACTCAATTCTCTCTGATGGGATTGAACTTGTTCGAGCAAAAACCGTTTGGACACCAAAATTCAATTAA

mRNA sequence

ATGAGGTCCAACTCCATCAAATCTTATGGTAAGTTTGATATCATGGCACTCATATCTTCATCAAATGGCTCCCATTTTGGTATGGAATTAGTATCAATTTCAATGAGGGAACCAACATTTGGACATGCCACCACACCCAGTTTGCACCTCAAAGGCCGACCCAAAAGTGCCTCTCTTACCCTCAAATCTTCTCACATTTCTCAACCCACTGCAGGTGGGTTCGAGAGTATGGATGATTACATGTCGAGAAAACCCGAGATAACTCGTGAGAATTTAATGGTGGAAGAGTTTTTGAACGATGGTTTGGTTTACCAATGTCATTTTTTGGTGAGATCATACGAGTCGGGACCTGATAATAAGATGACAATGTTTTTCGTCATCAACCAGTTACAGGAGTCGAAACTAAATCACTTCATAAGTTGTGGATTGGTCGAAAGGGGATTGGGCAAGACAGGATTTGGCACAACACATGAAATGACTAAAAGGGACCTCATATGGGTCGTGCATAAATTGCAAGTGGTGGTCGACCAATTTCCTTCTTGGAACGACGTCGTTGAACTTCGTACGTGGATATGTAAACATGGGAAGAATAGCTATCGTCGAAACTGGATCGCTCGTGACAAGAAAAGTGGAAAAGTTTTGATCCGTGCAATTTGTTTCTATGTTTTGATGAACAGAAGAACAAGAAAATTATCCAAATTTTGTGAAGAATTAAAGGAGGAAATGAATCCATTCTTCTTAGACAGCCATCCAATCCTTGAGGAAGATAACATCGTCTCACAAAAACTACATTTTCATCAACATTATTGCACCCATGCATCTTTAGTTACGAGACTGAGCGATTTGGATATGAATCAGCACATTAACAATGCCAGTTATATCAACTTCATTCTCGAGGGTACTCCTCGATCGGTGTTGGAGAAATACCAGCTCTCGGGTATAACAATCAAGTACTTAAAAGAATGTAGTGTGGACCAACAATTGCAGTCGCTTTCTGTGCCCTTAGAAAACGACGTTGTGAATGGTACTCCTCTCATCAAAGATAAAGACATCAAACTCCACCACTCAATTCTCTCTGATGGGATTGAACTTGTTCGAGCAAAAACCGTTTGGACACCAAAATTCAATTAA

Coding sequence (CDS)

ATGAGGTCCAACTCCATCAAATCTTATGGTAAGTTTGATATCATGGCACTCATATCTTCATCAAATGGCTCCCATTTTGGTATGGAATTAGTATCAATTTCAATGAGGGAACCAACATTTGGACATGCCACCACACCCAGTTTGCACCTCAAAGGCCGACCCAAAAGTGCCTCTCTTACCCTCAAATCTTCTCACATTTCTCAACCCACTGCAGGTGGGTTCGAGAGTATGGATGATTACATGTCGAGAAAACCCGAGATAACTCGTGAGAATTTAATGGTGGAAGAGTTTTTGAACGATGGTTTGGTTTACCAATGTCATTTTTTGGTGAGATCATACGAGTCGGGACCTGATAATAAGATGACAATGTTTTTCGTCATCAACCAGTTACAGGAGTCGAAACTAAATCACTTCATAAGTTGTGGATTGGTCGAAAGGGGATTGGGCAAGACAGGATTTGGCACAACACATGAAATGACTAAAAGGGACCTCATATGGGTCGTGCATAAATTGCAAGTGGTGGTCGACCAATTTCCTTCTTGGAACGACGTCGTTGAACTTCGTACGTGGATATGTAAACATGGGAAGAATAGCTATCGTCGAAACTGGATCGCTCGTGACAAGAAAAGTGGAAAAGTTTTGATCCGTGCAATTTGTTTCTATGTTTTGATGAACAGAAGAACAAGAAAATTATCCAAATTTTGTGAAGAATTAAAGGAGGAAATGAATCCATTCTTCTTAGACAGCCATCCAATCCTTGAGGAAGATAACATCGTCTCACAAAAACTACATTTTCATCAACATTATTGCACCCATGCATCTTTAGTTACGAGACTGAGCGATTTGGATATGAATCAGCACATTAACAATGCCAGTTATATCAACTTCATTCTCGAGGGTACTCCTCGATCGGTGTTGGAGAAATACCAGCTCTCGGGTATAACAATCAAGTACTTAAAAGAATGTAGTGTGGACCAACAATTGCAGTCGCTTTCTGTGCCCTTAGAAAACGACGTTGTGAATGGTACTCCTCTCATCAAAGATAAAGACATCAAACTCCACCACTCAATTCTCTCTGATGGGATTGAACTTGTTCGAGCAAAAACCGTTTGGACACCAAAATTCAATTAA

Protein sequence

MRSNSIKSYGKFDIMALISSSNGSHFGMELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN
Homology
BLAST of CmoCh08G006130 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.2e-63
Identity = 124/301 (41.20%), Postives = 191/301 (63.46%), Query Frame = 0

Query: 77  MDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLN 136
           M D+  R+P++  +   + + + DGLV+  +F +RSYE G D   ++  ++N LQE+ +N
Sbjct: 107 MLDWKPRRPDMVIDPFGIGKIVQDGLVFSQNFSIRSYEIGADQTASIETLMNHLQETAIN 166

Query: 137 HFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGK 196
           H  S GL    LG+ GFG T EM K++LIWVV ++QVVVD++P+W DVV++ TW+   GK
Sbjct: 167 HCRSAGL----LGE-GFGATPEMCKKNLIWVVTRMQVVVDRYPTWGDVVQVDTWVSASGK 226

Query: 197 NSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEED 256
           N  RR+W+  + ++G++L RA   +V+MN+ TR+LSK  EE++ E+ PFF++S P+L ED
Sbjct: 227 NGMRRDWLVSNSETGEILTRATSVWVMMNKLTRRLSKIPEEVRGEIEPFFMNSDPVLAED 286

Query: 257 NIVSQKL--HFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGI 316
           +    KL     +H C    L  + SDLD+NQH+NN  YI +ILE  P  +LE ++LS +
Sbjct: 287 SQKLVKLDDSTAEHVC--KGLTPKWSDLDVNQHVNNVKYIGWILESAPLPILESHELSAL 346

Query: 317 TIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSI-LSDGIELVRAKTVWTP 375
           T++Y +EC  D  LQSL+   +++  N    +   +    H + L DG E+VR +T W P
Sbjct: 347 TLEYRRECGRDSVLQSLTTVSDSNTENA---VNVGEFNCQHLLRLDDGAEIVRGRTRWRP 397

BLAST of CmoCh08G006130 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.4e-62
Identity = 122/299 (40.80%), Postives = 187/299 (62.54%), Query Frame = 0

Query: 77  MDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLN 136
           M D+  R+ ++  +   +   + DGLV++ +F +RSYE G D   ++  V+N LQE+ LN
Sbjct: 112 MLDWKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALN 171

Query: 137 HFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGK 196
           H  + GL+       GFG+T EM K++LIWVV ++QVVVD++P+W DVVE+ TW+ + GK
Sbjct: 172 HVKTAGLL-----GDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGK 231

Query: 197 NSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEED 256
           N  RR+W+ RD  +G+ L RA   +V+MN+ TR+LSK  EE++ E+ P+F++S P+L ED
Sbjct: 232 NGMRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAED 291

Query: 257 NIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITI 316
           +    K+         + L  R SDLD+NQH+NN  YI +ILE  P  ++E+ +L  +T+
Sbjct: 292 SRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTL 351

Query: 317 KYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSI-LSDGIELVRAKTVWTPK 375
           +Y +EC  D  LQSL+     D+ N   L    D++  H + L DG E+VR +T W+ K
Sbjct: 352 EYRRECGRDSVLQSLTAVTGCDIGN---LATAGDVECQHLLRLQDGAEVVRGRTEWSSK 402

BLAST of CmoCh08G006130 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.2e-60
Identity = 123/299 (41.14%), Postives = 182/299 (60.87%), Query Frame = 0

Query: 81  MSRKPEITRENLMVEEF-----LNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKL 140
           + RKP+  R +++ + F     + DGLV++ +F +RSYE G D   ++  V+N LQE+ L
Sbjct: 109 LDRKPK--RLDMLEDPFGLGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETAL 168

Query: 141 NHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHG 200
           NH     +   GL   GFG T EM KRDLIWVV K+QV+V+++P+W D VE+ TW+ K G
Sbjct: 169 NH-----VKTAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSG 228

Query: 201 KNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEE 260
           KN  RR+W+  D  +G++L RA   +V+MN++TRKLSK  +E++ E+ P F+DS P++E+
Sbjct: 229 KNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIED 288

Query: 261 DNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGIT 320
           D+    KL           L  R +DLD+NQH+NNA YI +ILE TP  VLE  +L  +T
Sbjct: 289 DDRKLPKLDEKSADSIRKGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLT 348

Query: 321 IKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 375
           ++Y +EC  +  L+SL+    +    G+          H   L DG E+V+ +T W PK
Sbjct: 349 LEYRRECGRESVLESLTAVDPSGEGYGSQF-------QHLLRLEDGGEIVKGRTEWRPK 393

BLAST of CmoCh08G006130 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.2e-60
Identity = 119/298 (39.93%), Postives = 180/298 (60.40%), Query Frame = 0

Query: 77  MDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLN 136
           M D+  ++P++  +   +   + DGLV++ +F +RSYE G D   ++  V+N LQE+ LN
Sbjct: 111 MLDWKPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALN 170

Query: 137 HFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGK 196
           H     +   GL   GFG T EM KRDLIWVV K+QV+V+++P+W D VE+ TW+ K GK
Sbjct: 171 H-----VKIAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGK 230

Query: 197 NSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEED 256
           N  RR+W+  D  +G++L RA   +V+MN++TR+LSK  +E++ E+ P F+DS P++E+D
Sbjct: 231 NGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSPPVIEDD 290

Query: 257 NIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITI 316
           +    KL           L  R +DLD+NQH+NN  YI +ILE TP  VLE  +L  +T+
Sbjct: 291 DRKLPKLDEKTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL 350

Query: 317 KYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 375
           +Y +EC  +  L+SL+    +    G+          H   L DG E+V+ +T W PK
Sbjct: 351 EYRRECGRESVLESLTAMDPSGGGYGSQF-------QHLLRLEDGGEIVKGRTEWRPK 396

BLAST of CmoCh08G006130 vs. ExPASy Swiss-Prot
Match: Q39473 (Dodecanoyl-[acyl-carrier-protein] hydrolase, chloroplastic OS=Cinnamomum camphora OX=13429 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.6e-56
Identity = 117/292 (40.07%), Postives = 174/292 (59.59%), Query Frame = 0

Query: 84  KPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGL 143
           KP+     L+ + F   GLV++  F +RSYE GPD   ++  V+N LQE+ LNH  S G+
Sbjct: 87  KPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSIVAVMNHLQEAALNHAKSVGI 146

Query: 144 VERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNW 203
           +       GFGTT EM+KRDLIWVV +  V V+++P+W D VE+  W+   G N  R ++
Sbjct: 147 L-----GDGFGTTLEMSKRDLIWVVKRTHVAVERYPAWGDTVEVECWVGASGNNGRRHDF 206

Query: 204 IARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKL 263
           + RD K+G++L R     V+MN RTR+LSK  EE++ E+ P F+D+  + +E+    QKL
Sbjct: 207 LVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVAVKDEEIKKPQKL 266

Query: 264 HFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECS 323
           +          L  R +DLD+NQH+NN  Y+++ILE  P S+ E + +S  TI+Y +EC+
Sbjct: 267 NDSTADYIQGGLTPRWNDLDINQHVNNIKYVDWILETVPDSIFESHHISSFTIEYRRECT 326

Query: 324 VDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSI-LSDGIELVRAKTVWTPK 375
           +D  LQSL+      V  G+    +  +   H + L  G E++RAKT W PK
Sbjct: 327 MDSVLQSLTT-----VSGGS---SEAGLVCEHLLQLEGGSEVLRAKTEWRPK 365

BLAST of CmoCh08G006130 vs. ExPASy TrEMBL
Match: A0A6J1H8Q2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461080 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.6e-203
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 28  MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 87
           MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI
Sbjct: 1   MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 60

Query: 88  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 147
           TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG
Sbjct: 61  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 120

Query: 148 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 207
           LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD
Sbjct: 121 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 180

Query: 208 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 267
           KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ
Sbjct: 181 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 240

Query: 268 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 327
           HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ
Sbjct: 241 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 300

Query: 328 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN 377
           LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN
Sbjct: 301 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN 349

BLAST of CmoCh08G006130 vs. ExPASy TrEMBL
Match: A0A6J1KZF4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497736 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.4e-191
Identity = 330/348 (94.83%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 28  MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 87
           MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQP A GF+SMDDY+SRKPEI
Sbjct: 1   MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPIASGFKSMDDYVSRKPEI 60

Query: 88  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 147
           TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLV+ G
Sbjct: 61  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTG 120

Query: 148 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 207
           LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWIC+HGKNSYRRNWIARD
Sbjct: 121 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICRHGKNSYRRNWIARD 180

Query: 208 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 267
           KKSGKVLIRAIC YVLMN+RTRKLSKFCEEL+EEMNPFFLDSHPILEEDNIVSQKLH HQ
Sbjct: 181 KKSGKVLIRAICSYVLMNKRTRKLSKFCEELEEEMNPFFLDSHPILEEDNIVSQKLHLHQ 240

Query: 268 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 327
           H CT+ASLVTRLSDLDMNQHINNASYINF+LEGTPRSVLE YQLS ITIKYLKECSVDQQ
Sbjct: 241 HDCTYASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLSRITIKYLKECSVDQQ 300

Query: 328 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF 376
           LQSLS+PLENDVVN TPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF
Sbjct: 301 LQSLSLPLENDVVNVTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF 348

BLAST of CmoCh08G006130 vs. ExPASy TrEMBL
Match: A0A5A7UZ85 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002510 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.8e-120
Identity = 227/362 (62.71%), Postives = 272/362 (75.14%), Query Frame = 0

Query: 15  MALISSSNGSHFGMELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPTAG 74
           MA +SSS   + G +  S   +E  FG + T +L LK  PK     L++S+I      A 
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 75  GFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQE 134
               ++ Y SR  +IT  NL + E LNDGLVYQ HFLV+SYESGPD KMTMFF+INQL E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 135 SKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWIC 194
           SKLNH  SCGLV      TG GTT EMTKR LIWVVHKLQVVVDQ+PSWND+V+L+TW C
Sbjct: 121 SKLNHLKSCGLV-----TTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSWNDIVQLQTWSC 180

Query: 195 KHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPI 254
            +GKNSYRR+WI R+ K+G+VLIRA CFYVLMNR TRKLSKF +E+KEEMNP FL S PI
Sbjct: 181 FYGKNSYRRDWIVRNYKTGQVLIRASCFYVLMNRNTRKLSKFYDEMKEEMNPHFLKSDPI 240

Query: 255 LEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLS 314
           +EE+NI+S KL      C   SLVTRLSDLDMN H+NNASYINFILEGTPR +LE ++LS
Sbjct: 241 IEEENIISTKLDVDAKGCNCTSLVTRLSDLDMNHHVNNASYINFILEGTPRWMLESHRLS 300

Query: 315 GITIKYLKECSVDQQLQSLSVPLENDVV-NGTPLIKDKDIKLHHSILSDGIELVRAKTVW 374
            +T+KYLKEC VD+ LQSLS+P++NDV+ NG  L+K+  IKLHHSI+S+GIELVRA T+W
Sbjct: 301 TVTVKYLKECGVDKILQSLSMPIQNDVISNGNYLVKENYIKLHHSIVSEGIELVRATTLW 357

BLAST of CmoCh08G006130 vs. ExPASy TrEMBL
Match: A0A6J1CMR0 (Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC111012889 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 7.9e-70
Identity = 136/253 (53.75%), Postives = 181/253 (71.54%), Query Frame = 0

Query: 123 MFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWN 182
           M  +INQLQES +NHF SCGLVE     TG+ +T EM KR LIWVVHKLQVVVD +PSWN
Sbjct: 1   MVSIINQLQESAINHFKSCGLVE-----TGYASTREMDKRGLIWVVHKLQVVVDLYPSWN 60

Query: 183 DVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEM 242
           D+V+L TW+C  GKN++RR+WI  D K+G+VLIRAICFY LMN+ TRKLS   +E+  ++
Sbjct: 61  DIVQLSTWVCVCGKNNFRRDWIIHDSKTGEVLIRAICFYALMNKDTRKLSNVDDEIMADI 120

Query: 243 NPFFLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINN-ASYINFILEGT 302
            PF  +  PI++EDN + +KL  +   C  + LVT+  DLD+NQH+++  SYIN+ILE  
Sbjct: 121 TPFLSNCDPIVKEDNSILRKLDVNSKDCIGSHLVTKSCDLDLNQHVSHIISYINWILESA 180

Query: 303 PRSVLEKYQLSGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDG 362
           P  V++ + LS ITIKY KEC +D++L+SL VP+ +D  NG     ++ IK  H+  +D 
Sbjct: 181 PPMVVKNHWLSAITIKYRKECGIDRKLKSLYVPIRDD-NNGDE--SEEGIKFQHAFFTDE 240

Query: 363 IELVRAKTVWTPK 375
            ELVRA+TVW PK
Sbjct: 241 CELVRARTVWKPK 245

BLAST of CmoCh08G006130 vs. ExPASy TrEMBL
Match: A0A7N2R014 (Acyl-[acyl-carrier-protein] hydrolase OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 3.4e-65
Identity = 135/305 (44.26%), Postives = 197/305 (64.59%), Query Frame = 0

Query: 77  MDDYMSRKPEITRENLMVEEF-----LNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQ 136
           M D+  R+P++   +L+++ F     + DG V++ +F +RSYE G D   ++  ++N LQ
Sbjct: 118 MLDWKPRRPDM---DLLIDPFGLGRIVQDGFVFRQNFSIRSYEIGADRTASIETLMNHLQ 177

Query: 137 ESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWI 196
           E+ LNH  + GL+       GFG+T EM K++LIWVV ++QVVVD++P+W DVV++ T +
Sbjct: 178 ETALNHVKTAGLL-----GDGFGSTPEMCKKNLIWVVARMQVVVDRYPTWGDVVQVDTSV 237

Query: 197 CKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHP 256
              GKN  RRNW  RD K+G++L RA   +V+MN++TRKLSK  EE++ E+ P+FLDS P
Sbjct: 238 GPSGKNGMRRNWFVRDYKTGEILTRASSVWVMMNKKTRKLSKIPEEVRGEIEPYFLDSDP 297

Query: 257 ILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQL 316
           ++EED+   QKL  +        L  R SDLD+NQH+NN  YI +ILE  P  +LE ++L
Sbjct: 298 VVEEDSRKLQKLDDNTADYVRTGLAPRWSDLDVNQHVNNVKYIGWILESAPLPILESHEL 357

Query: 317 SGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSI-LSDGIELVRAKTV 376
           +GIT++Y +EC  D  LQSL+    N V N   L    DI+  H + L DG E+VR +TV
Sbjct: 358 AGITLEYRRECGKDSVLQSLTAVSSNGVGN---LASLADIECQHLLRLEDGAEIVRGRTV 411

BLAST of CmoCh08G006130 vs. NCBI nr
Match: KAG6593401.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 739.6 bits (1908), Expect = 1.4e-209
Identity = 358/371 (96.50%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 6   IKSYGKFDIMALISSSNGSHFGMELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSH 65
           +KSYGKF +MALI SSNGSHFGMELVSIS REPTFGHATTPSLHLKGRPKSASLTLKSSH
Sbjct: 1   MKSYGKFAMMALICSSNGSHFGMELVSISTREPTFGHATTPSLHLKGRPKSASLTLKSSH 60

Query: 66  ISQPTAGGFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFF 125
           ISQP AGGF+S+DDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFF
Sbjct: 61  ISQPIAGGFKSIDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFF 120

Query: 126 VINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVV 185
           VINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVV
Sbjct: 121 VINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVV 180

Query: 186 ELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPF 245
           ELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMN+RTRKLS+FCEELKEEMNPF
Sbjct: 181 ELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNKRTRKLSRFCEELKEEMNPF 240

Query: 246 FLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSV 305
           FLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINF+LEGTPR+V
Sbjct: 241 FLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFVLEGTPRAV 300

Query: 306 LEKYQLSGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELV 365
           LE YQLSGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELV
Sbjct: 301 LENYQLSGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELV 360

Query: 366 RAKTVWTPKFN 377
           RAKTVWTPKFN
Sbjct: 361 RAKTVWTPKFN 371

BLAST of CmoCh08G006130 vs. NCBI nr
Match: XP_023514527.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718.8 bits (1854), Expect = 2.5e-203
Identity = 349/362 (96.41%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 15  MALISSSNGSHFGMELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGF 74
           MALI SSNGSHFGMELVSISMREPTFGHATTPSLHLK RPK+ASLTLKSSHISQP AGGF
Sbjct: 1   MALICSSNGSHFGMELVSISMREPTFGHATTPSLHLKARPKTASLTLKSSHISQPIAGGF 60

Query: 75  ESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESK 134
           +S+DDY+SRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESK
Sbjct: 61  KSIDDYVSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESK 120

Query: 135 LNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKH 194
           LNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKH
Sbjct: 121 LNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKH 180

Query: 195 GKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILE 254
           GKNSYRRNWIARDKKSGKVLIRAICFYVLMN+RTRKLSKFCEELKEEMNPFFLDSHPILE
Sbjct: 181 GKNSYRRNWIARDKKSGKVLIRAICFYVLMNKRTRKLSKFCEELKEEMNPFFLDSHPILE 240

Query: 255 EDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGI 314
           EDNIVSQKLH HQH CTHASLVTRLSDLDMNQHINNASYINF+LEGTPRSVLE YQLSGI
Sbjct: 241 EDNIVSQKLHLHQHDCTHASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLSGI 300

Query: 315 TIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 374
           TIKYLKECSVDQQLQSLSVPLENDVVNGTP IKDKDIKLHHSILSDGIELVRAKTVWTPK
Sbjct: 301 TIKYLKECSVDQQLQSLSVPLENDVVNGTPFIKDKDIKLHHSILSDGIELVRAKTVWTPK 360

Query: 375 FN 377
           FN
Sbjct: 361 FN 362

BLAST of CmoCh08G006130 vs. NCBI nr
Match: XP_022960310.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 718.4 bits (1853), Expect = 3.3e-203
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 28  MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 87
           MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI
Sbjct: 1   MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 60

Query: 88  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 147
           TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG
Sbjct: 61  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 120

Query: 148 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 207
           LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD
Sbjct: 121 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 180

Query: 208 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 267
           KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ
Sbjct: 181 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 240

Query: 268 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 327
           HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ
Sbjct: 241 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 300

Query: 328 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN 377
           LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN
Sbjct: 301 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKFN 349

BLAST of CmoCh08G006130 vs. NCBI nr
Match: XP_023004418.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 678.7 bits (1750), Expect = 2.9e-191
Identity = 330/348 (94.83%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 28  MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPTAGGFESMDDYMSRKPEI 87
           MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQP A GF+SMDDY+SRKPEI
Sbjct: 1   MELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPIASGFKSMDDYVSRKPEI 60

Query: 88  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERG 147
           TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLV+ G
Sbjct: 61  TRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTG 120

Query: 148 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARD 207
           LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWIC+HGKNSYRRNWIARD
Sbjct: 121 LGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICRHGKNSYRRNWIARD 180

Query: 208 KKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQ 267
           KKSGKVLIRAIC YVLMN+RTRKLSKFCEEL+EEMNPFFLDSHPILEEDNIVSQKLH HQ
Sbjct: 181 KKSGKVLIRAICSYVLMNKRTRKLSKFCEELEEEMNPFFLDSHPILEEDNIVSQKLHLHQ 240

Query: 268 HYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQ 327
           H CT+ASLVTRLSDLDMNQHINNASYINF+LEGTPRSVLE YQLS ITIKYLKECSVDQQ
Sbjct: 241 HDCTYASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLSRITIKYLKECSVDQQ 300

Query: 328 LQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF 376
           LQSLS+PLENDVVN TPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF
Sbjct: 301 LQSLSLPLENDVVNVTPLIKDKDIKLHHSILSDGIELVRAKTVWTPKF 348

BLAST of CmoCh08G006130 vs. NCBI nr
Match: KAA0058801.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 441.8 bits (1135), Expect = 5.9e-120
Identity = 227/362 (62.71%), Postives = 272/362 (75.14%), Query Frame = 0

Query: 15  MALISSSNGSHFGMELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPTAG 74
           MA +SSS   + G +  S   +E  FG + T +L LK  PK     L++S+I      A 
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 75  GFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQE 134
               ++ Y SR  +IT  NL + E LNDGLVYQ HFLV+SYESGPD KMTMFF+INQL E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 135 SKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWIC 194
           SKLNH  SCGLV      TG GTT EMTKR LIWVVHKLQVVVDQ+PSWND+V+L+TW C
Sbjct: 121 SKLNHLKSCGLV-----TTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSWNDIVQLQTWSC 180

Query: 195 KHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPI 254
            +GKNSYRR+WI R+ K+G+VLIRA CFYVLMNR TRKLSKF +E+KEEMNP FL S PI
Sbjct: 181 FYGKNSYRRDWIVRNYKTGQVLIRASCFYVLMNRNTRKLSKFYDEMKEEMNPHFLKSDPI 240

Query: 255 LEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLS 314
           +EE+NI+S KL      C   SLVTRLSDLDMN H+NNASYINFILEGTPR +LE ++LS
Sbjct: 241 IEEENIISTKLDVDAKGCNCTSLVTRLSDLDMNHHVNNASYINFILEGTPRWMLESHRLS 300

Query: 315 GITIKYLKECSVDQQLQSLSVPLENDVV-NGTPLIKDKDIKLHHSILSDGIELVRAKTVW 374
            +T+KYLKEC VD+ LQSLS+P++NDV+ NG  L+K+  IKLHHSI+S+GIELVRA T+W
Sbjct: 301 TVTVKYLKECGVDKILQSLSMPIQNDVISNGNYLVKENYIKLHHSIVSEGIELVRATTLW 357

BLAST of CmoCh08G006130 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 240.4 bits (612), Expect = 2.4e-63
Identity = 122/299 (40.80%), Postives = 187/299 (62.54%), Query Frame = 0

Query: 77  MDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLN 136
           M D+  R+ ++  +   +   + DGLV++ +F +RSYE G D   ++  V+N LQE+ LN
Sbjct: 112 MLDWKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALN 171

Query: 137 HFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGK 196
           H  + GL+       GFG+T EM K++LIWVV ++QVVVD++P+W DVVE+ TW+ + GK
Sbjct: 172 HVKTAGLL-----GDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGK 231

Query: 197 NSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEED 256
           N  RR+W+ RD  +G+ L RA   +V+MN+ TR+LSK  EE++ E+ P+F++S P+L ED
Sbjct: 232 NGMRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAED 291

Query: 257 NIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITI 316
           +    K+         + L  R SDLD+NQH+NN  YI +ILE  P  ++E+ +L  +T+
Sbjct: 292 SRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTL 351

Query: 317 KYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSI-LSDGIELVRAKTVWTPK 375
           +Y +EC  D  LQSL+     D+ N   L    D++  H + L DG E+VR +T W+ K
Sbjct: 352 EYRRECGRDSVLQSLTAVTGCDIGN---LATAGDVECQHLLRLQDGAEVVRGRTEWSSK 402

BLAST of CmoCh08G006130 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 167.2 bits (422), Expect = 2.6e-41
Identity = 99/296 (33.45%), Postives = 153/296 (51.69%), Query Frame = 0

Query: 90  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLG 149
           + L +     DGL Y+  F+VRSYE G +   T+  + N LQE   NH  S      G  
Sbjct: 70  DQLRLGSLTEDGLSYKEKFVVRSYEVGSNKTATVETIANLLQEVGCNHAQSV-----GFS 129

Query: 150 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKK 209
             GF TT  M K  LIWV  ++ + + ++P+W DVVE+ TW    G+   RR+WI +D  
Sbjct: 130 TDGFATTTTMRKLHLIWVTARMHIEIYKYPAWGDVVEIETWCQSEGRIGTRRDWILKDSV 189

Query: 210 SGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPIL---EEDNIVSQKLHFH 269
           +G+V  RA   +V+MN+ TR+L K  +++++E    F    P L   EE+N   +K+   
Sbjct: 190 TGEVTGRATSKWVMMNQDTRRLQKVSDDVRDEY-LVFCPQEPRLAFPEENNRSLKKIPKL 249

Query: 270 QHYCTHA--SLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSV 329
           +    ++   L  R +DLDMNQH+NN +YI ++LE  P+ +++ ++L  IT+ Y +EC  
Sbjct: 250 EDPAQYSMIGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIVDTHELQVITLDYRRECQQ 309

Query: 330 DQQLQSLSVP------LENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 375
           D  + SL+              +GT    D        +  DG E+ R  T+W  K
Sbjct: 310 DDVVDSLTTTTSEIGGTNGSATSGTQGHNDSQFLHLLRLSGDGQEINRGTTLWRKK 359

BLAST of CmoCh08G006130 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 164.5 bits (415), Expect = 1.7e-40
Identity = 99/302 (32.78%), Postives = 160/302 (52.98%), Query Frame = 0

Query: 90  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLG 149
           + L     + DG  Y+  F+VRSYE G +   T+  + N LQE   NH     +   G  
Sbjct: 69  DRLRFGRLMEDGFSYKEKFIVRSYEVGINKTATIETIANLLQEVACNH-----VQNVGFS 128

Query: 150 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKK 209
             GF TT  M K  LIWV  ++ + + ++P+W+DVVE+ TW    G+   RR+WI +D  
Sbjct: 129 TDGFATTLTMRKLHLIWVTARMHIEIYKYPAWSDVVEIETWCQSEGRIGTRRDWILKDCA 188

Query: 210 SGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPIL---EEDNIVSQKLHFH 269
           +G+V+ RA   +V+MN+ TR+L +  +E+++E    F    P L   EE+N   +K+   
Sbjct: 189 TGEVIGRATSKWVMMNQDTRRLQRVTDEVRDEY-LVFCPPEPRLAFPEENNSSLKKIPKL 248

Query: 270 QHYCTHA--SLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSV 329
           +    ++   L  R +DLDMNQH+NN +YI ++LE  P+ +++ ++L  IT+ Y +EC  
Sbjct: 249 EDPAQYSMLGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIIDTHELKVITLDYRRECQQ 308

Query: 330 DQQLQSLSV-PLENDVV------NGTPLIKDKDIKLHH-----SILSDGIELVRAKTVWT 375
           D  + SL+     N+VV      NG+     ++    H      +  +G E+ R +T W 
Sbjct: 309 DDIVDSLTTSETPNEVVSKLTGTNGSTTSSKREHNESHFLHILRLSENGQEINRGRTQWR 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQI35.2e-6341.20Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum... [more]
Q9SJE23.4e-6240.80Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia... [more]
G3ESV04.2e-6041.14Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ... [more]
Q395134.2e-6039.93Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ... [more]
Q394733.6e-5640.07Dodecanoyl-[acyl-carrier-protein] hydrolase, chloroplastic OS=Cinnamomum camphor... [more]
Match NameE-valueIdentityDescription
A0A6J1H8Q21.6e-203100.00Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461... [more]
A0A6J1KZF41.4e-19194.83Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149773... [more]
A0A5A7UZ852.8e-12062.71Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1CMR07.9e-7053.75Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A7N2R0143.4e-6544.26Acyl-[acyl-carrier-protein] hydrolase OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6593401.11.4e-20996.50Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
XP_023514527.12.5e-20396.41palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo ... [more]
XP_022960310.13.3e-203100.00palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita mosch... [more]
XP_023004418.12.9e-19194.83palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxim... [more]
KAA0058801.15.9e-12062.71palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596... [more]
Match NameE-valueIdentityDescription
AT1G08510.12.4e-6340.80fatty acyl-ACP thioesterases B [more]
AT3G25110.12.6e-4133.45fatA acyl-ACP thioesterase [more]
AT4G13050.11.7e-4032.78Acyl-ACP thioesterase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002864Acyl-ACP thioesterasePFAMPF01643Acyl-ACP_TEcoord: 101..372
e-value: 1.5E-53
score: 181.7
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 95..375
e-value: 2.1E-72
score: 245.4
NoneNo IPR availablePANTHERPTHR31727:SF2PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTICcoord: 70..375
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 70..375
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 274..371
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 100..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G006130.1CmoCh08G006130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016297 acyl-[acyl-carrier-protein] hydrolase activity
molecular_function GO:0016790 thiolester hydrolase activity