CmoCh08G004020 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G004020
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionautophagy-related protein 18a-like
LocationCmo_Chr08: 2496145 .. 2499125 (+)
RNA-Seq ExpressionCmoCh08G004020
SyntenyCmoCh08G004020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTATAAATCATCGCCATTATCTTCGTTTTCATCTGAATCTCGAGTTCCTCTGATTCTCGATGGAGACTCTCTCCGCCCTCCCTTCCTCTTCATGGCCGGAATCCAACTCCAATCCCAACACTCCGCCTGCTATTGCCGACTCCGAACGCTTTGATTCTGATTCCGTTTCGTCGTTGCCGCCTCAATCGCCGCAGACAGTGGAGACGGCTGGTTTGTCCACGGCGGCGGTTGATTCGCTCGCTTTGCAGCCTTCTCTTCTTCATCTCTCATTCAATCAAGACCATGGCTGCTTTGCTGCGGGCAGTGATCGAGGGTTTCGGATCTACAATTGCGATCCGTTCCGTGAGATTTTCAGAAGAGATTTTGAGCGTGGGGGTGGTATTGGCGTTGTGGAGATGCTTTTTCGGTGTAATATTTTGGCTCTTGTTGGTGGTGGACCTGAACCTCAGTACCCGCCTAATAAGGTCATGCTTTGGGATGATCATCAGAGCCGGTGTATTGGGGAGCTGTCGTTTCGGTCTGCTGTTCGGGGGGTTCGGCTACAGAGAGACCGAATTATTGTTATTTTGGAGCAGAAGGTTTTTGTGTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAATCCTAAAGGGCTTTGTGCTGTTTCTCAGCTGAGTTCTTCGCTTGTGCTTGTCTGCCCTGGATTGATTAAGGGGCAGGTTAGGGTTGAGCATTATGCCTCTCGACGGACAAAGTTTATCATGGCTCACGACTCGAGGGTTGCTTGCTTTGCGCTTACGACCAACGGGCAGTTGCTTGCTACAGCGAGCTCCAAGGGAACTTTGGTTCGGATATTCAATACAATTGATGGAAATTTGCTCCAAGAGGTGTGTTCATCTTGGAATTAAAATTGTTCATCTGGGTTTGTTCTGTGTTGTCGGAATCTCAGTGTAATGTTGTTTTTCTGAACTAAGCTGCGATCTAATTCTCCCCATTTAGTTTTGAGTTTGTAGGAATTTACATGACTTCTTTATGAAGTATCGAATTTGGCAGTGAATTTACTTTGTATTATCTTGGTATGATGTATAGGTACGGAGAGGCGCAGATAGGGCGGAGATATACAGTTTGGCCTTCTCATCGACTGCTCAATGGCTGGCAGTCTCCAGTGACAAGGGGACCGTCCACGTATTCAGTCTTAAATTCAATTCTGGGTCTCTTCAGAATGATATGTATCAGAATGATTCTTCTCTTTCCGTCTCACCATCTGGCTCCTCATTTTCGTTCATCAAAGGTAATGGAACCATTTGTGTCTAACCACGGATTTCCACTGTATTTGGATATTACTATTTCATAAATTCTTTGAATTGCTTTGAAATCACTTAGACTGGAATAAAATACAAAATCTGGTATAACAGCCAAGGAGTCCTTTGAAAATTTACTGGCATACTCCTGAAAAGAACACTTAGACTGGAATAAAATACAAAATCTGGTGTTGGGCAGCCACTGTCTCCTTATTTTAGTTGTATATTCCATTTTTCTTTCCATCTAAGAAGTTCGAATGCTTTAGTTTGGCTAGCGTGTCCATCGTGTTGAACACTTGGACATCTTGACACTTGAGGACATGTATCGAACATTCTTAGTATAGCAGATTTGTTGGACATTAGTTGTACAAAGTTAATTTAGGGCCCACATGTATTAAGTAGAATAAACTTCGAGAGAGAAATACATCAAAACTCAAATTTTAAGCATATAAATTTATGAACATATAGATTTTGAATTTCTTTCTTTTGCATAAAATTTTTATATATTTTAATGATGTCTGTCCTTGTGTGTGTCCTAAATTTGAACAATGTCTCAGTGTCTCGCGTCTATAACCATGCTTCTTACCTTCGAACAATACTAAACGAAAGGAAAGAGATGGGATATTCTCCTTATTTTTTTGCAGTCGGATATCCCTTTTTGCCTTTGGATCTAATGAACAGTCTTGCACCTGCAGGTGTGCTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGATTGCATGAAGGGTCTCGGTATGTTGTTGCGTTTGGTCACCAGAAGAATACAGTGGTGATCCTTGGAATGGATGGAAGGTAAATAGTCTTTGTAGTTCTTCTCTTACTATTGGGATTTTTTCATTTCTTTTTCAATTAATTATAATTCTGCAGAATAACAATTAAGATGAGGGTTGCTTGGGTATCCCAACTACGATTTCGATGATATTAGATAAATATTTTTAATGTGAACGCTTTATTTTCATAGACTGATAATAGACAATATTTTCTTTCTACTTTAACTTGAACCATTTGTTATTTGCTTCATTATCTTTTCTTTAGCATTGCCTAAGTCGTGTTAGCTTCCATTAGATCTGGTATTCTGTTTGGTCGTTTAACCGCTGCGTTGGAAGTTTTAGAATCTTGATGTGCCATTTTTAATTATTGCAGCTTCTACCGATGCCAATTTGATGCGGTGGATGGCGGAGAGATGACACAGCTTGAATATCACAACTTTCTAATGCCAGAGGAAGCCTTCTAGCCCAGTGTACTCTTTATTTCAGTTCTGTTGTAAGCATATTCTCTTCCTAGATCCTCTATTGCTTGTAAATAGATCTATGCATGTACAGATTGGCCTATGTTCGTCTCAGGGAAAAAAAAAAGGAAAAAGAAGAATTCTATTATTGCCCCCCATGTTCATATTTAATTTTCTTAACTTATTTGGCCTTATGAACAATTGTATACTCCAAACATGGGGAAAAGAAAACGTGTCTCCTGAAATTATTGCGAATGCAATAGGCTTTGATTGACTGTAATGCTAGCCTTCTGGATTTACCTGATGAAATTGAGACCTAAAATGAAGAGCTTACCATTAATGTACACATTCTTCTAAGAATAGCCTGCAGGTAGAAAAGTTGAACTAAATGATGGAGGACTTATTTATAGCGGAAGTTCAAGAAGT

mRNA sequence

TCTTATAAATCATCGCCATTATCTTCGTTTTCATCTGAATCTCGAGTTCCTCTGATTCTCGATGGAGACTCTCTCCGCCCTCCCTTCCTCTTCATGGCCGGAATCCAACTCCAATCCCAACACTCCGCCTGCTATTGCCGACTCCGAACGCTTTGATTCTGATTCCGTTTCGTCGTTGCCGCCTCAATCGCCGCAGACAGTGGAGACGGCTGGTTTGTCCACGGCGGCGGTTGATTCGCTCGCTTTGCAGCCTTCTCTTCTTCATCTCTCATTCAATCAAGACCATGGCTGCTTTGCTGCGGGCAGTGATCGAGGGTTTCGGATCTACAATTGCGATCCGTTCCGTGAGATTTTCAGAAGAGATTTTGAGCGTGGGGGTGGTATTGGCGTTGTGGAGATGCTTTTTCGGTGTAATATTTTGGCTCTTGTTGGTGGTGGACCTGAACCTCAGTACCCGCCTAATAAGGTCATGCTTTGGGATGATCATCAGAGCCGGTGTATTGGGGAGCTGTCGTTTCGGTCTGCTGTTCGGGGGGTTCGGCTACAGAGAGACCGAATTATTGTTATTTTGGAGCAGAAGGTTTTTGTGTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAATCCTAAAGGGCTTTGTGCTGTTTCTCAGCTGAGTTCTTCGCTTGTGCTTGTCTGCCCTGGATTGATTAAGGGGCAGGTTAGGGTTGAGCATTATGCCTCTCGACGGACAAAGTTTATCATGGCTCACGACTCGAGGGTTGCTTGCTTTGCGCTTACGACCAACGGGCAGTTGCTTGCTACAGCGAGCTCCAAGGGAACTTTGGTTCGGATATTCAATACAATTGATGGAAATTTGCTCCAAGAGGTACGGAGAGGCGCAGATAGGGCGGAGATATACAGTTTGGCCTTCTCATCGACTGCTCAATGGCTGGCAGTCTCCAGTGACAAGGGGACCGTCCACGTATTCAGTCTTAAATTCAATTCTGGGTCTCTTCAGAATGATATGTATCAGAATGATTCTTCTCTTTCCGTCTCACCATCTGGCTCCTCATTTTCGTTCATCAAAGGTGTGCTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGATTGCATGAAGGGTCTCGGTATGTTGTTGCGTTTGGTCACCAGAAGAATACAGTGGTGATCCTTGGAATGGATGGAAGCTTCTACCGATGCCAATTTGATGCGGTGGATGGCGGAGAGATGACACAGCTTGAATATCACAACTTTCTAATGCCAGAGGAAGCCTTCTAGCCCAGTGTACTCTTTATTTCAGTTCTGTTGTAAGCATATTCTCTTCCTAGATCCTCTATTGCTTGTAAATAGATCTATGCATGTACAGATTGGCCTATGTTCGTCTCAGGGAAAAAAAAAAGGAAAAAGAAGAATTCTATTATTGCCCCCCATGTTCATATTTAATTTTCTTAACTTATTTGGCCTTATGAACAATTGTATACTCCAAACATGGGGAAAAGAAAACGTGTCTCCTGAAATTATTGCGAATGCAATAGGCTTTGATTGACTGTAATGCTAGCCTTCTGGATTTACCTGATGAAATTGAGACCTAAAATGAAGAGCTTACCATTAATGTACACATTCTTCTAAGAATAGCCTGCAGGTAGAAAAGTTGAACTAAATGATGGAGGACTTATTTATAGCGGAAGTTCAAGAAGT

Coding sequence (CDS)

ATGGAGACTCTCTCCGCCCTCCCTTCCTCTTCATGGCCGGAATCCAACTCCAATCCCAACACTCCGCCTGCTATTGCCGACTCCGAACGCTTTGATTCTGATTCCGTTTCGTCGTTGCCGCCTCAATCGCCGCAGACAGTGGAGACGGCTGGTTTGTCCACGGCGGCGGTTGATTCGCTCGCTTTGCAGCCTTCTCTTCTTCATCTCTCATTCAATCAAGACCATGGCTGCTTTGCTGCGGGCAGTGATCGAGGGTTTCGGATCTACAATTGCGATCCGTTCCGTGAGATTTTCAGAAGAGATTTTGAGCGTGGGGGTGGTATTGGCGTTGTGGAGATGCTTTTTCGGTGTAATATTTTGGCTCTTGTTGGTGGTGGACCTGAACCTCAGTACCCGCCTAATAAGGTCATGCTTTGGGATGATCATCAGAGCCGGTGTATTGGGGAGCTGTCGTTTCGGTCTGCTGTTCGGGGGGTTCGGCTACAGAGAGACCGAATTATTGTTATTTTGGAGCAGAAGGTTTTTGTGTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAATCCTAAAGGGCTTTGTGCTGTTTCTCAGCTGAGTTCTTCGCTTGTGCTTGTCTGCCCTGGATTGATTAAGGGGCAGGTTAGGGTTGAGCATTATGCCTCTCGACGGACAAAGTTTATCATGGCTCACGACTCGAGGGTTGCTTGCTTTGCGCTTACGACCAACGGGCAGTTGCTTGCTACAGCGAGCTCCAAGGGAACTTTGGTTCGGATATTCAATACAATTGATGGAAATTTGCTCCAAGAGGTACGGAGAGGCGCAGATAGGGCGGAGATATACAGTTTGGCCTTCTCATCGACTGCTCAATGGCTGGCAGTCTCCAGTGACAAGGGGACCGTCCACGTATTCAGTCTTAAATTCAATTCTGGGTCTCTTCAGAATGATATGTATCAGAATGATTCTTCTCTTTCCGTCTCACCATCTGGCTCCTCATTTTCGTTCATCAAAGGTGTGCTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGATTGCATGAAGGGTCTCGGTATGTTGTTGCGTTTGGTCACCAGAAGAATACAGTGGTGATCCTTGGAATGGATGGAAGCTTCTACCGATGCCAATTTGATGCGGTGGATGGCGGAGAGATGACACAGCTTGAATATCACAACTTTCTAATGCCAGAGGAAGCCTTCTAG

Protein sequence

METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSLALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF
Homology
BLAST of CmoCh08G004020 vs. ExPASy Swiss-Prot
Match: Q93VB2 (Autophagy-related protein 18a OS=Arabidopsis thaliana OX=3702 GN=ATG18A PE=1 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 3.9e-168
Identity = 299/425 (70.35%), Postives = 336/425 (79.06%), Query Frame = 0

Query: 4   LSALPSSSWPESNSNPNTPPAIADSERFDS-----------DSVSSLPPQSPQTVETAGL 63
           ++ + SSSWP  N NP++  A      F S           DS SS+   S +  +T+  
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  STAAVDSLAL----QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGI 123
           S  +  +  L     PS+LHLSFNQDH CFA G+DRGFRI NCDPFREIFRRDF+RGGG+
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGP+PQYPPNKVM+WDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGL KGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSR+ACFALT +G LLATASSKGTLVRIFNT+DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LK NSGS   D  +     + S   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLM 414
            SEWSVAQFRL EG++Y+ AFGHQKNTVVILGMDGSFYRCQFD V+GGEM+QLEYHN L 
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQFDPVNGGEMSQLEYHNCLK 420

BLAST of CmoCh08G004020 vs. ExPASy Swiss-Prot
Match: Q0WPK3 (Autophagy-related protein 18d OS=Arabidopsis thaliana OX=3702 GN=ATG18D PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 5.5e-122
Identity = 211/355 (59.44%), Postives = 270/355 (76.06%), Query Frame = 0

Query: 63  QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILAL 122
           +  L+ +S+NQD+ CFAAG+  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILAL
Sbjct: 33  EAELVSVSWNQDYSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILAL 92

Query: 123 VGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 182
           VGGGP  QYP NKV++WDDHQ RCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL
Sbjct: 93  VGGGPNSQYPSNKVLIWDDHQGRCISEFTFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDL 152

Query: 183 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 242
           +LLHQIE +ANP+GLC +S   ++ VL CPG+ +G+VRVEH+     + I AHDS +AC 
Sbjct: 153 RLLHQIENMANPRGLCCLSHHMNTSVLACPGIRRGEVRVEHFGLNMVQIINAHDSNIACM 212

Query: 243 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 302
            LT +G LLATAS+KGTL+RIFNT+DG  LQEVRRG DRA+IYS+A S   QWLAVSSDK
Sbjct: 213 TLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDK 272

Query: 303 GTVHVFSLKF-----NSGSLQNDMYQNDSSLSVSPS-----GSSFSFIKGVLPKYFKSEW 362
           GTVH+FSL+      ++ S +++   N     VSP+     GSS SF++GVLPKYF SEW
Sbjct: 273 GTVHIFSLRVRVIGEDAYSTEHETSSNSLQPLVSPASGANPGSSLSFLRGVLPKYFSSEW 332

Query: 363 SVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFL 408
           S +QF + E ++Y  AFG Q NT+ I+G+DGSFYRC FD V+GGEMTQLE+ +FL
Sbjct: 333 SFSQFHVPEVTQYFAAFGAQ-NTIAIIGLDGSFYRCNFDPVNGGEMTQLEHFHFL 385

BLAST of CmoCh08G004020 vs. ExPASy Swiss-Prot
Match: Q8GYD7 (Autophagy-related protein 18c OS=Arabidopsis thaliana OX=3702 GN=ATG18C PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 3.6e-121
Identity = 214/361 (59.28%), Postives = 265/361 (73.41%), Query Frame = 0

Query: 63  QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILAL 122
           +  L+ + +NQD  CFAAG+  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILAL
Sbjct: 29  EAELVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILAL 88

Query: 123 VGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 182
           VGGGP  QYP +KV++WDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL
Sbjct: 89  VGGGPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDL 148

Query: 183 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 242
           +LLHQIET ANP+GLC +S  S++ VL CPGL +G++RVEH+     + I AHDS +AC 
Sbjct: 149 RLLHQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACM 208

Query: 243 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 302
            LT +G LLATAS+KGTL+RIFNT+DG  LQEVRRG DRA+IYS+A S   QWLAVSSDK
Sbjct: 209 TLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDK 268

Query: 303 GTVHVFSLKF-----------NSGSLQNDMYQNDSSLSVSPS-----GSSFSFIKGVLPK 362
           GTVH+FSL+            N   L    Y N     VSP+     GSS SF++GVLPK
Sbjct: 269 GTVHIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPK 328

Query: 363 YFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNF 408
           YF SEWS AQF + E +++  AFG   NTV I+GMDGSFYRC FD V+GGEM QLEY +F
Sbjct: 329 YFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHF 387

BLAST of CmoCh08G004020 vs. ExPASy Swiss-Prot
Match: Q9FHK8 (Autophagy-related protein 18e OS=Arabidopsis thaliana OX=3702 GN=ATG18E PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.1e-85
Identity = 165/350 (47.14%), Postives = 230/350 (65.71%), Query Frame = 0

Query: 66  LLHLSFNQDHGCFAAGSDRGFRIYNCDPF--REIFRRDFERGGGIGVVEMLFRCNILALV 125
           +L +++NQ    F  G++ GF +Y+C P   + I R   E   G  V EMLF  N+ A V
Sbjct: 33  VLSVAWNQVCSGFIVGTNHGFNVYSCKPMIKKSISRAPHE--SGFKVAEMLFLSNLFAFV 92

Query: 126 GGG-PEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 185
           G G    +YPPNKV +WDD+++ C+ EL+F+S V  V+L R+ ++V+L+Q ++VY F +L
Sbjct: 93  GNGYNNSEYPPNKVFVWDDYRNCCLSELTFKSEVIAVKLAREHVVVVLKQNIYVYTFNNL 152

Query: 186 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 245
           K+   IET+ NPKGLC V+ + S  VL CPG   GQV+V        KFI AHDS +AC 
Sbjct: 153 KVDRVIETLMNPKGLCCVTHVESKAVLACPGFHPGQVQVHDLRWNVIKFIKAHDSAIACM 212

Query: 246 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 305
            LT +G LLATAS+KGTL+RIFN +DG LLQE RRG +RAEIY++A SS  +W+A SS+K
Sbjct: 213 TLTLDGSLLATASTKGTLIRIFNAVDGTLLQEFRRGVERAEIYNVAISSNLKWVAASSEK 272

Query: 306 GTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKY-FKSEWSVAQFRLHE 365
           GT+HVF L+                LS  P+ SS SFI+ +LPKY +++E S AQF L  
Sbjct: 273 GTLHVFRLR-------------PDILSFDPASSS-SFIRVILPKYLYENERSFAQFSLPA 332

Query: 366 GSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEE 412
            ++++V FG  +NTV+++G+DGSF RC+FD  DGG+M +LE+  F   +E
Sbjct: 333 STKFIVGFG-SENTVLLVGIDGSFRRCKFDHADGGQMVELEHKYFFSLQE 365

BLAST of CmoCh08G004020 vs. ExPASy Swiss-Prot
Match: Q640T2 (WD repeat domain phosphoinositide-interacting protein 3 OS=Xenopus tropicalis OX=8364 GN=wdr45b PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.8e-77
Identity = 155/345 (44.93%), Postives = 217/345 (62.90%), Query Frame = 0

Query: 66  LLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILALVGG 125
           LL+  FNQDHGCFA G + GFR+YN DP +E  +++F   GG+G VEMLFRCN LALVGG
Sbjct: 13  LLYAGFNQDHGCFACGMENGFRVYNTDPLKEKEKQEFLE-GGVGYVEMLFRCNYLALVGG 72

Query: 126 GPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA-DLKL 185
           G +P+YPPNKVM+WDD + + + E+ F + V+ V+L+RDRI+V+L+  + V+ F  +   
Sbjct: 73  GKKPKYPPNKVMIWDDLKKKTVIEIEFSTEVKAVKLRRDRIVVVLDSMIKVFTFTHNPHQ 132

Query: 186 LHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYAS--RRTKFIMAHDSRVACF 245
           LH  ET  NPKGLC +   S++ +L  PG   G V++   AS  +    I AH+  ++C 
Sbjct: 133 LHVFETCYNPKGLCVLCPNSNNSLLAFPGAHTGHVQIVDLASTEKPPVDIPAHEGILSCI 192

Query: 246 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 305
           AL   G  +ATAS KGTL+RIF+T  G+L+QE+RRG+  A IY + F+  A  + VSSD 
Sbjct: 193 ALNLQGTRIATASEKGTLIRIFDTSSGHLIQELRRGSQAANIYCINFNEDASLICVSSDH 252

Query: 306 GTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 365
           GTVH+F+ +        D  +N  S   S S          LPKYF S+WS ++F++  G
Sbjct: 253 GTVHIFAAE--------DPKRNKQSSLASAS---------FLPKYFSSKWSFSKFQVPSG 312

Query: 366 SRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFL 408
           S  V AFG + N+V+ +  DGS+Y+ QF+    GE T+  Y  FL
Sbjct: 313 SPCVCAFGTEPNSVIAICADGSYYKFQFN--PKGECTRDVYAQFL 337

BLAST of CmoCh08G004020 vs. ExPASy TrEMBL
Match: A0A6J1HAV3 (autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111461124 PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.3e-235
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. ExPASy TrEMBL
Match: A0A6J1KWL2 (autophagy-related protein 18a-like OS=Cucurbita maxima OX=3661 GN=LOC111497824 PE=4 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 3.9e-232
Identity = 406/413 (98.31%), Postives = 410/413 (99.27%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWP+SNSN NTPPA+ADSERFDSDSVSSLPPQSPQ  ETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPDSNSNTNTPPAVADSERFDSDSVSSLPPQSPQKAETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGP+PQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPDPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAV+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVNGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. ExPASy TrEMBL
Match: A0A6J1JWT4 (autophagy-related protein 18a-like OS=Cucurbita maxima OX=3661 GN=LOC111488174 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.0e-211
Identity = 376/418 (89.95%), Postives = 395/418 (94.50%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSS----LPPQSPQTVETAGLSTAA 60
           METLSA PS  WP  N +PN PPA+ADSERFDSDSVSS    LPPQ PQTVET  +S+ A
Sbjct: 1   METLSAFPSPPWP--NPDPNPPPAVADSERFDSDSVSSEPPQLPPQLPQTVETT-VSSTA 60

Query: 61  VDSLALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFR 120
           V+  A QPSLLH+SFNQDHGCFAAG+DRGFRIYNCDPFREIFRRDF+RGGGIGVVEMLFR
Sbjct: 61  VEPPASQPSLLHISFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGIGVVEMLFR 120

Query: 121 CNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180
           CNILALVGGGP+PQYP NKVM+WDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV
Sbjct: 121 CNILALVGGGPDPQYPCNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180

Query: 181 YNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHD 240
           YNFADLKLLHQIETIANPKGLCAVSQLS+SLVLVCPGL KGQVRVEHYASRRTKFIMAHD
Sbjct: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240

Query: 241 SRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300
           SR+ACFALTTNGQLLATAS+KGTLVRIFNT+DGNLLQEVRRGADRAEIYSLAFSSTAQWL
Sbjct: 241 SRIACFALTTNGQLLATASTKGTLVRIFNTVDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300

Query: 301 AVSSDKGTVHVFSLKFNSGSLQNDM-YQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVA 360
           AVSSDKGTVHVFSLK NSGSL NDM +QNDSSLSV+PSGSSFSFIKGVLPKYFKSEWSVA
Sbjct: 301 AVSSDKGTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVA 360

Query: 361 QFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           QFRLHEGS+YVVAFGHQKNTVVILGMDGSFYRCQFD+V+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 415

BLAST of CmoCh08G004020 vs. ExPASy TrEMBL
Match: A0A6J1GPZ4 (autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111456412 PE=4 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 4.2e-210
Identity = 375/418 (89.71%), Postives = 393/418 (94.02%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLP----PQSPQTVETAGLSTAA 60
           METLSA PS  WP  N  PN PPA+A+SERFDSDSVSS P    PQ PQTVET  +S+ A
Sbjct: 1   METLSAFPSPPWP--NPEPNPPPAVAESERFDSDSVSSEPPQLLPQLPQTVETT-VSSTA 60

Query: 61  VDSLALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFR 120
           V+  A QPSLLHLSFNQDHGCFAAG+DRGFRIYNCDPFREIFRRDF+RGGGIGVVEMLFR
Sbjct: 61  VEPAASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGIGVVEMLFR 120

Query: 121 CNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180
           CNILALVGGGP+PQYP NKVM+WDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV
Sbjct: 121 CNILALVGGGPDPQYPCNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180

Query: 181 YNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHD 240
           YNFADLKLLHQIETIANPKGLCAVSQLS+SLVLVCPGL KGQVRVEHYASRRTKFIMAHD
Sbjct: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240

Query: 241 SRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300
           SR+ACFALTTNGQLLATAS+KGTLVRIFNT+DGNLLQEVRRGADRAEIYSLAFSSTAQWL
Sbjct: 241 SRIACFALTTNGQLLATASTKGTLVRIFNTVDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300

Query: 301 AVSSDKGTVHVFSLKFNSGSLQNDM-YQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVA 360
           AVSSDKGTVHVFSLK NSGSL NDM +QNDSSLSV+PSGSSFSFIKGVLPKYFKSEWSVA
Sbjct: 301 AVSSDKGTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVA 360

Query: 361 QFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           QFRLHEGS+YVVAFGHQKNTVVILGMDGSFYRCQFD+V+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 415

BLAST of CmoCh08G004020 vs. ExPASy TrEMBL
Match: A0A1S3BRA9 (autophagy-related protein 18a OS=Cucumis melo OX=3656 GN=LOC103492842 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.2e-205
Identity = 371/421 (88.12%), Postives = 386/421 (91.69%), Query Frame = 0

Query: 1   METLSALPSSSW----PESNSNPNTPPAIAD---SERFDSDSVSSLPPQSPQTVETAGLS 60
           METLSA PS  W    P  N NPN P A+AD   SERFDSDSVSS+ P         GLS
Sbjct: 1   METLSAFPSPPWPNPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP--------TGLS 60

Query: 61  TAAVDSLALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEM 120
             AV+  A QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDF+RGGG+GVVEM
Sbjct: 61  -PAVEPPASQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFDRGGGVGVVEM 120

Query: 121 LFRCNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQK 180
           LFRCNILALVGGGP+PQYPPNKVM+WDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQK
Sbjct: 121 LFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQK 180

Query: 181 VFVYNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIM 240
           VFVYNFADLKLLHQIETIANPKGLCAVSQLS+SLVLVCPGL KGQVRVEHYASRRTKFIM
Sbjct: 181 VFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIM 240

Query: 241 AHDSRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTA 300
           AHDSR+ACFALTTNGQLLATAS+KGTLVRIFNT DGNLLQEVRRGADRAEIYSLAFSSTA
Sbjct: 241 AHDSRIACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTA 300

Query: 301 QWLAVSSDKGTVHVFSLKFNSGSLQNDM-YQNDSSLSVSPSGSSFSFIKGVLPKYFKSEW 360
           QWLAVSSDKGTVHVFSLK NSGSL NDM +QNDSSLSV+PSGSSFSFIKGVLPKYFKSEW
Sbjct: 301 QWLAVSSDKGTVHVFSLKVNSGSLGNDMTHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEW 360

Query: 361 SVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEA 414
           SVAQFRLHEGS+YVVAFGHQKNTVVILGMDGSFYRCQFD+V+GGEMTQLEYHNFLMPEEA
Sbjct: 361 SVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEA 412

BLAST of CmoCh08G004020 vs. NCBI nr
Match: XP_022960384.1 (autophagy-related protein 18a-like [Cucurbita moschata])

HSP 1 Score: 825.1 bits (2130), Expect = 2.7e-235
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. NCBI nr
Match: KAG6593184.1 (Autophagy-related protein 18a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823.2 bits (2125), Expect = 1.0e-234
Identity = 412/413 (99.76%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAV+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVNGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. NCBI nr
Match: XP_023515100.1 (autophagy-related protein 18a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820.1 bits (2117), Expect = 8.7e-234
Identity = 409/413 (99.03%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWP+SNSNPNTPPA+ADSERFDSDSVSSLPPQSP+TVETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPDSNSNPNTPPAVADSERFDSDSVSSLPPQSPKTVETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAV+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVNGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. NCBI nr
Match: XP_023004559.1 (autophagy-related protein 18a-like [Cucurbita maxima])

HSP 1 Score: 813.5 bits (2100), Expect = 8.2e-232
Identity = 406/413 (98.31%), Postives = 410/413 (99.27%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWP+SNSN NTPPA+ADSERFDSDSVSSLPPQSPQ  ETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPDSNSNTNTPPAVADSERFDSDSVSSLPPQSPQKAETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGP+PQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPDPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYFKSEWSVAQFRLH 360

Query: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLMPEEAF 414
           EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAV+GGEMTQLEYHNFLMPEEAF
Sbjct: 361 EGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVNGGEMTQLEYHNFLMPEEAF 413

BLAST of CmoCh08G004020 vs. NCBI nr
Match: KAG7025574.1 (Autophagy-related protein 18a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 812.4 bits (2097), Expect = 1.8e-231
Identity = 412/430 (95.81%), Postives = 413/430 (96.05%), Query Frame = 0

Query: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60
           METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL
Sbjct: 1   METLSALPSSSWPESNSNPNTPPAIADSERFDSDSVSSLPPQSPQTVETAGLSTAAVDSL 60

Query: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120
           ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL
Sbjct: 61  ALQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNIL 120

Query: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180
           ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA
Sbjct: 121 ALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFA 180

Query: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240
           DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA
Sbjct: 181 DLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVA 240

Query: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300
           CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS
Sbjct: 241 CFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSS 300

Query: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIK-----------------GV 360
           DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIK                 GV
Sbjct: 301 DKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKVGYPFLPLDLMNSLAPAGV 360

Query: 361 LPKYFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEY 414
           LPKYFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAV+GGEMTQLEY
Sbjct: 361 LPKYFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVNGGEMTQLEY 420

BLAST of CmoCh08G004020 vs. TAIR 10
Match: AT3G62770.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 592.4 bits (1526), Expect = 2.8e-169
Identity = 299/425 (70.35%), Postives = 336/425 (79.06%), Query Frame = 0

Query: 4   LSALPSSSWPESNSNPNTPPAIADSERFDS-----------DSVSSLPPQSPQTVETAGL 63
           ++ + SSSWP  N NP++  A      F S           DS SS+   S +  +T+  
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  STAAVDSLAL----QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGI 123
           S  +  +  L     PS+LHLSFNQDH CFA G+DRGFRI NCDPFREIFRRDF+RGGG+
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGP+PQYPPNKVM+WDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGL KGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSR+ACFALT +G LLATASSKGTLVRIFNT+DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LK NSGS   D  +     + S   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFLM 414
            SEWSVAQFRL EG++Y+ AFGHQKNTVVILGMDGSFYRCQFD V+GGEM+QLEYHN L 
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQFDPVNGGEMSQLEYHNCLK 420

BLAST of CmoCh08G004020 vs. TAIR 10
Match: AT3G62770.3 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.1e-154
Identity = 277/395 (70.13%), Postives = 312/395 (78.99%), Query Frame = 0

Query: 4   LSALPSSSWPESNSNPNTPPAIADSERFDS-----------DSVSSLPPQSPQTVETAGL 63
           ++ + SSSWP  N NP++  A      F S           DS SS+   S +  +T+  
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  STAAVDSLAL----QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGI 123
           S  +  +  L     PS+LHLSFNQDH CFA G+DRGFRI NCDPFREIFRRDF+RGGG+
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGP+PQYPPNKVM+WDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGL KGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRVACFALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSR+ACFALT +G LLATASSKGTLVRIFNT+DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKFNSGSLQNDMYQNDSSLSVSPSGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LK NSGS   D  +     + S   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDG 384
            SEWSVAQFRL EG++Y+ AFGHQKNTVVILGMDG
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDG 395

BLAST of CmoCh08G004020 vs. TAIR 10
Match: AT3G56440.1 (homolog of yeast autophagy 18 (ATG18) D )

HSP 1 Score: 439.1 bits (1128), Expect = 3.9e-123
Identity = 211/355 (59.44%), Postives = 270/355 (76.06%), Query Frame = 0

Query: 63  QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILAL 122
           +  L+ +S+NQD+ CFAAG+  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILAL
Sbjct: 33  EAELVSVSWNQDYSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILAL 92

Query: 123 VGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 182
           VGGGP  QYP NKV++WDDHQ RCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL
Sbjct: 93  VGGGPNSQYPSNKVLIWDDHQGRCISEFTFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDL 152

Query: 183 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 242
           +LLHQIE +ANP+GLC +S   ++ VL CPG+ +G+VRVEH+     + I AHDS +AC 
Sbjct: 153 RLLHQIENMANPRGLCCLSHHMNTSVLACPGIRRGEVRVEHFGLNMVQIINAHDSNIACM 212

Query: 243 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 302
            LT +G LLATAS+KGTL+RIFNT+DG  LQEVRRG DRA+IYS+A S   QWLAVSSDK
Sbjct: 213 TLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDK 272

Query: 303 GTVHVFSLKF-----NSGSLQNDMYQNDSSLSVSPS-----GSSFSFIKGVLPKYFKSEW 362
           GTVH+FSL+      ++ S +++   N     VSP+     GSS SF++GVLPKYF SEW
Sbjct: 273 GTVHIFSLRVRVIGEDAYSTEHETSSNSLQPLVSPASGANPGSSLSFLRGVLPKYFSSEW 332

Query: 363 SVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNFL 408
           S +QF + E ++Y  AFG Q NT+ I+G+DGSFYRC FD V+GGEMTQLE+ +FL
Sbjct: 333 SFSQFHVPEVTQYFAAFGAQ-NTIAIIGLDGSFYRCNFDPVNGGEMTQLEHFHFL 385

BLAST of CmoCh08G004020 vs. TAIR 10
Match: AT2G40810.1 (homolog of yeast autophagy 18C )

HSP 1 Score: 436.4 bits (1121), Expect = 2.5e-122
Identity = 214/361 (59.28%), Postives = 265/361 (73.41%), Query Frame = 0

Query: 63  QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILAL 122
           +  L+ + +NQD  CFAAG+  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILAL
Sbjct: 29  EAELVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILAL 88

Query: 123 VGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 182
           VGGGP  QYP +KV++WDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL
Sbjct: 89  VGGGPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDL 148

Query: 183 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 242
           +LLHQIET ANP+GLC +S  S++ VL CPGL +G++RVEH+     + I AHDS +AC 
Sbjct: 149 RLLHQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACM 208

Query: 243 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 302
            LT +G LLATAS+KGTL+RIFNT+DG  LQEVRRG DRA+IYS+A S   QWLAVSSDK
Sbjct: 209 TLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDK 268

Query: 303 GTVHVFSLKF-----------NSGSLQNDMYQNDSSLSVSPS-----GSSFSFIKGVLPK 362
           GTVH+FSL+            N   L    Y N     VSP+     GSS SF++GVLPK
Sbjct: 269 GTVHIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPK 328

Query: 363 YFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNF 408
           YF SEWS AQF + E +++  AFG   NTV I+GMDGSFYRC FD V+GGEM QLEY +F
Sbjct: 329 YFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHF 387

BLAST of CmoCh08G004020 vs. TAIR 10
Match: AT2G40810.2 (homolog of yeast autophagy 18C )

HSP 1 Score: 436.4 bits (1121), Expect = 2.5e-122
Identity = 214/361 (59.28%), Postives = 265/361 (73.41%), Query Frame = 0

Query: 63  QPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFERGGGIGVVEMLFRCNILAL 122
           +  L+ + +NQD  CFAAG+  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILAL
Sbjct: 29  EAELVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILAL 88

Query: 123 VGGGPEPQYPPNKVMLWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 182
           VGGGP  QYP +KV++WDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL
Sbjct: 89  VGGGPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDL 148

Query: 183 KLLHQIETIANPKGLCAVSQLSSSLVLVCPGLIKGQVRVEHYASRRTKFIMAHDSRVACF 242
           +LLHQIET ANP+GLC +S  S++ VL CPGL +G++RVEH+     + I AHDS +AC 
Sbjct: 149 RLLHQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACM 208

Query: 243 ALTTNGQLLATASSKGTLVRIFNTIDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 302
            LT +G LLATAS+KGTL+RIFNT+DG  LQEVRRG DRA+IYS+A S   QWLAVSSDK
Sbjct: 209 TLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDK 268

Query: 303 GTVHVFSLKF-----------NSGSLQNDMYQNDSSLSVSPS-----GSSFSFIKGVLPK 362
           GTVH+FSL+            N   L    Y N     VSP+     GSS SF++GVLPK
Sbjct: 269 GTVHIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPK 328

Query: 363 YFKSEWSVAQFRLHEGSRYVVAFGHQKNTVVILGMDGSFYRCQFDAVDGGEMTQLEYHNF 408
           YF SEWS AQF + E +++  AFG   NTV I+GMDGSFYRC FD V+GGEM QLEY +F
Sbjct: 329 YFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHF 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VB23.9e-16870.35Autophagy-related protein 18a OS=Arabidopsis thaliana OX=3702 GN=ATG18A PE=1 SV=... [more]
Q0WPK35.5e-12259.44Autophagy-related protein 18d OS=Arabidopsis thaliana OX=3702 GN=ATG18D PE=2 SV=... [more]
Q8GYD73.6e-12159.28Autophagy-related protein 18c OS=Arabidopsis thaliana OX=3702 GN=ATG18C PE=2 SV=... [more]
Q9FHK83.1e-8547.14Autophagy-related protein 18e OS=Arabidopsis thaliana OX=3702 GN=ATG18E PE=2 SV=... [more]
Q640T21.8e-7744.93WD repeat domain phosphoinositide-interacting protein 3 OS=Xenopus tropicalis OX... [more]
Match NameE-valueIdentityDescription
A0A6J1HAV31.3e-235100.00autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111461124... [more]
A0A6J1KWL23.9e-23298.31autophagy-related protein 18a-like OS=Cucurbita maxima OX=3661 GN=LOC111497824 P... [more]
A0A6J1JWT45.0e-21189.95autophagy-related protein 18a-like OS=Cucurbita maxima OX=3661 GN=LOC111488174 P... [more]
A0A6J1GPZ44.2e-21089.71autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111456412... [more]
A0A1S3BRA93.2e-20588.12autophagy-related protein 18a OS=Cucumis melo OX=3656 GN=LOC103492842 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022960384.12.7e-235100.00autophagy-related protein 18a-like [Cucurbita moschata][more]
KAG6593184.11.0e-23499.76Autophagy-related protein 18a, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023515100.18.7e-23499.03autophagy-related protein 18a-like [Cucurbita pepo subsp. pepo][more]
XP_023004559.18.2e-23298.31autophagy-related protein 18a-like [Cucurbita maxima][more]
KAG7025574.11.8e-23195.81Autophagy-related protein 18a [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G62770.12.8e-16970.35Transducin/WD40 repeat-like superfamily protein [more]
AT3G62770.31.1e-15470.13Transducin/WD40 repeat-like superfamily protein [more]
AT3G56440.13.9e-12359.44homolog of yeast autophagy 18 (ATG18) D [more]
AT2G40810.12.5e-12259.28homolog of yeast autophagy 18C [more]
AT2G40810.22.5e-12259.28homolog of yeast autophagy 18C [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 225..265
e-value: 0.066
score: 22.3
coord: 53..90
e-value: 120.0
score: 3.8
coord: 268..309
e-value: 0.018
score: 24.2
IPR001680WD40 repeatPFAMPF00400WD40coord: 278..308
e-value: 0.0057
score: 17.5
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 35..386
e-value: 2.4E-19
score: 71.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..47
NoneNo IPR availablePANTHERPTHR11227:SF61BNAC06G18590D PROTEINcoord: 29..412
NoneNo IPR availablePANTHERPTHR11227WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATEDcoord: 29..412
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 65..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G004020.1CmoCh08G004020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
biological_process GO:0006497 protein lipidation
biological_process GO:0034497 protein localization to phagophore assembly site
cellular_component GO:0005829 cytosol
cellular_component GO:0019898 extrinsic component of membrane
cellular_component GO:0034045 phagophore assembly site membrane
molecular_function GO:0080025 phosphatidylinositol-3,5-bisphosphate binding
molecular_function GO:0032266 phosphatidylinositol-3-phosphate binding
molecular_function GO:0005515 protein binding