CmoCh08G004900 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G004900
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDelta(24)-sterol reductase
LocationCmo_Chr08: 3020083 .. 3023184 (+)
RNA-Seq ExpressionCmoCh08G004900
SyntenyCmoCh08G004900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCATCTTCATTCTCTGTTTGCAACTTTTTTTCTCGGCTTTTACACACGCCTTTACGCTTTGATTTCTTTGTTCCTCTGTTTCACATTTATTCCTCGCCGGTAACCTCTCACCTTCTTCGCCCAAACTGCTACTGAATGCTGATGCAACTTTTTGATCCTTCATTTTCGTGTTTGTGAATGAGTGATCTTAGTGCTGCTTTCTCTGTTTTTCCTCTGTTTGGTTGCTGAGAAATTGAGTGAGAATCCACTCCTCTAATTTTATATTTTATTCTTTTATCTGTTCCAGATACCAGATACCGTTGCACGAGGAATTTAGTTTCGTCCCTTCTTTTTTTTTTTTTTTTCTCTCTGAGCAAAAAAATATTAGGTTGATTGTATCTACTCTTGGAATAATTGCTTAAAACGCCTGCATAAATAAGTTTTTGTGTCTGATGAATCATAAATAAGTTCTTGTTTCTGATGAATGAACATGATATGCGGTTGAATAGACTATATGGGCTTTCCTTCCTCTAAAATGCTGAAATGCATTTGAACACTCCTTTTGTTTCTGTTTTTTTCAATGTCACTTAATAAACCCTCTTTTTTTTTTCTCGGTAGTGGCGTACAATCTTTTTGTAGAGGCCATTCTTGATGAAACTTCACTCTTGATTTAAAAATGTGAGATATTTTTGCAACCATTTTGACCATTTGTCTTCATTGTTACAGCATTGAAGGCGTAATCGTGTAGCACAATGTCAGATTTAGAGGTTCCTTTGCGTCCAAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTGAAATTCCGATGGATTTTAGTTATTTTTGTTGTCCTTCCTATATCATTCTGCCTATACTTCTTCACATATCTTGGAGATGTCAGATCTGCCTGGAAGTCCTACAAGAAACGCCAAAAGGAACATGATGAAAATGTCGAGAAAGTTGTAAAGCGCCTTAAACAGAGGAACCCAGCAAAGGATGGACTTGTTTGCACAGCTCGAAAACCATGGATTGCTGTCGGAATGCGAAATGTCGACTATAAAAGAGCACGCCATTTTGAGGTTGATTTATCAGCTTTTAGGAACATTTTGGATATTGACCAAGAGAAAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGACAAATCAGTAGAGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTTGCTTACGAGGTTGTTCTAGCTGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCGATCCCATGGTCCCAAGGAACAATTGGCCTTCTTGTTGCTGCTGAGATTAAGCTTATACCAATTAAGGAGTATATGAAAGTTACATATAAACCTTTTAGAGGGACTTTAAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCACCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCTGACTTTGTTGAAACTATGATTTACACTCCTTCTGAAGCTGTTGTCATGACAGGTAGATATGCCTCCAAAGTAGAGGCCAAGAGAAAGGGAAATGTGATCAATCAAATTGGATGGTGGTTCAAACCGTGGTTCTATCAGCATGCATCTACAGCACTCAAGAAAGGGGAGTTTGTGGAGTATATTCCTACAAGGGATTATTATCATAGGCACACAAGGTCTTTGTATTGGGAGGGTAAGCTTATTCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTTGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGTGAAGCTATCAGGAATTATTATCATGAAATGCATGTTATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTGGAGGTACTGCTCTTCATCTTATACATTCATAGTTATCATTGAACTTCAAAGTTATCCAATACCATCAAATGCTCAACTTTGTGTTTTGAAATACAAATCCCTTGTCACAAATCTAATAGGATAAAATCCATAAAATTCTAAAAAAATATCTAACACGTTAGAGATCTATGAAACACAAAATTGACTTGTGATATAAGGGATAAAATTGATTTGTATTTTTACTAGAGGTGGTTATAGGCAAGTGGCTGATGAGTCTATCTGAAGTGGATTTCAATTGAAGATGCAGATTTGGTTAACAACAGCAATTTGTTGGTGTGGTCTTGCACAGGTTTACCCCGTTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGAGACACGCATTACGCTCAAATGTACACAGATGTCGGGCTGTACTATGCACCAGGCCCTATCCTGAGGGGTGAAGTTTTCGATGGCGCCGAAGCTGTTCGTAGAATGGAGAAATGGCTTCTGGAAAACCATGGATTCCAAGCTCTATATGCAGTTTCAGAACTGAATGAGAAGGATTTCTGGAGGATGTTTGACGCAGAACACTATGAGCAATGCAGGAAGAAGTATGGCGCTGTAGGAACATTTATGACAGTGTATTACAAGTCCAAGAAAGGTCGGAAAACTGAGAAGGAAGTGAAGGAAGCTGAACAAGCTCATCTTGAAACCACTTATGCTGAACTGGAACAACCCAATTAAATCAACTTCCAGCTTGAAATATCTGGTGGGTTTTCTTATTTAATCCTCTTTTATTTTCCTGTTGACATTTCTTTTTTTCTTTTTTAGAATTTTGAATGATGCTTTTGTAGTTTTTTTTCATGTCTGATCTGATCTGAGTTGCCTGTCTGACCCGCCTAGGTTGATTTTAAATTTGAAAATTGTTGTAGTTTGAACAGCCGAACTTGATGGATTATCATCAGAGTTTGTCTTTGATGCTTTGTTTTATGAAATAGATGAGATGTTGATGAGGTGATTAGTGTTTGATGAAGATTTGTAATAAAAATGCTGGTCTTCATGGATATGTTAGGAAAGGAATTTATGGA

mRNA sequence

TTCATCTTCATTCTCTGTTTGCAACTTTTTTTCTCGGCTTTTACACACGCCTTTACGCTTTGATTTCTTTGTTCCTCTGTTTCACATTTATTCCTCGCCGGTAACCTCTCACCTTCTTCGCCCAAACTGCTACTGAATGCTGATGCAACTTTTTGATCCTTCATTTTCGTGTTTGTGAATGAGTGATCTTAGTGCTGCTTTCTCTGTTTTTCCTCTGTTTGGTTGCTGAGAAATTGACATTGAAGGCGTAATCGTGTAGCACAATGTCAGATTTAGAGGTTCCTTTGCGTCCAAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTGAAATTCCGATGGATTTTAGTTATTTTTGTTGTCCTTCCTATATCATTCTGCCTATACTTCTTCACATATCTTGGAGATGTCAGATCTGCCTGGAAGTCCTACAAGAAACGCCAAAAGGAACATGATGAAAATGTCGAGAAAGTTGTAAAGCGCCTTAAACAGAGGAACCCAGCAAAGGATGGACTTGTTTGCACAGCTCGAAAACCATGGATTGCTGTCGGAATGCGAAATGTCGACTATAAAAGAGCACGCCATTTTGAGGTTGATTTATCAGCTTTTAGGAACATTTTGGATATTGACCAAGAGAAAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGACAAATCAGTAGAGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTTGCTTACGAGGTTGTTCTAGCTGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCGATCCCATGGTCCCAAGGAACAATTGGCCTTCTTGTTGCTGCTGAGATTAAGCTTATACCAATTAAGGAGTATATGAAAGTTACATATAAACCTTTTAGAGGGACTTTAAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCACCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCTGACTTTGTTGAAACTATGATTTACACTCCTTCTGAAGCTGTTGTCATGACAGGTAGATATGCCTCCAAAGTAGAGGCCAAGAGAAAGGGAAATGTGATCAATCAAATTGGATGGTGGTTCAAACCGTGGTTCTATCAGCATGCATCTACAGCACTCAAGAAAGGGGAGTTTGTGGAGTATATTCCTACAAGGGATTATTATCATAGGCACACAAGGTCTTTGTATTGGGAGGGTAAGCTTATTCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTTGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGTGAAGCTATCAGGAATTATTATCATGAAATGCATGTTATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTGGAGGTTTACCCCGTTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGAGACACGCATTACGCTCAAATGTACACAGATGTCGGGCTGTACTATGCACCAGGCCCTATCCTGAGGGGTGAAGTTTTCGATGGCGCCGAAGCTGTTCGTAGAATGGAGAAATGGCTTCTGGAAAACCATGGATTCCAAGCTCTATATGCAGTTTCAGAACTGAATGAGAAGGATTTCTGGAGGATGTTTGACGCAGAACACTATGAGCAATGCAGGAAGAAGTATGGCGCTGTAGGAACATTTATGACAGTGTATTACAAGTCCAAGAAAGGTCGGAAAACTGAGAAGGAAGTGAAGGAAGCTGAACAAGCTCATCTTGAAACCACTTATGCTGAACTGGAACAACCCAATTAAATCAACTTCCAGCTTGAAATATCTGGTGGGTTTTCTTATTTAATCCTCTTTTATTTTCCTGTTGACATTTCTTTTTTTCTTTTTTAGAATTTTGAATGATGCTTTTGTAGTTTTTTTTCATGTCTGATCTGATCTGAGTTGCCTGTCTGACCCGCCTAGGTTGATTTTAAATTTGAAAATTGTTGTAGTTTGAACAGCCGAACTTGATGGATTATCATCAGAGTTTGTCTTTGATGCTTTGTTTTATGAAATAGATGAGATGTTGATGAGGTGATTAGTGTTTGATGAAGATTTGTAATAAAAATGCTGGTCTTCATGGATATGTTAGGAAAGGAATTTATGGA

Coding sequence (CDS)

ATGTCAGATTTAGAGGTTCCTTTGCGTCCAAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTGAAATTCCGATGGATTTTAGTTATTTTTGTTGTCCTTCCTATATCATTCTGCCTATACTTCTTCACATATCTTGGAGATGTCAGATCTGCCTGGAAGTCCTACAAGAAACGCCAAAAGGAACATGATGAAAATGTCGAGAAAGTTGTAAAGCGCCTTAAACAGAGGAACCCAGCAAAGGATGGACTTGTTTGCACAGCTCGAAAACCATGGATTGCTGTCGGAATGCGAAATGTCGACTATAAAAGAGCACGCCATTTTGAGGTTGATTTATCAGCTTTTAGGAACATTTTGGATATTGACCAAGAGAAAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGACAAATCAGTAGAGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTTGCTTACGAGGTTGTTCTAGCTGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCGATCCCATGGTCCCAAGGAACAATTGGCCTTCTTGTTGCTGCTGAGATTAAGCTTATACCAATTAAGGAGTATATGAAAGTTACATATAAACCTTTTAGAGGGACTTTAAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCACCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCTGACTTTGTTGAAACTATGATTTACACTCCTTCTGAAGCTGTTGTCATGACAGGTAGATATGCCTCCAAAGTAGAGGCCAAGAGAAAGGGAAATGTGATCAATCAAATTGGATGGTGGTTCAAACCGTGGTTCTATCAGCATGCATCTACAGCACTCAAGAAAGGGGAGTTTGTGGAGTATATTCCTACAAGGGATTATTATCATAGGCACACAAGGTCTTTGTATTGGGAGGGTAAGCTTATTCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTTGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGTGAAGCTATCAGGAATTATTATCATGAAATGCATGTTATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTGGAGGTTTACCCCGTTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGAGACACGCATTACGCTCAAATGTACACAGATGTCGGGCTGTACTATGCACCAGGCCCTATCCTGAGGGGTGAAGTTTTCGATGGCGCCGAAGCTGTTCGTAGAATGGAGAAATGGCTTCTGGAAAACCATGGATTCCAAGCTCTATATGCAGTTTCAGAACTGAATGAGAAGGATTTCTGGAGGATGTTTGACGCAGAACACTATGAGCAATGCAGGAAGAAGTATGGCGCTGTAGGAACATTTATGACAGTGTATTACAAGTCCAAGAAAGGTCGGAAAACTGAGAAGGAAGTGAAGGAAGCTGAACAAGCTCATCTTGAAACCACTTATGCTGAACTGGAACAACCCAATTAA

Protein sequence

MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQKEHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRKGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPVWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEAVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKGRKTEKEVKEAEQAHLETTYAELEQPN
Homology
[Close]
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match: P93472 (Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 2.6e-292
Identity = 472/565 (83.54%), Postives = 521/565 (92.21%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLE PLRPKRKK+WVDYFVKFRWILVIFVVLPISF LYF TYLGDVRS WKS+K RQK
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVV RLK+RNP+KDGLVCTARKPW+AVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+E+MIA+VEPLVNMGQI+R TVP+NL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVA+E++LADG +V+ATKDNEYSDLFYAIPWSQGT+GLLVAAE+KLIPIKEYMK+TYK
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           P  G L++I QAY DS APRDGDQDN EKVPDFVETMIY+P+ AV MTGRYASK EAK+K
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GN IN +GWW+K WFYQHA TALKKG FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKV DALEW+ RE+E+YP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPH+LFKLP+KTMIYPE GFEL RRQGDT  AQM+TDVG+YYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR+ME W++ENH FQ  YAVSELNEK+FWRMFDA  YE CR+KYGAVGTFM+VYYK KKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQP 566
           RKTEKEV+EAEQAHL+T Y E++QP
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQP 565

[Close]
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match: Q39085 (Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2)

HSP 1 Score: 938.3 bits (2424), Expect = 3.9e-272
Identity = 441/564 (78.19%), Postives = 501/564 (88.83%), Query Frame = 0 

Query: 1   MSDLEVPL-RPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFVKFRWI+VIF+VLP S   YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENV+KV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++EKM A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYE+VLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKR 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASK EAK+
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+L GW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 VWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAE 480
           +WLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG +LRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ E YE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELE 564
           GRKTEKEV+EAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

[Close]
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match: Q5BQE6 (Delta(24)-sterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr24 PE=2 SV=2)

HSP 1 Score: 386.0 bits (990), Expect = 7.5e-106
Identity = 217/523 (41.49%), Postives = 311/523 (59.46%), Query Frame = 0 

Query: 24  RWILVIFVVLPISFCLYFFTYLGDVRSAWKSYK--KRQKEHDENVEKVVKRLKQ-RNPAK 83
           RW+ V   +LP+S     + Y+     AW  +K     + H++ V+ + K++++ +    
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV----RAWVVFKLSSAPRLHEQRVQDIQKQVREWKEQGS 90

Query: 84  DGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDQEKMIAKVEPLVNMGQISR 143
              +CT R  W+ V +R   YK+  H  + ++   +IL++D +K I +VEPLV+MGQ++ 
Sbjct: 91  KTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVSMGQVTA 150

Query: 144 ATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVVRATK 203
               +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++LADG  VR T 
Sbjct: 151 LLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRCT- 210

Query: 204 DNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLAPR 263
            +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P RG      +A  +     
Sbjct: 211 PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICEKFTHE 270

Query: 264 DGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRKGNVINQIGWWFKPWFYQHAS 323
               +N      FVE ++Y+  EAV+MTG     VE  +    +N IG ++KPWF++H  
Sbjct: 271 SQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSYYKPWFFKHVE 330

Query: 324 TALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLFGWMMPPKVSLLKAT 383
             LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+LFGWM+PPK+SLLK T
Sbjct: 331 NYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLT 390

Query: 384 QGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPVWLCPHRLFKLPVKTMIYPE 443
           QGE +R  Y + HV+QDMLVP+  +  AL     ++ VYP+WLCP   F LP       +
Sbjct: 391 QGETLRKLYEQHHVVQDMLVPMKCLSQALHTFQNDIHVYPIWLCP---FILP------SQ 450

Query: 444 PGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEAVRRMEKWLLENHGFQALYA 503
           PG  L   +GD   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ LYA
Sbjct: 451 PG--LVHPKGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQMLYA 510

Query: 504 VSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKGRK 543
              +N ++FW MFD   Y + RK+ G    F  VY K  K  +
Sbjct: 511 DCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

[Close]
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match: Q8VCH6 (Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.3e-105
Identity = 217/523 (41.49%), Postives = 310/523 (59.27%), Query Frame = 0 

Query: 24  RWILVIFVVLPISFCLYFFTYLGDVRSAWKSYK--KRQKEHDENVEKVVKRLKQ-RNPAK 83
           RW+ V   +LP+S     + Y+     AW  +K     + H++ V  + K++++ +    
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV----RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGS 90

Query: 84  DGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDQEKMIAKVEPLVNMGQISR 143
              +CT R  W+ V +R   YK+  H  + ++   +IL++D +K I +VEPLV+MGQ++ 
Sbjct: 91  KTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVSMGQVTA 150

Query: 144 ATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVVRATK 203
               +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++LADG  VR T 
Sbjct: 151 LLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRCT- 210

Query: 204 DNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLAPR 263
            +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P RG      +A  +     
Sbjct: 211 PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICEKFTRE 270

Query: 264 DGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRKGNVINQIGWWFKPWFYQHAS 323
               +N      FVE ++Y+  EAV+MTG     VE  +    +N IG ++KPWF++H  
Sbjct: 271 SQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSYYKPWFFKHVE 330

Query: 324 TALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLFGWMMPPKVSLLKAT 383
             LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+LFGWM+PPK+SLLK T
Sbjct: 331 NYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLT 390

Query: 384 QGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPVWLCPHRLFKLPVKTMIYPE 443
           QGE +R  Y + HV+QDMLVP+  +  AL     ++ VYP+WLCP   F LP       +
Sbjct: 391 QGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCP---FILP------SQ 450

Query: 444 PGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEAVRRMEKWLLENHGFQALYA 503
           PG  L   +GD   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ LYA
Sbjct: 451 PG--LVHPKGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQMLYA 510

Query: 504 VSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKGRK 543
              +N ++FW MFD   Y + RK+ G    F  VY K  K  +
Sbjct: 511 DCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

[Close]
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match: Q15392 (Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 384.8 bits (987), Expect = 1.7e-105
Identity = 216/523 (41.30%), Postives = 309/523 (59.08%), Query Frame = 0 

Query: 24  RWILVIFVVLPISFCLYFFTYLGDVRSAWKSYK--KRQKEHDENVEKVVKRLKQ-RNPAK 83
           RW+ V   +LP+S     + Y+     AW  +K     + H++ V  + K++++ +    
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV----RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGS 90

Query: 84  DGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDQEKMIAKVEPLVNMGQISR 143
              +CT R  W+ V +R   YK+  H  + ++   +IL++D +K I +VEPLV MGQ++ 
Sbjct: 91  KTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQVTA 150

Query: 144 ATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVVRATK 203
               +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR T 
Sbjct: 151 LLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCT- 210

Query: 204 DNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLAPR 263
            +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P RG      +A        
Sbjct: 211 PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICAKFTHE 270

Query: 264 DGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRKGNVINQIGWWFKPWFYQHAS 323
              Q+N      FVE ++Y+  EAV+MTG    + E  +    +N IG ++KPWF++H  
Sbjct: 271 SQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNYYKPWFFKHVE 330

Query: 324 TALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLFGWMMPPKVSLLKAT 383
             LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+LFGWM+PPK+SLLK T
Sbjct: 331 NYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLT 390

Query: 384 QGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPVWLCPHRLFKLPVKTMIYPE 443
           QGE +R  Y + HV+QDMLVP+  +  AL     ++ VYP+WLCP   F LP       +
Sbjct: 391 QGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILP------SQ 450

Query: 444 PGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEAVRRMEKWLLENHGFQALYA 503
           PG  +H +  +   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ LYA
Sbjct: 451 PGL-VHPKGNE---AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQMLYA 510

Query: 504 VSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKGRK 543
              +N ++FW MFD   Y + R+K G    F  VY K  K  +
Sbjct: 511 DCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

[Close]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match: A0A6J1H6V4 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV=1)

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match: A0A6J1KZQ6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 565/566 (99.82%), Postives = 566/566 (100.00%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGE+FDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEIFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match: A0A6J1JUW6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 545/566 (96.29%), Postives = 558/566 (98.59%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+E+MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLA+GQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM GRYASK EAK K
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMKGRYASKEEAKIK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEY+PTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYVPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match: A0A6J1GQ50 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 544/566 (96.11%), Postives = 558/566 (98.59%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+E+MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQ+VRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASK EAK+K
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETT AELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTCAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match: A0A1S3CE88 (Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499976 PE=4 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 531/566 (93.82%), Postives = 553/566 (97.70%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL VPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLNVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGL+CTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVVKRLKQRNPAKDGLICTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+++MIAKVEPLVNMGQISRATVPLNL+LAVV ELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKDRMIAKVEPLVNMGQISRATVPLNLALAVVPELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLI +KEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIHVKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASK EAK+K
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMH+IQDML+PLYKVPDALEWAHRE EVYPV
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLIPLYKVPDALEWAHREFEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHR+FKLPVKTMI+PEPGFEL  RQGDT YAQMYTDVGLYYAPGP+LRGE FDGAEA
Sbjct: 421 WLCPHRIFKLPVKTMIFPEPGFELQSRQGDTKYAQMYTDVGLYYAPGPVLRGEAFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFW+M+DA  YEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWKMYDAGLYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAE+EQPN
Sbjct: 541 RKTEKEVREAEQAHLETTYAEMEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. NCBI nr
Match: XP_022960164.1 (delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960165.1 delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960166.1 delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960167.1 delta(24)-sterol reductase-like [Cucurbita moschata] >KAG7025620.1 Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. NCBI nr
Match: XP_023004499.1 (delta(24)-sterol reductase-like [Cucurbita maxima] >XP_023004500.1 delta(24)-sterol reductase-like [Cucurbita maxima] >XP_023004501.1 delta(24)-sterol reductase-like [Cucurbita maxima] >XP_023004502.1 delta(24)-sterol reductase-like [Cucurbita maxima] >XP_023004503.1 delta(24)-sterol reductase-like [Cucurbita maxima])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 565/566 (99.82%), Postives = 566/566 (100.00%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGE+FDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEIFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. NCBI nr
Match: XP_023514607.1 (delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023514608.1 delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023514609.1 delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023514610.1 delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 561/566 (99.12%), Postives = 564/566 (99.65%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIP+KEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPVKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PFRGTL+EIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK
Sbjct: 241 PFRGTLKEIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELH RQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHHRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYE CRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEYCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLET+YAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETSYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. NCBI nr
Match: KAG7014278.1 (Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 545/566 (96.29%), Postives = 559/566 (98.76%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+E+MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGL+NGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLVNGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASK EAK+K
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. NCBI nr
Match: XP_023548025.1 (delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548028.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548029.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548030.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548031.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548032.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548033.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548034.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548035.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548036.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548037.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 546/566 (96.47%), Postives = 559/566 (98.76%), Query Frame = 0 

Query: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+E+MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK+K
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILP ADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPLADQWW 360

Query: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDAL+WAHRELEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALDWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELEQPN 567
           RKTEKEVKEAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

[Close]
BLAST of CmoCh08G004900 vs. TAIR 10
Match: AT3G19820.1 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 938.3 bits (2424), Expect = 2.8e-273
Identity = 441/564 (78.19%), Postives = 501/564 (88.83%), Query Frame = 0 

Query: 1   MSDLEVPL-RPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFVKFRWI+VIF+VLP S   YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENV+KV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++EKM A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYE+VLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKR 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASK EAK+
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+L GW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 VWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAE 480
           +WLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG +LRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ E YE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELE 564
           GRKTEKEV+EAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

[Close]
BLAST of CmoCh08G004900 vs. TAIR 10
Match: AT3G19820.2 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 938.3 bits (2424), Expect = 2.8e-273
Identity = 441/564 (78.19%), Postives = 501/564 (88.83%), Query Frame = 0 

Query: 1   MSDLEVPL-RPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFVKFRWI+VIF+VLP S   YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENV+KV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++EKM A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYE+VLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKR 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASK EAK+
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+L GW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 VWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAE 480
           +WLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG +LRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ E YE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELE 564
           GRKTEKEV+EAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

[Close]
BLAST of CmoCh08G004900 vs. TAIR 10
Match: AT3G19820.3 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 938.3 bits (2424), Expect = 2.8e-273
Identity = 441/564 (78.19%), Postives = 501/564 (88.83%), Query Frame = 0 

Query: 1   MSDLEVPL-RPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFVKFRWI+VIF+VLP S   YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENV+KV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++EKM A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYE+VLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKR 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASK EAK+
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+L GW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 VWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAE 480
           +WLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG +LRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ E YE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELE 564
           GRKTEKEV+EAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

[Close]
BLAST of CmoCh08G004900 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 50.8 bits (120), Expect = 4.1e-06
Identity = 41/144 (28.47%), Postives = 67/144 (46.53%), Query Frame = 0 

Query: 138 ISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVVR 197
           I R T+   LS     +   LTVGG ++  GI G +  +G   + V   E+V   G+VV 
Sbjct: 170 ILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVT 229

Query: 198 ATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIP---IKEYMKVTYKPFRGTLREIGQAYM 257
            + +   S+LF+++    G  G++  A I L P   + ++++V Y  F    R+  Q Y+
Sbjct: 230 CS-EKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRD--QEYL 289

Query: 258 DSLAPRDGDQDNPEKVPDFVETMI 279
            S           EK  D+VE  +
Sbjct: 290 IS----------KEKTFDYVEGFV 300

[Close]
BLAST of CmoCh08G004900 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 49.7 bits (117), Expect = 9.1e-06
Identity = 29/92 (31.52%), Postives = 47/92 (51.09%), Query Frame = 0 

Query: 137 QISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEVVLADGQVV 196
           ++   T+ L L+     +   LTVGG ++  GI G +  YG     V+  +V+   G++ 
Sbjct: 150 EVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIA 209

Query: 197 RATKDNEYSDLFYAIPWSQGTIGLLVAAEIKL 229
             +KD   SDLF+A+    G  G++  A IKL
Sbjct: 210 TCSKDMN-SDLFFAVLGGLGQFGIITRARIKL 240

The following BLAST results are available for this feature:
BLAST of CmoCh08G004900 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P934722.6e-29283.54Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1[more]
Q390853.9e-27278.19Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2[more]
Q5BQE67.5e-10641.49Delta(24)-sterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr24 PE=2 SV=2[more]
Q8VCH61.3e-10541.49Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1[more]
Q153921.7e-10541.30Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2[more]
BLAST of CmoCh08G004900 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H6V40.0e+00100.00Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV... [more]
A0A6J1KZQ60.0e+0099.82Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1[more]
A0A6J1JUW60.0e+0096.29Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1[more]
A0A6J1GQ500.0e+0096.11Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV... [more]
A0A1S3CE880.0e+0093.82Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499976 PE=4 SV=1[more]
BLAST of CmoCh08G004900 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022960164.10.0e+00100.00delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960165.1 delta(24)-s... [more]
XP_023004499.10.0e+0099.82delta(24)-sterol reductase-like [Cucurbita maxima] >XP_023004500.1 delta(24)-ste... [more]
XP_023514607.10.0e+0099.12delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023514608.1 del... [more]
KAG7014278.10.0e+0096.29Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023548025.10.0e+0096.47delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24... [more]
BLAST of CmoCh08G004900 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G19820.12.8e-27378.19cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.22.8e-27378.19cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.32.8e-27378.19cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT2G41510.14.1e-0628.47cytokinin oxidase/dehydrogenase 1 [more]
AT5G56970.19.1e-0631.52cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 109..198
e-value: 3.7E-15
score: 55.9
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 110..231
e-value: 1.7E-26
score: 94.3
NoneNo IPR availablePANTHERPTHR10801:SF16S-LOCUS GLYCOPROTEIN DOMAIN, BULB-TYPE LECTIN DOMAIN, FAD-BINDING, TYPE 2-RELATEDcoord: 1..564
IPR040165Delta(24)-sterol reductasePANTHERPTHR1080124-DEHYDROCHOLESTEROL REDUCTASEcoord: 1..564
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 45..231
score: 13.392434
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 105..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G004900.1CmoCh08G004900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050614 delta24-sterol reductase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity