CmoCh08G002950 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G002950
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	hyoscyamine 6-dioxygenase-like
Location	Cmo_Chr08: 1813640 .. 1816045 (-)
RNA-Seq Expression	CmoCh08G002950
Synteny	CmoCh08G002950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTTTCAGTGCAAAATCAAATACAAATTATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACGCCATTCCTGTCCTCGATCTCTCAACCCATGATCGACCTCTTCTCGTTCGTAAAATCCTCGACGCTTCTCAAGAATTCGGATTTTTTCAGGTTTCAAATCTCTCCTTCGTGTTCTTCGATAATTCCAGTTCTTTTTCGATTATGATTGATTGTTTCGTTTCTTTTTGTTGTTGTTACGTACGGAACTATTTTGTTTTCATCTCGTGAAATTTGAGTTGGGTTTTAGAATTTTGATAATGTAAATGTTTAACGACCCACCTATATTTATAAATTTGAAAATATTTAAATTTTTAATTACGTATATATTGATCTAAATATAATTTACTTAATTAAGACATATATTGTAACGGCTCAAGTCCGCCGCCAGTAAATATTGTTTGTTTTTGCCGGTTACATATCGCCATCAGCTTCATGGTATTAAAACGTATCTACTAAAAAGAAGTTTCTACACCCTTTATAAGAAATGTTTCGTGTCAAACTCTAGCCGCCAGTAAATTTCGTATTTCCGCCTTGAAAAAACAATATATATATATATTTTTTACGTTTTTACTTGGGAATATGTCTCTTTTTCCCCTTTAGTTTCTAGTGTTTGTATGAATATGCCTTGGAAACTTACCTTTTTTACCATAATTTTGTAAAAAAAATTTAAATTAAAAACAACCCATTACATATTTTTACTATTTTCATCGAACATTTTAATATTTTGGATAGACAATCGATCGTTTTCAAATTAGGTCAAAATATAACTGAAGCTTAACCGTTAATTTTTTTTTAATTTAATTTTGCATCAGATAGAAACTCTTCGTATTTCATTGTCGGTCAGTTTTAATCTAAACCCATACTCGTAGTTTTTAATTATTAGATGAACTTTGTCACATCCAACCATTATTTTCGGTGAAAGATTGCACATGAATATAATTAATTCAACTTAAGAAAAGAAGAGAGAGGACGTGTTAACTTAAAAGCAAAATGTCAACTCTATTTAAGATTTAAAAGCTGGCAATAACAATTAATAATACTATAATTCATAAATTTTCAAATGTTTCGGTCTCGAGAATATATGACCTAATCAGTTAATTCTTGTTTTAGGTGATAAATCACGGAGTGTCGAGAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCATGCGATGTCGGGAGCTGAGAAGGTGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGGTGAAATTACATATATATCCTTTCCATTTTTACTAATTATCAATAATCTTTTTTATTTATAAATATAAAATTATTTTTAAAATATCACATAATTATTTATAAGCTCATAATTAAAAAAAATTGAAATTAATTATTGAGAAATAATTCAAGATAATTAATCGTCATTTAATAAATATTTAATTATGATATTGTTAACTTATTGCAGAGAAGTGGTCGGAGCGTACTGTATTGCGATGAGAACATTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTGGTTAACCATTACCCATCGTGTCCAAACCCTAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTCCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAATATTGGCTTCGTTCTACAGGTATGTTTAAAGTGAGTTTGACCTGTGTTTCTTAGAAGCTTTACTTATATGATGGTTGGTACGTAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACATCGAGTCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTAGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGTGATACCAAGGCAGTGATGGAGTGTATCGGCTCCGATCGCCGCTCTTAAAATTGTTTCGTTTTCTATGTTGAGTAATACCTTATTTCACGATGTAAACAAAATATCGTATAGTATCATCAAATAATATACTCC

mRNA sequence

TCTTTTCAGTGCAAAATCAAATACAAATTATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACGCCATTCCTGTCCTCGATCTCTCAACCCATGATCGACCTCTTCTCGTTCGTAAAATCCTCGACGCTTCTCAAGAATTCGGATTTTTTCAGGTGATAAATCACGGAGTGTCGAGAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCATGCGATGTCGGGAGCTGAGAAGGTGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGAGAAGTGGTCGGAGCGTACTGTATTGCGATGAGAACATTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTGGTTAACCATTACCCATCGTGTCCAAACCCTAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTCCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAATATTGGCTTCGTTCTACAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACATCGAGTCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTAGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGTGATACCAAGGCAGTGATGGAGTGTATCGGCTCCGATCGCCGCTCTTAAAATTGTTTCGTTTTCTATGTTGAGTAATACCTTATTTCACGATGTAAACAAAATATCGTATAGTATCATCAAATAATATACTCC

Coding sequence (CDS)

ATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACGCCATTCCTGTCCTCGATCTCTCAACCCATGATCGACCTCTTCTCGTTCGTAAAATCCTCGACGCTTCTCAAGAATTCGGATTTTTTCAGGTGATAAATCACGGAGTGTCGAGAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCATGCGATGTCGGGAGCTGAGAAGGTGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGAGAAGTGGTCGGAGCGTACTGTATTGCGATGAGAACATTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTGGTTAACCATTACCCATCGTGTCCAAACCCTAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTCCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAATATTGGCTTCGTTCTACAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACATCGAGTCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTAGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGTGATACCAAGGCAGTGATGGAGTGTATCGGCTCCGATCGCCGCTCTTAA

Protein sequence

MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS

Homology

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BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-68
Identity = 139/332 (41.87%), Postives = 195/332 (58.73%), Query Frame = 0


Query: 6   FVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFGF 65
           FVSNW   +SV   ++ P +KR     VP+ N +P++DL  H   LLV++I  A Q+FG 
Sbjct: 4   FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGL 63

Query: 66  FQVINHGVSRRVLEETTRIFKKFHAMSGAEKVK--------ECSRDPNKSCRVYTSSENY 125
           FQVINHG    ++ ET  + K+F A+   EK K        +      +  ++Y   E  
Sbjct: 64  FQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQL 123

Query: 126 RKEQTHCWRDALIFNCHPLDK-YVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGL 185
             E+   W+D L   CHPLD+  V+ WP+ P KYREVV  Y + +R L + +L+ + EGL
Sbjct: 124 SNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183

Query: 186 GLGRGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQ--VLKD 245
           GL  GY   E+S+  +ML N+YP CP+PS TLG   H D +LIT+L Q++ GLQ  ++KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243

Query: 246 GQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTME 305
             WI V PI  AFVVN+G  L+VI+N K + + HRVVT+    R S+  L+ P    T+E
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303

Query: 306 PAKCLINEANPPRYRALKFKDFQRNYLPRASD 327
           PAK L+N+ NPP Y+   + +F   YL   SD
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332

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BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 6.0e-66
Identity = 135/334 (40.42%), Postives = 198/334 (59.28%), Query Frame = 0


Query: 5   KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFG 64
           K +S      ++P +YV P   RP    V      P++DLS+ DR  L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHC 124
           FFQVINHGV++++++E   + ++F +MS  EK+K  S DP K+ R+ TS  N +KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R +  +I EL+SE LGL + Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSE-NPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E    M VN+YP CP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG  K+  HR VTN +  R S+   + P D   M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
             ++   P Y+   + ++ + +  R  D +  +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

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BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.6e-65
Identity = 129/321 (40.19%), Postives = 185/321 (57.63%), Query Frame = 0


Query: 15  SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFGFFQVINHGVS 74
           ++P  YV P  +RP   +V     IPV+DL++ DR  +V  + DA +  GFFQV+NHG+ 
Sbjct: 19  TMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGID 78

Query: 75  RRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNCH 134
             ++     + ++F  +   EK K  S DP K  R+ TS  N RKE  H WRD L  +C+
Sbjct: 79  AALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHCY 138

Query: 135 PLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMSENPL-M 194
           PL ++V  WP NPP ++E++G YC  +R L   + E +SE LGL  GY+   + E    M
Sbjct: 139 PLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHM 198

Query: 195 LVNHYPSCPNPSLTLGLSQHCDPSLITILF--QEVNGLQVLKDGQWIGVHPIDNAFVVNI 254
            VN+YP CP P LT GL  H DP+ +TIL    +V GLQVL DG+WI V+P   A V+NI
Sbjct: 199 AVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINI 258

Query: 255 GFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRAL 314
           G  LQ +SNGK ++  HR V N+   R S+   + P + V + PAK LI + +P  YR  
Sbjct: 259 GDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNY 318

Query: 315 KFKDFQRNYLPRASDTKAVME 333
            + ++ + +  R  D +  +E
Sbjct: 319 TYDEYYKKFWSRNLDQEHCLE 338

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BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 129/338 (38.17%), Postives = 193/338 (57.10%), Query Frame = 0


Query: 13  VQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLSTHD-----RPLLVRKILDASQEFGFF 72
           V  VP +YV P   RP  + V    ++IP++DL  HD     R  ++ +   A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCWR 132
           Q+ NHGV    +++     ++F   S +E+VK  S D  K+ R+ TS  N  KE+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGE 192
           D L  +C+P++ +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL +  +   
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPID 252
           + ++   M +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA- 312
           N F+VN+G  +QVISN K K+  HR V N+ + R S+     P ++  + PA+ LINE  
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 -NPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
            +P  YR   + ++   +   A DT++   CI S + S
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES---CIDSFKAS 346

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BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 120/309 (38.83%), Postives = 179/309 (57.93%), Query Frame = 0


Query: 15  SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFGFFQVINHGVS 74
           S+P  +V+  +K P         ++P++DLS   R  + R IL+A +E GFFQV+NHGVS
Sbjct: 13  SLPESFVFASDKVP--PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHGVS 72

Query: 75  RRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNC- 134
           ++V+ +   + ++F  +  A+K    S + +K  R++ S   Y       WRD L   C 
Sbjct: 73  KQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRLACP 132

Query: 135 HPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMS-ENPL 194
            P+D  ++ WP  P   R+V+  +    R +  E+L L+ EG+G+   Y  G++S  N +
Sbjct: 133 FPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGNVI 192

Query: 195 MLVNHYPSCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNAFVVNI 254
           + +NHYPSCPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFVVN 
Sbjct: 193 LNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNF 252

Query: 255 GFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRAL 314
           G  L+V++NG LK+ EHR +TN+ ++R S+   + P  E  + PAK  +++ NPP YR  
Sbjct: 253 GQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTT 312

Query: 315 KFKDFQRNY 321
            F+DF R Y
Sbjct: 313 MFRDFMRIY 317

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BLAST of CmoCh08G002950 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 4.3e-200
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 340

[Close]

BLAST of CmoCh08G002950 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.3e-192
Identity = 325/337 (96.44%), Postives = 330/337 (97.92%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDY+YPPEKRPGNVVVPMANAIPV+DLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEK KECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYL  EMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGS+
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

[Close]

BLAST of CmoCh08G002950 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.0e-169
Identity = 283/337 (83.98%), Postives = 309/337 (91.69%), Query Frame = 0


Query: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQ 61
           G  KFVSNWE V+SVP  YVYPPEKRPGN+VVPMA AIPV+DLS  DR LLVRKILDAS+
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121
           EFGFFQ+INHGVS++V EET RIFK+FHAMSG EK KECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
            HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           Y GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
           PIDNAFVVNIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

[Close]

BLAST of CmoCh08G002950 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.1e-169
Identity = 283/337 (83.98%), Postives = 309/337 (91.69%), Query Frame = 0


Query: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQ 61
           G+ KFVSNWE  +SVP  YVYPPEKRPGNVVVPMA AIPV+DLS  DR LLVRKIL AS+
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121
           E GFFQ+INHGVS+RV E T RIFK+FHAMSG EK KECS+DPN+SCRVYTSSENY+KEQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
            HCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           YLGGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
           PID AFVVNIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE TMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

[Close]

BLAST of CmoCh08G002950 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.7e-164
Identity = 270/335 (80.60%), Postives = 303/335 (90.45%), Query Frame = 0


Query: 3   EVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQE 62
           +V FVSNW GV+SVP  YVYPPEKRPGNV VP+ NAIPV+DL+ HDR  LV KILDASQE
Sbjct: 4   QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63

Query: 63  FGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQT 122
           FGFFQVINHGVS+ ++EET R+F++FHAMSG EK +ECS+DPN+SCRVYTSSEN+ KE+T
Sbjct: 64  FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123

Query: 123 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 182
           HCWRDALIFNCHPLDKYVHFWPQNP  YREVVGAYCI MR LV+EILEL+SEGLGLG+GY
Sbjct: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183

Query: 183 LGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
            G EMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243

Query: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
           +DNAFVVNIGFVLQV+SNGKLKA EHR V NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303

Query: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 338
           ANP  Y +L+FKDFQRNYLP A+DTKAV++ I SD
Sbjct: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSD 338

[Close]

BLAST of CmoCh08G002950 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 706.8 bits (1823), Expect = 8.9e-200
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 340

[Close]

BLAST of CmoCh08G002950 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 701.8 bits (1810), Expect = 2.8e-198
Identity = 338/340 (99.41%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 340

[Close]

BLAST of CmoCh08G002950 vs. NCBI nr
Match: KAG7025486.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.5 bits (1791), Expect = 4.5e-196
Identity = 334/340 (98.24%), Postives = 334/340 (98.24%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGV RRVLEETTRIFKKFHAMSGAEK KECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 340

[Close]

BLAST of CmoCh08G002950 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.3 bits (1775), Expect = 3.3e-194
Identity = 329/338 (97.34%), Postives = 331/338 (97.93%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEK KECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

[Close]

BLAST of CmoCh08G002950 vs. NCBI nr
Match: XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 680.6 bits (1755), Expect = 6.8e-192
Identity = 325/337 (96.44%), Postives = 330/337 (97.92%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDY+YPPEKRPGNVVVPMANAIPV+DLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEK KECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYL  EMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGS+
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

[Close]

BLAST of CmoCh08G002950 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.3e-67
Identity = 135/334 (40.42%), Postives = 198/334 (59.28%), Query Frame = 0


Query: 5   KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQEFG 64
           K +S      ++P +YV P   RP    V      P++DLS+ DR  L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHC 124
           FFQVINHGV++++++E   + ++F +MS  EK+K  S DP K+ R+ TS  N +KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R +  +I EL+SE LGL + Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSE-NPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E    M VN+YP CP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG  K+  HR VTN +  R S+   + P D   M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
             ++   P Y+   + ++ + +  R  D +  +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

[Close]

BLAST of CmoCh08G002950 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 8.6e-60
Identity = 129/338 (38.17%), Postives = 193/338 (57.10%), Query Frame = 0


Query: 13  VQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLSTHD-----RPLLVRKILDASQEFGFF 72
           V  VP +YV P   RP  + V    ++IP++DL  HD     R  ++ +   A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCWR 132
           Q+ NHGV    +++     ++F   S +E+VK  S D  K+ R+ TS  N  KE+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGE 192
           D L  +C+P++ +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL +  +   
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPID 252
           + ++   M +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA- 312
           N F+VN+G  +QVISN K K+  HR V N+ + R S+     P ++  + PA+ LINE  
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 -NPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
            +P  YR   + ++   +   A DT++   CI S + S
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES---CIDSFKAS 346

[Close]

BLAST of CmoCh08G002950 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 8.9e-57
Identity = 119/333 (35.74%), Postives = 188/333 (56.46%), Query Frame = 0


Query: 7   VSNWEGVQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLST---HDRPLLVRKILDASQE 66
           VS++     +P +YV P   RP  + V    ++IP++DL      +R ++V+++  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQT 126
           +GFFQ+ NHGV    + +   + ++F     +E+VK  S DP K+ R+ TS  N   ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 127 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 186
             WRD L  +C P++ ++  WP +P  +REV   Y  ++R LVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 LGGEMSENPL-MLVNHYPSCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGV 246
           +   + ++   M  N+YP CP P LT GL  H DP++IT+L Q +V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 306
            PI N F+VNIG  +QVISN K K+  HR V N +  R S+    +P  +  + PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NEANP-PRYRALKFKDFQRNYLPRASDTKAVME 333
           NE +    YR   F ++   +  R+  T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342

[Close]

BLAST of CmoCh08G002950 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.7e-53
Identity = 119/318 (37.42%), Postives = 174/318 (54.72%), Query Frame = 0


Query: 12  GVQSVPPDYVYPPEKRP--GNVVVPMANAIPVLDLSTHDRPLL----VRKILDASQEFGF 71
           GV  VP  YV PP +RP  G+ +      +PV+DLS   +P L    + +I  A +EFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 72  FQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCW 131
           FQVINHG+   V+ +      +F  +   EK+   S + ++  R Y +S N+  ++ H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGG 191
           RD +    HPL K++  WP NPP Y++ VG Y  A   L  +++E +SE LGL + YL  
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
           E+ E + +M VN YP+CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
           + A +V +G  ++V+SNG  K+  HRV  N ++ R S   L        + PA  L+N  
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322

Query: 312 NPPRYRALKFKDFQRNYL 322
           N P Y    F DF  NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338

[Close]

BLAST of CmoCh08G002950 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-50
Identity = 118/325 (36.31%), Postives = 171/325 (52.62%), Query Frame = 0


Query: 12  GVQSVPPDYVYPPEKRP--GNVVVPMANAIPVLDLSTHDRPLL----VRKILDASQEFGF 71
           G   VP  Y+ PP +RP  G  +      +PV+DLS    PLL    + +I  A + FGF
Sbjct: 23  GDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGF 82

Query: 72  FQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQTHCW 131
           FQVINHG+S  V+++      +F  +   EK+   S +  +  R Y +S N+  ++ H W
Sbjct: 83  FQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHYW 142

Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGG 191
           RD +    HPL  +++ WP NPP Y+E VG Y  A   L  +++E +SE LGL + YL  
Sbjct: 143 RDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
           E+ E + +M VN YP+CP P + LG+  H D   +TIL Q   GLQ+   +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
           + A +V +G  ++V+SNG  K+  HRV  N    R S   L        + PA  L+NE 
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNEN 322

Query: 312 NPPRYRALKFKDFQRNYLPRASDTK 329
            P  Y    F DF  +Y+ R   T+
Sbjct: 323 KPAAYGEFSFNDF-LDYISRNDITQ 345

The following BLAST results are available for this feature:

BLAST of CmoCh08G002950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24397	3.8e-68	41.87	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1	[more]
Q9FLV0	6.0e-66	40.42	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S...	[more]
Q8W2X5	6.6e-65	40.19	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]
Q9ZSA7	1.2e-58	38.17	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q40062	1.9e-56	38.83	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=...	[more]

BLAST of CmoCh08G002950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H5H6	4.3e-200	100.00	hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=...	[more]
A0A6J1KW36	3.3e-192	96.44	hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ...	[more]
A0A0A0K5H3	1.0e-169	83.98	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]
A0A1S3BR20	5.1e-169	83.98	hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1	[more]
A0A6J1CP90	1.7e-164	80.60	hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE...	[more]

BLAST of CmoCh08G002950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022959666.1	8.9e-200	100.00	hyoscyamine 6-dioxygenase-like [Cucurbita moschata]	[more]
KAG6593078.1	2.8e-198	99.41	Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7025486.1	4.5e-196	98.24	Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023514953.1	3.3e-194	97.34	hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004374.1	6.8e-192	96.44	hyoscyamine 6-dioxygenase-like [Cucurbita maxima]	[more]

BLAST of CmoCh08G002950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24530.1	4.3e-67	40.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	8.6e-60	38.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10500.1	8.9e-57	35.74	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G44800.1	2.7e-53	37.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G60290.1	2.8e-50	36.31	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 191..285 e-value: 1.1E-22 score: 80.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..327 e-value: 1.7E-87 score: 295.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 39..145 e-value: 6.0E-19 score: 68.9
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 3..333
None	No IPR available	PANTHER	PTHR47991:SF31	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 3..333
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..322
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 188..287 score: 12.28585

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G002950.1	CmoCh08G002950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding