CmoCh08G002680 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G002680
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDeoxyhypusine hydroxylase
LocationCmo_Chr08: 1674631 .. 1677856 (+)
RNA-Seq ExpressionCmoCh08G002680
SyntenyCmoCh08G002680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCACGATTTTCGCTCTGTGAAAAACTCTCCCTCCAATTTCCAGTTCTCTACGGAAGGTCCAATCTCATGGCCTTTGTCAATGACGACGCAGAAATCACCTCCTTCGCCTGCTCTTCAGAGATGGAGAAGTTCCTCTGCGACCGCTTGAACGACCCTTCTCAGCCCATTGCCGAGCGCTTCCGAGCTCTGTTCTCCCTCCGCAACCTCAAGGGCCCCGCCCCTCGCGATGCCCTCATTCTTGGTAAGCTCAATCATTTTTTTTTCTAAATTTTCCTGTGATTTGAAGTTCGAGTTGTGTTTAATCTGTTCATTTTTGTTCTTATTCCGTTTAATTGATTGTCGAAATAAATGCCTTGCAATTTCTTGGAAGTAGTCTGTTTAGTTATGCCCTCGGGGTTTGCTATCTGAGAATGGTAATCTGCTATGGAGTTCGTTGATATTGATTGATATTTGGTCGACGTCTTTATTTAACTTTTACGTCATGTTATTCTTTTAAACTTGCCTGCTATGGAGAGCCTGAGGCATATTTTTCACGTTACGATGTAAGGCCGGAAATATAATTAATCTAGAAGGGGAAAGAAAGAAACTCATTGTACGTACTTTTCTGTGCTTAGCGACTAGAGATCCTTCGAATTTGTTGGCTCATGAGGCTGCATTTGCATTGGGCCAAATGCAAGATACAGATGCAATCCCAGCGTTAGTGACTGTTCTTAACGATCTTTCTTTGCACCCCATTGTTCGGCACGAGGTGAAATTATAGAGCCCCCCTTGTTATCCTGCTTGTTTTGAATTTGATCTAGTGTTCTGTTCTTCCATCTAACCTGTTGTATTTGTTTCTGTAATGGTGTAGGCAGCAGAAGCTCTTGGAGCTATTGGCTCAGAGAGTAATATTCCTTATTTGGAAAAGAGCTTGGCTTTAGATCCTGCTCAAGAGGTTAAAGAGACATGTGAATTGGCCCTTAAGAGAATTGAACAACTGAAAGATTCTAGTAAAGAAGGTGAATCATCCACAGTTGAAAAGTCACCATTTCTGTCTGTCGATCCAGCTGCCCCAGCTTCTTCTGATTCTTCAGTATGTCAACTTAGGTATGTCGAGGCAATTGTAATAAATGCTATATACTATCAACCTTCTATAATAAGGATGTTGCTTGATAGTAGCTTGTAGTAATGGTTTCTGATTACTTTTACTACCTCATTTATCCATTTGAAGGGAAATTCTTCTGGATGATGATAAGGGCATGTATGAGCGCTATGCAGCCCTTTTTGCTTTACGGAATAATGGCAACGATGAAGCTTTAACTGCAATTATCAACTCTTTAAGGTCCAAAAGTGCTCTGCTAAAGCATGAGGTTTGTTTTAATGACCCATAATTTTATTTCACCATATTAACGTGGATTGTAATTTCTTAATAATCATATGTCATTTTTCTTTATTCTCTTTTCGAACTTAGTCTGATCTTGAATTCTTAATTCTTTTACCTTTGGAAACAAACCTAACAGATTTTTGGACATTAATTTAGTTTGAATGCTCAGCATGCCATGCCACTTTGTGGAGACGCCATTTCCTGATTTGATGTTGCTACTTTCTTGAATTATTAGGTTGCTTATGTATTAGGCCAATTGCAAAACAAAGCAGCTTCAGATGCTCTCTCCAATATTCTTGAGGATGTTAATGAGCATCCAATGGTTAGACATGAGGCTGCTGAAGCTCTTGGTTCCATTGCAGGTATAATTTATTTGTTGTTTTGTTAAACATGCTTGTTCTTTTGGAACCCAAAAGTATTATTTTTTTCCTTCTTCACCTTCTGATTTAAATTTAGAAAGAAAATACAACCGGACTATGTGCTTTGTAAAGAATCCTGAAAAAGTGATCTGTTATTGTAATGTGATAGGTCTTAGTTTTAGTTATTATAAATAACATAAGAATTCATTTGCATACTATCCAAGTAACTAGTATGTTTCGATCCTTCATTTTTTGACTATCAAGTCATATAAGCTCTTTCAAGACTCAAGAGTGGGGTACAAAAGGGAGGTGTATATTTTTTCTGATGAGGCACACAAAATATGATCCAAATAAGATAACTATTTTATAAATAGAAAACATAGCATCCTGAAACGTGAGCATTACAATAAATCAAACAAATTGGAGCTTTTTCTGAGAGGCTGTTTAGAAATGTAGGCATCTAATTTTAAGGGGAAGAAAACTTTCAAACTGAAGGCAGAGTGGCACGCCCCTTGGTGAGGTTGCTTGCCATGAGTATAAGAAAAATCCTTGATGCTTCCATACTCTAGGCCTAGACGTGTGGATTTTTGTAAGGTGTGTGTGTGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTTGGTCCAATAATCGTCTGAAAAGCTTGTTGAGATCCTTTATTAAATGTTTCTTACTACAAAATTCAATATAAAAAACAGGACTTAAACCTTATCTTTACTGAGTTACCTATTAGGACGTTAGTTCATGAATAGTAGAACTATGCTGGAACATGAAATTCAAGTCCAGCGGTAACTGATCAGAAGAAGGATATTTTGTTACGCTAAATAACAAGTTATAGGCGGTGCCTCAGTTGCTGTGTGCTTCTTTATTAATTGGATTCTTATTTTGTTTTCATTGTATTTGTAGACGAAAGAAGCATAGCACTTCTCAAGGAGTTTGCAAAGGATCCTGAGCCTATTGTCTCGCAGAGTTGTGAGGTTGCACTAAGCATGCTGGAGTATGAAAGGTTGGGAAGATCATTTGAGGTATACATTCTGGACTCCCTGACCATACTTTAATGGATACGTTTTTCTATATTGTCTAATTAAAATACACCTGTAAGTAACTTAAGCTAATGCACCCTGCAGTTCCTGTTCATGCAATCTCCTCAAGTGCAATAAGTTGCAACAAACAGAAAATAGCATCAATTGCGAAGGTTAGAATCAGCCCCATCTCTTGTTTGTCTACAACAAATTGATATAGTTAGAAACTGAGAGCTTGTTTAGTTTTATAGGTGATATGCCTTTGACTTAGTGGAGATAGTCAAGATTGGAGCAATATAGACAGTAATTATCTATGTTTATTGTTCCATTTGGATATATCTAAACTTTCCTCAATTGGAATGAAAGATACAGAAATCTTACCAAAAAAGATATTGAAAATTATATATTTTCTCA

mRNA sequence

CCTTCACGATTTTCGCTCTGTGAAAAACTCTCCCTCCAATTTCCAGTTCTCTACGGAAGGTCCAATCTCATGGCCTTTGTCAATGACGACGCAGAAATCACCTCCTTCGCCTGCTCTTCAGAGATGGAGAAGTTCCTCTGCGACCGCTTGAACGACCCTTCTCAGCCCATTGCCGAGCGCTTCCGAGCTCTGTTCTCCCTCCGCAACCTCAAGGGCCCCGCCCCTCGCGATGCCCTCATTCTTGCGACTAGAGATCCTTCGAATTTGTTGGCTCATGAGGCTGCATTTGCATTGGGCCAAATGCAAGATACAGATGCAATCCCAGCGTTAGTGACTGTTCTTAACGATCTTTCTTTGCACCCCATTGTTCGGCACGAGGCAGCAGAAGCTCTTGGAGCTATTGGCTCAGAGAGTAATATTCCTTATTTGGAAAAGAGCTTGGCTTTAGATCCTGCTCAAGAGGTTAAAGAGACATGTGAATTGGCCCTTAAGAGAATTGAACAACTGAAAGATTCTAGTAAAGAAGGTGAATCATCCACAGTTGAAAAGTCACCATTTCTGTCTGTCGATCCAGCTGCCCCAGCTTCTTCTGATTCTTCAGTATGTCAACTTAGGGAAATTCTTCTGGATGATGATAAGGGCATGTATGAGCGCTATGCAGCCCTTTTTGCTTTACGGAATAATGGCAACGATGAAGCTTTAACTGCAATTATCAACTCTTTAAGGTCCAAAAGTGCTCTGCTAAAGCATGAGGTTGCTTATGTATTAGGCCAATTGCAAAACAAAGCAGCTTCAGATGCTCTCTCCAATATTCTTGAGGATGTTAATGAGCATCCAATGGTTAGACATGAGGCTGCTGAAGCTCTTGGTTCCATTGCAGACGAAAGAAGCATAGCACTTCTCAAGGAGTTTGCAAAGGATCCTGAGCCTATTGTCTCGCAGAGTTGTGAGGTTGCACTAAGCATGCTGGAGTATGAAAGGTTGGGAAGATCATTTGAGTTCCTGTTCATGCAATCTCCTCAAGTGCAATAAGTTGCAACAAACAGAAAATAGCATCAATTGCGAAGGTTAGAATCAGCCCCATCTCTTGTTTGTCTACAACAAATTGATATAGTTAGAAACTGAGAGCTTGTTTAGTTTTATAGGTGATATGCCTTTGACTTAGTGGAGATAGTCAAGATTGGAGCAATATAGACAGTAATTATCTATGTTTATTGTTCCATTTGGATATATCTAAACTTTCCTCAATTGGAATGAAAGATACAGAAATCTTACCAAAAAAGATATTGAAAATTATATATTTTCTCA

Coding sequence (CDS)

ATGGCCTTTGTCAATGACGACGCAGAAATCACCTCCTTCGCCTGCTCTTCAGAGATGGAGAAGTTCCTCTGCGACCGCTTGAACGACCCTTCTCAGCCCATTGCCGAGCGCTTCCGAGCTCTGTTCTCCCTCCGCAACCTCAAGGGCCCCGCCCCTCGCGATGCCCTCATTCTTGCGACTAGAGATCCTTCGAATTTGTTGGCTCATGAGGCTGCATTTGCATTGGGCCAAATGCAAGATACAGATGCAATCCCAGCGTTAGTGACTGTTCTTAACGATCTTTCTTTGCACCCCATTGTTCGGCACGAGGCAGCAGAAGCTCTTGGAGCTATTGGCTCAGAGAGTAATATTCCTTATTTGGAAAAGAGCTTGGCTTTAGATCCTGCTCAAGAGGTTAAAGAGACATGTGAATTGGCCCTTAAGAGAATTGAACAACTGAAAGATTCTAGTAAAGAAGGTGAATCATCCACAGTTGAAAAGTCACCATTTCTGTCTGTCGATCCAGCTGCCCCAGCTTCTTCTGATTCTTCAGTATGTCAACTTAGGGAAATTCTTCTGGATGATGATAAGGGCATGTATGAGCGCTATGCAGCCCTTTTTGCTTTACGGAATAATGGCAACGATGAAGCTTTAACTGCAATTATCAACTCTTTAAGGTCCAAAAGTGCTCTGCTAAAGCATGAGGTTGCTTATGTATTAGGCCAATTGCAAAACAAAGCAGCTTCAGATGCTCTCTCCAATATTCTTGAGGATGTTAATGAGCATCCAATGGTTAGACATGAGGCTGCTGAAGCTCTTGGTTCCATTGCAGACGAAAGAAGCATAGCACTTCTCAAGGAGTTTGCAAAGGATCCTGAGCCTATTGTCTCGCAGAGTTGTGAGGTTGCACTAAGCATGCTGGAGTATGAAAGGTTGGGAAGATCATTTGAGTTCCTGTTCATGCAATCTCCTCAAGTGCAATAA

Protein sequence

MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFMQSPQVQ
Homology
[Close]
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match: Q94JW0 (Deoxyhypusine hydroxylase OS=Arabidopsis thaliana OX=3702 GN=At3g58180 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.9e-122
Identity = 227/301 (75.42%), Postives = 263/301 (87.38%), Query Frame = 0 

Query: 19  MEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQM 78
           +EKFLC+RL D SQPI+ERFRALFSLRNLKGP PR+ALILA+RD SNLLAHEAAFALGQM
Sbjct: 13  LEKFLCERLVDQSQPISERFRALFSLRNLKGPGPRNALILASRDSSNLLAHEAAFALGQM 72

Query: 79  QDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCEL 138
           QD +AIPAL +VLND+SLHPIVRHEAAEALGAIG   N+  L+KSL+ DPAQEV+ETCEL
Sbjct: 73  QDAEAIPALESVLNDMSLHPIVRHEAAEALGAIGLAGNVNILKKSLSSDPAQEVRETCEL 132

Query: 139 ALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAA 198
           ALKRIE + +   E +SST EKSPF+SVDPA PA+S SSV QLR++LLD+ KGMYERYAA
Sbjct: 133 ALKRIEDMSNVDAENQSSTTEKSPFMSVDPAGPAASFSSVHQLRQVLLDETKGMYERYAA 192

Query: 199 LFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMV 258
           LFALRN+G +EA++AI++SL + SALL+HEVAYVLGQLQ+K A   LS +L DVNEHPMV
Sbjct: 193 LFALRNHGGEEAVSAIVDSLSASSALLRHEVAYVLGQLQSKTALATLSKVLRDVNEHPMV 252

Query: 259 RHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFMQSPQ 318
           RHEAAEALGSIADE+SIALL+EF+KDPEPIV+QSCEVALSMLE+E  G+SFEF F Q P 
Sbjct: 253 RHEAAEALGSIADEQSIALLEEFSKDPEPIVAQSCEVALSMLEFENSGKSFEFFFTQDPL 312

Query: 319 V 320
           V
Sbjct: 313 V 313

[Close]
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match: Q2QXB3 (Deoxyhypusine hydroxylase-A OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0163300 PE=2 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.4e-119
Identity = 232/313 (74.12%), Postives = 263/313 (84.03%), Query Frame = 0 

Query: 8   AEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLL 67
           A  ++F  + EMEKFLCD L D +QPIAERFRALFSLRNL G  PR AL+ A RD SNLL
Sbjct: 7   AASSTFGPTPEMEKFLCDLLLDTAQPIAERFRALFSLRNLHGDGPRCALLQAARDSSNLL 66

Query: 68  AHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALD 127
           AHEAAFALGQMQD +AIPAL  VL DLSLHPIVRHEAAEALGAIG E +IP LE+SLA D
Sbjct: 67  AHEAAFALGQMQDAEAIPALEAVLKDLSLHPIVRHEAAEALGAIGLEKSIPLLEESLAAD 126

Query: 128 PAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLD 187
           PA EV+ETCELAL+RIEQ K++   G S +   SPFLSVDPA PA    SV QLREILL+
Sbjct: 127 PAVEVQETCELALRRIEQQKNA---GVSESTTISPFLSVDPALPAKQGLSVHQLREILLN 186

Query: 188 DDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSN 247
           + + MYERYAALFALRN+  D A++AI+ +L +KSALLKHEVAYVLGQLQNKAASDALS 
Sbjct: 187 EQESMYERYAALFALRNDSRDAAVSAIVAALGAKSALLKHEVAYVLGQLQNKAASDALST 246

Query: 248 ILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGR 307
           +L++V+EHPMVRHEAAEALGSIAD+ SIALL+EFAKDPEPIVSQSCEVALSMLEYER G+
Sbjct: 247 VLKNVDEHPMVRHEAAEALGSIADQESIALLEEFAKDPEPIVSQSCEVALSMLEYERSGK 306

Query: 308 SFEFLFMQSPQVQ 321
           SFEFLF+Q+P VQ
Sbjct: 307 SFEFLFLQTPGVQ 316

[Close]
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match: Q2QLW3 (Deoxyhypusine hydroxylase-B OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0626100 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.5e-116
Identity = 227/302 (75.17%), Postives = 260/302 (86.09%), Query Frame = 0 

Query: 19  MEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQM 78
           ME FLCDRL D +QPIAERFRALFSLRNL+G APR AL+ A RD SNLLAHEAAFALGQM
Sbjct: 1   MEGFLCDRLLDAAQPIAERFRALFSLRNLRGDAPRRALLQAARDSSNLLAHEAAFALGQM 60

Query: 79  QDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCEL 138
           QD +AIPAL  VL DLSLHPIVRHEAAEALGAIG E +I  LE+SLA+DPA EV+ETCEL
Sbjct: 61  QDAEAIPALEAVLKDLSLHPIVRHEAAEALGAIGLEKSISLLEESLAVDPAVEVQETCEL 120

Query: 139 ALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAA 198
           A++RIE+ K++S   ES+TV  SPFLSVDPA PA     V QLRE+LL++ + MYERYAA
Sbjct: 121 AIRRIEEQKNTSGV-ESATV--SPFLSVDPALPAKQGLPVEQLRELLLNEQESMYERYAA 180

Query: 199 LFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMV 258
           LFALRN+  D A++AI+ +L  KSALL+HEVAYVLGQLQNKAASDALS +L++V+EHPMV
Sbjct: 181 LFALRNDSGDAAVSAIVAALGVKSALLRHEVAYVLGQLQNKAASDALSTVLKNVDEHPMV 240

Query: 259 RHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFMQSPQ 318
           RHEAAEALGSIAD+ SIALL+EFAKDPEPIVSQSCEVALSMLEYER G++FEFLF Q+PQ
Sbjct: 241 RHEAAEALGSIADQESIALLEEFAKDPEPIVSQSCEVALSMLEYERSGKAFEFLFPQTPQ 299

Query: 319 VQ 321
           VQ
Sbjct: 301 VQ 299

[Close]
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match: Q5ZIP3 (Deoxyhypusine hydroxylase OS=Gallus gallus OX=9031 GN=DOHH PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.2e-65
Identity = 142/285 (49.82%), Postives = 189/285 (66.32%), Query Frame = 0 

Query: 27  LNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQMQDTDAIPA 86
           L D +QP+  RFRALF+LRNL GPA  D ++    D S LL HE AF LGQM+D  AIPA
Sbjct: 14  LLDAAQPLPARFRALFTLRNLGGPAAIDCIVRGFADSSALLKHELAFCLGQMRDRAAIPA 73

Query: 87  LVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCELALKRIEQL 146
           L+ VL D    P+VRHEA EALGAIG    +  L +  + DP  EV ETC+LA++R+E L
Sbjct: 74  LLGVLQDSQQEPMVRHEAGEALGAIGDPEVLDVLRR-YSEDPVVEVAETCQLAVRRLEWL 133

Query: 147 KDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAALFALRNNG 206
           ++  +E  S     SP+ SVDPA PA  ++ V  LR +LLD+ + +++RY A+FALRN G
Sbjct: 134 QEHGEEPGS-----SPYRSVDPAPPA-EETDVATLRAVLLDESRPLFDRYRAMFALRNLG 193

Query: 207 NDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMVRHEAAEAL 266
             +A+ A+ + LR+ SAL +HE+ YVLGQ+Q++A    L+  L    E+PMVRHE AEAL
Sbjct: 194 GRDAVLALADGLRAGSALFRHEIGYVLGQMQDEACVPQLTAALRSRAENPMVRHECAEAL 253

Query: 267 GSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEF 312
           GSIA    +  L+ FA+D E +V +SCEVAL M EYE  G  F++
Sbjct: 254 GSIARPSCLETLRAFAQDEERVVRESCEVALDMYEYEN-GPQFQY 290

[Close]
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match: Q9BU89 (Deoxyhypusine hydroxylase OS=Homo sapiens OX=9606 GN=DOHH PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.0e-64
Identity = 146/285 (51.23%), Postives = 183/285 (64.21%), Query Frame = 0 

Query: 27  LNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQMQDTDAIPA 86
           L DP QP+  RFRALF+LR L GP     +  A  D S LL HE A+ LGQMQD  AIP 
Sbjct: 14  LVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPM 73

Query: 87  LVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCELALKRIEQL 146
           LV VL D    P+VRHEA EALGAIG    +  L K  + DP  EV ETC+LA++R+E L
Sbjct: 74  LVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEIL-KQYSSDPVIEVAETCQLAVRRLEWL 133

Query: 147 KDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAALFALRNNG 206
           +    E  +      P+LSVDPA PA  +  V +LRE LLD+ + ++ERY A+FALRN G
Sbjct: 134 QQHGGEPAA-----GPYLSVDPAPPA-EERDVGRLREALLDESRPLFERYRAMFALRNAG 193

Query: 207 NDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMVRHEAAEAL 266
            +EA  A+   L   SAL +HEV YVLGQLQ++AA   L+  L    E+PMVRHE AEAL
Sbjct: 194 GEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEAL 253

Query: 267 GSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEF 312
           G+IA    +A L+  A DPE +V +SCEVAL M E+E  GR+F++
Sbjct: 254 GAIARPACLAALQAHADDPERVVRESCEVALDMYEHE-TGRAFQY 290

[Close]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match: A0A6J1H786 (Deoxyhypusine hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111460813 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.9e-171
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT
Sbjct: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL
Sbjct: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match: A0A6J1KPZ8 (Deoxyhypusine hydroxylase OS=Cucurbita maxima OX=3661 GN=LOC111497653 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.3e-169
Identity = 316/320 (98.75%), Postives = 318/320 (99.38%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVN DAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT
Sbjct: 37  MAFVNGDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 96

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIP+L
Sbjct: 97  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPFL 156

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSS +GESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 157 EKSLALDPAQEVKETCELALKRIEQLKDSSNDGESSTVEKSPFLSVDPAAPASSDSSVCQ 216

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 217 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 276

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML
Sbjct: 277 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 336

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 337 EYERLGRSFEFLFMQSPQVQ 356

[Close]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match: A0A1S3BGF3 (Deoxyhypusine hydroxylase OS=Cucumis melo OX=3656 GN=LOC103489734 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.7e-151
Identity = 285/320 (89.06%), Postives = 301/320 (94.06%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MA VN   +++ F+CSS+MEKFLCDRLNDPS PIA+RFRALFSLRNLKGPAPR+ALI+AT
Sbjct: 1   MASVNGHGDVSFFSCSSDMEKFLCDRLNDPSLPIADRFRALFSLRNLKGPAPRNALIIAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RD SNLLAHEAAFALGQMQD DAIPALV VLNDLSLHPIVRHEAAEALGAIG ESNIP+L
Sbjct: 61  RDSSNLLAHEAAFALGQMQDADAIPALVAVLNDLSLHPIVRHEAAEALGAIGLESNIPFL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELAL+RIEQLKDS  E ESSTVEKSPFLSVDPAAPASS +SV Q
Sbjct: 121 EKSLALDPAQEVKETCELALRRIEQLKDSGNEDESSTVEKSPFLSVDPAAPASSSASVGQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLD+DKGMYERYAALFALRNNGNDEALTAII+SL S SALLKHEVAYVLGQLQ KA
Sbjct: 181 LREILLDEDKGMYERYAALFALRNNGNDEALTAIIDSLSSTSALLKHEVAYVLGQLQKKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALS+ILEDV EHPMVRHEAAEALGSIADE+SIALLKEF+KDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSDILEDVKEHPMVRHEAAEALGSIADEKSIALLKEFSKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match: A0A0A0K8K5 (Deoxyhypusine hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_7G372960 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.7e-150
Identity = 285/320 (89.06%), Postives = 300/320 (93.75%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MA VN D +++SF+CSS+MEKFLCDRLNDPS PIAERFRALFSLRNLKGPAPR+ALILAT
Sbjct: 1   MASVNGDGDVSSFSCSSDMEKFLCDRLNDPSFPIAERFRALFSLRNLKGPAPRNALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RD SNLLAHEAAFALGQMQD DAIPALV VLNDLSLHPIVRHEAAEALGAIG ESNIP+L
Sbjct: 61  RDSSNLLAHEAAFALGQMQDADAIPALVAVLNDLSLHPIVRHEAAEALGAIGLESNIPFL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELAL+RIEQLKDS  E ESS VEKSPFLSVDPAAPASS +SV Q
Sbjct: 121 EKSLALDPAQEVKETCELALRRIEQLKDSGNEDESSKVEKSPFLSVDPAAPASSSASVGQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLD+DK MYERYAALF LRNNGNDEALTAII+SL S SALLKHEVAYVLGQLQ KA
Sbjct: 181 LREILLDEDKDMYERYAALFTLRNNGNDEALTAIIDSLSSTSALLKHEVAYVLGQLQKKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALS+ILEDV EHPMVRHEAAEALGSIADE+SIALLKEF+KDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSDILEDVKEHPMVRHEAAEALGSIADEKSIALLKEFSKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLG+SFEFLFMQSPQVQ
Sbjct: 301 EYERLGKSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match: A0A6J1DQT9 (Deoxyhypusine hydroxylase OS=Momordica charantia OX=3673 GN=LOC111023477 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 5.0e-150
Identity = 284/306 (92.81%), Postives = 297/306 (97.06%), Query Frame = 0 

Query: 15  CSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFA 74
           CSSEMEKFLCDRLNDP+QPIAERFRALFSLRNLKGPAPRDALILATRD SNLLAHEAAFA
Sbjct: 9   CSSEMEKFLCDRLNDPTQPIAERFRALFSLRNLKGPAPRDALILATRDSSNLLAHEAAFA 68

Query: 75  LGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKE 134
           LGQMQDTD+IPALV+VLNDLSLHPIVRHEAAEALGAIG ESNIP L+KSLALDPAQEVKE
Sbjct: 69  LGQMQDTDSIPALVSVLNDLSLHPIVRHEAAEALGAIGLESNIPLLKKSLALDPAQEVKE 128

Query: 135 TCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYE 194
           TCELALKRIEQLKDS  EGESST+EKSPF+SVDPAAPASS++SV QLREILLDDDKGMYE
Sbjct: 129 TCELALKRIEQLKDSGNEGESSTIEKSPFMSVDPAAPASSNASVHQLREILLDDDKGMYE 188

Query: 195 RYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNE 254
           RYAALFALRNNGNDEALTAII+SL S SALLKHEVAYVLGQLQ+KAAS ALS+ILE+VNE
Sbjct: 189 RYAALFALRNNGNDEALTAIIDSLSSTSALLKHEVAYVLGQLQDKAASYALSDILENVNE 248

Query: 255 HPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFM 314
           HPMVRHEAAEALGSIADERSIALLK+FAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFM
Sbjct: 249 HPMVRHEAAEALGSIADERSIALLKKFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFM 308

Query: 315 QSPQVQ 321
           QSPQVQ
Sbjct: 309 QSPQVQ 314

[Close]
BLAST of CmoCh08G002680 vs. NCBI nr
Match: XP_022959892.1 (deoxyhypusine hydroxylase [Cucurbita moschata] >XP_022959893.1 deoxyhypusine hydroxylase [Cucurbita moschata])

HSP 1 Score: 610.5 bits (1573), Expect = 8.1e-171
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT
Sbjct: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL
Sbjct: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. NCBI nr
Match: KAG6593054.1 (Deoxyhypusine hydroxylase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025462.1 Deoxyhypusine hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.0 bits (1556), Expect = 7.6e-169
Identity = 317/320 (99.06%), Postives = 317/320 (99.06%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVN DAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT
Sbjct: 1   MAFVNGDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALV VLNDLSLHPIVRHEAAEALGAIGSESNIPYL
Sbjct: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVNVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSS EGESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSNEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. NCBI nr
Match: XP_023004277.1 (deoxyhypusine hydroxylase-B [Cucurbita maxima] >XP_023004278.1 deoxyhypusine hydroxylase-B [Cucurbita maxima] >XP_023004279.1 deoxyhypusine hydroxylase-B [Cucurbita maxima])

HSP 1 Score: 603.2 bits (1554), Expect = 1.3e-168
Identity = 316/320 (98.75%), Postives = 318/320 (99.38%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVN DAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT
Sbjct: 37  MAFVNGDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 96

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIP+L
Sbjct: 97  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPFL 156

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSS +GESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 157 EKSLALDPAQEVKETCELALKRIEQLKDSSNDGESSTVEKSPFLSVDPAAPASSDSSVCQ 216

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 217 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 276

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML
Sbjct: 277 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 336

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 337 EYERLGRSFEFLFMQSPQVQ 356

[Close]
BLAST of CmoCh08G002680 vs. NCBI nr
Match: XP_023550138.1 (deoxyhypusine hydroxylase-B [Cucurbita pepo subsp. pepo] >XP_023550139.1 deoxyhypusine hydroxylase-B [Cucurbita pepo subsp. pepo] >XP_023550140.1 deoxyhypusine hydroxylase-B [Cucurbita pepo subsp. pepo] >XP_023550141.1 deoxyhypusine hydroxylase-B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.4 bits (1544), Expect = 1.9e-167
Identity = 315/320 (98.44%), Postives = 316/320 (98.75%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MAFVN DAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPR+ALILAT
Sbjct: 1   MAFVNGDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPREALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNI YL
Sbjct: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNISYL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELALKRIEQLKDSS EGESSTVEKSPFLSVDPAAPASSDSSVCQ
Sbjct: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSNEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA
Sbjct: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALL EFAKDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLNEFAKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. NCBI nr
Match: XP_038897138.1 (deoxyhypusine hydroxylase [Benincasa hispida])

HSP 1 Score: 555.8 bits (1431), Expect = 2.4e-154
Identity = 292/320 (91.25%), Postives = 306/320 (95.62%), Query Frame = 0 

Query: 1   MAFVNDDAEITSFACSSEMEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILAT 60
           MA VN D EI+SF+CSS+MEKFLCDRLND S PIAERFRALFSLRNLKGP PR+ALILAT
Sbjct: 1   MASVNGDGEISSFSCSSDMEKFLCDRLNDVSLPIAERFRALFSLRNLKGPTPRNALILAT 60

Query: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYL 120
           RDPSNLLAHEAAFALGQMQDTDAIPALV VLNDLSLHPIVRHEAAEALGAIG ESNIP+L
Sbjct: 61  RDPSNLLAHEAAFALGQMQDTDAIPALVVVLNDLSLHPIVRHEAAEALGAIGLESNIPFL 120

Query: 121 EKSLALDPAQEVKETCELALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQ 180
           EKSLALDPAQEVKETCELAL+RIEQLKDS+ EGESSTVEKSPF+SVDPAAPASS++SV Q
Sbjct: 121 EKSLALDPAQEVKETCELALRRIEQLKDSANEGESSTVEKSPFMSVDPAAPASSNASVGQ 180

Query: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKA 240
           LREILLDDDKGMYERYAALFALRNNGNDEALTAII+SL S SALLKHEVAYVLGQLQ KA
Sbjct: 181 LREILLDDDKGMYERYAALFALRNNGNDEALTAIIDSLSSTSALLKHEVAYVLGQLQKKA 240

Query: 241 ASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSML 300
           ASDALS+ILEDV EHPMVRHEAAEALGSIADE+SIALLKEF+KDPEPIVSQSCEVALSML
Sbjct: 241 ASDALSDILEDVKEHPMVRHEAAEALGSIADEKSIALLKEFSKDPEPIVSQSCEVALSML 300

Query: 301 EYERLGRSFEFLFMQSPQVQ 321
           EYERLGRSFEFLFMQSPQVQ
Sbjct: 301 EYERLGRSFEFLFMQSPQVQ 320

[Close]
BLAST of CmoCh08G002680 vs. TAIR 10
Match: AT3G58180.1 (ARM repeat superfamily protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-123
Identity = 227/301 (75.42%), Postives = 263/301 (87.38%), Query Frame = 0 

Query: 19  MEKFLCDRLNDPSQPIAERFRALFSLRNLKGPAPRDALILATRDPSNLLAHEAAFALGQM 78
           +EKFLC+RL D SQPI+ERFRALFSLRNLKGP PR+ALILA+RD SNLLAHEAAFALGQM
Sbjct: 13  LEKFLCERLVDQSQPISERFRALFSLRNLKGPGPRNALILASRDSSNLLAHEAAFALGQM 72

Query: 79  QDTDAIPALVTVLNDLSLHPIVRHEAAEALGAIGSESNIPYLEKSLALDPAQEVKETCEL 138
           QD +AIPAL +VLND+SLHPIVRHEAAEALGAIG   N+  L+KSL+ DPAQEV+ETCEL
Sbjct: 73  QDAEAIPALESVLNDMSLHPIVRHEAAEALGAIGLAGNVNILKKSLSSDPAQEVRETCEL 132

Query: 139 ALKRIEQLKDSSKEGESSTVEKSPFLSVDPAAPASSDSSVCQLREILLDDDKGMYERYAA 198
           ALKRIE + +   E +SST EKSPF+SVDPA PA+S SSV QLR++LLD+ KGMYERYAA
Sbjct: 133 ALKRIEDMSNVDAENQSSTTEKSPFMSVDPAGPAASFSSVHQLRQVLLDETKGMYERYAA 192

Query: 199 LFALRNNGNDEALTAIINSLRSKSALLKHEVAYVLGQLQNKAASDALSNILEDVNEHPMV 258
           LFALRN+G +EA++AI++SL + SALL+HEVAYVLGQLQ+K A   LS +L DVNEHPMV
Sbjct: 193 LFALRNHGGEEAVSAIVDSLSASSALLRHEVAYVLGQLQSKTALATLSKVLRDVNEHPMV 252

Query: 259 RHEAAEALGSIADERSIALLKEFAKDPEPIVSQSCEVALSMLEYERLGRSFEFLFMQSPQ 318
           RHEAAEALGSIADE+SIALL+EF+KDPEPIV+QSCEVALSMLE+E  G+SFEF F Q P 
Sbjct: 253 RHEAAEALGSIADEQSIALLEEFSKDPEPIVAQSCEVALSMLEFENSGKSFEFFFTQDPL 312

Query: 319 V 320
           V
Sbjct: 313 V 313

[Close]
BLAST of CmoCh08G002680 vs. TAIR 10
Match: AT3G62530.1 (ARM repeat superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 8.7e-30
Identity = 81/199 (40.70%), Postives = 124/199 (62.31%), Query Frame = 0 

Query: 114 ESNIPYLEKSLALDPAQEVKETCELAL-KRIEQLKDSSKEGESSTVEKSPFLSVDPAAPA 173
           ES +P L  SL+    Q  K T       +I ++     E + +T   +   ++D +APA
Sbjct: 14  ESRLPLLRLSLSKCLDQSFKTTASSEYGSKINKVTKHVNEEDGATFYVTRRGAIDSSAPA 73

Query: 174 SSD--------SSVCQLREILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSAL 233
            S         SS+  +RE LL++ +  Y R  ALFALRN+G ++A+  II SL  +S++
Sbjct: 74  ESKAYPKAANISSIHSMRESLLEETEEDYYRRLALFALRNHGGEDAINVIIESLGVESSM 133

Query: 234 LKHEVAYVLGQLQNKAASDALSNILEDVNEHPMVRHEAAEALGSIADERSIALLKEFAKD 293
           ++ E A+VLGQL++K A  +LS IL DV EHPMVR EAA+ALG IADE+S  +L+E + D
Sbjct: 134 IRIEAAFVLGQLESKTAIASLSKILRDVKEHPMVRVEAAKALGFIADEKSREVLQELSGD 193

Query: 294 PEPIVSQSCEVALSMLEYE 304
            +PI+++ C+ +LS+LE++
Sbjct: 194 LDPIIAKGCDSSLSILEFK 212

[Close]
BLAST of CmoCh08G002680 vs. TAIR 10
Match: AT3G43260.1 (BEST Arabidopsis thaliana protein match is: ARM repeat superfamily protein (TAIR:AT3G62530.1); Has 64 Blast hits to 58 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 8; Fungi - 0; Plants - 53; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 42.7 bits (99), Expect = 6.3e-04
Identity = 20/51 (39.22%), Postives = 35/51 (68.63%), Query Frame = 0 

Query: 182 REILLDDDKGMYERYAALFALRNNGNDEALTAIINSLRSKSALLKHEVAYV 233
           RE LL++ + +Y+R  ALFAL + G ++A+  I+ SL  +S+++  E A+V
Sbjct: 208 RESLLEETEEIYQRCLALFALMHQGGEDAVNVIVESLGVESSMIHIEAAFV 258

The following BLAST results are available for this feature:
BLAST of CmoCh08G002680 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94JW01.9e-12275.42Deoxyhypusine hydroxylase OS=Arabidopsis thaliana OX=3702 GN=At3g58180 PE=1 SV=1[more]
Q2QXB33.4e-11974.12Deoxyhypusine hydroxylase-A OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g016... [more]
Q2QLW33.5e-11675.17Deoxyhypusine hydroxylase-B OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g062... [more]
Q5ZIP36.2e-6549.82Deoxyhypusine hydroxylase OS=Gallus gallus OX=9031 GN=DOHH PE=2 SV=1[more]
Q9BU894.0e-6451.23Deoxyhypusine hydroxylase OS=Homo sapiens OX=9606 GN=DOHH PE=1 SV=1[more]
BLAST of CmoCh08G002680 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H7863.9e-171100.00Deoxyhypusine hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111460813 PE=3 SV=... [more]
A0A6J1KPZ86.3e-16998.75Deoxyhypusine hydroxylase OS=Cucurbita maxima OX=3661 GN=LOC111497653 PE=3 SV=1[more]
A0A1S3BGF37.7e-15189.06Deoxyhypusine hydroxylase OS=Cucumis melo OX=3656 GN=LOC103489734 PE=3 SV=1[more]
A0A0A0K8K51.7e-15089.06Deoxyhypusine hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_7G372960 PE=3 SV=1[more]
A0A6J1DQT95.0e-15092.81Deoxyhypusine hydroxylase OS=Momordica charantia OX=3673 GN=LOC111023477 PE=3 SV... [more]
BLAST of CmoCh08G002680 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022959892.18.1e-171100.00deoxyhypusine hydroxylase [Cucurbita moschata] >XP_022959893.1 deoxyhypusine hyd... [more]
KAG6593054.17.6e-16999.06Deoxyhypusine hydroxylase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_023004277.11.3e-16898.75deoxyhypusine hydroxylase-B [Cucurbita maxima] >XP_023004278.1 deoxyhypusine hyd... [more]
XP_023550138.11.9e-16798.44deoxyhypusine hydroxylase-B [Cucurbita pepo subsp. pepo] >XP_023550139.1 deoxyhy... [more]
XP_038897138.12.4e-15491.25deoxyhypusine hydroxylase [Benincasa hispida][more]
BLAST of CmoCh08G002680 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G58180.11.4e-12375.42ARM repeat superfamily protein [more]
AT3G62530.18.7e-3040.70ARM repeat superfamily protein [more]
AT3G43260.16.3e-0439.22BEST Arabidopsis thaliana protein match is: ARM repeat superfamily protein (TAIR... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..149
NoneNo IPR availablePFAMPF13646HEAT_2coord: 55..141
e-value: 3.3E-15
score: 56.2
coord: 226..297
e-value: 3.6E-10
score: 40.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..174
NoneNo IPR availablePANTHERPTHR12697:SF5DEOXYHYPUSINE HYDROXYLASEcoord: 15..317
NoneNo IPR availablePANTHERPTHR12697PBS LYASE HEAT-LIKE PROTEINcoord: 15..317
IPR004155PBS lyase HEAT-like repeatSMARTSM00567EZ_HEATcoord: 192..221
e-value: 0.0097
score: 25.1
coord: 65..94
e-value: 2.0E-5
score: 34.0
coord: 256..285
e-value: 1.7E-8
score: 44.2
coord: 223..252
e-value: 0.0022
score: 27.2
coord: 34..63
e-value: 0.77
score: 18.8
coord: 98..127
e-value: 0.0036
score: 26.5
IPR004155PBS lyase HEAT-like repeatPFAMPF03130HEAT_PBScoord: 195..218
e-value: 0.051
score: 14.3
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 8..161
e-value: 3.7E-22
score: 80.8
coord: 167..311
e-value: 1.6E-21
score: 78.7
IPR027517Deoxyhypusine hydroxylaseHAMAPMF_03101Deoxyhypusine_hydroxylasecoord: 16..310
score: 33.004333
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 97..299
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 17..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G002680.1CmoCh08G002680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008612 peptidyl-lysine modification to peptidyl-hypusine
molecular_function GO:0019135 deoxyhypusine monooxygenase activity
molecular_function GO:0046872 metal ion binding