CmoCh08G003530 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G003530
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	basic 7S globulin 2-like
Location	Cmo_Chr08: 2183841 .. 2185193 (-)
RNA-Seq Expression	CmoCh08G003530
Synteny	CmoCh08G003530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGCCTCTGTTCTTCGTGTTCTTCACTTTCTCTCTCCTTTCCTTTCCATTTCTCTCTCTCCAAACAGCCTTTATCGCTCCCATTCACAAAGATCACAACTCCGGCCTCTACTCCATCTCCGTCCACCTCAAAACTCCCCTCCGTCCGGCCAATCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCACTACAACTCCTCCTCTTACCGCCTCGTCAAATGCAATTCTCCTCTCTCCGATTCTTTCAACCACGGCGTTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCTAACGACACCATTTTCTCCTACGTCTACCCCGAGAAACCCTCGATTCGAGACGAATACGTCGATTACCTTAACGCGAAGCTTACAGATTCCGAGAATGTAGTCACCGATGTTCTTGCTCTGTCCACCACCGACGGCTCCCGTTCCGGCTCACTCCGTCGAATTTCTAACATGCCGTTTGCCTGTGTGAAGACAAATTTCCTCCGAGGACTTGCTCGGAATGTAATTGGCCTGGCGGCGCTCGGCCGTGCGAACGAATCGATTCCGTTGACGATTAGTGCGAAATTCAATAGCCCTAGAATCTTCGCGATTTGTTTATCGGGAACGAGATTAGGATCTGGTGTAGCGTTTATCGGATCTAAAGGACCGTACGCATTTTCCCCCAATGTTGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCAAAGCGGCTCAATCTACACGAATTGGTTGCCATCTTACGAATATTACATTGGACTCTCCGCCATCAGAATCAACAACGAGGCGGTGCGATTCAACACATCCTTGTTGCAGTTTGAGCCGGTTCACGGCCGCGGCGGGGCGAAGATCAGCACCTCCACTACCTACGCTCTGCTCCAGAGCTCTATTTACAGAGCATTCGCGATGGCGTTTATGAAGGAAGCGGCTATGCTGAACTTCACGTTGGCGAATGCGGTGGAGCCGTTCGGCGTGTGCTTTGAGGGGAGCAGCGTTGAGATGACGGCGGCGGGACCGAGGGCGCCGGTGGTGTATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATTCGATGGTGAGGATTAAGAAGCTGGGAGTGGATTTGTGGTGCTTAGGATATGTTAATGGCGGGGAGTTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAAGTACAGATTTGGGTTTAGCTCGTCGGCGTTGCTGCAAGGGACTTCGTGTTCTAAGTTCAACTTCAGTTCTTCCGACAATTTCCTATAA

mRNA sequence

ATGGCGACGCCTCTGTTCTTCGTGTTCTTCACTTTCTCTCTCCTTTCCTTTCCATTTCTCTCTCTCCAAACAGCCTTTATCGCTCCCATTCACAAAGATCACAACTCCGGCCTCTACTCCATCTCCGTCCACCTCAAAACTCCCCTCCGTCCGGCCAATCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCACTACAACTCCTCCTCTTACCGCCTCGTCAAATGCAATTCTCCTCTCTCCGATTCTTTCAACCACGGCGTTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCTAACGACACCATTTTCTCCTACGTCTACCCCGAGAAACCCTCGATTCGAGACGAATACGTCGATTACCTTAACGCGAAGCTTACAGATTCCGAGAATGTAGTCACCGATGTTCTTGCTCTGTCCACCACCGACGGCTCCCGTTCCGGCTCACTCCGTCGAATTTCTAACATGCCGTTTGCCTGTGTGAAGACAAATTTCCTCCGAGGACTTGCTCGGAATGTAATTGGCCTGGCGGCGCTCGGCCGTGCGAACGAATCGATTCCGTTGACGATTAGTGCGAAATTCAATAGCCCTAGAATCTTCGCGATTTGTTTATCGGGAACGAGATTAGGATCTGGTGTAGCGTTTATCGGATCTAAAGGACCGTACGCATTTTCCCCCAATGTTGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCAAAGCGGCTCAATCTACACGAATTGGTTGCCATCTTACGAATATTACATTGGACTCTCCGCCATCAGAATCAACAACGAGGCGGTGCGATTCAACACATCCTTGTTGCAGTTTGAGCCGGTTCACGGCCGCGGCGGGGCGAAGATCAGCACCTCCACTACCTACGCTCTGCTCCAGAGCTCTATTTACAGAGCATTCGCGATGGCGTTTATGAAGGAAGCGGCTATGCTGAACTTCACGTTGGCGAATGCGGTGGAGCCGTTCGGCGTGTGCTTTGAGGGGAGCAGCGTTGAGATGACGGCGGCGGGACCGAGGGCGCCGGTGGTGTATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATTCGATGGTGAGGATTAAGAAGCTGGGAGTGGATTTGTGGTGCTTAGGATATGTTAATGGCGGGGAGTTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAAGTACAGATTTGGGTTTAGCTCGTCGGCGTTGCTGCAAGGGACTTCGTGTTCTAAGTTCAACTTCAGTTCTTCCGACAATTTCCTATAA

Coding sequence (CDS)

ATGGCGACGCCTCTGTTCTTCGTGTTCTTCACTTTCTCTCTCCTTTCCTTTCCATTTCTCTCTCTCCAAACAGCCTTTATCGCTCCCATTCACAAAGATCACAACTCCGGCCTCTACTCCATCTCCGTCCACCTCAAAACTCCCCTCCGTCCGGCCAATCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCACTACAACTCCTCCTCTTACCGCCTCGTCAAATGCAATTCTCCTCTCTCCGATTCTTTCAACCACGGCGTTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCTAACGACACCATTTTCTCCTACGTCTACCCCGAGAAACCCTCGATTCGAGACGAATACGTCGATTACCTTAACGCGAAGCTTACAGATTCCGAGAATGTAGTCACCGATGTTCTTGCTCTGTCCACCACCGACGGCTCCCGTTCCGGCTCACTCCGTCGAATTTCTAACATGCCGTTTGCCTGTGTGAAGACAAATTTCCTCCGAGGACTTGCTCGGAATGTAATTGGCCTGGCGGCGCTCGGCCGTGCGAACGAATCGATTCCGTTGACGATTAGTGCGAAATTCAATAGCCCTAGAATCTTCGCGATTTGTTTATCGGGAACGAGATTAGGATCTGGTGTAGCGTTTATCGGATCTAAAGGACCGTACGCATTTTCCCCCAATGTTGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCAAAGCGGCTCAATCTACACGAATTGGTTGCCATCTTACGAATATTACATTGGACTCTCCGCCATCAGAATCAACAACGAGGCGGTGCGATTCAACACATCCTTGTTGCAGTTTGAGCCGGTTCACGGCCGCGGCGGGGCGAAGATCAGCACCTCCACTACCTACGCTCTGCTCCAGAGCTCTATTTACAGAGCATTCGCGATGGCGTTTATGAAGGAAGCGGCTATGCTGAACTTCACGTTGGCGAATGCGGTGGAGCCGTTCGGCGTGTGCTTTGAGGGGAGCAGCGTTGAGATGACGGCGGCGGGACCGAGGGCGCCGGTGGTGTATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATTCGATGGTGAGGATTAAGAAGCTGGGAGTGGATTTGTGGTGCTTAGGATATGTTAATGGCGGGGAGTTTCCGAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAAGTACAGATTTGGGTTTAGCTCGTCGGCGTTGCTGCAAGGGACTTCGTGTTCTAAGTTCAACTTCAGTTCTTCCGACAATTTCCTATAA

Protein sequence

MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSIRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFGFSSSALLQGTSCSKFNFSSSDNFL

Homology

[Close]

BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 8.7e-89
Identity = 188/443 (42.44%), Postives = 262/443 (59.14%), Query Frame = 0


Query: 10  FTFSLLSFPFL--SLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWIDC 69
           F  ++L F FL  + Q   IAPI KD  + LY++SV LKTPL+P  LHL LG + SW+ C
Sbjct: 11  FNLAIL-FLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLC 70

Query: 70  YNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSIRDEYVD 129
            + Y SSS   + CN+PL +SF           P+  C+N++    ++PE P  R+  +D
Sbjct: 71  DSTYTSSSSHHIPCNTPLCNSF-----------PSNACSNNSSLCALFPENPVTRNTLLD 130

Query: 130 YLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLAALG 189
                       + D LAL T D   S SL  IS+  F+C   + L+GLA N +GLA+LG
Sbjct: 131 ----------TALIDSLALPTYDA--SSSLVLISDFIFSCATAHLLQGLAANALGLASLG 190

Query: 190 RANESIPLTISAKFNSPRIFAICLSGTRLGSGVA-FIGSKGPYAFSPNVDLSKSLIYTPL 249
           R+N S+P  IS    SPR F +CL  +   +G A F  +   + FS  +DL+    YT L
Sbjct: 191 RSNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLT----YTQL 250

Query: 250 LFNPQSGSIYT-NWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTY 309
           + NP + ++ T N  PS EY+I L++I+IN + +  N+S+L  +   G GG KIST+  Y
Sbjct: 251 IVNPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQT-GFGGTKISTAEPY 310

Query: 310 ALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEK 369
            +L++SIYR F   F+ E++  N T+  AVEPFGVC+    +  T  GP  P V L M  
Sbjct: 311 TVLETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHS 370

Query: 370 GKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRF 429
             V W++ G NSMVR+ K GVD+WCLG+V+GG   RTPIVIGG Q+ED+L+QFDL+  RF
Sbjct: 371 EDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRF 424

Query: 430 GFSSSALLQGTSCSKFNFSSSDN 449
           GF+S+ LLQ   CS    ++  N
Sbjct: 431 GFTSTLLLQDAKCSNLKVNNFAN 424

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BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.4e-75
Identity = 166/422 (39.34%), Postives = 234/422 (55.45%), Query Frame = 0


Query: 27  IAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYRLVKCNSPLS 86
           I PI KD  +  Y   +  +TPL P +L LDLGG F W+DC   Y SS+YR  +C S   
Sbjct: 35  ILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQC 94

Query: 87  DSFNHGV-CGSCIQAPTPICANDTIFSYVYPEKPSIRDEYVDYLNAKLTDSENVVTDVLA 146
                G  CG C   P P C N+T    + P+    R             S  + +D + 
Sbjct: 95  SLAGAGSGCGQCFSPPKPGCNNNT--CGLLPDNTITR----------TATSGELASDTVQ 154

Query: 147 LSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLAALGRANESIPLTISAKFNSPR 206
           + +++G   G      +  F C  T  L GLA  V G+A LGR   S+P   SA+F+ PR
Sbjct: 155 VQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPR 214

Query: 207 IFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS-LIYTPLLFNPQS-GSIYTNWLPSY 266
            FA+CLS +    GV   G  GPY F PN + + +   YTPL  NP S  S +++  PS 
Sbjct: 215 KFAVCLSSSTNSKGVVLFGD-GPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSS 274

Query: 267 EYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYALLQSSIYRAFAMAFMKE 326
           EY+IG+ +I+IN + V  NT+LL  +   G GG KIST   Y +L++SIY A    F+KE
Sbjct: 275 EYFIGVKSIKINEKVVPINTTLLSIDN-QGVGGTKISTVNPYTILETSIYNAVTNFFVKE 334

Query: 327 AAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKGKVVWKLGGRNSMVRIKK 386
             ++N T   +V PF  CF+  ++  T  GP  P + L ++   V W++ G NSMV++ +
Sbjct: 335 --LVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSE 394

Query: 387 LGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFGFSSSALLQGTSCSKFNF 446
              ++ CLG+V+GG  PRT IV+GG  +ED+LLQFDL + R GF+SS L + T+C+ FNF
Sbjct: 395 ---NVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFNF 437

[Close]

BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 1.8e-57
Identity = 145/455 (31.87%), Postives = 223/455 (49.01%), Query Frame = 0


Query: 5   LFFVFFTFSLLSFPFLSLQT-------AFIAPIHKDHNSGLYSISVHLKTPLRPANLHLD 64
           LFF   +  +L+      QT         +  + KD  + L+ + +H +TPL      +D
Sbjct: 10  LFFCSLSLIILTSSATKSQTHVPIRPNKLVLKVQKDRATNLHVVQIHKRTPLVQFPFVID 69

Query: 65  LGGAFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPE 124
           L G F  ++C N Y SS+Y+   C+S      N   C +C  + T               
Sbjct: 70  LTGRFLSVNCENQYTSSTYKAPVCHSSQCARANSHTCRTCSSSKT--------------- 129

Query: 125 KPSIRDEYVDYLNAKLTDSEN----VVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFL 184
           +P         L       ++    +  DVL + +T GS  G +    +  FAC  +N L
Sbjct: 130 RPGCHTNACGLLTTNPVTQQSAQGELAEDVLKIPSTQGSSPGPMVTYPHFLFACAPSNIL 189

Query: 185 -RGLARNVIGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFS 244
            +GL +NV G+A LG +  S+P  +++ F  P  FA+CL+ +   +G  F G +GPY   
Sbjct: 190 QKGLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSPGKNGAVFFG-EGPYFMK 249

Query: 245 PNVDLSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVH 304
           P +D+S+ L Y P     Q            EYYI + + +INN       ++L   P  
Sbjct: 250 PGIDVSRQLTYAPFTIGQQG-----------EYYINVQSFKINN-------AMLPSIPKG 309

Query: 305 GRGGAKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAA 364
           G GGA IST+T Y  LQ+ I+RA    FM +  +        V PFG CF+ + +  +  
Sbjct: 310 GFGGAMISTTTPYTTLQTPIFRALNQLFMNQ--LRGVPHVKPVAPFGACFDANRIPTSKM 369

Query: 365 GPRAPVVYLEME-KGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQM 424
           GP  P + L ++ K  ++W++ G N+M++ +     + CL +V+GG  P+ PIVIG  Q+
Sbjct: 370 GPTVPSIDLVLDNKKNIMWRIFGANAMIQPRP---GVMCLAFVDGGMRPKAPIVIGTQQL 425

Query: 425 EDHLLQFDLEKYRFGFSSSALLQGTSCSKFNFSSS 447
           ED+LLQFDL   R GFSSS L + T+C+ FNF +S
Sbjct: 430 EDNLLQFDLMNSRLGFSSSLLFRRTNCANFNFGTS 425

[Close]

BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-44
Identity = 136/431 (31.55%), Postives = 209/431 (48.49%), Query Frame = 0


Query: 27  IAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYRLVKCNSPLS 86
           + PI +D ++ L+  ++  +TPL    + LDL G   W+ C  HY+SS+Y+   C+S   
Sbjct: 49  VLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQC 108

Query: 87  DSFNHGVCGSCIQAPT--PICANDTIFSYVYPEKPSIRDEYVDYLNAKLTDSENVVTDVL 146
              N   C +C  + T  P C N+T    +    P  ++  +  L            DVL
Sbjct: 109 SRANTHQCFTCTDSTTSRPGCHNNT--CGLISSNPVTQESGLGEL----------AQDVL 168

Query: 147 ALSTTDGSRSGSLRRISNMPFACVKTNFL--RGLARNVIGLAALGRANESIPLTISAKFN 206
           AL +T GS+ GSL +I    F+C  T FL  +GL  NV G   LG A  S+P  + + F 
Sbjct: 169 ALHSTHGSKLGSLVKIPQFLFSCAPT-FLTQKGLPNNVQGALGLGHAPISLPNQLFSHFG 228

Query: 207 SPRIFAICLSGTRLGSGVAFIG-----SKGPYAFSPNVDLSKSLIYTPLLFNPQSGSIYT 266
             R F +CLS     +G    G     +   Y  + ++D+   ++YTPL  + Q      
Sbjct: 229 LKRQFTMCLSSYPTSNGAILFGDINDPNNNNYIHN-SLDVLHDMVYTPLTISKQG----- 288

Query: 267 NWLPSYEYYIGLSAIRIN--------NEAVRFNTSLLQFEPVHGRGGAKISTSTTYALLQ 326
                 EY+I +SAIR+N        N ++  ++S   +      GGA I+T+  Y +L+
Sbjct: 289 ------EYFIQVSAIRVNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLR 348

Query: 327 SSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKGKVV 386
            SI+  F   F             AV PFG+C++   +         P V L M+K  VV
Sbjct: 349 HSIFEVFTQVFANNVP--KQAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVV 408

Query: 387 WKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFGFSS 440
           W++ G N MV+ +  GV   CLG+V+GG   R  I +G  Q+E++L+ FDL + R GF++
Sbjct: 409 WRISGENLMVQAQD-GVS--CLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNT 444

[Close]

BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.9e-44
Identity = 134/440 (30.45%), Postives = 210/440 (47.73%), Query Frame = 0


Query: 16  SFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSS 75
           S P  S     + P+ +D ++GL+  ++H +TPL    L LDL G   W+ C  HY+SS+
Sbjct: 37  SQPTSSKPNLLVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSST 96

Query: 76  YRLVKCNSPLSDSFNHGVCGSCIQAPT--PICANDTIFSYVYPEKPSIRDEYVDYLNAKL 135
           Y+   C+S      N   C +C  + T  P C N+T    +    P  ++  +  L    
Sbjct: 97  YQAPFCHSTQCSRANTHQCFTCTDSTTTRPGCHNNT--CGLLSSNPVTQESGLGEL---- 156

Query: 136 TDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFL--RGLARNVIGLAALGRANE 195
                   DVLA+ +T GS+ G + ++    F+C   +FL  +GL  NV G   LG+A  
Sbjct: 157 ------AQDVLAIHSTHGSKLGPMVKVPQFLFSCA-PSFLAQKGLPNNVQGALGLGQAPI 216

Query: 196 SIPLTISAKFNSPRIFAICLSGTRLGSGVAFIG-----SKGPYAFSPNVDLSKSLIYTPL 255
           S+   + + F   R F++CLS     +G    G     +   Y  + ++D+   L+YTPL
Sbjct: 217 SLQNQLFSHFGLKRQFSVCLSRYSTSNGAILFGDINDPNNNNYIHN-SLDVLHDLVYTPL 276

Query: 256 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAV------RFNTSLLQFEPVHGRGGAKIS 315
             + Q            EY+I ++AIR+N   V        + S   +      GGA I+
Sbjct: 277 TISKQG-----------EYFIQVNAIRVNKHLVIPTKNPFISPSSTSYHGSGEIGGALIT 336

Query: 316 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 375
           T+  Y +L  SI+  F   F     M       AV PFG+C++   +        AP V 
Sbjct: 337 TTHPYTVLSHSIFEVFTQVFANN--MPKQAQVKAVGPFGLCYDSRKI-----SGGAPSVD 396

Query: 376 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 435
           L ++K   VW++   N MV+ +  GV   CLG+V+GG   R  I +G   +E++L+ FDL
Sbjct: 397 LILDKNDAVWRISSENFMVQAQD-GVS--CLGFVDGGVHARAGIALGAHHLEENLVVFDL 441

Query: 436 EKYRFGFSSSALLQ-GTSCS 440
           E+ R GF+S++L   G +CS
Sbjct: 457 ERSRVGFNSNSLKSYGKTCS 441

[Close]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL
Match: A0A6J1H501 (basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EKYRFGFSSSALLQGTSCSKFNFSSSDNFL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

[Close]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL
Match: A0A6J1KSD2 (basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 4.9e-252
Identity = 436/450 (96.89%), Postives = 441/450 (98.00%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPF SLQTAFIAPIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTP C NDTIF+YVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLR++S MPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNE VRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALLQSSIYRAFAMAFMKEAA LNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EKYRFGFSSSAL+QGTSCSKFNFSSSDNFL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

[Close]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL
Match: A0A5D3D4L4 (Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032G00050 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.8e-199
Identity = 348/452 (76.99%), Postives = 392/452 (86.73%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           M+TPL F FF F L+SFP  SLQTA +AP+ KDH S LYSIS+HLKTPLRPA L+LDLGG
Sbjct: 1   MSTPLLFFFF-FFLISFPSYSLQTASVAPLFKDHISLLYSISLHLKTPLRPATLYLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
            FSWIDCY +YNSSSY+L+ CN+PLS+SFN G+CGSC+QAP+P CANDTIF+Y YP+ PS
Sbjct: 61  PFSWIDCYQNYNSSSYKLLLCNTPLSNSFNQGICGSCVQAPSPTCANDTIFTYAYPQNPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           +RD++VDY   +LTDSENV+TDVLALSTTDGS+SG LRRIS +PFACVKTNFLRGLA+NV
Sbjct: 121 LRDQFVDYDRPELTDSENVITDVLALSTTDGSKSGPLRRISEIPFACVKTNFLRGLAKNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGR+N SIP  ISAKFNSP+ FAICLSG R G GVAF GSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRSNLSIPSVISAKFNSPKFFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           L YTPLLFNP SGSI+T WLPSYEYY+GLSAIRIN + V FNTSLL FEP+HG GGAKIS
Sbjct: 241 LTYTPLLFNPASGSIHTYWLPSYEYYVGLSAIRINGKVVPFNTSLLPFEPIHGNGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAG-PRAPVV 360
           TST Y LL+SSIYRAFA  FMKEAA LNF L NAV+PFGVC+   SV +TA G  +APVV
Sbjct: 301 TSTNYGLLESSIYRAFARVFMKEAAALNFKLINAVKPFGVCYAAKSVGVTAEGHAKAPVV 360

Query: 361 YLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFD 420
            L MEKGKVVWKLGGRN+MVRIKK GVD WCLG++NGGEFPRTPIV+GGLQMEDHLLQFD
Sbjct: 361 DLVMEKGKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVMGGLQMEDHLLQFD 420

Query: 421 LEKYRFGFSSSALLQGTSCSKFNFSS-SDNFL 451
           LEK+RFGFSSSAL +GTSCSKFNF+S ++NFL
Sbjct: 421 LEKFRFGFSSSALTEGTSCSKFNFNSINNNFL 451

[Close]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL
Match: A0A0A0K506 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G390050 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.5e-195
Identity = 343/449 (76.39%), Postives = 383/449 (85.30%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           M+TP  F FF F L+SFP  SLQTA IAP++K H S LYSIS+HLKTPLRPA+L+LDLGG
Sbjct: 1   MSTPPLFFFF-FFLISFPLYSLQTALIAPLYKHHTSLLYSISLHLKTPLRPASLYLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWI CY +YNSSSY+ V CN+PLS+SFN  +CGSC+QAP+PICANDTIFSY YPE PS
Sbjct: 61  AFSWIHCYQNYNSSSYKFVLCNTPLSNSFNQAICGSCVQAPSPICANDTIFSYAYPENPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           +RD +VDY + KLTDSENV+TDVLALSTT GS S  LRRI   PFACVKTNFLR +A+NV
Sbjct: 121 LRDHFVDYDHPKLTDSENVITDVLALSTTGGSTSAPLRRIPEFPFACVKTNFLREVAKNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGR+N SIP  ISAKF+SP+ FAICLSG R G GVAF GSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRSNLSIPSVISAKFSSPKYFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           L YTPLLFNP S SIYT WLPSYEYY+GLSAIRIN + V FNTSLL FEP+HGRGGAKIS
Sbjct: 241 LTYTPLLFNPVSASIYTYWLPSYEYYVGLSAIRINGKVVPFNTSLLSFEPIHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAG-PRAPVV 360
           TST YALL+SSIYRAFA  FMKEA +LNF L NAVEPFGVC+E  SV +TA G  +APVV
Sbjct: 301 TSTNYALLRSSIYRAFATVFMKEAVVLNFKLINAVEPFGVCYEAKSVGVTAEGQAKAPVV 360

Query: 361 YLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFD 420
            L MEK KVVWKLGGRN+MVRIKK GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFD
Sbjct: 361 DLVMEKEKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFD 420

Query: 421 LEKYRFGFSSSALLQGTSCSKFNFSSSDN 449
           LE +RFGFSSSAL +GTSCSKF+F+S++N
Sbjct: 421 LENFRFGFSSSALKEGTSCSKFDFTSANN 448

[Close]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL
Match: A0A6J1D7R8 (LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC111018418 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 3.3e-155
Identity = 282/409 (68.95%), Postives = 317/409 (77.51%), Query Frame = 0


Query: 45  LKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPI 104
           +KTPLRP  LHLDLGGAFSWIDCYNHYNSSSYRLV   S L +S +  + G C+ APTP 
Sbjct: 1   MKTPLRPTKLHLDLGGAFSWIDCYNHYNSSSYRLVDSESALCNSMHPNIAGPCVDAPTPF 60

Query: 105 CAND--TIFSYVYPEK-PSIRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRIS 164
           C+N+  T+F YVYPEK PSIRD  VDY + KLTDSEN++ DVLALSTTDGS  G LR+I 
Sbjct: 61  CSNNGTTLFCYVYPEKPPSIRDS-VDYDHVKLTDSENLIVDVLALSTTDGSNPGPLRQIP 120

Query: 165 NMPFACVKTNFLRGLARNVIGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVA 224
           ++P +C           +VIGL ALG ANESIP  +S  F   + FA+CL G R G GVA
Sbjct: 121 HLPISC----------XDVIGLVALGXANESIPSMVSTTFGRSKYFAVCLPGARSGPGVA 180

Query: 225 FIGSKGPYAFSPNVDLSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRF 284
           F GSKGPY FSPNVDLSKSLIYTPLLFNP S SIYT WLPSYEYYI LSAIRIN + V F
Sbjct: 181 FFGSKGPYKFSPNVDLSKSLIYTPLLFNPVSASIYTYWLPSYEYYIALSAIRINAKTVPF 240

Query: 285 NTSLLQFEPVHGRGGAKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVC 344
           NTSLL FEP+HG GG KISTS TYALLQ+SIYRAFA AF KE+A LNFT  + V+PFG+C
Sbjct: 241 NTSLLPFEPIHGGGGTKISTSATYALLQTSIYRAFAAAFAKESAALNFTATDPVKPFGLC 300

Query: 345 FEGSSVEMTAAGPRAPVVYLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPR 404
           +   SV MTAAGP AP V L ME GK  W+LGGRNSMVR+KK+GVD WCLG+++G E PR
Sbjct: 301 YAAESVAMTAAGPAAPAVDLVMEGGKSAWRLGGRNSMVRMKKVGVDAWCLGFIDGVENPR 360

Query: 405 TPIVIGGLQMEDHLLQFDLEKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           TPIVIGGLQMED LLQFDLE  RFGF SS LLQGTSCSKFNF+S DNF+
Sbjct: 361 TPIVIGGLQMEDQLLQFDLENSRFGFGSSVLLQGTSCSKFNFTSLDNFI 398

[Close]

BLAST of CmoCh08G003530 vs. NCBI nr
Match: XP_022959602.1 (basic 7S globulin 2-like [Cucurbita moschata])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EKYRFGFSSSALLQGTSCSKFNFSSSDNFL
Sbjct: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 450

[Close]

BLAST of CmoCh08G003530 vs. NCBI nr
Match: XP_023004536.1 (basic 7S globulin 2-like [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.0e-251
Identity = 436/450 (96.89%), Postives = 441/450 (98.00%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPF SLQTAFIAPIHKDHNS LYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTP C NDTIF+YVYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLR++S MPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNE VRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALLQSSIYRAFAMAFMKEAA LNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EKYRFGFSSSAL+QGTSCSKFNFSSSDNFL
Sbjct: 421 EKYRFGFSSSALMQGTSCSKFNFSSSDNFL 450

[Close]

BLAST of CmoCh08G003530 vs. NCBI nr
Match: KAG7027263.1 (hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 870.9 bits (2249), Expect = 4.7e-249
Identity = 435/450 (96.67%), Postives = 438/450 (97.33%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNH VC SCIQAPT ICAND IFS VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLN KLTDSENVVTDVLALSTTDGSR G LRRISNMPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRPGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALL+SSIYRAFA AFMKEAAMLNFTLANAVEPFGVCFEGSSV MTAAGPRAPVVY
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EK+RFGFSSSALLQGTSCSKFNFSSSDN L
Sbjct: 421 EKHRFGFSSSALLQGTSCSKFNFSSSDNLL 450

[Close]

BLAST of CmoCh08G003530 vs. NCBI nr
Match: KAG6593130.1 (putative aspartic proteinase GIP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 870.2 bits (2247), Expect = 8.0e-249
Identity = 434/450 (96.44%), Postives = 439/450 (97.56%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNH VC SCIQAPT ICAND IFS VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLN KLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARN+
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNI 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISA+FNSPRIFAICLSGTRLGSGVAFIGSKGPY FS NVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAEFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSTNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALL+SSIYRAFA AFMKEAAMLNFTLANAVEPFGVCFEGSSV MTAAGPRAPVVY
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EK+RFGFSSSALLQGTSCSKFNFSSSDN L
Sbjct: 421 EKHRFGFSSSALLQGTSCSKFNFSSSDNLL 450

[Close]

BLAST of CmoCh08G003530 vs. NCBI nr
Match: XP_023514746.1 (basic 7S globulin 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 869.8 bits (2246), Expect = 1.0e-248
Identity = 434/450 (96.44%), Postives = 438/450 (97.33%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60
           MATPLFFVFFTFSLL FPFLSLQTAFIAPIHKDHNSGLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLPFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120
           AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNH VC SCIQAPT ICAND IFS VYPEKPS
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPS 120

Query: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180
           IRDEYVDYLN KLTDSENVVTDVLALSTTDGSRSG LRRISNMPFACVKTNFLRGLARNV
Sbjct: 121 IRDEYVDYLNPKLTDSENVVTDVLALSTTDGSRSGLLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240
           IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPY FSPNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKS 240

Query: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300
           LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVY 360
           TSTTYALL+SSIYRAFA AFMKEAAMLNFTLANAVEPFGVCFEGSSV MTAAGPRAPVVY
Sbjct: 301 TSTTYALLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVY 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL
Sbjct: 361 LEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDL 420

Query: 421 EKYRFGFSSSALLQGTSCSKFNFSSSDNFL 451
           EK+RFGF+SSALLQGTSCSKFNFSSSDN L
Sbjct: 421 EKHRFGFTSSALLQGTSCSKFNFSSSDNLL 450

[Close]

BLAST of CmoCh08G003530 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-78
Identity = 173/443 (39.05%), Postives = 252/443 (56.88%), Query Frame = 0


Query: 5   LFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSW 64
           L F+F   S    PF     A + P+ KD ++  Y+  ++ +TPL PA++  DLGG   W
Sbjct: 12  LLFIFSLSSSAQTPF--RPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGRELW 71

Query: 65  IDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSIRDE 124
           +DC   Y SS+Y+  +CNS +        CG+C   P P C+N+T           I D 
Sbjct: 72  VDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTC--------GGIPDN 131

Query: 125 YVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLA 184
            V         S     DV+++ +T+GS  G + +I N+ F C  T  L+GLA+  +G+A
Sbjct: 132 TV----TGTATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMA 191

Query: 185 ALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKSLIYT 244
            +GR N  +P   +A F+  R FA+CL+    G GVAF G+ GPY F P + +S SL  T
Sbjct: 192 GMGRHNIGLPSQFAAAFSFHRKFAVCLTS---GKGVAFFGN-GPYVFLPGIQIS-SLQTT 251

Query: 245 PLLFNPQS-GSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTST 304
           PLL NP S  S ++    S EY+IG++AI+I  + V  N +LL+     G GG KIS+  
Sbjct: 252 PLLINPVSTASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGTKISSVN 311

Query: 305 TYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEM 364
            Y +L+SSIY AF   F+K+AA  +     +V+PFG CF   +V +T  G   P + L +
Sbjct: 312 PYTVLESSIYNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIELVL 371

Query: 365 EKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKY 424
               VVW++ G NSMV +     D+ CLG+V+GG   RT +VIGG Q+ED+L++FDL   
Sbjct: 372 HSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASN 431

Query: 425 RFGFSSSALLQGTSCSKFNFSSS 447
           +FGFSS+ L + T+C+ FNF+S+
Sbjct: 432 KFGFSSTLLGRQTNCANFNFTST 432

[Close]

BLAST of CmoCh08G003530 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 285.0 bits (728), Expect = 1.0e-76
Identity = 165/447 (36.91%), Postives = 253/447 (56.60%), Query Frame = 0


Query: 8   VFFTFSLLSFPFLSLQT-------AFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 67
           + F+  LLS   LS          A + P+ KD ++  Y+  ++ +TPL PA++  DLGG
Sbjct: 7   IIFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGG 66

Query: 68  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 127
              W+DC   Y S++YR  +CNS +        CG+C   P P C+N+T           
Sbjct: 67  REFWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTC---------- 126

Query: 128 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 187
               + D        S     DV+++ +T+GS  G   +I N+ F+C  T+ L+GLA+  
Sbjct: 127 --GAFPDNSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGA 186

Query: 188 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 247
           +G+A +GR N  +PL  +A F+  R FA+CL+    G GVAF G+ GPY F P + +S+ 
Sbjct: 187 VGMAGMGRHNIGLPLQFAAAFSFNRKFAVCLTS---GRGVAFFGN-GPYVFLPGIQISR- 246

Query: 248 LIYTPLLFNPQSGSI-YTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKI 307
           L  TPLL NP +    ++    S EY+IG++AI+I  + +  + +LL+     G GG KI
Sbjct: 247 LQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTKI 306

Query: 308 STSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVV 367
           S+   Y +L+SSIY+AF   F+++AA  +     +V+PFG CF   +V +T  G   P +
Sbjct: 307 SSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEI 366

Query: 368 YLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFD 427
            L +    VVW++ G NSMV +     D+ CLG+V+GG  P   +VIGG Q+ED+L++FD
Sbjct: 367 QLVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNPGASVVIGGFQLEDNLIEFD 426

Query: 428 LEKYRFGFSSSALLQGTSCSKFNFSSS 447
           L   +FGFSS+ L + T+C+ FNF+S+
Sbjct: 427 LASNKFGFSSTLLGRQTNCANFNFTST 433

[Close]

BLAST of CmoCh08G003530 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 164.5 bits (415), Expect = 2.0e-40
Identity = 130/446 (29.15%), Postives = 208/446 (46.64%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLR-PANLHLDLG 60
           M + L  +    S+ +   L   + ++ PI K   + L+  + ++ +  + P NL LDLG
Sbjct: 1   MGSSLTRLLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLG 60

Query: 61  GAFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCG--SCI-QAPTPICANDTIFSYVYP 120
              +W+DC    + SS RLV C S    S     C   SC+ + P P+  N  +      
Sbjct: 61  TNLTWLDCRKLKSLSSLRLVTCQSSTCKSIPGNGCAGKSCLYKQPNPLGQNPVV------ 120

Query: 121 EKPSIRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGL 180
                              +  VV D  +L TTDG +  S   + +  F+C     L+GL
Sbjct: 121 -------------------TGRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGL 180

Query: 181 ARNVIGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVD 240
              V G+ AL   + S    +++ FN    F++CL  +  G+G  +I   G + F P  +
Sbjct: 181 PPPVDGVLALSPGSSSFTKQVTSAFNVIPKFSLCLPSS--GTGHFYIA--GIHYFIPPFN 240

Query: 241 LSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGG 300
            S + I  P    P  G+       S +Y I + +I +   A++ N  LL        GG
Sbjct: 241 SSDNPI--PRTLTPIKGT------DSGDYLITVKSIYVGGTALKLNPDLL-------TGG 300

Query: 301 AKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSV-EMTAAGPR 360
           AK+ST   Y +LQ+ IY A A +F  +A  +      +V PF  CF+  +  +   AGP 
Sbjct: 301 AKLSTVVHYTVLQTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPN 360

Query: 361 APVVYLEM--EKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMED 420
            PV+ + +    G+V W   G N++V++K+    + CL +++GG+ P+  +VIG  Q++D
Sbjct: 361 VPVIEIGLPGRIGEVKWGFYGANTVVKVKE---TVMCLAFIDGGKTPKDLMVIGTHQLQD 399

Query: 421 HLLQFDLEKYRFGFSSSALLQGTSCS 440
           H+L+FD       FS S LL  TSCS
Sbjct: 421 HMLEFDFSGTVLAFSESLLLHNTSCS 399

[Close]

BLAST of CmoCh08G003530 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 139/443 (31.38%), Postives = 207/443 (46.73%), Query Frame = 0


Query: 12  FSLLSFPFLSLQT---------AFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAF 71
           F LLS  FL L           +F+ PI+KD    +Y+I + + +        LDL GA 
Sbjct: 7   FLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLNGAA 66

Query: 72  SWI-DCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSI 131
             + +C     S++Y  ++C S      N          P   C N+ I      +K ++
Sbjct: 67  PLLQNCPTAAKSTTYHPIRCGSTRCKYAN----------PNFPCPNNVI-----AKKRTV 126

Query: 132 RDEYVDYLNAKLTDSENVVTD-VLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 191
                       +D+  +  D V  L T +G  +      S++   C  T+    L +  
Sbjct: 127 ---------CLSSDNSRLFRDTVPLLYTFNGVYTRDSEMSSSLTLTC--TDGAPALKQRT 186

Query: 192 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLG---SGVAFIGSKGPYAFSP-NVD 251
           IGLA     + SIP  + + +  P   A+CL  T      +G  +IG KG Y + P + D
Sbjct: 187 IGLA---NTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIG-KGEYYYLPYDKD 246

Query: 252 LSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGG 311
           +SK    TPL+ N +SG          EY I + +I+I  + V          P+   G 
Sbjct: 247 VSKIFASTPLIGNGKSG----------EYLIDVKSIQIGAKTV----------PI-PYGA 306

Query: 312 AKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRA 371
            KIST   Y + Q+S+Y+A   AF +    +    A AV+PFG CF  +       G   
Sbjct: 307 TKISTLAPYTVFQTSLYKALLTAFTEN---IKIAKAPAVKPFGACFYSN------GGRGV 366

Query: 372 PVVYLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLL 431
           PV+ L +  G   W++ G NS+V++ K   ++ CLG+V+GG  P+ PIVIGG QMED+L+
Sbjct: 367 PVIDLVL-SGGAKWRIYGSNSLVKVNK---NVVCLGFVDGGVKPKYPIVIGGFQMEDNLV 384

Query: 432 QFDLEKYRFGFSSSALLQGTSCS 440
           +FDLE  +F FSSS LL  TSCS
Sbjct: 427 EFDLEASKFSFSSSLLLHNTSCS 384

[Close]

BLAST of CmoCh08G003530 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-33
Identity = 134/445 (30.11%), Postives = 195/445 (43.82%), Query Frame = 0


Query: 1   MATPLFFVFFTFSLLSFPFLSLQ------TAFIAPIHKDHNSGLYSISVHLKTPLRPANL 60
           MA+      F FS LS   +S           + P+ KD  +G Y   + L     P  L
Sbjct: 1   MASSSCLNLFFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKL 60

Query: 61  HLDLGGAFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYV 120
            +DL G+  W DC + + SSS  L+            G    C++A      N+ + S  
Sbjct: 61  VVDLAGSILWFDCSSRHVSSSRNLIS-----------GSSSGCLKAKV---GNERVSSSS 120

Query: 121 YPEKPSIRDEYVDYLNAK--LTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNF 180
              K    D  +   N    +T    + +DV+++ +   +  G++    ++ FAC     
Sbjct: 121 SSRKDQNADCELLVKNDAFGITARGELFSDVMSVGSV--TSPGTV----DLLFACTPPWL 180

Query: 181 LRGLARNVIGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFS 240
           LRGLA    G+  LGRA  S+P  ++A+ N  R   + LS     +GV    S       
Sbjct: 181 LRGLASGAQGVMGLGRAQISLPSQLAAETNERRRLTVYLSPL---NGVVSTSSVEEVF-- 240

Query: 241 PNVDLSKSLIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVH 300
             V  S+SL+YTPLL    +GS       S  Y I + +IR+N E +           V 
Sbjct: 241 -GVAASRSLVYTPLL----TGS-------SGNYVINVKSIRVNGEKL----------SVE 300

Query: 301 GRGGAKISTSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAA 360
           G    ++ST   Y +L+SSIY+ FA A+ K A     T    V PFG+CF  S V+    
Sbjct: 301 GPLAVELSTVVPYTILESSIYKVFAEAYAKAAG--EATSVPPVAPFGLCFT-SDVDF--- 360

Query: 361 GPRAPVVYLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQME 420
               P V L ++   V W++ G+N MV +   G  + C G V+GG     PIV+GGLQ+E
Sbjct: 361 ----PAVDLALQSEMVRWRIHGKNLMVDV---GGGVRCSGIVDGGSSRVNPIVMGGLQLE 385

Query: 421 DHLLQFDLEKYRFGFSSSALLQGTS 438
             +L FDL     GF        TS
Sbjct: 421 GFILDFDLGNSMMGFGQRTRSDSTS 385

The following BLAST results are available for this feature:

BLAST of CmoCh08G003530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1JNS6	8.7e-89	42.44	Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2	[more]
P0DO21	1.4e-75	39.34	Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ...	[more]
P82952	1.8e-57	31.87	Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2	[more]
Q9FSH9	1.3e-44	31.55	Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1	[more]
Q42369	2.9e-44	30.45	Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1	[more]

BLAST of CmoCh08G003530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H501	2.4e-259	100.00	basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1	[more]
A0A6J1KSD2	4.9e-252	96.89	basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1	[more]
A0A5D3D4L4	4.8e-199	76.99	Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032...	[more]
A0A0A0K506	5.5e-195	76.39	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G39005...	[more]
A0A6J1D7R8	3.3e-155	68.95	LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of CmoCh08G003530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022959602.1	5.0e-259	100.00	basic 7S globulin 2-like [Cucurbita moschata]	[more]
XP_023004536.1	1.0e-251	96.89	basic 7S globulin 2-like [Cucurbita maxima]	[more]
KAG7027263.1	4.7e-249	96.67	hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6593130.1	8.0e-249	96.44	putative aspartic proteinase GIP1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023514746.1	1.0e-248	96.44	basic 7S globulin 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh08G003530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03220.1	1.4e-78	39.05	Eukaryotic aspartyl protease family protein	[more]
AT1G03230.1	1.0e-76	36.91	Eukaryotic aspartyl protease family protein	[more]
AT5G19110.1	2.0e-40	29.15	Eukaryotic aspartyl protease family protein	[more]
AT5G19100.1	1.4e-36	31.38	Eukaryotic aspartyl protease family protein	[more]
AT5G19120.1	3.1e-33	30.11	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 21..225 e-value: 4.4E-39 score: 136.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 227..445 e-value: 1.9E-58 score: 199.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 31..437
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 264..428 e-value: 1.6E-44 score: 151.5
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 39..225 e-value: 3.5E-24 score: 86.0
None	No IPR available	PANTHER	PTHR47965:SF6	OS05G0403000 PROTEIN	coord: 7..443
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 7..443
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 39..428 score: 16.190859

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G003530.1	CmoCh08G003530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity