CmoCh08G003590 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G003590
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionElongation factor Tu
LocationCmo_Chr08: 2226167 .. 2230297 (-)
RNA-Seq ExpressionCmoCh08G003590
SyntenyCmoCh08G003590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCAAACCGGAAGAAGATTCTTCCCGCTTCTTTGTACTTTCTTCTTCTGGGACTACTTCCGGCGAGCGATTTGAGTTCCAATGGCCTCGGCTGCTCTCCGATGCTCTACCTCCAAGCGCCTTCTTGCCTATTCGTCCTCATTATCGTGGTGTTGCAGAGGATCCGCTTCCTCCCAATCTTCCATCTCCGAATTGCTTTCCAGAAACGATGGAACTTCCAGTCCCATTCCTTGGTGGAGATCCATGGCCACCTTCACTCGGACGTAAGCATTTTATACCTCTGAAGATTTTTCTCTCTTTTTTTTTTTTCAGCATGTATATGCGGTTGCCAGACGGATTTGTATTCAGTTGATGGTTTTGGTTTTTCTTGGTTCTCTGTAATTGGTTAGTCTTTTTTGTTAATATATTTCGATTTACATTGCGGGCGGACAGAAAGCCTCATGTGAATGTGGGGACAATTGGGCATGTTGACCATGGAAAGACTACATTGACTGCAGCTATTACGAAGGTTGGATTATCATTTTTTTTTTTTTTTTTTTTTTTAACGAAGTGCTAATGTAGCTTTGAGTCGTTGCTAGTAAAAACTTTATTATGTAGAAGGATTAATGTCGGATACTCAACACGTGGAGTTGTCGTTTGAATTTCAATTTCCTTTAGGTGTTGGCCGAGGAAGGGAAAGCTAAGGCTATAGCATTTGATGAAATTGATAAAGCTCCAGAGGAGAAAAAGAGAGGAATTACCATTGCAACGGTTGGTGTTCAAATTTTATTCTTGTTGGGAACTTACTTTCCATTTACGCTGTCGCCTATGCATATCTCTCCTCAAACCATCATTTGACTTGGGTTTATGATAAATTTCAATTTTGCAGGCTCACGTAGAGTATGAAACTGCTAAGAGACATTATGCACACGTAGATTGCCCCGGACATGCAGACTACGTTAAAGTAAGCCATGGATTGATATTTTTATTATTTAAAACAGAGCACAGTAATTTATTATTCAGTTATACGTTTGATTGGTGGGTTGATATAAATGTCATGGGCAAGGAGTTGTAAGTAGCAAGCATAAGTAGGTAGAATAATGAGGAATATTGGCGTTCTTCTTAACTAGAAGGTGGGTACTCAAGTCAGGTTCACTGCAAGCTGAATGCCATTTATTGGTGGACAACTTGCATATTGTACACTACTTGTATACAAGAGCAGGTTTCTTGACTTTTTTTTTTTACAGCTTTCAACATTTTAGTTTCCCCCAATGGCGCAATGTTTACATCAATTGGTTATAATTTTCTGATTATCGGAGATTTTATGAACAACTATTGAATGATGGTGGTAATTGGTGCAGAACATGATTACTGGAGCTGCTCAAATGGATGGTGGCATTCTTGTTGTGTCTGGTCCTGATGGGGCCATGCCTCAGACTAAGGAGCATATTCTACTAGCACGGCAGGTGTACATTTGTTTGTTTGGATCATATATGTTTTCTGCAGTGCAGATTTTCTTGTTTTTATGTGCTTTTGGGATGTAATGTTAATGCACTATAAATTTGTTTTATTGCATCATTCAGGTTGGTGTTCCATCACTTGTGTGCTTCTTGAATAAAGTTGACGCTGTTGATGATCCTGAATTGTTGGACCTTGTGGAAATGGAACTTCGTGGTATGATTACACAGTTGTATTTGGGTGCAGCTACTGTTTCCAGGATGTCAATTGTACATTTTAAATTGTGGTTTATGTTTTTGGCAGAACTTCTCAGCTTCTACAAGTTCCCTGGCGATGACATTCCCATCATTAGGGGTTCAGCTTTATCTGCATTACAGGGTACAAACGAAGAAATTGGTAAAAAGGCTATTCTACAGTTAATGGATGCTGTTGATGAATACATTCCCGACCCTGTTCGCCAGCTTGACAAGCCTTTCCTGATGCCAATTGAAGATGTTTTCTCTATTCAGGTATGGAGGACATGCCCTGGTCTTATATCGAAATAGTTCGTTCTGTTACCTTTTTTGTAATTATTTACCTGTGAACATGTTCATATAAAAAAAAAATACTATAAATTTAGACTGCTCATAATAGATTGTATATCATCAAGTTGGAACTTACATCCAGTTGAGTACTTGACATTTTTTGTGTAGACTGCACCATGTGCACCTATAGTTGCAAGTAAGTCGCAATGTGTAATGCTGATGTAATGAGTATAGGTTCACTTTTCAATTTCAGTACGTTGATGTTTGGCTGATAAAGTATGTATGAAGAGTTATTTTTGAAGTGAACTTGATTCTATCTGTTTATGCTGAAGGGTCGTGGAACTGTTGCTACTGGCCGTGTTGAGCAGGGAACTATTAAAGTTGGTGAAGAAGTTGAAGTTCTAGGGTTAGCTCAGGTAATTAGATATACCACATTCAATTTCTAACTATGTTTTGTGTGTGCGGGTATGGGCACACACCTTGTACCACTTTTGAGTCACTGTTGAACCTGCAACTAACTAGGGATGATTACAGTCTTGGGGCTAGGTATTGTGTGTTGTCATTGTGATCCTTGTTCGTGAAATTAAACATTGGTTTGATGATACTCTGTTCACGATGTCTTTTGCAGGGGCCTCCTTTGAAAACTACTGTAACTGGTGTTGAGATGTTCAAGAAAATCCTGGATCAAGGACAAGTAATTGGCTGTTAACTTTTACTTACCTCATTAATAATCACACACAAAAGAACAATGACAGAAGTAAAGTCCTAGAAAAAAGGAGCCAGAAAATTGGAAGCTACCTAAATACAAATAATTAAGTTGGTTTATGATTCTAACTTCGCTAGCACGTATTGTGTGTTTTTATTATACGAGGAAGAAAATTTAACACTAATACTGAAAGGACCTCCAATAGAACAAGCCAAATCAGTTGAAAAATTCCTTACACTTATCTAATGTTGACTTCATGTGTTTGTTAGGCTGGTGATAACGTCGGTCTCCTTCTCCGTGGTCTAAAAAGAGAAGACATTCAACGTGGACAGGTACGTACATTGGCATTACTCTTGCTGAAATGTTTTTGCATCTCATGGAAATTATCAATATAGATGACATTATGACAATGAGGTAGAAGGATTCATACGCTCAAGGAATTACATTTTGAAGGTGACTGTAGCCTACACACTTGGATATGATTGTTTCAGTGTTTTTTTTTTCTCTTTACTTATAGGTTATTGCAAAGCCTGGAACTCTCAAAACTTCCAAGAAATTTGAGGCCGAGATATATGTTCTCACAAAAGAGGAAGGTGGCCGACATACTGCTTTCATGTCCAACTACAGACCTCAATTTTACATGAGAACTGCAGATATCACTGGGAAGGTTGAATTACCTGAAAATGTTAAGATGGTTATGCCTGGTGACAATGTGACAGCAGCTTTTGAGTTGATTTTACCCGTTCCCCTTGAAGCAGGTGCGTCTCATTTAAACTCTCTTGGGATATAAAGACTAAATCTAAAGATTACTTCCTTTTCCCTTGCTTTGGTACTGCAAAATTTTGTTTATGTTTAGTGCATAAGTTGATTCAAATGTTCATAGAGAAGTTTCGGGTTTATTTTTTGAAAACCAATCAGCCTTGAATCCGATCACGTCCATGGATTAGGGTGCTTTTTTTCTTGCCAAAAATGTAATGCTGTATTATTTGCAGGACAAAGATTTGCCCTGAGGGAGGGTGGTAGAACGGTTGGTGCTGGAGTAGTCTCCAAAGTTCTTAGCTGAGCCTGAGGTACGGCGCCCATTTCCTTGAAAGCTCTGCCAAAACCAAACAATTTTTGAAGATCCATCCTTGACAACATGATGCTTTTCGACATTGTTTACAGAATCAGTTATCTATATTAGGCCCTTGAGAGATTATTAGGATGTTATGTAATTTGCATAGATTAAGGAAGCTGAATTTTATTGTCCTGAGTCCAGGATTCAAGATTATCTTGGTCCTCTTCATCTCTTCTTTCCATTTTCAAGTTGATCACTAATAATTTTAGAGTACATAAATGGCAATGGCATAATCAATGTACGTTCAATATTTAGTATTATGAACGTCAATTCCGATGGTTGTTTCTTATTGTTTTATGATAAACACTCTGGTGAGAAATACTATATATAAAATTTATTCTACTCGAG

mRNA sequence

GCCAAACCGGAAGAAGATTCTTCCCGCTTCTTTGTACTTTCTTCTTCTGGGACTACTTCCGGCGAGCGATTTGAGTTCCAATGGCCTCGGCTGCTCTCCGATGCTCTACCTCCAAGCGCCTTCTTGCCTATTCGTCCTCATTATCGTGGTGTTGCAGAGGATCCGCTTCCTCCCAATCTTCCATCTCCGAATTGCTTTCCAGAAACGATGGAACTTCCAGTCCCATTCCTTGGTGGAGATCCATGGCCACCTTCACTCGGACAAAGCCTCATGTGAATGTGGGGACAATTGGGCATGTTGACCATGGAAAGACTACATTGACTGCAGCTATTACGAAGGTGTTGGCCGAGGAAGGGAAAGCTAAGGCTATAGCATTTGATGAAATTGATAAAGCTCCAGAGGAGAAAAAGAGAGGAATTACCATTGCAACGGCTCACGTAGAGTATGAAACTGCTAAGAGACATTATGCACACGTAGATTGCCCCGGACATGCAGACTACGTTAAAAACATGATTACTGGAGCTGCTCAAATGGATGGTGGCATTCTTGTTGTGTCTGGTCCTGATGGGGCCATGCCTCAGACTAAGGAGCATATTCTACTAGCACGGCAGGTTGGTGTTCCATCACTTGTGTGCTTCTTGAATAAAGTTGACGCTGTTGATGATCCTGAATTGTTGGACCTTGTGGAAATGGAACTTCGTGAACTTCTCAGCTTCTACAAGTTCCCTGGCGATGACATTCCCATCATTAGGGGTTCAGCTTTATCTGCATTACAGGGTACAAACGAAGAAATTGGTAAAAAGGCTATTCTACAGTTAATGGATGCTGTTGATGAATACATTCCCGACCCTGTTCGCCAGCTTGACAAGCCTTTCCTGATGCCAATTGAAGATGTTTTCTCTATTCAGGGTCGTGGAACTGTTGCTACTGGCCGTGTTGAGCAGGGAACTATTAAAGTTGGTGAAGAAGTTGAAGTTCTAGGGTTAGCTCAGGGGCCTCCTTTGAAAACTACTGTAACTGGTGTTGAGATGTTCAAGAAAATCCTGGATCAAGGACAAGCTGGTGATAACGTCGGTCTCCTTCTCCGTGGTCTAAAAAGAGAAGACATTCAACGTGGACAGGTTATTGCAAAGCCTGGAACTCTCAAAACTTCCAAGAAATTTGAGGCCGAGATATATGTTCTCACAAAAGAGGAAGGTGGCCGACATACTGCTTTCATGTCCAACTACAGACCTCAATTTTACATGAGAACTGCAGATATCACTGGGAAGGTTGAATTACCTGAAAATGTTAAGATGGTTATGCCTGGTGACAATGTGACAGCAGCTTTTGAGTTGATTTTACCCGTTCCCCTTGAAGCAGGACAAAGATTTGCCCTGAGGGAGGGTGGTAGAACGGTTGGTGCTGGAGTAGTCTCCAAAGTTCTTAGCTGAGCCTGAGGTACGGCGCCCATTTCCTTGAAAGCTCTGCCAAAACCAAACAATTTTTGAAGATCCATCCTTGACAACATGATGCTTTTCGACATTGTTTACAGAATCAGTTATCTATATTAGGCCCTTGAGAGATTATTAGGATGTTATGTAATTTGCATAGATTAAGGAAGCTGAATTTTATTGTCCTGAGTCCAGGATTCAAGATTATCTTGGTCCTCTTCATCTCTTCTTTCCATTTTCAAGTTGATCACTAATAATTTTAGAGTACATAAATGGCAATGGCATAATCAATGTACGTTCAATATTTAGTATTATGAACGTCAATTCCGATGGTTGTTTCTTATTGTTTTATGATAAACACTCTGGTGAGAAATACTATATATAAAATTTATTCTACTCGAG

Coding sequence (CDS)

ATGGCCTCGGCTGCTCTCCGATGCTCTACCTCCAAGCGCCTTCTTGCCTATTCGTCCTCATTATCGTGGTGTTGCAGAGGATCCGCTTCCTCCCAATCTTCCATCTCCGAATTGCTTTCCAGAAACGATGGAACTTCCAGTCCCATTCCTTGGTGGAGATCCATGGCCACCTTCACTCGGACAAAGCCTCATGTGAATGTGGGGACAATTGGGCATGTTGACCATGGAAAGACTACATTGACTGCAGCTATTACGAAGGTGTTGGCCGAGGAAGGGAAAGCTAAGGCTATAGCATTTGATGAAATTGATAAAGCTCCAGAGGAGAAAAAGAGAGGAATTACCATTGCAACGGCTCACGTAGAGTATGAAACTGCTAAGAGACATTATGCACACGTAGATTGCCCCGGACATGCAGACTACGTTAAAAACATGATTACTGGAGCTGCTCAAATGGATGGTGGCATTCTTGTTGTGTCTGGTCCTGATGGGGCCATGCCTCAGACTAAGGAGCATATTCTACTAGCACGGCAGGTTGGTGTTCCATCACTTGTGTGCTTCTTGAATAAAGTTGACGCTGTTGATGATCCTGAATTGTTGGACCTTGTGGAAATGGAACTTCGTGAACTTCTCAGCTTCTACAAGTTCCCTGGCGATGACATTCCCATCATTAGGGGTTCAGCTTTATCTGCATTACAGGGTACAAACGAAGAAATTGGTAAAAAGGCTATTCTACAGTTAATGGATGCTGTTGATGAATACATTCCCGACCCTGTTCGCCAGCTTGACAAGCCTTTCCTGATGCCAATTGAAGATGTTTTCTCTATTCAGGGTCGTGGAACTGTTGCTACTGGCCGTGTTGAGCAGGGAACTATTAAAGTTGGTGAAGAAGTTGAAGTTCTAGGGTTAGCTCAGGGGCCTCCTTTGAAAACTACTGTAACTGGTGTTGAGATGTTCAAGAAAATCCTGGATCAAGGACAAGCTGGTGATAACGTCGGTCTCCTTCTCCGTGGTCTAAAAAGAGAAGACATTCAACGTGGACAGGTTATTGCAAAGCCTGGAACTCTCAAAACTTCCAAGAAATTTGAGGCCGAGATATATGTTCTCACAAAAGAGGAAGGTGGCCGACATACTGCTTTCATGTCCAACTACAGACCTCAATTTTACATGAGAACTGCAGATATCACTGGGAAGGTTGAATTACCTGAAAATGTTAAGATGGTTATGCCTGGTGACAATGTGACAGCAGCTTTTGAGTTGATTTTACCCGTTCCCCTTGAAGCAGGACAAAGATTTGCCCTGAGGGAGGGTGGTAGAACGGTTGGTGCTGGAGTAGTCTCCAAAGTTCTTAGCTGA

Protein sequence

MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELILPVPLEAGQRFALREGGRTVGAGVVSKVLS
Homology
BLAST of CmoCh08G003590 vs. ExPASy Swiss-Prot
Match: Q9ZT91 (Elongation factor Tu, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TUFA PE=1 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 2.2e-217
Identity = 390/454 (85.90%), Postives = 414/454 (91.19%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPI---PWWRSMAT 60
           MAS  LR  +SKRL+ +SS +   C  S +S  SIS  +  +D +SS      +WRSMAT
Sbjct: 1   MASVVLRNPSSKRLVPFSSQIYSRCGASVTSSYSISHSIGGDDLSSSTFGTSSFWRSMAT 60

Query: 61  FTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT 120
           FTR KPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT
Sbjct: 61  FTRNKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT 120

Query: 121 AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQ 180
           AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDG MPQTKEHILLARQ
Sbjct: 121 AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMPQTKEHILLARQ 180

Query: 181 VGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEE 240
           VGVPSLVCFLNKVD VDDPELL+LVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN+E
Sbjct: 181 VGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNDE 240

Query: 241 IGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEV 300
           IG++AIL+LMDAVDEYIPDPVR LDKPFLMPIEDVFSIQGRGTVATGR+EQG IKVGEEV
Sbjct: 241 IGRQAILKLMDAVDEYIPDPVRVLDKPFLMPIEDVFSIQGRGTVATGRIEQGVIKVGEEV 300

Query: 301 EVLGLAQ-GPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLK 360
           E+LGL + G PLK+TVTGVEMFKKILD GQAGDNVGLLLRGLKREDIQRG VIAKPG+ K
Sbjct: 301 EILGLREGGVPLKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVIAKPGSCK 360

Query: 361 TSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAA 420
           T KKFEAEIYVLTK+EGGRHTAF SNYRPQFY+RTADITGKVELPENVKMVMPGDNVTA 
Sbjct: 361 TYKKFEAEIYVLTKDEGGRHTAFFSNYRPQFYLRTADITGKVELPENVKMVMPGDNVTAV 420

Query: 421 FELILPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           FELI+PVPLE GQRFALREGGRTVGAGVVSKV++
Sbjct: 421 FELIMPVPLETGQRFALREGGRTVGAGVVSKVMT 454

BLAST of CmoCh08G003590 vs. ExPASy Swiss-Prot
Match: Q0BUQ2 (Elongation factor Tu OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) OX=391165 GN=tuf PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 5.0e-169
Identity = 291/393 (74.05%), Postives = 334/393 (84.99%), Query Frame = 0

Query: 56  ATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITI 115
           A F R KPH N+GTIGHVDHGKT+LTAAITKVLAE G A   A+D+IDKAPEE+ RGITI
Sbjct: 4   AKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEERARGITI 63

Query: 116 ATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLA 175
           +TAHVEYET  RHYAHVDCPGHADYVKNMITGAAQMDGGILVVS  DG MPQT+EHILLA
Sbjct: 64  STAHVEYETGNRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLA 123

Query: 176 RQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN 235
           RQVGVP+LV FLNKVD VDDPELLDLVEME+RELLS Y+FPGDDIPII+GSAL AL+  N
Sbjct: 124 RQVGVPALVVFLNKVDMVDDPELLDLVEMEVRELLSSYQFPGDDIPIIKGSALCALEDKN 183

Query: 236 EEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGE 295
            EIG+ AIL+LM+AVD YIP P R LD+PFLMPIEDVFSI GRGTV TGR+E+G +KVG+
Sbjct: 184 PEIGRDAILKLMEAVDAYIPQPERPLDRPFLMPIEDVFSISGRGTVVTGRIERGEVKVGD 243

Query: 296 EVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTL 355
           EVE++GL      KTTVTGVEMF+K+LD+G+AGDN+G LLRG KRED++RGQV+AKPG++
Sbjct: 244 EVEIVGLK--ATSKTTVTGVEMFRKLLDRGEAGDNIGALLRGTKREDVERGQVLAKPGSI 303

Query: 356 KTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTA 415
               KF AE Y+LTKEEGGRHT F +NYRPQFY RT D+TG V LPE  +MVMPGDNV+ 
Sbjct: 304 TPHTKFAAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVSLPEGTEMVMPGDNVSM 363

Query: 416 AFELILPVPLEAGQRFALREGGRTVGAGVVSKV 449
             ELI P+ ++ G RFA+REGGRTVGAGVV+K+
Sbjct: 364 QVELIAPIAMDEGLRFAIREGGRTVGAGVVAKI 394

BLAST of CmoCh08G003590 vs. ExPASy Swiss-Prot
Match: Q748X8 (Elongation factor Tu OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=tuf1 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.1e-167
Identity = 285/394 (72.34%), Postives = 334/394 (84.77%), Query Frame = 0

Query: 56  ATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITI 115
           A F RTKPHVN+GTIGHVDHGKTTLTAAITKVLAE G+A+   FD+ID APEE++RGITI
Sbjct: 4   AKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERGITI 63

Query: 116 ATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLA 175
           AT+HVEYET KRHYAHVDCPGHADYVKNMITGAAQMDG ILVVS  DG MPQT+EHILLA
Sbjct: 64  ATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLA 123

Query: 176 RQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN 235
           RQVGVP +V FLNK D VDD ELL+LVE+E+RELLS Y FPGDDIPII+GSAL  L G  
Sbjct: 124 RQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKGLNGDK 183

Query: 236 EEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGE 295
           +E+G++AIL+LM+AVD YIP+P R +DKPFLMP+EDVFSI GRGTVATGRVE+G +KVGE
Sbjct: 184 DELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVKVGE 243

Query: 296 EVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTL 355
           EVE++G+      KTTVTGVEMF+K+LD+G+AGDN+G LLRG+KREDI+RGQV+AKPG++
Sbjct: 244 EVEIVGIK--ATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSI 303

Query: 356 KTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTA 415
               KF+AE Y+LTKEEGGRHT F + YRPQFY RT D+TG V+LP   +MVMPGDNV  
Sbjct: 304 TPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVAV 363

Query: 416 AFELILPVPLEAGQRFALREGGRTVGAGVVSKVL 450
              LI P+ ++ G RFA+REGGRTVGAGVVS ++
Sbjct: 364 TINLITPIAMDEGLRFAIREGGRTVGAGVVSSII 395

BLAST of CmoCh08G003590 vs. ExPASy Swiss-Prot
Match: A5GAW4 (Elongation factor Tu OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=tuf1 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.1e-167
Identity = 287/394 (72.84%), Postives = 336/394 (85.28%), Query Frame = 0

Query: 56  ATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITI 115
           A F RTKPHVN+GTIGHVDHGKTTLTAAITKVLA +G+A+  AFD+ID APEE++RGITI
Sbjct: 4   AKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITI 63

Query: 116 ATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLA 175
           ATAHVEYET KRHYAHVDCPGHADYVKNMITGAAQMDG ILVVS  DG MPQT+EHILLA
Sbjct: 64  ATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLA 123

Query: 176 RQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN 235
           RQVGVP +V FLNK D VDD ELL+LVE+E+RELLS Y FPGDDIPII+GSAL AL+G  
Sbjct: 124 RQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKALEGDT 183

Query: 236 EEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGE 295
            E+G++AI++LM+AVD YIP+PVR +DKPFLMP+EDVFSI GRGTVATGRVE+G +KVGE
Sbjct: 184 GELGEQAIMKLMEAVDSYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVKVGE 243

Query: 296 EVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTL 355
           EVE++G+      KTTVTGVEMF+K+LD+G+AGDN+G LLRG+KREDI+RGQV+AKPG++
Sbjct: 244 EVEIVGMK--ATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSI 303

Query: 356 KTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTA 415
               KF+AE Y+LTKEEGGRHT F + YRPQFY RT D+TG VEL    +MVMPGDNV  
Sbjct: 304 TPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGIVELAAGTEMVMPGDNVAV 363

Query: 416 AFELILPVPLEAGQRFALREGGRTVGAGVVSKVL 450
              LI P+ ++ G RFA+REGGRTVGAGVVS ++
Sbjct: 364 TVNLITPIAMDEGLRFAIREGGRTVGAGVVSSII 395

BLAST of CmoCh08G003590 vs. ExPASy Swiss-Prot
Match: Q39Y08 (Elongation factor Tu OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=tuf1 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 4.7e-167
Identity = 284/394 (72.08%), Postives = 334/394 (84.77%), Query Frame = 0

Query: 56  ATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITI 115
           A F RTKPHVN+GTIGHVDHGKTTLTAAIT+VLAE G+A+   FD+ID APEE++RGITI
Sbjct: 4   AKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERGITI 63

Query: 116 ATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLA 175
           AT+HVEYET KRHYAHVDCPGHADYVKNMITGAAQMDG ILVVS  DG MPQT+EHILLA
Sbjct: 64  ATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLA 123

Query: 176 RQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN 235
           RQVGVP +V FLNK D VDD ELL+LVE+E+RELLS Y FPGDDIPII+GSAL AL G  
Sbjct: 124 RQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKALNGDK 183

Query: 236 EEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGE 295
           +E+G +AI++LMDAVD YIP+P R +DKPFLMP+EDVFSI GRGTVATGRVE+G +KVGE
Sbjct: 184 DELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVKVGE 243

Query: 296 EVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTL 355
           EVE++G+      KTTVTGVEMF+K+LD+G+AGDN+G LLRG+KREDI+RGQV+A+PG++
Sbjct: 244 EVEIVGIK--TTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGSI 303

Query: 356 KTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTA 415
               KF+AE Y+LTKEEGGRHT F + YRPQFY RT D+TG V+LP   +MVMPGDNV  
Sbjct: 304 TPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAV 363

Query: 416 AFELILPVPLEAGQRFALREGGRTVGAGVVSKVL 450
              LI P+ ++ G RFA+REGGRTVGAGVVS ++
Sbjct: 364 TVNLITPIAMDEGLRFAIREGGRTVGAGVVSSII 395

BLAST of CmoCh08G003590 vs. ExPASy TrEMBL
Match: A0A6J1H7F2 (Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111461175 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 9.8e-253
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR
Sbjct: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. ExPASy TrEMBL
Match: A0A6J1KUV3 (Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111497810 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.7e-249
Identity = 444/450 (98.67%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MASAALRC TSKRLLAYSSS SWCCRGSAS+QSSISELLSRNDGTSSPIPWWRSMATFTR
Sbjct: 1   MASAALRCYTSKRLLAYSSSSSWCCRGSASAQSSISELLSRNDGTSSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGT+KTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTIKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELP NVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPVNVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. ExPASy TrEMBL
Match: A0A6J1JXR8 (Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111488822 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.9e-244
Identity = 433/450 (96.22%), Postives = 442/450 (98.22%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MAS A R STSKRLLA+SS LSWCCRGSASS SSIS+LLSRNDG SSPIPWWRSMATFTR
Sbjct: 1   MASVAFRNSTSKRLLAHSSPLSWCCRGSASSHSSISDLLSRNDGASSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLA+EGKAKA+AFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLADEGKAKAVAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LM+AVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMEAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GL QGPP+KTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPG+LKT KK
Sbjct: 301 GLTQGPPMKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGSLKTYKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKV+S
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVIS 450

BLAST of CmoCh08G003590 vs. ExPASy TrEMBL
Match: A0A6J1GPX9 (Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111456042 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.4e-244
Identity = 433/450 (96.22%), Postives = 442/450 (98.22%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MAS ALR STSKRLLA+SS LSWCCRGSASS SSIS+LLSRNDG SSPIPWWRSMATFTR
Sbjct: 1   MASVALRNSTSKRLLAHSSPLSWCCRGSASSHSSISDLLSRNDGASSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKA+AFDEIDKAPEE+KRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAVAFDEIDKAPEERKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LM+AVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMEAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GL QG P+KTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPG+LKT KK
Sbjct: 301 GLTQGAPMKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGSLKTYKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKV+S
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVIS 450

BLAST of CmoCh08G003590 vs. ExPASy TrEMBL
Match: A0A6J1D7X3 (Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111018447 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 1.9e-243
Identity = 433/450 (96.22%), Postives = 441/450 (98.00%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MAS ALR +TSKRL A SS LSWCCRGSASS SSISEL+SR+D TSSP+PWWRSMATFTR
Sbjct: 1   MASVALRHTTSKRLHALSSPLSWCCRGSASSHSSISELISRDDATSSPVPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKA+AFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAVAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELL+FYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLNFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPG+LKTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGSLKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTA FELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTATFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
            PVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 SPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. NCBI nr
Match: XP_022960442.1 (elongation factor Tu, mitochondrial-like [Cucurbita moschata] >XP_023515142.1 elongation factor Tu, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6593136.1 Elongation factor Tu, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 882.1 bits (2278), Expect = 2.0e-252
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR
Sbjct: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. NCBI nr
Match: KAG7025536.1 (Elongation factor Tu, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 880.9 bits (2275), Expect = 4.5e-252
Identity = 449/450 (99.78%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELL+RNDGTSSPIPWWRSMATFTR
Sbjct: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLARNDGTSSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. NCBI nr
Match: XP_023004535.1 (elongation factor Tu, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 871.3 bits (2250), Expect = 3.6e-249
Identity = 444/450 (98.67%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MASAALRC TSKRLLAYSSS SWCCRGSAS+QSSISELLSRNDGTSSPIPWWRSMATFTR
Sbjct: 1   MASAALRCYTSKRLLAYSSSSSWCCRGSASAQSSISELLSRNDGTSSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGT+KTSKK
Sbjct: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTIKTSKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELP NVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPVNVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKVLS
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 450

BLAST of CmoCh08G003590 vs. NCBI nr
Match: XP_022992519.1 (elongation factor Tu, mitochondrial [Cucurbita maxima])

HSP 1 Score: 854.0 bits (2205), Expect = 5.9e-244
Identity = 433/450 (96.22%), Postives = 442/450 (98.22%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MAS A R STSKRLLA+SS LSWCCRGSASS SSIS+LLSRNDG SSPIPWWRSMATFTR
Sbjct: 1   MASVAFRNSTSKRLLAHSSPLSWCCRGSASSHSSISDLLSRNDGASSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLA+EGKAKA+AFDEIDKAPEEKKRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLADEGKAKAVAFDEIDKAPEEKKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LM+AVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMEAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GL QGPP+KTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPG+LKT KK
Sbjct: 301 GLTQGPPMKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGSLKTYKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKV+S
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVIS 450

BLAST of CmoCh08G003590 vs. NCBI nr
Match: XP_022953519.1 (elongation factor Tu, mitochondrial [Cucurbita moschata] >KAG6575887.1 Elongation factor Tu, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 433/450 (96.22%), Postives = 442/450 (98.22%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPIPWWRSMATFTR 60
           MAS ALR STSKRLLA+SS LSWCCRGSASS SSIS+LLSRNDG SSPIPWWRSMATFTR
Sbjct: 1   MASVALRNSTSKRLLAHSSPLSWCCRGSASSHSSISDLLSRNDGASSPIPWWRSMATFTR 60

Query: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHV 120
           TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKA+AFDEIDKAPEE+KRGITIATAHV
Sbjct: 61  TKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAVAFDEIDKAPEERKRGITIATAHV 120

Query: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180
           EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV
Sbjct: 121 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQVGV 180

Query: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240
           PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK
Sbjct: 181 PSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEEIGK 240

Query: 241 KAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300
           KAIL+LM+AVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL
Sbjct: 241 KAILKLMEAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEVEVL 300

Query: 301 GLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLKTSKK 360
           GL QG P+KTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPG+LKT KK
Sbjct: 301 GLTQGAPMKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGSLKTYKK 360

Query: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420
           FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI
Sbjct: 361 FEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAAFELI 420

Query: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           LPVPLEAGQRFALREGGRTVGAGVVSKV+S
Sbjct: 421 LPVPLEAGQRFALREGGRTVGAGVVSKVIS 450

BLAST of CmoCh08G003590 vs. TAIR 10
Match: AT4G02930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 756.1 bits (1951), Expect = 1.6e-218
Identity = 390/454 (85.90%), Postives = 414/454 (91.19%), Query Frame = 0

Query: 1   MASAALRCSTSKRLLAYSSSLSWCCRGSASSQSSISELLSRNDGTSSPI---PWWRSMAT 60
           MAS  LR  +SKRL+ +SS +   C  S +S  SIS  +  +D +SS      +WRSMAT
Sbjct: 1   MASVVLRNPSSKRLVPFSSQIYSRCGASVTSSYSISHSIGGDDLSSSTFGTSSFWRSMAT 60

Query: 61  FTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT 120
           FTR KPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT
Sbjct: 61  FTRNKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIAT 120

Query: 121 AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQTKEHILLARQ 180
           AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDG MPQTKEHILLARQ
Sbjct: 121 AHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMPQTKEHILLARQ 180

Query: 181 VGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNEE 240
           VGVPSLVCFLNKVD VDDPELL+LVEMELRELLSFYKFPGDDIPIIRGSALSALQGTN+E
Sbjct: 181 VGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKFPGDDIPIIRGSALSALQGTNDE 240

Query: 241 IGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGRGTVATGRVEQGTIKVGEEV 300
           IG++AIL+LMDAVDEYIPDPVR LDKPFLMPIEDVFSIQGRGTVATGR+EQG IKVGEEV
Sbjct: 241 IGRQAILKLMDAVDEYIPDPVRVLDKPFLMPIEDVFSIQGRGTVATGRIEQGVIKVGEEV 300

Query: 301 EVLGLAQ-GPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGLKREDIQRGQVIAKPGTLK 360
           E+LGL + G PLK+TVTGVEMFKKILD GQAGDNVGLLLRGLKREDIQRG VIAKPG+ K
Sbjct: 301 EILGLREGGVPLKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVIAKPGSCK 360

Query: 361 TSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKVELPENVKMVMPGDNVTAA 420
           T KKFEAEIYVLTK+EGGRHTAF SNYRPQFY+RTADITGKVELPENVKMVMPGDNVTA 
Sbjct: 361 TYKKFEAEIYVLTKDEGGRHTAFFSNYRPQFYLRTADITGKVELPENVKMVMPGDNVTAV 420

Query: 421 FELILPVPLEAGQRFALREGGRTVGAGVVSKVLS 451
           FELI+PVPLE GQRFALREGGRTVGAGVVSKV++
Sbjct: 421 FELIMPVPLETGQRFALREGGRTVGAGVVSKVMT 454

BLAST of CmoCh08G003590 vs. TAIR 10
Match: AT4G20360.1 (RAB GTPase homolog E1B )

HSP 1 Score: 520.4 bits (1339), Expect = 1.4e-147
Identity = 292/476 (61.34%), Postives = 342/476 (71.85%), Query Frame = 0

Query: 3   SAALRCSTSKRLL-AYSSSLSWCCRGSASSQS------SISELLSRNDGTSSPIPWWRSM 62
           SA   CS+S R+L +YSS     C   ++S        S S L S +  T+S     R  
Sbjct: 4   SAPAACSSSSRILCSYSSPSPSLCPAISTSGKLKTLTLSSSFLPSYSLTTTSASQSTRRS 63

Query: 63  AT-------FTRTKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEE 122
            T       F R KPHVN+GTIGHVDHGKTTLTAA+T  LA  G + A  +DEID APEE
Sbjct: 64  FTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDEIDAAPEE 123

Query: 123 KKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGAMPQT 182
           + RGITI TA VEYET  RHYAHVDCPGHADYVKNMITGAAQMDG ILVVSG DG MPQT
Sbjct: 124 RARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQT 183

Query: 183 KEHILLARQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPGDDIPIIRGSAL 242
           KEHILLA+QVGVP +V FLNK D VDD ELL+LVE+E+RELLS Y+F GDDIPII GSAL
Sbjct: 184 KEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSYEFNGDDIPIISGSAL 243

Query: 243 SALQ----------GTNEEIGKKAILQLMDAVDEYIPDPVRQLDKPFLMPIEDVFSIQGR 302
            A++          G N+ + K  I +LMDAVD+YIP P RQ + PFL+ +EDVFSI GR
Sbjct: 244 LAVETLTENPKVKRGDNKWVDK--IYELMDAVDDYIPIPQRQTELPFLLAVEDVFSITGR 303

Query: 303 GTVATGRVEQGTIKVGEEVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAGDNVGLLLRGL 362
           GTVATGRVE+GT+KVGE V+++GL +      TVTGVEMF+KILD+  AGDNVGLLLRG+
Sbjct: 304 GTVATGRVERGTVKVGETVDLVGLRE--TRSYTVTGVEMFQKILDEALAGDNVGLLLRGI 363

Query: 363 KREDIQRGQVIAKPGTLKTSKKFEAEIYVLTKEEGGRHTAFMSNYRPQFYMRTADITGKV 422
           ++ DIQRG V+AKPG++    KFEA IYVL KEEGGRH+ F + YRPQFYMRT D+TGKV
Sbjct: 364 QKADIQRGMVLAKPGSITPHTKFEAIIYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKV 423

Query: 423 -----ELPENVKMVMPGDNVTAAFELILPVPLEAGQRFALREGGRTVGAGVVSKVL 450
                +  E  KMVMPGD V    ELI+PV  E G RFA+REGG+TVGAGV+  +L
Sbjct: 424 TKIMNDKDEESKMVMPGDRVKIVVELIVPVACEQGMRFAIREGGKTVGAGVIGTIL 475

BLAST of CmoCh08G003590 vs. TAIR 10
Match: AT1G07920.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-42
Identity = 132/436 (30.28%), Postives = 210/436 (48.17%), Query Frame = 0

Query: 60  RTKPHVNVGTIGHVDHGKTTLT-------AAITKVLAEEGKAKAIAFDE--------IDK 119
           + K H+N+  IGHVD GK+T T         I K + E  + +A   ++        +DK
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 120 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGA 179
              E++RGITI  A  ++ET K +   +D PGH D++KNMITG +Q D  +L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 180 MP-------QTKEHILLARQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPG 239
                    QT+EH LLA  +GV  ++C  NK+DA          +  ++E+ S+ K  G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 240 ---DDIPIIRGSALSALQGTNEEIGKKAILQ------LMDAVDEYIPDPVRQLDKPFLMP 299
              D IP +    +S  +G N  I +   L       L++A+D+ I +P R  DKP  +P
Sbjct: 183 YNPDKIPFV---PISGFEGDN-MIERSTNLDWYKGPTLLEALDQ-INEPKRPSDKPLRLP 242

Query: 300 IEDVFSIQGRGTVATGRVEQGTIKVGEEVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAG 359
           ++DV+ I G GTV  GRVE G IK G  V          L T V  VEM  + L +   G
Sbjct: 243 LQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTG----LTTEVKSVEMHHESLLEALPG 302

Query: 360 DNVGLLLRGLKREDIQRGQVI--AKPGTLKTSKKFEAEIYVLTK--EEGGRHTAFMSNYR 419
           DNVG  ++ +  +D++RG V   +K    K +  F +++ ++    + G  +   +  + 
Sbjct: 303 DNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHT 362

Query: 420 PQFYMRTADITGKV------ELPENVKMVMPGDNVTAAFELILPVPLEAGQ------RFA 449
               ++ ++I  K+      E+ +  K +  GD          P+ +E         RFA
Sbjct: 363 SHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFA 422

BLAST of CmoCh08G003590 vs. TAIR 10
Match: AT1G07930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-42
Identity = 132/436 (30.28%), Postives = 210/436 (48.17%), Query Frame = 0

Query: 60  RTKPHVNVGTIGHVDHGKTTLT-------AAITKVLAEEGKAKAIAFDE--------IDK 119
           + K H+N+  IGHVD GK+T T         I K + E  + +A   ++        +DK
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 120 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGA 179
              E++RGITI  A  ++ET K +   +D PGH D++KNMITG +Q D  +L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 180 MP-------QTKEHILLARQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPG 239
                    QT+EH LLA  +GV  ++C  NK+DA          +  ++E+ S+ K  G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 240 ---DDIPIIRGSALSALQGTNEEIGKKAILQ------LMDAVDEYIPDPVRQLDKPFLMP 299
              D IP +    +S  +G N  I +   L       L++A+D+ I +P R  DKP  +P
Sbjct: 183 YNPDKIPFV---PISGFEGDN-MIERSTNLDWYKGPTLLEALDQ-INEPKRPSDKPLRLP 242

Query: 300 IEDVFSIQGRGTVATGRVEQGTIKVGEEVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAG 359
           ++DV+ I G GTV  GRVE G IK G  V          L T V  VEM  + L +   G
Sbjct: 243 LQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTG----LTTEVKSVEMHHESLLEALPG 302

Query: 360 DNVGLLLRGLKREDIQRGQVI--AKPGTLKTSKKFEAEIYVLTK--EEGGRHTAFMSNYR 419
           DNVG  ++ +  +D++RG V   +K    K +  F +++ ++    + G  +   +  + 
Sbjct: 303 DNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHT 362

Query: 420 PQFYMRTADITGKV------ELPENVKMVMPGDNVTAAFELILPVPLEAGQ------RFA 449
               ++ ++I  K+      E+ +  K +  GD          P+ +E         RFA
Sbjct: 363 SHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFA 422

BLAST of CmoCh08G003590 vs. TAIR 10
Match: AT1G07940.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-42
Identity = 132/436 (30.28%), Postives = 210/436 (48.17%), Query Frame = 0

Query: 60  RTKPHVNVGTIGHVDHGKTTLT-------AAITKVLAEEGKAKAIAFDE--------IDK 119
           + K H+N+  IGHVD GK+T T         I K + E  + +A   ++        +DK
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 120 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGA 179
              E++RGITI  A  ++ET K +   +D PGH D++KNMITG +Q D  +L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 180 MP-------QTKEHILLARQVGVPSLVCFLNKVDAVDDPELLDLVEMELRELLSFYKFPG 239
                    QT+EH LLA  +GV  ++C  NK+DA          +  ++E+ S+ K  G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 240 ---DDIPIIRGSALSALQGTNEEIGKKAILQ------LMDAVDEYIPDPVRQLDKPFLMP 299
              D IP +    +S  +G N  I +   L       L++A+D+ I +P R  DKP  +P
Sbjct: 183 YNPDKIPFV---PISGFEGDN-MIERSTNLDWYKGPTLLEALDQ-INEPKRPSDKPLRLP 242

Query: 300 IEDVFSIQGRGTVATGRVEQGTIKVGEEVEVLGLAQGPPLKTTVTGVEMFKKILDQGQAG 359
           ++DV+ I G GTV  GRVE G IK G  V          L T V  VEM  + L +   G
Sbjct: 243 LQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTG----LTTEVKSVEMHHESLLEALPG 302

Query: 360 DNVGLLLRGLKREDIQRGQVI--AKPGTLKTSKKFEAEIYVLTK--EEGGRHTAFMSNYR 419
           DNVG  ++ +  +D++RG V   +K    K +  F +++ ++    + G  +   +  + 
Sbjct: 303 DNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHT 362

Query: 420 PQFYMRTADITGKV------ELPENVKMVMPGDNVTAAFELILPVPLEAGQ------RFA 449
               ++ ++I  K+      E+ +  K +  GD          P+ +E         RFA
Sbjct: 363 SHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFA 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZT912.2e-21785.90Elongation factor Tu, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TUFA PE=1... [more]
Q0BUQ25.0e-16974.05Elongation factor Tu OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNI... [more]
Q748X82.1e-16772.34Elongation factor Tu OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 ... [more]
A5GAW42.1e-16772.84Elongation factor Tu OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=tuf1 ... [more]
Q39Y084.7e-16772.08Elongation factor Tu OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H7F29.8e-253100.00Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111461175 PE=3 SV=1[more]
A0A6J1KUV31.7e-24998.67Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111497810 PE=3 SV=1[more]
A0A6J1JXR82.9e-24496.22Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111488822 PE=3 SV=1[more]
A0A6J1GPX98.4e-24496.22Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111456042 PE=3 SV=1[more]
A0A6J1D7X31.9e-24396.22Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111018447 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022960442.12.0e-252100.00elongation factor Tu, mitochondrial-like [Cucurbita moschata] >XP_023515142.1 el... [more]
KAG7025536.14.5e-25299.78Elongation factor Tu, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023004535.13.6e-24998.67elongation factor Tu, mitochondrial-like [Cucurbita maxima][more]
XP_022992519.15.9e-24496.22elongation factor Tu, mitochondrial [Cucurbita maxima][more]
XP_022953519.11.7e-24396.22elongation factor Tu, mitochondrial [Cucurbita moschata] >KAG6575887.1 Elongatio... [more]
Match NameE-valueIdentityDescription
AT4G02930.11.6e-21885.90GTP binding Elongation factor Tu family protein [more]
AT4G20360.11.4e-14761.34RAB GTPase homolog E1B [more]
AT1G07920.14.8e-4230.28GTP binding Elongation factor Tu family protein [more]
AT1G07930.14.8e-4230.28GTP binding Elongation factor Tu family protein [more]
AT1G07940.14.8e-4230.28GTP binding Elongation factor Tu family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000795Translational (tr)-type GTP-binding domainPRINTSPR00315ELONGATNFCTcoord: 130..140
score: 63.79
coord: 183..192
score: 47.21
coord: 110..118
score: 65.52
coord: 66..79
score: 73.94
coord: 146..157
score: 58.26
IPR000795Translational (tr)-type GTP-binding domainPFAMPF00009GTP_EFTUcoord: 62..255
e-value: 2.0E-54
score: 184.1
IPR000795Translational (tr)-type GTP-binding domainPROSITEPS51722G_TR_2coord: 62..258
score: 58.068672
IPR004160Translation elongation factor EFTu/EF1A, C-terminalPFAMPF03143GTP_EFTU_D3coord: 355..448
e-value: 1.6E-35
score: 121.6
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 65..197
e-value: 9.1E-13
score: 46.3
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 394..449
e-value: 8.9E-26
score: 91.5
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 260..390
e-value: 7.3E-56
score: 189.6
NoneNo IPR availablePANTHERPTHR43721ELONGATION FACTOR TU-RELATEDcoord: 11..449
NoneNo IPR availablePANTHERPTHR43721:SF23ELONGATION FACTOR TUcoord: 11..449
NoneNo IPR availableCDDcd03707EFTU_IIIcoord: 356..445
e-value: 6.59672E-56
score: 178.474
IPR004161Translation elongation factor EFTu-like, domain 2PFAMPF03144GTP_EFTU_D2coord: 279..349
e-value: 1.4E-17
score: 63.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 54..257
e-value: 2.7E-72
score: 244.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 60..280
IPR004541Translation elongation factor EFTu/EF1A, bacterial/organelleTIGRFAMTIGR00485TIGR00485coord: 54..449
e-value: 7.0E-221
score: 730.8
IPR004541Translation elongation factor EFTu/EF1A, bacterial/organelleHAMAPMF_00118_BEF_Tu_Bcoord: 53..450
score: 57.981899
IPR031157Tr-type G domain, conserved sitePROSITEPS00301G_TR_1coord: 103..118
IPR033720Elongation factor Tu, domain 2CDDcd03697EFTU_IIcoord: 265..353
e-value: 1.14803E-51
score: 167.31
IPR041709Elongation factor Tu (EF-Tu), GTP-binding domainCDDcd01884EF_Tucoord: 63..257
e-value: 1.53381E-149
score: 421.222
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 259..355
IPR009001Translation elongation factor EF1A/initiation factor IF2gamma, C-terminalSUPERFAMILY50465EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domaincoord: 359..449

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G003590.1CmoCh08G003590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070125 mitochondrial translational elongation
biological_process GO:0046686 response to cadmium ion
biological_process GO:0006414 translational elongation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0003746 translation elongation factor activity